D phospho2

359

39621

5.8

FBA1 SGDID:S000001543, Chr XI from 327131-326052, reverse complement, Verified ORF, "Fructose 1,6-bisphosphate aldolase, a cytosolic enzyme required for glycolysis and gluconeogenesis; catalyzes the conversion of fructose 1,6 bisphosphate into two 3-carbon products: glyceraldehyde-3-phosphate and dihydroxyacetone phosphate"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-06_itms_8.06634.06634.2	3.1677	0.371	100.0%	1399.7308	1400.5742	3	6.427	58.3%	2	K.TGVIVGEDVHNLF.T	2

YML110C

307

34685

6.6

COQ5 SGDID:S000004578, Chr XIII from 50954-50031, reverse complement, Verified ORF, "2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase, involved in ubiquinone (Coenzyme Q) biosynthesis; located in mitochondria"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-04_itms_8.06539.06539.2	2.302	0.1443	92.8%	1008.58777	1009.06055	406	4.227	60.0%	1	I.DVAGGSGDIAF.G	2

Reverse_YBR257W

279

32881

9.2

POP4 SGDID:S000000461, Chr II from 728880-729719, Verified ORF, "Subunit of both RNase MRP, which cleaves pre-rRNA, and nuclear RNase P, which cleaves tRNA precursors to generate mature 5' ends; binds to the RPR1 RNA subunit in Rnase P"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-03_itms_6.04747.04747.2	2.6355	0.1531	92.1%	1192.615	1193.4288	14	4.197	72.2%	1	D.TIKNEYALKL.C	2

YOL154W

249

27567

5.0

ZPS1 SGDID:S000005514, Chr XV from 34657-35406, Verified ORF, "Putative GPI-anchored protein; transcription is induced under low-zinc conditions, as mediated by the Zap1p transcription factor, and at alkaline pH"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-01_itms_15.09867.09867.2	1.4308	0.0695	92.9%	988.50995	986.1558	433	4.238	68.8%	1	K.FSSGKSIIF.A	2

YNR075W

3.5%

374

43867

7.5

COS10 SGDID:S000005358, Chr XIV from 779916-781040, Verified ORF, "Protein of unknown function, member of a family of conserved, often subtelomerically-encoded proteins"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-04_itms_5.05118.05118.2	1.0315	0.2448	99.4%	1518.7579	1517.6595	336	5.299	37.5%	1	P.DMSDFFICANLSP.A	2

YAL038W

3.4%

500

54545

7.7

CDC19 SGDID:S000000036, Chr I from 71787-73289, Verified ORF, "Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-05_itms_7.05903.05903.2	4.29	0.4025	100.0%	1724.9156	1725.9365	1	6.885	62.5%	3	K.GVNLPGTDVDLPALSEK.D	2

U	YML085C	1	2	3.4%	447	49800	5.1	TUB1 SGDID:S000004550, Chr XIII from 99400-99376,99259-97941, reverse complement, Verified ORF, "Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules"
U	YML124C	1	2	3.4%	445	49694	5.2	TUB3 SGDID:S000004593, Chr XIII from 23684-23660,23361-22049, reverse complement, Verified ORF, "Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
	02142006-Holinger-06_itms_9.06755.06755.2	4.3284	0.3282	100.0%	1744.9209	1745.9701	1	6.529	75.0%	2	R.AIYVDLEPNVIDEVR.N	2

YCR012W

3.4%

416

44738

7.6

PGK1 SGDID:S000000605, Chr III from 137743-138993, Verified ORF, "3-phosphoglycerate kinase, catalyzes transfer of high-energy phosphoryl groups from the acyl phosphate of 1,3-bisphosphoglycerate to ADP to produce ATP; key enzyme in glycolysis and gluconeogenesis"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-06_itms_9.06688.06688.2	2.9147	0.349	100.0%	1439.8271	1440.683	1	6.092	61.5%	1	K.ASAPGSVILLENLR.Y	2

YKL218C

3.4%

326

34899

7.2

SRY1 SGDID:S000001701, Chr XI from 18339-17359, reverse complement, Verified ORF, "3-hydroxyaspartate dehydratase, deaminates L-threo-3-hydroxyaspartate to form oxaloacetate and ammonia; required for survival in the presence of hydroxyaspartate"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-05_itms_8.06446.06446.2	2.1624	0.1216	90.8%	1138.5924	1139.3396	202	4.144	55.0%	1	Q.LAAEHGFALIP.P	2

YHR172W

3.3%

823

96825

6.4

SPC97 SGDID:S000001215, Chr VIII from 448335-450806, Verified ORF, "Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-05_itms_5.04787.04787.2	2.4953	0.1659	99.2%	1132.6415	1133.3329	85	5.135	55.6%	1	R.YTNNIPLLGK.L	2
*	02142006-Holinger-03_itms_6.05060.05060.2	4.2184	0.3186	100.0%	2090.9326	2092.2227	2	5.451	59.4%	1	R.YFNDYEPSDPETPIEFK.I	2

YOR360C

3.2%

526

61000

6.6

PDE2 SGDID:S000005887, Chr XV from 1014817-1013237, reverse complement, Verified ORF, "High-affinity cyclic AMP phosphodiesterase, component of the cAMP-dependent protein kinase signaling system, protects the cell from extracellular cAMP, contains readthrough motif surrounding termination codon"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-02_itms_12.08257.08257.2	2.3254	0.1992	99.1%	1790.9572	1793.931	1	4.84	50.0%	1	G.INKNSYGTVNGNSVPTQ.A	2

Reverse_YHL021C

3.2%

465

53135

7.5

YHL021C SGDID:S000001013, Chr VIII from 65856-64459, reverse complement, Uncharacterized ORF, "The authentic, non-tagged protein was localized to the mitochondria"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-06_itms_9.06705.06705.2	2.0425	0.2294	95.3%	1621.7864	1624.7068	102	4.34	42.9%	1	A.YHANVSTAQSANVDF.T	2

Reverse_YDR190C

3.2%

463

50453

5.9

RVB1 SGDID:S000002598, Chr IV from 841990-840599, reverse complement, Verified ORF, "Essential protein involved in transcription regulation; component of chromatin remodeling complexes; required for assembly and function of the INO80 complex; member of the RUVB-like protein family"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-04_itms_8.06473.06473.2	2.3104	0.1204	94.7%	1680.8379	1678.0281	1	4.315	50.0%	1	L.TRVILLRDILDPPVG.H	2

