D noplus4

DTASelect v2.0.31
/data/3/taoxu/projects/catherine/jamie/mito/noplus4
/bluefish/people-b/applications/yates/dbase/SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta
ProLuCID 1.2.0 in SQT format.
--trypstat --modstat -p 1 -e cont -m 0 --fp 0.01
Jump to the summary table.

sequest.params modifications:

*	STY	79.9663
#	X	0.0
@	X	0.0
Static	C	57.0

true	Use criteria
0.0	Minimum peptide confidence
0.01	Peptide false positive rate
0.0	Minimum protein confidence
1.0	Protein false positive rate
1	Minimum charge state
16	Maximum charge state
0.0	Minimum ion proportion
1000	Maximum Sp rank
-1.0	Minimum Sp score
Require	Modified peptide inclusion
Any	Tryptic status requirement
false	Multiple, ambiguous IDs allowed
Ignore	Peptide validation handling
XCorr	Purge duplicate peptides by protein
false	Include only loci with unique peptide
true	Remove subset proteins
Ignore	Locus validation handling
cont	Exclude protein names matching
0	Minimum modified peptides per locus
1000	Minimum redundancy for low coverage loci
1	Minimum peptides per locus

Locus Key:

Validation Status

Locus

Sequence Count

Spectrum Count

Sequence Coverage

Length

MolWt

Descriptive Name

Similarity Key:

Locus

# of identical peptides

# of differing peptides

YKL042W

42.1%

363

42271

7.9

SPC42 SGDID:S000001525, Chr XI from 358119-359210, Verified ORF, "Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	Prob Score	Ion%	#	Sequence
*	Mito_110308.15312.15312.2	4.6664	0.398	100.0%	2608.2927	2607.0896	1	7.3079257	87.5%	3	R.LYDDYYNIPYQYSNPT#PMNR.D	2
*	Mito_110308.1804.1804.2	2.4725	0.2444	100.0%	1062.5127	1062.4503	1	5.600456	92.9%	1	K.LSDANST#FK.E	2
*	Mito_110308.11741.11741.2	3.277	0.3698	100.0%	2597.9128	2597.1477	1	6.008609	96.0%	2	K.VKDPMVDDDPVS#ENYDQINVPK.H	2
*	Mito_110308.11688.11688.3	4.047	0.215	100.0%	2598.8655	2597.1477	2	4.5244274	58.6%	1	K.VKDPMVDDDPVSENY#DQINVPK.H	3
*	Mito_110308.19243.19243.2	2.6778	0.382	100.0%	1377.8127	1376.6643	4	7.030125	70.6%	1	R.TLS#VLTNYVMR.S	2
*	Mito_110308.18819.18819.3	5.3761	0.3941	100.0%	2846.5454	2844.3345	1	7.4545937	39.7%	1	R.SEDGNNDRMS#PLPSPLNTILPINNR.L	3
*	Mito_110308.20328.20328.3	4.0862	0.4155	100.0%	2926.2854	2924.3008	1	6.8056173	40.3%	4	R.SEDGNNDRMS#PLPS#PLNTILPINNR.L	3
*	Mito_110308.22842.22842.2	4.7126	0.4821	100.0%	2038.9327	2036.9639	1	7.5221553	74.1%	3	R.MS#PLPS#PLNTILPINNR.L	2
*	Mito_110308.20108.20108.2	6.4831	0.4945	100.0%	2617.1528	2614.1887	1	9.482809	82.8%	1	R.KLS#LNNQLQELQSMMDGDDNIK.L	2
*	Mito_110308.19588.19588.3	5.4841	0.5489	100.0%	3273.3555	3270.538	1	9.117404	50.7%	1	R.KLS#LNNQLQELQSMMDGDDNIKLDNVSK.H	3
*	Mito_110308.10713.10713.2	5.6044	0.4811	100.0%	2336.5728	2332.862	2	8.266407	60.7%	3	R.DYS#PSSDACLECSNDLYEK.N	2
*	Mito_110308.1961.1961.2	4.9566	0.3756	100.0%	2417.1926	2412.8284	8	6.432883	42.9%	2	R.DYS#PSS#DACLECSNDLYEK.N	2
*	Mito_110308.8574.8574.3	3.4146	0.2968	100.0%	2228.5754	2226.9849	2	5.038301	50.0%	1	R.VKPENNMSETFAT#PTPNNR.-	3
*	Mito_110308.8658.8658.2	4.3957	0.3267	100.0%	2229.5728	2226.9849	1	6.6049204	60.7%	1	R.VKPENNMSETFAT#PTPNNR.-	2
*	Mito_110308.8834.8834.3	4.3557	0.2177	100.0%	2230.2253	2226.9849	8	5.7357535	46.0%	1	R.VKPENNMSETFATPT#PNNR.-	3
*	Mito_110308.8917.8917.2	3.8087	0.2383	100.0%	2230.6128	2226.9849	31	4.8761806	42.3%	1	R.VKPENNMSETFATPT#PNNR.-	2

