Bait Protein: | MLP2 |
Organism: | Saccharomyces cerevisiae |
Publication: | Keck JM, Jones MH, Wong CC, Binkley J, Chen D, Jaspersen SL, Holinger EP, Xu T, Niepel M, Rout MP, Vogel J, Sidow A, Yates JR, Winey M., A Cell Cycle Phosphoproteome of the Yeast Centrosome, Science 24 June 2011: 1557-1561. |
Comments: | #01 Alpha factor Prep1-TiO2 Phosphopeptide enrichment |
Raw Data: |
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UNFILTERED Run Results:
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Hit Protein | Protein Desc | Sequence Count | Spectrum Count | Sequence Coverage | NSAF | Mol. Wt. | |
[ |
YIL002W-A | Putative protein of unknown function; identified by expression profiling and mass spectrometry | 16 | 20 | 92.8% | 0.238 | 7729 |
[ |
MLP2 | Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... | 187 | 237 | 66.8% | 0.116 | 195140 |
[ |
CDC31 | Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication i... | 13 | 15 | 61.5% | 0.0765 | 18751 |
[ |
SPC42 | Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate att... | 29 | 32 | 61.4% | 0.0724 | 42271 |
[ |
SPC110 | Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nu... | 72 | 81 | 51.4% | 0.0705 | 111782 |
[ |
CNM67 | Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclea... | 24 | 24 | 48.2% | 0.0339 | 67400 |
[ |
SPC29 | Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inn... | 13 | 15 | 47.4% | 0.0487 | 29280 |
[ |
CMD1 | Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, act... | 4 | 4 | 44.9% | 0.0223 | 16135 |
[ |
TUB4 | Gamma-tubulin, involved in nucleating microtubules from both the cytoplasmic and nuclear faces of th... | 9 | 9 | 39.5% | 0.0156 | 52628 |
[ |
BBP1 | Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periph... | 10 | 10 | 31.2% | 0.0213 | 45384 |
[ |
RPL5 | Protein component of the large (60S) ribosomal subunit with similarity to E. coli L18 and rat L5 rib... | 9 | 11 | 29.0% | 0.0304 | 33715 |
[ |
Reverse_YLR457C | NBP1 SGDID:S000004449, Chr XII from 1056768-1055809, reverse complement, Verified ORF, "Component of... | 4 | 4 | 24.5% | 0.0103 | 37354 |
[ |
TCP1 | Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; inv... | 5 | 5 | 23.8% | 0.00735 | 60481 |
[ |
NBP1 | Spindle pole body (SPB) component, required for the insertion of the duplication plaque into the nuc... | 6 | 6 | 21.6% | 0.0154 | 37354 |
[ |
STM1 | Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with r... | 4 | 4 | 21.2% | 0.012 | 29995 |
[ |
SPC97 | Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the... | 10 | 22 | 20.2% | 0.022 | 96825 |
[ |
Reverse_YML063W,... | RPS1B SGDID:S000004528, Chr XIII from 146482-147249, Verified ORF, "Ribosomal protein 10 (rp10) of t... | 2 | 2 | 19.6% | 0.00642 | 28812 |
[ |
BFA1 | Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pa... | 7 | 7 | 18.5% | 0.01 | 66087 |
[ |
RPP2A | Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction... | 1 | 1 | 17.0% | 0.00775 | 10746 |
[ |
Reverse_YPL124W | SPC29 SGDID:S000006045, Chr XVI from 316754-317515, Verified ORF, "Inner plaque spindle pole body (S... | 1 | 1 | 16.6% | 0.00323 | 29280 |
[ |
KAR1 | Essential protein involved in karyogamy during mating and in spindle pole body duplication during mi... | 5 | 5 | 16.4% | 0.00948 | 50653 |
