DTASelect v2.0.26 /data/1/catclw/Projects/Jamie/Jamie_June2008/Mito_SPB_orange/splitted/phospho_2 /scratch/yates/SGD_S-cerevisiae_na_07-09-2008_reversed.fasta SEQUEST 3.0 in SQT format. --trypstat --cmax 4 --fp 0.2 -p 1 true Use criteria 0.0 Minimum peptide confidence 0.2 Peptide false positive rate 0.0 Minimum protein confidence 1.0 Protein false positive rate 1 Minimum charge state 16 Maximum charge state 0.0 Minimum ion proportion 1000 Maximum Sp rank -1.0 Minimum Sp score Include Modified peptide inclusion Any Tryptic status requirement false Multiple, ambiguous IDs allowed Ignore Peptide validation handling XCorr Purge duplicate peptides by protein false Include only loci with unique peptide true Remove subset proteins Ignore Locus validation handling 0 Minimum modified peptides per locus 1000 Minimum redundancy for low coverage loci 1 Minimum peptides per locus Locus Sequence Count Spectrum Count Sequence Coverage Length MolWt pI Validation Status Descriptive Name Unique FileName XCorr DeltCN Conf% M+H+ CalcM+H+ TotalIntensity SpR ZScore IonProportion Redundancy Sequence YIL002W-A 28 35 94.2% 69 7729 4.7 U YIL002W-A SGDID:S000028835, Chr IX from 350298-350507, Uncharacterized ORF, "Identified by expression profiling and mass spectrometry" * Jamie_Mitco_SPB_53.18839.18839.3 3.3774 0.1822 99.5 2716.5544 2717.1072 8167.7 6 4.085 26.0 1 -.MTRDTPEDVSTAGAKDILDVLNLLK.G * Jamie_Mitco_SPB_10.03393.03393.1 1.8344 0.2602 100.0 1447.54 1448.5297 5451.1 2 5.972 46.2 1 M.TRDTPEDVSTAGAK.D * Jamie_Mitco_SPB_9.02909.02909.2 4.734 0.3176 100.0 1448.9122 1448.5297 6711.0 1 7.866 80.8 3 M.TRDTPEDVSTAGAK.D * Jamie_Mitco_SPB_36.12658.12658.2 3.1921 0.1815 80.0 2118.7522 2117.3176 9622.6 1 3.819 47.4 1 M.TRDTPEDVSTAGAKDILDVL.N * Jamie_Mitco_SPB_53.18867.18867.3 6.8964 0.3885 100.0 2584.0144 2585.9146 8284.3 1 7.728 51.1 1 M.TRDTPEDVSTAGAKDILDVLNLLK.G * Jamie_Mitco_SPB_53.19024.19024.2 4.5899 0.402 100.0 2585.3523 2585.9146 8774.3 1 6.989 47.8 1 M.TRDTPEDVSTAGAKDILDVLNLLK.G * Jamie_Mitco_SPB_54.19091.19091.4 4.3148 0.2038 80.8 2587.1765 2585.9146 7113.0 1 4.452 39.9 1 M.TRDTPEDVSTAGAKDILDVLNLLK.G * Jamie_Mitco_SPB_50.17711.17711.3 6.293 0.4772 100.0 3084.4744 3086.4233 10773.4 1 9.956 39.3 1 M.TRDTPEDVSTAGAKDILDVLNLLKGGEEK.I * Jamie_Mitco_SPB_50.17919.17919.4 6.5898 0.4438 100.0 3088.4565 3086.4233 9180.9 1 7.472 31.5 1 M.TRDTPEDVSTAGAKDILDVLNLLKGGEEK.I * Jamie_Mitco_SPB_57.20301.20301.4 7.8173 0.497 100.0 3887.8965 3885.3582 10766.9 1 8.084 30.0 1 M.TRDTPEDVSTAGAKDILDVLNLLKGGEEKISEVELK.L * Jamie_Mitco_SPB_67.24107.24107.4 4.2693 0.2587 92.9 4634.4966 4631.2036 13363.2 1 4.288 18.3 1 M.TRDTPEDVSTAGAKDILDVLNLLKGGEEKISEVELKLDEMEK.K * Jamie_Mitco_SPB_69.24573.24573.4 5.4715 0.4653 100.0 4372.5767 4373.911 6557.5 1 6.784 23.1 1 R.DTPEDVSTAGAKDILDVLNLLKGGEEKISEVELKLDEMEK.K * Jamie_Mitco_SPB_46.16457.16457.1 2.2848 0.1678 80.0 1413.68 1412.7129 6209.8 1 4.255 58.3 1 A.GAKDILDVLNLLK.G * Jamie_Mitco_SPB_49.17499.17499.2 5.1269 0.3911 100.0 1656.9722 1656.917 9963.2 1 7.736 82.1 1 K.DILDVLNLLKGGEEK.I * Jamie_Mitco_SPB_49.17496.17496.3 2.7839 0.281 100.0 1659.1444 1656.917 2714.8 4 5.017 46.4 1 K.DILDVLNLLKGGEEK.I * Jamie_Mitco_SPB_62.22127.22127.3 3.8133 0.2607 100.0 3329.6343 3329.8713 8960.0 1 4.975 24.1 1 K.DILDVLNLLKGGEEKISEVELKLDEMEKK.M * Jamie_Mitco_SPB_69.24537.24537.4 3.8771 0.2214 81.5 3087.2166 3086.6086 9161.4 1 3.893 24.4 1 D.ILDVLNLLKGGEEKISEVELKLDEMEK.K * Jamie_Mitco_SPB_26.09318.09318.2 1.9402 0.1206 97.5 2062.912 2064.3125 8942.6 140 3.823 29.4 1 K.GGEEKISEVELKLDEMEK.K * Jamie_Mitco_SPB_23.08217.08217.3 4.1368 0.1781 100.0 2064.1743 2064.3125 10036.9 2 4.89 39.7 1 K.GGEEKISEVELKLDEMEK.K * Jamie_Mitco_SPB_22.07858.07858.4 3.9083 0.261 100.0 2194.8564 2192.4866 9197.0 3 4.59 32.4 2 K.GGEEKISEVELKLDEMEKK.M * Jamie_Mitco_SPB_39.13693.13693.4 4.7309 0.2853 100.0 2637.4565 2639.0054 7973.8 21 5.443 27.3 1 K.GGEEKISEVELKLDEMEKKMDSL.L * Jamie_Mitco_SPB_55.19511.19511.4 4.1958 0.0726 86.7 4516.2163 4514.0767 8257.8 417 3.547 15.8 1 K.GGEEKISEVELKLDEMEKKMDSLLVQLEDLHRDNNDLAK.S * Jamie_Mitco_SPB_20.06879.06879.2 4.5714 0.4332 100.0 1563.1122 1563.8036 8036.8 1 7.726 87.5 5 K.ISEVELKLDEMEK.K * Jamie_Mitco_SPB_53.18922.18922.3 2.8807 0.1138 93.7 4010.4543 4013.5679 9547.3 35 3.736 18.9 1 K.ISEVELKLDEMEKKMDSLLVQLEDLHRDNNDLAK.S * Jamie_Mitco_SPB_53.18900.18900.4 4.5124 0.1125 95.1 4017.0566 4013.5679 11190.1 1 3.502 22.7 1 K.ISEVELKLDEMEKKMDSLLVQLEDLHRDNNDLAK.S * Jamie_Mitco_SPB_29.10335.10335.2 4.4132 0.3773 100.0 2467.4321 2468.7876 6546.1 1 7.11 55.0 1 K.KMDSLLVQLEDLHRDNNDLAK.S * Jamie_Mitco_SPB_31.11095.11095.2 3.0844 0.2901 100.0 2340.2122 2340.6135 8685.5 1 5.952 39.5 1 K.MDSLLVQLEDLHRDNNDLAK.S * Jamie_Mitco_SPB_32.11254.11254.3 3.6344 0.2896 100.0 2429.7244 2427.6917 6640.3 1 4.831 41.2 1 K.MDSLLVQLEDLHRDNNDLAKS.S YOR257W 15 16 82.6% 161 18751 4.6 U CDC31 SGDID:S000005783, Chr XV from 811006-811491, Verified ORF, "Component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; interacts with Kar1p" * Jamie_Mitco_SPB_21.07498.07498.2 3.3109 0.4131 100.0 1860.2522 1860.0282 5168.2 1 7.531 59.4 1 R.SSLQSGPLNSELLEEQK.Q * Jamie_Mitco_SPB_30.10674.10674.2 2.0399 0.267 90.9 2592.7122 2593.804 8235.7 10 4.44 29.5 1 R.SSLQSGPLNSELLEEQKQEIYEA.F * Jamie_Mitco_SPB_61.21621.21621.3 4.7226 0.4749 100.0 4781.304 4781.215 8493.8 1 7.924 21.2 1 R.SSLQSGPLNSELLEEQKQEIYEAFSLFDMNNDGFLDYHELK.V * Jamie_Mitco_SPB_51.18259.18259.2 2.8018 0.3897 100.0 2939.5122 2940.21 6143.0 1 6.316 32.6 1 K.QEIYEAFSLFDMNNDGFLDYHELK.V * Jamie_Mitco_SPB_65.23295.23295.4 6.9859 0.4214 100.0 4552.5767 4551.1855 10469.3 1 6.972 24.3 1 K.ALGFELPKREILDLIDEYDSEGRHLMKYDDFYIVMGEK.I * Jamie_Mitco_SPB_32.11241.11241.2 4.0331 0.5357 100.0 1822.4922 1823.9542 6767.8 1 8.763 85.7 1 K.REILDLIDEYDSEGR.H * Jamie_Mitco_SPB_46.16207.16207.2 1.7926 0.0275 87.8 1665.3922 1667.7667 9034.9 19 3.323 38.5 1 R.EILDLIDEYDSEGR.H * Jamie_Mitco_SPB_37.13199.13199.1 1.5535 0.2798 100.0 1666.69 1667.7667 2008.1 1 5.276 46.2 1 R.EILDLIDEYDSEGR.H * Jamie_Mitco_SPB_31.11086.11086.3 2.8736 0.127 95.8 1892.2144 1890.222 6110.1 43 4.797 33.9 1 R.HLMKYDDFYIVMGEK.I * Jamie_Mitco_SPB_28.09927.09927.2 3.1899 0.4251 100.0 1380.0322 1380.5549 9663.5 1 7.577 90.0 2 K.YDDFYIVMGEK.I * Jamie_Mitco_SPB_28.09936.09936.1 2.4472 0.269 100.0 1381.18 1380.5549 8672.5 1 5.062 65.0 1 K.YDDFYIVMGEK.I * Jamie_Mitco_SPB_18.06412.06412.2 1.7774 0.0171 87.9 1394.6522 1394.4833 5433.4 84 3.544 50.0 1 R.AFQLFDDDHTGK.I * Jamie_Mitco_SPB_18.06343.06343.2 3.9813 0.2434 100.0 1861.0922 1860.0746 6162.9 1 5.877 83.3 1 R.RVAKELGETLTDEELR.A * Jamie_Mitco_SPB_102.36723.36723.3 2.652 0.0841 90.1 4790.304 4790.1406 10410.6 3 4.308 17.1 1 R.RVAKELGETLTDEELRAMIEEFDLDGDGEINENEFIAICTDS.- * Jamie_Mitco_SPB_72.25824.25824.3 3.0053 0.2186 99.5 4634.3945 4633.953 7748.7 2 4.251 16.9 1 R.VAKELGETLTDEELRAMIEEFDLDGDGEINENEFIAICTDS.- YKL042W 65 76 81.3% 363 42271 7.9 U SPC42 SGDID:S000001525, Chr XI from 358119-359210, Verified ORF, "Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane" * Jamie_Mitco_SPB_30.10800.10800.2 2.7715 0.2766 100.0 2529.2722 2528.7546 4155.8 3 5.046 39.5 1 R.LYDDYYNIPYQYSNPTPMNR.D * Jamie_Mitco_SPB_35.12257.12257.2 2.7738 0.0246 97.9 2610.112 2608.7546 5781.7 3 3.351 39.5 1 R.LYDDYYNIPYQY*SNPTPMNR.D * Jamie_Mitco_SPB_32.11375.11375.3 2.5941 0.1536 93.8 3514.3743 3515.6619 8975.3 18 3.498 20.4 1 R.LYDDYYNIPYQYS*NPTPMNRDYNDVGSR.I * Jamie_Mitco_SPB_29.10392.10392.2 2.1519 0.2505 91.1 2488.4722 2488.6936 7610.6 1 5.161 37.5 1 N.IPYQYSNPTPMNRDYNDVGSR.I * Jamie_Mitco_SPB_16.05446.05446.3 2.9703 0.2357 100.0 1576.4944 1575.8052 5496.6 1 4.375 41.7 1 R.INADKLVPEEYKR.N * Jamie_Mitco_SPB_15.05195.05195.2 4.1748 0.2294 100.0 1577.1122 1575.8052 4877.8 1 4.901 79.2 1 R.INADKLVPEEYKR.N * Jamie_Mitco_SPB_19.06586.06586.3 3.7637 0.254 100.0 2502.5044 2502.7434 4092.4 14 5.255 30.3 1 R.INADKLVPEEYKRNT*EFINK.A * Jamie_Mitco_SPB_11.03960.03960.1 2.1005 0.0161 97.2 865.98 865.96155 3985.5 38 3.175 75.0 1 R.NTEFINK.A * Jamie_Mitco_SPB_13.04637.04637.2 4.21 0.3507 100.0 1534.2922 1534.7123 4964.9 1 6.798 87.5 1 R.NTEFINKAVQQNK.E * Jamie_Mitco_SPB_12.04323.04323.2 2.4396 0.1549 100.0 1318.0922 1319.5033 7922.3 1 5.317 65.0 1 K.AVQQNKELNFK.L * Jamie_Mitco_SPB_14.05004.05004.2 2.8688 0.3273 100.0 1589.0521 1588.8502 5327.7 2 5.239 58.3 1 K.AVQQNKELNFKLR.E * Jamie_Mitco_SPB_14.04923.04923.2 4.0986 0.3218 100.0 1405.5922 1406.622 3282.5 1 5.817 80.0 1 R.EKQNEIFELKK.I * Jamie_Mitco_SPB_15.05281.05281.1 2.2137 0.2388 100.0 1323.53 1324.5602 5440.0 6 4.886 55.0 1 R.SKLEKYVDITK.K * Jamie_Mitco_SPB_11.03780.03780.1 1.723 0.0067 95.5 738.14 738.85895 5437.9 8 3.964 80.0 1 K.YVDITK.K * Jamie_Mitco_SPB_26.09100.09100.2 3.5473 0.3568 100.0 2175.3123 2176.518 7809.4 1 5.67 47.1 1 K.YVDITKKLEDQNLNLQIK.I * Jamie_Mitco_SPB_26.09129.09129.3 3.25 0.2507 100.0 2175.9543 2176.518 6761.4 4 4.757 33.8 1 K.YVDITKKLEDQNLNLQIK.I * Jamie_Mitco_SPB_17.06106.06106.2 2.3149 0.0373 97.5 1329.9122 1328.5083 6351.7 13 3.221 65.0 1 K.LEDQNLNLQIK.I * Jamie_Mitco_SPB_11.03864.03864.1 1.515 0.2239 100.0 1110.25 1111.2401 4177.7 9 4.818 55.6 1 K.KLSDANSTFK.E * Jamie_Mitco_SPB_11.03838.03838.2 2.717 0.172 100.0 1112.8121 1111.2401 5409.2 71 4.03 55.6 1 K.KLSDANSTFK.E * Jamie_Mitco_SPB_14.04695.04695.2 2.8486 0.2032 100.0 1478.9521 1479.5615 4853.8 2 4.948 72.7 1 K.LSDANST*FKEMR.F * Jamie_Mitco_SPB_24.08423.08423.2 5.214 0.424 100.0 2080.9722 2080.2275 8297.9 1 7.605 64.7 1 K.VKDPMVDDDPVSENYDQI.N * Jamie_Mitco_SPB_22.07761.07761.3 5.5044 0.4045 100.0 2518.0144 2518.7546 6603.8 1 8.054 44.0 1 K.VKDPMVDDDPVSENYDQINVPK.H * Jamie_Mitco_SPB_23.08031.08031.2 2.0903 0.0047 89.6 2520.4922 2518.7546 6377.6 6 3.047 33.3 1 K.VKDPMVDDDPVSENYDQINVPK.H * Jamie_Mitco_SPB_23.08225.08225.2 2.1734 0.2518 100.0 2598.612 2598.7546 4635.2 11 4.673 38.1 1 K.VKDPMVDDDPVS*ENYDQINVPK.H * Jamie_Mitco_SPB_17.06093.06093.4 3.9371 0.203 100.0 2811.4565 2812.0833 6511.4 13 4.743 24.6 1 K.VKDPMVDDDPVSENYDQINVPKHR.A * Jamie_Mitco_SPB_18.06316.06316.4 4.3448 0.2991 100.0 2892.0166 2892.0833 6085.9 1 5.618 34.1 1 K.VKDPMVDDDPVS*ENYDQINVPKHR.A * Jamie_Mitco_SPB_18.06241.06241.4 4.2817 0.1958 100.0 3692.4165 3692.0112 7401.7 1 4.298 23.4 1 K.VKDPMVDDDPVSENYDQINVPKHRAPDATGNPR.T * Jamie_Mitco_SPB_1.00049.00049.2 3.1909 0.3507 100.0 1192.2722 1192.2797 4753.3 1 7.83 75.0 3 K.HRAPDATGNPR.T * Jamie_Mitco_SPB_1.00310.00310.2 3.7594 0.4619 100.0 1552.7522 1553.5828 6918.5 1 8.033 80.8 1 R.TTNKVSNTSDQDSR.L * Jamie_Mitco_SPB_27.09384.09384.1 1.2214 0.0118 84.7 1296.09 1297.5554 4848.9 11 3.062 45.0 1 R.TLSVLTNYVMR.S * Jamie_Mitco_SPB_27.09477.09477.2 3.0059 0.1283 100.0 1300.1522 1297.5554 8854.6 1 3.941 85.0 3 R.TLSVLTNYVMR.S * Jamie_Mitco_SPB_64.22788.22788.2 2.8258 0.2472 97.1 2541.0723 2541.663 7416.8 3 4.664 31.0 1 R.SEDGNNDRMS*PLPS*PLNTILPI.N * Jamie_Mitco_SPB_35.12599.12599.3 3.432 0.2489 100.0 2766.2344 2766.058 9413.4 66 4.427 25.0 1 R.SEDGNNDRMSPLPSPLNTILPINNR.L * Jamie_Mitco_SPB_40.14164.14164.3 4.2909 0.3179 100.0 2846.1243 2846.058 7072.3 1 5.195 33.3 1 R.SEDGNNDRMS*PLPSPLNTILPINNR.L * Jamie_Mitco_SPB_49.17356.17356.3 3.8335 0.147 99.5 2927.8145 2926.058 7713.6 8 4.408 25.0 2 R.SEDGNNDRMS*PLPS*PLNTILPINNR.L * Jamie_Mitco_SPB_40.14113.14113.2 2.1138 0.0493 93.9 1876.5922 1878.2402 8397.2 10 3.925 43.8 1 R.MSPLPSPLNTILPINNR.L * Jamie_Mitco_SPB_49.17565.17565.2 2.5972 0.0921 99.4 1959.2522 1958.2402 10395.4 22 3.645 37.5 2 R.MSPLPSPLNT*ILPINNR.L * Jamie_Mitco_SPB_55.19477.19477.2 3.0075 0.3732 100.0 2037.2322 2038.2402 7765.7 251 6.039 34.4 3 R.MS*PLPS*PLNTILPINNR.L * Jamie_Mitco_SPB_27.09467.09467.2 3.5828 0.0766 100.0 1959.7322 1958.1624 4715.0 1 5.035 65.6 1 K.VNPSDDDIMMYESAELK.R * Jamie_Mitco_SPB_22.07829.07829.2 2.2326 0.1216 98.9 2113.0923 2114.3499 6399.4 17 4.317 35.3 1 K.VNPSDDDIMMYESAELKR.V * Jamie_Mitco_SPB_31.10845.10845.2 2.847 0.1156 83.2 2088.152 2088.3225 6450.1 3 4.223 43.8 1 Y.ESAELKRVEEEIEELKR.K * Jamie_Mitco_SPB_30.10546.10546.3 4.4024 0.1595 87.0 2218.8542 2216.4966 4052.0 1 4.32 47.1 1 Y.ESAELKRVEEEIEELKRK.I * Jamie_Mitco_SPB_23.07968.07968.3 3.9776 0.403 100.0 1799.1843 1801.05 8517.2 1 6.366 51.9 1 A.ELKRVEEEIEELKR.K * Jamie_Mitco_SPB_22.07816.07816.4 3.0753 0.2513 91.3 1804.2565 1801.05 7090.1 1 4.688 38.5 1 A.ELKRVEEEIEELKR.K * Jamie_Mitco_SPB_14.04984.04984.3 2.6279 0.2078 99.5 1427.7244 1430.6011 4453.4 12 4.227 45.0 1 K.RVEEEIEELKR.K * Jamie_Mitco_SPB_14.04761.04761.1 2.5173 0.2085 100.0 1274.32 1274.4136 6159.9 1 5.163 66.7 1 R.VEEEIEELKR.K * Jamie_Mitco_SPB_13.04599.04599.2 3.0784 0.0792 100.0 1401.3522 1402.5876 4838.7 1 4.503 80.0 1 R.VEEEIEELKRK.I * Jamie_Mitco_SPB_46.16325.16325.2 3.0253 0.1812 100.0 2617.8323 2615.847 3988.7 1 4.215 45.2 2 R.KLS*LNNQLQELQSMMDGDDNIK.L * Jamie_Mitco_SPB_40.14332.14332.2 3.2778 0.4426 100.0 3191.412 3192.5837 5858.7 1 8.113 35.2 1 R.KLSLNNQLQELQSMMDGDDNIKLDNVSK.H * Jamie_Mitco_SPB_44.15723.15723.3 6.0192 0.4197 100.0 3273.2043 3272.5837 6100.4 1 6.916 33.3 2 R.KLS*LNNQLQELQSMMDGDDNIKLDNVSK.H * Jamie_Mitco_SPB_47.16907.16907.2 2.4593 0.1862 100.0 2409.2322 2407.6729 8222.5 1 4.226 32.5 1 K.LSLNNQLQELQSMMDGDDNIK.L * Jamie_Mitco_SPB_45.16029.16029.2 3.3844 0.3665 100.0 3063.5122 3064.4097 6237.5 1 6.372 28.8 1 K.LSLNNQLQELQSMMDGDDNIKLDNVSK.H * Jamie_Mitco_SPB_45.16017.16017.3 4.4668 0.3159 100.0 3065.7244 3064.4097 10539.8 1 5.687 31.7 1 K.LSLNNQLQELQSMMDGDDNIKLDNVSK.H * Jamie_Mitco_SPB_121.43515.43515.3 3.4733 0.05 93.2 3103.1343 3104.0266 6541.5 107 4.535 24.0 1 R.HSSQS*SRDYSPSSDACLECSNDLYEK.N * Jamie_Mitco_SPB_122.43635.43635.4 3.712 0.2561 100.0 3375.2166 3374.3179 6730.4 11 4.891 22.8 1 R.HSSQSSRDY*SPSSDACLECSNDLYEKNR.V * Jamie_Mitco_SPB_19.06633.06633.2 5.0461 0.3593 100.0 2256.4922 2254.2546 5649.9 1 6.616 66.7 1 R.DYSPSSDACLECSNDLYEK.N * Jamie_Mitco_SPB_24.08373.08373.2 2.0797 0.2449 100.0 2333.5522 2334.2546 6621.1 6 4.584 33.3 1 R.DY*SPSSDACLECSNDLYEK.N * Jamie_Mitco_SPB_22.07785.07785.2 3.9904 0.4429 100.0 2333.6921 2334.2546 7604.9 1 7.145 58.3 2 R.DYS*PSSDACLECSNDLYEK.N * Jamie_Mitco_SPB_17.05871.05871.3 3.038 0.2216 100.0 2524.5842 2524.546 5153.4 2 4.48 31.2 1 R.DYSPSSDACLECSNDLYEKNR.V * Jamie_Mitco_SPB_14.04794.04794.3 3.4457 0.1434 99.0 2501.8442 2498.6465 5668.1 256 4.176 27.5 1 K.NRVKPENNMSETFATPT*PNNR.- * Jamie_Mitco_SPB_16.05565.05565.2 2.8191 0.3661 100.0 1956.3322 1958.0637 6710.9 1 6.702 56.2 1 R.VKPENNMSETFAT*PTPN.N * Jamie_Mitco_SPB_16.05484.05484.2 1.9604 0.0699 93.4 2227.112 2228.355 6230.3 166 3.511 30.6 1 R.VKPENNMSET*FATPTPNNR.- * Jamie_Mitco_SPB_14.04908.04908.2 3.9245 0.535 100.0 2228.5122 2228.355 4270.9 2 7.364 63.9 1 R.VKPENNMSETFAT*PTPNNR.- * Jamie_Mitco_SPB_15.05049.05049.3 4.5789 0.3597 100.0 2229.0544 2228.355 6330.8 1 6.279 43.1 1 R.VKPENNMSETFAT*PTPNNR.- * Jamie_Mitco_SPB_15.05235.05235.3 3.3185 0.29 100.0 2307.3542 2308.355 4614.2 12 4.746 30.6 1 R.VKPENNMSET*FAT*PTPNNR.- YIL149C 290 378 78.0% 1679 195140 6.0 U MLP2 SGDID:S000001411, Chr IX from 68067-63028, reverse complement, Verified ORF, "Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved in the Tel1p pathway that controls telomere length" * Jamie_Mitco_SPB_41.14615.14615.3 3.638 0.1864 99.4 3374.2144 3375.8035 7364.0 3 4.79 24.1 1 K.IAKFERSEEEVTKLNVLVDEIKSQYYSR.I * Jamie_Mitco_SPB_10.03439.03439.2 1.6419 0.1206 96.2 1252.7722 1254.3391 5219.9 29 3.813 66.7 1 K.FERSEEEVTK.L * Jamie_Mitco_SPB_39.13907.13907.4 4.4184 0.1934 100.0 3066.9766 3063.391 9351.4 13 4.331 22.9 1 K.FERSEEEVTKLNVLVDEIKSQYYSR.I * Jamie_Mitco_SPB_29.10203.10203.2 4.5651 0.3834 100.0 1846.0922 1846.085 8097.8 1 7.649 73.3 1 R.SEEEVTKLNVLVDEIK.S * Jamie_Mitco_SPB_39.13828.13828.3 7.2322 0.3997 100.0 2631.6843 2630.9116 6782.1 1 7.36 52.4 1 R.SEEEVTKLNVLVDEIKSQYYSR.I * Jamie_Mitco_SPB_25.08790.08790.2 4.1061 0.3373 100.0 1716.0721 1714.9158 8906.7 1 7.406 80.8 1 L.NVLVDEIKSQYYSR.I * Jamie_Mitco_SPB_35.12329.12329.4 4.2787 0.0588 84.4 3831.8564 3830.2415 7628.5 24 3.742 20.8 1 R.ISKLKQLLDESSEQKNTAKEELNGLKDQLNEER.S * Jamie_Mitco_SPB_26.09159.09159.4 6.5381 0.3915 100.0 3500.3364 3501.8296 6028.5 1 7.394 28.7 5 K.LKQLLDESSEQKNTAKEELNGLKDQLNEER.S * Jamie_Mitco_SPB_18.06299.06299.3 4.5025 0.2402 100.0 3503.1543 3501.8296 5774.8 1 6.035 27.6 3 K.LKQLLDESSEQKNTAKEELNGLKDQLNEER.S * Jamie_Mitco_SPB_24.08625.08625.4 4.1515 0.2049 100.0 3259.7766 3260.496 7659.2 4 5.124 26.5 1 K.QLLDESSEQKNTAKEELNGLKDQLNEER.S * Jamie_Mitco_SPB_24.08523.08523.4 3.5807 0.103 86.6 3506.4966 3503.7617 6055.9 2 3.635 21.8 1 K.QLLDESSEQKNTAKEELNGLKDQLNEERSR.Y * Jamie_Mitco_SPB_19.06652.06652.2 4.6659 0.3892 100.0 2101.632 2102.2656 6794.1 1 7.602 58.8 1 K.NTAKEELNGLKDQLNEER.S * Jamie_Mitco_SPB_20.06891.06891.3 3.1049 0.1476 98.1 2101.7944 2102.2656 8302.1 2 4.251 36.8 1 K.NTAKEELNGLKDQLNEER.S * Jamie_Mitco_SPB_8.02686.02686.1 1.7964 0.0191 96.3 815.76 816.97314 3038.1 7 3.932 75.0 1 R.EIDALKK.Q * Jamie_Mitco_SPB_13.04384.04384.2 2.8855 0.2293 100.0 1337.3922 1336.5547 6530.6 1 5.544 75.0 1 K.KQLHVSHEAMR.E * Jamie_Mitco_SPB_1.00277.00277.2 2.1946 0.2614 93.8 1600.5122 1601.7344 5842.7 2 4.022 62.5 1 V.SHEAMREVNDEKR.V * Jamie_Mitco_SPB_20.06855.06855.3 3.0478 0.1632 99.0 2321.8442 2324.512 8157.0 1 4.647 36.8 1 R.EVNDEKRVKEEYDIWQSR.D * Jamie_Mitco_SPB_23.08109.08109.4 6.4054 0.3103 100.0 4123.5366 4125.325 9698.4 1 5.967 21.2 1 R.EVNDEKRVKEEYDIWQSRDQGNDSLNDDLNKENK.L * Jamie_Mitco_SPB_17.06021.06021.2 3.2695 0.397 100.0 1608.2922 1609.7819 7838.3 2 6.271 54.5 1 K.RVKEEYDIWQSR.D * Jamie_Mitco_SPB_20.07015.07015.4 3.7811 0.0581 82.0 3412.6565 3410.595 9379.7 177 3.76 21.0 1 K.RVKEEYDIWQSRDQGNDSLNDDLNKENK.L * Jamie_Mitco_SPB_17.05892.05892.1 2.2693 0.3901 100.0 1452.42 1453.5944 4115.1 1 6.76 65.0 1 R.VKEEYDIWQSR.D * Jamie_Mitco_SPB_17.05863.05863.2 3.2949 0.1041 100.0 1455.0721 1453.5944 5889.0 1 4.444 80.0 1 R.VKEEYDIWQSR.D * Jamie_Mitco_SPB_19.06615.06615.4 5.6846 0.4128 100.0 3253.8965 3254.4075 4641.2 1 7.114 30.8 1 R.VKEEYDIWQSRDQGNDSLNDDLNKENK.L * Jamie_Mitco_SPB_26.09201.09201.3 6.5625 0.4723 100.0 3636.7444 3636.9138 9629.5 1 8.525 31.9 1 R.VKEEYDIWQSRDQGNDSLNDDLNKENKLLR.R * Jamie_Mitco_SPB_20.06916.06916.3 4.1523 0.338 100.0 3025.9143 3027.1008 9110.4 7 6.367 24.0 1 K.EEYDIWQSRDQGNDSLNDDLNKENK.L * Jamie_Mitco_SPB_19.06701.06701.2 2.9589 0.2108 100.0 1819.3722 1819.8363 6740.8 15 4.436 43.3 1 R.DQGNDSLNDDLNKENK.L * Jamie_Mitco_SPB_18.06207.06207.3 3.5904 0.2365 100.0 2201.9043 2202.3428 4009.5 1 4.863 40.3 1 R.DQGNDSLNDDLNKENKLLR.R * Jamie_Mitco_SPB_18.06220.06220.2 3.6448 0.2653 100.0 2202.872 2202.3428 6038.9 1 4.949 58.3 1 R.DQGNDSLNDDLNKENKLLR.R * Jamie_Mitco_SPB_11.03617.03617.1 1.1484 0.0252 85.0 920.77 921.0355 5846.9 439 3.427 50.0 1 K.TLTEEVTK.S * Jamie_Mitco_SPB_15.05391.05391.2 3.615 0.4266 100.0 2072.412 2074.2493 6717.5 1 8.751 55.9 1 K.TLTEEVTKSSHVENLEEK.L * Jamie_Mitco_SPB_43.15275.15275.3 3.9618 0.3954 100.0 4072.1643 4071.4585 11065.1 1 5.73 19.7 1 K.TLTEEVTKSSHVENLEEKLYQMQSNYESVFTYNK.F * Jamie_Mitco_SPB_43.15240.15240.4 3.8958 0.1533 95.8 4072.6165 4071.4585 9572.3 1 4.347 20.2 1 K.TLTEEVTKSSHVENLEEKLYQMQSNYESVFTYNK.F * Jamie_Mitco_SPB_41.14685.14685.2 3.9151 0.433 100.0 3169.0322 3169.4463 7794.3 1 7.847 36.0 1 K.SSHVENLEEKLYQMQSNYESVFTYNK.F * Jamie_Mitco_SPB_28.09741.09741.2 2.698 0.319 100.0 2014.3522 2016.2324 10143.1 1 5.529 43.3 1 K.LYQMQSNYESVFTYNK.F * Jamie_Mitco_SPB_16.05681.05681.2 2.4024 0.0478 97.2 1905.0122 1906.146 6455.5 15 3.705 40.0 1 K.FLLNQNKQLSQSVEEK.V * Jamie_Mitco_SPB_30.10594.10594.3 2.888 0.1456 95.8 2508.9243 2506.9202 7572.3 22 3.94 32.5 1 K.FLLNQNKQLSQSVEEKVLEMK.N * Jamie_Mitco_SPB_19.06587.06587.3 3.8722 0.4042 100.0 2270.4243 2268.6306 7666.5 1 5.963 32.9 1 K.VLEMKNLKDTASVEKAEFSK.E * Jamie_Mitco_SPB_9.03033.03033.2 2.2927 0.2359 100.0 1104.2522 1105.2334 3276.2 1 5.754 83.3 1 K.NLKDTASVEK.A * Jamie_Mitco_SPB_19.06825.06825.2 3.0769 0.3438 100.0 2399.412 2398.734 6184.0 1 5.737 40.0 1 K.NLKDTASVEKAEFSKEMTLQK.N * Jamie_Mitco_SPB_15.05314.05314.1 2.1277 0.256 100.0 1536.39 1537.6764 3757.8 1 5.105 62.5 1 R.SQLTSLEKDCSLR.A * Jamie_Mitco_SPB_17.05805.05805.2 3.6323 0.2215 100.0 1539.3121 1537.6764 7863.8 1 5.418 70.8 2 R.SQLTSLEKDCSLR.A * Jamie_Mitco_SPB_33.11721.11721.4 4.22 0.2624 100.0 4562.0166 4561.847 7024.8 3 4.994 18.9 1 R.SQLTSLEKDCSLRAIEKNDDNSCRNPEHTDVIDELIDTK.L * Jamie_Mitco_SPB_27.09447.09447.4 4.5295 0.2733 100.0 3676.6565 3674.8464 7741.3 1 4.882 25.6 1 K.DCSLRAIEKNDDNSCRNPEHTDVIDELIDTK.L * Jamie_Mitco_SPB_23.08049.08049.3 4.7346 0.2671 100.0 3045.0544 3043.1938 9132.1 2 5.609 28.0 2 R.AIEKNDDNSCRNPEHTDVIDELIDTK.L * Jamie_Mitco_SPB_26.09193.09193.3 4.2953 0.3598 100.0 3310.1643 3312.5408 8521.1 1 6.17 25.9 1 R.AIEKNDDNSCRNPEHTDVIDELIDTKLR.L * Jamie_Mitco_SPB_26.09203.09203.4 6.9373 0.3784 100.0 3314.6565 3312.5408 8814.9 1 6.251 29.6 1 R.AIEKNDDNSCRNPEHTDVIDELIDTKLR.L * Jamie_Mitco_SPB_30.10677.10677.4 5.9212 0.4154 100.0 3683.2166 3682.9897 9330.8 1 6.265 25.6 3 R.AIEKNDDNSCRNPEHTDVIDELIDTKLRLEK.S * Jamie_Mitco_SPB_25.08973.08973.3 4.0777 0.2768 100.0 2603.2144 2601.666 7516.6 1 5.497 38.1 3 K.NDDNSCRNPEHTDVIDELIDTK.L * Jamie_Mitco_SPB_28.09886.09886.2 2.4654 0.2545 100.0 1739.7522 1739.8767 7822.2 1 5.138 50.0 1 R.NPEHTDVIDELIDTK.L * Jamie_Mitco_SPB_60.21567.21567.3 4.1682 0.2123 94.7 3070.5544 3069.4846 8769.3 5 5.157 27.1 1 R.NQKFQLQNQLEDFILELEHKTPELI.S * Jamie_Mitco_SPB_58.20683.20683.3 6.6767 0.4992 100.0 3433.6443 3431.9133 7719.7 1 8.23 35.2 1 R.NQKFQLQNQLEDFILELEHKTPELISFK.E * Jamie_Mitco_SPB_56.19921.19921.3 3.4658 0.0070 87.6 3717.6543 3717.2163 8462.9 26 3.302 20.7 1 R.NQKFQLQNQLEDFILELEHKTPELISFKER.T * Jamie_Mitco_SPB_55.19491.19491.4 6.8188 0.4595 100.0 3717.8965 3717.2163 9403.6 1 7.626 28.2 1 R.NQKFQLQNQLEDFILELEHKTPELISFKER.T * Jamie_Mitco_SPB_54.19273.19273.2 3.6688 0.4405 100.0 2143.8123 2145.4197 6410.6 1 7.752 59.4 1 K.FQLQNQLEDFILELEHK.T * Jamie_Mitco_SPB_54.19321.19321.3 3.848 0.0895 98.1 2148.6543 2145.4197 3562.7 1 3.897 43.8 1 K.FQLQNQLEDFILELEHK.T * Jamie_Mitco_SPB_57.20328.20328.3 5.9195 0.3656 100.0 3347.6943 3346.8076 8241.9 1 6.245 33.7 1 K.FQLQNQLEDFILELEHKTPELISFKER.T * Jamie_Mitco_SPB_16.05608.05608.2 2.453 0.3535 100.0 1219.1522 1220.4111 4729.3 10 5.531 72.2 1 K.TPELISFKER.T * Jamie_Mitco_SPB_13.04396.04396.3 1.5559 0.1671 87.8 1238.8444 1241.4331 4033.3 311 3.477 38.9 1 R.TKSLEHELKR.S * Jamie_Mitco_SPB_13.04338.04338.2 1.9706 0.2012 99.8 1241.0721 1241.4331 4960.4 1 4.188 77.8 1 R.TKSLEHELKR.S * Jamie_Mitco_SPB_9.02974.02974.1 1.2202 0.0188 86.2 855.46 855.96643 5384.1 163 2.9 58.3 1 K.SLEHELK.R * Jamie_Mitco_SPB_22.07900.07900.2 1.9359 0.1274 98.2 1322.4922 1321.5107 6108.9 134 3.982 45.5 1 R.STELLETVSLTK.R * Jamie_Mitco_SPB_12.04120.04120.2 1.8524 0.0189 92.3 872.9522 872.0152 3256.5 25 3.127 91.7 1 K.QRLDLAR.Q * Jamie_Mitco_SPB_47.16828.16828.2 4.4487 0.3776 100.0 2727.132 2727.1277 8458.3 1 6.546 39.6 1 K.LLLLNTSAIQETASPLSQDELISLR.K * Jamie_Mitco_SPB_51.18227.18227.2 2.5724 0.2736 100.0 2805.7522 2807.1277 9028.1 3 5.274 29.2 1 K.LLLLNTSAIQETAS*PLSQDELISLR.K * Jamie_Mitco_SPB_40.14160.14160.2 2.4965 0.3293 100.0 2853.0923 2855.3018 7965.3 1 5.375 30.0 1 K.LLLLNTSAIQETASPLSQDELISLRK.I * Jamie_Mitco_SPB_42.15035.15035.2 1.9244 0.1745 98.9 2933.632 2935.3018 5556.2 5 4.107 28.0 1 K.LLLLNTSAIQETAS*PLSQDELISLRK.I * Jamie_Mitco_SPB_23.07983.07983.2 3.4182 0.2765 100.0 2246.632 2246.4363 6606.6 1 5.566 55.3 1 K.ILESSNIVNENDSQAIITER.L * Jamie_Mitco_SPB_24.08395.08395.2 2.3662 0.1421 99.8 2325.0923 2326.4363 8578.8 4 4.53 39.5 1 K.ILESSNIVNENDS*QAIITER.L * Jamie_Mitco_SPB_28.09965.09965.2 4.8326 0.4314 100.0 2327.392 2326.4363 10131.5 1 7.387 47.4 1 K.ILES*SNIVNENDSQAIITER.L * Jamie_Mitco_SPB_20.06855.06855.2 3.3391 0.3009 100.0 1548.2322 1549.721 8157.0 1 5.577 83.3 1 R.LVEFSNVNELQEK.N * Jamie_Mitco_SPB_19.06799.06799.1 3.1819 0.3495 100.0 1549.38 1549.721 5970.6 1 7.471 66.7 1 R.LVEFSNVNELQEK.N * Jamie_Mitco_SPB_35.12538.12538.2 3.9569 0.3472 100.0 2661.0522 2661.9812 7436.6 1 6.293 45.2 1 R.LVEFSNVNELQEKNVELLNCIR.I * Jamie_Mitco_SPB_19.06759.06759.1 1.7382 0.0394 96.3 1130.39 1131.2836 2946.4 375 4.673 50.0 1 K.NVELLNCIR.I * Jamie_Mitco_SPB_14.05022.05022.2 2.3179 0.1709 99.8 1763.0721 1764.9731 5217.6 49 4.436 46.4 1 R.ILADKLENYEGKQDK.T * Jamie_Mitco_SPB_27.09411.09411.4 4.8867 0.258 100.0 3376.1765 3376.832 7875.7 1 4.878 26.8 1 R.ILADKLENYEGKQDKTLQKVENQTIKEAK.D * Jamie_Mitco_SPB_58.20697.20697.4 4.5343 0.187 100.0 4703.7363 4701.3286 9640.8 1 4.441 20.0 1 R.ILADKLENYEGKQDKTLQKVENQTIKEAKDAIIELENINAK.M * Jamie_Mitco_SPB_11.03835.03835.3 3.1731 0.1868 80.0 1537.9443 1538.6543 5963.7 28 5.024 37.5 1 L.ADKLENYEGKQDK.T * Jamie_Mitco_SPB_13.04527.04527.2 3.728 0.3289 100.0 1630.4722 1630.8821 7561.9 1 6.745 69.2 1 K.TLQKVENQTIKEAK.D * Jamie_Mitco_SPB_51.18275.18275.4 5.2957 0.2485 100.0 2955.5764 2955.3787 9492.1 3 4.76 29.3 2 K.TLQKVENQTIKEAKDAIIELENINAK.M * Jamie_Mitco_SPB_49.17542.17542.3 5.2427 0.3143 100.0 2958.0842 2955.3787 9175.3 1 6.301 34.0 1 K.TLQKVENQTIKEAKDAIIELENINAK.M * Jamie_Mitco_SPB_1.00353.00353.2 2.6351 0.234 100.0 1159.5122 1160.3127 6630.0 3 5.388 72.2 1 K.VENQTIKEAK.D * Jamie_Mitco_SPB_37.13223.13223.4 3.7862 0.0865 86.4 2486.8564 2484.8096 6044.1 45 3.233 27.8 1 K.VENQTIKEAKDAIIELENINAK.M * Jamie_Mitco_SPB_24.08524.08524.1 3.4057 0.0378 100.0 1344.35 1343.5199 5943.3 1 3.763 68.2 2 K.DAIIELENINAK.M * Jamie_Mitco_SPB_28.10059.10059.2 4.3366 0.4687 100.0 1860.7722 1861.1205 9804.2 1 8.1 66.7 1 K.DAIIELENINAKMETR.I * Jamie_Mitco_SPB_23.07944.07944.3 3.0453 0.1193 93.8 2832.0842 2832.0686 7905.4 107 3.635 22.0 1 R.DSYKLLASTEENKANTNSVTSMEAAR.E * Jamie_Mitco_SPB_18.06292.06292.2 4.6978 0.5021 100.0 2832.112 2832.0686 5580.4 1 8.662 48.0 1 R.DSYKLLASTEENKANTNSVTSMEAAR.E * Jamie_Mitco_SPB_12.04077.04077.2 2.6781 0.2452 97.1 1537.8322 1538.6727 4411.9 1 4.673 57.7 1 A.NTNSVTSMEAAREK.K * Jamie_Mitco_SPB_30.10600.10600.3 3.4644 0.2055 100.0 2742.1143 2743.133 7275.4 2 4.255 30.4 1 K.KIRELEAELSSTKVENSAIIQNLR.K * Jamie_Mitco_SPB_29.10229.10229.4 4.9279 0.2934 100.0 2871.8564 2871.3071 6722.5 1 5.637 29.9 1 K.KIRELEAELSSTKVENSAIIQNLRK.E * Jamie_Mitco_SPB_15.05099.05099.2 3.0701 0.1427 100.0 1378.0521 1376.5498 7400.0 1 4.367 68.2 1 K.IRELEAELSSTK.V * Jamie_Mitco_SPB_27.09624.09624.3 4.1173 0.26 100.0 2613.6543 2614.959 8912.5 5 5.032 26.1 1 K.IRELEAELSSTKVENSAIIQNLR.K * Jamie_Mitco_SPB_26.09267.09267.4 4.4029 0.2844 100.0 2744.8965 2743.133 6938.3 1 5.367 27.5 1 K.IRELEAELSSTKVENSAIIQNLRK.E * Jamie_Mitco_SPB_14.04719.04719.1 2.9706 0.3057 100.0 1107.42 1107.2029 3062.8 1 6.316 77.8 1 R.ELEAELSSTK.V * Jamie_Mitco_SPB_26.09213.09213.3 3.1995 0.2094 100.0 2344.2244 2345.612 6252.4 4 5.629 31.2 1 R.ELEAELSSTKVENSAIIQNLR.K * Jamie_Mitco_SPB_23.08160.08160.3 5.3016 0.4573 100.0 2471.6943 2473.7861 6895.7 1 6.876 44.0 1 R.ELEAELSSTKVENSAIIQNLRK.E * Jamie_Mitco_SPB_23.08226.08226.2 1.7885 0.0104 85.4 2472.912 2473.7861 9037.1 307 3.181 26.2 1 R.ELEAELSSTKVENSAIIQNLRK.E * Jamie_Mitco_SPB_19.06525.06525.3 3.3759 0.0831 96.9 1501.9143 1500.6915 6860.2 17 3.776 47.7 1 K.KKTTLEDFENFK.G * Jamie_Mitco_SPB_20.06922.06922.2 2.8006 0.2001 100.0 1373.6522 1372.5175 3566.2 1 5.086 75.0 1 K.KTTLEDFENFK.G * Jamie_Mitco_SPB_23.08019.08019.3 4.1752 0.2795 100.0 1741.2843 1741.9817 5258.9 1 6.345 46.4 1 K.KTTLEDFENFKGLAK.E * Jamie_Mitco_SPB_22.07907.07907.2 2.6173 0.1726 100.0 1742.5922 1741.9817 7807.9 8 4.554 53.6 1 K.KTTLEDFENFKGLAK.E * Jamie_Mitco_SPB_25.08680.08680.1 2.2625 0.1805 100.0 1243.09 1244.3434 6340.8 28 5.724 61.1 1 K.TTLEDFENFK.G * Jamie_Mitco_SPB_26.09363.09363.2 3.2118 0.3635 100.0 1612.3522 1613.8076 9721.6 1 6.88 69.2 1 K.TTLEDFENFKGLAK.E * Jamie_Mitco_SPB_119.42556.42556.2 1.4629 0.0806 86.8 1948.6921 1951.0972 5008.8 258 3.006 33.3 1 K.T*TLEDFENFKGLAKEK.E * Jamie_Mitco_SPB_33.11819.11819.2 2.0389 0.081 96.3 1770.3322 1770.0509 7569.0 98 3.72 39.3 1 R.MLEEAIDHLKAELEK.Q * Jamie_Mitco_SPB_31.11020.11020.2 3.2303 0.4591 100.0 2024.9521 2026.3557 5634.8 1 7.119 56.2 1 R.MLEEAIDHLKAELEKQK.S * Jamie_Mitco_SPB_31.11001.11001.3 4.8011 0.3416 100.0 2028.4744 2026.3557 5513.3 1 6.906 45.3 1 R.MLEEAIDHLKAELEKQK.S * Jamie_Mitco_SPB_16.05465.05465.3 3.4994 0.3321 100.0 1949.0044 1950.2859 5079.6 2 5.7 35.9 1 K.LKKETASFIPTKESLTR.D * Jamie_Mitco_SPB_16.05739.05739.2 3.4294 0.2868 100.0 1581.3722 1580.7783 4001.2 1 5.208 69.2 1 K.ETASFIPTKESLTR.D * Jamie_Mitco_SPB_16.05757.05757.3 2.9861 0.3346 100.0 1582.3444 1580.7783 4078.8 3 5.237 40.4 1 K.ETASFIPTKESLTR.D * Jamie_Mitco_SPB_14.04998.04998.2 2.8127 0.3046 100.0 1574.0521 1573.632 3856.7 1 5.522 63.6 1 K.ESLTRDFEQCCK.E * Jamie_Mitco_SPB_1.00219.00219.2 3.5747 0.4065 100.0 1427.9922 1428.5419 5153.9 1 7.304 86.4 3 R.LKESEISHNENK.M * Jamie_Mitco_SPB_10.03297.03297.3 2.7325 0.1197 95.5 1428.7444 1428.5419 5128.1 3 4.287 47.7 1 R.LKESEISHNENK.M * Jamie_Mitco_SPB_14.04932.04932.3 3.2949 0.2247 100.0 2125.7043 2124.33 6639.8 7 4.642 35.3 1 R.LKESEISHNENKMDFSSK.E * Jamie_Mitco_SPB_13.04522.04522.3 3.062 0.2305 100.0 1884.4744 1882.9966 4989.9 133 4.967 31.7 1 K.ESEISHNENKMDFSSK.E * Jamie_Mitco_SPB_12.04311.04311.2 1.9748 0.0728 97.4 1019.1322 1017.0837 4668.3 10 3.197 71.4 1 K.ELENNLER.L * Jamie_Mitco_SPB_27.09574.09574.2 2.8749 0.213 100.0 1648.3922 1646.8381 6284.2 1 4.857 65.4 1 R.SDLQSKIQEIESIR.S * Jamie_Mitco_SPB_16.05589.05589.2 3.7279 0.3796 100.0 1710.6522 1711.9453 4945.2 1 6.458 76.9 1 K.WAQNTIDDTEMKMK.S * Jamie_Mitco_SPB_53.18750.18750.4 3.4186 0.1596 94.3 3493.5764 3493.9922 8551.4 3 4.04 21.8 1 K.MKSLLTELSNKETTIEKLSSEIENLDKELR.K * Jamie_Mitco_SPB_22.07692.07692.2 3.2266 0.3112 100.0 1706.5122 1706.931 8141.3 1 5.942 60.7 2 K.SLLTELSNKETTIEK.L * Jamie_Mitco_SPB_52.18413.18413.3 7.0332 0.3198 100.0 3234.5942 3234.6257 6549.2 1 7.22 39.8 2 K.SLLTELSNKETTIEKLSSEIENLDKELR.K * Jamie_Mitco_SPB_52.18621.18621.4 6.8204 0.3671 100.0 3234.7366 3234.6257 8329.4 1 6.375 38.9 1 K.SLLTELSNKETTIEKLSSEIENLDKELR.K * Jamie_Mitco_SPB_50.17754.17754.4 5.3942 0.3281 100.0 3464.8164 3463.9048 8429.7 1 5.306 29.3 1 K.SLLTELSNKETTIEKLSSEIENLDKELRKT.K * Jamie_Mitco_SPB_42.14769.14769.4 6.8598 0.3436 100.0 3034.3765 3034.388 11522.8 1 8.251 40.7 1 L.LTELSNKETTIEKLSSEIENLDKELR.K * Jamie_Mitco_SPB_15.05117.05117.1 2.0871 0.0193 96.2 1148.54 1148.2554 4202.8 4 3.407 66.7 2 K.LSSEIENLDK.E * Jamie_Mitco_SPB_19.06537.06537.3 3.5554 0.2284 100.0 1674.8944 1674.8918 4110.8 1 4.692 51.9 1 K.LSSEIENLDKELRK.T * Jamie_Mitco_SPB_19.06549.06549.2 4.244 0.3027 100.0 1675.5721 1674.8918 5515.7 1 5.694 73.1 1 K.LSSEIENLDKELRK.T * Jamie_Mitco_SPB_23.08200.08200.2 2.3648 0.0508 96.8 2503.892 2502.7864 7164.5 3 3.463 35.0 1 K.FQYKFLDQNSDASTLEPTLRK.E * Jamie_Mitco_SPB_24.08307.08307.3 3.2594 0.049 92.9 2504.3342 2502.7864 8114.2 1 4.603 38.8 1 K.FQYKFLDQNSDASTLEPTLRK.E * Jamie_Mitco_SPB_23.07966.07966.2 2.4385 0.1508 99.8 1809.0721 1807.9548 8444.9 4 4.292 46.7 1 K.FLDQNSDASTLEPTLR.K * Jamie_Mitco_SPB_18.06375.06375.3 2.7275 0.0303 85.4 1935.0543 1936.1289 4965.3 2 2.987 43.8 1 K.FLDQNSDASTLEPTLRK.E * Jamie_Mitco_SPB_18.06354.06354.2 2.916 0.313 100.0 1935.8922 1936.1289 7994.2 1 5.335 53.1 1 K.FLDQNSDASTLEPTLRK.E * Jamie_Mitco_SPB_44.15503.15503.3 3.8097 0.2882 100.0 3144.3843 3145.537 7646.1 1 5.034 26.9 1 K.FLDQNSDASTLEPTLRKELEQIQVQLK.D * Jamie_Mitco_SPB_70.25181.25181.4 6.4376 0.4245 100.0 6377.2964 6376.07 10095.5 1 6.651 16.1 2 K.FLDQNSDASTLEPTLRKELEQIQVQLKDANSQIQAYEEIISSNENALIELKNELAK.T * Jamie_Mitco_SPB_58.20673.20673.4 8.1649 0.5108 100.0 4586.0967 4587.138 11257.8 1 8.165 27.8 1 R.KELEQIQVQLKDANSQIQAYEEIISSNENALIELKNELAK.T * Jamie_Mitco_SPB_61.21909.21909.3 6.261 0.5358 100.0 4457.9043 4458.964 9671.6 1 8.126 25.0 2 K.ELEQIQVQLKDANSQIQAYEEIISSNENALIELKNELAK.T * Jamie_Mitco_SPB_62.22023.22023.4 7.8368 0.4433 100.0 4460.9766 4458.964 9996.9 1 7.276 21.5 1 K.ELEQIQVQLKDANSQIQAYEEIISSNENALIELKNELAK.T * 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Jamie_Mitco_SPB_14.05021.05021.2 2.4674 0.0578 98.3 1711.5521 1709.9371 5757.8 1 4.143 53.8 4 K.TKENYDAKIELEKK.E * Jamie_Mitco_SPB_20.06939.06939.2 2.2486 0.1797 99.8 1966.7322 1967.2267 6214.9 1 4.107 46.7 1 K.TKENYDAKIELEKKEK.W * Jamie_Mitco_SPB_121.43332.43332.4 2.8269 0.1716 94.5 1967.8965 1967.2267 6704.0 188 4.082 31.1 1 K.TKENYDAKIELEKKEK.W * Jamie_Mitco_SPB_14.04689.04689.3 1.795 0.1309 88.1 1418.9944 1419.537 3694.7 33 4.103 40.0 1 K.EKWAREEDLSR.L * Jamie_Mitco_SPB_25.08860.08860.3 4.0557 0.3637 100.0 2509.2244 2511.8435 6311.9 1 5.887 38.8 2 K.LKEGALHFVQQSEKLRNEVER.I * Jamie_Mitco_SPB_1.00047.00047.2 2.3489 0.147 100.0 915.8122 916.02496 5982.2 4 4.02 83.3 3 K.LRNEVER.I * Jamie_Mitco_SPB_24.08488.08488.3 3.1425 0.184 99.5 2784.2644 2787.219 8247.2 293 4.217 23.9 1 K.KKEMSQYQSTMKENKDLSELVIR.L * Jamie_Mitco_SPB_12.04295.04295.2 1.8889 0.062 94.6 1233.5322 1233.3967 5050.0 34 3.242 66.7 1 K.EMSQYQSTMK.E * Jamie_Mitco_SPB_12.04125.04125.2 3.2919 0.3181 100.0 1604.4122 1604.7902 3603.4 1 5.673 75.0 1 K.EMSQYQSTMKENK.D * Jamie_Mitco_SPB_24.08512.08512.1 2.1208 0.0667 100.0 944.43 945.1039 3737.5 5 4.67 71.4 1 K.DLSELVIR.L * Jamie_Mitco_SPB_50.17899.17899.2 2.0536 0.2532 100.0 2516.612 2518.79 8720.2 113 4.099 23.8 1 K.DLSELVIRLEKDAADCQAELTK.T * Jamie_Mitco_SPB_49.17610.17610.3 4.5356 0.3781 100.0 2520.8643 2518.79 11135.4 1 6.651 38.1 1 K.DLSELVIRLEKDAADCQAELTK.T * Jamie_Mitco_SPB_13.04350.04350.1 2.1712 0.3181 100.0 1591.32 1592.7095 5763.2 1 5.959 53.8 1 R.LEKDAADCQAELTK.T * Jamie_Mitco_SPB_12.04324.04324.3 3.0099 0.2459 100.0 1592.1543 1592.7095 5132.3 1 5.477 46.2 1 R.LEKDAADCQAELTK.T * Jamie_Mitco_SPB_1.00022.00022.2 2.9262 0.2671 100.0 1592.6721 1592.7095 8672.3 1 5.317 69.2 2 R.LEKDAADCQAELTK.T * Jamie_Mitco_SPB_10.03252.03252.2 1.6935 0.0419 88.7 1223.1721 1222.2604 4231.8 92 3.31 60.0 1 K.DAADCQAELTK.T * Jamie_Mitco_SPB_17.05865.05865.3 2.8294 0.326 100.0 1762.1943 1762.917 4631.3 1 5.421 55.4 1 K.SSLYSAQDLLDKHER.K * Jamie_Mitco_SPB_18.06449.06449.2 2.588 0.1823 100.0 1763.2722 1762.917 6901.8 4 4.535 46.4 1 K.SSLYSAQDLLDKHER.K * Jamie_Mitco_SPB_37.13216.13216.4 3.967 0.2851 100.0 3001.6565 2999.322 6986.1 2 4.806 26.8 1 R.KWMEEKADYERELISNIEQTESLR.V * Jamie_Mitco_SPB_38.13375.13375.2 1.6426 0.1317 94.8 2870.392 2871.148 9765.0 2 3.9 27.3 1 K.WMEEKADYERELISNIEQTESLR.V * Jamie_Mitco_SPB_38.13470.13470.3 4.374 0.3887 100.0 2872.0144 2871.148 7275.9 1 6.21 37.5 1 K.WMEEKADYERELISNIEQTESLR.V * Jamie_Mitco_SPB_42.15106.15106.4 3.7591 0.1009 87.9 3882.7766 3883.319 8117.1 268 3.8 17.7 1 K.WMEEKADYERELISNIEQTESLRVENSVLIEK.V * Jamie_Mitco_SPB_22.07620.07620.2 3.5567 0.4175 100.0 1532.3722 1532.6909 5542.9 1 7.031 75.0 1 R.ELISNIEQTESLR.V * Jamie_Mitco_SPB_6.01809.01809.2 3.6048 0.3393 100.0 1612.8322 1613.6812 6392.1 4 6.257 50.0 3 K.VDDTAANNGDKDHLK.L * Jamie_Mitco_SPB_30.10791.10791.4 4.4196 0.3265 100.0 2643.2966 2643.9163 7162.0 1 5.9 29.7 2 K.VDDTAANNGDKDHLKLVSLFSNLR.H * Jamie_Mitco_SPB_29.10147.10147.2 3.0137 0.3183 100.0 2643.872 2643.9163 6276.2 1 5.292 39.1 2 K.VDDTAANNGDKDHLKLVSLFSNLR.H * Jamie_Mitco_SPB_30.10539.10539.3 3.3776 0.1392 98.2 2646.0842 2643.9163 9069.4 1 3.989 29.3 1 K.VDDTAANNGDKDHLKLVSLFSNLR.H * Jamie_Mitco_SPB_28.09897.09897.4 3.8801 0.162 96.8 3067.8164 3066.3604 5479.5 2 4.046 25.0 1 K.VDDTAANNGDKDHLKLVSLFSNLRHER.N * Jamie_Mitco_SPB_26.09047.09047.2 2.324 0.323 100.0 1049.3922 1049.2584 4354.9 1 6.026 93.8 1 K.LVSLFSNLR.H * Jamie_Mitco_SPB_38.13605.13605.3 5.3763 0.4264 100.0 2845.6143 2848.1426 9086.0 1 7.968 33.7 1 R.TQTLSEKEYQCSAVIIDEFKDITK.E * Jamie_Mitco_SPB_54.19149.19149.4 6.1976 0.3318 100.0 4786.2563 4784.3813 8834.6 1 4.861 21.2 1 R.TQTLSEKEYQCSAVIIDEFKDITKEVTQVNILKENNAILQK.S * Jamie_Mitco_SPB_19.06736.06736.2 4.4779 0.2961 100.0 1955.3522 1955.2621 7645.9 1 5.937 62.5 1 K.EVTQVNILKENNAILQK.S * Jamie_Mitco_SPB_9.02982.02982.1 2.055 0.1049 100.0 929.46 930.0489 5485.9 1 3.761 78.6 1 K.ENNAILQK.S * Jamie_Mitco_SPB_4.01089.01089.1 2.001 0.137 100.0 918.19 919.066 5426.3 14 4.229 64.3 1 K.SLKNVTEK.N * Jamie_Mitco_SPB_4.01143.01143.2 1.8908 0.0304 93.0 919.03217 919.066 5476.1 14 3.623 78.6 2 K.SLKNVTEK.N * Jamie_Mitco_SPB_10.03525.03525.2 1.9363 0.0382 92.2 1396.2322 1394.5706 6204.6 7 3.113 60.0 1 K.NVTEKNREIYK.Q * Jamie_Mitco_SPB_14.04983.04983.4 3.1742 0.1039 86.9 2193.0964 2192.3965 6144.8 18 4.052 32.3 1 K.NREIYKQLNDRQEEISR.L * Jamie_Mitco_SPB_22.07841.07841.3 3.7166 0.1615 99.5 2815.4644 2814.106 7594.6 1 4.064 31.8 1 K.EQVSINSNKILVYESEMEQCKQR.Y * Jamie_Mitco_SPB_18.06383.06383.2 2.2388 0.0309 96.2 1530.1122 1529.7114 7217.9 1 3.548 77.3 1 K.ILVYESEMEQCK.Q * Jamie_Mitco_SPB_15.05359.05359.2 3.4829 0.3642 100.0 1813.2722 1814.0297 4456.3 1 6.079 69.2 2 K.ILVYESEMEQCKQR.Y * Jamie_Mitco_SPB_8.02795.02795.1 1.9147 0.1557 100.0 1138.2 1138.2224 5587.6 4 4.585 62.5 1 R.YQDLSQQQK.D * Jamie_Mitco_SPB_8.02679.02679.2 2.6191 0.141 100.0 1140.3522 1138.2224 5215.9 2 4.107 87.5 1 R.YQDLSQQQK.D * Jamie_Mitco_SPB_7.02368.02368.2 3.9693 0.3797 100.0 1579.6921 1580.6946 7205.7 1 7.802 83.3 1 R.YQDLSQQQKDAQK.K * Jamie_Mitco_SPB_12.04185.04185.3 3.0747 0.1189 95.8 2196.4143 2194.4062 4904.8 1 4.27 38.2 1 R.YQDLSQQQKDAQKKDIEK.L * Jamie_Mitco_SPB_28.09771.09771.3 5.0178 0.4187 100.0 3393.2644 3393.776 8617.7 1 6.174 30.4 1 R.YQDLSQQQKDAQKKDIEKLTNEISDLKGK.L * Jamie_Mitco_SPB_25.08889.08889.3 3.4119 0.0557 93.1 2273.8743 2274.5767 6645.6 1 3.205 39.5 1 K.DAQKKDIEKLTNEISDLKGK.L * Jamie_Mitco_SPB_24.08505.08505.4 4.0551 0.277 100.0 2274.7766 2274.5767 6124.6 3 5.2 37.7 1 K.DAQKKDIEKLTNEISDLKGK.L * Jamie_Mitco_SPB_37.13132.13132.3 2.9739 0.1411 95.1 3580.9143 3578.915 8668.8 2 3.343 21.0 1 K.DIEKLTNEISDLKGKLSSAENANADLENKFNR.L * Jamie_Mitco_SPB_37.13095.13095.4 5.3478 0.3433 100.0 3582.7366 3578.915 8075.3 1 6.323 25.3 1 K.DIEKLTNEISDLKGKLSSAENANADLENKFNR.L * Jamie_Mitco_SPB_28.09762.09762.4 3.4232 0.0873 84.8 3094.7766 3093.3774 7286.7 23 4.205 23.5 1 K.LTNEISDLKGKLSSAENANADLENKFNR.L * Jamie_Mitco_SPB_40.14135.14135.4 3.2923 0.2705 90.5 2767.4966 2765.0093 7906.6 24 4.3 23.6 1 N.EISDLKGKLSSAENANADLENKFNR.L * Jamie_Mitco_SPB_15.05158.05158.2 4.6712 0.3787 100.0 2078.8123 2079.234 5528.7 1 7.004 61.1 1 K.GKLSSAENANADLENKFNR.L * Jamie_Mitco_SPB_13.04366.04366.1 2.4241 0.3216 100.0 1475.3 1476.54 6282.4 1 5.592 57.7 1 K.LSSAENANADLENK.F * Jamie_Mitco_SPB_15.05308.05308.2 4.3297 0.5547 100.0 1892.2922 1894.0079 6359.6 1 9.748 59.4 3 K.LSSAENANADLENKFNR.L * Jamie_Mitco_SPB_18.06325.06325.2 2.9549 0.1834 100.0 1601.1721 1600.8125 6942.5 8 4.52 50.0 1 K.KQQAALTNELNELK.A * Jamie_Mitco_SPB_47.16773.16773.4 7.7045 0.4893 100.0 3482.5764 3481.931 8062.7 1 7.923 33.9 1 K.KQQAALTNELNELKAIKDKLEQDLHFENAK.V * Jamie_Mitco_SPB_48.16943.16943.3 4.214 0.1959 100.0 3356.3044 3353.7568 5218.9 1 4.628 24.1 1 K.QQAALTNELNELKAIKDKLEQDLHFENAK.V * Jamie_Mitco_SPB_42.15033.15033.4 3.3227 0.2868 93.8 2738.0166 2741.0732 5957.6 7 5.197 27.3 1 T.NELNELKAIKDKLEQDLHFENAK.V * Jamie_Mitco_SPB_20.06966.06966.2 4.3302 0.4353 100.0 1898.9122 1900.1417 8187.3 1 7.771 66.7 2 K.AIKDKLEQDLHFENAK.V * Jamie_Mitco_SPB_19.06819.06819.3 3.8678 0.2455 100.0 1899.0543 1900.1417 8509.8 1 5.407 48.3 2 K.AIKDKLEQDLHFENAK.V * Jamie_Mitco_SPB_19.06838.06838.4 3.8722 0.1713 98.8 1903.5765 1900.1417 3804.7 1 4.223 40.0 1 K.AIKDKLEQDLHFENAK.V * Jamie_Mitco_SPB_15.05289.05289.3 3.0607 0.101 95.8 1586.9944 1587.7294 5381.0 46 4.057 45.8 1 K.DKLEQDLHFENAK.V * Jamie_Mitco_SPB_16.05573.05573.2 3.4012 0.1397 100.0 1588.9122 1587.7294 6697.9 1 4.146 75.0 4 K.DKLEQDLHFENAK.V * Jamie_Mitco_SPB_14.04819.04819.1 2.4108 0.303 100.0 1344.02 1344.4668 7728.9 2 5.981 55.0 1 K.LEQDLHFENAK.V * Jamie_Mitco_SPB_14.05031.05031.2 2.7396 0.2484 100.0 1044.5922 1045.2645 3104.9 1 5.381 87.5 1 K.VIDLDTKLK.A * Jamie_Mitco_SPB_15.05267.05267.3 3.0619 0.2563 100.0 2555.1543 2557.7393 6976.4 291 4.444 23.8 2 K.LKAHELQSEDVSRDHEKDTYR.T * Jamie_Mitco_SPB_14.04887.04887.4 