YRC Public Data Repository - Protein Structure Prediction

Protein:	THII_ECOBW
Organism:	Escherichia coli BW2952
Length:	482 amino acids
Reference:	Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for THII_ECOBW.

Description	E-value	Query Range	Subject Range
gi\|213427860 - gi\|213427860\|ref\|ZP_03360610.1\| thiamine biosynthesis protein ThiI [Salmonella enterica subsp. enter... THII_SALTI - tRNA sulfurtransferase OS=Salmonella typhi GN=thiI PE=3 SV=1 gi\|213052897 - gi\|213052897\|ref\|ZP_03345775.1\| thiamine biosynthesis protein ThiI [Salmonella enterica subsp. enter... THII_SALTI - Thiamine biosynthesis protein thiI OS=Salmonella typhi GN=thiI PE=3 SV=1 gi\|214000382 - gi\|214000382\|ref\|ZP_03411117.1\| thiamine biosynthesis protein ThiI [Salmonella enterica subsp. enter... gi\|213855373 - gi\|213855373\|ref\|ZP_03383613.1\| thiamine biosynthesis protein ThiI [Salmonella enterica subsp. enter... gi\|29142826, gi\|... - gi\|29142826\|ref\|NP_806168.1\| thiamine biosynthesis protein ThiI [Salmonella enterica subsp. enterica... gi\|213647261 - gi\|213647261\|ref\|ZP_03377314.1\| thiamine biosynthesis protein ThiI [Salmonella enterica subsp. enter... gi\|16759402 - gi\|16759402\|ref\|NP_455019.1\| thiamine biosynthesis protein ThiI [Salmonella enterica subsp. enterica... gi\|213585097 - gi\|213585097\|ref\|ZP_03366923.1\| thiamine biosynthesis protein ThiI [Salmonella enterica subsp. enter...	382.0	[0..1]	[482..1]
gi\|204926685, gi... - gi\|204926685\|ref\|ZP_03217887.1\| thiamine biosynthesis/tRNA modification protein ThiI [Salmonella ent... THII_SALPK - tRNA sulfurtransferase OS=Salmonella paratyphi A (strain AKU_12601) GN=thiI PE=3 SV=1 gi\|197266005, gi... - gi\|197266005\|ref\|ZP_03166079.1\| thiamine biosynthesis/tRNA modification protein ThiI [Salmonella ent... THII_SALPA - tRNA sulfurtransferase OS=Salmonella paratyphi A GN=thiI PE=3 SV=1 THII_SALA4 - tRNA sulfurtransferase OS=Salmonella agona (strain SL483) GN=thiI PE=3 SV=1 THII_SALSV - tRNA sulfurtransferase OS=Salmonella schwarzengrund (strain CVM19633) GN=thiI PE=3 SV=1 gi\|168237538, gi... - gi\|197289454\|gb\|EDY28817.1\| thiamine biosynthesis/tRNA modification protein ThiI [Salmonella enteric... gi\|238911442 - gi\|238911442\|ref\|ZP_04655279.1\| thiamine biosynthesis protein ThiI [Salmonella enterica subsp. enter... THII_SALPA - tRNA sulfurtransferase OS=Salmonella paratyphi A (strain ATCC 9150 / SARB42) GN=thiI PE=3 SV=1	379.0	[0..1]	[482..1]
THII_SALCH - tRNA sulfurtransferase OS=Salmonella choleraesuis (strain SC-B67) GN=thiI PE=3 SV=1 THII_SALCH - Thiamine biosynthesis protein thiI OS=Salmonella choleraesuis GN=thiI PE=3 SV=1 THII_SALCH - tRNA sulfurtransferase OS=Salmonella choleraesuis GN=thiI PE=3 SV=1	379.0	[0..1]	[482..1]
lcl\|NC_016856.1_... - [gene=thiI] [protein=thiamine biosynthesis protein ThiI] [protein_id=YP_005236118.1] [location=47856... THII_SALTY - tRNA sulfurtransferase OS=Salmonella typhimurium GN=thiI PE=3 SV=2 THII_SALTY - Thiamine biosynthesis protein thiI OS=Salmonella typhimurium GN=thiI PE=3 SV=2 THII_SALTY - tRNA sulfurtransferase OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) GN=thiI PE=3 ... gi\|205329560, gi... - gi\|205329560\|gb\|EDZ16324.1\| thiamine biosynthesis/tRNA modification protein ThiI [Salmonella enteric...	378.0	[0..1]	[482..1]
