D step

DTASelect v2.0.9
/data/1/catclw/Projects/Jonathan/step_2-5
/scratch/yates/SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta
SEQUEST 3.0 in SQT format.

Jump to the summary table.

sequest.params modifications:

*	STY	80.0
#	X	0.0
@	X	0.0
Static	C	57.0

true	Use criteria
0.0	Minimum peptide confidence
0.05	Peptide false positive rate
0.0	Minimum protein confidence
1.0	Protein false positive rate
1	Minimum charge state
9	Maximum charge state
0.0	Minimum ion proportion
1000	Maximum Sp rank
-1.0	Minimum Sp score
Include	Modified peptide inclusion
Any	Tryptic status requirement
false	Multiple, ambiguous IDs allowed
Ignore	Peptide validation handling
XCorr	Purge duplicate peptides by protein
false	Include only loci with unique peptide
true	Remove subset proteins
Ignore	Locus validation handling
0	Minimum modified peptides per locus
1000	Minimum redundancy for low coverage loci
2	Minimum peptides per locus

Locus Key:

Validation Status

Locus

Sequence Count

Spectrum Count

Sequence Coverage

Length

MolWt

Descriptive Name

Similarity Key:

Locus

# of identical peptides

# of differing peptides

YKL089W

114

19.9%

549

62473

4.8

MIF2 SGDID:S000001572, Chr XI from 273038-274687, Verified ORF, "Kinetochore protein with homology to human CENP-C, required for structural integrity of the spindle during anaphase spindle elongation, interacts with histones H2A, H2B, and H4, phosphorylated by Ipl1p"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	Mif2_02_itms.14255.14255.2	3.0049	0.1907	96.4%	2801.7322	2802.0103	1	5.063	31.8%	1	K.DEYSMENIDDFFKDDETSLISMR.R	2
*	Mif2_03_itms.08329.08329.2	2.8915	0.3487	100.0%	2132.2722	2133.057	8	5.914	47.1%	3	K.NVYS*DVPDLVADEDDDDR.V	2
*	Mif2_02_itms.06572.06572.2	3.606	0.1804	99.0%	2110.4722	2111.2236	2	5.349	50.0%	2	K.IVTYDESEDEEEILAAQR.R	2
*	Mif2_02_itms.04809.04809.2	3.2242	0.1919	97.6%	1934.6522	1934.151	1	4.529	56.2%	2	A.NVKISDTKEETKDEIIA.F	2
*	Mif2_02_itms.10947.10947.2	5.0803	0.3789	100.0%	1816.8522	1817.0204	1	7.899	75.0%	24	K.DTKDENFALEIMFDK.H	2
*	Mif2_02_itms.12713.12713.2	5.3835	0.1434	100.0%	1473.8722	1472.6527	1	6.442	86.4%	50	K.DENFALEIMFDK.H	2
*	Mif2_02_itms.08751.08751.2	4.4969	0.3879	100.0%	2075.7522	2075.211	1	8.472	58.8%	32	K.MFFVQVTVSEDANDDNDK.E	2

contaminant_KERATIN02

11.4%

622

61987

5.2

no description

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	Mif2_03_itms.04687.04687.2	3.6319	0.3269	100.0%	1233.7522	1233.2833	1	7.74	70.0%	9	T.SGGGGGGGLGSGGSIR.S	2
*	Mif2_02_itms.08235.08235.2	4.2356	0.4811	100.0%	2706.7722	2706.7605	1	8.259	37.1%	8	R.GGGGSFGYSYGGGSGGGFSASSLGGGFGGGSR.G	2
*	Mif2_03_itms.04530.04530.2	3.9099	0.2984	100.0%	1792.5521	1792.7324	1	7.501	50.0%	8	R.GGSGGSYGGGGSGGGYGGGSGSR.G	2

contaminant_KERATIN03

9.1%

593

59519

5.2

no description

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	Mif2_02_itms.06539.06539.3	3.7285	0.2712	95.0%	2562.2344	2560.6995	1	4.234	29.0%	1	S.LRISSSKGS*LGGGFSSGGFSGGSFSR.G	3
*	Mif2_02_itms.05636.05636.2	3.2695	0.3775	100.0%	2098.1921	2098.193	1	7.648	47.7%	2	I.SSSKGSLGGGFSSGGFSGGSFSR.G	2
*	Mif2_02_itms.06546.06546.2	5.4195	0.3934	100.0%	1707.9321	1708.7844	1	7.622	72.2%	17	K.GSLGGGFSSGGFSGGSFSR.G	2
*	Mif2_02_itms.05549.05549.2	2.8067	0.2584	100.0%	1451.5521	1451.4949	3	6.165	50.0%	3	L.GGGFSSGGFSGGSFSR.G	2
*	Mif2_02_itms.04973.04973.2	2.9401	0.187	96.3%	1382.4922	1382.4668	4	4.685	68.2%	1	R.ALEESNYELEGK.I	2
*	Mif2_02_itms.11610.11610.2	3.4397	0.1599	95.1%	1797.4521	1798.0898	3	4.716	53.3%	1	R.NVQALEIELQSQLALK.Q	2

