D SPB

DTASelect v2.0.3
/data/1/catclw/Projects/SPB/SPB
/scratch/yates/SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta
SEQUEST 3.0 in SQT format.
--modstat -p 1
Jump to the summary table.

sequest.params modifications:

*	STY	80.0
#	X	0.0
@	X	0.0
Static	C	57.0

true	Use criteria
0.0	Minimum peptide confidence
0.05	Peptide false positive rate
0.0	Minimum protein confidence
1.0	Protein false positive rate
1	Minimum charge state
9	Maximum charge state
0.0	Minimum ion proportion
1000	Maximum Sp rank
-1.0	Minimum Sp score
Include	Modified peptide inclusion
Any	Tryptic status requirement
true	Multiple, ambiguous IDs allowed
Ignore	Peptide validation handling
XCorr	Purge duplicate peptides by protein
false	Include only loci with unique peptide
true	Remove subset proteins
Ignore	Locus validation handling
0	Minimum modified peptides per locus
1000	Minimum redundancy for low coverage loci
1	Minimum peptides per locus

Locus Key:

Validation Status

Locus

Sequence Count

Spectrum Count

Sequence Coverage

Length

MolWt

Descriptive Name

Similarity Key:

Locus

# of identical peptides

# of differing peptides

YIL002W-A

79.7%

7729

4.7

YIL002W-A SGDID:S000028835, Chr IX from 350298-350507, Uncharacterized ORF, "Identified by expression profiling and mass spectrometry"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	08312006.01278.01278.2	3.0438	0.4431	100.0%	1190.8121	1191.237	1	7.88	72.7%	1	R.DTPEDVSTAGAK.D	2
*	08312006.01282.01282.1	2.2445	0.3343	100.0%	1191.45	1191.237	2	6.707	45.5%	1	R.DTPEDVSTAGAK.D	1
*	08312006.04104.04104.3	2.5866	0.2841	100.0%	2328.2944	2328.622	1	5.177	34.5%	1	R.DTPEDVSTAGAKDILDVLNLLK.G	3
*	08312006.04092.04092.2	4.5461	0.5021	100.0%	2328.7922	2328.622	1	9.051	40.5%	1	R.DTPEDVSTAGAKDILDVLNLLK.G	2
*	08312006.03946.03946.2	3.5856	0.3306	100.0%	1156.2722	1156.4081	2	7.088	77.8%	1	K.DILDVLNLLK.G	2
*	08312006.03948.03948.1	3.3118	0.3593	100.0%	1157.71	1156.4081	1	6.697	72.2%	1	K.DILDVLNLLK.G	1
*	08312006.02335.02335.2	3.1414	0.3665	100.0%	1564.9922	1563.8036	1	5.895	58.3%	1	K.ISEVELKLDEMEK.K	2
*	08312006.02928.02928.3	3.2744	0.3498	100.0%	2341.7944	2340.6135	1	6.158	31.6%	1	K.MDSLLVQLEDLHRDNNDLAK.S	3

YBR109C

55.1%

147

16135

4.3

CMD1 SGDID:S000000313, Chr II from 458356-457913, reverse complement, Verified ORF, "Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	08312006.03063.03063.3	5.3458	0.479	100.0%	2602.9143	2603.8633	1	8.723	31.5%	1	K.EAFALFDKDNNGSISSSELATVMR.S	3
*	08312006.04306.04306.3	4.9941	0.4949	100.0%	4110.474	4109.526	1	6.668	23.6%	2	R.SLGLSPSEAEVNDLMNEIDVDGNHQIEFSEFLALMSR.Q	3
*	08312006.03518.03518.2	5.4312	0.6187	100.0%	2107.632	2107.325	1	11.144	52.6%	1	R.EVSDGSGEINIQQFAALLSK.-	2

YKL042W

46.0%

363

42271

7.9

SPC42 SGDID:S000001525, Chr XI from 358119-359210, Verified ORF, "Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	08312006.02802.02802.2	4.0157	0.4633	100.0%	2607.4722	2608.7546	1	7.718	50.0%	1	R.LYDDYYNIPYQYSNPT*PMNR.D	2
*	08312006.01202.01202.1	1.6637	0.2134	100.0%	925.43	925.93036	15	4.618	64.3%	1	R.DYNDVGSR.I	1
*	08312006.02439.02439.2	3.9558	0.3677	100.0%	2597.7922	2598.7546	1	6.917	42.9%	1	K.VKDPMVDDDPVS*ENYDQINVPK.H	2
*	08312006.02442.02442.3	3.5477	0.3094	100.0%	2598.6243	2598.7546	1	5.672	34.5%	1	K.VKDPMVDDDPVS*ENYDQINVPK.H	3
*	08312006.03162.03162.3	4.0244	0.3706	100.0%	2845.5544	2846.058	1	6.206	25.0%	1	R.SEDGNNDRMS*PLPSPLNTILPINNR.L	3
*	08312006.03367.03367.3	3.7286	0.3729	100.0%	2925.5645	2926.058	1	5.87	26.0%	1	R.SEDGNNDRMSPLPSPLNTILPINNR.L	3
*	08312006.02644.02644.2	3.7914	0.443	100.0%	1958.1322	1958.1624	1	7.965	50.0%	1	K.VNPSDDDIMMYESAELK.R	2
*	08312006.01892.01892.2	3.0533	0.1857	98.5%	1275.7122	1274.4136	1	5.188	77.8%	1	K.RVEEEIEELK.R	2
*	08312006.03438.03438.3	4.696	0.4771	100.0%	3063.2043	3064.4097	1	7.262	27.9%	1	K.LSLNNQLQELQSMMDGDDNIKLDNVSK.H	3
*	08312006.02158.02158.2	4.4024	0.6429	100.0%	2255.0723	2254.2546	1	10.727	55.6%	1	R.DYSPSSDACLECSNDLYEK.N	2
*	08312006.01880.01880.2	2.5977	0.3831	100.0%	2227.9521	2228.355	1	5.543	41.7%	1	R.VKPENNMSETFATPT*PNNR.-	2
*	08312006.01831.01831.2	2.7319	0.2745	97.0%	2228.372	2228.355	1	5.07	44.4%	2	R.VKPENNMSETFAT*PTPNNR.-	2
*	08312006.01795.01795.3	4.1287	0.4226	100.0%	2230.1042	2228.355	1	6.198	36.1%	1	R.VKPENNMSETFAT*PTPNNR.-	3
*	08312006.01842.01842.2	2.8391	0.2192	95.0%	2307.9922	2308.355	1	5.384	38.9%	1	R.VKPENNMSETFATPTPNNR.-	2

contaminant_SPA1_STAAU

31.1%

524

57320

5.7

owl|P02976| IMMUNOGLOBULIN G BINDING PROTEIN A PRECURSOR (PROTEIN A). - STAPHYLOCOCCUS...

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	08312006.03242.03242.3	3.1723	0.1819	100.0%	3514.0444	3513.7795	1	4.524	21.6%	2	K.ADAQQNNFNKDQQSAFYEILNMPNLNEAQR.N	3
*	08312006.03392.03392.2	2.9335	0.4456	100.0%	2382.0522	2382.6099	1	6.835	42.1%	1	K.DQQSAFYEILNMPNLNEAQR.N	2
	08312006.02612.02612.2	4.1759	0.4416	100.0%	2349.172	2349.5596	1	6.823	45.2%	2	R.NGFIQSLKDDPSQSTNVLGEAK.K	2
	08312006.02614.02614.3	2.8454	0.1447	100.0%	2350.4944	2349.5596	1	4.056	34.5%	1	R.NGFIQSLKDDPSQSTNVLGEAK.K	3
	08312006.01694.01694.2	4.1838	0.5628	100.0%	1460.2922	1461.5254	1	9.848	73.1%	2	K.DDPSQSTNVLGEAK.K	2
	08312006.03262.03262.3	5.0974	0.3424	100.0%	3285.7444	3285.5286	1	6.884	32.7%	1	K.ADNNFNKEQQNAFYEILNMPNLNEEQR.N	3
	08312006.03318.03318.3	4.1999	0.3224	100.0%	2481.1443	2481.699	1	6.293	38.2%	1	K.EQQNAFYEILNMPNLNEEQR.N	3
	08312006.03312.03312.2	4.1212	0.4949	100.0%	2481.392	2481.699	1	9.143	52.6%	1	K.EQQNAFYEILNMPNLNEEQR.N	2
*	08312006.02670.02670.2	3.5743	0.5046	100.0%	2363.152	2363.5864	1	7.484	40.5%	2	R.NGFIQSLKDDPSQSANLLSEAK.K	2
*	08312006.02828.02828.3	4.4973	0.4626	100.0%	2364.1743	2363.5864	1	6.702	28.6%	2	R.NGFIQSLKDDPSQSANLLSEAK.K	3
*	08312006.01978.01978.2	3.9423	0.4727	100.0%	1475.4122	1475.5522	1	9.359	76.9%	1	K.DDPSQSANLLSEAK.K	2
	08312006.03284.03284.3	3.2964	0.4167	100.0%	2485.8843	2486.7031	1	6.124	30.3%	1	K.EQQNAFYEILHLPNLNEEQR.N	3
	08312006.03286.03286.2	3.4099	0.333	100.0%	2487.2122	2486.7031	1	6.162	42.1%	1	K.EQQNAFYEILHLPNLNEEQR.N	2
	08312006.02866.02866.2	3.554	0.3693	100.0%	2347.632	2347.5872	1	6.345	45.2%	2	R.NGFIQSLKDDPSQSANLLAEAK.K	2
	08312006.02715.02715.3	4.695	0.2368	100.0%	2348.0344	2347.5872	1	6.855	41.7%	2	R.NGFIQSLKDDPSQSANLLAEAK.K	3
	08312006.02026.02026.2	4.0318	0.5305	100.0%	1458.9722	1459.5529	1	9.672	76.9%	1	K.DDPSQSANLLAEAK.K	2
	08312006.03360.03360.2	3.408	0.3659	100.0%	2473.5923	2473.7043	1	6.655	36.8%	1	K.EQQNAFYEILHLPNLTEEQR.N	2
	08312006.03354.03354.3	2.7746	0.2621	100.0%	2476.3743	2473.7043	1	4.885	27.6%	1	K.EQQNAFYEILHLPNLTEEQR.N	3