Reverse_YDR014W

3.1%

647

74722

5.9

RAD61 SGDID:S000002421, Chr IV from 474043-475986, Verified ORF, "Protein of unknown function; mutation confers radiation sensitivity"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-02_itms_8.06096.06096.2	4.1156	0.0908	91.7%	2304.0625	2305.7476	1	4.183	42.1%	2	H.VEMNTLLILIKILSDDDLMD.K	2

Reverse_YFR051C

3.1%

546

60628

5.2

RET2 SGDID:S000001947, Chr VI from 251790-250150, reverse complement, Verified ORF, "Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-06_itms_9.06736.06736.2	2.2896	0.2535	90.4%	1646.7799	1643.8553	210	4.13	37.5%	1	H.GARQSTAMPSAVPAVEA.A	2

Reverse_YJR002W

3.0%

593

66953

4.7

MPP10 SGDID:S000003762, Chr X from 438779-440560, Verified ORF, "Component of the SSU processome, which is required for pre-18S rRNA processing, interacts with and controls the stability of Imp3p and Imp4p, essential for viability; similar to human Mpp10p"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-05_itms_7.05716.05716.2	3.3401	0.0941	92.9%	2027.9716	2029.004	21	4.242	41.2%	1	A.STKEDDMSPEEDNESLSD.N	2

Reverse_YOR313C

3.0%

338

38591

9.6

SPS4 SGDID:S000005840, Chr XV from 902869-901853, reverse complement, Verified ORF, "Protein whose expression is induced during sporulation; not required for sporulation; heterologous expression in E. coli induces the SOS response that senses DNA damage"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-03_itms_9.06410.06410.2	2.8433	0.1848	94.3%	1113.7496	1112.4012	1	4.311	72.2%	2	T.QALIIRTLAI.K	2

Reverse_YLR114C

764

86426

4.5

YLR114C SGDID:S000004104, Chr XII from 377239-374945, reverse complement, Uncharacterized ORF, "Mutation is syntheticly lethal with apl2 vps1 double mutant"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-06_itms_20.13733.13733.2	1.4762	0.1504	94.1%	2612.152	2614.87	40	4.31	26.2%	1	D.YLLTFYTFTEEFSHSGDPLAQF.P	2

YBR158W

549

62686

9.1

AMN1 SGDID:S000000362, Chr II from 556543-558192, Verified ORF, "Protein required for daughter cell separation, multiple mitotic checkpoints, and chromosome stability; contains 12 degenerate leucine-rich repeat motifs; expression is induced by the Mitotic Exit Network (MEN)"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-05_itms_6.05213.05213.2	3.7088	0.1759	98.9%	1827.8562	1829.9293	2	4.8	53.3%	2	G.FAGCDVDDAGIWEFAR.L	2

Reverse_YPR127W

345

38601

6.0

YPR127W SGDID:S000006331, Chr XVI from 790079-791116, Uncharacterized ORF, "Hypothetical protein"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-03_itms_10.07042.07042.2	2.0913	0.1189	91.2%	1088.6372	1086.19	42	4.162	66.7%	1	L.QALTISNNQP.R	2

Reverse_YML041C

280

32021

9.5

VPS71 SGDID:S000004505, Chr XIII from 195755-194913, reverse complement, Verified ORF, "Protein of unknown function, component of the Swr1p complex that incorporates Htz1p into chromatin; required for vacuolar protein sorting"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-03_itms_18.11781.11781.2	1.372	0.1672	95.1%	898.6111	896.90497	132	4.338	57.1%	1	N.GCNVCSSI.S	2

YNL126W

2.8%

846

98227

7.5

SPC98 SGDID:S000005070, Chr XIV from 387229-389769, Verified ORF, "Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-04_itms_7.06162.06162.2	2.4347	0.2205	98.2%	1233.6627	1234.3977	2	4.61	72.2%	1	K.SQNQFDLIRL.N	2
*	02142006-Holinger-05_itms_8.06083.06083.2	3.3729	0.2119	99.3%	1631.7946	1632.7673	1	5.036	69.2%	1	K.TLNIDELESVHNTF.L	2

Reverse_YJR062C

2.8%

457

51897

5.2

NTA1 SGDID:S000003823, Chr X from 554763-553390, reverse complement, Verified ORF, "Amidase, removes the amide group from N-terminal asparagine and glutamine residues to generate proteins with N-terminal aspartate and glutamate residues that are targets of ubiquitin-mediated degradation"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-04_itms_11.08396.08396.2	2.5056	0.0616	93.5%	1550.7805	1551.7771	2	4.256	75.0%	1	L.FTKELVTDKIVEE.S	2

YHR143W

2.8%

325

33417

4.5

DSE2 SGDID:S000001186, Chr VIII from 385513-386490, Verified ORF, "Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-06_itms_6.05115.05115.2	2.2388	0.1015	95.6%	876.5237	876.98236	40	4.364	62.5%	2	T.STLVPSSSV.D	2

YMR001C

2.7%

705

81031

9.0

CDC5 SGDID:S000004603, Chr XIII from 271136-269019, reverse complement, Verified ORF, "Polo-like kinase with similarity to Xenopus Plx1 and S. pombe Plo1p; found at bud neck, nucleus and SPBs; has multiple functions in mitosis and cytokinesis through phosphorylation of substrates; may be a Cdc28p substrate"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-06_itms_9.06739.06739.2	3.9725	0.2952	100.0%	2194.046	2194.3594	1	6.028	52.8%	2	M.SEAPNFEDIPEEQSLVNFK.D	2

Reverse_YER068W

2.7%

587

65354

8.1

MOT2 SGDID:S000000870, Chr V from 293048-294811, Verified ORF, "Component of the CCR4-NOT transcription regulatory complex, which represses transcription, at least in part, by inhibiting functional TBP-DNA interactions and also aids in transcription elongation; interacts with C-terminal region of Not1p"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-05_itms_7.05792.05792.2	2.6398	0.1312	90.8%	1721.8098	1721.821	1	4.147	53.3%	1	N.ETTPTTHHGLSDNLTP.L	2

