DTASelect v2.0.31 /data/3/taoxu/projects/catherine/jamie/mito/noplus4 /bluefish/people-b/applications/yates/dbase/SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta ProLuCID 1.2.0 in SQT format. --trypstat --modstat -p 1 -e cont -m 0 --fp 0.01 true Use criteria 0.0 Minimum peptide confidence 0.01 Peptide false positive rate 0.0 Minimum protein confidence 1.0 Protein false positive rate 1 Minimum charge state 16 Maximum charge state 0.0 Minimum ion proportion 1000 Maximum Sp rank -1.0 Minimum Sp score Require Modified peptide inclusion Any Tryptic status requirement false Multiple, ambiguous IDs allowed Ignore Peptide validation handling XCorr Purge duplicate peptides by protein false Include only loci with unique peptide true Remove subset proteins Ignore Locus validation handling cont Exclude protein names matching 0 Minimum modified peptides per locus 1000 Minimum redundancy for low coverage loci 1 Minimum peptides per locus Locus Sequence Count Spectrum Count Sequence Coverage Length MolWt pI Validation Status Descriptive Name Unique FileName XCorr DeltCN Conf% M+H+ CalcM+H+ TotalIntensity SpR Prob Score IonProportion Redundancy Sequence YKL042W 16 27 42.1% 363 42271 7.9 U SPC42 SGDID:S000001525, Chr XI from 358119-359210, Verified ORF, "Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane" * Mito_110308.15312.15312.2 4.6664 0.398 100.0 2608.2927 2607.0896 51953.1 1 7.3079257 87.5 3 R.LYDDYYNIPYQYSNPT#PMNR.D * Mito_110308.1804.1804.2 2.4725 0.2444 100.0 1062.5127 1062.4503 4109.1 1 5.600456 92.9 1 K.LSDANST#FK.E * Mito_110308.11741.11741.2 3.277 0.3698 100.0 2597.9128 2597.1477 461871.88 1 6.008609 96.0 2 K.VKDPMVDDDPVS#ENYDQINVPK.H * Mito_110308.11688.11688.3 4.047 0.215 100.0 2598.8655 2597.1477 301643.0 2 4.5244274 58.6 1 K.VKDPMVDDDPVSENY#DQINVPK.H * Mito_110308.19243.19243.2 2.6778 0.382 100.0 1377.8127 1376.6643 394175.0 4 7.030125 70.6 1 R.TLS#VLTNYVMR.S * Mito_110308.18819.18819.3 5.3761 0.3941 100.0 2846.5454 2844.3345 83479.7 1 7.4545937 39.7 1 R.SEDGNNDRMS#PLPSPLNTILPINNR.L * Mito_110308.20328.20328.3 4.0862 0.4155 100.0 2926.2854 2924.3008 115921.9 1 6.8056173 40.3 4 R.SEDGNNDRMS#PLPS#PLNTILPINNR.L * Mito_110308.22842.22842.2 4.7126 0.4821 100.0 2038.9327 2036.9639 270020.8 1 7.5221553 74.1 3 R.MS#PLPS#PLNTILPINNR.L * Mito_110308.20108.20108.2 6.4831 0.4945 100.0 2617.1528 2614.1887 1261213.0 1 9.482809 82.8 1 R.KLS#LNNQLQELQSMMDGDDNIK.L * Mito_110308.19588.19588.3 5.4841 0.5489 100.0 3273.3555 3270.538 129014.2 1 9.117404 50.7 1 R.KLS#LNNQLQELQSMMDGDDNIKLDNVSK.H * Mito_110308.10713.10713.2 5.6044 0.4811 100.0 2336.5728 2332.862 124666.4 2 8.266407 60.7 3 R.DYS#PSSDACLECSNDLYEK.N * Mito_110308.1961.1961.2 4.9566 0.3756 100.0 2417.1926 2412.8284 27179.0 8 6.432883 42.9 2 R.DYS#PSS#DACLECSNDLYEK.N * Mito_110308.8574.8574.3 3.4146 0.2968 100.0 2228.5754 2226.9849 162560.0 2 5.038301 50.0 1 R.VKPENNMSETFAT#PTPNNR.- * Mito_110308.8658.8658.2 4.3957 0.3267 100.0 2229.5728 2226.9849 2201252.0 1 6.6049204 60.7 1 R.VKPENNMSETFAT#PTPNNR.- * Mito_110308.8834.8834.3 4.3557 0.2177 100.0 2230.2253 2226.9849 242696.2 8 5.7357535 46.0 1 R.VKPENNMSETFATPT#PNNR.- * Mito_110308.8917.8917.2 3.8087 0.2383 100.0 2230.6128 2226.9849 158164.5 31 4.8761806 42.3 1 R.VKPENNMSETFATPT#PNNR.