Bait Protein: | MLP2 |
Organism: | Saccharomyces cerevisiae |
Publication: | Keck JM, Jones MH, Wong CC, Binkley J, Chen D, Jaspersen SL, Holinger EP, Xu T, Niepel M, Rout MP, Vogel J, Sidow A, Yates JR, Winey M., A Cell Cycle Phosphoproteome of the Yeast Centrosome, Science 24 June 2011: 1557-1561. |
Comments: | #02 Alpha Factor Prep1-TiO2 enriched, new search criteria |
Raw Data: |
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FILTERED Run Results:
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Hit Protein | Protein Desc | Sequence Count | Spectrum Count | Sequence Coverage | NSAF | Mol. Wt. | |
[ |
YIL002W-A | Putative protein of unknown function; identified by expression profiling and mass spectrometry | 14 | 15 | 98.6% | 0.175 | 7729 |
[ |
MLP2 | Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... | 144 | 177 | 57.6% | 0.0847 | 195140 |
[ |
CDC31 | Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication i... | 10 | 10 | 51.6% | 0.0499 | 18751 |
[ |
CNM67 | Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclea... | 20 | 20 | 45.6% | 0.0277 | 67400 |
[ |
SPC110 | Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nu... | 50 | 58 | 45.3% | 0.0494 | 111782 |
[ |
RPP2B | Ribosomal protein P2 beta, a component of the ribosomal stalk, which is involved in the interaction ... | 2 | 2 | 43.6% | 0.0146 | 11050 |
[ |
SPC42 | Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate att... | 19 | 21 | 42.1% | 0.0465 | 42271 |
[ |
TUB4 | Gamma-tubulin, involved in nucleating microtubules from both the cytoplasmic and nuclear faces of th... | 6 | 6 | 31.1% | 0.0102 | 52628 |
[ |
BBP1 | Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periph... | 6 | 6 | 20.0% | 0.0125 | 45384 |
[ |
SPC29 | Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inn... | 7 | 8 | 18.6% | 0.0254 | 29280 |
[ |
NBP1 | Spindle pole body (SPB) component, required for the insertion of the duplication plaque into the nuc... | 3 | 3 | 16.3% | 0.00756 | 37354 |
[ |
CDC19 | Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvat... | 6 | 6 | 14.6% | 0.00964 | 54545 |
[ |
HSL7 | Protein arginine N-methyltransferase that exhibits septin and Hsl1p-dependent bud neck localization ... | 8 | 8 | 13.2% | 0.00777 | 95153 |
[ |
KAR1 | Essential protein involved in karyogamy during mating and in spindle pole body duplication during mi... | 3 | 3 | 12.2% | 0.00557 | 50653 |
[ |
MPS2 | Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required f... | 4 | 4 | 12.1% | 0.00831 | 44585 |
[ |
BFA1 | Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pa... | 4 | 4 | 11.7% | 0.0056 | 66087 |
[ |
SPC97 | Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the... | 5 | 14 | 11.1% | 0.0137 | 96825 |
[ |
NUD1 | Component of the spindle pole body outer plaque, required for exit from mitosis | 7 | 7 | 10.8% | 0.00661 | 94104 |
[ |
NOP1 | Nucleolar protein, component of the small subunit processome complex, which is required for processi... | 2 | 2 | 10.4% | 0.00492 | 34465 |
[ |
DEF1 | RNAPII degradation factor, forms a complex with Rad26p in chromatin, enables ubiquitination and prot... | 6 | 7 | 9.8% | 0.00762 | 83973 |
[ |
ACS2 | Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histo... | 4 | 4 | 9.2% | 0.00471 | 75492 |
[ |
CMD1 | Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, act... | 2 | 2 | 8.8% | 0.0109 | 16135 |
[ |
NPL3 | RNA-binding protein that promotes elongation, regulates termination, and carries poly(A) mRNA from n... | 2 | 2 | 8.7% | 0.00388 | 45407 |
[ |
SPC98 | Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the... | 3 | 7 | 7.8% | 0.00665 | 98227 |
[ |
NOP58 | Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the ... | 2 | 2 | 7.6% | 0.00315 | 56956 |
[ |
DED1 | ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all ye... | 3 | 3 | 6.6% | 0.00399 | 65553 |
[ |
GFA1 | Glutamine-fructose-6-phosphate amidotransferase, catalyzes the formation of glucosamine-6-P and glut... | 2 | 3 | 5.9% | 0.00336 | 80047 |
[ |
KAR2 | ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding i... | 2 | 2 | 5.9% | 0.00236 | 74468 |
[ |
PMA1 | Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plas... | 5 | 6 | 5.4% | 0.00525 | 99619 |
[ |
MSH6 | Protein required for mismatch repair in mitosis and meiosis, forms a complex with Msh2p to repair bo... | 2 | 2 | 4.8% | 0.00129 | 140080 |
[ |
MLP1 | Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... | 5 | 5 | 4.6% | 0.00214 | 218454 |
[ |
SWI4 | DNA binding component of the SBF complex (Swi4p-Swi6p), a transcriptional activator that in concert ... | 2 | 2 | 3.4% | 0.00147 | 123806 |
[ |
SFI1 | Centrin (Cdc31p)-binding protein required for spindle pole body (SPB) duplication, localizes to the ... | 2 | 2 | 3.1% | 0.0017 | 112978 |
[ |
FUN12 | GTPase, required for general translation initiation by promoting Met-tRNAiMet binding to ribosomes a... | 2 | 2 | 3.0% | 0.0016 | 112268 |
[ |
URA2 | Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes t... | 4 | 4 | 2.7% | 0.00145 | 245123 |
[ |
MRC1 | S-phase checkpoint protein required for DNA replication; interacts with and stabilizes Pol2p at stal... | 2 | 2 | 2.7% | 0.00147 | 124326 |
[ |
SKG3 | Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell pe... | 2 | 2 | 2.4% | 0.00157 | 114111 |
[ |
PMD1 | Protein with an N-terminal kelch-like domain, putative negative regulator of early meiotic gene expr... | 2 | 2 | 1.8% | 9.17E-4 | 195382 |
[ |
YJR029W | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 2 | 2 | 1.1% | 9.16E-4 | 198616 |
[ |
YGR027W-B | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 2 | 2 | 1.1% | 9.16E-4 | 198656 |
[ |
YPR158C-D | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 2 | 2 | 1.1% | 9.16E-4 | 198373 |
[ |
MDN1 | Huge dynein-related AAA-type ATPase (midasin), forms extended pre-60S particle with the Rix1 complex... | 2 | 2 | 1.0% | 3.27E-4 | 559316 |