U	YDR385W	3	3	2.6%	842	93289	6.3	EFT2 SGDID:S000002793, Chr IV from 1243220-1245748, Verified ORF, "Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin"
U	YOR133W	3	3	2.6%	842	93289	6.3	EFT1 SGDID:S000005659, Chr XV from 575098-577626, Verified ORF, "Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin"

Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
02142006-Holinger-06_itms_9.06912.06912.2	2.6739	0.1571	95.7%	1210.5927	1211.3623	2	4.383	83.3%	1	R.LWGDSFFNPK.T	2
02142006-Holinger-04_itms_7.05952.05952.2	3.1977	0.2804	100.0%	1282.7037	1283.4673	1	5.766	77.3%	1	K.NANDLPKLVEGL.K	2
02142006-Holinger-04_itms_7.05808.05808.2	2.4235	0.243	99.1%	1097.6237	1098.2847	9	4.843	72.2%	1	A.NDLPKLVEGL.K	2

Reverse_YEL041W

2.6%

495

55874

5.8

YEL041W SGDID:S000000767, Chr V from 75944-77431, Uncharacterized ORF, "Hypothetical protein"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-06_itms_6.05013.05013.2	2.3481	0.248	99.1%	1351.6135	1352.3948	1	4.863	58.3%	1	Y.LNSTDEGVITESS.L	2

YMR068W

2.6%

426

47140

9.3

AVO2 SGDID:S000004672, Chr XIII from 406303-407583, Verified ORF, "Component of a complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-03_itms_17.11250.11250.2	1.8279	0.0579	90.5%	1135.7815	1133.1167	10	4.137	55.0%	1	D.SFSSSSNSSSR.L	2

YGR279C

2.6%

386

40173

4.8

SCW4 SGDID:S000003511, Chr VII from 1049964-1048804, reverse complement, Verified ORF, "Cell wall protein with similarity to glucanases; scw4 scw10 double mutants exhibit defects in mating"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-04_itms_6.05196.05196.2	3.3013	0.1116	99.3%	1159.6505	1160.3585	6	5.031	77.8%	2	L.AQLTDFPVIR.L	2

YCR089W

1609

166036

4.9

FIG2 SGDID:S000000685, Chr III from 267430-272259, Verified ORF, "Cell wall adhesin, expressed specifically during mating; may be involved in maintenance of cell wall integrity during mating"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-05_itms_13.09540.09540.3	2.4394	0.2599	100.0%	4363.492	4365.0146	9	4.527	17.1%	1	L.LFPALSTEMINTTVVSRKTLIISTEVCSHSKCVPTVITE.V	3

YIL014W

630

72410

5.6

MNT3 SGDID:S000001276, Chr IX from 326101-327993, Verified ORF, "Alpha-1,3-mannosyltransferase, adds the fourth and fifth alpha-1,3-linked mannose residues to O-linked glycans during protein O-glycosylation"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-03_itms_9.06777.06777.2	2.5602	0.1724	90.3%	1692.8535	1691.879	29	4.122	42.9%	1	E.SIAKISETAKETEQR.V	2

YBR248C

552

61068

5.5

HIS7 SGDID:S000000452, Chr II from 716460-714802, reverse complement, Verified ORF, "Imidazole glycerol phosphate synthase (glutamine amidotransferase:cyclase), catalyzes the fifth and sixth steps of histidine biosynthesis and also produces 5-aminoimidazole-4-carboxamide ribotide (AICAR), a purine precursor"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-06_itms_20.13738.13738.2	1.8677	0.1606	98.0%	1394.709	1395.5522	6	4.595	45.8%	1	G.SVESPKSTGLNYI.D	2

YBL045C

457

50228

7.3

COR1 SGDID:S000000141, Chr II from 135519-134146, reverse complement, Verified ORF, "Core subunit of the ubiquinol-cytochrome c reductase complex (bc1 complex), which is a component of the mitochondrial inner membrane electron transport chain"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-04_itms_5.04665.04665.2	1.9539	0.0614	91.3%	1112.6202	1113.1693	1	4.164	65.0%	1	L.AVEGEPVNSPN.Y	2

YNL009W

420

47856

9.2

IDP3 SGDID:S000004954, Chr XIV from 614822-616084, Verified ORF, "Peroxisomal NADP-dependent isocitrate dehydrogenase, catalyzes oxidation of isocitrate to alpha-ketoglutarate with the formation of NADP(H+), required for growth on unsaturated fatty acids"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-03_itms_19.12340.12340.2	1.2381	0.06	90.6%	963.7325	963.1982	1	4.131	61.1%	1	F.PKSGGIAMAM.F	2

YKL072W

766

88836

8.6

STB6 SGDID:S000001555, Chr XI from 299226-301526, Verified ORF, "Protein that binds Sin3p in a two-hybrid assay"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-04_itms_6.05585.05585.2	3.3512	0.1842	94.2%	2086.9712	2085.556	114	4.307	41.2%	1	D.IFKFKKVVKSTVQDMTGK.G	2

YJL019W

682

79175

5.3

MPS3 SGDID:S000003556, Chr X from 402813-404861, Verified ORF, "Essential integral membrane protein required for spindle pole body duplication and for nuclear fusion, localizes to the spindle pole body half bridge, interacts with DnaJ-like chaperone Jem1p and with centrin homolog Cdc31p"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-06_itms_11.08496.08496.2	3.1075	0.3291	100.0%	1801.9435	1803.0221	1	6.103	63.3%	1	R.LLPSNLVNFENDINSL.T	2

YER143W

428

47354

5.1

DDI1 SGDID:S000000945, Chr V from 456314-457600, Verified ORF, "DNA damage-inducible v-SNARE binding protein, contains a ubiquitin-associated (UBA) domain, may act as a negative regulator of constitutive exocytosis, may play a role in S-phase checkpoint control"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-05_itms_6.05062.05062.2	2.1091	0.172	92.4%	1073.5289	1074.2163	192	4.205	66.7%	1	L.DTDIDVLIGL.D	2

YGL075C

387

44585

8.3

MPS2 SGDID:S000003043, Chr VII from 368091-366928, reverse complement, Verified ORF, "Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required for insertion of the newly duplicated SPB into the nuclear envelope; potentially phosphorylated by Cdc28p"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-05_itms_7.05775.05775.2	2.6962	0.1938	99.3%	1013.0403	1014.2505	38	4.917	75.0%	1	K.LTESPIKLL.S	2