- YJR053W 8 14 27.4% 574 66087 5.9 U BFA1 SGDID:S000003814, Chr X from 533941-535665, Verified ORF, "Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pathways that control exit from mitosis" * Mito_110308.24277.24277.3 5.2353 0.5574 100.0 4170.5356 4168.73 144444.1 1 9.610499 46.6 1 K.FSS#DDEGDFLTGFEELEGEAIDETISSNDKESADHPR.F * Mito_110308.25759.25759.3 4.0171 0.5217 100.0 4250.845 4248.6963 109434.9 1 8.072609 42.9 1 K.FS#S#DDEGDFLTGFEELEGEAIDETISSNDKESADHPR.F * Mito_110308.11234.11234.2 3.7698 0.3179 100.0 2484.0928 2482.249 70694.6 1 5.0853457 87.5 1 K.ISPAQYDIVKHDELLT#PGLHR.R * Mito_110308.10938.10938.2 3.0479 0.3829 100.0 1848.1727 1847.7847 263414.2 1 5.950899 77.3 1 R.DWNTQQELDS#FKEK.R * Mito_110308.3954.3954.2 4.4913 0.3374 100.0 1647.3528 1646.7104 11127.1 1 6.2774043 73.9 7 R.HCS#NQNVQLNGPAK.I * Mito_110308.8384.8384.2 3.1018 0.1016 100.0 1936.3727 1934.8269 46984.8 19 3.2230427 48.0 1 K.YS#DLQHARATS#RNQK.V * Mito_110308.10443.10443.3 4.5291 0.4094 100.0 2945.2454 2944.251 54897.7 1 6.714648 50.0 1 R.S#KS#QVNTPFVSNDNDGVYQSTAAQAR.L * Mito_110308.16318.16318.3 2.8903 0.3724 100.0 3533.8755 3533.6755 56161.7 38 5.8095098 25.3 1 K.KLASWFIPRDET#IISVDEETIMDESTVNSK.R YOR373W 29 42 25.7% 851 94104 7.0 U NUD1 SGDID:S000005900, Chr XV from 1036830-1039385, Verified ORF, "Component of the spindle pole body outer plaque, required for exit from mitosis" * Mito_110308.14526.14526.3 3.2029 0.3657 100.0 3152.8455 3152.3367 38858.5 1 5.458743 37.3 1 K.EYESNHDFQDSNFTS#QVVEPAISDSVK.K * Mito_110308.12749.12749.3 3.9382 0.4037 100.0 3153.8955 3152.3367 72090.0 1 6.689379 44.8 1 K.EYESNHDFQDSNFTSQVVEPAISDS#VK.K * Mito_110308.8911.8911.2 4.027 0.4802 100.0 2039.6727 2037.9827 44587.8 1 7.4977784 57.1 1 K.KPPTMTVLNNYS#TVHQK.V * Mito_110308.9078.9078.3 2.5138 0.4424 100.0 2100.0254 2098.9229 224684.7 1 7.5208077 67.4 1 K.VPSGFSGTTATS#HQEAQWK.Q * Mito_110308.15973.15973.2 3.2483 0.2485 100.0 1404.1527 1403.6454 126657.4 1 6.369433 85.0 1 K.DLS#GVLETNTFK.R * Mito_110308.15829.15829.1 2.2181 0.2264 100.0 1404.75 1403.6454 225269.6 1 4.877962 87.5 1 K.DLS#GVLETNTFK.R * Mito_110308.13284.13284.1 2.163 0.1651 100.0 1560.83 1559.7465 222159.4 1 4.0705366 89.5 1 K.DLS#GVLETNTFKR.H * Mito_110308.7801.7801.3 3.3998 0.4457 100.0 2249.4854 2247.97 233441.5 1 6.6258163 63.9 1 K.TTTMQTHENHDT#ISISHSK.D * Mito_110308.20084.20084.3 3.2997 0.3957 100.0 2942.4854 2941.1812 78512.7 2 6.9930196 36.2 1 K.VSSSFS#DDS#DSGPAAEAHDVFDGILQK.Q * Mito_110308.22611.22611.3 3.5348 0.2703 100.0 3022.7654 3021.1475 219215.2 1 5.8349533 48.5 1 K.VSSS#FS#DDS#DSGPAAEAHDVFDGILQK.Q * Mito_110308.9641.9641.2 2.8617 0.306 100.0 1724.3727 1723.7323 10862.4 1 5.80412 52.2 1 K.SNYLVGSYPS#NSNNK.N * Mito_110308.9470.9470.1 2.084 0.1843 100.0 1724.79 1723.7323 13320.5 1 4.5069737 80.0 1 K.SNYLVGSYPS#NSNNK.N * Mito_110308.9441.9441.3 3.8333 0.3529 100.0 3878.9653 3875.6714 17160.3 45 5.052724 26.6 1 K.SNYLVGSYPSNSNNKNNNNNNNNNNNNS#ININNK.D * Mito_110308.6854.6854.2 4.048 0.2791 100.0 2252.9727 2250.923 3552.3 11 6.2146006 46.4 7 K.NNNNNNNNNNNNS#ININNK.D * Mito_110308.1659.1659.3 4.8173 0.267 100.0 2709.7153 2707.1313 27409.6 1 5.4657197 55.9 6 K.NNNNNNNNNNNNS#ININNKDNAR.T * Mito_110308.1178.1178.2 3.7798 0.4452 100.0 2337.6926 2337.0493 