Protein Overview: IFH1

General Information:
Name(s) found:	IFH1 / YLR223C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|730619\|sp\|P39520.1\|IFH1_YEAST RecName: Full=Protein IFH1; AltName: Full=Ribosomal RNA-processing protein 3 [NCBI NR] gi\|6323252\|ref\|NP_013324.1\| Essential protein with a highly acidic N-terminal domain; IFH1 exhibits genetic interactions with FHL1, overexpression interferes with silencing at telomeres and HM loci; potential Cdc28p substrate; Ifh1p [Saccharomyces cerevis [NCBI NR] gi\|609372\|gb\|AAB67412.1\| Ifh1p [Saccharomyces cerevisiae] [NCBI NR] gi\|531492\|emb\|CAA82624.1\| RRP3p [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S55352 IFH1 protein - yeast (Saccharomyces cerevisiae) [NCBI NR] Protein IFH1 OS=Saccharomyces cerevisiae GN=IFH1 PE=1 SV=1 [Swiss-Prot] IFH1 Pre-rRNA processing machinery control protein [mips] Coactivator that regulates transcription of ribosomal protein (RP) genes; recruited to RP gene promoters during optimal growth conditions via Fhl1p; subunit of CURI, a complex that coordinates RP production and pre-rRNA processing [SGD] IFH1, Chr XII from 582233-585490, reverse complement [yeast_orfs2] Chr XII from 582233-585490, reverse complement [yeastorf3.fasta] Chr XII from 585490-582233, reverse complement [yeastorf5.fasta] (P39520) IFH1 protein (RRP3 protein) [4932.FASTACCERV.txt] IFH1 SGDID:S0004213, Chr XII from 585492-582235, reverse complement, Verified ORF [yeastflysequest.fasta] Essential protein with a highly acidic N-terminal domain; IFH1 exhibits genetic interactions with FHL1, overexpression interferes with silencing at telomeres and HM loci; potential Cdc28p substrate [Saccharomyces cerevisiae]gi\|531492\|emb\|CAA82624.1\| RRP3 [nrdb_11152004_Chlamydomonas] IFH1 SGDID:S000004213, Chr XII from 585492-582235, reverse complement, Verified ORF, "Essential protein with a highly acidic N-terminal domain; IFH1 exhibits genetic interactions with FHL1, overexpression interferes with silencing at telomeres and HM loci [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] RRP3p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Essential protein with a highly acidic N-terminal domain; IFH1 exhibits genetic interactions with FHL1, overexpression interferes with silencing at telomeres and HM loci; potential Cdc28p substrate; Ifh1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Ifh1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Protein IFH1 (Protein RRP3) [nr-271106-contam.fasta] IFH1 SGDID:S000004213, Chr XII from 585492-582235, reverse complement, Verified ORF, "Essential protein with a highly acidic N-terminal domain; IFH1 exhibits genetic interactions with FHL1, overexpression interferes with silencing at telomeres and HM loci; potential Cdc28p substrate" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Protein IFH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=IFH1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] IFH1 SGDID:S000004213, Chr XII from 585490-582233, Genome Release 64-1-1, reverse complement, Verified ORF, "Coactivator that regulates transcription of ribosomal protein (RP) genes; recruited to RP gene promoters during optimal growth conditions via Fhl1p; subunit of CURI, a complex that coordinates RP production and pre-rRNA processing" [yeast-030211-contam.fasta] IFH1 SGDID:S000004213, Chr XII from 585490-582233, Genome Release 64-2-1, reverse complement, Verified ORF, "Coactivator, regulates transcription of ribosomal protein (RP) genes; recruited to RP gene promoters during optimal growth conditions via Fhl1p; subunit