contaminant_KERATIN21

8.1%

357

39219

5.2

no description

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
	Mif2_03_itms.11680.11680.2	4.071	0.1456	98.9%	1330.4122	1330.5211	1	5.495	77.3%	3	R.NLDLDSIIAEVK.A	2 2 2 2
*	Mif2_02_itms.05078.05078.2	3.8783	0.364	100.0%	1448.4321	1448.6163	5	6.037	53.1%	2	R.AIGGGLSSVGGGSSTIK.Y	2

Similarities: contaminant_KERATIN22(1:1)
contaminant_KERATIN17(1:1)
contaminant_KERATIN18(1:1)

contaminant_KERATIN22

7.4%

645

65865

8.0

no description

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	Mif2_02_itms.04702.04702.2	3.1672	0.4315	100.0%	1255.5721	1255.3298	1	6.898	65.4%	1	R.GFSSGSAVVSGGSR.R	2
*	Mif2_02_itms.07323.07323.2	3.9275	0.4875	100.0%	1840.3922	1840.0055	1	7.712	45.2%	2	K.SISISVAGGGGGFGAAGGFGGR.G	2
	Mif2_03_itms.11680.11680.2	4.071	0.1456	98.9%	1330.4122	1330.5211	1	5.495	77.3%	3	R.NLDLDSIIAEVK.A	2 2 2 2

Similarities: contaminant_KERATIN21(1:2)
contaminant_KERATIN17(1:2)
contaminant_KERATIN18(1:2)

contaminant_KERATIN13

6.4%

643

65494

6.6

no description

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	Mif2_02_itms.05217.05217.2	3.4677	0.1898	100.0%	1181.2122	1180.303	1	5.615	88.9%	4	K.YEELQITAGR.H	2
*	Mif2_02_itms.04580.04580.2	4.2982	0.3489	100.0%	2385.8123	2385.298	1	8.788	36.7%	3	R.GGGGGGYGSGGSSYGSGGGSYGSGGGGGGGR.G	2

contaminant_gi|67568|pir||ELPG

5.6%

266

28821

8.1

pancreatic elastase (EC 3.4.21.36) I precursor - pig

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	Mif2_02_itms.14451.14451.2	2.9041	0.3781	100.0%	1450.4521	1450.6775	5	6.403	58.3%	1	R.VSAYISWINNVIA.S	2
*	Mif2_02_itms.14610.14610.2	3.1043	0.3338	100.0%	1537.2522	1537.7557	1	6.844	53.8%	3	R.VSAYISWINNVIAS.N	2
*	Mif2_02_itms.14589.14589.2	4.0163	0.2256	100.0%	1654.6322	1651.8595	1	5.044	71.4%	6	R.VSAYISWINNVIASN.-	2

contaminant_KERATIN17

4.4%

590

62461

8.1

no description

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	Mif2_02_itms.05182.05182.2	3.5665	0.3369	100.0%	1410.8722	1411.5547	1	6.573	73.1%	4	R.SFSTASAITPSVSR.T	2
	Mif2_03_itms.11680.11680.2	4.071	0.1456	98.9%	1330.4122	1330.5211	1	5.495	77.3%	3	R.NLDLDSIIAEVK.A	2 2 2 2

Similarities: contaminant_KERATIN21(1:1)
contaminant_KERATIN22(1:1)
contaminant_KERATIN18(1:1)

contaminant_KERATIN18

3.9%

562

59822

8.0

no description

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	Mif2_02_itms.05072.05072.2	3.4243	0.4159	100.0%	1027.4922	1027.1222	1	7.168	88.9%	4	R.SGFSSISVSR.S	2
	Mif2_03_itms.11680.11680.2	4.071	0.1456	98.9%	1330.4122	1330.5211	1	5.495	77.3%	3	R.NLDLDSIIAEVK.A	2 2 2 2

Similarities: contaminant_KERATIN21(1:1)
contaminant_KERATIN22(1:1)
contaminant_KERATIN17(1:1)

	Proteins	Peptide IDs	Spectra
Unfiltered	10709	67274	75550
Filtered	9	27	197
Forward matches	9	27	197
Decoy matches	0	0	0
Forward FP rate	0.0%	0.0%	0.0%

/data/1/catclw/Projects/Jonathan/step_2-5