6654.6 1 7.634979 60.0 1 K.KIQEEENLANSDDT#PLDTPK.F * Mito_110308.1365.1365.3 4.7667 0.2664 100.0 2339.8154 2337.0493 41649.1 1 5.3805523 64.2 1 K.KIQEEENLANS#DDTPLDTPK.F * Mito_110308.14528.14528.3 6.637 0.483 100.0 3205.2554 3202.4827 174908.0 1 8.266667 51.6 1 K.KIQEEENLANSDDTPLDT#PKFNDLFTK.N * Mito_110308.1181.1181.2 5.1745 0.4866 100.0 2209.3728 2208.9543 6684.2 1 8.681104 82.8 1 K.IQEEENLANSDDTPLDT#PK.F * Mito_110308.1087.1087.3 3.9235 0.3695 100.0 2210.0054 2208.9543 7971.2 1 5.2979965 56.0 1 K.IQEEENLANSDDT#PLDTPK.F * Mito_110308.16064.16064.3 5.0006 0.3746 100.0 3075.2056 3074.3877 114254.9 1 7.0842 47.7 1 K.IQEEENLANSDDTPLDT#PKFNDLFTK.N * Mito_110308.16096.16096.2 3.3359 0.3475 100.0 3078.5728 3074.3877 13816.1 7 5.66294 50.0 2 K.IQEEENLANSDDTPLDT#PKFNDLFTK.N * Mito_110308.16820.16820.3 5.2511 0.5017 100.0 3155.7554 3154.354 123625.4 1 8.851007 54.5 1 K.IQEEENLANSDDT#PLDT#PKFNDLFTK.N * Mito_110308.18840.18840.3 4.6677 0.4181 100.0 3237.0554 3234.3203 15592.3 1 5.8852234 48.3 1 K.IQEEENLANS#DDT#PLDT#PKFNDLFTK.N * Mito_110308.13262.13262.2 3.0345 0.4579 100.0 2303.0327 2301.941 94102.2 1 6.9336853 65.4 1 R.VEDITS#ISEVNTS#FNETEK.Q * Mito_110308.13514.13514.2 4.9675 0.4884 100.0 2303.6328 2301.941 140572.5 1 8.845834 76.0 1 R.VEDITSIS#EVNTS#FNETEK.Q * Mito_110308.1954.1954.2 3.584 0.2784 100.0 2383.4727 2381.9072 15395.0 1 5.5226045 70.8 1 R.VEDITS#IS#EVNTS#FNETEK.Q * Mito_110308.9610.9610.2 3.6547 0.252 100.0 1550.8528 1550.6046 273193.2 1 6.5999374 90.0 1 K.LSGS#PSYDSDWEK.I * Mito_110308.9713.9713.1 2.3253 0.3144 100.0 1553.0 1550.6046 13805.3 4 5.18837 64.7 2 K.LSGS#PSYDSDWEK.I YPL124W 10 30 20.9% 253 29280 9.5 U SPC29 SGDID:S000006045, Chr XVI from 316754-317515, Verified ORF, "Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inner plaque component Spc110p; required for SPB duplication" * Mito_110308.7245.7245.2 3.3672 0.2208 100.0 1217.6727 1216.5358 42210.4 5 5.239404 86.7 13 K.KFQDDT#LNR.V * Mito_110308.1291.1291.2 2.6377 0.2105 100.0 1088.6927 1088.4408 364784.9 1 4.7111754 92.3 6 K.FQDDT#LNR.V * Mito_110308.8292.8292.3 4.693 0.2741 100.0 2034.3254 2032.9335 381571.5 1 5.6592655 63.4 1 K.KLREENFSSNTS#ELGNK.K * Mito_110308.1928.1928.2 3.2586 0.3359 100.0 1906.4127 1904.8385 7246.3 5 6.1676245 44.0 1 K.LREENFSSNT#SELGNK.K * Mito_110308.2581.2581.2 2.6356 0.1962 100.0 1111.2327 1109.5602 15615.7 1 5.4296 92.9 1 R.KVKS#PLDDK.L * Mito_110308.7981.7981.2 2.531 0.165 100.0 983.1727 981.4652 222442.4 6 4.829396 84.6 1 K.VKS#PLDDK.L * Mito_110308.7890.7890.1 1.5463 0.4435 100.0 1250.61 1250.6504 82342.9 13 6.4360876 64.3 1 K.VKS#PLDDKLR.R * Mito_110308.9926.9926.2 5.107 0.4602 100.0 1923.8528 1922.8743 101051.4 1 8.498767 91.7 4 R.SSQIHIENEST#EDILK.I * Mito_110308.9829.9829.3 3.7205 0.3469 100.0 1923.8655 1922.8743 144184.0 2 5.487878 75.0 1 R.SSQIHIENEST#EDILK.I * Mito_110308.1946.1946.2 2.9918 0.2017 100.0 2003.6327 2002.8406 7425.3 1 4.6994457 64.0 1 R.SSQIHIENES#T#EDILK.I YIL002W-A 2 3 18.8% 69 7729 4.7 U YIL002W-A SGDID:S000028835, Chr IX from 350298-350507, Uncharacterized ORF, "Identified by expression profiling and mass spectrometry" * Mito_110308.13291.13291.1 2.4535 0.3114 100.0 1648.73 1648.7764 16610.6 1 5.5729485 94.1 1 K.MDS#LLVQLEDLHR.D * Mito_110308.13202.13202.2 