YNL225C

23.4%

581

67400

6.0

CNM67 SGDID:S000005169, Chr XIV from 224470-222725, reverse complement, Verified ORF, "Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	08312006.02662.02662.3	4.3243	0.3526	100.0%	3296.6042	3297.4758	4	6.636	22.3%	2	R.LDSPVS*ENGEIKDGEPIPQNWLNENHVGK.S	3
*	08312006.02742.02742.3	3.9193	0.3663	100.0%	3376.0745	3377.4758	2	6.126	23.2%	1	R.LDSPVSENGEIKDGEPIPQNWLNENHVGK.S	3
*	08312006.01878.01878.2	3.0429	0.4441	100.0%	2246.4922	2246.2983	1	6.483	38.2%	1	R.DSYEENKS*PSMDQMNYAR.N	2
*	08312006.01510.01510.2	2.5998	0.3535	100.0%	1814.3922	1814.8632	1	6.767	57.1%	1	R.NTSYQES*PGLQERPK.N	2
*	08312006.01174.01174.2	2.2855	0.2758	97.2%	1276.6322	1277.3335	41	4.586	50.0%	1	K.DKS*PIGTDVHK.K	2
*	08312006.02054.02054.2	2.0883	0.3061	97.1%	1363.0721	1363.4294	1	5.183	65.0%	1	K.DVPNFIHST*PR.E	2
*	08312006.01832.01832.3	4.4041	0.47	100.0%	2967.5044	2966.9226	1	6.488	30.8%	1	R.ANEQASAQPTDEHTSPDIS*IEDCNGAK.I	3
*	08312006.04506.04506.3	6.6609	0.622	100.0%	2907.2344	2906.2585	1	10.536	36.5%	1	R.KAELFEIPIGILFFDLYDSEENSSK.L	3
*	08312006.04658.04658.2	4.6888	0.6253	100.0%	2776.132	2778.0845	1	12.346	50.0%	1	K.AELFEIPIGILFFDLYDSEENSSK.L	2

YOR257W

16.8%

161

18751

4.6

CDC31 SGDID:S000005783, Chr XV from 811006-811491, Verified ORF, "Component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; interacts with Kar1p"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	08312006.02916.02916.2	3.9532	0.4846	100.0%	1823.6322	1823.9542	1	8.666	67.9%	1	K.REILDLIDEYDSEGR.H	2
*	08312006.03074.03074.2	4.5297	0.5585	100.0%	1667.4122	1667.7667	1	8.906	65.4%	1	R.EILDLIDEYDSEGR.H	2
*	08312006.02179.02179.2	3.2117	0.3712	100.0%	1404.9722	1405.5016	1	7.02	72.7%	1	K.ELGETLTDEELR.A	2

