Protein Overview: PTC4

General Information:
Name(s) found:	PTC4 / YBR125C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6319601\|ref\|NP_009683.1\| Cytoplasmic type 2C protein phosphatase; identified as a high-copy number suppressor of the synthetic lethality of a cnb1 mpk1 double deletion; overexpression decreases high-osmolarity induced Hog1p phosphorylation and kinase a [NCBI NR] gi\|586537\|sp\|P38089.1\|PP2C4_YEAST RecName: Full=Protein phosphatase 2C homolog 4; Short=PP2C-4 [NCBI NR] gi\|536409\|emb\|CAA85082.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] gi\|476078\|emb\|CAA55626.1\| hyp. protein YBL0513 [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S48288 probable phosphoprotein phosphatase (EC 3.1.3.16) YBR0921 - yeast (Saccharomyces cerevisiae) [NCBI NR] Protein phosphatase 2C homolog 4 OS=Saccharomyces cerevisiae GN=PTC4 PE=1 SV=1 [Swiss-Prot] (P38089) Protein phosphatase 2C homolog 4 (EC 3.1.3.16) (PP2C-4) [Swiss-Prot] PTC4 Serine/threonine protein phosphatases (PP2Cs) [mips] Cytoplasmic type 2C protein phosphatase; identified as a high-copy number suppressor of the synthetic lethality of a cnb1 mpk1 double deletion; overexpression decreases high-osmolarity induced Hog1p phosphorylation and kinase activity [SGD] PTC4, Chr II from 487157-488338, reverse complement [yeast_orfs2] Chr II from 487157-488338, reverse complement [yeastorf3.fasta] Chr II from 487156-488337, reverse complement [yeastorf4.fasta] Chr II from 488335-487154, reverse complement [yeastorf5.fasta] PTC4 SGDID:S0000329, Chr II from 488336-487155, reverse complement, Verified ORF [yeastflysequest.fasta] PTC4 SGDID:S0000329, Chr II from 488374-487193, reverse complement, Verified ORF [10112005cervSGD.fasta] Phosphatase type Two C; Type 2C protein phosphatase [Saccharomyces cerevisiae]gi\|476078\|emb\|CAA55626.1\| hyp. protein YBL0513 [Saccharomyces cerevisiae]gi\|536409\|emb\|CAA85082.1\| unnamed protein product [Saccharomyces cerevisiae]gi\|586537\|sp\|P38089\|P2C4_ [nrdb_11152004_Chlamydomonas] PTC4 SGDID:S000000329, Chr II from 488374-487193, reverse complement, Verified ORF, "Cytoplasmic type 2C protein phosphatase; identified as a high-copy number suppressor of the synthetic lethality of a cnb1 mpk1 double deletion; overexpression decreases h [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Cytoplasmic type 2C protein phosphatase; identified as a high-copy number suppressor of the synthetic lethality of a cnb1 mpk1 double deletion; overexpression decreases high-osmolarity induced Hog1p phosphorylation and kinase activity; Ptc4p [Saccharomyce [nr-271106-contam.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] hyp. protein YBL0513 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Protein phosphatase 2C homolog 4 (PP2C-4) [nr-271106-contam.fasta] PTC4 SGDID:S000000329, Chr II from 488374-487193, reverse complement, Verified ORF, "Cytoplasmic type 2C protein phosphatase; identified as a high-copy number suppressor of the synthetic lethality of a cnb1 mpk1 double deletion; overexpression decreases high-osmolarity induced Hog1p phosphorylation and kinase activity" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Protein phosphatase 2C homolog 4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PTC4 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] PTC4 SGDID:S000000329, Chr II from 488380-487199, Genome Release 64-1-1, reverse complement, Verified ORF, "Cytoplasmic type 2C protein phosphatase (PP2C); identified as a high-copy number suppressor of cnb1 mpk1 synthetic lethality; overexpression decreases high-osmolarity induced Hog1p phosphorylation and kinase activity" [yeast-030211-contam.fasta] PTC4 SGDID:S000000329, Chr II from 488380-487199, Genome Release 64-2-1, reverse complement, Verified ORF, "Cytoplasmic type 2C protein phosphatase (PP2C); identified as a high-copy number suppressor of cnb1 mpk1 synthetic lethality; overexpression decreases high-osmolarity induced Hog1p phosphorylation and kinase activity" [20141208_orf_trans.fasta] TR4381\|c0_g1_i1\|g.2922 ORF TR4381\|c0_g1_i1\|g.2922 TR4381\|c0_g1_i1\|m.2922 type:complete len:394 (+) TR4381\|c0_g1_i1:196-1377(+) [DBVPG6044_S_cerevisiae-Sep2015-contam.fasta] YBR125C SGDID:S000000329, Chr II from 488380-487199, Genome Release 64-1-1, reverse complement, Verified ORF, "Cytoplasmic type 2C protein phosphatase (PP2C); identified as a high-copy number suppressor of cnb1 mpk1 synthetic lethality; overexpression decreases high-osmolarity induced Hog1p phosphorylation and kinase activity" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] ID=asmbl_4731\|g.3047 ORF ID=asmbl_4731\|g.3047 ID=asmbl_4731\|m.3047 type:complete len:394 (+) ID=asmbl_4731:197-1378(+) [DBVPG6044_quiverGenomes_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	393 amino acids
Gene Ontology:
Cellular Component:	cytoplasm [IDA]
Biological Process:	biological_process [ND]
Molecular Function:	protein serine/threonine phosphatase activity [IDA][IEA][ISS] phosphoprotein phosphatase activity [IEA] metal ion binding [IEA] catalytic activity [IEA] hydrolase activity [IEA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MGQLLSHPLT EKTIEYNEYK NNQASTGIVP RFYNCVGSMQ GYRLTQEDAH LIRNENSVVY  60
   61 VRFFNPFIDK YETLSLNVFA VFDGHGGDDC SKFLSGGRHH RDGNGSSNGN GEPNAGLIKW 120
  121 IAYSFENHHY TSTTNNDSSK FKRSFNTLEG LVSQIFKDAF ILQDEELYRH FANSSCGSTA 180
  181 VVACIINEES LYVANCGDSR CILSSKSNGI KTMSFDHKPQ HIGELIRIND NGGTVSLGRV 240
  241 GGVLALSRAF SDFQFKRGVT YPHRRTKLTN ITQNLTYGTP PQEAQVTVEP DVLMHKIDYS 300
  301 KDEFLVLACD GIWDIYNNKQ LIHFIKYHLV SGTKLDTIIT KLLDHGIAQA NSNTGVGFDN 360
  361 MTAIIVVLNR KGETLQDWFN KMKTRLERER GLV