Reverse_YOR239W

2.2%

628

71486

4.7

ABP140 SGDID:S000005765, Chr XV from 784857-785687,785689-786744, Verified ORF, "Nonessential protein that binds actin filaments and localizes to actin patches and cables, has similarity to S-adenosylmethionine (AdoMet)-dependent methyltransferases"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-01_itms_17.10882.10882.2	1.6681	0.0283	95.5%	1560.9424	1562.8671	415	4.377	42.3%	1	A.SFVFIMVAIDVSHP.E	2

YOR025W

2.2%

447

50524

9.1

HST3 SGDID:S000005551, Chr XV from 378218-379561, Verified ORF, "Member of the Sir2 family of NAD(+)-dependent protein deacetylases; involved along with Hst4p in telomeric silencing, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-01_itms_17.10983.10983.2	2.0297	0.0708	94.1%	1048.6483	1050.1407	1	4.296	66.7%	2	S.PPASRSGSMC.S	2

U	Reverse_YHR179W	1	1	2.2%	400	45011	6.6	OYE2 SGDID:S000001222, Chr VIII from 462502-463704, Verified ORF, "Widely conserved NADPH oxidoreductase containing flavin mononucleotide (FMN), homologous to Oye3p with slight differences in ligand binding and catalytic properties; may be involved in sterol metabolism"
U	Reverse_YPL171C	1	1	2.2%	400	44920	5.6	OYE3 SGDID:S000006092, Chr XVI from 227370-226168, reverse complement, Verified ORF, "Widely conserved NADPH oxidoreductase containing flavin mononucleotide (FMN), homologous to Oye2p with slight differences in ligand binding and catalytic properties; may be involved in sterol metabolism"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
	02142006-Holinger-03_itms_6.05132.05132.2	2.0587	0.1134	92.5%	1049.5402	1050.1998	113	4.214	62.5%	1	V.ADVVELTFR.A	2

Reverse_YMR094W

2.1%

478

56336

9.1

CTF13 SGDID:S000004700, Chr XIII from 455824-457260, Verified ORF, "Subunit of the CBF3 complex, which binds to the CDE III element of centromeres, bending the DNA upon binding, and may be involved in sister chromatid cohesion during mitosis"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-04_itms_6.05217.05217.2	2.5875	0.2156	99.4%	1205.6545	1206.4056	439	5.373	55.6%	1	G.TRSIQNMRSL.R	2

Reverse_YLR337C

817

82594

9.9

VRP1 SGDID:S000004329, Chr XII from 805106-802653, reverse complement, Verified ORF, "Proline-rich, actin-associated protein involved in cytoskeletal organization and cytokinesis; related to mammalian Wiskott-Aldrich syndrome protein (WASP)-interacting protein (WIP)"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-06_itms_19.12945.12945.2	1.9147	0.221	97.1%	1561.8734	1563.8369	33	4.526	40.0%	1	V.NPLSPPPPATPPLPPA.H	2

YHR015W

659

75920

9.4

MIP6 SGDID:S000001057, Chr VIII from 134547-136526, Verified ORF, "Putative RNA-binding protein, interacts with Mex67p, which is a component of the nuclear pore involved in nuclear mRNA export"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-03_itms_5.04364.04364.2	2.4046	0.1943	98.2%	1471.7794	1474.6965	1	4.641	54.2%	1	L.DYFSTIGPIKSVF.I	2

YML031W

655

74134

8.3

NDC1 SGDID:S000004493, Chr XIII from 214189-216156, Verified ORF, "Nuclear envelope protein with multiple putative transmembrane domains, required for nuclear pore complex assembly and spindle pole body duplication; required for meiosis II"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-05_itms_8.06081.06081.2	4.0204	0.2853	100.0%	1626.7941	1627.7966	1	5.803	79.2%	1	E.NFFQAPQDYVLER.V	2

Reverse_YIR039C

537

58214

4.3

YPS6 SGDID:S000001478, Chr IX from 432107-430494, reverse complement, Verified ORF, "Putative GPI-anchored aspartic protease"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-03_itms_12.08727.08727.2	1.7074	0.1203	97.5%	1104.7222	1104.3502	2	4.549	60.0%	1	L.IAVSQATIIMG.P	2

Reverse_YCR045C

491

55068

5.4

YCR045C SGDID:S000000641, Chr III from 209605-208130, reverse complement, Uncharacterized ORF, "Hypothetical protein"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-05_itms_11.08424.08424.2	2.5033	0.1115	90.1%	1275.6887	1276.4412	60	4.114	66.7%	1	V.KTCHEVIFEL.G	2

YDL063C

1.9%

620

70164

5.2

YDL063C SGDID:S000002221, Chr IV from 340134-338272, reverse complement, Uncharacterized ORF, "Hypothetical protein"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-05_itms_12.08511.08511.2	2.1445	0.2279	97.3%	1297.6708	1298.6061	417	4.526	50.0%	1	E.IAIDLITAIIEI.V	2

YLL024C

1.9%

639

69470

5.1

SSA2 SGDID:S000003947, Chr XII from 97484-95565, reverse complement, Verified ORF, "ATP binding protein involved in protein folding and vacuolar import of proteins; member of heat shock protein 70 (HSP70) family; associated with the chaperonin-containing T-complex; present in the cytoplasm, vacuolar membrane and cell wall"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-06_itms_10.07282.07282.2	3.7725	0.3305	100.0%	1362.7573	1363.5939	1	6.605	81.8%	1	S.LLSIEDGIFEVK.A	2
*	02142006-Holinger-03_itms_6.04862.04862.2	1.9627	0.0687	98.9%	1136.5867	1137.275	97	4.808	61.1%	1	L.SIEDGIFEVK.A	2

Reverse_YDR458C

1.8%

663

76377

7.8

YDR458C SGDID:S000002866, Chr IV from 1382036-1380045, reverse complement, Uncharacterized ORF, "Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nuclear periphery"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-06_itms_6.05194.05194.2	2.3587	0.1283	93.4%	1357.6837	1358.7942	49	4.255	59.1%	1	Q.IKKILFLVGLTI.F	2