YIL149C

16.4%

1679

195140

6.0

MLP2 SGDID:S000001411, Chr IX from 68067-63028, reverse complement, Verified ORF, "Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved in the Tel1p pathway that controls telomere length"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	08312006.03647.03647.2	4.2002	0.5261	100.0%	2409.7522	2409.7876	1	8.996	47.5%	1	K.ISEFLNVPFESLQGVTYPVLR.K	2
*	08312006.01958.01958.2	3.5522	0.486	100.0%	1687.5521	1687.804	1	8.116	57.7%	1	K.EELNGLKDQLNEER.S	2
*	08312006.01570.01570.2	4.0373	0.4369	100.0%	1447.6721	1448.443	1	7.827	75.0%	1	R.DQGNDSLNDDLNK.E	2
*	08312006.01526.01526.2	4.4904	0.4726	100.0%	1818.9922	1819.8363	1	8.606	63.3%	4	R.DQGNDSLNDDLNKENK.L	2
*	08312006.02591.02591.2	2.8155	0.3628	100.0%	2601.2722	2601.666	22	5.386	26.2%	1	K.NDDNSCRNPEHTDVIDELIDTK.L	2
*	08312006.02587.02587.3	5.2837	0.5835	100.0%	2601.5942	2601.666	1	9.512	35.7%	1	K.NDDNSCRNPEHTDVIDELIDTK.L	3
*	08312006.02587.02587.2	2.2227	0.2969	98.6%	1734.7322	1739.8767	1	5.218	42.9%	1	R.NPEHTDVIDELIDTK.L	3
*	08312006.04026.04026.3	2.97	0.2778	100.0%	2144.3943	2145.4197	2	4.74	39.1%	1	K.FQLQNQLEDFILELEHK.T	3
*	08312006.02378.02378.2	5.3893	0.5158	100.0%	2245.7722	2246.4363	1	10.612	63.2%	2	K.ILESSNIVNENDSQAIITER.L	2
*	08312006.01567.01567.2	2.3799	0.3124	98.6%	1882.6921	1882.9966	1	6.732	43.3%	1	K.ESEISHNENKMDFSSK.E	2
*	08312006.03722.03722.2	5.5297	0.497	100.0%	2692.6921	2693.9243	1	9.513	50.0%	5	K.DANSQIQAYEEIISSNENALIELK.N	2
*	08312006.03723.03723.3	5.6026	0.3433	100.0%	2693.5745	2693.9243	1	6.836	33.7%	2	K.DANSQIQAYEEIISSNENALIELK.N	3
*	08312006.01378.01378.2	3.9878	0.5663	100.0%	1221.8121	1222.2604	1	9.201	80.0%	1	K.DAADCQAELTK.T	2
*	08312006.01130.01130.2	3.0995	0.3769	100.0%	1613.5122	1613.6812	1	7.671	53.6%	1	K.VDDTAANNGDKDHLK.L	2
*	08312006.02168.02168.2	2.6602	0.1842	98.6%	1530.2922	1529.7114	1	5.512	72.7%	1	K.ILVYESEMEQCK.Q	2
*	08312006.01966.01966.3	3.0812	0.1492	100.0%	1587.9844	1587.7294	65	4.268	37.5%	1	K.DKLEQDLHFENAK.V	3
*	08312006.01962.01962.2	4.8263	0.387	100.0%	1590.8922	1587.7294	1	7.885	58.3%	2	K.DKLEQDLHFENAK.V	2
*	08312006.02138.02138.2	4.7705	0.5486	100.0%	1735.2122	1735.7582	1	9.539	73.3%	1	K.GSGEDAEEELWNSPSK.G	2
*	08312006.02150.02150.2	4.3785	0.4627	100.0%	1814.7122	1815.7582	2	8.259	66.7%	1	K.GSGEDAEEELWNSPS*K.G	2
*	08312006.02194.02194.2	3.7811	0.4499	100.0%	1815.5721	1815.7582	1	7.099	53.3%	1	K.GSGEDAEEELWNS*PSK.G	2
*	08312006.02607.02607.3	4.2403	0.3694	100.0%	3470.9944	3472.5767	1	6.027	25.8%	1	K.GSGEDAEEELWNS*PSKGNSERPSAVAGFINQK.N	3
*	08312006.02703.02703.3	3.2649	0.3302	100.0%	3475.4944	3472.5767	1	4.432	21.0%	1	K.GSGEDAEEELWNSPS*KGNSERPSAVAGFINQK.N	3
*	08312006.01882.01882.2	4.3574	0.5856	100.0%	1686.2522	1686.7906	1	10.361	57.1%	1	K.NDVSFNDSQSMVTNK.E	2
*	08312006.02175.02175.3	5.146	0.5382	100.0%	2986.5244	2987.1382	1	8.292	33.3%	1	K.NDVSFNDSQSMVTNKENNIVDSSAAGNK.A	3
*	08312006.01339.01339.2	2.472	0.3124	98.6%	1319.7922	1319.3708	4	6.12	62.5%	1	K.ENNIVDSSAAGNK.A	2
*	08312006.01082.01082.2	2.5214	0.3041	96.9%	1698.6322	1698.741	23	5.19	42.9%	1	K.RPIESGTSS*DPDTKK.V	2

YPL124W

16.2%

253

29280

9.5

SPC29 SGDID:S000006045, Chr XVI from 316754-317515, Verified ORF, "Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inner plaque component Spc110p; required for SPB duplication"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	08312006.01346.01346.2	2.2469	0.2557	97.4%	1217.2122	1217.2377	1	5.127	87.5%	1	K.KFQDDT*LNR.V	2
*	08312006.01723.01723.2	3.4635	0.298	100.0%	1906.3722	1905.9298	1	5.832	50.0%	1	K.LREENFSSNT*SELGNK.K	2
*	08312006.02206.02206.2	4.3106	0.4677	100.0%	1923.7322	1923.9855	1	8.365	63.3%	5	R.SSQIHIENEST*EDILK.I	2
*	08312006.02134.02134.3	4.7146	0.5164	100.0%	1923.8644	1923.9855	1	7.705	50.0%	1	R.SSQIHIENEST*EDILK.I	3