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		HAT1 HAT2 HIF1 PAA1 PTC4
View Details	Krogan NJ, et al. (2006)		PAA1 PTC2 PTC3 PTC4 VPS34
View Details	Gavin AC, et al. (2006)		PTC4 YDR186C
View Details	Gavin AC, et al. (2002)		PAA1 PTC4 YDR186C
View Details	Ho Y, et al. (2002)		ABP1 ACO1 AIM41 ATP4 BUD14 CAR2 COR1 CRZ1 CYS4 DCP1 DCP2 DNM1 EDC3 EDE1 EGD2 ENP1 GAS1 GCN3 GIN4 GPH1 HHF1, HHF2 HRR25 HSP104 HXT7 HYP2 IPP1 LOC1 LSP1 LTV1 MDH1 MDS3 MER1 MGE1 NSA1 NUG1 NUM1 OYE2 PAA1 PEX19 PIL1 PIN4 PTC4 PUF3 RIO2 RPC19 RPM2 SAP185 SAP190 SAS10 SEC2 SEC23 SEF1 SES1 SFB3 SGM1 SIT4 SKT5 SPT16 SYF1 TMA19 TPS1 TSR1 VHS1 VMA4 YBR225W YER138C

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	None Entered	#17 Mitotic Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	PTC4	YIL082W	2	Unpublished Fields Lab Data
View Screen	PTC4	PAA1	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	PTC4	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..393]	PSI-BLAST	336.70927	Protein serine/threonine phosphatase 2C, C-terminal domain; Protein serine/threonine phosphatase 2C, catalytic domain

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
phosphoprotein phosphatase activity	7.29765002420868	bayes_pls_golite062009
phosphatase activity	6.84540130415879	bayes_pls_golite062009
phosphoric ester hydrolase activity	6.63839066304808	bayes_pls_golite062009
protein serine/threonine phosphatase activity	6.20846750257603	bayes_pls_golite062009
hydrolase activity, acting on ester bonds	5.76293366458633	bayes_pls_golite062009
hydrolase activity	2.93014592649821	bayes_pls_golite062009
binding	1.74689317666387	bayes_pls_golite062009
signal transducer activity	1.61688496161233	bayes_pls_golite062009
molecular transducer activity	1.61688496161233	bayes_pls_golite062009
catalytic activity	1.53401212718168	bayes_pls_golite062009
protein binding	1.47578617076714	bayes_pls_golite062009

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2