3.3585 0.1 100.0 1651.9927 1648.7764 20918.9 15 4.025297 55.0 2 K.MDS#LLVQLEDLHR.D YNL225C 8 12 17.4% 581 67400 6.0 U CNM67 SGDID:S000005169, Chr XIV from 224470-222725, reverse complement, Verified ORF, "Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration" * Mito_110308.13508.13508.3 3.3849 0.2731 100.0 3296.8755 3295.5266 94934.2 13 4.8208375 31.6 1 R.LDSPVS#ENGEIKDGEPIPQNWLNENHVGK.S * Mito_110308.13007.13007.3 4.3161 0.3074 100.0 3296.9055 3295.5266 28789.2 1 5.463965 35.2 1 R.LDS#PVSENGEIKDGEPIPQNWLNENHVGK.S * Mito_110308.14531.14531.3 4.9891 0.2941 100.0 3379.9753 3375.493 717189.4 41 6.0222993 30.0 3 R.LDS#PVS#ENGEIKDGEPIPQNWLNENHVGK.S * Mito_110308.8833.8833.3 3.4727 0.3506 100.0 2246.9355 2244.8572 221877.6 1 5.8911505 63.6 1 R.DSYEENKSPS#MDQMNYAR.N * Mito_110308.1658.1658.2 2.9857 0.1034 99.1 1815.1927 1813.8115 95535.2 2 4.371732 68.2 1 R.NTSYQES#PGLQERPK.N * Mito_110308.1315.1315.2 2.8581 0.2453 100.0 1277.1527 1276.5933 609872.7 1 4.529336 94.4 3 K.DKS#PIGTDVHK.K * Mito_110308.1856.1856.2 2.898 0.3092 100.0 1405.3127 1404.6882 41718.9 5 4.583086 60.0 1 K.DKS#PIGTDVHKK.D * Mito_110308.1423.1423.3 4.8963 0.3718 100.0 2968.4055 2965.2153 28732.6 13 8.799373 38.6 1 R.ANEQASAQPTDEHTSPDIS#IEDCNGAK.I YLR445W 1 1 14.4% 188 22365 4.9 U YLR445W SGDID:S000004437, Chr XII from 1024185-1024568,1024651-1024833, Uncharacterized ORF, "Hypothetical protein" * Mito_110308.17365.17365.2 3.345 0.2694 100.0 3315.4326 3312.3452 4365.0 20 4.285797 41.7 1 T.T#EVPRQSS#ENDQDNNLERTNS#LKSPDV.T YPR103W 1 1 14.3% 287 31637 6.2 U PRE2 SGDID:S000006307, Chr XVI from 732347-733210, Verified ORF, "20S proteasome beta-type subunit, responsible for the chymotryptic activity of the proteasome" * Mito_110308.34435.34435.3 3.5113 0.3214 100.0 4930.615 4925.027 13403.1 8 5.066011 30.9 1 R.DAYSGGS#VNLYHVTEDGWIY#HGNHDVGELFWKVKEEEGS#FN.N YOR257W 1 1 11.8% 161 18751 4.6 U CDC31 SGDID:S000005783, Chr XV from 811006-811491, Verified ORF, "Component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; interacts with Kar1p" * Mito_110308.10972.10972.2 4.9342 0.4987 100.0 2210.7927 2209.0496 48007.3 1 7.8848934 67.9 1 K.NRSS#LQSGPLNSELLEEQK.Q YPL131W 1 1 11.1% 297 33715 6.8 U RPL5 SGDID:S000006052, Chr XVI from 303120-304013, Verified ORF, "Protein component of the large (60S) ribosomal subunit with similarity to E. coli L18 and rat L5 ribosomal proteins; binds 5S rRNA and is required for 60S subunit assembly" * Mito_110308.18606.18606.3 4.2866 0.3538 100.0 3981.9854 3978.7356 117640.4 2 6.538453 32.9 1 D.EERFS#ELFKGY#LADDIDADSLEDIYTSAHEAIR.A YPL255W 3 3 10.4% 385 45384 6.5 U BBP1 SGDID:S000006176, Chr XVI from 67725-68882, Verified ORF, "Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periphery; forms a complex with a nuclear envelope protein Mps2p and SPB components Spc29p and Kar1p; required for mitotic functions of Cdc5p" * Mito_110308.15210.15210.2 2.4444 0.2999 100.0 1461.4127 1461.633 180756.9 1 5.8239408 80.0 1 K.DALFGTDIS#PSMK.Y * Mito_110308.1352.1352.2 3.4015 0.2048 100.0 1481.4528 1479.4899 36705.5 1 5.1326556 88.9 1 R.YAQDDT#NFS#MK.F * Mito_110308.9562.9562.3 3.0885 0.2449 100.0 1850.8455 1849.8691 147737.6 1 5.5750675 65.9 1 K.VLDQTISHEAELATS#R.E YAL047C 7 7 10.0% 622 72105 5.1 U SPC72 