YDR356W

15.4%

944

111782

7.1

SPC110 SGDID:S000002764, Chr IV from 1186098-1188932, Verified ORF, "Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	08312006.01710.01710.2	2.3958	0.3627	100.0%	1203.3722	1203.162	1	6.474	77.8%	1	R.S*IDDTIDSTR.L	2
*	08312006.02979.02979.2	3.2391	0.4192	100.0%	1940.4122	1941.0148	1	5.933	63.3%	1	R.LFSEASQFDDS*FPEIK.A	2
*	08312006.01286.01286.2	2.0552	0.3293	96.4%	931.5722	931.981	6	5.743	50.0%	3	K.ANIPPS*PR.S	2
*	08312006.02379.02379.2	4.6325	0.4422	100.0%	2401.1921	2400.4688	1	8.64	52.6%	1	K.EKNDTLNNYDTLEEETDDLK.N	2
*	08312006.02522.02522.2	4.5437	0.5893	100.0%	2142.3323	2143.1792	1	9.8	58.8%	1	K.NDTLNNYDTLEEETDDLK.N	2
*	08312006.02415.02415.2	3.8766	0.5835	100.0%	2411.7322	2413.4705	1	9.618	42.1%	1	K.NDTLNNYDTLEEETDDLKNR.L	2
*	08312006.02418.02418.3	3.5783	0.3389	100.0%	2412.4443	2413.4705	1	6.143	35.5%	1	K.NDTLNNYDTLEEETDDLKNR.L	3
*	08312006.02346.02346.2	5.4879	0.5878	100.0%	1831.3522	1831.9744	1	11.141	73.3%	2	K.DQVLELENNSDVQSLK.L	2
*	08312006.01614.01614.2	3.2758	0.2818	100.0%	1205.9922	1206.2921	1	6.273	83.3%	1	K.DSEDKIEELK.I	2
*	08312006.02994.02994.2	5.5709	0.4882	100.0%	2350.5723	2351.5786	1	9.419	57.9%	1	K.SEQSNIQHDLNLQILNLENK.L	2
*	08312006.01330.01330.2	3.3778	0.3401	100.0%	1589.1522	1589.6592	1	7.303	62.5%	1	R.EKEELNENSNNIR.I	2
*	08312006.01334.01334.3	2.6713	0.2498	100.0%	1589.6643	1589.6592	11	4.277	35.4%	1	R.EKEELNENSNNIR.I	3
*	08312006.02763.02763.2	3.6607	0.4648	100.0%	1696.9321	1696.8113	1	7.769	57.7%	1	K.NYLSEITSLQEENR.R	2
*	08312006.03075.03075.2	4.711	0.5972	100.0%	1920.4122	1921.0858	1	10.386	70.0%	1	K.DNDSTMQLNDIISYYK.L	2

YOR373W

14.6%

851

94104

7.0

NUD1 SGDID:S000005900, Chr XV from 1036830-1039385, Verified ORF, "Component of the spindle pole body outer plaque, required for exit from mitosis"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	08312006.01968.01968.2	2.2053	0.3403	96.7%	2101.1921	2100.1655	15	5.61	30.6%	1	K.VPSGFSGTTATS*HQEAQWK.Q	2
*	08312006.03375.03375.3	3.5934	0.4393	100.0%	3634.0444	3633.9507	1	5.851	21.4%	1	K.QYFPGIGS*GGGTNFGGAVGTANKVPESDLIVSDLVK.D	3
*	08312006.01306.01306.2	5.017	0.4941	100.0%	2171.3323	2172.152	1	8.994	61.1%	3	K.NNNNNNNNNNNNSININNK.D	2
*	08312006.01314.01314.3	3.9879	0.3812	100.0%	2172.1143	2172.152	1	6.812	40.3%	1	K.NNNNNNNNNNNNSININNK.D	3
*	08312006.01290.01290.3	3.9957	0.3066	100.0%	2627.8145	2628.6106	1	5.699	33.0%	1	K.NNNNNNNNNNNNSININNKDNAR.T	3
*	08312006.01896.01896.2	3.5537	0.4718	100.0%	2337.2722	2338.401	1	6.96	39.5%	1	K.KIQEEENLANSDDT*PLDTPK.F	2
*	08312006.02046.02046.2	6.0849	0.6108	100.0%	2129.4922	2130.2268	1	10.882	66.7%	1	K.IQEEENLANSDDTPLDTPK.F	2
*	08312006.02052.02052.2	4.1295	0.3313	100.0%	2209.5322	2210.2268	1	8.115	50.0%	1	K.IQEEENLANSDDTPLDT*PK.F	2
*	08312006.02090.02090.2	4.0379	0.4012	100.0%	2210.632	2210.2268	1	6.971	52.8%	1	K.IQEEENLANS*DDTPLDTPK.F	2
*	08312006.01982.01982.2	4.6245	0.33	100.0%	2211.3123	2210.2268	1	7.868	58.3%	1	K.IQEEENLANSDDT*PLDTPK.F	2
*	08312006.02955.02955.3	4.4004	0.3909	100.0%	3075.8342	3076.211	1	5.437	30.0%	1	K.IQEEENLANSDDTPLDT*PKFNDLFTK.N	3
*	08312006.02471.02471.2	4.9599	0.5511	100.0%	2142.5122	2143.2659	1	10.21	58.3%	1	R.VEDITSISEVNTSFNETEK.Q	2
*	08312006.02458.02458.2	3.745	0.4366	100.0%	2222.892	2223.2659	1	8.325	50.0%	1	R.VEDITSISEVNTS*FNETEK.Q	2

YPL255W

14.0%

385

45384

6.5

BBP1 SGDID:S000006176, Chr XVI from 67725-68882, Verified ORF, "Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periphery; forms a complex with a nuclear envelope protein Mps2p and SPB components Spc29p and Kar1p; required for mitotic functions of Cdc5p"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	08312006.02740.02740.2	3.6999	0.4814	100.0%	1462.8922	1462.5714	1	7.364	70.8%	1	K.DALFGTDIS*PSMK.Y	2
*	08312006.02230.02230.2	2.6208	0.3724	100.0%	1540.2922	1540.5461	1	6.218	58.3%	1	R.SNS*WSGLDSTLHR.K	2
*	08312006.01823.01823.2	1.882	0.362	96.3%	1273.5721	1273.4348	1	5.74	55.0%	1	R.SLRS*PAPIVPR.E	2
*	08312006.03826.03826.2	5.3451	0.5249	100.0%	2059.372	2059.2834	1	8.877	65.6%	1	R.DNYESEIHDLLLQLSLR.N	2

