Protein Overview: SKI8

General Information:
Name(s) found:	SKI8 / YGL213C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6321225\|ref\|NP_011302.1\| Protein involved in exosome mediated 3' to 5' mRNA degradation and translation inhibition of non-poly(A) mRNAs as well as double-strand break formation during meiotic recombination; required for repressing propagation of dsRNA [NCBI NR] gi\|49259107\|pdb\|1SQ9\|A Chain A, Structure Of Ski8p, A Wd Repeat Protein Involved In Mrna Degradation And Meiotic Recombination [NCBI NR] gi\|401086\|sp\|Q02793.1\|SKI8_YEAST RecName: Full=Antiviral protein SKI8; AltName: Full=Superkiller protein 8 [NCBI NR] pir\|\|S30023 antiviral protein SKI8 - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|172614\|gb\|AAA35050.1\| antiviral protein [Saccharomyces cerevisiae] [NCBI NR] gi\|1322857\|emb\|CAA96930.1\| SKI8 [Saccharomyces cerevisiae] [NCBI NR] Antiviral protein SKI8 OS=Saccharomyces cerevisiae GN=SKI8 PE=1 SV=1 [Swiss-Prot] SKI8 Antiviral protein of the beta-transducin (WD-40) repeat family [mips] Ski complex component and WD-repeat protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; also required for meiotic double-strand break recombination; null mutants have superkiller phenotype [SGD] SKI8, Chr VII from 90057-91250, reverse complement [yeast_orfs2] Chr VII from 90057-91250, reverse complement [yeastorf3.fasta] Chr VII from 91250-90057, reverse complement [yeastorf5.fasta] (Q02793) Antiviral protein SKI8 [4932.FASTACCERV.txt] SKI8 SGDID:S0003181, Chr VII from 91250-90057, reverse complement, Verified ORF [yeastflysequest.fasta] SKI8 SGDID:S0003181, Chr VII from 91251-90058, reverse complement, Verified ORF [10112005cervSGD.fasta] Ski8p [Saccharomyces cerevisiae]gi\|1322857\|emb\|CAA96930.1\| SKI8 [Saccharomyces cerevisiae]gi\|49259107\|pdb\|1SQ9\|A Chain A, Structure Of Ski8p, A Wd Repeat Protein Involved In Mrna Degradation And Meiotic Recombinationgi\|320634\|pir\|\|S30023 antiviral prot [nrdb_11152004_Chlamydomonas] SKI8 SGDID:S000003181, Chr VII from 91251-90058, reverse complement, Verified ORF, "Protein involved in exosome mediated 3' to 5' mRNA degradation and translation inhibition of non-poly(A) mRNAs as well as double-strand break formation during meiotic reco [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] SKI8 SGDID:S000003181, Chr VII from 91251-90058, reverse complement, Verified ORF, "Ski complex component and WD-repeat protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; also required for meiotic double-strand break recombination; null m [SGD_S-cerevisiae_na_05-08-2009_con_reversed.fasta] Antiviral protein SKI8 [nr-271106-contam.fasta] antiviral protein [nr-271106-contam.fasta] Protein involved in exosome mediated 3' to 5' mRNA degradation and translation inhibition of non-poly(A) mRNAs as well as double-strand break formation during meiotic recombination; required for repressing propagation of dsRNA viruses; Ski8p [Saccharomyce [nr-271106-contam.fasta] Chain A, Structure Of Ski8p, A Wd Repeat Protein Involved In Mrna Degradation And Meiotic Recombination [nr-271106-contam.fasta] SKI8 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] SKI8 SGDID:S000003181, Chr VII from 91251-90058, reverse complement, Verified ORF, "Protein involved in exosome mediated 3' to 5' mRNA degradation and translation inhibition of non-poly(A) mRNAs as well as double-strand break formation during meiotic recombination; required for repressing propagation of dsRNA viruses" [yeast-bovrabprots.fasta] SKI8 SGDID:S000003181, Chr VII from 91251-90058, reverse complement, Verified ORF, "Ski complex component and WD-repeat protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; also required for meiotic double-strand break recombination; null mutants have superkiller phenotype" [scerevisihumanrev.fasta] [chemostatdb.fasta] Antiviral protein SKI8 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=SKI8 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] SKI8 SGDID:S000003181, Chr VII from 91247-90054, Genome Release 64-1-1, reverse complement, Verified ORF, "Ski complex component and WD-repeat protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; also required for meiotic double-strand break recombination; null mutants have superkiller phenotype" [yeast-030211-contam.fasta] SKI8 SGDID:S000003181, Chr VII from 91247-90054, Genome Release 64-2-1, reverse complement, Verified ORF, "Ski complex component and WD-repeat protein; mediates 3'-5' RNA degradation by the cytoplasmic exosome; also required for meiotic double-strand break recombination; null mutants have superkiller phenotype" [20141208_orf_trans.fasta] YGL213C SGDID:S000003181, Chr VII from 91247-90054, Genome Release 64-1-1, reverse complement, Verified ORF, "Ski complex component and WD-repeat protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; also required for meiotic double-strand break recombination; null mutants have superkiller phenotype" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	397 amino acids
Gene Ontology:
Cellular Component:	cytoplasm [IPI] nuclear chromosome [IDA]
Biological Process:	protein complex assembly [IMP] response to exogenous dsRNA [IMP] mRNA catabolic process [IMP][TAS] meiotic DNA double-strand break formation [IPI]
Molecular Function:	translation repressor activity [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSKVFIATAN AGKAHDADIF SVSACNSFTV SCSGDGYLKV WDNKLLDNEN PKDKSYSHFV  60
   61 HKSGLHHVDV LQAIERDAFE LCLVATTSFS GDLLFYRITR EDETKKVIFE KLDLLDSDMK 120
  121 KHSFWALKWG ASNDRLLSHR LVATDVKGTT YIWKFHPFAD ESNSLTLNWS PTLELQGTVE 180
  181 SPMTPSQFAT SVDISERGLI ATGFNNGTVQ ISELSTLRPL YNFESQHSMI NNSNSIRSVK 240
  241 FSPQGSLLAI AHDSNSFGCI TLYETEFGER IGSLSVPTHS SQASLGEFAH SSWVMSLSFN 300
  301 DSGETLCSAG WDGKLRFWDV KTKERITTLN MHCDDIEIEE DILAVDEHGD SLAEPGVFDV 360
  361 KFLKKGWRSG MGADLNESLC CVCLDRSIRW FREAGGK