SGDID:S000000045, Chr I from 56858-54990, reverse complement, Verified ORF, "Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them to microtubules; has roles in astral microtubule formation and stabilization" * Mito_110308.21190.21190.3 4.1866 0.2033 100.0 2330.7854 2329.1492 363031.9 64 4.947989 39.6 1 R.FEKT#LDT#QLEIVIEILHK.E * Mito_110308.30540.30540.3 3.427 0.3042 100.0 3596.8455 3594.7532 86580.7 38 5.442783 29.4 1 R.FEKT#LDT#QLEIVIEILHKEYDQFINSIR.L * Mito_110308.21913.21913.2 4.5268 0.1355 100.0 1847.6527 1844.9768 1757260.1 14 4.2040453 54.2 1 K.TLDT#QLEIVIEILHK.E * Mito_110308.31565.31565.3 4.7961 0.3373 100.0 3113.4255 3110.5808 22662.9 27 6.2363014 33.9 1 K.TLDT#QLEIVIEILHKEYDQFINSIR.L * Mito_110308.15001.15001.3 5.2763 0.378 100.0 2693.8455 2693.2551 492746.4 1 6.5325227 60.0 1 K.LNEQSHLLDSLELEENSSS#VIEK.Q * Mito_110308.15058.15058.2 5.5162 0.3855 100.0 2696.7126 2693.2551 49353.7 5 7.082342 51.7 1 K.LNEQSHLLDSLELEENSSS#VIEK.Q * Mito_110308.16467.16467.2 3.2146 0.375 100.0 1327.1127 1326.6453 125827.4 1 6.0787835 88.2 1 R.SKLFNLS#INNP.- YDR356W 10 12 8.8% 944 111782 7.1 U SPC110 SGDID:S000002764, Chr IV from 1186098-1188932, Verified ORF, "Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner" * Mito_110308.16474.16474.2 3.4589 0.3046 100.0 1363.9528 1362.6163 327248.8 1 6.3449035 88.9 1 K.NMEFT#PVGFIK.S * Mito_110308.8397.8397.3 4.3543 0.3541 100.0 3077.5754 3074.4539 241510.9 3 6.4282417 39.7 1 K.RNTTQT#QVVSPTKVPNANNGDENEGPVK.K * Mito_110308.2366.2366.2 2.7008 0.3768 100.0 1383.5327 1383.6515 2077.7 2 7.2587047 65.0 1 R.NT#TQTQVVSPTK.V * Mito_110308.2108.2108.2 3.6489 0.3451 100.0 1383.6327 1383.6515 11972.9 1 5.946111 80.0 1 R.NTT#QTQVVSPTK.V * Mito_110308.1335.1335.2 2.4749 0.2129 100.0 1383.8528 1383.6515 11290.7 1 4.73857 70.0 1 R.NTTQTQVVS#PTK.V * Mito_110308.18323.18323.2 2.7558 0.2201 100.0 1940.5728 1939.836 91391.3 1 4.944556 87.5 1 R.LFSEAS#QFDDSFPEIK.A * Mito_110308.17315.17315.2 4.1165 0.1612 100.0 1942.2927 1939.836 144132.1 1 5.7788243 60.9 2 R.LFSEASQFDDS#FPEIK.A * Mito_110308.19590.19590.2 3.7535 0.4095 100.0 2021.8328 2019.8024 270837.7 1 6.6598935 64.0 1 R.LFSEAS#QFDDS#FPEIK.A * Mito_110308.6168.6168.2 2.1547 0.1995 99.1 931.8927 931.4397 73122.4 4 4.3845677 76.9 2 K.ANIPPS#PR.S * Mito_110308.17847.17847.2 3.8504 0.2715 100.0 2432.0127 2430.166 8834.7 7 4.851539 48.1 1 K.SEQS#NIQHDLNLQILNLENK.L YOR284W 1 1 8.6% 243 28414 9.0 U HUA2 SGDID:S000005810, Chr XV from 848475-849206, Uncharacterized ORF, "Cytoplasmic protein of unknown function; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly" * Mito_110308.13526.13526.2 2.4126 0.3289 100.0 2675.7327 2674.0303 11336.2 1 5.7185373 66.7 1 K.MYLDHDS#VVEYS#EDEIVEADR.I YLR212C 1 1 7.8% 473 52628 4.7 U TUB4 SGDID:S000004202, Chr XII from 566283-564862, reverse complement, Verified ORF, "Gamma-tubulin, involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body" * Mito_110308.27256.27256.3 3.8479 0.3187 100.0 4339.7056 4336.8345 14116.2 1 4.928109 36.8 1 R.S#PY#LPLQPNENEVSGMMLSNMSTVVNVFENACNTFDK.V YIL149C 7 9 7.5% 1679 195140 6.0 U MLP2 SGDID:S000001411, Chr IX from 68067-63028, reverse complement, Verified ORF, "Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved in the Tel1p pathway that