Protein Overview: PTC2

General Information:
Name(s) found:	PTC2 / YER089C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|731491\|sp\|P39966.1\|PP2C2_YEAST RecName: Full=Protein phosphatase 2C homolog 2; Short=PP2C-2 [NCBI NR] gi\|6320934\|ref\|NP_011013.1\| Type 2C protein phosphatase; dephosphorylates Hog1p to limit maximal osmostress induced kinase activity; dephosphorylates Ire1p to downregulate the unfolded protein response; dephosphorylates Cdc28p; role in DNA checkpoint inac [NCBI NR] gi\|603327\|gb\|AAB64644.1\| Ptc2p: Protein phosphotase type II C [Saccharomyces cerevisiae] [NCBI NR] gi\|1628620\|gb\|AAB17392.1\| protein phosphatase type 2C [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S50592 hypothetical protein YER089c - yeast (Saccharomyces cerevisiae) [NCBI NR] Protein phosphatase 2C homolog 2 OS=Saccharomyces cerevisiae GN=PTC2 PE=1 SV=1 [Swiss-Prot] (P39966) Protein phosphatase 2C homolog 2 (EC 3.1.3.16) (PP2C-2) [Swiss-Prot] PTC2 Protein serine/threonine phosphatase of the PP2C family [mips] Type 2C protein phosphatase; dephosphorylates Hog1p to limit maximal osmostress induced kinase activity; dephosphorylates Ire1p to downregulate the unfolded protein response; dephosphorylates Cdc28p; role in DNA checkpoint inactivation [SGD] PTC2, Chr V from 335942-337336, reverse complement [yeast_orfs2] Chr V from 335942-337336, reverse complement [yeastorf3.fasta] Chr V from 337336-335942, reverse complement [yeastorf5.fasta] PTC2 SGDID:S0000891, Chr V from 337336-335942, reverse complement, Verified ORF [yeastflysequest.fasta] Protein phosphatase type 2C [Saccharomyces cerevisiae]gi\|731491\|sp\|P39966\|P2C2_YEAST Protein phosphatase 2C homolog 2 (PP2C-2)gi\|1077683\|pir\|\|S50592 hypothetical protein YER089c - yeast (Saccharomyces cerevisiae)gi\|603327\|gb\|AAB64644.1\| Ptc2p: Protein [nrdb_11152004_Chlamydomonas] PTC2 SGDID:S000000891, Chr V from 337336-335942, reverse complement, Verified ORF, "Type 2C protein phosphatase; dephosphorylates Hog1p to limit maximal osmostress induced kinase activity; dephosphorylates Ire1p to downregulate the unfolded protein respon [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Protein phosphatase 2C homolog 2 (PP2C-2) [nr-271106-contam.fasta] Ptc2p: Protein phosphotase type II C [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Type 2C protein phosphatase; dephosphorylates Hog1p to limit maximal osmostress induced kinase activity; dephosphorylates Ire1p to downregulate the unfolded protein response; dephosphorylates Cdc28p; role in DNA checkpoint inactivation; Ptc2p [Saccharomyc [nr-271106-contam.fasta] protein phosphatase type 2C [Saccharomyces cerevisiae] [nr-271106-contam.fasta] PTC2 SGDID:S000000891, Chr V from 337336-335942, reverse complement, Verified ORF, "Type 2C protein phosphatase; dephosphorylates Hog1p to limit maximal osmostress induced kinase activity; dephosphorylates Ire1p to downregulate the unfolded protein response; dephosphorylates Cdc28p; role in DNA checkpoint inactivation" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Protein phosphatase 2C homolog 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PTC2 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] PTC2 SGDID:S000000891, Chr V from 337340-335946, Genome Release 64-1-1, reverse complement, Verified ORF, "Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p to limit maximal osmostress induced kinase activity; dephosphorylates Ire1p to downregulate the unfolded protein response; dephosphorylates Cdc28p; inactivates the DNA damage checkpoint" [yeast-030211-contam.fasta] PTC2 SGDID:S000000891, Chr V from 337340-335946, Genome Release 64-2-1, reverse complement, Verified ORF, "Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p to limit maximal osmostress induced kinase activity; dephosphorylates Ire1p to downregulate the unfolded protein response; dephosphorylates Cdc28p; inactivates the DNA damage checkpoint; PTC2 has a paralog, PTC3, that arose from the whole genome duplication" [20141208_orf_trans.fasta] TR4710\|c0_g1_i1\|g.3360 ORF TR4710\|c0_g1_i1\|g.3360 TR4710\|c0_g1_i1\|m.3360 type:complete len:465 (+) TR4710\|c0_g1_i1:356-1750(+) [Y12_S_cerevisiae-Sep2015-contam.fasta] TR6449\|c0_g1_i1\|g.4632 ORF TR6449\|c0_g1_i1\|g.4632 TR6449\|c0_g1_i1\|m.4632 type:complete len:465 (+) TR6449\|c0_g1_i1:337-1731(+) [DBVPG6044_S_cerevisiae-Sep2015-contam.fasta] YER089C SGDID:S000000891, Chr V from 337340-335946, Genome Release 64-1-1, reverse complement, Verified ORF, "Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p to limit maximal osmostress induced kinase activity; dephosphorylates Ire1p to downregulate the unfolded protein response; dephosphorylates Cdc28p; inactivates the DNA damage checkpoint" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] asmbl_7290\|g.5333 ORF asmbl_7290\|g.5333 asmbl_7290\|m.5333 type:complete len:465 (+) asmbl_7290:336-1730(+) [Y12_Trinity_v2_2_0_PASA_6May2016-contam.fasta] ID=asmbl_7801\|g.4778 ORF ID=asmbl_7801\|g.4778 ID=asmbl_7801\|m.4778 type:complete len:465 (+) ID=asmbl_7801:336-1730(+) [DBVPG6044_quiverGenomes_Trinity_v2_2_0_PASA_2June2016-contam.fasta] ID=asmbl_7041\|g.4406 ORF ID=asmbl_7041\|g.4406 ID=asmbl_7041\|m.4406 type:complete len:465 (+) ID=asmbl_7041:336-1730(+) [Y12_quiver_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	464 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA] cytoplasm [IDA]
Biological Process:	DNA damage checkpoint [IGI] inactivation of MAPK activity involved in osmosensory signaling pathway [IDA][IGI ][IMP] G1/S transition of mitotic cell cycle [IGI] protein amino acid dephosphorylation [IDA ] response to unfolded protein [IMP][IPI] regulation of cyclin-dependent protein kinase activity [IMP][IPI]
Molecular Function:	protein serine/threonine phosphatase activity [IDA][IEA] phosphoprotein phosphatase activity [IEA] catalytic activity [IEA] metal ion binding [IEA] hydrolase activity [IEA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MGQILSNPVI DKESHSGADS LTAFGLCAMQ GWRMSMEDSH ILEPNVLTKS DKDHIAFYGI  60
   61 FDGHGGAKVA EYCGNKIVEI LQEQKSFHEG NLPRALIDTF INTDVKLLQD PVMKEDHSGC 120
  121 TATSILVSKS QNLLVCGNAG DSRTVLATDG NAKALSYDHK PTLASEKSRI VAADGFVEMD 180
  181 RVNGNLALSR AIGDFEFKSN PKLGPEEQIV TCVPDILEHS LDYDRDEFVI LACDGIWDCL 240
  241 TSQDCVDLVH LGLREGKTLN EISSRIIDVC CAPTTEGTGI GCDNMSIVVV ALLKEGEDVA 300
  301 QWSDRMKSKA HRTSVRSFAD KRRRVFSYYD FSKCNDEQVF AITTKKPQDK FTRDHEAAVA 360
  361 SVTAADNDDP MDIDDTDADT DAENLDPSSQ SKSKTSGPID LASLEALLGA TGGVKTDSNG 420
  421 NKVTYTLPQS ALAQLLQTMG HDPASSHPEN DSNTDHKAGR SHLQ

