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Bait Protein: MLP2
Organism: Saccharomyces cerevisiae
Publication: Keck JM, Jones MH, Wong CC, Binkley J, Chen D, Jaspersen SL, Holinger EP, Xu T, Niepel M, Rout MP, Vogel J, Sidow A, Yates JR, Winey M., A Cell Cycle Phosphoproteome of the Yeast Centrosome, Science 24 June 2011: 1557-1561.
Comments: #34 Asynchronous SPB prep

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  Hit Protein Protein Desc Sequence Count Spectrum Count Sequence Coverage NSAF Mol. Wt.
[View Peptides]   CMD1 Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, act... 11 20 48.3% 0.0998 16135
[View Peptides]   CDC31 Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication i... 8 16 47.2% 0.0729 18751
[View Peptides]   BFA1 Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pa... 17 37 28.2% 0.0473 66087
[View Peptides]   NUD1 Component of the spindle pole body outer plaque, required for exit from mitosis 28 47 23.1% 0.0405 94104
[View Peptides]   SPC42 Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate att... 8 13 22.9% 0.0263 42271
[View Peptides]   MLP2 Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... 42 64 17.8% 0.028 195140
[View Peptides]   SPC110 Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nu... 22 61 17.2% 0.0474 111782
[View Peptides]   SPC29 Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inn... 8 13 15.0% 0.0377 29280
[View Peptides]   CDC14 Protein phosphatase required for mitotic exit; located in the nucleolus until liberated by the FEAR ... 11 18 14.7% 0.024 61907
[View Peptides]   TEF1, TEF2 Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction ... 5 8 12.7% 0.0128 50033
[View Peptides]   CNM67 Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclea... 8 19 11.9% 0.024 67400
[View Peptides]   NBP1 Spindle pole body (SPB) component, required for the insertion of the duplication plaque into the nuc... 3 5 11.6% 0.0115 37354
[View Peptides]   BBP1 Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periph... 2 5 8.3% 0.00953 45384
[View Peptides]   AIF1 Mitochondrial cell death effector that translocates to the nucleus in response to apoptotic stimuli,... 2 2 7.9% 0.00388 41335
[View Peptides]   TUB4 Gamma-tubulin, involved in nucleating microtubules from both the cytoplasmic and nuclear faces of th... 4 12 6.3% 0.0186 52628
[View Peptides]   SPC72 Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them... 3 4 6.1% 0.00472 72105
[View Peptides]   VMA2 Subunit B of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), a... 2 2 5.4% 0.00284 57749
[View Peptides]   SPC97 Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the... 2 2 3.3% 0.00178 96825
[View Peptides]   SPC98 Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the... 2 2 2.8% 0.00173 98227
[View Peptides]   EFT2, EFT1 Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein s... 3 3 2.6% 0.00261 93289

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