Protein Overview: PPN1

General Information:
Name(s) found:	PPN1 / YDR452W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|927706\|gb\|AAB64872.1\| Ydr452wp; CAI: 0.15 [Saccharomyces cerevisiae] [NCBI NR] gi\|68625115\|sp\|Q04119.1\|PPN1_YEAST RecName: Full=Endopolyphosphatase; AltName: Full=Exopolyphosphatase; AltName: Full=Phosphate metabolism protein 5; Flags: Precursor [NCBI NR] gi\|6320660\|ref\|NP_010740.1\| Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer; Ppn1p [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S69731 endopolyphosphatase (EC 3.6.1.10) PPN1 precursor [validated] - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|11528443\|gb\|AAG37278.1\| endopolyphosphatase [Saccharomyces cerevisiae] [NCBI NR] Endopolyphosphatase OS=Saccharomyces cerevisiae GN=PPN1 PE=1 SV=1 [Swiss-Prot] PPN1 Vacuolar endopolyphosphatase [mips] Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer [SGD] PHM5, Chr IV from 1362867-1364891 [yeast_orfs2] Chr IV from 1362867-1364891 [yeastorf3.fasta] Chr IV from 1362866-1364890 [yeastorf4.fasta] >Q04119 (Q04119) D9461.37P (ENDOPOLYPHOSPHATASE) [4932.FASTACCERV.txt] PPN1 SGDID:S0002860, Chr IV from 1362866-1364890, Verified ORF [yeastflysequest.fasta] PPN1 SGDID:S0002860, Chr IV from 1362868-1364892, Verified ORF [10112005cervSGD.fasta] Ppn1p [Saccharomyces cerevisiae]gi\|11528443\|gb\|AAG37278.1\| endopolyphosphatase [Saccharomyces cerevisiae]gi\|2131501\|pir\|\|S69731 endopolyphosphatase (EC 3.6.1.10) PPN1 precursor [validated] - yeast (Saccharomyces cerevisiae)gi\|927706\|gb\|AAB64872.1\| Ydr4 [nrdb_11152004_Chlamydomonas] (Q04119) D9461.37P (ENDOPOLYPHOSPHATASE) [cerv4932.FASTAC] PPN1 SGDID:S000002860, Chr IV from 1362868-1364892, Verified ORF, "Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] PPN1 SGDID:S000002860, Chr IV from 1362870-1364894, Verified ORF, "Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer" [yeast-contam.fasta] PPN1 SGDID:S000002860, Chr IV from 1362871-1364895, Verified ORF, "Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer" [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer; Ppn1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Ydr452wp; CAI: 0.15 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] endopolyphosphatase [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Endopolyphosphatase precursor (Exopolyphosphatase) (Phosphate metabolism protein 5) [nr-271106-contam.fasta] [chemostatdb.fasta] Endopolyphosphatase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PPN1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] PPN1 SGDID:S000002860, Chr IV from 1362878-1364902, Genome Release 64-1-1, Verified ORF, "Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer" [yeast-030211-contam.fasta] PPN1 SGDID:S000002860, Chr IV from 1362878-1364902, Genome Release 64-2-1, Verified ORF, "Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer; relocalizes from vacuole to cytoplasm upon DNA replication stress" [20141208_orf_trans.fasta] YDR452W SGDID:S000002860, Chr IV from 1362878-1364902, Genome Release 64-1-1, Verified ORF, "Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	674 amino acids
Gene Ontology:
Cellular Component:	vacuolar membrane [IDA][IMP]
Biological Process:	polyphosphate metabolic process [IDA][IMP]
Molecular Function:	endopolyphosphatase activity [IDA][IMP]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MVVVGKSEVR NVSMSRPKKK SLIAILSTCV LFFLVFIIGA KFQYVSVFSK FLDDRGDNES  60
   61 LQLLNDIEFT RLGLTPREPV IIKDVKTGKE RKLHGRFLHI TDIHPDPYYV EGSSIDAVCH 120
  121 TGKPSKKKDV APKFGKAMSG CDSPVILMEE TLRWIKENLR DKIDFVIWTG DNIRHDNDRK 180
  181 HPRTEAQIFD MNNIVADKMT ELFSAGNEED PRDFDVSVIP SLGNNDVFPH NMFALGPTLQ 240
  241 TREYYRIWKN FVPQQQQRTF DRSASFLTEV IPGKLAVLSI NTLYLFKANP LVDNCNSKKE 300
  301 PGYQLLLWFG YVLEELRSRG MKVWLSGHVP PIAKNFDQSC YDKFTLWTHE YRDIIIGGLY 360
  361 GHMNIDHFIP TDGKKARKSL LKAMEQSTRV QQGEDSNEED EETELNRILD HAMAAKEVFL 420
  421 MGAKPSNKEA YMNTVRDTYY RKVWNKLERV DEKNVENEKK KKEKKDKKKK KPITRKELIE 480
  481 RYSIVNIGGS VIPTFNPSFR IWEYNITDIV NDSNFAVSEY KPWDEFFESL NKIMEDSLLE 540
  541 DEMDSSNIEV GINREKMGEK KNKKKKKNDK TMPIEMPDKY ELGPAYVPQL FTPTRFVQFY 600
  601 ADLEKINQEL HNSFVESKDI FRYEIEYTSD EKPYSMDSLT VGSYLDLAGR LYENKPAWEK 660
  661 YVEWSFASSG YKDD

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Krogan NJ, et al. (2006)		CWP2 DBP2 FLC2 PPN1 RPS31 SRP40 TFA1 TFA2 YAP1801

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	VAM3	sample: hx3	Hao Xu, et al. (2010)
View Run	VAM3	sample: hx4	Hao Xu, et al. (2010)
View Run	VAM3	Sample: HX11 - both fusion and trans-SNARE complex formation take place.	Hao Xu, et al. (2010)
View Run	VAM3	Sample: HX12 - trans-SNARE complex formation and fusion are inhibited (control).	Hao Xu, et al. (2010)
View Run	VAM3	Sample: HX14 - mixture in detergent (control).	Hao Xu, et al. (2010)
View Run	VAM3	hx23: mixture in detergent (control) Investigating Vam3p, Nyv1p and their partners in trans-SNARE complex	Hao Xu, et al. (2010)
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

	PROTEIN(S)	PUBLICATION
View Data	PPN1	Huh WK, et al. (2003)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..394]	ORFEUS	13.8	Mammalian purple acid phosphatase
2	View Details	[395..674]	deduced	N/A	No confident structure predictions are available.

Protein Overview: PPN1

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: