DTASelect v2.0.9 /data/1/catclw/Projects/Jonathan/step_2-5 /scratch/yates/SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta SEQUEST 3.0 in SQT format. true Use criteria 0.0 Minimum peptide confidence 0.05 Peptide false positive rate 0.0 Minimum protein confidence 1.0 Protein false positive rate 1 Minimum charge state 9 Maximum charge state 0.0 Minimum ion proportion 1000 Maximum Sp rank -1.0 Minimum Sp score Include Modified peptide inclusion Any Tryptic status requirement false Multiple, ambiguous IDs allowed Ignore Peptide validation handling XCorr Purge duplicate peptides by protein false Include only loci with unique peptide true Remove subset proteins Ignore Locus validation handling 0 Minimum modified peptides per locus 1000 Minimum redundancy for low coverage loci 2 Minimum peptides per locus Locus Sequence Count Spectrum Count Sequence Coverage Length MolWt pI Validation Status Descriptive Name Unique FileName XCorr DeltCN Conf% M+H+ CalcM+H+ TotalIntensity SpR ZScore IonProportion Redundancy Sequence YKL089W 7 114 19.9% 549 62473 4.8 U MIF2 SGDID:S000001572, Chr XI from 273038-274687, Verified ORF, "Kinetochore protein with homology to human CENP-C, required for structural integrity of the spindle during anaphase spindle elongation, interacts with histones H2A, H2B, and H4, phosphorylated by Ipl1p" * Mif2_02_itms.14255.14255.2 3.0049 0.1907 96.4 2801.7322 2802.0103 7107.7 1 5.063 31.8 1 K.DEYSMENIDDFFKDDETSLISMR.R * Mif2_03_itms.08329.08329.2 2.8915 0.3487 100.0 2132.2722 2133.057 6521.4 8 5.914 47.1 3 K.NVYS*DVPDLVADEDDDDR.V * Mif2_02_itms.06572.06572.2 3.606 0.1804 99.0 2110.4722 2111.2236 9030.5 2 5.349 50.0 2 K.IVTYDESEDEEEILAAQR.R * Mif2_02_itms.04809.04809.2 3.2242 0.1919 97.6 1934.6522 1934.151 4732.4 1 4.529 56.2 2 A.NVKISDTKEETKDEIIA.F * Mif2_02_itms.10947.10947.2 5.0803 0.3789 100.0 1816.8522 1817.0204 6405.3 1 7.899 75.0 24 K.DTKDENFALEIMFDK.H * Mif2_02_itms.12713.12713.2 5.3835 0.1434 100.0 1473.8722 1472.6527 9575.8 1 6.442 86.4 50 K.DENFALEIMFDK.H * Mif2_02_itms.08751.08751.2 4.4969 0.3879 100.0 2075.7522 2075.211 6297.9 1 8.472 58.8 32 K.MFFVQVTVSEDANDDNDK.E contaminant_KERATIN02 3 25 11.4% 622 61987 5.2 U no description * Mif2_03_itms.04687.04687.2 3.6319 0.3269 100.0 1233.7522 1233.2833 6159.8 1 7.74 70.0 9 T.SGGGGGGGLGSGGSIR.S * Mif2_02_itms.08235.08235.2 4.2356 0.4811 100.0 2706.7722 2706.7605 8079.4 1 8.259 37.1 8 R.GGGGSFGYSYGGGSGGGFSASSLGGGFGGGSR.G * Mif2_03_itms.04530.04530.2 3.9099 0.2984 100.0 1792.5521 1792.7324 8414.1 1 7.501 50.0 8 R.GGSGGSYGGGGSGGGYGGGSGSR.G contaminant_KERATIN03 6 25 9.1% 593 59519 5.2 U no description * Mif2_02_itms.06539.06539.3 3.7285 0.2712 95.0 2562.2344 2560.6995 7583.1 1 4.234 29.0 1 S.LRISSSKGS*LGGGFSSGGFSGGSFSR.G * Mif2_02_itms.05636.05636.2 3.2695 0.3775 100.0 2098.1921 2098.193 9897.6 1 7.648 47.7 2 I.SSSKGSLGGGFSSGGFSGGSFSR.G * Mif2_02_itms.06546.06546.2 5.4195 0.3934 100.0 1707.9321 1708.7844 8439.6 1 7.622 72.2 17 K.GSLGGGFSSGGFSGGSFSR.G * Mif2_02_itms.05549.05549.2 2.8067 0.2584 100.0 1451.5521 1451.4949 8865.4 3 6.165 50.0 3 L.GGGFSSGGFSGGSFSR.G * Mif2_02_itms.04973.04973.2 2.9401 0.187 96.3 1382.4922 1382.4668 11635.8 4 4.685 68.2 1 R.ALEESNYELEGK.I * Mif2_02_itms.11610.11610.2 3.4397 0.1599 95.1 1797.4521 1798.0898 9457.6 3 4.716 53.3 1 R.NVQALEIELQSQLALK.Q contaminant_KERATIN21 2 5 8.1% 357 39219 5.2 U no description Mif2_03_itms.11680.11680.2 4.071 0.1456 98.9 1330.4122 1330.5211 6764.3 1 5.495 77.3 3 R.NLDLDSIIAEVK.A * Mif2_02_itms.05078.05078.2 3.8783 0.364 100.0 1448.4321 1448.6163 8286.7 5 6.037 53.1 2 R.AIGGGLSSVGGGSSTIK.Y contaminant_KERATIN22 3 6 7.4% 645 65865 8.0 U no description * Mif2_02_itms.04702.04702.2 3.1672 0.4315 100.0 1255.5721 1255.3298 10754.8 1 6.898 65.4 1 R.GFSSGSAVVSGGSR.R * Mif2_02_itms.07323.07323.2 3.9275 0.4875 100.0 1840.3922 1840.0055 8381.5 1 7.712 45.2 2 K.SISISVAGGGGGFGAAGGFGGR.G Mif2_03_itms.11680.11680.2 4.071 0.1456 98.9 1330.4122 1330.5211 6764.3 1 5.495 77.3 3 R.NLDLDSIIAEVK.A contaminant_KERATIN13 2 7 6.4% 643 65494 6.6 U no description * Mif2_02_itms.05217.05217.2 3.4677 0.1898 100.0 1181.2122 1180.303 8046.1 1 5.615 88.9 4 K.YEELQITAGR.H * Mif2_02_itms.04580.04580.2 4.2982 0.3489 100.0 2385.8123 2385.298 9626.5 1 8.788 36.7 3 R.GGGGGGYGSGGSSYGSGGGSYGSGGGGGGGR.G contaminant_gi|67568|pir||ELPG 3 10 5.6% 266 28821 8.1 U pancreatic elastase (EC 3.4.21.36) I precursor - pig * Mif2_02_itms.14451.14451.2 2.9041 0.3781 100.0 1450.4521 1450.6775 4730.5 5 6.403 58.3 1 R.VSAYISWINNVIA.S * Mif2_02_itms.14610.14610.2 3.1043 0.3338 100.0 1537.2522 1537.7557 6752.6 1 6.844 53.8 3 R.VSAYISWINNVIAS.N * Mif2_02_itms.14589.14589.2 4.0163 0.2256 100.0 1654.6322 1651.8595 8391.6 1 5.044 71.4 6 R.VSAYISWINNVIASN.- contaminant_KERATIN17 2 7 4.4% 590 62461 8.1 U no description * Mif2_02_itms.05182.05182.2 3.5665 0.3369 100.0 1410.8722 1411.5547 6952.5 1 6.573 73.1 4 R.SFSTASAITPSVSR.T Mif2_03_itms.11680.11680.2 4.071 0.1456 98.9 1330.4122 1330.5211 6764.3 1 5.495 77.3 3 R.NLDLDSIIAEVK.A contaminant_KERATIN18 2 7 3.9% 562 59822 8.0 U no description * Mif2_02_itms.05072.05072.2 3.4243 0.4159 100.0 1027.4922 1027.1222 7051.9 1 7.168 88.9 4 R.SGFSSISVSR.S Mif2_03_itms.11680.11680.2 4.071 0.1456 98.9 1330.4122 1330.5211 6764.3 1 5.495 77.3 3 R.NLDLDSIIAEVK.A Proteins Peptide IDs Spectra Unfiltered 10709 67274 75550 Filtered 9 27 197 Forward matches 9 27 197 Decoy matches 0 0 0 Forward FP rate 0.0 0.0 0.0 Classification Nonredundant Proteins Redundant Proteins Unclassified 0 0