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Bait Protein: MLP2
Organism: Saccharomyces cerevisiae
Publication: Keck JM, Jones MH, Wong CC, Binkley J, Chen D, Jaspersen SL, Holinger EP, Xu T, Niepel M, Rout MP, Vogel J, Sidow A, Yates JR, Winey M., A Cell Cycle Phosphoproteome of the Yeast Centrosome, Science 24 June 2011: 1557-1561.
Comments: #25 Asynchronous Prep3-TiO2 Flowthrough

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  Hit Protein Protein Desc Sequence Count Spectrum Count Sequence Coverage NSAF Mol. Wt.
[View Peptides]   SNU13 RNA binding protein, part of U3 snoRNP involved in rRNA processing, part of U4/U6-U5 tri-snRNP invol... 3 7 49.2% 0.0127 13569
[View Peptides]   PDC1 Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates... 18 93 44.4% 0.0379 61495
[View Peptides]   HHT1, HHT2 Histone H3, core histone protein required for chromatin assembly, part of heterochromatin-mediated t... 4 12 37.5% 0.0202 15356
[View Peptides]   ENO2 Enolase II, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosp... 14 40 35.0% 0.021 46914
[View Peptides]   CMD1 Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, act... 2 22 34.0% 0.0343 16135
[View Peptides]   CDC19 Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvat... 13 55 33.8% 0.0252 54545
[View Peptides]   HTB1 Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly id... 3 4 32.1% 0.00701 14252
[View Peptides]   HTB2 Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly id... 3 4 32.1% 0.00701 14237
[View Peptides]   HTA1 Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of tw... 3 10 28.8% 0.0174 13989
[View Peptides]   HTA2 Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of tw... 3 10 28.8% 0.0174 13989
[View Peptides]   ASC1 G-protein beta subunit and guanine nucleotide dissociation inhibitor for Gpa2p; ortholog of RACK1 th... 5 10 26.0% 0.00719 34805
[View Peptides]   MLP2 Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... 40 103 24.9% 0.0141 195140
[View Peptides]   EFT2, EFT1 Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein s... 11 16 23.5% 0.00436 93289
[View Peptides]   ADH1 Alcohol dehydrogenase, fermentative isozyme active as homo- or heterotetramers; required for the red... 5 13 21.0% 0.00857 36849
[View Peptides]   FBA1 Fructose 1,6-bisphosphate aldolase, required for glycolysis and gluconeogenesis; catalyzes conversio... 5 14 20.3% 0.00895 39621
[View Peptides]   TEF1, TEF2 Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction ... 12 35 20.1% 0.0175 50033
[View Peptides]   HHF1, HHF2 Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two... 2 4 19.4% 0.00891 11368
[View Peptides]   YDJ1 Protein chaperone involved in regulation of the HSP90 and HSP70 functions; involved in protein trans... 2 4 19.3% 0.00224 44671
[View Peptides]   PGK1 3-phosphoglycerate kinase, catalyzes transfer of high-energy phosphoryl groups from the acyl phospha... 6 11 19.0% 0.00607 44738
[View Peptides]   BMH1 14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and D... 2 3 18.4% 0.00258 30091
[View Peptides]   SPC29 Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inn... 4 14 18.2% 0.0127 29280
[View Peptides]   BMH2 14-3-3 protein, minor isoform; controls proteome at post-transcriptional level, binds proteins and D... 2 3 17.9% 0.00252 31061
[View Peptides]   TPI1 Triose phosphate isomerase, abundant glycolytic enzyme; mRNA half-life is regulated by iron availabi... 2 3 17.7% 0.00278 26795
[View Peptides]   SPC42 Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate att... 4 21 17.4% 0.0133 42271
[View Peptides]   RPP0 Conserved ribosomal protein P0 similar to rat P0, human P0, and E. coli L10e; shown to be phosphoryl... 3 5 16.3% 0.00368 33717
[View Peptides]   CNM67 Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclea... 11 40 16.2% 0.0158 67400
[View Peptides]   PGI1 Glycolytic enzyme phosphoglucose isomerase, catalyzes the interconversion of glucose-6-phosphate and... 2 4 15.7% 0.00166 61299
[View Peptides]   CDC14 Protein phosphatase required for mitotic exit; located in the nucleolus until liberated by the FEAR ... 5 12 15.6% 0.005 61907
[View Peptides]   HOM6 Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third... 2 2 15.6% 0.00128 38502
[View Peptides]   CDC31 Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication i... 2 6 14.3% 0.00855 18751
[View Peptides]   TSA1 Thioredoxin peroxidase, acts as both a ribosome-associated and free cytoplasmic antioxidant; self-as... 2 3 13.8% 0.00351 21590
[View Peptides]   SPC110 Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nu... 10 22 12.5% 0.00535 111782
[View Peptides]   ACT1 Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functio... 2 3 12.3% 0.00184 41690
[View Peptides]   PMA1 Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plas... 8 13 12.0% 0.00325 99619
