Protein Overview: INP52

General Information:
Name(s) found:	INP52 / YNL106C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6324224\|ref\|NP_014293.1\| Polyphosphatidylinositol phosphatase, dephosphorylates a number of phosphatidylinositols (PIs) to PI; involved in endocytosis; hyperosmotic stress causes translocation to actin patches; synaptojanin-like protein with a Sac1 dom [NCBI NR] pir\|\|S63046 probable membrane protein YNL106c - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1730760\|sp\|P50942.1\|INP52_YEAST RecName: Full=Inositol-1,4,5-trisphosphate 5-phosphatase 2; AltName: Full=Synaptojanin-like protein 2 [NCBI NR] gi\|1302022\|emb\|CAA95982.1\| PIE3 [Saccharomyces cerevisiae] [NCBI NR] Uncharacterized protein YNL106C OS=Saccharomyces cerevisiae GN=YNL106C PE=1 SV=1 [Swiss-Prot] Inositol-1,4,5-trisphosphate 5-phosphatase 2 OS=Saccharomyces cerevisiae GN=INP52 PE=1 SV=1 [Swiss-Prot] INP52 Phosphatidylinositol phosphate phosphatase [mips] Polyphosphatidylinositol phosphatase, dephosphorylates a number of phosphatidylinositols (PIs) to PI; involved in endocytosis; hyperosmotic stress causes translocation to actin patches; synaptojanin-like protein with a Sac1 domain [SGD] INP52, Chr XIV from 420941-424492, reverse complement [yeast_orfs2] Chr XIV from 420941-424492, reverse complement [yeastorf3.fasta] Chr XIV from 424492-420941, reverse complement [yeastorf5.fasta] (P50942) Hypothetical 133.3 kDa protein in CYB5-LEU4 intergenic region [4932.FASTACCERV.txt] INP52 SGDID:S0005050, Chr XIV from 424495-420944, reverse complement, Verified ORF [yeastflysequest.fasta] Inp52p [Saccharomyces cerevisiae]gi\|1302022\|emb\|CAA95982.1\| PIE3 [Saccharomyces cerevisiae]gi\|2132747\|pir\|\|S63046 probable membrane protein YNL106c - yeast (Saccharomyces cerevisiae)gi\|1730760\|sp\|P50942\|YNK6_YEAST Hypothetical 133.3 kDa protein in CYB5 [nrdb_11152004_Chlamydomonas] INP52 SGDID:S000005050, Chr XIV from 424497-420946, reverse complement, Verified ORF, "Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in endocytosis; hyperosmotic stress causes t [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] INP52 SGDID:S000005050, Chr XIV from 424497-420946, reverse complement, Verified ORF, "Polyphosphatidylinositol phosphatase, dephosphorylates a number of phosphatidylinositols (PIs) to PI; involved in endocytosis; hyperosmotic stress causes translocation [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] Uncharacterized protein YNL106C [nr-271106-contam.fasta] Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in endocytosis; hyperosmotic stress causes translocation to actin patches; Inp52p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] PIE3 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] INP52 SGDID:S000005050, Chr XIV from 424497-420946, reverse complement, Verified ORF, "Polyphosphatidylinositol phosphatase, dephosphorylates a number of phosphatidylinositols (PIs) to PI; involved in endocytosis; hyperosmotic stress causes translocation to actin patches; synaptojanin-like protein with a Sac1 domain" [yeast-bovrabprots.fasta] INP52 SGDID:S000005050, Chr XIV from 424497-420946, reverse complement, Verified ORF, "Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in endocytosis; hyperosmotic stress causes translocation to actin patches" [yeast-contam_custom.fasta] [chemostatdb.fasta] Polyphosphatidylinositol phosphatase INP52 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=INP52 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] INP52 SGDID:S000005050, Chr XIV from 424495-420944, Genome Release 64-1-1, reverse complement, Verified ORF, "Polyphosphatidylinositol phosphatase, dephosphorylates a number of phosphatidylinositols (PIs) to PI; involved in endocytosis; hyperosmotic stress causes translocation to actin patches; synaptojanin-like protein with a Sac1 domain" [yeast-030211-contam.fasta] INP52 SGDID:S000005050, Chr XIV from 424495-420944, Genome Release 64-2-1, reverse complement, Verified ORF, "Polyphosphatidylinositol phosphatase; dephosphorylates a number of phosphatidylinositol phosphates (PtdInsPs, PIPs) to PI; involved in endocytosis; hyperosmotic stress causes translocation to actin patches; synaptojanin-like protein with a Sac1 domain; INP52 has a paralog, INP53, that arose from the whole genome duplication" [20141208_orf_trans.fasta] YNL106C SGDID:S000005050, Chr XIV from 424495-420944, Genome Release 64-1-1, reverse complement, Verified ORF, "Polyphosphatidylinositol phosphatase, dephosphorylates a number of phosphatidylinositols (PIs) to PI; involved in endocytosis; hyperosmotic stress causes translocation to actin patches; synaptojanin-like protein with a Sac1 domain" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	1183 amino acids
Gene Ontology:
Cellular Component:	membrane fraction [IDA] actin cortical patch [IDA ]
Biological Process:	cellular cell wall organization [IGI] inositol lipid-mediated signaling [TAS] endocytosis [IGI] phosphoinositide dephosphorylation [ISS]
Molecular Function:	phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity [IDA] [NOT]inositol-polyphosphate 5-phosphatase activity [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MKILLSKQQT RKIAIVSETH GLVFRPINSK NSRRSTCAVE LVPKAELNGN GFRRLSNHEI  60
   61 YGFIGLIEIE GLMFIATITG KSKVAQPIPN KTVNKIYAVD FFCLNNSKWD FMDIDSSGYP 120
  121 IVTNDGDFAI SSPPSISTHS SRSSLRSSSS RSLNAQEQAP KHPCHELRKL LSNGSFYYST 180
  181 DFDLTCTLQK RGFTEHSLSF DDFDREFMWN SFLMDEIITY RDRLDVTAKE LLDQRGFLTT 240
  241 VIRGFAETIF SYINRLKVGL TIISRQSWKR AGTRFNARGI DDDGHVANFV ETEMIMYSSQ 300
  301 YCYAFTQIRG SLPIFWEQDT SLISPKIQIT RSVEATQPTF DEHFIRLFKK YGPVHIINLL 360
  361 STKSSEIQLS RRYKEQLKNS EKMKIGRDVF LTSFDFHRET SQDGFAAASR IIPKIRNTIL 420
  421 DAGYFSYDVK EGRLISEQDG VFRTNCLDCL DRTNLIQQTI SLAVFKLFLE DFRLVKPSSF 480
  481 IDDNEFVQKV NALWADNGDQ ISQIYTGTNA LKSSYSRKGK MSFSGALSDA TKSVSRMYIN 540
  541 NFVDKGKQQN IDTLLGKLPH QQVVELYDPI CEYVNERLLE SEEKFTTHSN INLFVGTFNV 600
  601 NGNSRRADLS KWLFPIGDKF KPDVVVLGLQ EVIELTAGSI LNADYTKSSF WETMVTDCLN 660
  661 QYEEKYLLLR VEQMSSLLIL FFARSDRAYN IKEVGGSTKK TGFGGITGNK GAVAIRFDYG 720
  721 ATSFCFVNTH LSAGASNIDE RRNDYNNIYR NITFPRSKTI PHHDSLFWLG DLNYRITLTN 780
  781 DEVRRELRAQ KDGYIDRLLQ YDQLTQEINE GVVFQGFKEP TLQFRPTYKY DYGTDNYDTS 840
  841 EKARTPSWTD RIIYKGENLH PLAYSDAPLK ISDHKPVYAA YRANVKFVDE KEKLNLVEKL 900
  901 YAEYKNTHPE ALTTGPDELS HARMEKQKES IPLDATVQSA GIKLIDLDDT SSCVSPLLSG 960
  961 PSPQPSVVGP GGLSNVSPDK SKLNVLPPPP PTSRHNKEPS SKLLSPTKEI SIVSVSPRKG1020
 1021 ESNLPALERH STPKPLPPVP ALSLSKPVSL QKSSSELQHA KETIDNGKIV PRPCPPIRRK1080
 1081 SSTAPDEIST STKNSGVSTT EDPEPAKAST KPEKPPVVKK PHYLSVAANK LNTSQEHSIK1140
 1141 VSPSNSKSEE ELPCKKKSKP KVPAKNPELE KLSVHPLKPC DPN

