Protein Overview: MFB1

General Information:
Name(s) found:	MFB1 / YDR219C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|74644873\|sp\|Q04922.1\|MFB1_YEAST RecName: Full=Mitochondrial F-box protein MFB1 [NCBI NR] gi\|728675\|emb\|CAA88499.1\| unknown [Saccharomyces cerevisiae] [NCBI NR] gi\|6320425\|ref\|NP_010505.1\| Mitochondria-associated F-box protein involved in maintenance of normal mitochondrial morphology; interacts with Skp1p through the F-box motif; preferentially localizes to the mother cell during budding; Mfb1p [Saccharomyces ce [NCBI NR] pir\|\|S59426 hypothetical protein YDR219c - yeast (Saccharomyces cerevisiae) [NCBI NR] Mitochondrial F-box protein MFB1 OS=Saccharomyces cerevisiae GN=MFB1 PE=1 SV=1 [Swiss-Prot] MFB1 Mitochondrial protein, putative involved in ubiquitin-dependent protein catabolism [mips] Mitochondria-associated F-box protein involved in maintenance of normal mitochondrial morphology; interacts with Skp1p through the F-box motif; preferentially localizes to the mother cell during budding [SGD] YDR219C, Chr IV from 905446-906843, reverse complement [yeast_orfs2] Chr IV from 905446-906843, reverse complement [yeastorf3.fasta] Chr IV from 906843-905446, reverse complement [yeastorf5.fasta] >Q04922 (Q04922) Hypothetical protein [4932.FASTACCERV.txt] YDR219C SGDID:S0002627, Chr IV from 906844-905447, reverse complement, Uncharacterized ORF [yeastflysequest.fasta] YDR219C SGDID:S0002627, Chr IV from 906846-905449, reverse complement, Uncharacterized ORF [10112005cervSGD.fasta] Ydr219cp [Saccharomyces cerevisiae]gi\|728675\|emb\|CAA88499.1\| unknown [Saccharomyces cerevisiae]gi\|1363778\|pir\|\|S59426 hypothetical protein YDR219c - yeast (Saccharomyces cerevisiae) [nrdb_11152004_Chlamydomonas] (Q04922) Hypothetical protein [cerv4932.FASTAC] YDR219C SGDID:S000002627, Chr IV from 906846-905449, reverse complement, Uncharacterized ORF, "Mitochondria-associated F-box protein involved in maintenance of normal mitochondrial morphology" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] MFB1 SGDID:S000002627, Chr IV from 906848-905451, reverse complement, Verified ORF, "Mitochondria-associated F-box protein involved in maintenance of normal mitochondrial morphology; interacts with Skp1p through the F-box motif; preferentially localizes t [yeast-contam.fasta] MFB1 SGDID:S000002627, Chr IV from 906849-905452, reverse complement, Verified ORF, "Mitochondria-associated F-box protein involved in maintenance of normal mitochondrial morphology; interacts with Skp1p through the F-box motif; preferentially localizes t [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] Mitochondria-associated F-box protein involved in maintenance of normal mitochondrial morphology; interacts with Skp1p through the F-box motif; preferentially localizes to the mother cell during budding; Mfb1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] unknown [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Mitochondrial F-box protein MFB1 [nr-271106-contam.fasta] MFB1 SGDID:S000002627, Chr IV from 906849-905452, reverse complement, Verified ORF, "Mitochondria-associated F-box protein involved in maintenance of normal mitochondrial morphology; interacts with Skp1p through the F-box motif; preferentially localizes to the mother cell during budding" [yeast-bovrabprots.fasta] MFB1 SGDID:S000002627, Chr IV from 906848-905451, reverse complement, Verified ORF, "Mitochondria-associated F-box protein involved in maintenance of normal mitochondrial morphology; interacts with Skp1p through the F-box motif; preferentially localizes to the mother cell during budding" [yeast-contam_custom.fasta] [chemostatdb.fasta] Mitochondrial F-box protein MFB1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=MFB1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] MFB1 SGDID:S000002627, Chr IV from 906852-905455, Genome Release 64-1-1, reverse complement, Verified ORF, "Mitochondria-associated F-box protein involved in maintenance of normal mitochondrial morphology; interacts with Skp1p through the F-box motif; preferentially localizes to the mother cell during budding" [yeast-030211-contam.fasta] MFB1 SGDID:S000002627, Chr IV from 906852-905455, Genome Release 64-2-1, reverse complement, Verified ORF, "Mitochondria-associated F-box protein; involved in maintenance of normal mitochondrial morphology; interacts with Skp1p through the F-box motif; preferentially localizes to the mother cell during budding" [20141208_orf_trans.fasta] YDR219C SGDID:S000002627, Chr IV from 906852-905455, Genome Release 64-1-1, reverse complement, Verified ORF, "Mitochondria-associated F-box protein involved in maintenance of normal mitochondrial morphology; interacts with Skp1p through the F-box motif; preferentially localizes to the mother cell during budding" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	465 amino acids
Gene Ontology:
Cellular Component:	ubiquitin ligase complex [ISS] extrinsic to mitochondrial outer membrane [IDA]
Biological Process:	mitochondrion organization [IMP] ubiquitin-dependent protein catabolic process [ISS]
Molecular Function:	protein binding [ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MTLFSCSVQM PLEERSLTNL PLNLLFRILS HLDMNDLQNI GKTCTLLRML ANENIVYRNA  60
   61 VIGSNGNMWW TKNVLVDVFD VLNFNRKAMK TLNSHNISLV ASLRNVQRKY KLGVIDPARK 120
  121 TISYRTNEVE SKEKGSVKDL NMDLNEPTEI TREQIAHTAI LQGMNQFIEL NDKAFRTHSA 180
  181 DSDDTYIEEN NGEIHSLHGL EKNTTFEEDL VKKPPFIPSP TFSNYSRSST NSVFSSSSPK 240
  241 LLDDDWNNIT MDFTKSRDPD YKEMTPTSTE SSDSITRLRK SNKVKDKAEL FEKLIFRDSR 300
  301 PLKTKKKDNP RLKLSSSLSA NDEDFRKIIS PPSDILPKVG RRSVSRGYLE EIERHYPDFN 360
  361 GETTNPLAIK RVNSTKIANY EQLIIKENSS NCKGITEKND ENKFQRSHTS PVIELSKPHQ 420
  421 RSKLKAVVTD GNKICYRKIE LDNPSGSNTN DHVIKRLDAN TDFNI

