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Bait Protein: MLP2
Organism: Saccharomyces cerevisiae
Publication: Keck JM, Jones MH, Wong CC, Binkley J, Chen D, Jaspersen SL, Holinger EP, Xu T, Niepel M, Rout MP, Vogel J, Sidow A, Yates JR, Winey M., A Cell Cycle Phosphoproteome of the Yeast Centrosome, Science 24 June 2011: 1557-1561.
Comments: #08 Alpha Factor Prep4-TiO2 Phosphopeptide enrichment

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FILTERED Run Results: [unfilter]

[View our MS interpretation guidelines.]
  Hit Protein Protein Desc Sequence Count Spectrum Count Sequence Coverage NSAF Mol. Wt.
[View Peptides]   YIL002W-A Putative protein of unknown function; identified by expression profiling and mass spectrometry 21 74 98.6% 0.136 7729
[View Peptides]   MLP2 Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... 542 2073 93.7% 0.156 195140
[View Peptides]   SPC42 Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate att... 28 61 65.6% 0.0212 42271
[View Peptides]   CMD1 Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, act... 5 7 58.5% 0.00602 16135
[View Peptides]   TEF1, TEF2 Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction ... 23 52 56.8% 0.0144 50033
[View Peptides]   CDC19 Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvat... 21 49 48.8% 0.0124 54545
[View Peptides]   CNM67 Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclea... 20 35 44.4% 0.00762 67400
[View Peptides]   CDC31 Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication i... 7 19 44.1% 0.0149 18751
[View Peptides]   SPC29 Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inn... 12 29 43.1% 0.0145 29280
[View Peptides]   GPM1 Tetrameric phosphoglycerate mutase, mediates the conversion of 3-phosphoglycerate to 2-phosphoglycer... 3 4 38.9% 0.00205 27609
[View Peptides]   VMA2 Subunit B of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), a... 12 23 38.1% 0.00563 57749
[View Peptides]   ENO2 Enolase II, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosp... 10 18 37.5% 0.00521 46914
[View Peptides]   NUD1 Component of the spindle pole body outer plaque, required for exit from mitosis 17 26 37.3% 0.00386 94104
[View Peptides]   PDC1 Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates... 12 17 33.2% 0.00382 61495
[View Peptides]   SAM1 S-adenosylmethionine synthetase, catalyzes transfer of the adenosyl group of ATP to the sulfur atom ... 7 8 32.7% 0.00265 41818
[View Peptides]   ACT1 Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functio... 6 8 31.2% 0.0027 41690
[View Peptides]   AHP1 Thiol-specific peroxiredoxin, reduces hydroperoxides to protect against oxidative damage; function i... 2 2 29.0% 0.00144 19115
[View Peptides]   ASC1 G-protein beta subunit and guanine nucleotide dissociation inhibitor for Gpa2p; ortholog of RACK1 th... 5 10 27.9% 0.00396 34805
[View Peptides]   NOP1 Nucleolar protein, component of the small subunit processome complex, which is required for processi... 8 13 27.5% 0.00503 34465
[View Peptides]   PSA1 GDP-mannose pyrophosphorylase (mannose-1-phosphate guanyltransferase), synthesizes GDP-mannose from ... 7 14 27.1% 0.0049 39566
[View Peptides]   TPI1 Triose phosphate isomerase, abundant glycolytic enzyme; mRNA half-life is regulated by iron availabi... 3 5 26.6% 0.00255 26795
[View Peptides]   FBA1 Fructose 1,6-bisphosphate aldolase, required for glycolysis and gluconeogenesis; catalyzes conversio... 6 15 25.9% 0.00528 39621
[View Peptides]   PGK1 3-phosphoglycerate kinase, catalyzes transfer of high-energy phosphoryl groups from the acyl phospha... 6 9 24.3% 0.00274 44738
[View Peptides]   ADH1 Alcohol dehydrogenase, fermentative isozyme active as homo- or heterotetramers; required for the red... 6 11 24.1% 0.004 36849