YJR053W

27.4%

574

66087

5.9

BFA1 SGDID:S000003814, Chr X from 533941-535665, Verified ORF, "Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pathways that control exit from mitosis"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	Prob Score	Ion%	#	Sequence
*	Mito_110308.24277.24277.3	5.2353	0.5574	100.0%	4170.5356	4168.73	1	9.610499	46.6%	1	K.FSS#DDEGDFLTGFEELEGEAIDETISSNDKESADHPR.F	3
*	Mito_110308.25759.25759.3	4.0171	0.5217	100.0%	4250.845	4248.6963	1	8.072609	42.9%	1	K.FS#S#DDEGDFLTGFEELEGEAIDETISSNDKESADHPR.F	3
*	Mito_110308.11234.11234.2	3.7698	0.3179	100.0%	2484.0928	2482.249	1	5.0853457	87.5%	1	K.ISPAQYDIVKHDELLT#PGLHR.R	2
*	Mito_110308.10938.10938.2	3.0479	0.3829	100.0%	1848.1727	1847.7847	1	5.950899	77.3%	1	R.DWNTQQELDS#FKEK.R	2
*	Mito_110308.3954.3954.2	4.4913	0.3374	100.0%	1647.3528	1646.7104	1	6.2774043	73.9%	7	R.HCS#NQNVQLNGPAK.I	2
*	Mito_110308.8384.8384.2	3.1018	0.1016	100.0%	1936.3727	1934.8269	19	3.2230427	48.0%	1	K.YS#DLQHARATS#RNQK.V	2
*	Mito_110308.10443.10443.3	4.5291	0.4094	100.0%	2945.2454	2944.251	1	6.714648	50.0%	1	R.S#KS#QVNTPFVSNDNDGVYQSTAAQAR.L	3
*	Mito_110308.16318.16318.3	2.8903	0.3724	100.0%	3533.8755	3533.6755	38	5.8095098	25.3%	1	K.KLASWFIPRDET#IISVDEETIMDESTVNSK.R	3