gi\|209743998, gi... - pir\|\|A85539 probable oxidoreductase yajK [imported] - Escherichia coli (strain O157:H7, substrain E... gi\|254591007, gi... - gi\|254791607\|ref\|YP_003076444.1\| sulfur transfer protein [Escherichia coli O157:H7 str. TW14359], gi... gi\|15800153, gi\|... - gi\|15800153\|ref\|NP_286165.1\| thiamine biosynthesis protein ThiI [Escherichia coli O157:H7 EDL933], g... gi\|208808957, gi... - gi\|208808957\|ref\|ZP_03251294.1\| thiamine biosynthesis/tRNA modification protein ThiI [Escherichia co... gi\|195938510 - gi\|195938510\|ref\|ZP_03083892.1\| thiamine biosynthesis protein ThiI [Escherichia coli O157:H7 str. EC... THII_ECO57 - tRNA sulfurtransferase OS=Escherichia coli O157:H7 GN=thiI PE=1 SV=1 gi\|168800306, gi... - gi\|189377419\|gb\|EDU95835.1\| thiamine biosynthesis/tRNA modification protein ThiI [Escherichia coli O... gi\|189373595, gi... - gi\|189373595\|gb\|EDU92011.1\| thiamine biosynthesis/tRNA modification protein ThiI [Escherichia coli O... gi\|217325977, gi... - gi\|217325977\|ref\|ZP_03442061.1\| thiamine biosynthesis/tRNA modification protein ThiI [Escherichia co... THII_ECO5E - tRNA sulfurtransferase OS=Escherichia coli O157:H7 (strain EC4115 / EHEC) GN=thiI PE=3 SV=1 gi\|208822980, gi... - gi\|208822980\|ref\|ZP_03263298.1\| thiamine biosynthesis/tRNA modification protein ThiI [Escherichia co... gi\|208731895, gi... - gi\|208815247\|ref\|ZP_03256426.1\| thiamine biosynthesis/tRNA modification protein ThiI [Escherichia co... gi\|189356012, gi... - gi\|189356012\|gb\|EDU74431.1\| thiamine biosynthesis/tRNA modification protein ThiI [Escherichia coli O... gi\|168749885, gi... - gi\|188015859\|gb\|EDU53981.1\| thiamine biosynthesis/tRNA modification protein ThiI [Escherichia coli O... gi\|188999332, gi... - gi\|188999332\|gb\|EDU68318.1\| thiamine biosynthesis/tRNA modification protein ThiI [Escherichia coli O... gi\|187768273, gi... - gi\|187768273\|gb\|EDU32117.1\| thiamine biosynthesis/tRNA modification protein ThiI [Escherichia coli O... gi\|13359934, gi\|... - gi\|15829731\|ref\|NP_308504.1\| thiamine biosynthesis protein ThiI [Escherichia coli O157:H7 str. Sakai... gi\|189362457, gi... - gi\|189362457\|gb\|EDU80876.1\| thiamine biosynthesis/tRNA modification protein ThiI [Escherichia coli O... gi\|189368047, gi... - gi\|189368047\|gb\|EDU86463.1\| thiamine biosynthesis/tRNA modification protein ThiI [Escherichia coli O...	376.0	[0..1]	[482..1]
THII_SHIF8 - tRNA sulfurtransferase OS=Shigella flexneri serotype 5b (strain 8401) GN=thiI PE=3 SV=1 THII_SHIFL - tRNA sulfurtransferase OS=Shigella flexneri GN=thiI PE=3 SV=2 gi\|30061917, gi\|... - gi\|30061917\|ref\|NP_836088.1\| thiamine biosynthesis protein ThiI [Shigella flexneri 2a str. 2457T], g...	376.0	[0..1]	[482..1]
THII_ERWCT - tRNA sulfurtransferase OS=Erwinia carotovora subsp. atroseptica GN=thiI PE=3 SV=1 THII_PECAS - tRNA sulfurtransferase OS=Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) GN=thiI PE=3...	376.0	[0..1]	[482..1]
THII_SHISS - tRNA sulfurtransferase OS=Shigella sonnei (strain Ss046) GN=thiI PE=3 SV=1	376.0	[0..1]	[482..1]
gi\|194432608, gi... - gi\|83570309\|ref\|ZP_00921754.1\| COG0301: Thiamine biosynthesis ATP pyrophosphatase [Shigella dysenter...	376.0	[0..1]	[482..1]
gi\|254287293, gi... - gi\|254287293\|ref\|YP_003053041.1\| thiamine biosynthesis protein ThiI [Escherichia coli BL21(DE3)], gi... gi\|242376201, gi... - gi\|251783928\|ref\|YP_002998232.1\| hypothetical protein B21_00375 [Escherichia coli BL21], gi\|24237620... gi\|254160489, gi... - gi\|254160489\|ref\|YP_003043597.1\| thiamine biosynthesis protein ThiI [Escherichia coli B str. REL606]... gi\|75237195, gi\|... - gi\|75237195\|ref\|ZP_00721242.1\| COG0301: Thiamine biosynthesis ATP pyrophosphatase [Escherichia coli ... THII_ECOHS - tRNA sulfurtransferase OS=Escherichia coli O9:H4 (strain HS) GN=thiI PE=3 SV=1 gi\|194438886, gi... - gi\|83585658\|ref\|ZP_00924300.1\| COG0301: Thiamine biosynthesis ATP pyrophosphatase [Escherichia coli ...	375.0	[0..1]	[482..1]

