Bait Protein: | RLF2 |
Organism: | Saccharomyces cerevisiae |
Publication: | Green EM, Antczak AJ, Bailey AO, Franco AA, Wu KJ, Yates JR 3rd, Kaufman PD. Replication-independent histone deposition by the HIR complex and Asf1. Curr Biol. 2005 Nov 22;15(22):2044-9. |
Comments: | cac1: TAP-tagged |
Raw Data: |
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FILTERED Run Results:
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Hit Protein | Protein Desc | Sequence Count | Spectrum Count | Sequence Coverage | NSAF | Mol. Wt. | |
[ |
MSI1 | Subunit of chromatin assembly factor I (CAF-1), negative regulator of the RAS/cAMP pathway via seque... | 29 | 90 | 79.6% | 0.0655 | 47365 |
[ |
CAC2 | Component of the chromatin assembly complex (with Rlf2p and Msi1p) that assembles newly synthesized ... | 39 | 63 | 65.4% | 0.0414 | 51252 |
[ |
TEF1, TEF2 | Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction ... | 29 | 62 | 46.5% | 0.0416 | 50033 |
[ |
ENO2 | Enolase II, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosp... | 11 | 20 | 34.6% | 0.0141 | 46914 |
[ |
ENO1 | Enolase I, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosph... | 10 | 19 | 30.4% | 0.0134 | 46816 |
[ |
RLF2 | Largest subunit (p90) of the Chromatin Assembly Complex (CAF-1) with Cac2p and Msi1p that assembles ... | 10 | 15 | 21.3% | 0.00761 | 70210 |
[ |
CDC19 | Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvat... | 10 | 12 | 25.8% | 0.00738 | 54545 |
[ |
HTA1 | Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of tw... | 7 | 11 | 32.6% | 0.0256 | 13989 |
[ |
HTA2 | Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of tw... | 7 | 11 | 32.6% | 0.0256 | 13989 |
[ |
PDC1 | Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates... | 9 | 10 | 23.3% | 0.00546 | 61495 |
[ |
DED1 | ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all ye... | 9 | 10 | 23.2% | 0.00509 | 65553 |
[ |
GPM1 | Tetrameric phosphoglycerate mutase, mediates the conversion of 3-phosphoglycerate to 2-phosphoglycer... | 7 | 8 | 42.5% | 0.00995 | 27609 |
[ |
ADH1 | Alcohol dehydrogenase, fermentative isozyme active as homo- or heterotetramers; required for the red... | 6 | 7 | 24.4% | 0.00618 | 36849 |
[ |
PMA1 | Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plas... | 5 | 7 | 11.9% | 0.00234 | 99619 |
[ |
HHF1, HHF2 | Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two... | 4 | 6 | 29.1% | 0.0179 | 11368 |
[ |
HHT1, HHT2 | Histone H3, core histone protein required for chromatin assembly, part of heterochromatin-mediated t... | 3 | 6 | 23.5% | 0.0136 | 15356 |
[ |
PGK1 | 3-phosphoglycerate kinase, catalyzes transfer of high-energy phosphoryl groups from the acyl phospha... | 5 | 6 | 13.7% | 0.00443 | 44738 |
[ |
FBA1 | Fructose 1,6-bisphosphate aldolase, required for glycolysis and gluconeogenesis; catalyzes conversio... | 4 | 6 | 13.1% | 0.00514 | 39621 |
[ |
YEF3 | Translational elongation factor 3, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes b... | 6 | 6 | 10.1% | 0.00177 | 115945 |
[ |
POR1 | Mitochondrial porin (voltage-dependent anion channel), outer membrane protein required for the maint... | 5 | 5 | 27.6% | 0.00543 | 30428 |
[ |
RNQ1 | [PIN(+)] prion, an infectious protein conformation that is generally an ordered protein aggregate | 5 | 5 | 24.9% | 0.00379 | 42580 |
[ |
YDR261C-D | Retrotransposon TYA Gag and TYB Pol genes; in YDRCTY1-3 TYB is mutant and probably non-functional | 4 | 5 | 4.8% | 9.58E-4 | 181660 |
[ |
YDR210C-D | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 4 | 5 | 4.4% | 8.76E-4 | 198838 |
[ |
YGR038C-B | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 4 | 5 | 4.4% | 8.76E-4 | 198520 |
[ |
YER160C | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 4 | 5 | 4.4% | 8.76E-4 | 198568 |
[ |
YBR012W-B | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 4 | 5 | 4.4% | 8.75E-4 | 198868 |
[ |
YDR365W-B | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 4 | 5 | 4.4% | 8.76E-4 | 198658 |
[ |