YOR373W

25.7%

851

94104

7.0

NUD1 SGDID:S000005900, Chr XV from 1036830-1039385, Verified ORF, "Component of the spindle pole body outer plaque, required for exit from mitosis"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	Prob Score	Ion%	#	Sequence
*	Mito_110308.14526.14526.3	3.2029	0.3657	100.0%	3152.8455	3152.3367	1	5.458743	37.3%	1	K.EYESNHDFQDSNFTS#QVVEPAISDSVK.K	3
*	Mito_110308.12749.12749.3	3.9382	0.4037	100.0%	3153.8955	3152.3367	1	6.689379	44.8%	1	K.EYESNHDFQDSNFTSQVVEPAISDS#VK.K	3
*	Mito_110308.8911.8911.2	4.027	0.4802	100.0%	2039.6727	2037.9827	1	7.4977784	57.1%	1	K.KPPTMTVLNNYS#TVHQK.V	2
*	Mito_110308.9078.9078.3	2.5138	0.4424	100.0%	2100.0254	2098.9229	1	7.5208077	67.4%	1	K.VPSGFSGTTATS#HQEAQWK.Q	3
*	Mito_110308.15973.15973.2	3.2483	0.2485	100.0%	1404.1527	1403.6454	1	6.369433	85.0%	1	K.DLS#GVLETNTFK.R	2
*	Mito_110308.15829.15829.1	2.2181	0.2264	100.0%	1404.75	1403.6454	1	4.877962	87.5%	1	K.DLS#GVLETNTFK.R	1
*	Mito_110308.13284.13284.1	2.163	0.1651	100.0%	1560.83	1559.7465	1	4.0705366	89.5%	1	K.DLS#GVLETNTFKR.H	1
*	Mito_110308.7801.7801.3	3.3998	0.4457	100.0%	2249.4854	2247.97	1	6.6258163	63.9%	1	K.TTTMQTHENHDT#ISISHSK.D	3
*	Mito_110308.20084.20084.3	3.2997	0.3957	100.0%	2942.4854	2941.1812	2	6.9930196	36.2%	1	K.VSSSFS#DDS#DSGPAAEAHDVFDGILQK.Q	3
*	Mito_110308.22611.22611.3	3.5348	0.2703	100.0%	3022.7654	3021.1475	1	5.8349533	48.5%	1	K.VSSS#FS#DDS#DSGPAAEAHDVFDGILQK.Q	3
*	Mito_110308.9641.9641.2	2.8617	0.306	100.0%	1724.3727	1723.7323	1	5.80412	52.2%	1	K.SNYLVGSYPS#NSNNK.N	2
*	Mito_110308.9470.9470.1	2.084	0.1843	100.0%	1724.79	1723.7323	1	4.5069737	80.0%	1	K.SNYLVGSYPS#NSNNK.N	1
*	Mito_110308.9441.9441.3	3.8333	0.3529	100.0%	3878.9653	3875.6714	45	5.052724	26.6%	1	K.SNYLVGSYPSNSNNKNNNNNNNNNNNNS#ININNK.D	3
*	Mito_110308.6854.6854.2	4.048	0.2791	100.0%	2252.9727	2250.923	11	6.2146006	46.4%	7	K.NNNNNNNNNNNNS#ININNK.D	2
*	Mito_110308.1659.1659.3	4.8173	0.267	100.0%	2709.7153	2707.1313	1	5.4657197	55.9%	6	K.NNNNNNNNNNNNS#ININNKDNAR.T	3
*	Mito_110308.1178.1178.2	3.7798	0.4452	100.0%	2337.6926	2337.0493	1	7.634979	60.0%	1	K.KIQEEENLANSDDT#PLDTPK.F	2
*	Mito_110308.1365.1365.3	4.7667	0.2664	100.0%	2339.8154	2337.0493	1	5.3805523	64.2%	1	K.KIQEEENLANS#DDTPLDTPK.F	3
*	Mito_110308.14528.14528.3	6.637	0.483	100.0%	3205.2554	3202.4827	1	8.266667	51.6%	1	K.KIQEEENLANSDDTPLDT#PKFNDLFTK.N	3
*	Mito_110308.1181.1181.2	5.1745	0.4866	100.0%	2209.3728	2208.9543	1	8.681104	82.8%	1	K.IQEEENLANSDDTPLDT#PK.F	2
*	Mito_110308.1087.1087.3	3.9235	0.3695	100.0%	2210.0054	2208.9543	1	5.2979965	56.0%	1	K.IQEEENLANSDDT#PLDTPK.F	3
*	Mito_110308.16064.16064.3	5.0006	0.3746	100.0%	3075.2056	3074.3877	1	7.0842	47.7%	1	K.IQEEENLANSDDTPLDT#PKFNDLFTK.N	3
*	Mito_110308.16096.16096.2	3.3359	0.3475	100.0%	3078.5728	3074.3877	7	5.66294	50.0%	2	K.IQEEENLANSDDTPLDT#PKFNDLFTK.N	2
*	Mito_110308.16820.16820.3	5.2511	0.5017	100.0%	3155.7554	3154.354	1	8.851007	54.5%	1	K.IQEEENLANSDDT#PLDT#PKFNDLFTK.N	3
*	Mito_110308.18840.18840.3	4.6677	0.4181	100.0%	3237.0554	3234.3203	1	5.8852234	48.3%	1	K.IQEEENLANS#DDT#PLDT#PKFNDLFTK.N	3
*	Mito_110308.13262.13262.2	3.0345	0.4579	100.0%	2303.0327	2301.941	1	6.9336853	65.4%	1	R.VEDITS#ISEVNTS#FNETEK.Q	2
*	Mito_110308.13514.13514.2	4.9675	0.4884	100.0%	2303.6328	2301.941	1	8.845834	76.0%	1	R.VEDITSIS#EVNTS#FNETEK.Q	2
*	Mito_110308.1954.1954.2	3.584	0.2784	100.0%	2383.4727	2381.9072	1	5.5226045	70.8%	1	R.VEDITS#IS#EVNTS#FNETEK.Q	2
*	Mito_110308.9610.9610.2	3.6547	0.252	100.0%	1550.8528	1550.6046	1	6.5999374	90.0%	1	K.LSGS#PSYDSDWEK.I	2
*	Mito_110308.9713.9713.1	2.3253	0.3144	100.0%	1553.0	1550.6046	4	5.18837	64.7%	2	K.LSGS#PSYDSDWEK.I	1

YPL124W

20.9%

253

29280

9.5

SPC29 SGDID:S000006045, Chr XVI from 316754-317515, Verified ORF, "Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inner plaque component Spc110p; required for SPB duplication"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	Prob Score	Ion%	#	Sequence
*	Mito_110308.7245.7245.2	3.3672	0.2208	100.0%	1217.6727	1216.5358	5	5.239404	86.7%	13	K.KFQDDT#LNR.V	2
*	Mito_110308.1291.1291.2	2.6377	0.2105	100.0%	1088.6927	1088.4408	1	4.7111754	92.3%	6	K.FQDDT#LNR.V	2
*	Mito_110308.8292.8292.3	4.693	0.2741	100.0%	2034.3254	2032.9335	1	5.6592655	63.4%	1	K.KLREENFSSNTS#ELGNK.K	3
*	Mito_110308.1928.1928.2	3.2586	0.3359	100.0%	1906.4127	1904.8385	5	6.1676245	44.0%	1	K.LREENFSSNT#SELGNK.K	2
*	Mito_110308.2581.2581.2	2.6356	0.1962	100.0%	1111.2327	1109.5602	1	5.4296	92.9%	1	R.KVKS#PLDDK.L	2
*	Mito_110308.7981.7981.2	2.531	0.165	100.0%	983.1727	981.4652	6	4.829396	84.6%	1	K.VKS#PLDDK.L	2
*	Mito_110308.7890.7890.1	1.5463	0.4435	100.0%	1250.61	1250.6504	13	6.4360876	64.3%	1	K.VKS#PLDDKLR.R	1
*	Mito_110308.9926.9926.2	5.107	0.4602	100.0%	1923.8528	1922.8743	1	8.498767	91.7%	4	R.SSQIHIENEST#EDILK.I	2
*	Mito_110308.9829.9829.3	3.7205	0.3469	100.0%	1923.8655	1922.8743	2	5.487878	75.0%	1	R.SSQIHIENEST#EDILK.I	3
*	Mito_110308.1946.1946.2	2.9918	0.2017	100.0%	2003.6327	2002.8406	1	4.6994457	64.0%	1	R.SSQIHIENES#T#EDILK.I	2