YJR053W

10.6%

574

66087

5.9

BFA1 SGDID:S000003814, Chr X from 533941-535665, Verified ORF, "Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pathways that control exit from mitosis"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	08312006.03190.03190.3	4.6334	0.4912	100.0%	3179.4844	3180.2068	1	7.049	28.0%	1	K.QADDDQDMEVDQDDEFLNDFQEFQNK.K	3
*	08312006.02300.02300.2	2.0528	0.3385	96.8%	1848.5521	1848.8773	8	5.461	38.5%	1	R.DWNTQQELDS*FKEK.R	2
*	08312006.02944.02944.2	5.1722	0.7149	100.0%	2355.3123	2356.5146	1	12.104	60.0%	1	R.DETIISVDEETIMDESTVNSK.R	2

YFR028C

9.8%

551

61907

8.0

CDC14 SGDID:S000001924, Chr VI from 210056-208401, reverse complement, Verified ORF, "Protein phosphatase required for mitotic exit; located in the nucleolus until liberated by the FEAR and Mitotic Exit Network in anaphase, enabling it to act on key substrates to effect a decrease in CDK/B-cyclin activity and mitotic exit"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	08312006.03530.03530.3	5.6056	0.4052	100.0%	3250.9744	3251.5354	1	7.366	29.6%	1	K.YEHVEFGDFNVLTPDFIAFASPQEDHPK.G	3
*	08312006.01699.01699.3	4.9983	0.4373	100.0%	2992.4944	2991.04	1	7.163	31.0%	1	R.KVVIESNNS*DDESMQDTNGTSNHYPK.V	3
*	08312006.01778.01778.3	3.1114	0.3301	100.0%	2861.5444	2862.866	1	5.507	29.2%	1	K.VVIESNNS*DDESMQDTNGTSNHYPK.V	3

YPL131W

9.4%

297

33715

6.8

RPL5 SGDID:S000006052, Chr XVI from 303120-304013, Verified ORF, "Protein component of the large (60S) ribosomal subunit with similarity to E. coli L18 and rat L5 ribosomal proteins; binds 5S rRNA and is required for 60S subunit assembly"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	08312006.04402.04402.3	4.2275	0.3177	100.0%	3343.5842	3343.602	1	5.565	22.2%	1	R.SYIFGGHVSQYMEELADDDEERFSELFK.G	3

YIR017C

9.1%

187

21590

10.1

MET28 SGDID:S000001456, Chr IX from 384116-383553, reverse complement, Verified ORF, "Transcriptional activator in the Cbf1p-Met4p-Met28p complex, participates in the regulation of sulfur metabolism"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	08312006.02047.02047.2	2.792	0.0748	97.5%	2064.2522	2064.324	190	3.627	37.5%	1	K.NFENMNKLQNLNTQINK.L	2

contaminant_KERATIN02

8.7%

622

61987

5.2

no description

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	08312006.03610.03610.3	3.7466	0.3181	100.0%	2906.3643	2904.1597	1	6.436	21.9%	1	K.NYSPYYNTIDDLKDQIVDLTVGNNK.T	3
*	08312006.03216.03216.3	4.2808	0.4373	100.0%	3265.3145	3266.413	1	7.627	28.6%	1	K.DIENQYETQITQIEHEVSSSGQEVQSSAK.E	3

YNL178W

7.1%

240

26503

9.4

RPS3 SGDID:S000005122, Chr XIV from 302682-303404, Verified ORF, "Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; has similarity to E. coli S3 and rat S3 ribosomal proteins"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	08312006.01854.01854.2	3.2958	0.3914	100.0%	1904.9521	1905.9696	1	7.985	53.1%	1	K.DYRPAEETEAQAEPVEA.-	2

U	YDL229W	2	2	6.9%	613	66602	5.4	SSB1 SGDID:S000002388, Chr IV from 44066-45907, Verified ORF, "Cytoplasmic ATPase that is a ribosome-associated molecular chaperone; may be involved in the folding of newly-synthesized polypeptide chains; member of the heat shock protein 70 (HSP70) family; interacts with the phosphatase subunit Reg1p"
U	YNL209W	2	2	6.9%	613	66595	5.5	SSB2 SGDID:S000005153, Chr XIV from 252060-253901, Verified ORF, "Cytoplasmic ATPase that is a ribosome-associated molecular chaperone; may be involved in the folding of newly-synthesized polypeptide chains; member of the heat shock protein 70 (HSP70) family; homolog of SSB1"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
	08312006.03100.03100.2	4.1257	0.6	100.0%	2160.5322	2161.4138	1	9.624	55.6%	1	K.VIDVDGNPVIEVQYLEETK.T	2
	08312006.03572.03572.2	3.4499	0.4365	100.0%	2413.7522	2412.6562	1	8.143	36.4%	1	K.IEAALSDALAALQIEDPSADELR.K	2

