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Protein Overview: AMN1

General Information:
Name(s) found: AMN1 / YBR158W [SGD]
Description(s) found:
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  • gi|6319634|ref|NP_009716.1| Protein required for daughter cell separation, multiple mitotic checkpoints, and chromosome stability; contains 12 degenerate leucine-rich repeat motifs; expression is induced by the Mitotic Exit Network (MEN); Amn1p [Saccharom [NCBI NR]
  • pir||S46029 hypothetical protein YBR158w - yeast (Saccharomyces cerevisiae) [NCBI NR]
  • gi|586298|sp|P38285.1|AMN1_YEAST RecName: Full=Antagonist of mitotic exit network protein 1; AltName: Full=Chromosome stability protein 13; AltName: Full=Increased copper-sensitivity protein 4 [NCBI NR]
  • gi|536490|emb|CAA85117.1| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR]
  • gi|51830206|gb|AAU09677.1| YBR158W [Saccharomyces cerevisiae] [NCBI NR]
  • (P38285) Uncharacterized protein YBR158W [Swiss-Prot]
  • Antagonist of mitotic exit network protein 1 OS=Saccharomyces cerevisiae GN=AMN1 PE=1 SV=1 [Swiss-Prot]
  • AMN1 Antagonist of Mitotic exit Network [mips]
  • Protein required for daughter cell separation, multiple mitotic checkpoints, and chromosome stability; contains 12 degenerate leucine-rich repeat motifs; expression is induced by the Mitotic Exit Network (MEN) [SGD]
  • CST13, Chr II from 556506-558155 [yeast_orfs2]
  • Chr II from 556506-558155 [yeastorf3.fasta]
  • Chr II from 556505-558154 [yeastorf4.fasta]
  • Chr II from 556503-558152 [yeastorf5.fasta]
  • (P38285) Hypothetical 62.7 kDa protein in CNS1-CDC28 intergenic region [4932.FASTACCERV.txt]
  • AMN1 SGDID:S0000362, Chr II from 556505-558154, Verified ORF [yeastflysequest.fasta]
  • AMN1 SGDID:S0000362, Chr II from 556543-558192, Verified ORF [10112005cervSGD.fasta]
  • Amn1p [Saccharomyces cerevisiae]gi|51830206|gb|AAU09677.1| YBR158W [Saccharomyces cerevisiae]gi|536490|emb|CAA85117.1| unnamed protein product [Saccharomyces cerevisiae]gi|626530|pir||S46029 hypothetical protein YBR158w - yeast (Saccharomyces cerevisia [nrdb_11152004_Chlamydomonas]
  • AMN1 SGDID:S000000362, Chr II from 556543-558192, Verified ORF, "Protein required for daughter cell separation, multiple mitotic checkpoints, and chromosome stability; contains 12 degenerate leucine-rich repeat motifs; expression is induced by the Mitotic [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta]
  • YBR158W [Saccharomyces cerevisiae] [nr-271106-contam.fasta]
  • Protein required for daughter cell separation, multiple mitotic checkpoints, and chromosome stability; contains 12 degenerate leucine-rich repeat motifs; expression is induced by the Mitotic Exit Network (MEN); Amn1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta]
  • unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta]
  • Uncharacterized protein YBR158W [nr-271106-contam.fasta]
  • AMN1 SGDID:S000000362, Chr II from 556543-558192, Verified ORF, "Protein required for daughter cell separation, multiple mitotic checkpoints, and chromosome stability; contains 12 degenerate leucine-rich repeat motifs; expression is induced by the Mitotic Exit Network (MEN)" [yeast-bovrabprots.fasta]
  • [chemostatdb.fasta]
  • Antagonist of mitotic exit network protein 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=AMN1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta]
  • AMN1 SGDID:S000000362, Chr II from 556549-558198, Genome Release 64-1-1, Verified ORF, "Protein required for daughter cell separation, multiple mitotic checkpoints, and chromosome stability; contains 12 degenerate leucine-rich repeat motifs; expression is induced by the Mitotic Exit Network (MEN)" [yeast-030211-contam.fasta]
  • AMN1 SGDID:S000000362, Chr II from 556549-558198, Genome Release 64-2-1, Verified ORF, "Protein required for daughter cell separation; multiple mitotic checkpoints, and chromosome stability; contains 12 degenerate leucine-rich repeat motifs; expression is induced by the Mitotic Exit Network (MEN)" [20141208_orf_trans.fasta]
  • YBR158W SGDID:S000000362, Chr II from 556549-558198, Genome Release 64-1-1, Verified ORF, "Protein required for daughter cell separation, multiple mitotic checkpoints, and chromosome stability; contains 12 degenerate leucine-rich repeat motifs; expression is induced by the Mitotic Exit Network (MEN)" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
    • Organism: Saccharomyces cerevisiae
    Length: 549 amino acids