YIL002W-A

18.8%

7729

4.7

YIL002W-A SGDID:S000028835, Chr IX from 350298-350507, Uncharacterized ORF, "Identified by expression profiling and mass spectrometry"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	Prob Score	Ion%	#	Sequence
*	Mito_110308.13291.13291.1	2.4535	0.3114	100.0%	1648.73	1648.7764	1	5.5729485	94.1%	1	K.MDS#LLVQLEDLHR.D	1
*	Mito_110308.13202.13202.2	3.3585	0.1	100.0%	1651.9927	1648.7764	15	4.025297	55.0%	2	K.MDS#LLVQLEDLHR.D	2

YNL225C

17.4%

581

67400

6.0

CNM67 SGDID:S000005169, Chr XIV from 224470-222725, reverse complement, Verified ORF, "Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	Prob Score	Ion%	#	Sequence
*	Mito_110308.13508.13508.3	3.3849	0.2731	100.0%	3296.8755	3295.5266	13	4.8208375	31.6%	1	R.LDSPVS#ENGEIKDGEPIPQNWLNENHVGK.S	3
*	Mito_110308.13007.13007.3	4.3161	0.3074	100.0%	3296.9055	3295.5266	1	5.463965	35.2%	1	R.LDS#PVSENGEIKDGEPIPQNWLNENHVGK.S	3
*	Mito_110308.14531.14531.3	4.9891	0.2941	100.0%	3379.9753	3375.493	41	6.0222993	30.0%	3	R.LDS#PVS#ENGEIKDGEPIPQNWLNENHVGK.S	3
*	Mito_110308.8833.8833.3	3.4727	0.3506	100.0%	2246.9355	2244.8572	1	5.8911505	63.6%	1	R.DSYEENKSPS#MDQMNYAR.N	3
*	Mito_110308.1658.1658.2	2.9857	0.1034	99.1%	1815.1927	1813.8115	2	4.371732	68.2%	1	R.NTSYQES#PGLQERPK.N	2
*	Mito_110308.1315.1315.2	2.8581	0.2453	100.0%	1277.1527	1276.5933	1	4.529336	94.4%	3	K.DKS#PIGTDVHK.K	2
*	Mito_110308.1856.1856.2	2.898	0.3092	100.0%	1405.3127	1404.6882	5	4.583086	60.0%	1	K.DKS#PIGTDVHKK.D	2
*	Mito_110308.1423.1423.3	4.8963	0.3718	100.0%	2968.4055	2965.2153	13	8.799373	38.6%	1	R.ANEQASAQPTDEHTSPDIS#IEDCNGAK.I	3

YLR445W

14.4%

188

22365

4.9

YLR445W SGDID:S000004437, Chr XII from 1024185-1024568,1024651-1024833, Uncharacterized ORF, "Hypothetical protein"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	Prob Score	Ion%	#	Sequence
*	Mito_110308.17365.17365.2	3.345	0.2694	100.0%	3315.4326	3312.3452	20	4.285797	41.7%	1	T.T#EVPRQSS#ENDQDNNLERTNS#LKSPDV.T	2

YPR103W

14.3%

287

31637

6.2

PRE2 SGDID:S000006307, Chr XVI from 732347-733210, Verified ORF, "20S proteasome beta-type subunit, responsible for the chymotryptic activity of the proteasome"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	Prob Score	Ion%	#	Sequence
*	Mito_110308.34435.34435.3	3.5113	0.3214	100.0%	4930.615	4925.027	8	5.066011	30.9%	1	R.DAYSGGS#VNLYHVTEDGWIY#HGNHDVGELFWKVKEEEGS#FN.N	3

YOR257W

11.8%

161

18751

4.6

CDC31 SGDID:S000005783, Chr XV from 811006-811491, Verified ORF, "Component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; interacts with Kar1p"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	Prob Score	Ion%	#	Sequence
*	Mito_110308.10972.10972.2	4.9342	0.4987	100.0%	2210.7927	2209.0496	1	7.8848934	67.9%	1	K.NRSS#LQSGPLNSELLEEQK.Q	2

YPL131W

11.1%

297

33715

6.8

RPL5 SGDID:S000006052, Chr XVI from 303120-304013, Verified ORF, "Protein component of the large (60S) ribosomal subunit with similarity to E. coli L18 and rat L5 ribosomal proteins; binds 5S rRNA and is required for 60S subunit assembly"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	Prob Score	Ion%	#	Sequence
*	Mito_110308.18606.18606.3	4.2866	0.3538	100.0%	3981.9854	3978.7356	2	6.538453	32.9%	1	D.EERFS#ELFKGY#LADDIDADSLEDIYTSAHEAIR.A	3

YPL255W

10.4%

385

45384

6.5

BBP1 SGDID:S000006176, Chr XVI from 67725-68882, Verified ORF, "Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periphery; forms a complex with a nuclear envelope protein Mps2p and SPB components Spc29p and Kar1p; required for mitotic functions of Cdc5p"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	Prob Score	Ion%	#	Sequence
*	Mito_110308.15210.15210.2	2.4444	0.2999	100.0%	1461.4127	1461.633	1	5.8239408	80.0%	1	K.DALFGTDIS#PSMK.Y	2
*	Mito_110308.1352.1352.2	3.4015	0.2048	100.0%	1481.4528	1479.4899	1	5.1326556	88.9%	1	R.YAQDDT#NFS#MK.F	2
*	Mito_110308.9562.9562.3	3.0885	0.2449	100.0%	1850.8455	1849.8691	1	5.5750675	65.9%	1	K.VLDQTISHEAELATS#R.E	3

YAL047C

10.0%

622

72105

5.1

SPC72 SGDID:S000000045, Chr I from 56858-54990, reverse complement, Verified ORF, "Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them to microtubules; has roles in astral microtubule formation and stabilization"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	Prob Score	Ion%	#	Sequence
*	Mito_110308.21190.21190.3	4.1866	0.2033	100.0%	2330.7854	2329.1492	64	4.947989	39.6%	1	R.FEKT#LDT#QLEIVIEILHK.E	3
*	Mito_110308.30540.30540.3	3.427	0.3042	100.0%	3596.8455	3594.7532	38	5.442783	29.4%	1	R.FEKT#LDT#QLEIVIEILHKEYDQFINSIR.L	3
*	Mito_110308.21913.21913.2	4.5268	0.1355	100.0%	1847.6527	1844.9768	14	4.2040453	54.2%	1	K.TLDT#QLEIVIEILHK.E	2
*	Mito_110308.31565.31565.3	4.7961	0.3373	100.0%	3113.4255	3110.5808	27	6.2363014	33.9%	1	K.TLDT#QLEIVIEILHKEYDQFINSIR.L	3
*	Mito_110308.15001.15001.3	5.2763	0.378	100.0%	2693.8455	2693.2551	1	6.5325227	60.0%	1	K.LNEQSHLLDSLELEENSSS#VIEK.Q	3
*	Mito_110308.15058.15058.2	5.5162	0.3855	100.0%	2696.7126	2693.2551	5	7.082342	51.7%	1	K.LNEQSHLLDSLELEENSSS#VIEK.Q	2
*	Mito_110308.16467.16467.2	3.2146	0.375	100.0%	1327.1127	1326.6453	1	6.0787835	88.2%	1	R.SKLFNLS#INNP.-	2

YDR356W

8.8%

944

111782

7.1

SPC110 SGDID:S000002764, Chr IV from 1186098-1188932, Verified ORF, "Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	Prob Score	Ion%	#	Sequence
*	Mito_110308.16474.16474.2	3.4589	0.3046	100.0%	1363.9528	1362.6163	1	6.3449035	88.9%	1	K.NMEFT#PVGFIK.S	2
*	Mito_110308.8397.8397.3	4.3543	0.3541	100.0%	3077.5754	3074.4539	3	6.4282417	39.7%	1	K.RNTTQT#QVVSPTKVPNANNGDENEGPVK.K	3
*	Mito_110308.2366.2366.2	2.7008	0.3768	100.0%	1383.5327	1383.6515	2	7.2587047	65.0%	1	R.NT#TQTQVVSPTK.V	2
*	Mito_110308.2108.2108.2	3.6489	0.3451	100.0%	1383.6327	1383.6515	1	5.946111	80.0%	1	R.NTT#QTQVVSPTK.V	2
*	Mito_110308.1335.1335.2	2.4749	0.2129	100.0%	1383.8528	1383.6515	1	4.73857	70.0%	1	R.NTTQTQVVS#PTK.V	2
*	Mito_110308.18323.18323.2	2.7558	0.2201	100.0%	1940.5728	1939.836	1	4.944556	87.5%	1	R.LFSEAS#QFDDSFPEIK.A	2
*	Mito_110308.17315.17315.2	4.1165	0.1612	100.0%	1942.2927	1939.836	1	5.7788243	60.9%	2	R.LFSEASQFDDS#FPEIK.A	2
*	Mito_110308.19590.19590.2	3.7535	0.4095	100.0%	2021.8328	2019.8024	1	6.6598935	64.0%	1	R.LFSEAS#QFDDS#FPEIK.A	2
*	Mito_110308.6168.6168.2	2.1547	0.1995	99.1%	931.8927	931.4397	4	4.3845677	76.9%	2	K.ANIPPS#PR.S	2
*	Mito_110308.17847.17847.2	3.8504	0.2715	100.0%	2432.0127	2430.166	7	4.851539	48.1%	1	K.SEQS#NIQHDLNLQILNLENK.L	2

YOR284W

8.6%

243

28414

9.0

HUA2 SGDID:S000005810, Chr XV from 848475-849206, Uncharacterized ORF, "Cytoplasmic protein of unknown function; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	Prob Score	Ion%	#	Sequence
*	Mito_110308.13526.13526.2	2.4126	0.3289	100.0%	2675.7327	2674.0303	1	5.7185373	66.7%	1	K.MYLDHDS#VVEYS#EDEIVEADR.I	2

YLR212C

7.8%

473

52628

4.7

TUB4 SGDID:S000004202, Chr XII from 566283-564862, reverse complement, Verified ORF, "Gamma-tubulin, involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	Prob Score	Ion%	#	Sequence
*	Mito_110308.27256.27256.3	3.8479	0.3187	100.0%	4339.7056	4336.8345	1	4.928109	36.8%	1	R.S#PY#LPLQPNENEVSGMMLSNMSTVVNVFENACNTFDK.V	3

YIL149C

7.5%

1679

195140

6.0

MLP2 SGDID:S000001411, Chr IX from 68067-63028, reverse complement, Verified ORF, "Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved in the Tel1p pathway that controls telomere length"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	Prob Score	Ion%	#	Sequence
*	Mito_110308.9330.9330.3	4.1571	0.3619	100.0%	2072.9653	2070.9856	1	6.886935	63.0%	1	K.TKS#SLYSAQDLLDKHER.K	3
*	Mito_110308.9150.9150.3	3.8159	0.3225	100.0%	2841.3855	2843.357	12	6.5899186	33.3%	1	N.EISDLKGKLS#SAENANADLENKFNR.L	3
*	Mito_110308.16573.16573.3	3.6367	0.3202	100.0%	3185.1555	3184.303	1	5.1710954	40.3%	1	K.ENAGSLTFLDNKGS#GEDAEEELWNS#PSK.G	3
*	Mito_110308.10674.10674.2	5.1501	0.4032	100.0%	1816.2927	1814.7115	2	7.0548086	73.9%	2	K.GSGEDAEEELWNSPS#K.G	2
*	Mito_110308.13504.13504.3	4.1761	0.4269	100.0%	2989.3455	2987.269	1	8.165093	52.5%	1	K.GSGEDAEEELWNS#PSKGNSERPSAVAGF.I	3
*	Mito_110308.12754.12754.3	4.2912	0.4591	100.0%	3472.9753	3470.5496	2	6.7691236	44.9%	2	K.GSGEDAEEELWNS#PSKGNSERPSAVAGFINQK.N	3
*	Mito_110308.9961.9961.3	3.5964	0.3604	100.0%	4481.9956	4485.1353	1	6.4758387	34.1%	1	K.NLKPQEQLKNVKNDVS#FNDSQSMVTNKENNIVDSSAAGNK.A	3

YNL188W

6.2%

433

50653

9.3

KAR1 SGDID:S000005132, Chr XIV from 286309-287610, Verified ORF, "Essential protein involved in karyogamy during mating and in spindle pole body duplication during mitosis, localizes to the half-bridge of the spindle pole body, interacts with Spc72p during karyogamy, also interacts with Cdc31p"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	Prob Score	Ion%	#	Sequence
*	Mito_110308.17074.17074.3	4.69	0.4099	100.0%	3280.7354	3277.4307	1	6.6671085	36.9%	1	K.AEEYIS#DEDNVKIDEDNIENELQFTPK.I	3

YLR457C

5.3%

319

37354

10.2

NBP1 SGDID:S000004449, Chr XII from 1056768-1055809, reverse complement, Verified ORF, "Component of the mitotic apparatus containing a coiled-coil domain, essential for the G2/M transition"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	Prob Score	Ion%	#	Sequence
*	Mito_110308.15039.15039.2	2.9756	0.218	100.0%	2026.6527	2024.9575	1	5.626281	72.0%	1	R.ILEDLLDSS#NIHPSYTK.S	2

YGL219C

5.2%

459

51990

9.0

MDM34 SGDID:S000003187, Chr VII from 84256-82877, reverse complement, Verified ORF, "Mitochondrial outer membrane protein, required for normal mitochondrial morphology and inheritance; localizes to dots on the mitochondrial surface near mtDNA nucleoids; interacts genetically with MDM31 and MDM32"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	Prob Score	Ion%	#	Sequence
*	Mito_110308.24006.24006.3	3.4955	0.2497	100.0%	2827.6455	2824.1936	7	4.7748375	34.4%	1	R.MPSLNNYYS#S#QSFPQPKSSTVSSK.Q	3

YDR320C

5.1%

668

75020

5.3

SWA2 SGDID:S000002728, Chr IV from 1108096-1106090, reverse complement, Verified ORF, "Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	Prob Score	Ion%	#	Sequence
*	Mito_110308.38281.38281.3	3.1246	0.2707	100.0%	3718.6455	3724.5774	39	4.7817836	26.8%	1	S.KET#TPQSSNS#PSITGSAVADVARTDKSPNDS#LHS.I	3