Reverse_YIL155C

1.8%

649

72389

7.9

GUT2 SGDID:S000001417, Chr IX from 53708-51759, reverse complement, Verified ORF, "Mitochondrial glycerol-3-phosphate dehydrogenase; expression is repressed by both glucose and cAMP and derepressed by non-fermentable carbon sources in a Snf1p, Rsf1p, Hap2/3/4/5 complex dependent manner"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-06_itms_16.11472.11472.2	1.6304	0.1661	95.8%	1146.7457	1147.2737	78	4.424	50.0%	1	R.VVGQTASGKKGD.A	2

YER020W

1.8%

449

50448

6.4

GPA2 SGDID:S000000822, Chr V from 195167-196516, Verified ORF, "Nucleotide binding alpha subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p, has signaling role in response to nutrients; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-02_itms_4.03490.03490.2	2.5641	0.0983	90.3%	968.4945	969.03613	7	4.122	78.6%	1	S.LSEYDQTL.M	2

Reverse_YNL298W

842

93909

9.1

CLA4 SGDID:S000005242, Chr XIV from 68915-71443, Verified ORF, "Cdc42p activated signal transducing kinase of the PAK (p21-activated kinase) family, involved in septin ring assembly and cytokinesis; directly phosphorylates septins Cdc3p and Cdc10p; other yeast PAK family members are Ste20p and Skm1p"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-04_itms_7.06260.06260.2	2.8173	0.0062	91.0%	1517.7671	1518.812	2	4.155	57.7%	1	Q.SPDANVTVKKLKSM.I	2

YHL024W

713

80109

5.8

RIM4 SGDID:S000001016, Chr VIII from 56647-58788, Verified ORF, "Putative RNA-binding protein required for the expression of early and middle sporulation genes"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-01_itms_16.10232.10232.2	1.708	0.1803	94.2%	1167.7074	1168.3502	179	4.306	45.5%	1	P.PPSGLDGSMIPP.P	2

YML020W

664

76138

8.1

YML020W SGDID:S000004482, Chr XIII from 231149-233143, Uncharacterized ORF, "Hypothetical protein"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-04_itms_6.05242.05242.2	2.1799	0.2163	92.3%	1189.5491	1192.4008	1	4.208	70.0%	1	I.ISNNVKITFVG.S	2

YDR049W

632

72733

8.9

YDR049W SGDID:S000002456, Chr IV from 553251-555149, Uncharacterized ORF, "Hypothetical protein"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-02_itms_17.10998.10998.2	2.3429	0.1506	90.7%	1276.6919	1277.4601	105	4.141	55.0%	1	E.TLIEQAVNFLE.H	2

YPR036W

478

54416

6.4

VMA13 SGDID:S000006240, Chr XVI from 643833-645269, Verified ORF, "Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-01_itms_26.15955.15955.2	1.5665	0.0264	91.6%	887.7318	885.9927	19	4.174	78.6%	1	L.IHLLSTSD.N	2

Reverse_YPL161C

1.6%

633

70993

5.0

BEM4 SGDID:S000006082, Chr XVI from 246219-244318, reverse complement, Verified ORF, "Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-04_itms_8.06563.06563.2	2.8893	0.1066	92.3%	1088.6064	1090.3325	8	4.209	77.8%	2	S.NCLIMLAAAL.A	2

Reverse_YDL003W

1.6%

566

63290

4.6

MCD1 SGDID:S000002161, Chr IV from 444680-446380, Verified ORF, "Essential protein required for sister chromatid cohesion in mitosis and meiosis; subunit of the cohesin complex; expression is cell cycle regulated and peaks in S phase "

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-04_itms_14.10399.10399.2	1.7376	0.2033	91.8%	926.68427	926.0605	267	4.187	62.5%	1	S.AVEGPTPRV.K	2

YBL105C

1.5%

1151

131519

7.1

PKC1 SGDID:S000000201, Chr II from 17696-14241, reverse complement, Verified ORF, "Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-05_itms_7.05963.05963.2	3.2384	0.069	92.9%	2097.0261	2099.3916	1	4.231	53.1%	1	V.EKQYQEANTKLTKLYQI.D	2

YER125W

1.5%

809

91816

6.7

RSP5 SGDID:S000000927, Chr V from 410185-412614, Verified ORF, "Ubiquitin-protein ligase involved in ubiquitin-mediated protein degradation; plays a role in heat shock element (HSE)-mediated gene expression and multivesicular body sorting; contains a hect (homologous to E6-AP carboxyl terminus) domain"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-06_itms_10.07835.07835.2	2.2165	0.194	99.1%	1362.7133	1363.5559	4	4.826	68.2%	1	R.DVFRSPDPFAVL.T	2

YPR175W

1.5%

689

78340

6.2

DPB2 SGDID:S000006379, Chr XVI from 888970-891039, Verified ORF, "Second largest subunit of DNA polymerase II (DNA polymerase epsilon), required for normal yeast chromosomal replication; expression peaks at the G1/S phase boundary; potential Cdc28p substrate"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-04_itms_12.09001.09001.2	1.7409	0.0338	90.6%	1146.6442	1147.3195	312	4.131	61.1%	1	N.LLGRDAQNFL.L	2

U	Reverse_YBR299W	1	1	1.5%	584	68142	5.7	MAL32 SGDID:S000000503, Chr II from 805345-807099, Verified ORF, "Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL3 complex locus; functional in genomic reference strain S288C"
U	Reverse_YGR292W	1	1	1.5%	584	68094	5.7	MAL12 SGDID:S000003524, Chr VII from 1076605-1078359, Verified ORF, "Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL1 complex locus"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
	02142006-Holinger-01_itms_14.08778.08778.2	1.7035	0.1831	90.2%	901.45557	901.0252	35	4.115	62.5%	1	S.GHAVEGVTM.I	2

Reverse_YKL173W

1008

114041

5.9

SNU114 SGDID:S000001656, Chr XI from 122522-125548, Verified ORF, "GTPase component of U5 snRNP involved in mRNA splicing via spliceosome; binds directly to U5 snRNA; proposed to be involved in conformational changes of the spliceosome; similarity to ribosomal translocation factor EF-2"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-02_itms_6.05184.05184.2	3.0297	0.1617	92.6%	1504.7482	1505.6648	94	4.222	53.8%	1	R.LDTEFGASEIVPVQ.G	2