controls telomere length" * Mito_110308.9330.9330.3 4.1571 0.3619 100.0 2072.9653 2070.9856 283587.3 1 6.886935 63.0 1 K.TKS#SLYSAQDLLDKHER.K * Mito_110308.9150.9150.3 3.8159 0.3225 100.0 2841.3855 2843.357 108764.9 12 6.5899186 33.3 1 N.EISDLKGKLS#SAENANADLENKFNR.L * Mito_110308.16573.16573.3 3.6367 0.3202 100.0 3185.1555 3184.303 10956.6 1 5.1710954 40.3 1 K.ENAGSLTFLDNKGS#GEDAEEELWNS#PSK.G * Mito_110308.10674.10674.2 5.1501 0.4032 100.0 1816.2927 1814.7115 1713389.8 2 7.0548086 73.9 2 K.GSGEDAEEELWNSPS#K.G * Mito_110308.13504.13504.3 4.1761 0.4269 100.0 2989.3455 2987.269 119868.5 1 8.165093 52.5 1 K.GSGEDAEEELWNS#PSKGNSERPSAVAGF.I * Mito_110308.12754.12754.3 4.2912 0.4591 100.0 3472.9753 3470.5496 9606039.0 2 6.7691236 44.9 2 K.GSGEDAEEELWNS#PSKGNSERPSAVAGFINQK.N * Mito_110308.9961.9961.3 3.5964 0.3604 100.0 4481.9956 4485.1353 7372.7 1 6.4758387 34.1 1 K.NLKPQEQLKNVKNDVS#FNDSQSMVTNKENNIVDSSAAGNK.A YNL188W 1 1 6.2% 433 50653 9.3 U KAR1 SGDID:S000005132, Chr XIV from 286309-287610, Verified ORF, "Essential protein involved in karyogamy during mating and in spindle pole body duplication during mitosis, localizes to the half-bridge of the spindle pole body, interacts with Spc72p during karyogamy, also interacts with Cdc31p" * Mito_110308.17074.17074.3 4.69 0.4099 100.0 3280.7354 3277.4307 94675.6 1 6.6671085 36.9 1 K.AEEYIS#DEDNVKIDEDNIENELQFTPK.I YLR457C 1 1 5.3% 319 37354 10.2 U NBP1 SGDID:S000004449, Chr XII from 1056768-1055809, reverse complement, Verified ORF, "Component of the mitotic apparatus containing a coiled-coil domain, essential for the G2/M transition" * Mito_110308.15039.15039.2 2.9756 0.218 100.0 2026.6527 2024.9575 31859.6 1 5.626281 72.0 1 R.ILEDLLDSS#NIHPSYTK.S YGL219C 1 1 5.2% 459 51990 9.0 U MDM34 SGDID:S000003187, Chr VII from 84256-82877, reverse complement, Verified ORF, "Mitochondrial outer membrane protein, required for normal mitochondrial morphology and inheritance; localizes to dots on the mitochondrial surface near mtDNA nucleoids; interacts genetically with MDM31 and MDM32" * Mito_110308.24006.24006.3 3.4955 0.2497 100.0 2827.6455 2824.1936 16214.2 7 4.7748375 34.4 1 R.MPSLNNYYS#S#QSFPQPKSSTVSSK.Q YDR320C 1 1 5.1% 668 75020 5.3 U SWA2 SGDID:S000002728, Chr IV from 1108096-1106090, reverse complement, Verified ORF, "Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles" * Mito_110308.38281.38281.3 3.1246 0.2707 100.0 3718.6455 3724.5774 2223.8 39 4.7817836 26.8 1 S.KET#TPQSSNS#PSITGSAVADVARTDKSPNDS#LHS.I YFR028C 1 1 4.7% 551 61907 8.0 U CDC14 SGDID:S000001924, Chr VI from 210056-208401, reverse complement, Verified ORF, "Protein phosphatase required for mitotic exit; located in the nucleolus until liberated by the FEAR and Mitotic Exit Network in anaphase, enabling it to act on key substrates to effect a decrease in CDK/B-cyclin activity and mitotic exit" * Mito_110308.8390.8390.3 4.9655 0.4818 100.0 2990.4556 2989.2517 43687.7 16 7.3288693 33.8 1 R.KVVIES#NNSDDESMQDTNGTSNHYPK.V YIL041W 1 1 4.6% 326 36670 5.0 U GVP36 SGDID:S000001303, Chr IX from 276524-277504, Uncharacterized ORF, "Golgi vesicle protein of unknown function; localizes to both early and late Golgi vesicles" * Mito_110308.8178.8178.1 1.8214 0.2401 100.0 1785.97 1785.8993 37296.9 19 