    Gene Ontology:
    Cellular Component: nucleus [IDALink to PubMed article]
    cellular bud [IDALink to PubMed article]
    cytoplasm [IDALink to PubMed article]
    Biological Process: negative regulation of exit from mitosis [IGILink to PubMed article][IMPLink to PubMed article]
    mitotic cell cycle checkpoint [IMPLink to PubMed article]
    Molecular Function: protein binding [IPILink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MKLERVSSNG SFKRGRDIQS LESPCTRPLK KMSPSPSFTS LKMEKPFKDI VRKYGGHLHQ  60
   61 SSYNPGSSKV ELVRPDLSLK TDQSFLQSSV QTTPNKKSCN EYLSTPEATP LKNTATENAW 120
  121 ATSRVVSASS LSIVTPTEIK NILVDEFSEL KLGQPLTAQH QRSHAVFEIP EIVENIIKMI 180
  181 VSLESANIPK ERPCLRRNPQ SYEHSLLMYK DEERAKKAWS AAQQLRDPPL VGHKEKKQGA 240
  241 LFSCMMVNRL WLNVTRPFLF KSLHFKSVHN FKEFLRTSQE TTQVMRPSHF ILHKLHQVTQ 300
  301 PDIERLSRME CQNLKWLEFY VCPRITPPLS WFDNLHKLEK LIIPGNKNID DNFLLRLSQS 360
  361 IPNLKHLVLR ACDNVSDSGV VCIALNCPKL KTFNIGRHRR GNLITSVSLV ALGKYTQVET 420
  421 VGFAGCDVDD AGIWEFARLN GKNVERLSLN SCRLLTDYSL PILFALNSFP NLAVLEIRNL 480
  481 DKITDVRHFV KYNLWKKSLD APILIEACER ITKLIDQEEN RVKRINSLVA LKDMTAWVNA 540
  541 DDEIENNVD

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Hazbun TR, et al. (2003) AMN1 AXL2 IMH1 PEX19 PFK27 RAX2 RGT2 RIC1 RIX7 RPA190 SST2 TRX1 TRX2 YLR035C-A
    View Details Gavin AC, et al. (2002) AMN1 ASC1 BEM2 BUD14 ESP1 GTR2 KIN3 MIS1 RVB2 SCP160 SKI3 SPT16 SSZ1 TIF4631 YCH1 YCR015C ZUO1

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run RIX7 No Comments Hazbun TR, et al. (2003)
    View Run MLP2 #34 Asynchronous SPB prep Keck JM, et al. (2011)
    View Run MLP2 #12 Mitotic Prep1-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #11 Mitotic Prep1-TiO2 enriched, new search criteria Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

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      PROTEIN(S) PUBLICATION
    View Data AMN1 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..65] deduced N/A No confident structure predictions are available.
    2 View Details [66..156] PSI-BLAST 7.39794 Leucine rich effector protein YopM
    3 View Details [157..233] PSI-BLAST 7.522879 Ribonuclease inhibitor
    4 View Details [234..296] PSI-BLAST 7.522879 Ribonuclease inhibitor
    5 View Details [297..347] PSI-BLAST 7.522879 Ribonuclease inhibitor
    6 View Details [348..549] PSI-BLAST 7.522879 Ribonuclease inhibitor


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.98

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle