D bir1

DTASelect v1.9
/data/2/shabadu/february2005/sandall/bir1
/scratch/yates/yrcdb/cerv4932.FASTAC
SEQUEST 2.7 in SQT format.

Jump to the summary table.

sequest.params modifications:

*	X	0.0
#	X	0.0
@	X	0.0
Static	C	57.0

true	Use criteria
1.8	Minimum +1 XCorr
2.5	Minimum +2 XCorr
3.5	Minimum +3 XCorr
0.08	Minimum DeltCN
1	Minimum charge state
3	Maximum charge state
0.0	Minimum ion proportion
1000	Maximum Sp rank
-1.0	Minimum Sp score
Include	Modified peptide inclusion
Any	Tryptic status requirement
true	Multiple, ambiguous IDs allowed
Ignore	Peptide validation handling
XCorr	Purge duplicate peptides by protein
false	Include only loci with unique peptide
false	Remove subset proteins
Ignore	Locus validation handling
0	Minimum modified peptides per locus
10	Minimum redundancy for low coverage loci
2	Minimum peptides per locus

Locus Key:

Validation Status

Locus

Sequence Count

Spectrum Count

Sequence Coverage

Length

MolWt

Descriptive Name

Similarity Key:

Locus

# of identical peptides

# of differing peptides

BIR1_YEAST

22.7%

954

108667

6.5

(P47134) BIR1 protein

	Filename	XCorr	DeltCN	ObsM+H+	CalcM+H+	SpR	SpScore	Ion%	#	Sequence
*	bir1-04.17740.17740.2	3.6693	0.3165	1412.09	1411.64	3	851.5	68.2%	3	K.LGFYFDPVIDPK.T	2
*	bir1-01.18368.18368.1	2.2316	0.0965	1290.67	1291.505	1	311.6	65.0%	1	K.DVLETLSNIMR.Q	1
*	bir1-01.18363.18363.2	4.0661	0.2842	1292.95	1291.505	1	1931.6	90.0%	1	K.DVLETLSNIMR.Q	2
*	bir1-06.33129.33129.3	4.0275	0.3444	2698.64	2697.926	1	1285.5	33.0%	1	R.KFTFQDNWPHSGSQNEHPLGIEK.M	3
*	bir1-02.07915.07915.2	3.0606	0.4972	1740.21	1742.88	1	579.2	62.5%	1	K.TLMNDTCYCIYCK.Q	2
*	bir1-02.06757.06757.2	3.9074	0.3974	2150.25	2150.282	1	1461.4	58.3%	1	K.EQINMENGSTTLEEGNINR.D	2
*	bir1-06.24881.24881.2	3.0541	0.2296	1401.75	1401.646	1	1131.0	77.3%	2	K.DLVIDFTSHIIK.N	2
*	bir1-06.27107.27107.3	5.001	0.3374	3662.78	3662.948	1	1053.1	28.1%	4	K.NAILDDSTDEFSFSNQGHNTFDIPIPTSSHLLK.G	3
*	bir1-06.27107.27107.2	3.0518	0.3619	2442.19	2441.706	1	294.5	31.0%	3	F.SFSNQGHNTFDIPIPTSSHLLK.G	3
*	bir1-01.19470.19470.2	4.6758	0.3889	1743.89	1744.852	1	2236.4	78.6%	3	K.FSVNSEEDLNFSEVK.L	2
*	bir1-06.02629.02629.2	2.7453	0.4062	1420.27	1419.624	1	382.4	54.2%	1	K.TSSIPADIHPKPR.K	2
*	bir1-02.11880.11880.2	4.0287	0.4167	2578.33	2578.81	1	765.5	40.9%	1	K.NETPNNEMLLFETGTPIASQENK.S	2
*	bir1-01.19511.19511.2	5.7381	0.506	2179.75	2181.486	1	2151.5	76.5%	5	K.QMPAEELDMTFNNWVNLK.V	2
*	bir1-01.19435.19435.3	4.6436	0.2735	2182.82	2181.486	1	866.1	44.1%	1	K.QMPAEELDMTFNNWVNLK.V	3
*	bir1-06.32527.32527.3	5.8567	0.3643	2863.94	2863.107	1	1397.1	40.2%	2	R.RDYYTATNFIETLEDDNQLIDIAK.K	3
*	bir1-06.30300.30300.3	5.056	0.2166	2992.31	2991.281	1	386.0	27.1%	2	R.RDYYTATNFIETLEDDNQLIDIAKK.M	3
*	bir1-04.25517.25517.2	5.5542	0.4874	2706.45	2706.92	1	1780.5	56.8%	15	R.DYYTATNFIETLEDDNQLIDIAK.K	2
*	bir1-01.17859.17859.3	6.5505	0.4831	2706.71	2706.92	1	1879.2	40.9%	6	R.DYYTATNFIETLEDDNQLIDIAK.K	3
*	bir1-06.33045.33045.3	4.1031	0.4379	2832.92	2835.094	1	625.3	28.3%	1	R.DYYTATNFIETLEDDNQLIDIAKK.M	3
*	bir1-01.19432.19432.2	2.9179	0.2723	2265.19	2265.479	1	327.3	39.5%	1	Y.TATNFIETLEDDNQLIDIAK.K	2
*	bir1-01.17880.17880.1	2.2619	0.1963	1029.81	1030.166	56	253.5	56.2%	1	D.DNQLIDIAK.K	1

HS75_YEAST

18.3%

612

66470

5.4

(P11484) Heat shock protein SSB1 (Cold-inducible protein YG101)

	Filename	XCorr	DeltCN	ObsM+H+	CalcM+H+	SpR	SpScore	Ion%	#	Sequence
	bir1-02.11374.11374.2	4.9816	0.4744	1731.65	1731.002	1	1804.1	70.6%	2	R.IINEPTAAAIAYGLGAGK.S	2 2
*	bir1-03.09414.09414.2	4.5841	0.4697	2463.87	2464.617	1	861.1	50.0%	3	R.TFTTCADNQTTVQFPVYQGER.V	2
	bir1-06.34964.34964.3	5.117	0.4581	2670.23	2670.154	1	1504.6	36.5%	2	K.NIPMMPAGEPVLEAIFEVDANGILK.V	3 3
	bir1-06.26316.26316.2	4.3648	0.2993	2451.11	2451.736	1	471.4	50.0%	1	R.QRLESYVASIEQTVTDPVLSSK.L	2 2
	bir1-06.26307.26307.3	4.9098	0.3838	2452.28	2451.736	1	828.1	32.1%	2	R.QRLESYVASIEQTVTDPVLSSK.L	3 3
	bir1-06.32868.32868.2	5.5472	0.3957	2627.79	2627.908	1	1400.6	52.1%	1	K.SKIEAALSDALAALQIEDPSADELR.K	2 2
	bir1-06.33015.33015.3	6.5473	0.4065	2628.26	2627.908	1	2217.5	41.7%	2	K.SKIEAALSDALAALQIEDPSADELR.K	3 3
	bir1-06.31044.31044.3	4.6095	0.3972	2757.05	2756.083	1	753.4	32.0%	4	K.SKIEAALSDALAALQIEDPSADELRK.A	3 3
	bir1-01.18764.18764.2	5.1799	0.4147	2411.71	2412.656	1	1621.3	54.5%	1	K.IEAALSDALAALQIEDPSADELR.K	2 2
	bir1-06.27869.27869.2	4.2878	0.3082	2540.13	2540.83	1	520.5	43.5%	1	K.IEAALSDALAALQIEDPSADELRK.A	2 2
	bir1-06.27822.27822.3	5.1362	0.4377	2541.5	2540.83	1	1003.5	38.0%	1	K.IEAALSDALAALQIEDPSADELRK.A	3 3

Similarities: HS76_YEAST(10:1)