4.2443314 50.0 1 S.KKLQEISTS#VSEKTQ.E YGR132C 1 1 4.2% 287 31427 8.2 U PHB1 SGDID:S000003364, Chr VII from 756457-755594, reverse complement, Verified ORF, "Subunit of the prohibitin complex (Phb1p-Phb2p), a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilizes newly synthesized proteins; determinant of replicative life span; involved in mitochondrial segregation" * Mito_110308.28143.28143.2 2.9442 0.2657 100.0 1326.5527 1324.6912 1770.6 1 4.9026527 70.0 1 A.LPIGIIASGIQY#.S YNL009W 1 2 3.3% 420 47856 9.2 U IDP3 SGDID:S000004954, Chr XIV from 614822-616084, Verified ORF, "Peroxisomal NADP-dependent isocitrate dehydrogenase, catalyzes oxidation of isocitrate to alpha-ketoglutarate with the formation of NADP(H+), required for growth on unsaturated fatty acids" * Mito_110308.11200.11200.2 3.6325 0.0524 100.0 1897.4927 1896.8778 58369.6 6 2.9290097 61.9 2 K.QIFDNLFDKEY#KEK.F YHR172W 2 2 3.2% 823 96825 6.4 U SPC97 SGDID:S000001215, Chr VIII from 448335-450806, Verified ORF, "Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque" * Mito_110308.13516.13516.2 4.2027 0.3643 100.0 2259.8928 2258.8843 62396.1 1 6.780888 65.4 1 R.RTNQSS#QEDFNNFMDSMK.N * Mito_110308.16067.16067.3 3.5545 0.4614 100.0 3040.7954 3039.2607 30053.0 1 6.75281 40.3 1 R.TNQSS#QEDFNNFMDSMKNESSLHLR.L YPL109C 1 1 3.2% 657 76176 8.4 U YPL109C SGDID:S000006030, Chr XVI from 347388-345596,345443-345263, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Mito_110308.20054.20054.3 3.1749 0.2972 100.0 2654.8755 2659.272 174086.7 13 4.8634276 33.3 1 I.RQFIIPTSIAFY#LTQNS#FPKQ.N YLR353W 1 1 3.2% 603 66289 6.3 U BUD8 SGDID:S000004345, Chr XII from 834351-836162, Verified ORF, "Protein involved in bud-site selection; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern, and bud at the proximal pole" * Mito_110308.1838.1838.3 3.2509 0.271 100.0 1925.7854 1924.8032 11970.6 30 4.599766 41.2 1 K.SSTSDDSHSPGAPGTS#ARK.I YGL075C 1 3 2.8% 387 44585 8.3 U MPS2 SGDID:S000003043, Chr VII from 368091-366928, reverse complement, Verified ORF, "Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required for insertion of the newly duplicated SPB into the nuclear envelope; potentially phosphorylated by Cdc28p" * Mito_110308.2851.2851.2 3.3191 0.4403 100.0 1182.8328 1182.515 9574.0 1 8.540999 77.8 3 K.NTEGAGIST#PR.K YPL061W 1 1 2.6% 500 54414 5.4 U ALD6 SGDID:S000005982, Chr XVI from 432585-434087, Verified ORF, "Cytosolic aldehyde dehydrogenase that is activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for the conversion of acetaldehyde to acetate; constitutively expressed" * Mito_110308.11912.11912.2 3.0524 0.0884 100.0 1524.3927 1523.6389 149493.8 3 3.2893052 70.0 1 K.SVAVDS#S#ESNLKK.I YMR001C 1 1 2.3% 705 81031 9.0 U CDC5 SGDID:S000004603, Chr XIII from 271136-269019, reverse complement, Verified ORF, "Polo-like kinase with similarity to Xenopus Plx1 and S. pombe Plo1p; found at bud neck, nucleus and SPBs; has multiple functions in mitosis and cytokinesis through phosphorylation of substrates; may be a Cdc28p substrate" * Mito_110308.8526.8526.3 2.7954 0.3212 100.0 1744.4054 1742.92 903540.8 1 6.108603 72.5 1 K.LVHT#PIKGNTADLVGK.E YDL080C 1 1 2.1% 609 68366 6.4 U THI3 SGDID:S000002238, Chr IV from 312471-310642, reverse