Protein Overview: PAP1

General Information:
Name(s) found:	PAP1 / YKR002W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6322854\|ref\|NP_012927.1\| Poly(A) polymerase, one of three factors required for mRNA 3'-end polyadenylation, forms multiprotein complex with polyadenylation factor I (PF I), also required for mRNA nuclear export; may also polyadenylate rRNAs; Pap1p [Sac [NCBI NR] gi\|486407\|emb\|CAA82072.1\| PAP1 [Saccharomyces cerevisiae] [NCBI NR] gi\|4099\|emb\|CAA42852.1\| poly(A)polymerase [Saccharomyces cerevisiae] [NCBI NR] gi\|3527\|emb\|CAA46250.1\| polyA polymerase 1 [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S19031 poly(A) polymerase - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|266739\|sp\|P29468.1\|PAP_YEAST RecName: Full=Poly(A) polymerase; Short=PAP; AltName: Full=Polynucleotide adenylyltransferase [NCBI NR] gi\|228305\|prf\|\|1802393A poly(A) polymerase [NCBI NR] Poly(A) polymerase OS=Saccharomyces cerevisiae GN=PAP1 PE=1 SV=1 [Swiss-Prot] PAP1 Poly(A) polymerase [mips] Poly(A) polymerase, one of three factors required for mRNA 3'-end polyadenylation, forms multiprotein complex with polyadenylation factor I (PF I), also required for mRNA nuclear export; may also polyadenylate rRNAs [SGD] PAP1, Chr XI from 442870-444576 [yeast_orfs2] Chr XI from 442870-444576 [yeastorf3.fasta] (P29468) Poly(A) polymerase (EC 2.7.7.19) (PAP) (Polynucleotide adenylyltransferase) [4932.FASTACCERV.txt] PAP1 SGDID:S0001710, Chr XI from 442875-444581, Verified ORF [yeastflysequest.fasta] Pap1p [Saccharomyces cerevisiae]gi\|4099\|emb\|CAA42852.1\| poly(A)polymerase [Saccharomyces cerevisiae]gi\|486407\|emb\|CAA82072.1\| PAP1 [Saccharomyces cerevisiae]gi\|3527\|emb\|CAA46250.1\| polyA polymerase 1 [Saccharomyces cerevisiae]gi\|320814\|pir\|\|S19031 pol [nrdb_11152004_Chlamydomonas] PAP1 SGDID:S000001710, Chr XI from 442875-444581, Verified ORF, "Poly(A) polymerase, one of three factors required for mRNA 3'-end polyadenylation, forms multiprotein complex with polyadenylation factor I (PF I), also required for mRNA nuclear export; may [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] poly(A) polymerase [nr-271106-contam.fasta] polyA polymerase 1 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] poly(A)polymerase [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Poly(A) polymerase (PAP) (Polynucleotide adenylyltransferase) [nr-271106-contam.fasta] PAP1 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Poly(A) polymerase, one of three factors required for mRNA 3'-end polyadenylation, forms multiprotein complex with polyadenylation factor I (PF I), also required for mRNA nuclear export; may also polyadenylate rRNAs; Pap1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] PAP1 SGDID:S000001710, Chr XI from 442875-444581, Verified ORF, "Poly(A) polymerase, one of three factors required for mRNA 3'-end polyadenylation, forms multiprotein complex with polyadenylation factor I (PF I), also required for mRNA nuclear export; may also polyadenylate rRNAs" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Poly(A) polymerase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PAP1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] PAP1 SGDID:S000001710, Chr XI from 443232-444938, Genome Release 64-1-1, Verified ORF, "Poly(A) polymerase, one of three factors required for mRNA 3'-end polyadenylation, forms multiprotein complex with polyadenylation factor I (PF I), also required for mRNA nuclear export; may also polyadenylate rRNAs; required for gene looping" [yeast-030211-contam.fasta] PAP1 SGDID:S000001710, Chr XI from 443232-444938, Genome Release 64-2-1, Verified ORF, "Poly(A) polymerase; one of three factors required for mRNA 3'-end polyadenylation, forms multiprotein complex with polyadenylation factor I (PF I), also required for mRNA nuclear export; may also polyadenylate rRNAs; required for gene looping" [20141208_orf_trans.fasta] TR1043\|c0_g1_i1\|g.816 ORF TR1043\|c0_g1_i1\|g.816 TR1043\|c0_g1_i1\|m.816 type:complete len:569 (+) TR1043\|c0_g1_i1:261-1967(+) [Y12_S_cerevisiae-Sep2015-contam.fasta] TR6887\|c0_g1_i1\|g.4918 ORF TR6887\|c0_g1_i1\|g.4918 TR6887\|c0_g1_i1\|m.4918 type:complete len:569 (+) TR6887\|c0_g1_i1:244-1950(+) [DBVPG6044_S_cerevisiae-Sep2015-contam.fasta] YKR002W SGDID:S000001710, Chr XI from 443232-444938, Genome Release 64-1-1, Verified ORF, "Poly(A) polymerase, one of three factors required for mRNA 3'-end polyadenylation, forms multiprotein complex with polyadenylation factor I (PF I), also required for mRNA nuclear export; may also polyadenylate rRNAs; required for gene looping" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] asmbl_6674\|g.5044 ORF asmbl_6674\|g.5044 asmbl_6674\|m.5044 type:complete len:569 (+) asmbl_6674:260-1966(+) [Y12_Trinity_v2_2_0_PASA_6May2016-contam.fasta] ID=asmbl_6871\|g.4218 ORF ID=asmbl_6871\|g.4218 ID=asmbl_6871\|m.4218 type:complete len:569 (+) ID=asmbl_6871:243-1949(+) [DBVPG6044_quiverGenomes_Trinity_v2_2_0_PASA_2June2016-contam.fasta] ID=asmbl_6693\|g.4012 ORF ID=asmbl_6693\|g.4012 ID=asmbl_6693\|m.4012 type:complete len:569 (+) ID=asmbl_6693:260-1966(+) [Y12_quiver_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	568 amino acids
Gene Ontology:
Cellular Component:	mRNA cleavage and polyadenylation specificity factor complex [IPI] nucleoplasm [IPI]
Biological Process:	mRNA export from nucleus [IMP] mRNA polyadenylation [IDA][IMP]
Molecular Function:	polynucleotide adenylyltransferase activity [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSSQKVFGIT GPVSTVGATA AENKLNDSLI QELKKEGSFE TEQETANRVQ VLKILQELAQ  60
   61 RFVYEVSKKK NMSDGMARDA GGKIFTYGSY RLGVHGPGSD IDTLVVVPKH VTREDFFTVF 120
  121 DSLLRERKEL DEIAPVPDAF VPIIKIKFSG ISIDLICARL DQPQVPLSLT LSDKNLLRNL 180
  181 DEKDLRALNG TRVTDEILEL VPKPNVFRIA LRAIKLWAQR RAVYANIFGF PGGVAWAMLV 240
  241 ARICQLYPNA CSAVILNRFF IILSEWNWPQ PVILKPIEDG PLQVRVWNPK IYAQDRSHRM 300
  301 PVITPAYPSM CATHNITEST KKVILQEFVR GVQITNDIFS NKKSWANLFE KNDFFFRYKF 360
  361 YLEITAYTRG SDEQHLKWSG LVESKVRLLV MKLEVLAGIK IAHPFTKPFE SSYCCPTEDD 420
  421 YEMIQDKYGS HKTETALNAL KLVTDENKEE ESIKDAPKAY LSTMYIGLDF NIENKKEKVD 480
  481 IHIPCTEFVN LCRSFNEDYG DHKVFNLALR FVKGYDLPDE VFDENEKRPS KKSKRKNLDA 540
  541 RHETVKRSKS DAASGDNING TTAAVDVN