YER138C | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 4 | 5 | 4.4% | 8.76E-4 | 198561 |
[ |
YDR316W-B | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 4 | 5 | 4.4% | 8.76E-4 | 198526 |
[ |
YGR027W-B | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 4 | 5 | 4.4% | 8.76E-4 | 198656 |
[ |
YDR098C-B | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 4 | 5 | 4.4% | 8.76E-4 | 198594 |
[ |
RPP0 | Conserved ribosomal protein P0 similar to rat P0, human P0, and E. coli L10e; shown to be phosphoryl... | 4 | 4 | 13.8% | 0.00394 | 33717 |
[ |
TUB3 | Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form... | 3 | 4 | 12.6% | 0.00276 | 49694 |
[ |
TUB1 | Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form... | 3 | 4 | 10.7% | 0.00275 | 49800 |
[ |
PDC5 | Minor isoform of pyruvate decarboxylase, key enzyme in alcoholic fermentation, decarboxylates pyruva... | 3 | 4 | 7.3% | 0.00218 | 61912 |
[ |
RPP2B | Ribosomal protein P2 beta, a component of the ribosomal stalk, which is involved in the interaction ... | 3 | 3 | 40.9% | 0.00838 | 11050 |
[ |
RIM1 | Single-stranded DNA-binding protein essential for mitochondrial genome maintenance; involved in mito... | 2 | 3 | 30.4% | 0.00683 | 15386 |
[ |
PET9 | Major ADP/ATP carrier of the mitochondrial inner membrane, exchanges cytosolic ADP for mitochondrial... | 3 | 3 | 9.7% | 0.0029 | 34426 |
[ |
ADH2 | Glucose-repressible alcohol dehydrogenase II, catalyzes the conversion of ethanol to acetaldehyde; i... | 3 | 3 | 9.5% | 0.00265 | 36732 |
[ |
YDR210C-C | Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; ... | 2 | 3 | 8.9% | 0.0021 | 49187 |
[ |
YDR365W-A, YPL25... | Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; ... | 2 | 3 | 8.9% | 0.0021 | 48953 |
[ |
YDR261C-C | Retrotransposon TYA Gag gene co-transcribed with TYB Pol; in YDRCTY1-3 TYB is mutant and probably no... | 2 | 3 | 8.9% | 0.0021 | 49009 |
[ |
YPR137C-A, YAR010C | Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; ... | 2 | 3 | 8.9% | 0.0021 | 49072 |
[ |
YBR012W-A | Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; ... | 2 | 3 | 8.9% | 0.0021 | 49068 |
[ |
YGR027W-A | Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; ... | 2 | 3 | 8.9% | 0.0021 | 49056 |
[ |
YER159C-A, YDR31... | Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; ... | 2 | 3 | 8.9% | 0.0021 | 49129 |
[ |
YDR098C-A | Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; ... | 2 | 3 | 8.9% | 0.0021 | 49057 |
[ |
YGR038C-A | Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; ... | 2 | 3 | 8.9% | 0.0021 | 49034 |
[ |
NOP56 | Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that ... | 3 | 3 | 8.3% | 0.00183 | 56864 |
[ |
YDJ1 | Protein chaperone involved in regulation of the HSP90 and HSP70 functions; involved in protein trans... | 2 | 3 | 7.6% | 0.00225 | 44671 |
[ |
PMA2 | Plasma membrane H+-ATPase, isoform of Pma1p, involved in pumping protons out of the cell; regulator ... | 3 | 3 | 6.7% | 9.74E-4 | 102172 |
[ |
PDR1 | Zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins t... | 3 | 3 | 5.5% | 8.63E-4 | 121793 |
[ |
FKS1 | Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic sub... | 3 | 3 | 3.3% | 4.91E-4 | 214849 |
[ |
MIR1 | Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redunda... | 2 | 2 | 16.1% | 0.00198 | 32812 |
[ |
ASF1 | Nucleosome assembly factor, involved in chromatin assembly and disassembly, anti-silencing protein t... | 2 | 2 | 15.4% | 0.0022 | 31603 |
[ |
NOP1 | Nucleolar protein, component of the small subunit processome complex, which is required for processi... | 2 | 2 | 14.4% | 0.00188 | 34465 |
[ |
RVS161 | Amphiphysin-like lipid raft protein; interacts with Rvs167p and regulates polarization of the actin ... | 2 | 2 | 12.1% | 0.00232 | 30250 |
[ |
POL30 | Proliferating cell nuclear antigen (PCNA), functions as the sliding clamp for DNA polymerase delta; ... | 2 | 2 | 11.2% | 0.00238 | 28916 |
[ |
NOP58 | Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the ... | 2 | 2 | 10.2% | 0.0012 | 56956 |
[ |