[ |
Reverse_YKL042W | SPC42 SGDID:S000001525, Chr XI from 358119-359210, Verified ORF, "Central plaque component of spindl... | 4 | 4 | 16.0% | 0.00903 | 42271 |
[ |
Reverse_YJR053W | BFA1 SGDID:S000003814, Chr X from 533941-535665, Verified ORF, "Component of the GTPase-activating B... | 3 | 3 | 14.1% | 0.00429 | 66087 |
[ |
MPP10 | Component of the SSU processome and 90S preribosome, required for pre-18S rRNA processing, interacts... | 4 | 4 | 13.7% | 0.00554 | 66953 |
[ |
MPS2 | Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required f... | 5 | 5 | 13.4% | 0.0106 | 44585 |
[ |
SSB2 | Cytoplasmic ATPase that is a ribosome-associated molecular chaperone, functions with J-protein partn... | 4 | 4 | 12.9% | 0.00536 | 66595 |
[ |
SSB1 | Cytoplasmic ATPase that is a ribosome-associated molecular chaperone, functions with J-protein partn... | 4 | 4 | 12.9% | 0.00536 | 66602 |
[ |
NUD1 | Component of the spindle pole body outer plaque, required for exit from mitosis | 8 | 8 | 12.6% | 0.00772 | 94104 |
[ |
SPC98 | Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the... | 5 | 11 | 12.3% | 0.0107 | 98227 |
[ |
SSA1 | ATPase involved in protein folding and nuclear localization signal (NLS)-directed nuclear transport;... | 7 | 7 | 12.1% | 0.00896 | 69768 |
[ |
PMA1 | Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plas... | 8 | 9 | 10.5% | 0.00805 | 99619 |
[ |
HSL7 | Protein arginine N-methyltransferase that exhibits septin and Hsl1p-dependent bud neck localization ... | 8 | 8 | 10.5% | 0.00794 | 95153 |
[ |
EFT2, EFT1 | Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein s... | 4 | 4 | 9.3% | 0.0039 | 93289 |
[ |
KAR2 | ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding i... | 4 | 4 | 8.7% | 0.00482 | 74468 |
[ |
Reverse_YPL131W,... | RPL5 SGDID:S000006052, Chr XVI from 303120-304013, Verified ORF, "Protein component of the large (60... | 1 | 1 | 7.7% | 0.00276 | 33715 |
[ |
Reverse_YLR150W | STM1 SGDID:S000004140, Chr XII from 440468-441289, Verified ORF, "Protein that binds G4 quadruplex a... | 1 | 1 | 7.7% | 0.003 | 29995 |
[ |
Reverse_YIL149C | MLP2 SGDID:S000001411, Chr IX from 68067-63028, reverse complement, Verified ORF, "Myosin-like prote... | 6 | 6 | 6.6% | 0.00293 | 195140 |
[ |
Reverse_YMR142C | RPL13B SGDID:S000004750, Chr XIII from 551206-551203,550800-550205, reverse complement, Verified ORF... | 1 | 1 | 6.5% | 0.00411 | 22525 |
[ |
MLP1 | Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... | 8 | 8 | 6.2% | 0.0035 | 218454 |
[ |
Reverse_YGL173C | KEM1 SGDID:S000003141, Chr VII from 180119-175533, reverse complement, Verified ORF, "5'-3' exonucle... | 4 | 4 | 5.1% | 0.00215 | 175459 |
[ |
Reverse_YDR172W | SUP35 SGDID:S000002579, Chr IV from 808319-810376, Verified ORF, "Translation termination factor eRF... | 2 | 2 | 5.0% | 0.00239 | 76551 |
[ |
Reverse_YGR254W | ENO1 SGDID:S000003486, Chr VII from 1000932-1002245, Verified ORF, "Enolase I, a phosphopyruvate hyd... | 1 | 1 | 5.0% | 0.00188 | 46816 |
[ |
Reverse_YMR186W | HSC82 SGDID:S000004798, Chr XIII from 632354-634471, Verified ORF, "Cytoplasmic chaperone of the Hsp... | 3 | 3 | 4.5% | 0.00349 | 80900 |
