Protein Overview: RPN7

General Information:
Name(s) found:	RPN7 / YPR108W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|914979\|gb\|AAB68078.1\| Ypr108wp [Saccharomyces cerevisiae] [NCBI NR] gi\|6325365\|ref\|NP_015433.1\| Essential, non-ATPase regulatory subunit of the 26S proteasome, similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits; Rpn7p [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S59773 26S proteasome regulatory particle chain RPN7 - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|20978674\|sp\|Q06103.3\|RPN7_YEAST RecName: Full=26S proteasome regulatory subunit RPN7 [NCBI NR] 26S proteasome regulatory subunit RPN7 OS=Saccharomyces cerevisiae GN=RPN7 PE=1 SV=3 [Swiss-Prot] RPN7 Subunit of the regulatory particle of the proteasome [mips] Essential, non-ATPase regulatory subunit of the 26S proteasome, similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits [SGD] (Q06103) 26S proteasome regulatory subunit RPN7 [4932.FASTACCERV.txt] Chr XVI from 742450-743739 [hmr_yeast5_DM3-1_pombe.fasta] Chr XVI from 742451-743740 [yeastorf3.fasta] RPN7, Chr XVI from 742451-743740 [yeast_orfs2] RPN7 SGDID:S0006312, Chr XVI from 742450-743739, Verified ORF [yeastflysequest.fasta] RPN7 SGDID:S0006312, Chr XVI from 742452-743741, Verified ORF [10112005cervSGD.fasta] Essential, non-ATPase regulatory subunit of the 26S proteasome, similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits [Saccharomyces cerevisiae]gi\|914979\|gb\|AAB68078.1\| Ypr108wp [Saccharomyces cerevisiae] [nrdb_11152004_Chlamydomonas] RPN7 SGDID:S000006312, Chr XVI from 742452-743741, Verified ORF, "Essential, non-ATPase regulatory subunit of the 26S proteasome, similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Essential, non-ATPase regulatory subunit of the 26S proteasome, similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits; Rpn7p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Ypr108wp [Saccharomyces cerevisiae] [nr-271106-contam.fasta] 26S proteasome regulatory subunit RPN7 [nr-271106-contam.fasta] [chemostatdb.fasta] 26S proteasome regulatory subunit RPN7 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RPN7 PE=1 SV=3 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] RPN7 SGDID:S000006312, Chr XVI from 742454-743743, Genome Release 64-1-1, Verified ORF, "Essential, non-ATPase regulatory subunit of the 26S proteasome, similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits" [yeast-030211-contam.fasta] RPN7 SGDID:S000006312, Chr XVI from 742454-743743, Genome Release 64-2-1, Verified ORF, "Essential non-ATPase regulatory subunit of the 26S proteasome; similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits" [20141208_orf_trans.fasta] TR5955\|c0_g1_i1\|g.4225 ORF TR5955\|c0_g1_i1\|g.4225 TR5955\|c0_g1_i1\|m.4225 type:complete len:430 (+) TR5955\|c0_g1_i1:93-1382(+) [DBVPG6044_S_cerevisiae-Sep2015-contam.fasta] YPR108W SGDID:S000006312, Chr XVI from 742454-743743, Genome Release 64-1-1, Verified ORF, "Essential, non-ATPase regulatory subunit of the 26S proteasome, similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] ID=asmbl_3287\|g.2237 ORF ID=asmbl_3287\|g.2237 ID=asmbl_3287\|m.2237 type:complete len:430 (+) ID=asmbl_3287:130-1419(+) [DBVPG6044_quiverGenomes_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	429 amino acids
Gene Ontology:
Cellular Component:	proteasome regulatory particle, lid subcomplex [IPI]
Biological Process:	ubiquitin-dependent protein catabolic process [TAS ]
Molecular Function:	structural molecule activity [IMP]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MVDVEEKSQE VEYVDPTVNR VPNYEVSEKA FLLTQSKVSI EQRKEAAEFV LAKIKEEEMA  60
   61 PYYKYLCEEY LVNNGQSDLE HDEKSDSLNE WIKFDQELYN ELCKKNESKI KELNEKIQKL 120
  121 EEDDEGELEQ AQAWINLGEY YAQIGDKDNA EKTLGKSLSK AISTGAKIDV MLTIARLGFF 180
  181 YNDQLYVKEK LEAVNSMIEK GGDWERRNRY KTYYGIHCLA VRNFKEAAKL LVDSLATFTS 240
  241 IELTSYESIA TYASVTGLFT LERTDLKSKV IDSPELLSLI STTAALQSIS SLTISLYASD 300
  301 YASYFPYLLE TYANVLIPCK YLNRHADFFV REMRRKVYAQ LLESYKTLSL KSMASAFGVS 360
  361 VAFLDNDLGK FIPNKQLNCV IDRVNGIVET NRPDNKNAQY HLLVKQGDGL LTKLQKYGAA 420
  421 VRLTGSDRV