SSO2 | Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane; syntaxin ho... | 2 | 2 | 10.2% | 0.00208 | 33734 |
[ |
CBF5 | Pseudouridine synthase catalytic subunit of box H/ACA small nucleolar ribonucleoprotein particles (s... | 2 | 2 | 9.5% | 0.00127 | 54705 |
[ |
RPC40 | RNA polymerase subunit AC40, common to RNA polymerase I and III | 2 | 2 | 9.0% | 0.00183 | 37687 |
[ |
TRM7 | 2'-O-ribose methyltransferase, methylates the 2'-O-ribose of nucleotides at positions 32 and 34 of t... | 2 | 2 | 8.7% | 0.00198 | 34701 |
[ |
NSR1 | Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ri... | 2 | 2 | 8.5% | 0.00148 | 44535 |
[ |
CYS4 | Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the ... | 2 | 2 | 7.5% | 0.00121 | 56022 |
[ |
VMA2 | Subunit B of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), a... | 2 | 2 | 7.2% | 0.00119 | 57749 |
[ |
ACO1 | Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitoc... | 2 | 2 | 5.8% | 7.9E-4 | 85368 |
[ |
EFT2, EFT1 | Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein s... | 2 | 2 | 5.0% | 7.3E-4 | 93289 |
[ |
RVB2 | Essential protein involved in transcription regulation; component of chromatin remodeling complexes;... | 2 | 2 | 4.9% | 0.00131 | 51612 |
[ |
SUM1 | Transcriptional repressor required for mitotic repression of middle sporulation-specific genes; also... | 2 | 2 | 4.7% | 5.79E-4 | 118201 |
[ |
TFP1 | Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein prec... | 2 | 2 | 4.3% | 5.74E-4 | 118637 |
[ |
HSC82 | Cytoplasmic chaperone of the Hsp90 family, redundant in function and nearly identical with Hsp82p, a... | 2 | 2 | 4.3% | 8.72E-4 | 80900 |
[ |
HSP82 | Hsp90 chaperone required for pheromone signaling and negative regulation of Hsf1p; docks with Tom70p... | 2 | 2 | 4.2% | 8.67E-4 | 81406 |
[ |
GSY1 | Glycogen synthase with similarity to Gsy2p, the more highly expressed yeast homolog; expression indu... | 2 | 2 | 4.2% | 8.68E-4 | 80510 |
[ |
TCB2 | Bud-specific protein with a potential role in membrane trafficking; GFP-fusion protein migrates from... | 2 | 2 | 3.7% | 5.22E-4 | 132509 |
[ |
TCB1 | Lipid-binding protein containing three calcium and lipid binding domains; non-tagged protein localiz... | 2 | 2 | 3.5% | 5.18E-4 | 133576 |
[ |
YLR035C-A | Retrotransposon TYA Gag and TYB Pol genes; polyprotein is processed to make a nucleocapsid-like prot... | 2 | 2 | 3.3% | 5.32E-4 | 131617 |
[ |
YAR009C | Retrotransposon TYA Gag and TYB Pol genes; in YARCTY1-1 TYB is mutant and probably non-functional | 2 | 2 | 3.2% | 5.14E-4 | 136732 |
[ |
SAP185 | Protein that forms a complex with the Sit4p protein phosphatase and is required for its function; me... | 2 | 2 | 3.2% | 5.81E-4 | 121403 |
[ |
FUN12 | GTPase, required for general translation initiation by promoting Met-tRNAiMet binding to ribosomes a... | 2 | 2 | 2.9% | 6.13E-4 | 112268 |
[ |
YLR157C-B | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 2 | 2 | 2.2% | 3.5E-4 | 198544 |
[ |
YGR161C-D | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 2 | 2 | 2.2% | 3.5E-4 | 198558 |
[ |
YML045W | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 2 | 2 | 2.2% | 3.5E-4 | 198524 |
[ |
YJR027W | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 2 | 2 | 2.2% | 3.5E-4 | 198651 |
[ |
YBL005W-B | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 2 | 2 | 2.2% | 3.5E-4 | 198968 |
[ |
YML039W | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 2 | 2 | 2.2% | 3.5E-4 | 198571 |
[ |
YJR029W | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 2 | 2 | 2.2% | 3.5E-4 | 198616 |
[ |
YHR214C-B | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 2 | 2 | 2.1% | 3.43E-4 | 202816 |
[ |
STH1 | ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic g... | 2 | 2 | 2.1% | 4.52E-4 | 156742 |
[ |
GCN1 | Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn20p; proposed to stimulate ... | 2 | 2 | 1.6% | 2.3E-4 | 296697 |
[ |
CAT8 | Zinc cluster transcriptional activator necessary for derepression of a variety of genes under non-fe... | 2 | 2 | 1.4% | 4.29E-4 | 160485 |