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Run Information:

Bait Protein: VAM3
Organism: Saccharomyces cerevisiae
Publication: Hao Xu , Youngsoo Jun, James Thompson, John Yates and William Wickner (2010) HOPS Prevents the Disassembly of Trans-SNARE Complexes by Sec17p/Sec18p during Membrane Fusion [In Press]
Comments: hx21: both fusion and trans-SNARE complex formation take place. Investigating Vam3p, Nyv1p and their partners in trans-SNARE complex

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  Hit Protein Protein Desc Sequence Count Spectrum Count Sequence Coverage NSAF Mol. Wt.
[View Peptides]   VAM3 Syntaxin-related protein required for vacuolar assembly; functions with Vam7p in vacuolar protein tr... 12 33 56.9% 0.0246 32498
[View Peptides]   NYV1 v-SNARE component of the vacuolar SNARE complex involved in vesicle fusion; inhibits ATP-dependent C... 8 32 56.5% 0.0267 28964
[View Peptides]   VAM7 Component of the vacuole SNARE complex involved in vacuolar morphogenesis; SNAP-25 homolog; function... 12 49 42.4% 0.0327 36711
[View Peptides]   PHO8 Repressible alkaline phosphatase, a glycoprotein localized to the vacuole; regulated by levels of in... 16 43 37.6% 0.016 63004
[View Peptides]   VTI1 Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and ... 6 16 35.5% 0.0156 24668
[View Peptides]   YPF1 Putative protein of unknown function with similarity to a human minor histocompatibility antigen and... 6 9 15.2% 0.00324 67525
[View Peptides]   FRA1 Protein involved in negative regulation of transcription of iron regulon; forms an iron independent ... 7 7 13.5% 0.00197 84924
[View Peptides]   MAM3 Protein required for normal mitochondrial morphology, has similarity to hemolysins 3 7 11.3% 0.00209 77712
[View Peptides]   YPK9 Vacuolar protein with a possible role in sequestering heavy metals; has similarity to the type V P-t... 11 14 10.7% 0.00201 166749
[View Peptides]   MNR2 Vacuolar membrane protein required for magnesium homeostasis; putative magnesium transporter; has si... 6 8 10.2% 0.00174 109736
[View Peptides]   ZRC1 Vacuolar membrane zinc transporter, transports zinc from the cytosol into the vacuole for storage; a... 3 5 9.7% 0.00239 48345
[View Peptides]   YNL217W Hypothetical protein 2 3 8.6% 0.00194 37151
[View Peptides]   VNX1 Low affinity vacuolar membrane localized monovalent cation/H+ antiporter; member of the calcium exch... 4 5 7.4% 0.00116 102499
[View Peptides]   UGA1 Gamma-aminobutyrate (GABA) transaminase (4-aminobutyrate aminotransferase) involved in the 4-aminobu... 2 3 7.4% 0.00134 52946
[View Peptides]   FPS1 Plasma membrane channel, member of major intrinsic protein (MIP) family; involved in efflux of glyce... 4 4 7.2% 0.00126 73877
[View Peptides]   AVT3 Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seve... 2 3 6.8% 9.15E-4 75459
[View Peptides]   ZRT3 Vacuolar membrane zinc transporter, transports zinc from storage in the vacuole to the cytoplasm whe... 2 4 5.6% 0.00168 55403
[View Peptides]   CPS1 Vacuolar carboxypeptidase yscS; expression is induced under low-nitrogen conditions 2 3 4.5% 0.0011 64597
[View Peptides]   CDC19 Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvat... 2 2 4.4% 8.45E-4 54545
[View Peptides]   YCK3 Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fus... 2 2 4.2% 8.06E-4 60261
[View Peptides]   PHO91 Low-affinity phosphate transporter of the vacuolar membrane; deletion of pho84, pho87, pho89, pho90,... 3 4 3.8% 9.45E-4 99490
[View Peptides]   PHO84 High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated... 2 6 3.4% 0.00216 64382
[View Peptides]   YDR089W Protein of unknown function; deletion confers resistance to Nickel 2 2 3.1% 4.86E-4 98712
[View Peptides]   YCF1 Vacuolar glutathione S-conjugate transporter of the ATP-binding cassette family, has a role in detox... 3 3 2.5% 4.18E-4 171120

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