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		SKI2 SKI3 SKI8 YKL023W
View Details	Krogan NJ, et al. (2006)		ENT5 SKI2 SKI3 SKI8 YKL023W
View Details	Gavin AC, et al. (2006)		SKI2 SKI3 SKI8
View Details	Ho Y, et al. (2002)		AKL1 SKI2 SKI3 SKI8
View Details	Qiu et al. (2008)		SKI2 SKI8

Mass Spectrometry Data

No mass spectrometry results found for this protein.

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	SPO11	SKI8	2	Uetz P, et al. (2000)

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	SKI8	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..397]	PSI-BLAST	61.0	beta1-subunit of the signal-transducing G protein heterotrimer

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
	3.12192428374026	bayes_pls_golite062009
binding	2.42570478946173	bayes_pls_golite062009
RNA binding	2.1927334052131	bayes_pls_golite062009
transcription regulator activity	1.95667720244696	bayes_pls_golite062009
nucleic acid binding	1.90457914882449	bayes_pls_golite062009
general RNA polymerase II transcription factor activity	1.7032681012791	bayes_pls_golite062009
structural molecule activity	1.47448876401574	bayes_pls_golite062009
DNA binding	1.38475369554616	bayes_pls_golite062009
protein binding	1.21879977456466	bayes_pls_golite062009
transcription factor activity	0.804334981068351	bayes_pls_golite062009
cytoskeletal protein binding	0.722742354759599	bayes_pls_golite062009
DNA-dependent ATPase activity	0.625780732991652	bayes_pls_golite062009
transcription repressor activity	0.560226369775279	bayes_pls_golite062009
DNA polymerase activity	0.48827205019881	bayes_pls_golite062009
transcription factor binding	0.412844855996155	bayes_pls_golite062009
snoRNA binding	0.347797037104681	bayes_pls_golite062009
ubiquitin-protein ligase activity	0.2578464839973	bayes_pls_golite062009
transcription cofactor activity	0.19391619547146	bayes_pls_golite062009
DNA helicase activity	0.0403785360444822	bayes_pls_golite062009
transcription corepressor activity	0.00954741955203087	bayes_pls_golite062009
actin binding	0.00606924241186668	bayes_pls_golite062009

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.89

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2