[View Peptides]   MET17 Methionine and cysteine synthase (O-acetyl homoserine-O-acetyl serine sulfhydrylase), required for s... 3 6 11.7% 0.0031 48672
[View Peptides]   GPM1 Tetrameric phosphoglycerate mutase, mediates the conversion of 3-phosphoglycerate to 2-phosphoglycer... 2 3 11.7% 0.00279 27609
[View Peptides]   SPC98 Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the... 4 6 10.6% 0.00163 98227
[View Peptides]   RNR2 Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiti... 2 3 10.0% 0.00173 46147
[View Peptides]   YDR261C-D Retrotransposon TYA Gag and TYB Pol genes; in YDRCTY1-3 TYB is mutant and probably non-functional 10 20 9.4% 0.00286 181660
[View Peptides]   RFC1 Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase t... 2 2 9.1% 5.33E-4 94903
[View Peptides]   NUD1 Component of the spindle pole body outer plaque, required for exit from mitosis 4 13 8.9% 0.00351 94104
[View Peptides]   YDR365W-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 10 20 8.5% 0.00261 198658
[View Peptides]   YDR098C-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 10 20 8.5% 0.00261 198594
[View Peptides]   YDR210C-D Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 10 20 8.5% 0.00261 198838
[View Peptides]   YDR316W-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 10 20 8.5% 0.00261 198526
[View Peptides]   YER138C Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 10 20 8.5% 0.00261 198561
[View Peptides]   NUP60 Subunit of the nuclear pore complex (NPC), functions to anchor Nup2p to the NPC in a process control... 2 2 8.5% 8.51E-4 59039
[View Peptides]   YHB1 Nitric oxide oxidoreductase, flavohemoglobin involved in nitric oxide detoxification; plays a role i... 3 8 8.5% 0.0046 44646
[View Peptides]   YEF3 Translational elongation factor 3, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes b... 6 14 8.2% 0.00308 115945
[View Peptides]   VMA2 Subunit B of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), a... 2 3 7.2% 0.00133 57749
[View Peptides]   URA7 Major CTP synthase isozyme (see also URA8), catalyzes the ATP-dependent transfer of the amide nitrog... 2 2 6.6% 7.93E-4 64710
[View Peptides]   MRM1 Ribose methyltransferase that modifies a functionally critical, conserved nucleotide in mitochondria... 2 5 6.3% 0.00278 46387
[View Peptides]   ATP2 Beta subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily c... 2 7 5.7% 0.00314 54794
[View Peptides]   DED81 Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attach... 2 2 5.4% 8.28E-4 62207
[View Peptides]   YOR051C Nuclear protein that inhibits replication of Brome mosaic virus in S. cerevisiae, which is a model s... 2 2 5.1% 0.00111 47352
[View Peptides]   GFA1 Glutamine-fructose-6-phosphate amidotransferase, catalyzes the formation of glucosamine-6-P and glut... 2 2 4.7% 6.4E-4 80047
[View Peptides]   ERG11 Lanosterol 14-alpha-demethylase, catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimeth... 2 2 4.7% 8.66E-4 60720
[View Peptides]   SSE1 ATPase that is a component of the heat shock protein Hsp90 chaperone complex; binds unfolded protein... 2 2 4.6% 6.62E-4 77367
[View Peptides]   SPC72 Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them... 2 2 4.0% 7.38E-4 72105
[View Peptides]   MES1 Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which in... 2 3 3.9% 9.17E-4 85678
[View Peptides]   DED1 ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all ye... 2 3 3.8% 0.00114 65553
[View Peptides]   MET6 Cobalamin-independent methionine synthase, involved in methionine biosynthesis and regeneration; req... 2 3 3.5% 8.97E-4 85860
[View Peptides]   ADE6 Formylglycinamidine-ribonucleotide (FGAM)-synthetase, catalyzes a step in the 'de novo' purine nucle... 2 2 3.4% 3.38E-4 148904
[View Peptides]   PYC2 Pyruvate carboxylase isoform, cytoplasmic enzyme that converts pyruvate to oxaloacetate; highly simi... 2 5 3.2% 9.72E-4 130167
[View Peptides]   CDC60 Cytosolic leucyl tRNA synthetase, ligates leucine to the appropriate tRNA 2 2 2.8% 4.21E-4 124141
[View Peptides]   TIF4631 Translation initiation factor eIF4G, subunit of the mRNA cap-binding protein complex (eIF4F) that al... 2 2 2.8% 4.82E-4 107101
[View Peptides]   URA2 Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes t... 3 4 2.5% 4.15E-4 245123
[View Peptides]   GCN1 Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn20p; proposed to stimulate ... 2 2 1.6% 1.72E-4 296697
[View Peptides]   UBR1 E3 ubiquitin ligase (N-recognin), forms heterodimer with Rad6p to ubiquitinate substrates in the N-e... 2 2 1.6% 2.35E-4 224836
[View Peptides]   ACC1 Acetyl-CoA carboxylase, biotin containing enzyme that catalyzes the carboxylation of acetyl-CoA to f... 2 3 1.5% 3.08E-4 250351
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