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Ho Y, et al. (2002)		MFB1 SKP1 YHR122W

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	MLP2	#25 Asynchronous Prep3-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#21 Asynchronous Prep2-IMAC Phosphopeptide enrichment A1	Keck JM, et al. (2011)
View Run	MLP2	#14 Mitotic Prep2-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	MFB1	DSE1	1	Unpublished Fields Lab Data
View Screen	MFB1	GDS1	1	Unpublished Fields Lab Data
View Screen	MFB1	YGL024W	1	Unpublished Fields Lab Data
View Screen	MFB1	ATF1	1	Unpublished Fields Lab Data
View Screen	MFB1	PHB2	1	Unpublished Fields Lab Data
View Screen	MFB1	BMS1	1	Unpublished Fields Lab Data
View Screen	MFB1	QNS1	1	Unpublished Fields Lab Data
View Screen	MFB1	SUE1	1	Unpublished Fields Lab Data
View Screen	MFB1	PPX1	1	Unpublished Fields Lab Data
View Screen	MFB1	MLC2	1	Unpublished Fields Lab Data
View Screen	MFB1	IMD2	1	Unpublished Fields Lab Data
View Screen	SKP1	MFB1	1	Unpublished Fields Lab Data
View Screen	MFB1	EMC2	1	Unpublished Fields Lab Data
View Screen	UBC7	MFB1	1	Unpublished Fields Lab Data
View Screen	MFB1	CAP1	1	Unpublished Fields Lab Data
View Screen	SGT1	MFB1	1	Unpublished Fields Lab Data
View Screen	MFB1	TTI1	1	Unpublished Fields Lab Data
View Screen	MFB1	SRY1	1	Unpublished Fields Lab Data
View Screen	MFB1	YBL081W	1	Unpublished Fields Lab Data
View Screen	MFB1	HOF1	1	Unpublished Fields Lab Data
View Screen	MFB1	YBR056W	1	Unpublished Fields Lab Data
View Screen	MFB1	MIH1	1	Unpublished Fields Lab Data
View Screen	MFB1	ASF2	1	Unpublished Fields Lab Data
View Screen	MFB1	YMR102C	1	Unpublished Fields Lab Data
View Screen	MFB1	RAD55	1	Unpublished Fields Lab Data
View Screen	MFB1	BIO5	1	Unpublished Fields Lab Data
View Screen	MFB1	TVP15	1	Unpublished Fields Lab Data
View Screen	MFB1	YOL160W	1	Unpublished Fields Lab Data
View Screen	MFB1	RTT105	1	Unpublished Fields Lab Data
View Screen	MFB1	SWT1	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	MFB1	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..126]	Pfam	4.29243	F-box domain No confident structure predictions are available.
2	View Details	[127..465]	deduced	N/A	No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2