YIL108W

696

77414

7.5

YIL108W SGDID:S000001370, Chr IX from 160884-162974, Uncharacterized ORF, "Putative metalloprotease"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-05_itms_7.05554.05554.2	3.9276	0.2657	99.3%	1219.6621	1221.3989	1	4.917	88.9%	10	G.SKDIDLFNLR.E	2

Reverse_YNR008W

661

75393

6.7

LRO1 SGDID:S000005291, Chr XIV from 640398-642383, Verified ORF, "Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis; putative homolog of human lecithin cholesterol acyltransferase"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-05_itms_17.11437.11437.2	1.7002	0.2229	96.1%	842.51263	843.01105	310	4.472	56.2%	1	E.IGTSIVGPV.M	2

YEL036C

500

58182

5.3

ANP1 SGDID:S000000762, Chr V from 84552-83050, reverse complement, Verified ORF, "Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-03_itms_19.12170.12170.2	1.2386	0.0923	91.1%	810.56726	812.76495	7	4.155	66.7%	1	I.YEPSSDD.L	2

YMR280C

1433

160485

9.0

CAT8 SGDID:S000004893, Chr XIII from 831328-827027, reverse complement, Verified ORF, "Zinc cluster transcriptional activator necessary for derepression of a variety of genes under non-fermentative growth conditions, active after diauxic shift, binds carbon source responsive elements"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-06_itms_8.06386.06386.2	2.8266	0.1424	92.6%	1762.7714	1763.9047	63	4.22	38.2%	2	A.SADPGTNKKAVTNAGANF.K	2

YKL215C

1286

140427

6.8

YKL215C SGDID:S000001698, Chr XI from 30688-26828, reverse complement, Uncharacterized ORF, "Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-05_itms_11.08337.08337.2	2.2646	0.1743	96.1%	1779.885	1778.842	361	4.471	34.4%	1	K.SSPNEQEGILEGNSGEM.V	2

Reverse_YPL160W

1090

124141

5.8

CDC60 SGDID:S000006081, Chr XVI from 246989-250261, Verified ORF, "Cytosolic leucyl tRNA synthetase, ligates leucine to the appropriate tRNA"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-05_itms_13.09355.09355.2	2.0257	0.0879	96.6%	1501.7227	1502.7942	232	4.512	46.2%	1	R.LYDLAALVGKDVPK.S	2

Reverse_YNL267W

1066

119923

6.5

PIK1 SGDID:S000005211, Chr XIV from 140879-144079, Verified ORF, "Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokineses through the actin cytoskeleton"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-05_itms_8.06314.06314.2	2.4556	0.1987	93.2%	1485.0029	1484.5626	21	4.252	50.0%	1	F.HDNLSAIGGKDDLE.A	2

YLR249W

1044

115945

6.0

YEF3 SGDID:S000004239, Chr XII from 636782-639916, Verified ORF, "Translational elongation factor, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing EF-1 alpha from the ribosomal complex; contains two ABC cassettes; binds and hydrolyses ATP"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-06_itms_9.06834.06834.2	2.6395	0.161	98.9%	1525.8683	1526.8162	1	4.779	69.2%	1	K.LVEDPQVIAPFLGK.L	2

YDR475C

876

98691

9.6

YDR475C SGDID:S000002883, Chr IV from 1410084-1407454, reverse complement, Uncharacterized ORF, "Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-03_itms_7.05527.05527.2	3.3706	0.1515	91.8%	1207.737	1206.3011	8	4.177	75.0%	1	V.KSNDSNSGKRI.K	2

Reverse_YLL054C

769

89163

7.8

YLL054C SGDID:S000003977, Chr XII from 35203-32894, reverse complement, Uncharacterized ORF, "Hypothetical protein"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-06_itms_6.05008.05008.2	1.573	0.1048	91.5%	1095.543	1095.1974	6	4.172	50.0%	1	T.LNNSKVGFSE.K	2

Reverse_YJL103C

618

70382

7.9

YJL103C SGDID:S000003639, Chr X from 230798-228942, reverse complement, Uncharacterized ORF, "Hypothetical protein"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-01_itms_27.16482.16482.2	1.7509	0.1789	91.3%	867.57007	869.9134	33	4.165	71.4%	1	T.IKSSTSSC.A	2

Reverse_YKL204W

632

69762

9.5

EAP1 SGDID:S000001687, Chr XI from 53705-55603, Verified ORF, "eIF4E-associated protein, binds eIF4E and inhibits cap-dependent translation, also functions independently of eIF4E to maintain genetic stability; plays a role in cell growth, implicated in the TOR signaling cascade"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-01_itms_7.04895.04895.1	1.0454	0.2275	97.0%	815.21497	814.87354	1	4.972	50.0%	1	S.KHTAAATD.S	1

YKR095W

1875

218454

5.2

MLP1 SGDID:S000001803, Chr XI from 619447-625074, Verified ORF, "Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved with Tel1p in telomere length control; involved with Pml1p and Pml39p in nuclear retention of unspliced mRNAs"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-06_itms_10.07492.07492.2	3.4127	0.3172	99.7%	2369.2441	2370.6218	1	5.377	40.9%	1	Y.TAADKEVNNSTNGPGLNNILITL.R	2

Reverse_YDR159W

1301

149568

8.9

SAC3 SGDID:S000002566, Chr IV from 771872-775777, Verified ORF, "Nuclear pore-associated protein, forms a complex with Thp1p that is involved in transcription and in mRNA export from the nucleus"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-04_itms_10.07794.07794.2	2.6798	0.2464	97.9%	1689.8328	1690.9086	16	4.578	42.9%	1	S.FILNKDSSSLMFSSN.V	2

YDR017C

1050

119550

6.8

KCS1 SGDID:S000002424, Chr IV from 482263-479111, reverse complement, Verified ORF, "Inositol hexaphosphate kinase, phosphorylates inositol hexakisphosphate (InsP6) to diphosphoinositol polyphosphates, required for proper vacuole morphology and involved in salt stress response, contains two leucine heptad repeats"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-05_itms_10.07737.07737.2	3.4237	0.1042	90.4%	1563.8369	1565.776	6	4.117	62.5%	1	K.VNLIDFARCVTKE.D	2

