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Bait Protein: MLP2
Organism: Saccharomyces cerevisiae
Publication: Keck JM, Jones MH, Wong CC, Binkley J, Chen D, Jaspersen SL, Holinger EP, Xu T, Niepel M, Rout MP, Vogel J, Sidow A, Yates JR, Winey M., A Cell Cycle Phosphoproteome of the Yeast Centrosome, Science 24 June 2011: 1557-1561.
Comments: #24 Asynchronous Prep3-TiO2 Phosphopeptide enrichment

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FILTERED Run Results: [unfilter]

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  Hit Protein Protein Desc Sequence Count Spectrum Count Sequence Coverage NSAF Mol. Wt.
[View Peptides]   YIL002W-A Putative protein of unknown function; identified by expression profiling and mass spectrometry 11 41 98.6% 0.126 7729
[View Peptides]   RPP1B Ribosomal protein P1 beta, component of the ribosomal stalk, which is involved in interaction of tra... 2 6 67.0% 0.012 10668
[View Peptides]   CMD1 Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, act... 4 11 66.0% 0.0159 16135
[View Peptides]   CDC31 Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication i... 9 11 62.1% 0.0145 18751
[View Peptides]   SPC42 Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate att... 39 107 57.6% 0.0626 42271
[View Peptides]   SPC29 Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inn... 26 72 56.9% 0.0604 29280
[View Peptides]   MLP2 Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... 80 252 49.9% 0.0319 195140
[View Peptides]   FBA1 Fructose 1,6-bisphosphate aldolase, required for glycolysis and gluconeogenesis; catalyzes conversio... 7 22 46.8% 0.013 39621
[View Peptides]   HTA1 Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of tw... 2 3 44.7% 0.00483 13989
[View Peptides]   HTA2 Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of tw... 2 3 44.7% 0.00483 13989
[View Peptides]   BFA1 Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pa... 15 29 40.1% 0.0107 66087
[View Peptides]   SPC110 Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nu... 36 79 38.3% 0.0178 111782
[View Peptides]   CNM67 Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclea... 15 34 34.9% 0.0124 67400
[View Peptides]   YLR146W-A Putative protein of unknown function 2 2 33.9% 0.00685 7078
[View Peptides]   CDC19 Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvat... 10 22 31.6% 0.00934 54545
[View Peptides]   ASC1 G-protein beta subunit and guanine nucleotide dissociation inhibitor for Gpa2p; ortholog of RACK1 th... 6 11 29.5% 0.00732 34805
[View Peptides]   YDJ1 Protein chaperone involved in regulation of the HSP90 and HSP70 functions; involved in protein trans... 7 20 28.6% 0.0104 44671
[View Peptides]   ENO2 Enolase II, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosp... 11 40 25.2% 0.0194 46914
[View Peptides]   NUD1 Component of the spindle pole body outer plaque, required for exit from mitosis 25 81 24.4% 0.0202 94104
[View Peptides]   RPP2A Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction... 2 3 23.6% 0.00601 10746
[View Peptides]   PSA1 GDP-mannose pyrophosphorylase (mannose-1-phosphate guanyltransferase), synthesizes GDP-mannose from ... 5 12 19.9% 0.00706 39566
[View Peptides]   ENO1 Enolase I, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosph... 8 31 19.7% 0.0151 46816
[View Peptides]   PMA1 Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plas... 10 15 17.4% 0.00347 99619
[View Peptides]   BBP1 Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periph... 5 5 15.1% 0.00276 45384
[View Peptides]   YOR051C Nuclear protein that inhibits replication of Brome mosaic virus in S. cerevisiae, which is a model s... 2 2 13.8% 0.00103 47352
[View Peptides]   CWP1 Cell wall mannoprotein that localizes specifically to birth scars of daughter cells, linked to a bet... 2 7 13.4% 0.00622 24268
[View Peptides]   KAR1 Essential protein involved in karyogamy during mating and in spindle pole body duplication during mi... 4 8 12.7% 0.00392 50653
[View Peptides]   BAG7 Rho GTPase activating protein (RhoGAP), stimulates the intrinsic GTPase activity of Rho1p, which pla... 2 2 12.0% 0.00104 46216
[View Peptides]   PDC1 Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates... 3 7 11.5% 0.00264 61495
[View Peptides]   NSR1 Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ri... 2 2 11.4% 0.00103 44535
[View Peptides]   YEF3 Translational elongation factor 3, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes b... 5 13 11.2% 0.00264 115945
[View Peptides]   RHR2 Constitutively expressed isoform of DL-glycerol-3-phosphatase; involved in glycerol biosynthesis, in... 2 4 10.0% 0.00313 30439
[View Peptides]   ARF1 ADP-ribosylation factor, GTPase of the Ras superfamily involved in regulation of coated vesicle form... 2 7 9.9% 0.00821 20529
[View Peptides]   MPS2 Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required f... 3 18 9.8% 0.00988 44585
[View Peptides]   HSP82 Hsp90 chaperone required for pheromone signaling and negative regulation of Hsf1p; docks with Tom70p... 5 20 9.6% 0.00599 81406
[View Peptides]   HSC82 Cytoplasmic chaperone of the Hsp90 family, redundant in function and nearly identical with Hsp82p, a... 5 20 9.6% 0.00602 80900
[View Peptides]   TEF1, TEF2 Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction ... 3 5 9.6% 0.00232 50033
