Protein Overview: ECM33

General Information:
Name(s) found:	ECM33 / YBR078W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6319552\|ref\|NP_009634.1\| GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p; Ecm33p [Saccharomyces cer [NCBI NR] pir\|\|S70297 SPS2 protein homolog YBR078w - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1872127\|emb\|CAA85022.1\| ECM33 [Saccharomyces cerevisiae] [NCBI NR] ECM33 Protein involved in cell wall biogenesis and architecture [mips] GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p [SGD] ECM33, Chr II from 393082-393139,393470-394818 [yeast_orfs2] Chr II from 393082-393139,393470-394818 [yeastorf3.fasta] Chr II from 393080-393137,393468-394816 [yeastorf5.fasta] ECM33 SGDID:S0000282, Chr II from 393081-393138,393469-394817, Verified ORF [yeastflysequest.fasta] ECM33 SGDID:S0000282, Chr II from 393118-393175,393506-394854, Verified ORF [10112005cervSGD.fasta] Ecm33p [Saccharomyces cerevisiae]gi\|1872127\|emb\|CAA85022.1\| ECM33 [Saccharomyces cerevisiae]gi\|2133150\|pir\|\|S70297 SPS2 protein homolog YBR078w - yeast (Saccharomyces cerevisiae) [nrdb_11152004_Chlamydomonas] ECM33 SGDID:S000000282, Chr II from 393118-393175,393506-394854, Verified ORF, "GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; similar to Sps2p and Pst1p" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] ECM33 SGDID:S000000282, Chr II from 393118-393175,393506-394854, Verified ORF, "GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; s [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; similar to Sps2p and Pst1p; Ecm33p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] ECM33 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] ECM33 SGDID:S000000282, Chr II from 393118-393175,393506-394854, Verified ORF, "GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p" [yeast-bovrabprots.fasta] [chemostatdb.fasta]
Organism:	Saccharomyces cerevisiae
Length:	468 amino acids
Gene Ontology:
Cellular Component:	fungal-type cell wall [IDA] mitochondrion [IDA ] plasma membrane [IDA]
Biological Process:	cellular cell wall organization [IMP]
Molecular Function:	molecular_function [ND]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MQFKNALTAT AILSASALAA NSTTSIPSSC SIGTSATATA QADLDKISGC STIVGNLTIT  60
   61 GDLGSAALAS IQEIDGSLTI FNSSSLSSFS ADSIKKITGD LNMQELIILT SASFGSLQEV 120
  121 DSINMVTLPA ISTFSTDLQN ANNIIVSDTT LESVEGFSTL KKVNVFNINN NRYLNSFQSS 180
  181 LESVSDSLQF SSNGDNTTLA FDNLVWANNI TLRDVNSISF GSLQTVNASL GFINNTLPSL 240
  241 NLTQLSKVGQ SLSIVSNDEL SKAAFSNLTT VGGGFIIANN TQLKVIDGFN KVQTVGGAIE 300
  301 VTGNFSTLDL SSLKSVRGGA NFDSSSSNFS CNALKKLQSN GAIQGDSFVC KNGATSTSVK 360
  361 LSSTSTESSK SSATSSASSS GDASNAQANV SASASSSSSS SKKSKGAAPE LVPATSFMGV 420
  421 VAAVGVAYYK IKATICVSII TLISSLMISL PFLFYYETVG SSLNFICR

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Ho Y, et al. (2002)		ADR1 BBC1 CDC39 CDC53 CRM1 DUR1,2 ECM29 ECM33 FAA4 GAL3 GCN1 HRT1 HYP2 MDN1 MKT1 MMS1 MYO2 PFK1 PMA2 RPA190 RPN1 TPS1 UBI4 VPS13 YAR009C YGP1 YLR035C-A

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#24 Asynchronous Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#31 Asynchronous Prep (Protease cleavage)	Keck JM, et al. (2011)
View Run	MLP2	#03 Alpha Factor Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	NAF1	ECM33	2	Hazbun TR, et al. (2003)

Microscopy / Localization Data

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No microscopy data found in the PDR for this protein.

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..91]	PSI-BLAST	21.522879	No description for 1dnqA_ was found.
2	View Details	[92..468]	PSI-BLAST	8.69897	Leucine rich effector protein YopM

Functions predicted (by domain):

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
receptor activity	1.46706519505919	bayes_pls_golite062009
binding	1.29581524164992	bayes_pls_golite062009
transmembrane receptor activity	1.1862168530335	bayes_pls_golite062009
protein binding	0.853548241721519	bayes_pls_golite062009
signal transducer activity	0.499118819144462	bayes_pls_golite062009
molecular transducer activity	0.499118819144462	bayes_pls_golite062009

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

[View Details]

Source: Reynolds et al. 2008. Manuscript submitted

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2