YNL278W

1060

118280

8.9

CAF120 SGDID:S000005222, Chr XIV from 113271-116453, Verified ORF, "Part of the evolutionarily-conserved CCR4-NOT transcriptional regulatory complex involved in controlling mRNA initiation, elongation, and degradation"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-06_itms_10.07358.07358.2	2.4913	0.1755	91.1%	1348.7443	1350.4711	27	4.155	54.2%	1	L.GSADLFERADGVL.S	2

YFR029W

678

76286

7.5

PTR3 SGDID:S000001925, Chr VI from 210925-212961, Verified ORF, "Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-06_itms_7.05720.05720.2	1.4515	0.0999	98.2%	955.5288	953.0422	387	4.67	57.1%	1	E.YGPNFNQL.T	2

YNL299W

642

74179

7.0

TRF5 SGDID:S000005243, Chr XIV from 66517-68445, Verified ORF, "Poly (A) polymerase involved in nuclear RNA quality control based on: homology with Trf4p, genetic interactions with TRF4 mutants, physical interaction with Mtr4p (TRAMP subunit), and by direct assay; disputed role as a sigma DNA polymerase"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-04_itms_7.06261.06261.2	2.2261	0.1298	92.0%	926.54425	926.19055	270	4.188	78.6%	1	V.IVKTRVPI.I	2

YPL242C

1495

172830

8.9

IQG1 SGDID:S000006163, Chr XVI from 95109-90622, reverse complement, Verified ORF, "Essential protein required for determination of budding pattern, promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-03_itms_5.04381.04381.2	2.7795	0.1226	96.1%	1822.787	1824.0488	46	4.467	43.3%	1	K.DFSPVHKSKFGNFKNA.V	2

Reverse_YMR129W

1337

151652

6.7

POM152 SGDID:S000004736, Chr XIII from 527803-531816, Verified ORF, "Nuclear pore membrane glycoprotein; may be involved in duplication of nuclear pores and nuclear pore complexes during S-phase;"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-04_itms_7.06185.06185.2	3.1559	0.1072	90.5%	1781.8633	1783.9817	435	4.14	46.4%	1	E.KSFLNETVKTIHHND.Q	2

YKL197C

1043

117276

7.0

PEX1 SGDID:S000001680, Chr XI from 73870-70739, reverse complement, Verified ORF, "AAA-family ATPase peroxin required for peroxisome biogenesis, contains two 230 amino acid ATP-binding AAA cassettes, upregulated in anaerobiosis; Pex1p and Pex6p interact via their N-terminal AAA-cassettes"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-05_itms_5.04634.04634.2	2.6744	0.2349	99.2%	1195.5776	1198.2804	1	5.064	70.0%	1	Q.EFGIAVHSHNS.D	2

Reverse_YLR014C

904

102724

7.4

PPR1 SGDID:S000004004, Chr XII from 174981-172267, reverse complement, Verified ORF, "Zinc finger transcription factor containing a Zn(2)-Cys(6) binuclear cluster domain, positively regulates transcription of genes involved in uracil biosynthesis; activity may be modulated by interaction with Tup1p"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-05_itms_6.05313.05313.2	1.8894	0.1379	92.8%	1196.6329	1197.463	9	4.229	66.7%	1	P.IHFKTPLEVI.P	2

YLR219W

728

80530

9.1

MSC3 SGDID:S000004209, Chr XII from 574153-576339, Verified ORF, "Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; msc3 mutants are defective in directing meiotic recombination events to homologous chromatids; potential Cdc28p substrate"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-05_itms_12.08812.08812.2	1.4258	0.2636	93.9%	828.51276	830.7862	75	4.278	57.1%	1	Y.HTDAASDN.A	2

Reverse_YKL213C

715

79506

5.0

DOA1 SGDID:S000001696, Chr XI from 34108-31961, reverse complement, Verified ORF, "WD repeat protein required for ubiquitin-mediated protein degradation, forms complex with Cdc48p, plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-01_itms_28.17167.17167.1	1.4359	0.2598	91.2%	733.35406	733.7563	8	4.925	57.1%	1	K.KDNGTAGA.G	1

Reverse_YMR280C

1433

160485

9.0

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-05_itms_7.05632.05632.2	3.336	0.2479	96.9%	1679.792	1680.8855	12	4.522	50.0%	1	K.SDMNDKMLDLNSPLS.F	2

YPL217C

1183

135571

6.8

BMS1 SGDID:S000006138, Chr XVI from 143170-139619, reverse complement, Verified ORF, "Essential conserved nucleolar GTP-binding protein required for synthesis of 40S ribosomal subunits and for processing of the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, has similarity to Tsr1p"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-06_itms_11.08077.08077.2	1.9043	0.0949	93.4%	1303.5824	1305.2108	395	4.255	50.0%	1	L.EDGNPSEQAEDN.S	2

Reverse_YGL049C

914

103899

7.6

TIF4632 SGDID:S000003017, Chr VII from 409607-406863, reverse complement, Verified ORF, "Translation initiation factor eIF4G, subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); associates with the poly(A)-binding protein Pab1p, also interacts with eIF4A (Tif1p); homologous to Tif4631p"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-03_itms_5.04620.04620.2	1.7985	0.0601	96.4%	1028.5641	1029.011	250	4.493	68.8%	1	R.NSSYRNSNS.Q	2

Reverse_YIL169C

995

99736

4.6

YIL169C SGDID:S000001431, Chr IX from 26106-23119, reverse complement, Uncharacterized ORF, "Hypothetical protein"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-02_itms_17.11201.11201.2	1.476	0.111	97.3%	1077.7306	1075.1174	393	4.537	55.6%	1	T.YSQSSVTTTT.T	2

YKL073W

881

99572

5.3

LHS1 SGDID:S000001556, Chr XI from 296074-298719, Verified ORF, "Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; member of the Hsp70 family; localizes to the lumen of the ER; regulated by the unfolded protein response pathway"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-05_itms_6.05041.05041.2	2.4506	0.1662	95.8%	955.5271	957.0306	39	4.417	75.0%	1	K.QFGGYGTNL.P	2

YBR098W

691

78764

7.5

MMS4 SGDID:S000000302, Chr II from 441509-443584, Verified ORF, "Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in recombination and DNA repair"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-04_itms_6.05316.05316.2	2.4481	0.0784	90.6%	807.4647	807.9219	38	4.134	83.3%	2	M.SQIVDFV.E	2