[View Peptides]   SPC72 Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them... 4 7 9.3% 0.00239 72105
[View Peptides]   GFA1 Glutamine-fructose-6-phosphate amidotransferase, catalyzes the formation of glucosamine-6-P and glut... 4 6 9.2% 0.00178 80047
[View Peptides]   KRI1 Essential nucleolar protein required for 40S ribosome biogenesis; physically and functionally intera... 2 2 9.1% 7.19E-4 68654
[View Peptides]   GUS1 Glutamyl-tRNA synthetase (GluRS), forms a complex with methionyl-tRNA synthetase (Mes1p) and Arc1p; ... 3 3 8.5% 9.0E-4 80843
[View Peptides]   NOP2 Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and la... 2 9 8.4% 0.00309 69812
[View Peptides]   SLX5 Subunit of the Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex, stimulated by SUMO-modified... 2 2 8.1% 6.86E-4 71071
[View Peptides]   UBP9 Ubiquitin carboxyl-terminal hydrolase, ubiquitin-specific protease that cleaves ubiquitin-protein fu... 2 2 7.8% 5.63E-4 86244
[View Peptides]   NIP1 eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of ... 2 3 7.5% 7.85E-4 93204
[View Peptides]   YRA1 RNA binding protein required for export of poly(A)+ mRNA from the nucleus; proposed to couple mRNA e... 2 2 7.1% 0.00188 24956
[View Peptides]   STU2 Microtubule-associated protein (MAP) of the XMAP215/Dis1 family; regulates microtubule dynamics duri... 2 2 6.9% 4.78E-4 100918
[View Peptides]   HSP42 Small heat shock protein (sHSP) with chaperone activity; forms barrel-shaped oligomers that suppress... 2 3 6.7% 0.0017 42817
[View Peptides]   TUB3 Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form... 2 3 6.3% 0.00143 49694
[View Peptides]   TUB1 Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form... 2 3 6.3% 0.00143 49800
[View Peptides]   EFT2, EFT1 Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein s... 3 10 6.1% 0.00252 93289
[View Peptides]   CYS3 Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathw... 2 9 6.1% 0.00485 42542
[View Peptides]   DBP9 ATP-dependent RNA helicase of the DEAD-box family involved in biogenesis of the 60S ribosomal subuni... 2 3 5.7% 0.00107 68060
[View Peptides]   DED81 Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attach... 2 11 5.6% 0.00422 62207
[View Peptides]   RPN3 Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of th... 2 8 5.5% 0.00325 60393
[View Peptides]   CDC14 Protein phosphatase required for mitotic exit; located in the nucleolus until liberated by the FEAR ... 6 18 5.3% 0.00694 61907
[View Peptides]   PGK1 3-phosphoglycerate kinase, catalyzes transfer of high-energy phosphoryl groups from the acyl phospha... 2 2 5.3% 0.00102 44738
[View Peptides]   ARB1 ATPase of the ATP-binding cassette (ABC) family involved in 40S and 60S ribosome biogenesis, has sim... 2 2 5.2% 6.96E-4 68377
[View Peptides]   ADE5,7 Bifunctional enzyme of the 'de novo' purine nucleotide biosynthetic pathway, contains aminoimidazole... 2 2 5.1% 5.3E-4 86068
[View Peptides]   ASK10 Component of the RNA polymerase II holoenzyme, phosphorylated in response to oxidative stress; has a... 2 2 4.5% 3.71E-4 126864
[View Peptides]   ARO2 Bifunctional chorismate synthase and flavin reductase, catalyzes the conversion of 5-enolpyruvylshik... 2 3 4.3% 0.00169 40838
[View Peptides]   SFI1 Centrin (Cdc31p)-binding protein required for spindle pole body (SPB) duplication, localizes to the ... 2 3 3.5% 6.73E-4 112978
[View Peptides]   YDR261C-D Retrotransposon TYA Gag and TYB Pol genes; in YDRCTY1-3 TYB is mutant and probably non-functional 3 8 3.4% 0.00106 181660
[View Peptides]   DNA2 Tripartite DNA replication factor with single-stranded DNA-dependent ATPase, ATP-dependent nuclease,... 2 2 3.3% 2.79E-4 171694
[View Peptides]   YLR157C-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 3 8 3.1% 9.68E-4 198544
[View Peptides]   YGR027W-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 3 8 3.1% 9.68E-4 198656
[View Peptides]   YJR029W Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 3 8 3.1% 9.68E-4 198616
[View Peptides]   YLR227W-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 3 8 3.1% 9.68E-4 198404
[View Peptides]   YBL005W-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 3 8 3.1% 9.68E-4 198968
[View Peptides]   YGR161C-D Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 3 8 3.1% 9.68E-4 198558
[View Peptides]   YDR365W-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 3 8 3.1% 9.68E-4 198658
[View Peptides]   YGR109W-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 2 2 2.8% 2.75E-4 178419
[View Peptides]   PFK1 Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaer... 2 5 2.8% 0.00108 107970
[View Peptides]   MLP1 Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... 2 2 2.7% 2.27E-4 218454
[View Peptides]   VMA2 Subunit B of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), a... 2 5 2.7% 0.00205 57749
[View Peptides]   ACC1 Acetyl-CoA carboxylase, biotin containing enzyme that catalyzes the carboxylation of acetyl-CoA to f... 2 2 1.9% 1.9E-4 250351
[View Peptides]   FAS1 Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty a... 2 2 1.8% 2.07E-4 228689
[View Peptides]   ESC1 Protein localized to the nuclear periphery, involved in telomeric silencing; interacts with PAD4-dom... 2 2 1.7% 2.56E-4 187137

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