[View Peptides]   NPL3 RNA-binding protein that promotes elongation, regulates termination, and carries poly(A) mRNA from n... 5 8 22.5% 0.00244 45407
[View Peptides]   SPC110 Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nu... 19 30 21.5% 0.00402 111782
[View Peptides]   YHB1 Nitric oxide oxidoreductase, flavohemoglobin involved in nitric oxide detoxification; plays a role i... 4 4 19.5% 0.00127 44646
[View Peptides]   SNU13 RNA binding protein, part of U3 snoRNP involved in rRNA processing, part of U4/U6-U5 tri-snRNP invol... 2 4 19.0% 0.00401 13569
[View Peptides]   EFT2, EFT1 Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein s... 8 10 17.8% 0.0015 93289
[View Peptides]   RNQ1 [PIN(+)] prion, an infectious protein conformation that is generally an ordered protein aggregate 4 5 16.3% 0.00156 42580
[View Peptides]   BFA1 Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pa... 5 6 16.2% 0.00132 66087
[View Peptides]   DEF1 RNAPII degradation factor, forms a complex with Rad26p in chromatin, enables ubiquitination and prot... 11 36 15.0% 0.00617 83973
[View Peptides]   GFA1 Glutamine-fructose-6-phosphate amidotransferase, catalyzes the formation of glucosamine-6-P and glut... 5 12 14.6% 0.00212 80047
[View Peptides]   TUB1 Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form... 2 3 13.9% 8.49E-4 49800
[View Peptides]   NOP58 Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the ... 4 9 12.7% 0.00223 56956
[View Peptides]   DED1 ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all ye... 4 5 12.6% 0.00105 65553
[View Peptides]   ASP1 Cytosolic L-asparaginase, involved in asparagine catabolism 2 2 11.3% 6.64E-4 41395
[View Peptides]   HSP42 Small heat shock protein (sHSP) with chaperone activity; forms barrel-shaped oligomers that suppress... 2 3 10.1% 0.00101 42817
[View Peptides]   ILV1 Threonine deaminase, catalyzes the first step in isoleucine biosynthesis; expression is under genera... 3 3 9.4% 6.59E-4 63831
[View Peptides]   ACS2 Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histo... 3 3 7.3% 5.55E-4 75492
[View Peptides]   BGL2 Endo-beta-1,3-glucanase, major protein of the cell wall, involved in cell wall maintenance 2 2 7.3% 8.08E-4 34119
[View Peptides]   PMA1 Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plas... 5 7 7.0% 9.64E-4 99619
[View Peptides]   PMA2 Plasma membrane H+-ATPase, isoform of Pma1p, involved in pumping protons out of the cell; regulator ... 5 7 6.8% 9.35E-4 102172
[View Peptides]   BBP1 Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periph... 2 2 6.8% 6.57E-4 45384
[View Peptides]   NOP56 Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that ... 2 2 6.7% 5.02E-4 56864
[View Peptides]   MLP1 Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... 6 12 5.8% 8.09E-4 218454
[View Peptides]   GRS1 Cytoplasmic and mitochondrial glycyl-tRNA synthase that ligates glycine to the cognate anticodon bea... 2 2 5.1% 3.79E-4 75411
[View Peptides]   YDR365W-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 4 4 4.1% 2.88E-4 198658
[View Peptides]   YDR210C-D Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 4 4 4.1% 2.88E-4 198838
[View Peptides]   YDR316W-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 4 4 4.1% 2.88E-4 198526
[View Peptides]   YGR038C-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 4 4 4.1% 2.88E-4 198520
[View Peptides]   YDR098C-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 4 4 4.1% 2.88E-4 198594
[View Peptides]   YER138C Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 4 4 4.1% 2.88E-4 198561
[View Peptides]   FAS2 Alpha subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty ... 2 2 1.7% 1.34E-4 206945
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