HS76_YEAST

18.3%

612

66463

5.5

(P40150) Heat shock protein SSB2

	Filename	XCorr	DeltCN	ObsM+H+	CalcM+H+	SpR	SpScore	Ion%	#	Sequence
	bir1-02.11374.11374.2	4.9816	0.4744	1731.65	1731.002	1	1804.1	70.6%	2	R.IINEPTAAAIAYGLGAGK.S	2 2
*	bir1-02.09303.09303.2	4.8188	0.4517	2418.81	2419.61	1	865.3	47.5%	1	R.TFTTVSDNQTTVQFPVYQGER.V	2
	bir1-06.34964.34964.3	5.117	0.4581	2670.23	2670.154	1	1504.6	36.5%	2	K.NIPMMPAGEPVLEAIFEVDANGILK.V	3 3
	bir1-06.26316.26316.2	4.3648	0.2993	2451.11	2451.736	1	471.4	50.0%	1	R.QRLESYVASIEQTVTDPVLSSK.L	2 2
	bir1-06.26307.26307.3	4.9098	0.3838	2452.28	2451.736	1	828.1	32.1%	2	R.QRLESYVASIEQTVTDPVLSSK.L	3 3
	bir1-06.32868.32868.2	5.5472	0.3957	2627.79	2627.908	1	1400.6	52.1%	1	K.SKIEAALSDALAALQIEDPSADELR.K	2 2
	bir1-06.33015.33015.3	6.5473	0.4065	2628.26	2627.908	1	2217.5	41.7%	2	K.SKIEAALSDALAALQIEDPSADELR.K	3 3
	bir1-06.31044.31044.3	4.6095	0.3972	2757.05	2756.083	1	753.4	32.0%	4	K.SKIEAALSDALAALQIEDPSADELRK.A	3 3
	bir1-01.18764.18764.2	5.1799	0.4147	2411.71	2412.656	1	1621.3	54.5%	1	K.IEAALSDALAALQIEDPSADELR.K	2 2
	bir1-06.27869.27869.2	4.2878	0.3082	2540.13	2540.83	1	520.5	43.5%	1	K.IEAALSDALAALQIEDPSADELRK.A	2 2
	bir1-06.27822.27822.3	5.1362	0.4377	2541.5	2540.83	1	1003.5	38.0%	1	K.IEAALSDALAALQIEDPSADELRK.A	3 3

Similarities: HS75_YEAST(10:1)

HS72_YEAST

11.8%

638

69339

5.1

(P10592) Heat shock protein SSA2

Filename	XCorr	DeltCN	ObsM+H+	CalcM+H+	SpR	SpScore	Ion%	#	Sequence
bir1-01.19490.19490.2	6.3591	0.6479	2577.59	2578.792	1	2117.1	54.0%	1	R.SINPDEAVAYGAAVQAAILTGDESSK.T	2 2
bir1-06.34032.34032.2	5.257	0.4286	2556.51	2557.018	1	924.3	52.1%	1	K.TQDLLLLDVAPLSLGIETAGGVMTK.L	2 2
bir1-06.26619.26619.2	4.1896	0.3537	2715.53	2714.992	1	754.7	41.3%	1	K.KSEVFSTYADNQPGVLIQVFEGER.A	2

Similarities: HS71_YEAST(2:1)

HS71_YEAST

11.7%

641

69526

5.1

(P10591) Heat shock protein SSA1 (Heat shock protein YG100)

	Filename	XCorr	DeltCN	ObsM+H+	CalcM+H+	SpR	SpScore	Ion%	#	Sequence
	bir1-01.19490.19490.2	6.3591	0.6479	2577.59	2578.792	1	2117.1	54.0%	1	R.SINPDEAVAYGAAVQAAILTGDESSK.T	2 2
	bir1-06.34032.34032.2	5.257	0.4286	2556.51	2557.018	1	924.3	52.1%	1	K.TQDLLLLDVAPLSLGIETAGGVMTK.L	2 2
*	bir1-06.27606.27606.3	5.1192	0.3442	2730.65	2729.018	1	1168.4	32.6%	2	K.KSEIFSTYADNQPGVLIQVFEGER.A	3

Similarities: HS72_YEAST(2:1)

MPG1_YEAST

9.7%

361

39566

6.3

(P41940) Mannose-1-phosphate guanyltransferase (EC 2.7.7.13) (ATP-mannose-1-phosphate guanylyltransferase) (GDP-mannose pyrophosphorylase) (NDP-hexose pyrophosphorylase)

	Filename	XCorr	DeltCN	ObsM+H+	CalcM+H+	SpR	SpScore	Ion%	#	Sequence
*	bir1-03.19915.19915.2	2.8999	0.1375	2197.47	2197.583	8	262.2	34.2%	1	F.VGNRINAGLYILNPEVIDLI.E	2
*	bir1-01.18091.18091.2	2.9202	0.3802	1642.83	1641.905	2	330.4	46.4%	1	K.DFLSGTVLYLNSLAK.R	2

VATB_YEAST

8.9%

517

57749

5.1

(P16140) Vacuolar ATP synthase subunit B (EC 3.6.3.14) (V-ATPase B subunit) (Vacuolar proton pump B subunit) (V-ATPase 57 kDa subunit)

	Filename	XCorr	DeltCN	ObsM+H+	CalcM+H+	SpR	SpScore	Ion%	#	Sequence
*	bir1-02.10580.10580.2	4.0812	0.4016	1744.17	1743.954	1	1558.2	67.9%	2	R.LALTTAEYLAYQTER.H	2
*	bir1-06.28895.28895.2	5.0122	0.5281	1907.61	1907.192	1	1704.7	68.8%	1	R.HVLTILTDMSSYADALR.E	2
*	bir1-01.18838.18838.2	3.5627	0.388	1667.21	1665.886	1	1676.2	76.9%	2	R.TVFESLDQAWSLLR.I	2

YB06_YEAST

7.0%

698

79186

9.9

(P38283) Hypothetical 79.2 kDa protein in CNS1-CDC28 intergenic region

	Filename	XCorr	DeltCN	ObsM+H+	CalcM+H+	SpR	SpScore	Ion%	#	Sequence
*	bir1-06.26490.26490.3	5.1539	0.3745	2611.79	2613.798	1	1130.9	39.8%	9	R.SIIETLDDLNNLTTDAHSEINQR.L	3
*	bir1-06.26329.26329.2	5.5544	0.5257	2615.85	2613.798	1	1952.6	56.8%	3	R.SIIETLDDLNNLTTDAHSEINQR.L	2
*	bir1-06.28099.28099.3	5.4645	0.4025	2987.21	2986.357	1	562.8	26.0%	4	R.QQDINPQTIFGPIPPLHTDEIFPNPR.L	3

ETR1_YEAST

5.4%

371

40898

8.8

(P38071) Enoyl-[acyl-carrier protein] reductase [NADPH, B-specific] (EC 1.3.1.10) (Mitochondrial respiratory function protein 1)

	Filename	XCorr	DeltCN	ObsM+H+	CalcM+H+	SpR	SpScore	Ion%	#	Sequence
*	bir1-04.12528.12528.2	4.0948	0.2078	2211.91	2214.48	12	638.8	42.1%	19	Y.RVFSSSSDLIKVNDLDLFSA.A	2

DON1_YEAST

4.9%

365

40162

4.8

(Q05610) Donuts protein 1

	Filename	XCorr	DeltCN	ObsM+H+	CalcM+H+	SpR	SpScore	Ion%	#	Sequence
*	bir1-04.32947.32947.2	3.2241	0.1208	2211.97	2214.181	3	557.3	44.1%	9	S.NNLFDVLNCDESEEEEEQ.D	2
*	bir1-04.33463.33463.3	3.8442	0.0833	2213.84	2214.181	3	1340.2	38.2%	1	S.NNLFDVLNCDESEEEEEQ.D	3

YEB7_YEAST

123

4.7%

337

38235

5.0

(P39996) Hypothetical 38.2 kDa protein in PMP2-VAC8 intergenic region

	Filename	XCorr	DeltCN	ObsM+H+	CalcM+H+	SpR	SpScore	Ion%	#	Sequence
*	bir1-02.18767.18767.3	3.7711	0.1457	1801.04	1801.907	4	948.9	43.3%	6	N.SNAKTNFNLLDFSTDN.D	3
*	bir1-03.22604.22604.2	4.5511	0.2845	1803.23	1801.907	2	616.3	53.3%	117	N.SNAKTNFNLLDFSTDN.D	2

Q12482

4.4%

902

104304

8.0

(Q12482) Hypothetical protein YPR020C

	Filename	XCorr	DeltCN	ObsM+H+	CalcM+H+	SpR	SpScore	Ion%	#	Sequence
*	bir1-06.25415.25415.3	3.6761	0.1436	2710.19	2710.179	24	573.6	25.0%	1	G.KKDKATMRSDLTIEDFMKILNPN.Y	3
*	bir1-02.08567.08567.2	2.6661	0.0932	1900.69	1899.201	9	467.8	46.9%	1	I.QQANETATQIVKKLGLR.G	2

Q07442

4.2%

638

72513

6.1

(Q07442) BDF2 protein

	Filename	XCorr	DeltCN	ObsM+H+	CalcM+H+	SpR	SpScore	Ion%	#	Sequence
*	bir1-01.17392.17392.2	3.2788	0.2992	1701.87	1702.948	1	929.5	67.9%	1	D.LSLIETKLQGNVYHS.V	2
*	bir1-02.07177.07177.2	3.1846	0.1095	1277.05	1278.278	17	634.9	59.1%	1	S.TTNDNIGESGNR.E	2

DCP2_YEAST

3.0%

562

61781

6.4

(P16467) Pyruvate decarboxylase isozyme 2 (EC 4.1.1.1) (EC 4.1.1.-)

	Filename	XCorr	DeltCN	ObsM+H+	CalcM+H+	SpR	SpScore	Ion%	#	Sequence
*	bir1-06.42405.42405.2	3.267	0.2116	1838.77	1839.183	27	364.9	37.5%	19	R.TVVELIKDAKNPVILAD.A	2