complement, Verified ORF, "Probable decarboxylase, required for expression of enzymes involved in thiamine biosynthesis; may have a role in catabolism of amino acids to long-chain and complex alcohols" YLR134W 1 1 2.3% 563 61912 6.4 U PDC5 SGDID:S000004124, Chr XII from 410724-412415, Verified ORF, "Minor isoform of pyruvate decarboxylase, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde, regulation is glucose- and ethanol-dependent, repressed by thiamine, involved in amino acid catabolism" YLR044C 1 1 2.3% 563 61495 6.2 U PDC1 SGDID:S000004034, Chr XII from 234082-232391, reverse complement, Verified ORF, "Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde; subject to glucose-, ethanol-, and autoregulation; involved in amino acid catabolism" YGR087C 1 1 2.3% 563 61581 6.2 U PDC6 SGDID:S000003319, Chr VII from 652985-651294, reverse complement, Verified ORF, "Minor isoform of pyruvate decarboxylase, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde, regulation is glucose- and ethanol-dependent, involved in amino acid catabolism" Mito_110308.12351.12351.3 2.674 0.3545 100.0 1581.3555 1580.7006 196143.6 1 5.3173428 64.9 1 H.HT#LGNGDFTVFHR.I YLL003W 1 1 2.0% 946 112978 9.8 U SFI1 SGDID:S000003926, Chr XII from 143200-146040, Verified ORF, "Centrin (Cdc31p)-binding protein required for spindle pole body (SPB) duplication, localizes to the half-bridge of the SPB, required for progression through G(2)-M transition, has similarity to Xenopus laevis XCAP-C" * Mito_110308.15302.15302.2 3.3383 0.2741 100.0 2276.7527 2274.0706 51453.0 10 5.4563956 46.2 1 R.DKFVPETSPT#NIPT#DVLIK.Q Reverse_YPL195W 1 1 1.8% 932 106924 5.1 U APL5 SGDID:S000006116, Chr XVI from 176222-179020, Verified ORF, "Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function" * Mito_110308.1173.1173.2 3.5061 0.0244 99.1 2124.4727 2123.878 15884.4 1 2.868129 55.6 1 K.KDS#SEEENLDLLDKS#NK.T YML031W 1 1 1.7% 655 74134 8.3 U NDC1 SGDID:S000004493, Chr XIII from 214189-216156, Verified ORF, "Nuclear envelope protein with multiple putative transmembrane domains, required for nuclear pore complex assembly and spindle pole body duplication; required for meiosis II" * Mito_110308.8815.8815.2 3.229 0.3437 100.0 1368.7327 1367.6678 416577.6 1 4.9905233 100.0 1 R.VRTDDS#VLLNR.G YNL163C 1 1 1.4% 1110 124464 5.1 U RIA1 SGDID:S000005107, Chr XIV from 330075-326743, reverse complement, Verified ORF, "Cytoplasmic GTPase involved in biogenesis of the 60S ribosome; has similarity to translation elongation factor 2 (Eft1p and Eft2p)" * Mito_110308.10897.10897.2 2.3646 0.2959 100.0 1595.6727 1593.8975 15164.6 22 5.6831746 41.7 1 R.GLAGKVLKS#GTLIEK.G YMR302C 1 1 0.9% 850 96689 8.9 U PRP12 SGDID:S000004917, Chr XIII from 872624-870072, reverse complement, Verified ORF, "Integral inner mitochondrial membrane protein with similarity to exonucleases; prp12 mutants exhibit an increased rate of mt DNA escape" * Mito_110308.2056.2056.2 2.1449 0.2323 100.0 1038.5127 1037.4064 14797.0 11 4.021295 71.4 1 R.T#T#SLNVSR.M Proteins Peptide IDs Spectra Unfiltered 10495 42334 46670 Filtered 36 127 189 Forward matches 35 126 188 Decoy matches 1 1 1 Forward FP rate 2.86 0.79 0.53 Classification Nonredundant Proteins Redundant Proteins Unclassified 0 0