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Bait Protein: MLP2
Organism: Saccharomyces cerevisiae
Publication: Keck JM, Jones MH, Wong CC, Binkley J, Chen D, Jaspersen SL, Holinger EP, Xu T, Niepel M, Rout MP, Vogel J, Sidow A, Yates JR, Winey M., A Cell Cycle Phosphoproteome of the Yeast Centrosome, Science 24 June 2011: 1557-1561.
Comments: #26 Asynchronous Prep4-TiO2 Phosphopeptide enriched, Steps1-2

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FILTERED Run Results: [unfilter]

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  Hit Protein Protein Desc Sequence Count Spectrum Count Sequence Coverage NSAF Mol. Wt.
[View Peptides]   YIL002W-A Putative protein of unknown function; identified by expression profiling and mass spectrometry 10 11 98.6% 0.0442 7729
[View Peptides]   CDC31 Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication i... 22 36 95.7% 0.0619 18751
[View Peptides]   SPC42 Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate att... 104 210 92.8% 0.16 42271
[View Peptides]   SPC29 Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inn... 51 98 88.5% 0.107 29280
[View Peptides]   CMD1 Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, act... 9 18 85.7% 0.0339 16135
[View Peptides]   SPC110 Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nu... 199 377 83.3% 0.111 111782
[View Peptides]   BFA1 Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pa... 47 72 67.2% 0.0347 66087
[View Peptides]   CNM67 Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclea... 74 122 65.1% 0.0582 67400
[View Peptides]   NBP1 Spindle pole body (SPB) component, required for the insertion of the duplication plaque into the nuc... 12 12 46.7% 0.0104 37354
[View Peptides]   MLP2 Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... 83 115 45.3% 0.019 195140
[View Peptides]   BBP1 Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periph... 14 20 44.4% 0.0144 45384
[View Peptides]   NUD1 Component of the spindle pole body outer plaque, required for exit from mitosis 62 117 41.2% 0.0381 94104
[View Peptides]   CDC14 Protein phosphatase required for mitotic exit; located in the nucleolus until liberated by the FEAR ... 19 28 35.8% 0.0141 61907
[View Peptides]   MPS2 Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required f... 12 15 31.0% 0.0107 44585
[View Peptides]   KAR1 Essential protein involved in karyogamy during mating and in spindle pole body duplication during mi... 11 15 23.8% 0.00959 50653
[View Peptides]   SPC72 Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them... 8 8 16.1% 0.00356 72105
[View Peptides]   ENO2 Enolase II, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosp... 4 4 14.2% 0.00253 46914
[View Peptides]   FBA1 Fructose 1,6-bisphosphate aldolase, required for glycolysis and gluconeogenesis; catalyzes conversio... 2 3 13.1% 0.00231 39621
[View Peptides]   RSM28 Mitochondrial ribosomal protein of the small subunit; genetic interactions suggest a possible role i... 2 2 12.7% 0.00153 41216
[View Peptides]   CDC19 Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvat... 6 6 12.6% 0.00332 54545
[View Peptides]   SFI1 Centrin (Cdc31p)-binding protein required for spindle pole body (SPB) duplication, localizes to the ... 9 10 9.3% 0.00293 112978
[View Peptides]   PMA1 Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plas... 7 8 8.6% 0.00241 99619
[View Peptides]   GYP1 Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, ... 2 2 8.5% 8.7E-4 73289
[View Peptides]   PMA2 Plasma membrane H+-ATPase, isoform of Pma1p, involved in pumping protons out of the cell; regulator ... 7 8 8.3% 0.00234 102172
[View Peptides]   PIR3 O-glycosylated covalently-bound cell wall protein required for cell wall stability; expression is ce... 2 3 8.3% 0.00256 33004
[View Peptides]   CIS3 Mannose-containing glycoprotein constituent of the cell wall; member of the PIR (proteins with inter... 2 3 7.9% 0.00366 23242
[View Peptides]   NOP1 Nucleolar protein, component of the small subunit processome complex, which is required for processi... 4 4 7.6% 0.00339 34465
[View Peptides]   NOP58 Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the ... 3 3 7.0% 0.00163 56956
[View Peptides]   MPP10 Component of the SSU processome and 90S preribosome, required for pre-18S rRNA processing, interacts... 2 2 6.4% 9.34E-4 66953
[View Peptides]   DED81 Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attach... 2 4 5.6% 0.002 62207
[View Peptides]   PIR1 O-glycosylated protein required for cell wall stability; attached to the cell wall via beta-1,3-gluc... 2 3 5.3% 0.00244 34622
[View Peptides]   HSP150 O-mannosylated heat shock protein that is secreted and covalently attached to the cell wall via beta... 2 3 4.7% 0.00215 38619
[View Peptides]   MLP1 Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... 5 5 4.2% 7.39E-4 218454
[View Peptides]   HSL7 Protein arginine N-methyltransferase that exhibits septin and Hsl1p-dependent bud neck localization ... 3 4 3.3% 0.00134 95153
[View Peptides]   MYO1 Type II myosin heavy chain, required for wild-type cytokinesis and cell separation; localizes to the... 2 2 2.7% 2.87E-4 223634
[View Peptides]   CHD1 Nucleosome remodeling factor that functions in regulation of transcription elongation; contains a ch... 2 2 2.5% 3.77E-4 168240

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