YMH2_YEAST

2.7%

1545

171075

7.1

(Q03640) Hypothetical 171.1 kDa protein in RPL6A-DAK1 intergenic region

	Filename	XCorr	DeltCN	ObsM+H+	CalcM+H+	SpR	SpScore	Ion%	#	Sequence
*	bir1-06.27087.27087.2	3.1462	0.1532	1865.29	1867.158	17	441.5	40.6%	1	N.VFKKDPKSGKYVGNNGK.E	2
*	bir1-01.15048.15048.2	2.8662	0.2665	2563.79	2565.843	1	369.1	33.3%	1	L.GIAQINLGDPQIQQDGQISVKLGDG.H	2

DPP3_YEAST

2.4%

711

80510

6.3

(Q08225) Probable dipeptidyl-peptidase III (EC 3.4.14.4) (DPP III) (Dipeptidyl aminopeptidase III) (Dipeptidyl arylamidase III)

	Filename	XCorr	DeltCN	ObsM+H+	CalcM+H+	SpR	SpScore	Ion%	#	Sequence
*	bir1-03.20177.20177.2	3.7607	0.2068	1859.31	1858.058	2	379.6	43.8%	15	Q.GSKYFIDRSTVTPDLAS.L	2

YKM1_YEAST

1.9%

852

96022

8.4

(P32330) Hypothetical WD-repeats containing protein YKL121w

	Filename	XCorr	DeltCN	ObsM+H+	CalcM+H+	SpR	SpScore	Ion%	#	Sequence
*	bir1-06.23725.23725.2	3.0091	0.1052	1794.67	1797.122	8	431.1	46.7%	10	K.NGFLITASMDKTAKLW.H	2

KIN2_YEAST

1.6%

1147

128338

9.4

(P13186) Serine/threonine-protein kinase KIN2 (EC 2.7.1.37)

	Filename	XCorr	DeltCN	ObsM+H+	CalcM+H+	SpR	SpScore	Ion%	#	Sequence
*	bir1-04.25875.25875.3	3.7637	0.0865	2213.6	2215.516	1	1271.0	38.2%	1	I.IDFGLSNIFDYRKQLHTF.C	3
*	bir1-02.22261.22261.2	2.5748	0.1016	2217.59	2215.516	80	151.9	26.5%	1	I.IDFGLSNIFDYRKQLHTF.C	2

ARO1_YEAST

1.3%

1588

174754

6.3

(P08566) Pentafunctional AROM polypeptide [Includes: 3-dehydroquinate synthase (EC 4.2.3.4); 3-dehydroquinate dehydratase (EC 4.2.1.10) (3-dehydroquinase); Shikimate dehydrogenase (EC 1.1.1.25); Shikimate kinase (EC 2.7.1.71); 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19) (5-enolpyruvylshikimate-3-phosphate synthase) (EPSP synthase) (EPSPS)]

	Filename	XCorr	DeltCN	ObsM+H+	CalcM+H+	SpR	SpScore	Ion%	#	Sequence
*	bir1-06.39097.39097.2	3.048	0.1125	2213.97	2213.612	16	306.6	31.6%	19	N.FGGAAVTIPLKLDIMQYMDE.L	2

YKD8_YEAST

1.3%

1170

128240

7.9

(P32862) Putative 128.2 kDa transcriptional regulatory protein in PTM1-IXR1 intergenic region

	Filename	XCorr	DeltCN	ObsM+H+	CalcM+H+	SpR	SpScore	Ion%	#	Sequence
*	bir1-02.18691.18691.2	2.847	0.1753	1819.79	1821.046	4	428.3	50.0%	3	I.LRLNPTNSIDFNYRP.P	2
*	bir1-04.21463.21463.2	2.8192	0.1195	1708.21	1707.886	1	673.0	57.7%	1	L.RLNPTNSIDFNYRP.P	2

CAT8_YEAST

1.1%

1433

160485

9.0

(P39113) Regulatory protein CAT8

	Filename	XCorr	DeltCN	ObsM+H+	CalcM+H+	SpR	SpScore	Ion%	#	Sequence
*	bir1-06.27184.27184.2	3.9922	0.2556	1813.93	1811.989	1	872.3	56.7%	32	R.ITSEVALVHENALDNW.R	2

	Proteins	Peptide IDs	Copies
Unfiltered	6132	52914	175842
Redundant	21	77	383
Nonredundant	21	65	364

/data/2/shabadu/february2005/sandall/bir1