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Bait Protein: MLP2
Organism: Saccharomyces cerevisiae
Publication: Keck JM, Jones MH, Wong CC, Binkley J, Chen D, Jaspersen SL, Holinger EP, Xu T, Niepel M, Rout MP, Vogel J, Sidow A, Yates JR, Winey M., A Cell Cycle Phosphoproteome of the Yeast Centrosome, Science 24 June 2011: 1557-1561.
Comments: #02 Alpha Factor Prep1-TiO2 enriched, new search criteria

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  Hit Protein Protein Desc Sequence Count Spectrum Count Sequence Coverage NSAF Mol. Wt.
[View Peptides]   YIL002W-A Putative protein of unknown function; identified by expression profiling and mass spectrometry 14 15 98.6% 0.175 7729
[View Peptides]   MLP2 Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... 144 177 57.6% 0.0847 195140
[View Peptides]   CDC31 Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication i... 10 10 51.6% 0.0499 18751
[View Peptides]   CNM67 Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclea... 20 20 45.6% 0.0277 67400
[View Peptides]   SPC110 Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nu... 50 58 45.3% 0.0494 111782
[View Peptides]   RPP2B Ribosomal protein P2 beta, a component of the ribosomal stalk, which is involved in the interaction ... 2 2 43.6% 0.0146 11050
[View Peptides]   SPC42 Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate att... 19 21 42.1% 0.0465 42271
[View Peptides]   TUB4 Gamma-tubulin, involved in nucleating microtubules from both the cytoplasmic and nuclear faces of th... 6 6 31.1% 0.0102 52628
[View Peptides]   BBP1 Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periph... 6 6 20.0% 0.0125 45384
[View Peptides]   SPC29 Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inn... 7 8 18.6% 0.0254 29280
[View Peptides]   NBP1 Spindle pole body (SPB) component, required for the insertion of the duplication plaque into the nuc... 3 3 16.3% 0.00756 37354
[View Peptides]   CDC19 Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvat... 6 6 14.6% 0.00964 54545
[View Peptides]   HSL7 Protein arginine N-methyltransferase that exhibits septin and Hsl1p-dependent bud neck localization ... 8 8 13.2% 0.00777 95153
[View Peptides]   KAR1 Essential protein involved in karyogamy during mating and in spindle pole body duplication during mi... 3 3 12.2% 0.00557 50653
[View Peptides]   MPS2 Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required f... 4 4 12.1% 0.00831 44585
[View Peptides]   BFA1 Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pa... 4 4 11.7% 0.0056 66087
[View Peptides]   SPC97 Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the... 5 14 11.1% 0.0137 96825
[View Peptides]   NUD1 Component of the spindle pole body outer plaque, required for exit from mitosis 7 7 10.8% 0.00661 94104
[View Peptides]   NOP1 Nucleolar protein, component of the small subunit processome complex, which is required for processi... 2 2 10.4% 0.00492 34465
[View Peptides]   DEF1 RNAPII degradation factor, forms a complex with Rad26p in chromatin, enables ubiquitination and prot... 6 7 9.8% 0.00762 83973
[View Peptides]   ACS2 Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histo... 4 4 9.2% 0.00471 75492
[View Peptides]   CMD1 Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, act... 2 2 8.8% 0.0109 16135
[View Peptides]   NPL3 RNA-binding protein that promotes elongation, regulates termination, and carries poly(A) mRNA from n... 2 2 8.7% 0.00388 45407
[View Peptides]   SPC98 Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the... 3 7 7.8% 0.00665 98227
[View Peptides]   NOP58 Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the ... 2 2 7.6% 0.00315 56956
[View Peptides]   DED1 ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all ye... 3 3 6.6% 0.00399 65553
[View Peptides]   GFA1 Glutamine-fructose-6-phosphate amidotransferase, catalyzes the formation of glucosamine-6-P and glut... 2 3 5.9% 0.00336 80047
[View Peptides]   KAR2 ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding i... 2 2 5.9% 0.00236 74468
[View Peptides]   PMA1 Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plas... 5 6 5.4% 0.00525 99619
[View Peptides]   MSH6 Protein required for mismatch repair in mitosis and meiosis, forms a complex with Msh2p to repair bo... 2 2 4.8% 0.00129 140080
[View Peptides]   MLP1 Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... 5 5 4.6% 0.00214 218454
[View Peptides]   SWI4 DNA binding component of the SBF complex (Swi4p-Swi6p), a transcriptional activator that in concert ... 2 2 3.4% 0.00147 123806
[View Peptides]   SFI1 Centrin (Cdc31p)-binding protein required for spindle pole body (SPB) duplication, localizes to the ... 2 2 3.1% 0.0017 112978
[View Peptides]   FUN12 GTPase, required for general translation initiation by promoting Met-tRNAiMet binding to ribosomes a... 2 2 3.0% 0.0016 112268
[View Peptides]   URA2 Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes t... 4 4 2.7% 0.00145 245123
[View Peptides]   MRC1 S-phase checkpoint protein required for DNA replication; interacts with and stabilizes Pol2p at stal... 2 2 2.7% 0.00147 124326
[View Peptides]   SKG3 Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell pe... 2 2 2.4% 0.00157 114111
[View Peptides]   PMD1 Protein with an N-terminal kelch-like domain, putative negative regulator of early meiotic gene expr... 2 2 1.8% 9.17E-4 195382
[View Peptides]   YJR029W Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 2 2 1.1% 9.16E-4 198616
[View Peptides]   YGR027W-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 2 2 1.1% 9.16E-4 198656
[View Peptides]   YPR158C-D Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 2 2 1.1% 9.16E-4 198373
[View Peptides]   MDN1 Huge dynein-related AAA-type ATPase (midasin), forms extended pre-60S particle with the Rix1 complex... 2 2 1.0% 3.27E-4 559316

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