Bait Protein: | DAD1 |
Organism: | Saccharomyces cerevisiae |
Publication: | Shimogawa M. M. et al. (2006) Mps1 phosphorylation of Dam1 couples kinetochores to microtubule plus ends at metaphase. Curr Biol. 2006 Aug 8;16(15):1489-501. |
Comments: | Dam1 complex purified from yeast with Dad1-TAP |
Raw Data: |
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FILTERED Run Results:
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Hit Protein | Protein Desc | Sequence Count | Spectrum Count | Sequence Coverage | NSAF | Mol. Wt. | |
[ |
HSK3 | Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced... | 3 | 4 | 30.4% | 0.0177 | 8088 |
[ |
DAD1 | Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced... | 7 | 17 | 62.8% | 0.0552 | 10516 |
[ |
DAD3 | Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced... | 7 | 13 | 59.6% | 0.0422 | 10848 |
[ |
SPC19 | Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced... | 10 | 96 | 67.3% | 0.178 | 18910 |
[ |
VMA4 | Subunit E of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), a... | 5 | 5 | 23.2% | 0.00655 | 26471 |
[ |
DUO1 | Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced... | 13 | 25 | 58.7% | 0.0309 | 27473 |
[ |
MRPS17 | Mitochondrial ribosomal protein of the small subunit | 2 | 2 | 10.5% | 0.00258 | 27635 |
[ |
CIR1 | Protein that interacts with frataxin (Yfh1p); putative homolog of mammalian electron transfer flavop... | 2 | 2 | 14.6% | 0.00234 | 28759 |
[ |
MRPL13 | Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation | 2 | 2 | 11.4% | 0.00231 | 30271 |
[ |
ASK1 | Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced... | 19 | 42 | 63.0% | 0.0439 | 32056 |
[ |
SPC34 | Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced... | 16 | 41 | 51.2% | 0.0425 | 34078 |
[ |
ATP3 | Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily ... | 5 | 6 | 19.9% | 0.00589 | 34351 |
[ |
PET9 | Major ADP/ATP carrier of the mitochondrial inner membrane, exchanges cytosolic ADP for mitochondrial... | 2 | 2 | 9.7% | 0.00192 | 34426 |
[ |
ADH1 | Alcohol dehydrogenase, fermentative isozyme active as homo- or heterotetramers; required for the red... | 4 | 4 | 17.2% | 0.00351 | 36849 |
[ |
RPC40 | RNA polymerase subunit AC40, common to RNA polymerase I and III | 2 | 3 | 9.6% | 0.00274 | 37687 |
[ |
DAM1 | Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced... | 19 | 36 | 44.6% | 0.0321 | 38422 |
[ |
PSA1 | GDP-mannose pyrophosphorylase (mannose-1-phosphate guanyltransferase), synthesizes GDP-mannose from ... | 6 | 7 | 24.1% | 0.00592 | 39566 |
[ |
ACT1 | Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functio... | 2 | 2 | 7.7% | 0.00163 | 41690 |
[ |
SPC42 | Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate att... | 3 | 33 | 9.9% | 0.0278 | 42271 |
[ |
ENO2 | Enolase II, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosp... | 3 | 3 | 11.9% | 0.0021 | 46914 |
[ |
TUB3 | Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form... | 2 | 2 | 8.5% | 0.00137 | 49694 |
[ |
TUB1 | Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form... | 2 | 2 | 8.5% | 0.00137 | 49800 |
[ |
TEF1, TEF2 | Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction ... | 3 | 5 | 14.8% | 0.00333 | 50033 |
[ |
LAT1 | Dihydrolipoamide acetyltransferase component (E2) of pyruvate dehydrogenase complex, which catalyzes... | 2 | 2 | 6.6% | 0.00127 | 51818 |
[ |
CDC19 | Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvat... | 14 | 16 | 28.6% | 0.00977 | 54545 |
[ |
CYS4 | Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the ... | 7 | 7 | 24.3% | 0.00422 | 56022 |
[ |
VMA2 | Subunit B of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), a... | 4 | 4 | 12.8% | 0.00236 | 57749 |
[ |
PGM1 | Phosphoglucomutase, minor isoform; catalyzes the conversion from glucose-1-phosphate to glucose-6-ph... | 2 | 2 | 4.0% | 0.00107 | 63112 |
[ |
ILV2 | Acetolactate synthase, catalyses the first common step in isoleucine and valine biosynthesis and is ... | 4 | 4 | 7.9% | 0.00178 | 74937 |
[ |
GFA1 | Glutamine-fructose-6-phosphate amidotransferase, catalyzes the formation of glucosamine-6-P and glut... | 2 | 2 | 6.0% | 8.52E-4 | 80047 |
[ |
EFT2, EFT1 | Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein s... | 6 | 8 | 11.4% | 0.0029 | 93289 |
[ |
PMA1 | Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plas... | 8 | 10 | 15.8% | 0.00333 | 99619 |