of CURI, a complex that coordinates RP production and pre-rRNA processing; regulated by acetylation and phosphorylation at different growth states via TORC1 signaling; IFH1 has a paralog, CRF1, that arose from the whole genome duplication" [20141208_orf_trans.fasta] YLR223C SGDID:S000004213, Chr XII from 585490-582233, Genome Release 64-1-1, reverse complement, Verified ORF, "Coactivator that regulates transcription of ribosomal protein (RP) genes; recruited to RP gene promoters during optimal growth conditions via Fhl1p; subunit of CURI, a complex that coordinates RP production and pre-rRNA processing" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	1085 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA ] nucleolus [IDA]
Biological Process:	transcription from RNA polymerase III promoter [IGI][IPI] transcription from RNA polymerase II promoter [IMP] chromatin silencing at telomere [IMP]
Molecular Function:	transcription factor activity [IGI][IPI] specific RNA polymerase II transcription factor activity [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MAGKKSPRKS TINHSTHSGK LPANIKRLIK KGESDTKSRQ SPPTLSTTRP RRFSLIYSSE  60
   61 SSLSDVSDSD KNKSTNPHKI KRKAKNISNN SQGKKSKLIQ RQIDNDDEGT ESSDYQAVTD 120
  121 GEESENEEEE SEEEEEDDDE DDDDDDDDGS DSDSDSETSS DDENIDFVKL TAQRKKRAMK 180
  181 ALSAMNTNSN TLYSSRENSN KNKSVKLSPK KENEEEQKEE KEKEKEEQQK QQESNKKEVN 240
  241 GSGTTTTQQA LSFKFKKEDD GISFGNGNEG YNEDIGEEVL DLKNKENNGN EEDKLDSKVM 300
  301 LGNNDELRFP NISESDESEY DIDQDAYFDV INNEDSHGEI GTDLETGEDD LPILEEEEQN 360
  361 IVSELQNDDE LSFDGSIHEE GSDPVEDAEN KFLQNEYNQE NGYDEEDDEE DEIMSDFDMP 420
  421 FYEDPKFANL YYYGDGSEPK LSLSTSLPLM LNDEKLSKLK KKEAKKREQE ERKQRRKLYK 480
  481 KTQKPSTRTT SNVDNDEYIF NVFFQSDDEN SGHKSKKGRH KSGKSHIEHK NKGSNLIKSN 540
  541 DDLEPSTHST VLNSGKYDSS DDEYDNILLD VAHMPSDDEC SESETSHDAD TDEELRALDS 600
  601 DSLDIGTELD DDYEDDDDDS SVTNVFIDID DLDPDSFYFH YDSDGSSSLI SSNSDKENSD 660
  661 GSKDCKHDLL ETVVYVDDES TDEDDNLPPP SSRSKNIGSK AKEIVSSNVV GLRPPKLGTW 720
  721 ETDNKPFSII DGLSTKSLYA LIQEHQQLRE QHQRAQTPDV KREGSSNGNN GDELTLNELL 780
  781 NMSELEDDSP SHTDDMENNY NDAINSKSTN GHAADWYEVP KVPLSAFRNK GINAYEEDEY 840
  841 MIPANSNRKV PIGYIGNERT RKKIDKMKEL QRKKTEKKRQ LKKKKKLLKI RKQRQKAIKE 900
  901 QETMNLQLGI NGHEIIGNNN SHSDINTGTD FTTNENTPMN ELPSHAPEDA SLIPHNSDLA 960
  961 VDSNTRKNST KSVGLDEIHE ILGKDENDLL SVGDINGYDA QEGHVIEDTD ADILASLTAP1020
 1021 VQFDNTLSHE NSNSMWRRRQ SMVEAAAENL RFTKNGLFSE SALADIEGIM GNDVNHSFEF1080
 1081 NDVLQ

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		IFH1 RRP7 UTP22
View Details	(MIPS) Mewes HW, et al. (2004)		DRS1 GAR1 IFH1 NOP1 NPL3 NSR1 POP1 POP2 RAT1 RNT1 RRP3 SBP1 SNM1 SRD1 UTP10 UTP11 UTP13 UTP4 UTP5 UTP6 UTP7 UTP8 UTP9 YME2
View Details	Krogan NJ, et al. (2006)		DHR2 IFH1 RRP7 TDA7 UBP10 UTP22 YGR251W
View Details	Qiu et al. (2008)		DBP7 DRS1 FHL1 GAR1 HAS1 IFH1 NOP4 NPL3 NSR1 POP1 UTP15 UTP7 UTP9

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	ZDS1	Sample 2- cpn2 from june 2005	McCusker D, et al (2007)
View Run	MLP2	#14 Mitotic Prep2-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#30 Asynchronous Prep6-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#06 Alpha Factor Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	STE50	IFH1	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	IFH1	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..406]	PSI-BLAST	27.045757	Heavy meromyosin subfragment
2	View Details	[407..756]	MSA	7.437997	View MSA. No confident structure predictions are available.
3	View Details	[757..1085]	deduced	N/A	No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2