YCR015C

6.9%

317

36278

7.4

YCR015C SGDID:S000000608, Chr III from 142167-141214, reverse complement, Uncharacterized ORF, "Hypothetical protein"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	08312006.02688.02688.2	1.9161	0.3999	100.0%	2772.872	2766.853	1	5.862	26.2%	1	K.TIIISDFDETITRVDTICT*IAK.L	2

U	YAL005C	2	2	6.5%	642	69768	5.1	SSA1 SGDID:S000000004, Chr I from 141433-139505, reverse complement, Verified ORF, "ATPase involved in protein folding and nuclear localization signal (NLS)-directed nuclear transport; member of heat shock protein 70 (HSP70) family; forms a chaperone complex with Ydj1p; localized to the nucleus, cytoplasm, and cell wall"
U	YLL024C	2	2	6.6%	639	69470	5.1	SSA2 SGDID:S000003947, Chr XII from 97484-95565, reverse complement, Verified ORF, "ATP binding protein involved in protein folding and vacuolar import of proteins; member of heat shock protein 70 (HSP70) family; associated with the chaperonin-containing T-complex; present in the cytoplasm, vacuolar membrane and cell wall"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
	08312006.01667.01667.2	3.7467	0.4987	100.0%	1676.2522	1676.6964	1	8.413	50.0%	1	K.ATAGDTHLGGEDFDNR.L	2
	08312006.03443.03443.2	3.0807	0.3051	98.5%	2583.2122	2578.7925	1	6.209	26.0%	1	R.SINPDEAVAYGAAVQAAILTGDESSK.T	2

YMR131C

6.3%

511

57261

4.6

RRB1 SGDID:S000004738, Chr XIII from 534697-533162, reverse complement, Verified ORF, "Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with the ribosomal protein Rpl3p"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	08312006.02639.02639.3	3.5821	0.305	100.0%	3701.2444	3701.7546	1	4.914	20.2%	1	K.TLLKDDNEGEDDEEDDEDDVDPVIENENIPLR.D	3

YMR024W

6.2%

390

44000

9.4

MRPL3 SGDID:S000004626, Chr XIII from 321874-323046, Verified ORF, "Mitochondrial ribosomal protein of the large subunit"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	08312006.03894.03894.2	2.4923	0.3363	96.0%	2781.2722	2780.832	1	5.458	30.4%	1	K.SPVFIVHVFSGEETLGEGYGSS*LK.E	2

YDR171W

6.1%

375

42817

5.1

HSP42 SGDID:S000002578, Chr IV from 806616-807743, Verified ORF, "Small cytosolic stress-induced chaperone that forms barrel-shaped oligomers and suppresses the aggregation of non-native proteins; oligomer dissociation is not required for function; involved in cytoskeleton reorganization after heat shock"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	08312006.03088.03088.2	4.2448	0.5294	100.0%	2670.652	2671.828	1	8.405	38.6%	1	R.IAIEEIPDEELEFEENPNPTVEN.-	2

YDR432W

5.3%

414

45407

5.5

NPL3 SGDID:S000002840, Chr IV from 1328773-1330017, Verified ORF, "RNA-binding protein that carries poly(A)+ mRNA from the nucleus into the cytoplasm; phosphorylation by Sky1p in the cytoplasm may promote release of mRNAs"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	08312006.03938.03938.2	5.0982	0.5533	100.0%	2439.6921	2438.6501	1	9.97	50.0%	1	R.DFDGTGALEFPSEEILVEALER.L	2

YKL081W

5.1%

412

46520

7.8

TEF4 SGDID:S000001564, Chr XI from 282535-282739,283066-284099, Verified ORF, "Translation elongation factor EF-1 gamma"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	08312006.03160.03160.2	2.1509	0.2443	97.4%	2435.5723	2437.5806	1	4.253	30.0%	1	R.GQDFAPAFDVAPDWESYEYTK.L	2

YKL054C

4.2%

738

83973

5.0

DEF1 SGDID:S000001537, Chr XI from 338397-336181, reverse complement, Verified ORF, "RNAPII degradation factor, forms a complex with Rad26p in chromatin, enables ubiquitination and proteolysis of RNAPII"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	08312006.01662.01662.3	5.1769	0.519	100.0%	3613.7043	3614.653	1	7.957	24.2%	1	K.NNYNYYQTQNGQEQQSPNQGVAQHSEDSQQK.Q	3
*	08312006.01708.01708.3	4.4186	0.3356	100.0%	3695.6042	3694.653	1	6.483	24.2%	1	K.NNYNYYQTQNGQEQQS*PNQGVAQHSEDSQQK.Q	3

YOL041C

3.1%

459

51942

9.4

NOP12 SGDID:S000005401, Chr XV from 252644-251265, reverse complement, Verified ORF, "Nucleolar protein, required for pre-25S rRNA processing; contains an RNA recognition motif (RRM) and has similarity to Nop13p, Nsr1p, and putative orthologs in Drosophila and S. pombe"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	08312006.01248.01248.2	2.7255	0.0955	97.4%	1448.2322	1448.6134	72	4.835	34.6%	1	K.TDETSVPLTSAAKK.V	2

