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Bait Protein: VAM3
Organism: Saccharomyces cerevisiae
Publication: Hao Xu , Youngsoo Jun, James Thompson, John Yates and William Wickner (2010) HOPS Prevents the Disassembly of Trans-SNARE Complexes by Sec17p/Sec18p during Membrane Fusion [In Press]
Comments: hx21: both fusion and trans-SNARE complex formation take place. Investigating Vam3p, Nyv1p and their partners in trans-SNARE complex

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UNFILTERED Run Results: [filter]

[View our MS interpretation guidelines.]
  Hit Protein Protein Desc Sequence Count Spectrum Count Sequence Coverage NSAF Mol. Wt.
[View Peptides]   gi|2119236, gi|4... sp|P35527|K1CI_HUMAN Keratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9), gi|545257|gb|AAC60... 60 367 79.9% 0.125 61987
[View Peptides]   KRT2 keratin 2 58 233 65.0% 0.0763 65865
[View Peptides]   Contaminant_KERA... 56 265 51.5% 0.087 65494
[View Peptides]   KRT10 keratin 10 49 450 59.5% 0.16 59519
[View Peptides]   SPA1_STAAU (P02976) Immunoglobulin G binding protein A precursor (IgG binding protein A) (Staphylococcal protei... 44 173 43.5% 0.0697 57320
[View Peptides]   contaminant_KERA... 29 75 49.3% 0.0282 59822
[View Peptides]   Contaminant_KERA... 28 72 51.0% 0.0323 51531
[View Peptides]   KRT5 keratin 5 25 73 38.3% 0.0261 62461
[View Peptides]   gi|34069 gi|34069|emb|CAA24760.1| unnamed protein product [Homo sapiens] 22 65 58.5% 0.0384 39219
[View Peptides]   contaminant_KERA... 21 43 46.1% 0.0194 50499
[View Peptides]   PHO8 Repressible alkaline phosphatase, a glycoprotein localized to the vacuole; regulated by levels of in... 16 43 37.6% 0.016 63004
[View Peptides]   KRT17 keratin 17 13 22 34.8% 0.0108 47974
[View Peptides]   VAM3 Syntaxin-related protein required for vacuolar assembly; functions with Vam7p in vacuolar protein tr... 12 33 56.9% 0.0246 32498
[View Peptides]   VAM7 Component of the vacuole SNARE complex involved in vacuolar morphogenesis; SNAP-25 homolog; function... 12 49 42.4% 0.0327 36711
[View Peptides]   KRT3 keratin 3 11 115 12.6% 0.0386 64511
[View Peptides]   YPK9 Vacuolar protein with a possible role in sequestering heavy metals; has similarity to the type V P-t... 11 14 10.7% 0.00201 166749
[View Peptides]   NYV1 v-SNARE component of the vacuolar SNARE complex involved in vesicle fusion; inhibits ATP-dependent C... 8 32 56.5% 0.0267 28964
[View Peptides]   contaminant_KERA... isb|A33652|A33652|probable keratin 16, type I, pseudogene - human 8 24 14.2% 0.0107 50915
[View Peptides]   KRT76 keratin 76 8 22 8.6% 0.00728 65871
[View Peptides]   FRA1 Protein involved in negative regulation of transcription of iron regulon; forms an iron independent ... 7 7 13.5% 0.00197 84924
[View Peptides]   VTI1 Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and ... 6 16 35.5% 0.0156 24668
[View Peptides]   YPF1 Putative protein of unknown function with similarity to a human minor histocompatibility antigen and... 6 9 15.2% 0.00324 67525
[View Peptides]   MNR2 Vacuolar membrane protein required for magnesium homeostasis; putative magnesium transporter; has si... 6 8 10.2% 0.00174 109736
[View Peptides]   contaminant_TRYP... null 6 17 8.3% 0.0157 23993
[View Peptides]   contaminant_NRL_... owl|| Immunoglobulin g1 (igg1) (mcg) with a hinge deletion, chain H... 5 9 12.9% 0.00444 46852
[View Peptides]   KRT19 keratin 19 5 9 9.5% 0.00475 44106
[View Peptides]   RPL2A, RPL2B Protein component of the large (60S) ribosomal subunit, identical to Rpl2Bp and has similarity to E.... 4 11 23.2% 0.00914 27408
[View Peptides]   gi|34033, gi|71526 pir||KRHU3 keratin 13, type I, cytoskeletal, long splice form - human, gi|34033|emb|CAA32786.1| ker... 4 8 9.4% 0.00369 49644
[View Peptides]   VNX1 Low affinity vacuolar membrane localized monovalent cation/H+ antiporter; member of the calcium exch... 4 5 7.4% 0.00116 102499
[View Peptides]   FPS1 Plasma membrane channel, member of major intrinsic protein (MIP) family; involved in efflux of glyce... 4 4 7.2% 0.00126 73877