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		ABP1 BSP1 CAP1 CMD1 CRN1 INP52 LSP1 MLC1 NOP53 PIL1 RVS161 SLM1
View Details	Krogan NJ, et al. (2006)		ARC15 ASR1 CAJ1 CDC7 CRN1 INP52 IRC22 MSK1 PNO1 PST2 RAD34 SAC6 SAR1 SLM1 TCP1 TED1 YPP1
View Details	Gavin AC, et al. (2002)		BSP1 BZZ1 CHC1 ECM25 END3 INP52 LAS17 LSB3 RAD51 SLA1 SLA2 STM1 SYP1 TFP1 VRP1 YPT7 YSC84
View Details	Ho Y, et al. (2002)		ADO1 INP52 POR1 RNH202 RNQ1 SAP190 TIF1, TIF2 YHB1
View Details	Qiu et al. (2008)		INP51 INP52 INP53

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	BOI1	Sample boi1 with gst from october 2005	McCusker D, et al (2007)
View Run	MLP2	#26 Asynchronous Prep4-TiO2 Phosphopeptide enriched, Steps1-2	Keck JM, et al. (2011)
View Run	MLP2	#03 Alpha Factor Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	INP52	PTP1	2	Unpublished Fields Lab Data
View Screen	INP52	SVL3	2	Unpublished Fields Lab Data
View Screen	INP52	VPS4	2	Unpublished Fields Lab Data
View Screen	INP52	YDR026C	2	Unpublished Fields Lab Data
View Screen	INP52	MLC2	2	Unpublished Fields Lab Data
View Screen	INP52	RTT105	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	INP52	Huh WK, et al. (2003)
View Data	INP52 and SAC6	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..475]	Pfam	183.337242	SacI homology domain No confident structure predictions are available.
2	View Details	[476..559]	deduced	N/A	Confident ab initio structure predictions are available.
3	View Details	[560..938]	PSI-BLAST	904.9897	Synaptojanin, IPP5C domain
4	View Details	[939..1183]	ab initio	11.26	No description for 1deqA was found.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.77

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2