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Bait Protein: DAD1
Organism: Saccharomyces cerevisiae
Publication: Shimogawa M. M. et al. (2006) Mps1 phosphorylation of Dam1 couples kinetochores to microtubule plus ends at metaphase. Curr Biol. 2006 Aug 8;16(15):1489-501.
Comments: Dam1 complex purified from yeast with Dad1-TAP

Raw Data:

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FILTERED Run Results: [unfilter]

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  Hit Protein Protein Desc Sequence Count Spectrum Count Sequence Coverage NSAF Mol. Wt.
[View Peptides]   SPC19 Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced... 10 96 67.3% 0.178 18910
[View Peptides]   ASK1 Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced... 19 42 63.0% 0.0439 32056
[View Peptides]   DAD1 Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced... 7 17 62.8% 0.0552 10516
[View Peptides]   DAD3 Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced... 7 13 59.6% 0.0422 10848
[View Peptides]   DUO1 Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced... 13 25 58.7% 0.0309 27473
[View Peptides]   SPC34 Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced... 16 41 51.2% 0.0425 34078
[View Peptides]   DAM1 Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced... 19 36 44.6% 0.0321 38422
[View Peptides]   HSK3 Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced... 3 4 30.4% 0.0177 8088
[View Peptides]   CDC19 Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvat... 14 16 28.6% 0.00977 54545
[View Peptides]   CYS4 Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the ... 7 7 24.3% 0.00422 56022
[View Peptides]   PSA1 GDP-mannose pyrophosphorylase (mannose-1-phosphate guanyltransferase), synthesizes GDP-mannose from ... 6 7 24.1% 0.00592 39566
[View Peptides]   VMA4 Subunit E of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), a... 5 5 23.2% 0.00655 26471
[View Peptides]   ATP3 Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily ... 5 6 19.9% 0.00589 34351
[View Peptides]   ADH1 Alcohol dehydrogenase, fermentative isozyme active as homo- or heterotetramers; required for the red... 4 4 17.2% 0.00351 36849
[View Peptides]   PMA1 Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plas... 8 10 15.8% 0.00333 99619
[View Peptides]   TEF1, TEF2 Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction ... 3 5 14.8% 0.00333 50033
[View Peptides]   CIR1 Protein that interacts with frataxin (Yfh1p); putative homolog of mammalian electron transfer flavop... 2 2 14.6% 0.00234 28759
[View Peptides]   VMA2 Subunit B of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), a... 4 4 12.8% 0.00236 57749
[View Peptides]   ENO2 Enolase II, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosp... 3 3 11.9% 0.0021 46914
[View Peptides]   EFT2, EFT1 Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein s... 6 8 11.4% 0.0029 93289
[View Peptides]   MRPL13 Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation 2 2 11.4% 0.00231 30271
[View Peptides]   MRPS17 Mitochondrial ribosomal protein of the small subunit 2 2 10.5% 0.00258 27635
[View Peptides]   SPC42 Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate att... 3 33 9.9% 0.0278 42271
[View Peptides]   PET9 Major ADP/ATP carrier of the mitochondrial inner membrane, exchanges cytosolic ADP for mitochondrial... 2 2 9.7% 0.00192 34426
[View Peptides]   RPC40 RNA polymerase subunit AC40, common to RNA polymerase I and III 2 3 9.6% 0.00274 37687
[View Peptides]   TUB1 Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form... 2 2 8.5% 0.00137 49800
[View Peptides]   TUB3 Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form... 2 2 8.5% 0.00137 49694
[View Peptides]   ILV2 Acetolactate synthase, catalyses the first common step in isoleucine and valine biosynthesis and is ... 4 4 7.9% 0.00178 74937
[View Peptides]   ACT1 Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functio... 2 2 7.7% 0.00163 41690
[View Peptides]   LAT1 Dihydrolipoamide acetyltransferase component (E2) of pyruvate dehydrogenase complex, which catalyzes... 2 2 6.6% 0.00127 51818
[View Peptides]   GFA1 Glutamine-fructose-6-phosphate amidotransferase, catalyzes the formation of glucosamine-6-P and glut... 2 2 6.0% 8.52E-4 80047
[View Peptides]   PGM1 Phosphoglucomutase, minor isoform; catalyzes the conversion from glucose-1-phosphate to glucose-6-ph... 2 2 4.0% 0.00107 63112

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