Protein Overview: CAM1

General Information:
Name(s) found:	CAM1 / YPL048W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6325209\|ref\|NP_015277.1\| Nuclear protein required for transcription of MXR1; binds the MXR1 promoter in the presence of other nuclear factors; binds calcium and phospholipids; has similarity to translational cofactor EF-1 gamma; Cam1p [Saccharomyces ce [NCBI NR] gi\|5517\|emb\|CAA48116.1\| binding protein homologous to elongation factor 1-gamma [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S29345 translation elongation factor eEF-1 gamma chain homolog CAM1 - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1706589\|sp\|P29547.2\|EF1G1_YEAST RecName: Full=Elongation factor 1-gamma 1; Short=EF-1-gamma 1; AltName: Full=Translation elongation factor 1B gamma 1; AltName: Full=Eukaryotic elongation factor 1Bgamma 1; Short=eEF1Bgamma 1; AltName: Full=Calcium and m [NCBI NR] gi\|1171411\|gb\|AAB68173.1\| Cam1p: Calcium and phospholipid binding protein homologous to translation elongation factor-1 gamma [Saccharomyces cerevisiae] [NCBI NR] (P29547) Elongation factor 1-gamma 1 (EF-1-gamma 1) [Swiss-Prot] Elongation factor 1-gamma 1 OS=Saccharomyces cerevisiae GN=CAM1 PE=1 SV=2 [Swiss-Prot] CAM1 Translation elongation factor eEF1 gamma homologue [mips] Nuclear protein required for transcription of MXR1; binds the MXR1 promoter in the presence of other nuclear factors; binds calcium and phospholipids; has similarity to translational cofactor EF-1 gamma [SGD] Chr XVI from 464396-465643 [hmr_yeast5_DM3-1_pombe.fasta] CAM1, Chr XVI from 464396-465643 [yeast_orfs2] CAM1 SGDID:S0005969, Chr XVI from 464396-465643, Verified ORF [yeastflysequest.fasta] CAM1 SGDID:S0005969, Chr XVI from 464398-465645, Verified ORF [10112005cervSGD.fasta] Calcium and phospholipid binding protein homologous to translation elongation factor 1-gamma (EF-1gamma) [Saccharomyces cerevisiae]gi\|5517\|emb\|CAA48116.1\| binding protein homologous to elongation factor 1-gamma [Saccharomyces cerevisiae]gi\|1171411\|gb\|AA [nrdb_11152004_Chlamydomonas] CAM1 SGDID:S000005969, Chr XVI from 464398-465645, Verified ORF, "Translational cofactor elongation factor-1 gamma, participates in the regulation of GTP-binding protein EF-1 alpha, may play a redundant role in the regulation of protein synthesis or anoth [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] CAM1 SGDID:S000005969, Chr XVI from 464398-465645, Verified ORF, "Homolog of translational cofactor elongation factor-1 gamma, participates in the regulation of GTP-binding protein EF-1 alpha, may play a redundant role in the regulation of protein synthes [yeast-contam.fasta] CAM1 SGDID:S000005969, Chr XVI from 464398-465645, Verified ORF, "Nuclear protein required for transcription of MXR1; binds the MXR1 promoter in the presence of other nuclear factors; binds calcium and phospholipids; has similarity to translational cofact [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] binding protein homologous to elongation factor 1-gamma [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Elongation factor 1-gamma 1 (EF-1-gamma 1) (Translation elongation factor 1B gamma 1) (Eukaryotic elongation factor 1Bgamma 1) (eEF1Bgamma 1) (Calcium and membrane-binding protein 1) (Calcium phospholipid-binding protein) (CPBP) [nr-271106-contam.fasta] Cam1p: Calcium and phospholipid binding protein homologous to translation elongation factor-1 gamma [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Homolog of translational cofactor elongation factor-1 gamma, participates in the regulation of GTP-binding protein EF-1 alpha, may play a redundant role in the regulation of protein synthesis or another GTP-dependent process; Cam1p [Saccharomyces cerevisi [nr-271106-contam.fasta] CAM1 SGDID:S000005969, Chr XVI from 464398-465645, Verified ORF, "Nuclear protein required for transcription of MXR1; binds the MXR1 promoter in the presence of other nuclear factors; binds calcium and phospholipids; has similarity to translational cofactor EF-1 gamma" [yeast-bovrabprots.fasta] CAM1 SGDID:S000005969, Chr XVI from 464398-465645, Verified ORF, "Homolog of translational cofactor elongation factor-1 gamma, participates in the regulation of GTP-binding protein EF-1 alpha, may play a redundant role in the regulation of protein synthesis or another GTP-dependent process" [yeast-contam_custom.fasta] [chemostatdb.fasta] Elongation factor 1-gamma 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=CAM1 PE=1 SV=2 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] CAM1 SGDID:S000005969, Chr XVI from 464401-465648, Genome Release 64-1-1, Verified ORF, "Nuclear protein required for transcription of MXR1; binds the MXR1 promoter in the presence of other nuclear factors; binds calcium and phospholipids; has similarity to translational cofactor EF-1 gamma" [yeast-030211-contam.fasta] CAM1 SGDID:S000005969, Chr XVI from 464401-465648, Genome Release 64-2-1, Verified ORF, "Nuclear protein required for transcription of MXR1; binds the MXR1 promoter in the presence of other nuclear factors; binds calcium and phospholipids; has similarity to translational cofactor EF-1 gamma" [20141208_orf_trans.fasta] TR3250\|c0_g1_i1\|g.2372 ORF TR3250\|c0_g1_i1\|g.2372 TR3250\|c0_g1_i1\|m.2372 type:complete len:416 (+) TR3250\|c0_g1_i1:122-1369(+) [Y12_S_cerevisiae-Sep2015-contam.fasta] YPL048W SGDID:S000005969, Chr XVI from 464401-465648, Genome Release 64-1-1, Verified ORF, "Nuclear protein required for transcription of MXR1; binds the MXR1 promoter in the presence of other nuclear factors; binds calcium and phospholipids; has similarity to translational cofactor EF-1 gamma" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] asmbl_3258\|g.2436 ORF asmbl_3258\|g.2436 asmbl_3258\|m.2436 type:complete len:416 (+) asmbl_3258:123-1370(+) [Y12_Trinity_v2_2_0_PASA_6May2016-contam.fasta] ID=asmbl_3259\|g.2075 ORF ID=asmbl_3259\|g.2075 ID=asmbl_3259\|m.2075 type:complete len:416 (+) ID=asmbl_3259:123-1370(+) [Y12_quiver_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	415 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA][IEA] cytoplasm [IEA] eukaryotic translation elongation factor 1 complex [IEA]
Biological Process:	regulation of translational elongation [IGI][ISS]
Molecular Function:	translation elongation factor activity [ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSQGTLYANF RIRTWVPRGL VKALKLDVKV VTPDAAAEQF ARDFPLKKVP AFVGPKGYKL  60
   61 TEAMAINYYL VKLSQDDKMK TQLLGADDDL NAQAQIIRWQ SLANSDLCIQ IANTIVPLKG 120
  121 GAPYNKKSVD SAMDAVDKIV DIFENRLKNY TYLATENISL ADLVAASIFT RYFESLFGTE 180
  181 WRAQHPAIVR WFNTVRASPF LKDEYKDFKF ADKPLSPPQK KKEKKAPAAA PAASKKKEEA 240
  241 KPAATETETS SKKPKHPLEL LGKSTFVLDD WKRKYSNEDT RPVALPWFWE HYNPEEYSLW 300
  301 KVTYKYNDEL TLTFMSNNLV GGFFNRLSAS TKYMFGCLVV YGENNNNGIV GAVMVRGQDY 360
  361 VPAFDVAPDW ESYDYAKLDP TNDDDKEFIN NMWAWDKPVS VNGEPKEIVD GKVLK

