Bait Protein: | MLP2 |
Organism: | Saccharomyces cerevisiae |
Publication: | Keck JM, Jones MH, Wong CC, Binkley J, Chen D, Jaspersen SL, Holinger EP, Xu T, Niepel M, Rout MP, Vogel J, Sidow A, Yates JR, Winey M., A Cell Cycle Phosphoproteome of the Yeast Centrosome, Science 24 June 2011: 1557-1561. |
Comments: | #07 Alpha Factor Prep3-TiO2 Flowthrough |
Raw Data: |
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FILTERED Run Results:
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Hit Protein | Protein Desc | Sequence Count | Spectrum Count | Sequence Coverage | NSAF | Mol. Wt. | |
[ |
MLP2 | Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... | 184 | 220 | 69.6% | 0.127 | 195140 |
[ |
YIL002W-A | Putative protein of unknown function; identified by expression profiling and mass spectrometry | 8 | 11 | 65.2% | 0.155 | 7729 |
[ |
SPC42 | Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate att... | 11 | 11 | 42.4% | 0.0295 | 42271 |
[ |
PDC1 | Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates... | 16 | 19 | 36.9% | 0.0328 | 61495 |
[ |
RPP2A | Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction... | 2 | 2 | 29.2% | 0.0183 | 10746 |
[ |
ENO2 | Enolase II, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosp... | 9 | 9 | 28.8% | 0.02 | 46914 |
[ |
SPC29 | Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inn... | 6 | 6 | 28.5% | 0.0231 | 29280 |
[ |
CDC31 | Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication i... | 3 | 3 | 26.1% | 0.0181 | 18751 |
[ |
CMD1 | Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, act... | 3 | 3 | 24.5% | 0.0198 | 16135 |
[ |
EFT2, EFT1 | Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein s... | 18 | 18 | 23.4% | 0.0208 | 93289 |
[ |
SPC110 | Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nu... | 17 | 17 | 20.0% | 0.0175 | 111782 |
[ |
PMA1 | Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plas... | 12 | 13 | 19.5% | 0.0138 | 99619 |
[ |
STM1 | Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with r... | 4 | 4 | 19.4% | 0.0142 | 29995 |
[ |
ENO1 | Enolase I, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosph... | 6 | 6 | 18.8% | 0.0133 | 46816 |
[ |
CNM67 | Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclea... | 5 | 5 | 11.7% | 0.00837 | 67400 |
[ |
KAR2 | ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding i... | 4 | 4 | 10.6% | 0.0057 | 74468 |
[ |
NBP1 | Spindle pole body (SPB) component, required for the insertion of the duplication plaque into the nuc... | 4 | 4 | 9.4% | 0.0122 | 37354 |
[ |
MPS2 | Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required f... | 3 | 3 | 9.0% | 0.00754 | 44585 |
[ |
BBP1 | Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periph... | 2 | 2 | 8.1% | 0.00505 | 45384 |
[ |
BFA1 | Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pa... | 6 | 6 | 8.0% | 0.0102 | 66087 |
[ |
HSC82 | Cytoplasmic chaperone of the Hsp90 family, redundant in function and nearly identical with Hsp82p, a... | 4 | 4 | 7.2% | 0.00552 | 80900 |
[ |
MPP10 | Component of the SSU processome and 90S preribosome, required for pre-18S rRNA processing, interacts... | 3 | 3 | 6.2% | 0.00492 | 66953 |
[ |
NUD1 | Component of the spindle pole body outer plaque, required for exit from mitosis | 4 | 4 | 6.2% | 0.00457 | 94104 |
[ |
SPC97 | Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the... | 3 | 3 | 5.2% | 0.00354 | 96825 |
[ |
TCP1 | Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; inv... | 2 | 2 | 5.0% | 0.00348 | 60481 |
[ |
HEF3 | Translational elongation factor EF-3; paralog of YEF3 and member of the ABC superfamily; stimulates ... | 4 | 4 | 4.9% | 0.00373 | 115869 |
[ |
MLP1 | Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... | 6 | 6 | 4.3% | 0.00311 | 218454 |
[ |
SUP35 | Translation termination factor eRF3; altered protein conformation creates the [PSI(+)] prion, a domi... | 2 | 2 | 3.5% | 0.00284 | 76551 |
[ |
FKS1 | Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic sub... | 4 | 4 | 3.3% | 0.00207 | 214849 |
[ |
KEM1 | Evolutionarily-conserved 5'-3' exonuclease component of cytoplasmic processing (P) bodies involved i... | 2 | 2 | 1.9% | 0.00127 | 175459 |