YFR028C

4.7%

551

61907

8.0

CDC14 SGDID:S000001924, Chr VI from 210056-208401, reverse complement, Verified ORF, "Protein phosphatase required for mitotic exit; located in the nucleolus until liberated by the FEAR and Mitotic Exit Network in anaphase, enabling it to act on key substrates to effect a decrease in CDK/B-cyclin activity and mitotic exit"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	Prob Score	Ion%	#	Sequence
*	Mito_110308.8390.8390.3	4.9655	0.4818	100.0%	2990.4556	2989.2517	16	7.3288693	33.8%	1	R.KVVIES#NNSDDESMQDTNGTSNHYPK.V	3

YIL041W

4.6%

326

36670

5.0

GVP36 SGDID:S000001303, Chr IX from 276524-277504, Uncharacterized ORF, "Golgi vesicle protein of unknown function; localizes to both early and late Golgi vesicles"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	Prob Score	Ion%	#	Sequence
*	Mito_110308.8178.8178.1	1.8214	0.2401	100.0%	1785.97	1785.8993	19	4.2443314	50.0%	1	S.KKLQEISTS#VSEKTQ.E	1

YGR132C

4.2%

287

31427

8.2

PHB1 SGDID:S000003364, Chr VII from 756457-755594, reverse complement, Verified ORF, "Subunit of the prohibitin complex (Phb1p-Phb2p), a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilizes newly synthesized proteins; determinant of replicative life span; involved in mitochondrial segregation"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	Prob Score	Ion%	#	Sequence
*	Mito_110308.28143.28143.2	2.9442	0.2657	100.0%	1326.5527	1324.6912	1	4.9026527	70.0%	1	A.LPIGIIASGIQY#.S	2

YNL009W

3.3%

420

47856

9.2

IDP3 SGDID:S000004954, Chr XIV from 614822-616084, Verified ORF, "Peroxisomal NADP-dependent isocitrate dehydrogenase, catalyzes oxidation of isocitrate to alpha-ketoglutarate with the formation of NADP(H+), required for growth on unsaturated fatty acids"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	Prob Score	Ion%	#	Sequence
*	Mito_110308.11200.11200.2	3.6325	0.0524	100.0%	1897.4927	1896.8778	6	2.9290097	61.9%	2	K.QIFDNLFDKEY#KEK.F	2

YHR172W

3.2%

823

96825

6.4

SPC97 SGDID:S000001215, Chr VIII from 448335-450806, Verified ORF, "Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	Prob Score	Ion%	#	Sequence
*	Mito_110308.13516.13516.2	4.2027	0.3643	100.0%	2259.8928	2258.8843	1	6.780888	65.4%	1	R.RTNQSS#QEDFNNFMDSMK.N	2
*	Mito_110308.16067.16067.3	3.5545	0.4614	100.0%	3040.7954	3039.2607	1	6.75281	40.3%	1	R.TNQSS#QEDFNNFMDSMKNESSLHLR.L	3

YPL109C

3.2%

657

76176

8.4

YPL109C SGDID:S000006030, Chr XVI from 347388-345596,345443-345263, reverse complement, Uncharacterized ORF, "Hypothetical protein"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	Prob Score	Ion%	#	Sequence
*	Mito_110308.20054.20054.3	3.1749	0.2972	100.0%	2654.8755	2659.272	13	4.8634276	33.3%	1	I.RQFIIPTSIAFY#LTQNS#FPKQ.N	3

YLR353W

3.2%

603

66289

6.3

BUD8 SGDID:S000004345, Chr XII from 834351-836162, Verified ORF, "Protein involved in bud-site selection; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern, and bud at the proximal pole"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	Prob Score	Ion%	#	Sequence
*	Mito_110308.1838.1838.3	3.2509	0.271	100.0%	1925.7854	1924.8032	30	4.599766	41.2%	1	K.SSTSDDSHSPGAPGTS#ARK.I	3

YGL075C

2.8%

387

44585

8.3

MPS2 SGDID:S000003043, Chr VII from 368091-366928, reverse complement, Verified ORF, "Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required for insertion of the newly duplicated SPB into the nuclear envelope; potentially phosphorylated by Cdc28p"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	Prob Score	Ion%	#	Sequence
*	Mito_110308.2851.2851.2	3.3191	0.4403	100.0%	1182.8328	1182.515	1	8.540999	77.8%	3	K.NTEGAGIST#PR.K	2

YPL061W

2.6%

500

54414

5.4

ALD6 SGDID:S000005982, Chr XVI from 432585-434087, Verified ORF, "Cytosolic aldehyde dehydrogenase that is activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for the conversion of acetaldehyde to acetate; constitutively expressed"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	Prob Score	Ion%	#	Sequence
*	Mito_110308.11912.11912.2	3.0524	0.0884	100.0%	1524.3927	1523.6389	3	3.2893052	70.0%	1	K.SVAVDS#S#ESNLKK.I	2