U	YHL035C	1	1	0.9%	1592	180925	8.5	YHL035C SGDID:S000001027, Chr VIII from 32754-27976, reverse complement, Uncharacterized ORF, "Protein of unknown function, member of the ATP-binding cassette (ABC) family, potential Cdc28p substrate"
U	YLL048C	1	1	0.8%	1661	189161	8.1	YBT1 SGDID:S000003971, Chr XII from 46264-41279, reverse complement, Verified ORF, "Transporter of the ATP-binding cassette (ABC) family involved in bile acid transport; similar to mammalian bile transporters"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
	02142006-Holinger-01_itms_17.10894.10894.2	1.8439	0.2374	93.6%	1387.8408	1390.5333	15	4.272	42.3%	1	L.TEIGEKGITLSGGQ.K	2

Reverse_YDR301W

0.9%

1357

153404

5.9

CFT1 SGDID:S000002709, Chr IV from 1063346-1067419, Verified ORF, "RNA-binding subunit of the mRNA cleavage and polyadenylation factor; involved in poly(A) site recognition and required for both pre-mRNA cleavage and polyadenylation, 51% sequence similarity with mammalian AAUAA-binding subunit of CPSF"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-05_itms_17.11736.11736.2	2.1575	0.2341	98.7%	1300.1857	1297.4045	86	4.761	50.0%	1	V.TSVTGSVEEQFI.E	2

YPR049C

0.9%

1178

135025

5.7

ATG11 SGDID:S000006253, Chr XVI from 664670-661134, reverse complement, Verified ORF, "Peripheral membrane protein required for delivery of aminopeptidase I (Lap4p) to the vacuole in the cytoplasm-to-vacuole targeting pathway; also required for peroxisomal degradation (pexophagy)"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-03_itms_6.05150.05150.2	2.3151	0.1476	90.5%	1256.595	1257.4473	2	4.139	65.0%	1	Q.MLDAHDNSLIK.S	2

Reverse_YMR287C

0.9%

969

110822

9.0

MSU1 SGDID:S000004900, Chr XIII from 845344-842435, reverse complement, Verified ORF, "RNase, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-04_itms_18.12199.12199.2	1.3195	0.0711	95.4%	978.9877	980.10876	30	4.359	56.2%	1	K.LTGRAFSLD.P	2

Reverse_YPR164W

1407

161237

5.9

MMS1 SGDID:S000006368, Chr XVI from 870699-874922, Verified ORF, "Protein likely involved in protection against replication-dependent DNA damage; mutants are sensitive to methyl methanesulfonate (MMS); implicated in regulation of Ty1 transposition"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-04_itms_8.06319.06319.2	2.0793	0.1255	92.0%	1207.6156	1206.2144	121	4.194	50.0%	1	V.QSNRTDNINSG.T	2

Reverse_YDR310C

1062

118201

6.2

SUM1 SGDID:S000002718, Chr IV from 1084310-1081122, reverse complement, Verified ORF, "Transcriptional repressor required for repression of middle sporulation-specific genes during mitosis; regulated by the pachytene checkpoint; a dominant mutation acts as a suppressor of silencing defects of SIR2 mutations"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-05_itms_15.10721.10721.2	1.9432	0.3392	98.2%	960.5913	961.1241	130	4.673	50.0%	7	I.SASRPKPSM.S	2

YKL210W

1024

114266

5.1

UBA1 SGDID:S000001693, Chr XI from 39164-42238, Verified ORF, "Ubiquitin activating enzyme, involved in ubiquitin-mediated protein degradation and essential for viability"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-02_itms_7.05688.05688.1	2.1114	0.2225	100.0%	850.4812	850.90356	16	5.005	64.3%	1	H.GLEDGNFV.R	1

YCR057C

923

103983

5.1

PWP2 SGDID:S000000653, Chr III from 223223-220452, reverse complement, Verified ORF, "Conserved 90S pre-ribosomal component essential for proper endonucleolytic cleavage of the 35 S rRNA precursor at A0, A1, and A2 sites; contains eight WD-repeats; PWP2 deletion leads to defects in cell cycle and bud morphogenesis"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-04_itms_14.10105.10105.1	1.7572	0.123	91.4%	766.5347	767.85956	250	4.916	50.0%	1	E.WLAFGSS.K	1

Reverse_YBL088C

2787

321567

6.9

TEL1 SGDID:S000000184, Chr II from 59379-51016, reverse complement, Verified ORF, "Protein kinase, primarily involved in telomere length regulation; contributes to cell cycle checkpoint control in response to DNA damage; functionally redundant with Mec1p; homolog of human ataxia telangiectasia (ATM) gene"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-06_itms_10.07350.07350.2	3.4213	0.1771	94.9%	2150.0361	2151.5571	2	4.336	41.7%	1	I.ALQVYNALQIISGLRLYKS.C	2

U	YGR109W-B	1	1	0.7%	1547	178419	8.8	YGR109W-B SGDID:S000007347, Chr VII from 707614-708463,708465-712258, transposable_element_gene, "TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition"
U	YIL082W-A	1	1	0.7%	1498	172454	9.2	YIL082W-A SGDID:S000003537, Chr IX from 205632-206482,206484-210129, transposable_element_gene, "Hypothetical protein"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
	02142006-Holinger-04_itms_8.06662.06662.2	2.2967	0.1761	91.2%	1243.6528	1244.4302	103	4.16	65.0%	1	A.QFPIPEEASIL.E	2

YIL159W

1375

156851

7.9

BNR1 SGDID:S000001421, Chr IX from 41825-45952, Verified ORF, "Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNI1"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
	02142006-Holinger-05_itms_15.10440.10440.2	1.2866	0.0081	91.2%	1004.6176	1006.2328	36	4.157	50.0%	2	P.PPPPPPPPLP.Q	2

Reverse_YPR026W

1211

136920

5.4

ATH1 SGDID:S000006230, Chr XVI from 615376-619011, Verified ORF, "Acid trehalase required for utilization of extracellular trehalose"

	Filename	XCorr	DeltCN	Conf%	ObsM+H+	CalcM+H+	SpR	ZScore	Ion%	#	Sequence
*	02142006-Holinger-03_itms_19.12394.12394.2	1.387	0.1706	93.9%	966.5175	966.9395	2	4.287	62.5%	1	L.YSQSSSGHN.L	2

YNL163C