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Predicted Domain #1

Region A:
Residues: [1-355]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MKFIIKLFPE ITIKSQSVRL RFIKILTGNI RNVLKHYDET LAVVRHWDNI EVRAKDENQR  60
   61 LAIRDALTRI PGIHHILEVE DVPFTDMHDI FEKALVQYRD QLEGKTFCVR VKRRGKHDFS 120
  121 SIDVERYVGG GLNQHIESAR VKLTNPDVTV HLEVEDDRLL LIKGRYEGIG GFPIGTQEDV 180
  181 LSLISGGFDS GVSSYMLMRR GCRVHYCFFN LGGAAHEIGV RQVAHYLWNR FGSSHRVRFV 240
  241 AINFEPVVGE ILEKIDDGQM GVILKRMMVR AASKVAERYG VQALVTGEAL GQVSSQTLTN 300
  301 LRLIDNVSDT LILRPLISYD KEHIINLARQ IGTEDFARTM PEYCGVISKS PTVKA

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	71.0
Match:	2c5sA
Description:	Crystal structure of Bacillus anthracis ThiI, a tRNA-modifying enzyme containing the predicted RNA-binding THUMP domain
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
catalytic activity	0.735352182450617	bayes_pls_golite062009
ligase activity	0.249728764626579	bayes_pls_golite062009
ligase activity, forming carbon-nitrogen bonds	0.196167461233561	bayes_pls_golite062009
NAD+ synthase (glutamine-hydrolyzing) activity	0.1036205758202	bayes_pls_golite062009

Predicted Domain #2

Region A:
Residues: [356-482]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 VKSKIEAEEE KFDFSILDKV VEEANNVDIR EIAQQTEQEV VEVETVNGFG PNDVILDIRS  60
   61 IDEQEDKPLK VEGIDVVSLP FYKLSTKFGD LDQNKTWLLW CERGVMSRLQ ALYLREQGFN 120
  121 NVKVYRP

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	40.522879
Match:	2hmaA
Description:	No description for 2hmaA was found.

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: