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Bait Protein: MLP2
Organism: Saccharomyces cerevisiae
Publication: Keck JM, Jones MH, Wong CC, Binkley J, Chen D, Jaspersen SL, Holinger EP, Xu T, Niepel M, Rout MP, Vogel J, Sidow A, Yates JR, Winey M., A Cell Cycle Phosphoproteome of the Yeast Centrosome, Science 24 June 2011: 1557-1561.
Comments: #31 Asynchronous Prep (Protease cleavage)

Raw Data:

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FILTERED Run Results: [unfilter]

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  Hit Protein Protein Desc Sequence Count Spectrum Count Sequence Coverage NSAF Mol. Wt.
[View Peptides]   RPP1B Ribosomal protein P1 beta, component of the ribosomal stalk, which is involved in interaction of tra... 5 18 64.2% 0.0492 10668
[View Peptides]   SEM1 Component of the lid subcomplex of the regulatory subunit of the 26S proteasome; involved in mRNA ex... 3 11 62.9% 0.0358 10386
[View Peptides]   CMD1 Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, act... 3 8 55.1% 0.0158 16135
[View Peptides]   RPP1A Ribosomal stalk protein P1 alpha, involved in the interaction between translational elongation facto... 3 16 48.1% 0.0438 10908
[View Peptides]   RPP2B Ribosomal protein P2 beta, a component of the ribosomal stalk, which is involved in the interaction ... 3 22 43.6% 0.058 11050
[View Peptides]   RPP2A Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction... 4 14 43.4% 0.0383 10746
[View Peptides]   YIL002W-A Putative protein of unknown function; identified by expression profiling and mass spectrometry 2 3 31.9% 0.0126 7729
[View Peptides]   CDC31 Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication i... 3 6 30.4% 0.0108 18751
[View Peptides]   SPC42 Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate att... 9 27 26.7% 0.0216 42271
[View Peptides]   PSA1 GDP-mannose pyrophosphorylase (mannose-1-phosphate guanyltransferase), synthesizes GDP-mannose from ... 6 11 22.7% 0.00884 39566
[View Peptides]   MLP2 Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... 31 75 22.2% 0.013 195140
[View Peptides]   YDJ1 Protein chaperone involved in regulation of the HSP90 and HSP70 functions; involved in protein trans... 2 65 19.1% 0.0461 44671
[View Peptides]   CNM67 Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclea... 9 26 17.6% 0.013 67400
[View Peptides]   CDC19 Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvat... 6 33 17.0% 0.0191 54545
[View Peptides]   SAM1 S-adenosylmethionine synthetase, catalyzes transfer of the adenosyl group of ATP to the sulfur atom ... 3 22 15.7% 0.0167 41818
[View Peptides]   RPN8 Essential, non-ATPase regulatory subunit of the 26S proteasome; has similarity to the human p40 prot... 2 10 13.9% 0.00858 38313
[View Peptides]   SAM2 S-adenosylmethionine synthetase, catalyzes transfer of the adenosyl group of ATP to the sulfur atom ... 2 12 12.8% 0.00906 42256
[View Peptides]   RAD51 Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in t... 2 2 12.5% 0.00145 42963
[View Peptides]   RPT5 One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation ... 2 3 11.1% 0.002 48256
[View Peptides]   SPC110 Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nu... 10 28 11.0% 0.0086 111782
[View Peptides]   YBR287W Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the ER; YBR... 2 2 11.0% 0.00136 47506
[View Peptides]   CPR6 Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide ... 2 15 10.2% 0.0117 42072
[View Peptides]   BFA1 Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pa... 3 5 9.9% 0.00253 66087
[View Peptides]   SBP1 Putative RNA binding protein; involved in translational repression and found in cytoplasmic P bodies... 2 53 9.9% 0.0523 32990
[View Peptides]   KAR2 ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding i... 6 21 9.1% 0.00893 74468
[View Peptides]   TEF1, TEF2 Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction ... 2 2 8.7% 0.00127 50033
[View Peptides]   YOR051C Nuclear protein that inhibits replication of Brome mosaic virus in S. cerevisiae, which is a model s... 2 6 8.7% 0.00422 47352
[View Peptides]   URA7 Major CTP synthase isozyme (see also URA8), catalyzes the ATP-dependent transfer of the amide nitrog... 2 17 8.6% 0.00851 64710