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		PAA1 PTC2
View Details	Krogan NJ, et al. (2006)		PAA1 PTC2 PTC3 PTC4 VPS34
View Details	Gavin AC, et al. (2006)		ALD6 PTC2 SHQ1
View Details	Ho Y, et al. (2002)		ASF1 CDC13 EDE1 KAP95 PTC2 RAD53 SRP1 SWI4 TBF1 UTP22 YTA7

Mass Spectrometry Data

No mass spectrometry results found for this protein.

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	PTC2	PAA1	2	Uetz P, et al. (2000)
View Screen	YKL036C	PTC2	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	PTC2	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..376]	PSI-BLAST	517.383689	Protein serine/threonine phosphatase 2C, C-terminal domain; Protein serine/threonine phosphatase 2C, catalytic domain
2	View Details	[377..464]	deduced	N/A	No confident structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
phosphoprotein phosphatase activity	7.29765002420868	bayes_pls_golite062009
phosphatase activity	6.84540130415879	bayes_pls_golite062009
phosphoric ester hydrolase activity	6.63839066304808	bayes_pls_golite062009
hydrolase activity, acting on ester bonds	5.76293366458633	bayes_pls_golite062009
protein serine/threonine phosphatase activity	5.66354576593165	bayes_pls_golite062009
hydrolase activity	2.5631136269171	bayes_pls_golite062009
binding	1.74689317666387	bayes_pls_golite062009
catalytic activity	1.53401212718168	bayes_pls_golite062009
protein binding	1.47578617076714	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.98

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2