YMR001C

2.3%

705

81031

9.0

CDC5 SGDID:S000004603, Chr XIII from 271136-269019, reverse complement, Verified ORF, "Polo-like kinase with similarity to Xenopus Plx1 and S. pombe Plo1p; found at bud neck, nucleus and SPBs; has multiple functions in mitosis and cytokinesis through phosphorylation of substrates; may be a Cdc28p substrate"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	Prob Score	Ion%	#	Sequence
*	Mito_110308.8526.8526.3	2.7954	0.3212	100.0%	1744.4054	1742.92	1	6.108603	72.5%	1	K.LVHT#PIKGNTADLVGK.E	3

U	YDL080C	1	1	2.1%	609	68366	6.4	THI3 SGDID:S000002238, Chr IV from 312471-310642, reverse complement, Verified ORF, "Probable decarboxylase, required for expression of enzymes involved in thiamine biosynthesis; may have a role in catabolism of amino acids to long-chain and complex alcohols"
U	YLR134W	1	1	2.3%	563	61912	6.4	PDC5 SGDID:S000004124, Chr XII from 410724-412415, Verified ORF, "Minor isoform of pyruvate decarboxylase, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde, regulation is glucose- and ethanol-dependent, repressed by thiamine, involved in amino acid catabolism"
U	YLR044C	1	1	2.3%	563	61495	6.2	PDC1 SGDID:S000004034, Chr XII from 234082-232391, reverse complement, Verified ORF, "Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde; subject to glucose-, ethanol-, and autoregulation; involved in amino acid catabolism"
U	YGR087C	1	1	2.3%	563	61581	6.2	PDC6 SGDID:S000003319, Chr VII from 652985-651294, reverse complement, Verified ORF, "Minor isoform of pyruvate decarboxylase, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde, regulation is glucose- and ethanol-dependent, involved in amino acid catabolism"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	Prob Score	Ion%	#	Sequence
	Mito_110308.12351.12351.3	2.674	0.3545	100.0%	1581.3555	1580.7006	1	5.3173428	64.9%	1	H.HT#LGNGDFTVFHR.I	3

YLL003W

2.0%

946

112978

9.8

SFI1 SGDID:S000003926, Chr XII from 143200-146040, Verified ORF, "Centrin (Cdc31p)-binding protein required for spindle pole body (SPB) duplication, localizes to the half-bridge of the SPB, required for progression through G(2)-M transition, has similarity to Xenopus laevis XCAP-C"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	Prob Score	Ion%	#	Sequence
*	Mito_110308.15302.15302.2	3.3383	0.2741	100.0%	2276.7527	2274.0706	10	5.4563956	46.2%	1	R.DKFVPETSPT#NIPT#DVLIK.Q	2

Reverse_YPL195W

1.8%

932

106924

5.1

APL5 SGDID:S000006116, Chr XVI from 176222-179020, Verified ORF, "Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	Prob Score	Ion%	#	Sequence
*	Mito_110308.1173.1173.2	3.5061	0.0244	99.1%	2124.4727	2123.878	1	2.868129	55.6%	1	K.KDS#SEEENLDLLDKS#NK.T	2

YML031W

1.7%

655

74134

8.3

NDC1 SGDID:S000004493, Chr XIII from 214189-216156, Verified ORF, "Nuclear envelope protein with multiple putative transmembrane domains, required for nuclear pore complex assembly and spindle pole body duplication; required for meiosis II"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	Prob Score	Ion%	#	Sequence
*	Mito_110308.8815.8815.2	3.229	0.3437	100.0%	1368.7327	1367.6678	1	4.9905233	100.0%	1	R.VRTDDS#VLLNR.G	2

YNL163C

1.4%

1110

124464

5.1

RIA1 SGDID:S000005107, Chr XIV from 330075-326743, reverse complement, Verified ORF, "Cytoplasmic GTPase involved in biogenesis of the 60S ribosome; has similarity to translation elongation factor 2 (Eft1p and Eft2p)"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	Prob Score	Ion%	#	Sequence
*	Mito_110308.10897.10897.2	2.3646	0.2959	100.0%	1595.6727	1593.8975	22	5.6831746	41.7%	1	R.GLAGKVLKS#GTLIEK.G	2

YMR302C

0.9%

850

96689

8.9

PRP12 SGDID:S000004917, Chr XIII from 872624-870072, reverse complement, Verified ORF, "Integral inner mitochondrial membrane protein with similarity to exonucleases; prp12 mutants exhibit an increased rate of mt DNA escape"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	Prob Score	Ion%	#	Sequence
*	Mito_110308.2056.2056.2	2.1449	0.2323	100.0%	1038.5127	1037.4064	11	4.021295	71.4%	1	R.T#T#SLNVSR.M	2

	Proteins	Peptide IDs	Spectra
Unfiltered	10495	42334	46670
Filtered	36	127	189
Forward matches	35	126	188
Decoy matches	1	1	1
Forward FP rate	2.86%	0.79%	0.53%

/data/3/taoxu/projects/catherine/jamie/mito/noplus4