Protein Overview: DSK2

General Information:
Name(s) found:	DSK2 / YMR276W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|825542\|emb\|CAA89774.1\| unknown [Saccharomyces cerevisiae] [NCBI NR] gi\|6323932\|ref\|NP_014003.1\| Nuclear-enriched ubiquitin-like polyubiquitin-binding protein, required for spindle pole body (SPB) duplication and for transit through the G2/M phase of the cell cycle, involved in proteolysis, interacts with the proteasome; D [NCBI NR] pir\|\|S54583 ubiquitin-like protein DSK2 - yeast (Saccharomyces cerevisiae) [NCBI NR] DSK2 Ubiquitin-like protein [mips] Nuclear-enriched ubiquitin-like polyubiquitin-binding protein, required for spindle pole body (SPB) duplication and for transit through the G2/M phase of the cell cycle, involved in proteolysis, interacts with the proteasome [SGD] DSK2, Chr XIII from 818827-819948 [yeast_orfs2] Chr XIII from 818827-819948 [yeastorf3.fasta] DSK2 SGDID:S0004889, Chr XIII from 818826-819947, Verified ORF [yeastflysequest.fasta] Dsk2p [Saccharomyces cerevisiae]gi\|825542\|emb\|CAA89774.1\| unknown [Saccharomyces cerevisiae]gi\|1078532\|pir\|\|S54583 ubiquitin-like protein DSK2 - yeast (Saccharomyces cerevisiae) [nrdb_11152004_Chlamydomonas] DSK2 SGDID:S000004889, Chr XIII from 818826-819947, Verified ORF, "Nuclear-enriched ubiquitin-like polyubiquitin-binding protein, required for spindle pole body (SPB) duplication and for transit through the G2/M phase of the cell cycle, involved in proteo [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Nuclear-enriched ubiquitin-like polyubiquitin-binding protein, required for spindle pole body (SPB) duplication and for transit through the G2/M phase of the cell cycle, involved in proteolysis, interacts with the proteasome; Dsk2p [Saccharomyces cerevisi [nr-271106-contam.fasta] unknown [Saccharomyces cerevisiae] [nr-271106-contam.fasta] DSK2 SGDID:S000004889, Chr XIII from 818826-819947, Verified ORF, "Nuclear-enriched ubiquitin-like polyubiquitin-binding protein, required for spindle pole body (SPB) duplication and for transit through the G2/M phase of the cell cycle, involved in proteolysis, interacts with the proteasome" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Ubiquitin domain-containing protein DSK2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=DSK2 PE=1 SV=2 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] DSK2 SGDID:S000004889, Chr XIII from 818827-819948, Genome Release 64-1-1, Verified ORF, "Nuclear-enriched ubiquitin-like polyubiquitin-binding protein, required for spindle pole body (SPB) duplication and for transit through the G2/M phase of the cell cycle, involved in proteolysis, interacts with the proteasome" [yeast-030211-contam.fasta] DSK2 SGDID:S000004889, Chr XIII from 818827-819948, Genome Release 64-2-1, Verified ORF, "Nuclear-enriched ubiquitin-like polyubiquitin-binding protein; required for spindle pole body (SPB) duplication and for transit through the G2/M phase of the cell cycle; involved in proteolysis; interacts with the proteasome; protein abundance increases in response to DNA replication stress" [20141208_orf_trans.fasta] YMR276W SGDID:S000004889, Chr XIII from 818827-819948, Genome Release 64-1-1, Verified ORF, "Nuclear-enriched ubiquitin-like polyubiquitin-binding protein, required for spindle pole body (SPB) duplication and for transit through the G2/M phase of the cell cycle, involved in proteolysis, interacts with the proteasome" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	373 amino acids
Gene Ontology:
Cellular Component:	nucleus [TAS]
Biological Process:	spindle pole body duplication in nuclear envelope [TAS] ER-associated protein catabolic process [IMP]
Molecular Function:	protein binding, bridging [IMP][IPI]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSLNIHIKSG QDKWEVNVAP ESTVLQFKEA INKANGIPVA NQRLIYSGKI LKDDQTVESY  60
   61 HIQDGHSVHL VKSQPKPQTA SAAGANNATA TGAAAGTGAT PNMSSGQSAG FNPLADLTSA 120
  121 RYAGYLNMPS ADMFGPDGGA LNNDSNNQDE LLRMMENPIF QSQMNEMLSN PQMLDFMIQS 180
  181 NPQLQAMGPQ ARQMLQSPMF RQMLTNPDMI RQSMQFARMM DPNAGMGSAG GAASAFPAPG 240
  241 GDAPEEGSNT NTTSSSNTGN NAGTNAGTNA GANTAANPFA SLLNPALNPF ANAGNAASTG 300
  301 MPAFDPALLA SMFQPPVQAS QAEDTRPPEE RYEHQLRQLN DMGFFDFDRN VAALRRSGGS 360
  361 VQGALDSLLN GDV

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Krogan NJ, et al. (2006)		DSK2 LYP1 PDH1
View Details	Ho Y, et al. (2002)		DSK2 HMF1 NPL4 RAD23 SHP1 TSL1 UBI4 UFD2 YDR049W

Mass Spectrometry Data

No mass spectrometry results found for this protein.

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	SRC1	DSK2	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	DSK2	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..107]	PSI-BLAST	110.703922	Rub1
2	View Details	[108..259]	MSA	2.096982	View MSA. No confident structure predictions are available.
3	View Details	[260..329]	deduced	N/A	No confident structure predictions are available.
4	View Details	[330..373]	PSI-BLAST	11.30103	DNA repair protein Hhr23a

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
binding	3.35791544110308	bayes_pls_golite062009
small conjugating protein ligase activity	1.83465130904364	bayes_pls_golite062009
protein binding	1.76858817153382	bayes_pls_golite062009
transcription regulator activity	1.42747465816672	bayes_pls_golite062009
nucleic acid binding	1.32707653701567	bayes_pls_golite062009
DNA binding	1.22622447288034	bayes_pls_golite062009
ubiquitin-protein ligase activity	1.16468510691469	bayes_pls_golite062009
tubulin binding	1.11505425062041	bayes_pls_golite062009
acid-amino acid ligase activity	0.919595189902044	bayes_pls_golite062009
cytoskeletal protein binding	0.907853439770547	bayes_pls_golite062009
microtubule binding	0.84012258724586	bayes_pls_golite062009
hydrolase activity	0.247201449341942	bayes_pls_golite062009

Term	Confidence	Notes
binding	2.9974054448593	bayes_pls_golite062009
DNA binding	2.7109101781315	bayes_pls_golite062009
nucleic acid binding	2.65801759476965	bayes_pls_golite062009
damaged DNA binding	2.33590042963509	bayes_pls_golite062009
transcription regulator activity	1.57741426576729	bayes_pls_golite062009
ubiquitin binding	1.38611607035372	bayes_pls_golite062009
small conjugating protein binding	1.25128759629714	bayes_pls_golite062009
protein binding	0.989474580552798	bayes_pls_golite062009
hydrolase activity	0.365329242181237	bayes_pls_golite062009
catalytic activity	0.2736776367506	bayes_pls_golite062009
peptidase activity	0.0113950255895392	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.96

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2