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		DBF4 ECM29 NAS6 OSH7 PRE5 RPN1 RPN10 RPN11 RPN12 RPN13 RPN2 RPN3 RPN5 RPN6 RPN7 RPN8 RPN9 RPT1 RPT2 RPT3 RPT4 RPT5 RPT6 SEC18 SEM1 SPG5 UBP6 URA3
View Details	(MIPS) Mewes HW, et al. (2004)		NAS6 RPN1 RPN10 RPN11 RPN12 RPN2 RPN3 RPN5 RPN6 RPN7 RPN8 RPN9 RPT1 RPT2 RPT3 RPT4 RPT5 RPT6
View Details	Krogan NJ, et al. (2006)		CSI1 CSN9 ERV46 FPR2 HUL5 IQG1 MOT1 MPT5 NAS6 NMD3 NPT1 PCI8 PEP1 PST1 PUS9 RPN1 RPN10 RPN11 RPN12 RPN13 RPN14 RPN2 RPN5 RPN6 RPN7 RPN8 RPN9 RPT1 RPT2 RPT3 RPT4 RPT5 RPT6 RRI1 RRI2 SEC18 SEM1 SKP1 SPG5 SPO77 SQT1 SWA2 TCB2 TIP41 TOD6 TOR2 TSR4 UBP6 YEN1 YKL027W YMR258C YNL022C YPR003C YPT52
View Details	Gavin AC, et al. (2006)		ECM29 RPN1 RPN10 RPN11 RPN12 RPN13 RPN5 RPN6 RPN7 RPN8 RPN9 RPT1
View Details	Gavin AC, et al. (2002)		BLM10 CDC6 ECM29 GFA1 MLH2 NAS6 PGK1 PRE1 PRE10 PRE2 PRE3 PRE4 PRE5 PRE6 PRE7 PRE8 PRE9 PUP3 RGR1 RPN10 RPN11 RPN12 RPN13 RPN3 RPN5 RPN6 RPN7 RPN8 RPN9 RPT1 RPT2 RPT3 RPT4 RPT5 RPT6 SCL1 UBP6 YFL006W
View Details	Ho Y, et al. (2002)		AAC1 AAC3 ADE5,7 AHA1 ARC1 ARO1 ARP2 ATG11 ATG12 ATG17 BCK1 BTN2 BUL1 CAR2 CCT2 CCT6 CMD1 CMP2 COP1 CPA2 CPR6 CTR1 DHH1 EAP1 FET4 GAL7 GCD11 GDT1 GFA1 GUS1 IDH1 IDH2 IPP1 LHS1 LYS12 MDN1 MEH1 MKK2 MKT1 NAS6 NPA3 PPX1 PRB1 PTC7 REP1 REX2 RFC3 RGD1 RNQ1 RPN1 RPN10 RPN11 RPN12 RPN13 RPN14 RPN3 RPN5 RPN6 RPN7 RPN8 RPN9 RPT1 RPT2 RPT3 RPT4 RPT5 RVB1 SEC18 SEC27 SEC28 STI1 TCB1 TCP1 TEF4 TIF1, TIF2 TYR1 UBC12 YCR076C YHR020W YHR033W YNL208W
View Details	Qiu et al. (2008)		NAS6 PRE10 PRE6 RPN1 RPN10 RPN11 RPN12 RPN13 RPN2 RPN3 RPN5 RPN6 RPN7 RPN8 RPN9 RPT1 RPT2 RPT3 RPT4 RPT5 RPT6

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)
View Run	ZDS1	Sample 3 - cpn + atp, from june 2005	McCusker D, et al (2007)
View Run	MLP2	#31 Asynchronous Prep (Protease cleavage)	Keck JM, et al. (2011)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#10 Mitotic Prep1-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#11 Mitotic Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	SGT1	RPN7	2	Unpublished Fields Lab Data
View Screen	SGT1	RPN7	2	Unpublished Fields Lab Data
View Screen	SGT1	RPN7	2	Unpublished Fields Lab Data
View Screen	SGT1	RPN7	2	Unpublished Fields Lab Data
View Screen	RPN7	SNA4	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	RPN7	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..90]	MSA	1.165995	View MSA. No confident structure predictions are available.
2	View Details	[91..271]	PSI-BLAST	5.39794	Designed protein cTPR3
3	View Details	[272..429]	MSA	10.215994	View MSA. No confident structure predictions are available.
4	View Details	[319..429]	PSI-BLAST	11.045757	Solution structure of the PCI domain

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.96

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2