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		CAM1 EFB1 FBA1 HSC82 HSP104 RPS0A SSZ1 TEF4 YEF3 ZUO1
View Details	(MIPS) Mewes HW, et al. (2004)		CAM1 EFB1 HBS1 TEF1, TEF2 TEF4
View Details	Krogan NJ, et al. (2006)		ARO4 CAM1 TEF4 TMA19
View Details	Gavin AC, et al. (2006)		CAM1 EFB1 HSL1 RAD34 TEF1, TEF2
View Details	Gavin AC, et al. (2002)		CAM1 EFB1 FAP1 GFA1 GUS1 ILV1 KAP123 MGA2 NEW1 OLA1 SBP1 SEC27 TEF1, TEF2 TEF4
View Details	Qiu et al. (2008)		CAM1 TEF1, TEF2 TEF4 TIF5

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)
View Run	ZDS1	Sample 2- cpn2 from june 2005	McCusker D, et al (2007)
View Run	ZDS1	Sample 3 - cpn + atp, from june 2005	McCusker D, et al (2007)
View Run	BOI1	Sample boi 1 with ha tag from october 2005	McCusker D, et al (2007)
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	GPA1	CAM1	1	Unpublished Fields Lab Data
View Screen	SPT21	CAM1	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	CAM1	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..223]	PSI-BLAST	40.69897	GST-like domain of elongation factor 1-gamma
2	View Details	[224..415]	PSI-BLAST	77.0	Elongation factor 1-gamma C-terminal domain

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
transcription regulator activity	2.30799114442278	bayes_pls_golite062009
nucleic acid binding	2.06381591939564	bayes_pls_golite062009
DNA binding	1.92118545500784	bayes_pls_golite062009
binding	1.25802922431343	bayes_pls_golite062009
transcription activator activity	1.23146660729357	bayes_pls_golite062009
protein binding	1.18479617596618	bayes_pls_golite062009
catalytic activity	0.672192331361968	bayes_pls_golite062009
RNA binding	0.382017633056715	bayes_pls_golite062009
	0.359224524631956	bayes_pls_golite062009
receptor binding	0.0497559408754589	bayes_pls_golite062009

Term	Confidence	Notes
translation elongation factor activity	11.9602302019768	bayes_pls_golite062009
translation factor activity, nucleic acid binding	7.4644014407268	bayes_pls_golite062009
translation regulator activity	7.42485540728491	bayes_pls_golite062009
nucleic acid binding	3.9329721501879	bayes_pls_golite062009
binding	3.0325775548747	bayes_pls_golite062009
protein binding	1.65598815428763	bayes_pls_golite062009

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2