Protein Overview: MTW1

General Information:
Name(s) found:	MTW1 / YAL034W-A [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6319283\|ref\|NP_009367.1\| Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly; Mtw1p [Saccharomyces ce [NCBI NR] gi\|4887606\|dbj\|BAA77792.1\| Mtw1p [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S70293 hypothetical protein YAL034w-a - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1723189\|sp\|P39731.2\|MTW1_YEAST RecName: Full=Kinetochore-associated protein MTW1; AltName: Full=Mis12-like protein [NCBI NR] gi\|1326057\|gb\|AAC04997.1\| Yal034w-ap [Saccharomyces cerevisiae] [NCBI NR] Kinetochore-associated protein MTW1 OS=Saccharomyces cerevisiae GN=MTW1 PE=1 SV=2 [Swiss-Prot] MTW1 Determining metaphase spindle length [mips] Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly [SGD] MTW1, Chr I from 79720-80589 [yeast_orfs2] Chr I from 79720-80589 [yeastorf3.fasta] (P39731) Protein MTW1 (Mis12-like protein) [4932.FASTACCERV.txt] MTW1 SGDID:S0000032, Chr I from 79721-80590, Verified ORF [yeastflysequest.fasta] MTW1 SGDID:S0000032, Chr I from 79719-80588, Verified ORF [10112005cervSGD.fasta] Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly [Saccharomyces cerevisiae]gi\|2131304\|pir\|\|S70293 hy [nrdb_11152004_Chlamydomonas] MTW1 SGDID:S000000032, Chr I from 79719-80588, Verified ORF, "Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Kinetochore-associated protein MTW1 (Mis12-like protein) [nr-271106-contam.fasta] Mtw1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Yal034w-ap [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly; Mtw1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] MTW1 SGDID:S000000032, Chr I from 79719-80588, Verified ORF, "Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Kinetochore-associated protein MTW1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=MTW1 PE=1 SV=2 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] MTW1 SGDID:S000000032, Chr I from 79718-80587, Genome Release 64-1-1, Verified ORF, "Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly" [yeast-030211-contam.fasta] MTW1 SGDID:S000000032, Chr I from 79718-80587, Genome Release 64-2-1, Verified ORF, "Essential component of the MIND kinetochore complex; joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly; complex consists of Mtw1p Including Nnf1p-Nsl1p-Dsn1p (MIND)" [20141208_orf_trans.fasta] TR2495\|c0_g1_i1\|g.1872 ORF TR2495\|c0_g1_i1\|g.1872 TR2495\|c0_g1_i1\|m.1872 type:complete len:290 (+) TR2495\|c0_g1_i1:46-915(+) [Y12_S_cerevisiae-Sep2015-contam.fasta] TR2591\|c0_g1_i1\|g.1794 ORF TR2591\|c0_g1_i1\|g.1794 TR2591\|c0_g1_i1\|m.1794 type:complete len:290 (+) TR2591\|c0_g1_i1:53-922(+) [DBVPG6044_S_cerevisiae-Sep2015-contam.fasta] YAL034W-A SGDID:S000000032, Chr I from 79718-80587, Genome Release 64-1-1, Verified ORF, "Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] asmbl_9628\|g.7036 ORF asmbl_9628\|g.7036 asmbl_9628\|m.7036 type:complete len:290 (+) asmbl_9628:45-914(+) [DBVPG6044_Trinity_v2_2_0_PASA_6May2016-contam.fasta] asmbl_9580\|g.6982 ORF asmbl_9580\|g.6982 asmbl_9580\|m.6982 type:complete len:290 (+) asmbl_9580:3353-4222(+) [Y12_Trinity_v2_2_0_PASA_6May2016-contam.fasta] ID=asmbl_9602\|g.5893 ORF ID=asmbl_9602\|g.5893 ID=asmbl_9602\|m.5893 type:complete len:290 (+) ID=asmbl_9602:45-914(+) [DBVPG6044_quiverGenomes_Trinity_v2_2_0_PASA_2June2016-contam.fasta] ID=asmbl_9586\|g.5807 ORF ID=asmbl_9586\|g.5807 ID=asmbl_9586\|m.5807 type:complete len:290 (+) ID=asmbl_9586:3353-4222(+) [Y12_quiver_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	289 amino acids
Gene Ontology:
Cellular Component:	nuclear MIS12/MIND type complex [IDA] spindle pole [IDA]
Biological Process:	chromosome segregation [IDA]
Molecular Function:	molecular_function [ND]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSAPTMRSTS ILTEHLGYPP ISLVDDIINA VNEIMYKCTA AMEKYLLSKS KIGEEDYGEE  60
   61 IKSGVAKLES LLENSVDKNF DKLELYVLRN VLRIPEEYLD ANVFRLENQK DLVIVDENEL 120
  121 KKSEEKLREK VNDVELAFKK NEMLLKRVTK VKRLLFTIRG FKQKLNELLK CKDDVQLQKI 180
  181 LESLKPIDDT MTLLTDSLRK LYVDSESTSS TEEVEALLQR LKTNGKQNNK DFRTRYIDIR 240
  241 TNNVLRKLGL LGDKEDEKQS AKPDARTQAG DIVSIDIEEP QLDLLDDVL

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		DSN1 MTW1 NNF1 NSL1
View Details	Hazbun TR, et al. (2003)		AME1 CHL4 CTF19 CTF3 DSN1 MCM22 MTW1 NKP1 NNF1 NSL1 OKP1
View Details	Krogan NJ, et al. (2006)		DSN1 MTW1 NNF1 NSL1
View Details	Gavin AC, et al. (2006)		AME1 DSN1 MTW1
View Details	Qiu et al. (2008)		DSN1 MTW1 NNF1 NSL1 NUF2 SPC24 SPC25 SPC29

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	NSL1	No Comments	Hazbun TR, et al. (2003)
View Run	CHL4	No Comments	Cheeseman IM, et al. (2002)
View Run	MCM16	No Comments	Cheeseman IM, et al. (2002)
View Run	MIF2	No Comments	Cheeseman IM, et al. (2002)
View Run	DSN1	No Comments	Hazbun TR, et al. (2003)
View Run	NSL1	No Comments	Hazbun TR, et al. (2003)
View Run	DSN1	No Comments	Hazbun TR, et al. (2003)
View Run	NSL1	No Comments	Hazbun TR, et al. (2003)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	SPP382	MTW1	2	Hazbun TR, et al. (2003)

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	MTW1	Huh WK, et al. (2003)
View Data	MTW1 and SPC42	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..218]	Pcons	1.96	Ribonuclease domain of colicin E3; Colicin E3 translocation domain; Colicin E3 receptor domain
2	View Details	[219..289]	Pcons	1.96	Ribonuclease domain of colicin E3; Colicin E3 translocation domain; Colicin E3 receptor domain

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
binding	2.43320979925109	bayes_pls_golite062009
protein binding	1.49557135231286	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2