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		CFT1 CFT2 FIP1 GLC7 MPE1 PAP1 PCF11 PFS2 PTA1 PTI1 REF2 RNA14 SSU72 SWD2 YSH1 YTH1
View Details	Krogan NJ, et al. (2006)		AIM1 CFT1 CFT2 ECM21 FIP1 GIP4 MPE1 PAP1 PFS2 PTA1 PTI1 REF2 SSU72 SWD2 SYC1 YBL010C YLR358C YSH1 YTH1
View Details	Gavin AC, et al. (2006)		CFT1 CFT2 FIP1 MPE1 PAP1 PFS2 PTA1 YSH1 YTH1
View Details	Gavin AC, et al. (2002)		ACT1 ADE4 AIM31 BFR2 BMS1 BUD21 CBF5 CFT1 CFT2 CKA1 CLP1 DBP8 DIP2 ECM16 ECM29 EMG1 ENO2 ENP1 ENP2 ERO1 ESF1 ESF2 FIP1 GCN1 GLC7 HAS1 HCA4 HHF1, HHF2 HSL1 HTA1 HTA2 IMP3 IMP4 KRE33 KRR1 MPE1 MPP10 MVD1 NAN1 NOC4 NOP1 NOP12 NOP14 NOP56 NOP58 NOP6 PAP1 PCF11 PFK1 PFS2 PRE6 PRE9 PRP43 PTA1 PTI1 PWP2 REF2 RML2 RNA14 RNA15 ROK1 RPP2B RRP9 RSA3 RTG2 SCL1 SEC13 SEC21 SEC31 SOF1 SRO9 SSA3 SSU72 SYC1 TAF6 TIF1, TIF2 TIF4632 TYE7 UTP10 UTP11 UTP13 UTP15 UTP18 UTP20 UTP21 UTP22 UTP30 UTP4 UTP6 UTP7 UTP8 UTP9 VID24 VMR1 VPS53 YCL046W YSH1 YTH1
View Details	Ho Y, et al. (2002)		DIS3 EAF3 FIP1 HAS1 HPR1 KAP95 MES1 MFT1 NAM8 NOP6 NUP1 NUP2 NUP60 PAP1 PCT1 REB1 RNT1 RRP4 RRP43 RRP6 RTT103 SIF2 SIN3 SNU56 SRP1 STO1 THO2 TRA1 UME1 YPR090W
View Details	Qiu et al. (2008)		CFT1 CFT2 CLP1 HRP1 PAP1 PFS2 REF2 RNA14 RNA15 SWD2 YSH1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	ASF1	3829 - low filter	Green EM, et al (2005)
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	BOI1	Sample bob1 (2nd set) from october 2005	McCusker D, et al (2007)
View Run	MLP2	#24 Asynchronous Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

	PROTEIN(S)	PUBLICATION
View Data	PAP1	Huh WK, et al. (2003)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..38] [191..353]	PSI-BLAST	10166.0	Poly(A) polymerase, middle domain; Poly(A) polymerase N-terminal, catalytic domain; Poly(A) polymerase, C-terminal domain
2	View Details	[39..190] [354..568]	PSI-BLAST	10166.0	Poly(A) polymerase, middle domain; Poly(A) polymerase N-terminal, catalytic domain; Poly(A) polymerase, C-terminal domain

Protein Overview: PAP1

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: