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Bait Protein: ASF1
Organism: Saccharomyces cerevisiae
Publication: Green EM, Antczak AJ, Bailey AO, Franco AA, Wu KJ, Yates JR 3rd, Kaufman PD. Replication-independent histone deposition by the HIR complex and Asf1. Curr Biol. 2005 Nov 22;15(22):2044-9.
Comments: sample 3928 - Asf1-S-TEV-ZZ (in hir3delta mutant)

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UNFILTERED Run Results: [filter]

[View our MS interpretation guidelines.]
  Hit Protein Protein Desc Sequence Count Spectrum Count Sequence Coverage NSAF Mol. Wt.
[View Peptides]   gi|265412 gi|265412|gb|AAB25337.1| V8 protease [Staphylococcus aureus, Peptide, 276 aa] 104 1293 89.5% 0.719 29990
[View Peptides]   ASF1 Nucleosome assembly factor, involved in chromatin assembly and disassembly, anti-silencing protein t... 38 118 56.6% 0.0649 31603
[View Peptides]   EFB1 Translation elongation factor 1 beta; stimulates nucleotide exchange to regenerate EF-1 alpha-GTP fo... 9 11 39.3% 0.00819 22627
[View Peptides]   TDH3 Glyceraldehyde-3-phosphate dehydrogenase, isozyme 3, involved in glycolysis and gluconeogenesis; tet... 13 19 39.2% 0.00878 35747
[View Peptides]   TEF1, TEF2 Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction ... 20 47 37.3% 0.0157 50033
[View Peptides]   RPS14A Ribosomal protein 59 of the small subunit, required for ribosome assembly and 20S pre-rRNA processin... 6 7 37.2% 0.00784 14537
[View Peptides]   RPL31A Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl31Bp and has similari... 4 4 37.2% 0.00543 12953
[View Peptides]   RPL31B Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl31Ap and has similari... 4 4 37.2% 0.00543 12967
[View Peptides]   RPS14B Ribosomal protein 59 of the small subunit, required for ribosome assembly and 20S pre-rRNA processin... 6 7 37.0% 0.00778 14650
[View Peptides]   RAD53 Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autopho... 26 31 36.9% 0.00579 91962
[View Peptides]   RPS7A Protein component of the small (40S) ribosomal subunit, nearly identical to Rps7Bp; interacts with K... 6 6 34.7% 0.00485 21622
[View Peptides]   gi|2119236, gi|4... sp|P35527|K1CI_HUMAN Keratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9), gi|545257|gb|AAC60... 13 20 33.3% 0.00493 61987
[View Peptides]   RPP1A Ribosomal stalk protein P1 alpha, involved in the interaction between translational elongation facto... 2 5 32.1% 0.00724 10908
[View Peptides]   RPL4B Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl4Ap and has similarit... 7 8 31.8% 0.00339 39062
[View Peptides]   SSB1 Cytoplasmic ATPase that is a ribosome-associated molecular chaperone, functions with J-protein partn... 14 18 30.0% 0.00451 66602
[View Peptides]   CDC33 Cytoplasmic mRNA cap binding protein and translation initiation factor eIF4E; the eIF4E-cap complex ... 5 5 28.6% 0.0036 24254
[View Peptides]   MLC1 Essential light chain for Myo1p, light chain for Myo2p; stabilizes Myo2p by binding to the neck regi... 2 2 27.5% 0.00206 16444
[View Peptides]   RPL4A N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to ... 6 7 27.3% 0.00297 39092
[View Peptides]   contaminant_NRL_... owl|| Immunoglobulin g1 (igg1) (mcg) with a hinge deletion, chain H... 10 19 25.5% 0.00681 46852
[View Peptides]   RPL23B, RPL23A Protein component of the large (60S) ribosomal subunit, identical to Rpl23Ap and has similarity to E... 3 4 25.5% 0.00448 14473
[View Peptides]   RPS4B, RPS4A Protein component of the small (40S) ribosomal subunit; identical to Rps4Ap and has similarity to ra... 5 6 24.9% 0.00353 29410
[View Peptides]   SSB2 Cytoplasmic ATPase that is a ribosome-associated molecular chaperone, functions with J-protein partn... 12 16 24.5% 0.00401 66595
[View Peptides]   KRT10 keratin 10 12 17 24.1% 0.0044 59519
[View Peptides]   Contaminant_KERA... 15 19 23.5% 0.00453 65494
[View Peptides]   RPS15 Protein component of the small (40S) ribosomal subunit; has similarity to E. coli S19 and rat S15 ri... 2 3 22.5% 0.00324 16002
[View Peptides]   STM1 Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with r... 6 7 22.0% 0.00393 29995
[View Peptides]   RPS8B, RPS8A Protein component of the small (40S) ribosomal subunit; identical to Rps8Ap and has similarity to ra... 3 3 22.0% 0.0023 22490
[View Peptides]   DCD1 Deoxycytidine monophosphate (dCMP) deaminase required for dCTP and dTTP synthesis; expression is NOT... 2 2 20.5% 9.84E-4 35646
[View Peptides]   RPS7B Protein component of the small (40S) ribosomal subunit, nearly identical to Rps7Ap; interacts with K... 3 3 20.0% 0.00242 21634
[View Peptides]   RPS18B, RPS18A Protein component of the small (40S) ribosomal subunit; nearly identical to Rps18Ap and has similari... 3 3 19.9% 0.00315 17038
[View Peptides]   RPS19B Protein component of the small (40S) ribosomal subunit, required for assembly and maturation of pre-... 3 4 19.4% 0.00426 15891
[View Peptides]   RPS19A Protein component of the small (40S) ribosomal subunit, required for assembly and maturation of pre-... 3 4 19.4% 0.00426 15917
[View Peptides]   TDH2 Glyceraldehyde-3-phosphate dehydrogenase, isozyme 2, involved in glycolysis and gluconeogenesis; tet... 5 5 19.0% 0.00231 35847
[View Peptides]   RPL12B, RPL12A Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl12Ap; rpl12a rpl12b d... 3 3 18.2% 0.00279 17823
[View Peptides]   RPL10 Protein component of the large (60S) ribosomal subunit, responsible for joining the 40S and 60S subu... 2 3 18.1% 0.00208 25361
[View Peptides]   IBI2 Protein of unknown function that associates with ribosomes; green fluorescent protein (GFP)-fusion p... 2 2 17.8% 0.00156 22179
[View Peptides]   SRO9 Cytoplasmic RNA-binding protein that associates with translating ribosomes; involved in heme regulat... 4 4 17.1% 0.00141 48060
[View Peptides]   RPS13 Protein component of the small (40S) ribosomal subunit; has similarity to E. coli S15 and rat S13 ri... 2 2 16.6% 0.00203 17029
[View Peptides]   KRT2 keratin 2 6 6 16.6% 0.00143 65865
[View Peptides]   VMA2 Subunit B of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), a... 5 5 16.4% 0.00148 57749
[View Peptides]   RPL17B Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl17Ap and has similari... 2 2 15.8% 0.00167 20551
[View Peptides]   RPL17A Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl17Bp and has similari... 2 2 15.8% 0.00167 20549
[View Peptides]   contaminant_NRL_... owl|| Immunoglobulin g1 (igg1) (mcg) with a hinge deletion, chain L... 2 2 13.9% 0.00142 22815
[View Peptides]   RPL14B Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl14Ap and has similari... 2 2 13.8% 0.00222 15153
[View Peptides]   RPL14A N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to ... 2 2 13.8% 0.00222 15167
[View Peptides]   YDJ1 Protein chaperone involved in regulation of the HSP90 and HSP70 functions; involved in protein trans... 5 5 13.2% 0.00188 44671
[View Peptides]   RPS25B Protein component of the small (40S) ribosomal subunit; nearly identical to Rps25Ap and has similari... 2 4 13.0% 0.00568 12009
[View Peptides]   RPS25A Protein component of the small (40S) ribosomal subunit; nearly identical to Rps25Bp and has similari... 2 4 13.0% 0.00568 12039
[View Peptides]   ARC1 Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetases (Mes1p and Gus1p), delivering t... 3 3 12.8% 0.00122 42084
[View Peptides]   PMA1 Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plas... 5 6 12.1% 0.001 99619
[View Peptides]   YHR020W Protein of unknown function that may interact with ribosomes, based on co-purification experiments; ... 5 5 11.9% 0.00112 77386
[View Peptides]   gi|34069 gi|34069|emb|CAA24760.1| unnamed protein product [Homo sapiens] 3 4 10.4% 0.00172 39219
[View Peptides]   RPS2 Protein component of the small (40S) subunit, essential for control of translational accuracy; phosp... 3 3 10.2% 0.00181 27450
[View Peptides]   DED1 ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all ye... 3 4 10.1% 0.00102 65553
[View Peptides]   KRT5 keratin 5 4 5 9.8% 0.0013 62461
[View Peptides]   NPL3 RNA-binding protein that promotes elongation, regulates termination, and carries poly(A) mRNA from n... 2 3 9.4% 0.00111 45407
[View Peptides]   RPS24A, RPS24B Protein component of the small (40S) ribosomal subunit; identical to Rps24Bp and has similarity to r... 2 2 8.9% 0.00227 15329
[View Peptides]   SSZ1 Hsp70 protein that interacts with Zuo1p (a DnaJ homolog) to form a ribosome-associated complex that ... 3 3 8.7% 8.56E-4 58238
[View Peptides]   AZR1 Plasma membrane transporter of the major facilitator superfamily, involved in resistance to azole dr... 2 2 8.6% 5.01E-4 67169
[View Peptides]   NSR1 Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ri... 3 3 8.2% 0.00111 44535
[View Peptides]   RPS1B Ribosomal protein 10 (rp10) of the small (40S) subunit; nearly identical to Rps1Ap and has similarit... 2 2 8.2% 0.0012 28812
[View Peptides]   RPS1A Ribosomal protein 10 (rp10) of the small (40S) subunit; nearly identical to Rps1Bp and has similarit... 2 2 8.2% 0.0012 28743
[View Peptides]   SSA2 ATP binding protein involved in protein folding and vacuolar import of proteins; member of heat shoc... 2 2 8.0% 4.8E-4 69470
[View Peptides]   SSA1 ATPase involved in protein folding and nuclear localization signal (NLS)-directed nuclear transport;... 2 2 7.9% 4.78E-4 69768
[View Peptides]   RPS23B, RPS23A Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accurac... 2 2 7.6% 0.00212 16038
[View Peptides]   PMA2 Plasma membrane H+-ATPase, isoform of Pma1p, involved in pumping protons out of the cell; regulator ... 3 4 7.5% 6.48E-4 102172
[View Peptides]   SLX8 Subunit of the Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex; stimulated by prior attachm... 2 3 7.3% 0.00168 30764
[View Peptides]   BFR2 Essential protein that is a component of 90S preribosomes; may be involved in rRNA processing; multi... 2 2 7.1% 5.75E-4 61203
[View Peptides]   AVT4 Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seve... 2 2 6.6% 4.3E-4 80026
[View Peptides]   GFA1 Glutamine-fructose-6-phosphate amidotransferase, catalyzes the formation of glucosamine-6-P and glut... 2 2 6.4% 4.28E-4 80047
[View Peptides]   HIR2 Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene tra... 3 3 6.3% 5.26E-4 98445
[View Peptides]   DBP2 Essential ATP-dependent RNA helicase of the DEAD-box protein family, involved in nonsense-mediated m... 2 2 5.5% 5.62E-4 60999
[View Peptides]   contaminant_KERA... 2 2 4.8% 5.46E-4 59822
[View Peptides]   YEF3 Translational elongation factor 3, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes b... 2 2 3.0% 2.94E-4 115945
[View Peptides]   KRT3 keratin 3 2 5 2.1% 0.00122 64511
[View Peptides]   YLR001C Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purifi... 2 2 1.3% 3.56E-4 97618
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