[View Peptides]   UBB ubiquitin B 3 7 44.7% 0.0194 8565
[View Peptides]   RPS11A, RPS11B Protein component of the small (40S) ribosomal subunit; identical to Rps11Bp and has similarity to E... 3 3 23.1% 0.00406 17749
[View Peptides]   RPL17A Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl17Bp and has similari... 3 5 22.8% 0.00574 20549
[View Peptides]   RPL17B Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl17Ap and has similari... 3 5 22.8% 0.00574 20551
[View Peptides]   RPL28 Ribosomal protein of the large (60S) ribosomal subunit, has similarity to E. coli L15 and rat L27a r... 3 8 22.1% 0.0113 16722
[View Peptides]   MAM3 Protein required for normal mitochondrial morphology, has similarity to hemolysins 3 7 11.3% 0.00209 77712
[View Peptides]   ZRC1 Vacuolar membrane zinc transporter, transports zinc from the cytosol into the vacuole for storage; a... 3 5 9.7% 0.00239 48345
[View Peptides]   Contaminant_KERA... 3 9 3.9% 0.00356 57265
[View Peptides]   PHO91 Low-affinity phosphate transporter of the vacuolar membrane; deletion of pho84, pho87, pho89, pho90,... 3 4 3.8% 9.45E-4 99490
[View Peptides]   YCF1 Vacuolar glutathione S-conjugate transporter of the ATP-binding cassette family, has a role in detox... 3 3 2.5% 4.18E-4 171120
[View Peptides]   RPL20B Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl20Ap and has similari... 2 2 18.4% 0.00243 20713
[View Peptides]   RPL20A Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl20Bp and has similari... 2 2 18.0% 0.00237 21236
[View Peptides]   RPL31A Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl31Bp and has similari... 2 2 15.9% 0.00374 12953
[View Peptides]   RPL31B Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl31Ap and has similari... 2 2 15.9% 0.00374 12967
[View Peptides]   RPL19B, RPL19A Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl19Ap and has similari... 2 2 14.8% 0.00223 21704
[View Peptides]   RPL32 Protein component of the large (60S) ribosomal subunit, has similarity to rat L32 ribosomal protein;... 2 2 13.8% 0.00325 14771
[View Peptides]   RPL29 Protein component of the large (60S) ribosomal subunit, has similarity to rat L29 ribosomal protein;... 2 4 13.6% 0.0143 6669
[View Peptides]   RPS4B, RPS4A Protein component of the small (40S) ribosomal subunit; identical to Rps4Ap and has similarity to ra... 2 2 11.5% 0.00162 29410
[View Peptides]   YNL217W Hypothetical protein 2 3 8.6% 0.00194 37151
[View Peptides]   UGA1 Gamma-aminobutyrate (GABA) transaminase (4-aminobutyrate aminotransferase) involved in the 4-aminobu... 2 3 7.4% 0.00134 52946
[View Peptides]   AVT3 Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seve... 2 3 6.8% 9.15E-4 75459
[View Peptides]   ZRT3 Vacuolar membrane zinc transporter, transports zinc from storage in the vacuole to the cytoplasm whe... 2 4 5.6% 0.00168 55403
[View Peptides]   RPL3 Protein component of the large (60S) ribosomal subunit, has similarity to E. coli L3 and rat L3 ribo... 2 3 5.4% 0.00164 43758
[View Peptides]   INT-STD1, Contam... 1 2 6 4.6% 0.00209 69271
[View Peptides]   CPS1 Vacuolar carboxypeptidase yscS; expression is induced under low-nitrogen conditions 2 3 4.5% 0.0011 64597
[View Peptides]   CDC19 Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvat... 2 2 4.4% 8.45E-4 54545
[View Peptides]   YCK3 Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fus... 2 2 4.2% 8.06E-4 60261
[View Peptides]   PHO84 High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated... 2 6 3.4% 0.00216 64382
[View Peptides]   Reverse_YKR086W PRP16 SGDID:S000001794, Chr XI from 599499-602714, Verified ORF, "RNA helicase in the DEAH-box famil... 2 2 3.1% 3.94E-4 121652
[View Peptides]   YDR089W Protein of unknown function; deletion confers resistance to Nickel 2 2 3.1% 4.86E-4 98712
[View Peptides]   USP8 ubiquitin specific peptidase 8 2 2 2.2% 3.78E-4 127523
[View Peptides]   Reverse_YPL084W BRO1 SGDID:S000006005, Chr XVI from 394035-396569, Verified ORF, "Cytoplasmic class E vacuolar prote... 2 2 1.3% 5.0E-4 97276
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