YBR235W

2.9%

1120

123998

8.5

YBR235W SGDID:S000000439, Chr II from 686896-690258, Uncharacterized ORF, "Hypothetical protein"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	08312006.02492.02492.3	2.8761	0.1326	97.2%	3558.8342	3561.1155	35	3.687	19.4%	1	K.GDQPAAITTNFDTYTLILQLAAILVTVPEWKR.T	3

Reverse_YPL256C

2.9%

545

61697

5.8

CLN2 SGDID:S000006177, Chr XVI from 66614-64977, reverse complement, Verified ORF, "G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p)"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	08312006.02423.02423.2	2.6325	0.3341	95.8%	1968.1122	1967.8687	1	4.759	46.7%	1	R.EDVTADSDQSLQSQRK.E	2

YGL075C

2.8%

387

44585

8.3

MPS2 SGDID:S000003043, Chr VII from 368091-366928, reverse complement, Verified ORF, "Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required for insertion of the newly duplicated SPB into the nuclear envelope; potentially phosphorylated by Cdc28p"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	08312006.01310.01310.2	3.0261	0.4483	100.0%	1182.9521	1183.1771	1	7.53	70.0%	1	K.NTEGAGIST*PR.K	2

Reverse_YDR484W

2.3%

641

74333

5.8

VPS52 SGDID:S000002892, Chr IV from 1422753-1424678, Verified ORF, "Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	08312006.01254.01254.2	2.0502	0.2855	97.3%	1651.6921	1652.8163	1	5.168	42.9%	1	K.DFDKPALMASNEADK.N	2

YDR251W

2.2%

830

92872

8.4

PAM1 SGDID:S000002659, Chr IV from 960608-963100, Verified ORF, "Essential protein of unknown function; exhibits variable expression during colony morphogenesis; overexpression permits survival without protein phosphatase 2A, inhibits growth, and induces a filamentous phenotype"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	08312006.01911.01911.2	3.1801	0.0587	97.4%	2065.9722	2064.382	2	4.026	52.9%	2	K.MGIAMNNPNFQNEQTILK.H	2

Reverse_YKR031C

1.9%

1683

195203

7.6

SPO14 SGDID:S000001739, Chr XI from 506037-500986, reverse complement, Verified ORF, "Phospholipase D, catalyzes the hydrolysis of phosphatidylcholine, producing choline and phosphatidic acid; involved in Sec14p-independent secretion; required for meiosis and spore formation; differently regulated in secretion and meiosis"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	08312006.02619.02619.3	3.1016	0.0839	97.1%	3528.2644	3519.798	1	3.054	18.5%	1	K.ADDIEGSNFNTIKPIIQSLLDDLSGDDLEQNK.L	3

YMR205C

1.0%

959

104618

6.7

PFK2 SGDID:S000004818, Chr XIII from 674765-671886, reverse complement, Verified ORF, "Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	08312006.01847.01847.2	2.0287	0.3389	96.6%	1305.2722	1305.3464	1	5.182	66.7%	1	K.VHS*YTDLAYR.M	2

Reverse_YNL139C

0.9%

1597

183930

7.7

RLR1 SGDID:S000005083, Chr XIV from 365719-360926, reverse complement, Verified ORF, "Subunit of the THO complex, which is required for efficient transcription elongation and involved in transcriptional elongation-associated recombination; required for LacZ RNA expression from certain plasmids"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	08312006.01612.01612.2	2.983	0.314	98.5%	1703.8522	1703.8944	7	5.284	46.2%	1	K.VNDWISYFNVFGNK.L	2

YLR454W

0.6%

2628

303478

8.0

YLR454W SGDID:S000004446, Chr XII from 1043995-1051881, Uncharacterized ORF, "The authentic, non-tagged protein was localized to the mitochondria"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	08312006.04988.04988.2	1.7787	0.3328	97.3%	2278.172	2281.41	4	4.902	31.2%	1	K.HYLTRLCNPIYVQDLSK.H	2

Reverse_YDL140C

0.6%

1733

191610

5.6

RPO21 SGDID:S000002299, Chr IV from 210562-205361, reverse complement, Verified ORF, "RNA polymerase II largest subunit B220, part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	08312006.03256.03256.2	2.3493	0.0939	96.2%	1355.1522	1355.592	2	3.584	65.0%	1	R.MLENHEDLLLK.G	2

YLR024C

0.4%

1872

216777

6.3

UBR2 SGDID:S000004014, Chr XII from 193282-187664, reverse complement, Verified ORF, "Cytoplasmic ubiquitin-protein ligase (E3)"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	08312006.05747.05747.1	1.0164	0.3569	100.0%	830.31	829.9719	211	4.888	33.3%	1	K.LPTETIR.N	1

	Proteins	Peptide IDs	Spectra
Unfiltered	5185	7813	8980
Filtered	38	151	181
Forward matches	33	146	176
Decoy matches	5	5	5
Forward FP rate	15.15%	3.42%	2.84%

/data/1/catclw/Projects/SPB/SPB