Bait Protein: | ASF1 |
Organism: | Saccharomyces cerevisiae |
Publication: | Green EM, Antczak AJ, Bailey AO, Franco AA, Wu KJ, Yates JR 3rd, Kaufman PD. Replication-independent histone deposition by the HIR complex and Asf1. Curr Biol. 2005 Nov 22;15(22):2044-9. |
Comments: | sample 3928 - Asf1-S-TEV-ZZ (in hir3delta mutant) |
Raw Data: |
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FILTERED Run Results:
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Hit Protein | Protein Desc | Sequence Count | Spectrum Count | Sequence Coverage | NSAF | Mol. Wt. | |
[ |
ASF1 | Nucleosome assembly factor, involved in chromatin assembly and disassembly, anti-silencing protein t... | 38 | 118 | 56.6% | 0.0649 | 31603 |
[ |
EFB1 | Translation elongation factor 1 beta; stimulates nucleotide exchange to regenerate EF-1 alpha-GTP fo... | 9 | 11 | 39.3% | 0.00819 | 22627 |
[ |
TEF1, TEF2 | Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction ... | 20 | 47 | 37.3% | 0.0157 | 50033 |
[ |
RAD53 | Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autopho... | 26 | 31 | 36.9% | 0.00579 | 91962 |
[ |
RPP1A | Ribosomal stalk protein P1 alpha, involved in the interaction between translational elongation facto... | 2 | 5 | 32.1% | 0.00724 | 10908 |
[ |
CDC33 | Cytoplasmic mRNA cap binding protein and translation initiation factor eIF4E; the eIF4E-cap complex ... | 5 | 5 | 28.6% | 0.0036 | 24254 |
[ |
MLC1 | Essential light chain for Myo1p, light chain for Myo2p; stabilizes Myo2p by binding to the neck regi... | 2 | 2 | 27.5% | 0.00206 | 16444 |
[ |
STM1 | Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with r... | 6 | 7 | 22.0% | 0.00393 | 29995 |
[ |
DCD1 | Deoxycytidine monophosphate (dCMP) deaminase required for dCTP and dTTP synthesis; expression is NOT... | 2 | 2 | 20.5% | 9.84E-4 | 35646 |
[ |
IBI2 | Protein of unknown function that associates with ribosomes; green fluorescent protein (GFP)-fusion p... | 2 | 2 | 17.8% | 0.00156 | 22179 |
[ |
SRO9 | Cytoplasmic RNA-binding protein that associates with translating ribosomes; involved in heme regulat... | 4 | 4 | 17.1% | 0.00141 | 48060 |
[ |
VMA2 | Subunit B of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), a... | 5 | 5 | 16.4% | 0.00148 | 57749 |
[ |
YDJ1 | Protein chaperone involved in regulation of the HSP90 and HSP70 functions; involved in protein trans... | 5 | 5 | 13.2% | 0.00188 | 44671 |
[ |
ARC1 | Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetases (Mes1p and Gus1p), delivering t... | 3 | 3 | 12.8% | 0.00122 | 42084 |
[ |
PMA1 | Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plas... | 5 | 6 | 12.1% | 0.001 | 99619 |
[ |
YHR020W | Protein of unknown function that may interact with ribosomes, based on co-purification experiments; ... | 5 | 5 | 11.9% | 0.00112 | 77386 |
[ |
DED1 | ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all ye... | 3 | 4 | 10.1% | 0.00102 | 65553 |
[ |
NPL3 | RNA-binding protein that promotes elongation, regulates termination, and carries poly(A) mRNA from n... | 2 | 3 | 9.4% | 0.00111 | 45407 |
[ |
SSZ1 | Hsp70 protein that interacts with Zuo1p (a DnaJ homolog) to form a ribosome-associated complex that ... | 3 | 3 | 8.7% | 8.56E-4 | 58238 |
[ |
AZR1 | Plasma membrane transporter of the major facilitator superfamily, involved in resistance to azole dr... | 2 | 2 | 8.6% | 5.01E-4 | 67169 |
[ |
NSR1 | Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ri... | 3 | 3 | 8.2% | 0.00111 | 44535 |
[ |
PMA2 | Plasma membrane H+-ATPase, isoform of Pma1p, involved in pumping protons out of the cell; regulator ... | 3 | 4 | 7.5% | 6.48E-4 | 102172 |
[ |
SLX8 | Subunit of the Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex; stimulated by prior attachm... | 2 | 3 | 7.3% | 0.00168 | 30764 |
[ |
BFR2 | Essential protein that is a component of 90S preribosomes; may be involved in rRNA processing; multi... | 2 | 2 | 7.1% | 5.75E-4 | 61203 |
[ |
AVT4 | Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seve... | 2 | 2 | 6.6% | 4.3E-4 | 80026 |
[ |
GFA1 | Glutamine-fructose-6-phosphate amidotransferase, catalyzes the formation of glucosamine-6-P and glut... | 2 | 2 | 6.4% | 4.28E-4 | 80047 |
[ |
HIR2 | Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene tra... | 3 | 3 | 6.3% | 5.26E-4 | 98445 |
[ |
DBP2 | Essential ATP-dependent RNA helicase of the DEAD-box protein family, involved in nonsense-mediated m... | 2 | 2 | 5.5% | 5.62E-4 | 60999 |
[ |
YEF3 | Translational elongation factor 3, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes b... | 2 | 2 | 3.0% | 2.94E-4 | 115945 |
[ |
YLR001C | Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purifi... | 2 | 2 | 1.3% | 3.56E-4 | 97618 |