[ |
Reverse_YLR044C | PDC1 SGDID:S000004034, Chr XII from 234082-232391, reverse complement, Verified ORF, "Major of three... | 1 | 1 | 4.4% | 0.00146 | 61495 |
[ |
Reverse_YNL188W | KAR1 SGDID:S000005132, Chr XIV from 286309-287610, Verified ORF, "Essential protein involved in kary... | 1 | 1 | 4.4% | 0.00189 | 50653 |
[ |
Reverse_YPL255W | BBP1 SGDID:S000006176, Chr XVI from 67725-68882, Verified ORF, "Protein required for the spindle pol... | 1 | 1 | 4.4% | 0.00213 | 45384 |
[ |
Reverse_YAL047C | SPC72 SGDID:S000000045, Chr I from 56858-54990, reverse complement, Verified ORF, "Component of the ... | 2 | 2 | 4.3% | 0.00264 | 72105 |
[ |
RPL13B | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl13Ap; not essential f... | 1 | 1 | 4.0% | 0.00413 | 22525 |
[ |
KEM1 | Evolutionarily-conserved 5'-3' exonuclease component of cytoplasmic processing (P) bodies involved i... | 2 | 2 | 3.9% | 0.00108 | 175459 |
[ |
Reverse_YNL126W | SPC98 SGDID:S000005070, Chr XIV from 387229-389769, Verified ORF, "Component of the microtubule-nucl... | 1 | 1 | 3.8% | 9.7E-4 | 98227 |
[ |
Reverse_YDR356W | SPC110 SGDID:S000002764, Chr IV from 1186098-1188932, Verified ORF, "Inner plaque spindle pole body ... | 2 | 2 | 3.7% | 0.00174 | 111782 |
[ |
SPB1 | AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation;... | 1 | 1 | 3.7% | 9.77E-4 | 96485 |
[ |
SPC72 | Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them... | 2 | 2 | 3.7% | 0.00264 | 72105 |
[ |
Reverse_YCL054W | SPB1 SGDID:S000000559, Chr III from 31449-33974, Verified ORF, "AdoMet-dependent methyltransferase i... | 2 | 2 | 3.4% | 0.00195 | 96485 |
[ |
Reverse_YJL034W | KAR2 SGDID:S000003571, Chr X from 381243-383291, Verified ORF, "ATPase involved in protein import in... | 1 | 1 | 2.9% | 0.0012 | 74468 |
[ |
POM152 | Nuclear pore membrane glycoprotein; may be involved in duplication of nuclear pores and nuclear pore... | 2 | 2 | 2.7% | 0.00123 | 151652 |
[ |
Reverse_YJR002W | MPP10 SGDID:S000003762, Chr X from 438779-440560, Verified ORF, "Component of the SSU processome, wh... | 1 | 1 | 2.5% | 0.00138 | 66953 |
[ |
Reverse_YGL008C | PMA1 SGDID:S000002976, Chr VII from 482671-479915, reverse complement, Verified ORF, "Plasma membran... | 2 | 2 | 2.3% | 0.00179 | 99619 |
[ |
Reverse_YMR129W | POM152 SGDID:S000004736, Chr XIII from 527803-531816, Verified ORF, "Nuclear pore membrane glycoprot... | 1 | 1 | 2.2% | 6.14E-4 | 151652 |
[ |
Reverse_YKR095W | MLP1 SGDID:S000001803, Chr XI from 619447-625074, Verified ORF, "Myosin-like protein associated with... | 2 | 2 | 1.9% | 8.76E-4 | 218455 |
[ |
Reverse_YHR172W | SPC97 SGDID:S000001215, Chr VIII from 448335-450806, Verified ORF, "Component of the microtubule-nuc... | 1 | 1 | 1.8% | 9.97E-4 | 96825 |
[ |
Reverse_YOR373W | NUD1 SGDID:S000005900, Chr XV from 1036830-1039385, Verified ORF, "Component of the spindle pole bod... | 1 | 1 | 1.5% | 9.64E-4 | 94104 |
[ |
Reverse_YNL014W | HEF3 SGDID:S000004959, Chr XIV from 606320-609454, Verified ORF, "Translational elongation factor EF... | 1 | 1 | 1.0% | 7.86E-4 | 115868 |
[ |
FKS1 | Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic sub... | 1 | 1 | 0.5% | 4.38E-4 | 214849 |