[View Peptides]   CCT5 Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of... 2 3 8.4% 0.00155 61914
[View Peptides]   NFU1 Protein involved in iron metabolism in mitochondria; similar to NifU, which is a protein required fo... 2 4 8.2% 0.00453 29174
[View Peptides]   DEF1 RNAPII degradation factor, forms a complex with Rad26p in chromatin, enables ubiquitination and prot... 2 19 7.9% 0.00747 83973
[View Peptides]   HSC82 Cytoplasmic chaperone of the Hsp90 family, redundant in function and nearly identical with Hsp82p, a... 3 3 7.9% 0.00123 80900
[View Peptides]   EFT2, EFT1 Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein s... 4 8 7.8% 0.00276 93289
[View Peptides]   RPN7 Essential, non-ATPase regulatory subunit of the 26S proteasome, similar to another S. cerevisiae reg... 2 24 7.7% 0.0162 48959
[View Peptides]   PGK1 3-phosphoglycerate kinase, catalyzes transfer of high-energy phosphoryl groups from the acyl phospha... 2 2 7.7% 0.00139 44738
[View Peptides]   PDI1 Protein disulfide isomerase, multifunctional protein resident in the endoplasmic reticulum lumen, es... 4 16 7.5% 0.00889 58227
[View Peptides]   NBP1 Spindle pole body (SPB) component, required for the insertion of the duplication plaque into the nuc... 2 4 7.5% 0.00364 37354
[View Peptides]   CDC48 ATPase in ER, nuclear membrane and cytosol with homology to mammalian p97; in a complex with Npl4p a... 3 24 6.9% 0.00834 91996
[View Peptides]   ACT1 Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functio... 2 6 6.9% 0.00464 41690
[View Peptides]   GRS1 Cytoplasmic and mitochondrial glycyl-tRNA synthase that ligates glycine to the cognate anticodon bea... 3 5 6.7% 0.00217 75411
[View Peptides]   SPC29 Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inn... 3 9 6.3% 0.0103 29280
[View Peptides]   ARB1 ATPase of the ATP-binding cassette (ABC) family involved in 40S and 60S ribosome biogenesis, has sim... 2 2 6.2% 9.51E-4 68377
[View Peptides]   HSP42 Small heat shock protein (sHSP) with chaperone activity; forms barrel-shaped oligomers that suppress... 2 4 6.1% 0.00309 42817
[View Peptides]   GFA1 Glutamine-fructose-6-phosphate amidotransferase, catalyzes the formation of glucosamine-6-P and glut... 2 5 6.0% 0.00202 80047
[View Peptides]   CPA2 Large subunit of carbamoyl phosphate synthetase, which catalyzes a step in the synthesis of citrulli... 5 9 5.5% 0.00233 123915
[View Peptides]   NUD1 Component of the spindle pole body outer plaque, required for exit from mitosis 2 2 5.4% 6.82E-4 94104
[View Peptides]   NPL3 RNA-binding protein that promotes elongation, regulates termination, and carries poly(A) mRNA from n... 2 3 5.3% 0.0021 45407
[View Peptides]   ADE4 Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes fir... 2 4 4.9% 0.00227 56719
[View Peptides]   CHC1 Clathrin heavy chain, subunit of the major coat protein involved in intracellular protein transport ... 3 4 4.7% 7.02E-4 187233
[View Peptides]   MAK21 Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; i... 2 4 4.0% 0.00113 116676
[View Peptides]   SSC1 Hsp70 family ATPase, constituent of the import motor component of the Translocase of the Inner Mitoc... 2 7 4.0% 0.0031 70628
[View Peptides]   YHR020W Protein of unknown function that may interact with ribosomes, based on co-purification experiments; ... 2 6 3.9% 0.00253 77386
[View Peptides]   YEF3 Translational elongation factor 3, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes b... 2 25 3.3% 0.00694 115945
[View Peptides]   FAS2 Alpha subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty ... 3 32 3.2% 0.00492 206945
[View Peptides]   SUP35 Translation termination factor eRF3; altered protein conformation creates the [PSI(+)] prion, a domi... 2 7 2.6% 0.00296 76551
[View Peptides]   FAS1 Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty a... 2 4 2.0% 5.66E-4 228689
[View Peptides]   URA2 Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes t... 3 6 1.9% 7.86E-4 245123
[View Peptides]   ACC1 Acetyl-CoA carboxylase, biotin containing enzyme that catalyzes the carboxylation of acetyl-CoA to f... 3 4 1.4% 5.19E-4 250351

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