3.6499 0.167 96.8 2556.9766 2557.7393 6137.8 1 4.559 34.2 2 K.LKAHELQSEDVSRDHEKDTYR.T * Jamie_Mitco_SPB_14.04870.04870.2 3.0769 0.4144 100.0 2557.2522 2557.7393 7493.4 1 6.844 40.0 1 K.LKAHELQSEDVSRDHEKDTYR.T * Jamie_Mitco_SPB_38.13354.13354.4 4.8431 0.3573 100.0 3859.1365 3860.2878 11265.7 101 5.249 17.2 1 K.LKAHELQSEDVSRDHEKDTYRTLMEEIESLKK.E * Jamie_Mitco_SPB_37.13183.13183.3 3.5938 0.1559 99.5 3861.1443 3860.2878 10748.0 8 4.691 21.0 1 K.LKAHELQSEDVSRDHEKDTYRTLMEEIESLKK.E * Jamie_Mitco_SPB_5.01507.01507.2 3.3986 0.3433 100.0 1271.4521 1271.3292 6983.1 1 7.459 85.0 5 K.AHELQSEDVSR.D * Jamie_Mitco_SPB_13.04578.04578.2 2.5026 0.0757 98.7 2317.4521 2316.4055 6642.9 11 3.558 36.1 2 K.AHELQSEDVSRDHEKDTYR.T * Jamie_Mitco_SPB_13.04330.04330.3 2.6716 0.1434 94.8 2317.9143 2316.4055 6803.4 1 4.304 37.5 1 K.AHELQSEDVSRDHEKDTYR.T * Jamie_Mitco_SPB_26.09046.09046.2 2.6023 0.1807 100.0 1322.5122 1321.572 6376.5 1 4.03 80.0 3 R.TLMEEIESLKK.E * Jamie_Mitco_SPB_51.18129.18129.3 2.7391 0.2519 100.0 2079.5344 2080.4878 2901.4 1 4.742 40.6 1 R.TLMEEIESLKKELQIFK.T * Jamie_Mitco_SPB_51.18124.18124.2 4.5182 0.3754 100.0 2081.372 2080.4878 11151.5 1 6.319 62.5 1 R.TLMEEIESLKKELQIFK.T * Jamie_Mitco_SPB_21.07233.07233.2 4.4409 0.3767 100.0 1915.7322 1916.0947 7684.4 1 7.575 68.8 2 K.ELQIFKTANSSSDAFEK.L * Jamie_Mitco_SPB_24.08615.08615.2 1.774 0.1768 98.6 2155.9722 2157.4282 7063.5 3 3.696 36.1 1 K.ELQIFKTANSSSDAFEKLK.V * Jamie_Mitco_SPB_13.04635.04635.2 3.5133 0.417 100.0 1397.8322 1398.5126 7256.8 1 7.23 75.0 1 K.TANSSSDAFEKLK.V * Jamie_Mitco_SPB_18.06341.06341.2 2.9656 0.1449 100.0 2017.1721 2017.3513 5322.8 2 4.702 50.0 1 K.LKVNMEKEKDRIIDER.T * Jamie_Mitco_SPB_19.06519.06519.4 3.3358 0.2132 100.0 2017.2965 2017.3513 3892.2 1 4.468 36.7 1 K.LKVNMEKEKDRIIDER.T * Jamie_Mitco_SPB_15.05291.05291.2 2.654 0.1875 100.0 1775.0521 1776.0178 4229.0 2 4.248 53.8 1 K.VNMEKEKDRIIDER.T * Jamie_Mitco_SPB_13.04421.04421.2 2.8061 0.1843 100.0 1409.2922 1408.5944 4884.5 1 4.593 75.0 1 R.IIDERTKEFEK.K * Jamie_Mitco_SPB_7.02332.02332.1 2.0418 0.046 97.1 974.4 974.1454 4087.6 11 3.706 64.3 1 K.KLQETLNK.S * Jamie_Mitco_SPB_7.02283.02283.2 3.0228 0.0596 100.0 975.65216 974.1454 5036.4 1 3.263 92.9 2 K.KLQETLNK.S * Jamie_Mitco_SPB_17.05952.05952.4 4.0796 0.1182 94.6 2872.6165 2872.1992 7298.0 1 4.332 28.5 1 K.KLQETLNKSTSSEAEYSKDIETLKK.E * Jamie_Mitco_SPB_17.05987.05987.3 3.8902 0.3544 100.0 2873.6643 2872.1992 10964.7 1 5.884 33.3 1 K.KLQETLNKSTSSEAEYSKDIETLKK.E * Jamie_Mitco_SPB_40.14338.14338.4 5.8109 0.4231 100.0 4464.8965 4464.9243 10501.0 1 6.762 19.9 1 K.KLQETLNKSTSSEAEYSKDIETLKKEWLKEYEDETLR.R * Jamie_Mitco_SPB_40.14055.14055.4 5.7338 0.1466 100.0 4624.3364 4624.9243 10074.8 1 5.826 23.6 1 K.KLQETLNKSTSSEAEYSKDIETLKKEWLKEY*EDET*LR.R * Jamie_Mitco_SPB_7.02235.02235.2 1.9851 0.011 94.2 844.9922 845.9713 3493.7 76 2.943 83.3 1 K.LQETLNK.S * Jamie_Mitco_SPB_13.04589.04589.2 1.515 0.0997 89.5 1995.5122 1996.049 4042.8 31 3.791 40.6 1 K.LQETLNKSTS*SEAEYSK.D * Jamie_Mitco_SPB_17.05771.05771.4 2.6841 0.1221 82.6 2742.8564 2744.0251 5039.7 17 3.277 25.4 1 K.LQETLNKSTSSEAEYSKDIETLKK.E * Jamie_Mitco_SPB_18.06155.06155.2 1.9368 0.0534 91.7 1867.8121 1868.9031 6490.4 227 3.025 40.0 1 K.STSS*EAEYSKDIETLK.K * Jamie_Mitco_SPB_15.05217.05217.2 2.5527 0.0906 98.9 1916.3922 1917.0771 3969.8 18 4.232 37.5 2 K.STSSEAEYSKDIETLKK.E * Jamie_Mitco_SPB_40.14385.14385.4 5.8379 0.4196 100.0 3508.8564 3509.8022 7623.9 1 7.49 29.8 1 K.STSSEAEYSKDIETLKKEWLKEYEDETLR.R * Jamie_Mitco_SPB_41.14475.14475.3 3.1603 0.1466 96.5 3511.1343 3509.8022 8558.1 5 3.825 24.1 1 K.STSSEAEYSKDIETLKKEWLKEYEDETLR.R * Jamie_Mitco_SPB_39.13938.13938.3 4.9835 0.399 100.0 3665.4844 3665.9897 8238.0 1 6.761 31.0 1 K.STSSEAEYSKDIETLKKEWLKEYEDETLRR.I * Jamie_Mitco_SPB_38.13552.13552.4 5.5141 0.3229 100.0 3668.0566 3665.9897 9335.3 1 5.845 27.0 2 K.STSSEAEYSKDIETLKKEWLKEYEDETLRR.I * Jamie_Mitco_SPB_22.07623.07623.2 2.3636 0.1575 99.8 1611.0521 1611.7484 6475.9 1 4.156 68.2 1 K.EWLKEYEDETLR.R * Jamie_Mitco_SPB_20.07173.07173.2 3.1214 0.3267 100.0 1767.0721 1767.9359 4419.9 1 6.641 79.2 1 K.EWLKEYEDETLRR.I * Jamie_Mitco_SPB_20.07179.07179.3 2.064 0.0974 86.5 1768.7344 1767.9359 5248.6 30 4.197 39.6 1 K.EWLKEYEDETLRR.I * Jamie_Mitco_SPB_11.03729.03729.2 2.4259 0.074 99.0 1231.0322 1230.4069 4441.6 1 3.177 77.8 1 R.RIKEAEENLK.K * Jamie_Mitco_SPB_11.03870.03870.3 2.4209 0.0423 88.3 1231.4644 1230.4069 3460.1 6 3.348 50.0 1 R.RIKEAEENLK.K * Jamie_Mitco_SPB_9.03184.03184.1 2.4497 0.111 100.0 1074.34 1074.2194 4688.9 5 4.159 68.8 1 R.IKEAEENLK.K * Jamie_Mitco_SPB_5.01653.01653.2 2.523 0.2089 100.0 1075.1921 1074.2194 5625.7 15 4.208 75.0 4 R.IKEAEENLK.K * Jamie_Mitco_SPB_12.04005.04005.2 3.5528 0.2922 100.0 1358.3322 1358.5809 6617.1 1 6.301 85.0 5 R.IKEAEENLKKR.I * Jamie_Mitco_SPB_12.04043.04043.3 2.7851 0.0387 91.7 1360.7043 1358.5809 3466.2 17 3.871 47.5 3 R.IKEAEENLKKR.I * Jamie_Mitco_SPB_13.04553.04553.2 2.8211 0.3042 100.0 1366.3922 1365.4857 5700.9 1 6.153 77.8 4 K.RKEELEEEFR.K * Jamie_Mitco_SPB_18.06141.06141.3 2.8736 0.1718 99.5 1367.7244 1365.4857 6262.7 10 4.001 38.9 5 K.RKEELEEEFR.K * Jamie_Mitco_SPB_15.05199.05199.2 3.5526 0.3344 100.0 1494.5521 1493.6598 4175.8 1 5.208 80.0 3 K.RKEELEEEFRK.K * Jamie_Mitco_SPB_16.05440.05440.3 2.8483 0.1037 95.8 1495.2843 1493.6598 6658.4 220 3.124 40.0 1 K.RKEELEEEFRK.K * Jamie_Mitco_SPB_14.04906.04906.3 3.4495 0.2046 100.0 1621.5543 1621.8339 6023.1 17 4.854 47.7 1 K.RKEELEEEFRKK.L * Jamie_Mitco_SPB_14.04897.04897.2 4.0362 0.1092 100.0 1623.0122 1621.8339 5918.1 1 4.641 77.3 3 K.RKEELEEEFRKK.L * Jamie_Mitco_SPB_14.04737.04737.3 2.7129 0.2148 100.0 1335.9844 1337.4723 7146.4 14 4.3 52.8 1 R.KEELEEEFRK.K * Jamie_Mitco_SPB_14.04756.04756.2 2.4986 0.2071 100.0 1465.2522 1465.6464 5345.0 2 4.126 65.0 1 R.KEELEEEFRKK.L * Jamie_Mitco_SPB_17.05901.05901.2 4.7095 0.3878 100.0 1678.5721 1678.9261 6839.2 1 7.803 85.7 1 K.KLKENAGSLTFLDNK.G * Jamie_Mitco_SPB_19.06533.06533.2 2.8187 0.0384 98.7 1552.0322 1550.7521 7445.9 41 3.444 53.8 1 K.LKENAGSLTFLDNK.G * Jamie_Mitco_SPB_28.09772.09772.3 4.7121 0.3495 100.0 3267.0842 3267.487 9421.4 1 6.081 26.7 1 K.LKENAGSLTFLDNKGSGEDAEEELWNSPSK.G * Jamie_Mitco_SPB_30.10715.10715.3 4.3578 0.4215 100.0 3346.8843 3347.487 4823.9 1 6.132 30.2 1 K.LKENAGSLTFLDNKGS*GEDAEEELWNSPSK.G * Jamie_Mitco_SPB_29.10099.10099.4 4.9995 0.309 100.0 4242.9766 4245.43 5355.7 2 5.79 18.0 1 K.LKENAGSLTFLDNKGSGEDAEEELWNS*PSKGNSERPSAV.A * Jamie_Mitco_SPB_30.10803.10803.4 5.1766 0.3405 100.0 4920.3765 4924.3057 7348.1 1 5.279 18.5 2 K.LKENAGSLTFLDNKGSGEDAEEELWNSPSKGNSERPSAVAGFINQK.N * Jamie_Mitco_SPB_31.11109.11109.4 4.1213 0.2337 100.0 5002.2563 5004.3057 4233.9 5 5.037 16.3 1 K.LKENAGSLTFLDNKGSGEDAEEELWNS*PSKGNSERPSAVAGFINQK.N * Jamie_Mitco_SPB_31.10990.10990.3 2.9674 0.286 100.0 5002.6743 5004.3057 3912.5 24 4.919 13.3 1 K.LKENAGSLTFLDNKGSGEDAEEELWNS*PSKGNSERPSAVAGFINQK.N * Jamie_Mitco_SPB_34.12112.12112.3 2.5462 0.0387 80.2 3105.5344 3106.1536 8031.4 17 3.34 23.1 1 K.ENAGSLT*FLDNKGSGEDAEEELWNSPSK.G * Jamie_Mitco_SPB_37.13157.13157.3 4.6848 0.3219 100.0 3186.1443 3186.1536 7607.3 7 6.179 24.1 1 K.ENAGSLTFLDNKGS*GEDAEEELWNS*PSK.G * Jamie_Mitco_SPB_18.06249.06249.1 2.4684 0.2736 100.0 1734.4 1735.7582 5579.4 2 5.952 43.3 1 K.GSGEDAEEELWNSPSK.G * Jamie_Mitco_SPB_17.06076.06076.2 4.7277 0.2474 100.0 1737.0721 1735.7582 8543.5 1 6.494 70.0 2 K.GSGEDAEEELWNSPSK.G * Jamie_Mitco_SPB_24.08467.08467.4 4.1264 0.2577 100.0 3395.8164 3392.5767 6608.7 1 4.696 25.3 1 K.GSGEDAEEELWNSPSKGNSERPSAVAGFINQK.N * Jamie_Mitco_SPB_16.05405.05405.2 2.8543 0.0551 98.9 1675.0721 1675.8418 6484.4 1 3.739 53.3 1 K.GNSERPSAVAGFINQK.N * Jamie_Mitco_SPB_10.03309.03309.2 1.8755 0.1698 99.6 1098.5122 1098.2877 4326.1 3 3.853 68.8 1 K.NLKPQEQLK.N * Jamie_Mitco_SPB_22.07791.07791.3 4.3457 0.4533 100.0 4407.2046 4407.813 7468.6 1 7.258 20.5 1 K.NLKPQEQLKNVKNDVSFNDSQSMVTNKENNIVDSSAAGNK.A * Jamie_Mitco_SPB_17.05949.05949.2 3.0044 0.2268 100.0 1686.7722 1686.7906 10313.5 1 5.627 60.7 2 K.NDVSFNDSQSMVTNK.E * Jamie_Mitco_SPB_11.03712.03712.2 3.0966 0.2409 100.0 1318.1122 1319.3708 6971.1 1 6.656 83.3 2 K.ENNIVDSSAAGNK.A * Jamie_Mitco_SPB_48.16953.16953.3 6.7364 0.559 100.0 4353.4146 4353.7944 7866.6 1 11.075 29.5 1 K.AIPTFSFGKPFFSSNTSSLQSFQNPFTASQSNINTNAPLR.T * Jamie_Mitco_SPB_48.17031.17031.4 5.5431 0.4428 100.0 4355.2563 4353.7944 7774.3 1 6.883 21.8 1 K.AIPTFSFGKPFFSSNTSSLQSFQNPFTASQSNINTNAPLR.T * Jamie_Mitco_SPB_18.06355.06355.2 3.6353 0.444 100.0 1973.8722 1974.1405 6914.9 1 6.955 52.9 1 F.QNPFTASQSNINTNAPLR.T * Jamie_Mitco_SPB_13.04476.04476.2 2.6876 0.2572 97.0 1485.8922 1487.6127 6711.3 75 4.528 42.3 1 F.TASQSNINTNAPLR.T * Jamie_Mitco_SPB_16.05655.05655.2 2.8382 0.2593 100.0 1211.1522 1212.4319 7621.9 1 5.894 75.0 1 R.TLNIQPEVAVK.A * Jamie_Mitco_SPB_25.08853.08853.2 4.2802 0.5178 100.0 2053.0122 2054.1777 7515.0 1 9.953 60.5 4 K.AAINFSNVTDLTNNSTDGAK.I * Jamie_Mitco_SPB_25.08956.08956.2 3.7425 0.4269 100.0 1982.0922 1983.099 8709.4 2 7.31 44.4 1 A.AINFSNVTDLTNNSTDGAK.I * Jamie_Mitco_SPB_24.08469.08469.3 4.2113 0.2831 100.0 1984.5543 1983.099 6026.4 1 5.661 43.1 1 A.AINFSNVTDLTNNSTDGAK.I * Jamie_Mitco_SPB_8.02803.02803.1 1.9425 0.2662 100.0 935.75 936.0501 4412.2 1 5.483 75.0 2 K.ITEIGSTSK.R * Jamie_Mitco_SPB_28.10045.10045.2 1.5253 0.1199 90.7 2565.9722 2567.5938 7288.7 69 3.592 22.7 1 K.ITEIGST*S*KRPIESGTSSDPDTK.K * Jamie_Mitco_SPB_5.01563.01563.3 3.8651 0.3786 100.0 1618.7644 1618.741 7629.4 1 6.787 48.2 1 K.RPIESGTSSDPDTKK.V * Jamie_Mitco_SPB_1.00180.00180.2 2.1546 0.1695 99.8 1619.1322 1618.741 4504.0 3 4.111 50.0 1 K.RPIESGTSSDPDTKK.V * Jamie_Mitco_SPB_9.03189.03189.3 2.639 0.1077 93.1 1846.4944 1846.0476 5481.5 48 3.657 34.4 1 K.RPIESGTSSDPDTKKVK.E YDR356W 153 194 74.9% 944 111782 7.1 U SPC110 SGDID:S000002764, Chr IV from 1186098-1188932, Verified ORF, "Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner" * Jamie_Mitco_SPB_34.12015.12015.1 1.8398 0.085 100.0 1363.39 1363.528 4024.5 14 3.46 55.0 1 K.NMEFT*PVGFIK.S * Jamie_Mitco_SPB_34.12039.12039.2 3.1154 0.3029 100.0 1363.7722 1363.528 6953.1 1 5.6 75.0 1 K.NMEFT*PVGFIK.S * Jamie_Mitco_SPB_9.03022.03022.2 3.0538 0.4092 100.0 1383.5122 1384.4429 5430.4 1 6.83 72.7 2 R.NTTQTQVVS*PTK.V * Jamie_Mitco_SPB_13.04482.04482.2 1.7613 0.2193 99.4 3123.4922 3124.3945 5429.2 1 4.084 28.6 1 R.NTTQTQVVSPTKVPNANNGDENEGPVKKR.Q * Jamie_Mitco_SPB_11.03664.03664.2 2.0402 0.0347 91.4 1555.4521 1554.6133 5404.0 42 3.388 42.9 2 K.VPNANNGDENEGPVK.K * Jamie_Mitco_SPB_9.02895.02895.2 2.6237 0.1361 99.8 1684.4321 1682.7875 6609.5 1 4.228 50.0 1 K.VPNANNGDENEGPVKK.R * Jamie_Mitco_SPB_47.16569.16569.3 3.616 0.345 100.0 2966.0645 2965.1536 8348.0 1 5.795 28.0 1 R.SIDDTIDSTRLFSEASQFDDSFPEIK.A * Jamie_Mitco_SPB_34.11940.11940.2 3.373 0.3443 100.0 2167.2722 2168.281 4156.7 1 5.922 50.0 1 T.RLFSEASQFDDS*FPEIKA.N * Jamie_Mitco_SPB_33.11793.11793.2 4.9111 0.5251 100.0 1860.3121 1861.0148 8382.6 1 9.455 80.0 1 R.LFSEASQFDDSFPEIK.A * Jamie_Mitco_SPB_38.13372.13372.2 3.6898 0.3845 100.0 1942.4922 1941.0148 7097.6 1 6.569 66.7 2 R.LFSEASQFDDS*FPEIK.A * Jamie_Mitco_SPB_47.16582.16582.2 2.0476 0.0697 95.6 2020.5521 2021.0148 4534.0 1 3.305 50.0 1 R.LFSEAS*QFDDS*FPEIK.A * Jamie_Mitco_SPB_10.03315.03315.2 1.9898 0.1324 99.4 931.3522 931.981 2794.6 60 4.441 64.3 3 K.ANIPPS*PR.S * Jamie_Mitco_SPB_31.10974.10974.3 4.19 0.331 100.0 2880.1743 2881.3635 8273.0 3 5.339 27.2 1 R.KRNLIDDLKKDVPMSQPLKEQEVR.E * Jamie_Mitco_SPB_31.10829.10829.2 2.6117 0.1319 99.8 2596.912 2597.002 10007.8 1 3.905 38.1 1 R.NLIDDLKKDVPMSQPLKEQEVR.E * Jamie_Mitco_SPB_31.10984.10984.3 6.1709 0.3687 100.0 2598.0244 2597.002 9090.1 1 6.983 40.5 1 R.NLIDDLKKDVPMSQPLKEQEVR.E * Jamie_Mitco_SPB_31.10860.10860.2 2.2326 0.2332 95.3 999.3122 999.15515 4053.3 1 4.9 78.6 1 Q.PLKEQEVR.E * Jamie_Mitco_SPB_17.05781.05781.3 2.2353 0.0871 90.8 1255.3143 1253.4874 6201.2 1 3.759 50.0 1 K.SLKHEIKELR.K * Jamie_Mitco_SPB_18.06456.06456.2 1.6484 0.1171 94.9 1381.9122 1381.6615 5888.7 22 3.332 50.0 1 K.SLKHEIKELRK.E * Jamie_Mitco_SPB_19.06693.06693.3 3.5071 0.1792 100.0 1382.2144 1381.6615 4665.9 2 4.129 50.0 1 K.SLKHEIKELRK.E * Jamie_Mitco_SPB_19.06739.06739.2 2.5397 0.3515 100.0 2797.6921 2798.934 5449.7 1 6.968 38.6 1 R.KEKNDTLNNYDTLEEETDDLKNR.L * Jamie_Mitco_SPB_19.06731.06731.3 5.0967 0.315 100.0 2800.6143 2798.934 8477.8 1 6.231 40.9 1 R.KEKNDTLNNYDTLEEETDDLKNR.L * Jamie_Mitco_SPB_23.07974.07974.2 3.3862 0.3763 100.0 2399.892 2400.4688 5868.3 1 6.123 39.5 2 K.EKNDTLNNYDTLEEETDDLK.N * Jamie_Mitco_SPB_23.08079.08079.3 2.8871 0.0437 88.8 2399.9644 2400.4688 9716.8 35 3.17 31.6 1 K.EKNDTLNNYDTLEEETDDLK.N * Jamie_Mitco_SPB_19.06815.06815.2 3.4784 0.4097 100.0 2669.7722 2670.76 3776.4 1 8.673 50.0 3 K.EKNDTLNNYDTLEEETDDLKNR.L * Jamie_Mitco_SPB_20.06869.06869.3 4.5621 0.3906 100.0 2672.5745 2670.76 5728.6 1 6.463 39.3 1 K.EKNDTLNNYDTLEEETDDLKNR.L * Jamie_Mitco_SPB_48.17049.17049.4 5.2546 0.2413 100.0 3911.2166 3910.1943 8927.4 12 5.182 19.8 2 K.EKNDTLNNYDTLEEETDDLKNRLQALEKELDAK.N * Jamie_Mitco_SPB_48.17218.17218.3 5.0886 0.3291 100.0 3911.5144 3910.1943 7346.8 1 5.831 23.4 1 K.EKNDTLNNYDTLEEETDDLKNRLQALEKELDAK.N * Jamie_Mitco_SPB_24.08610.08610.2 3.5058 0.4388 100.0 2141.4321 2143.1792 7671.3 1 7.9 67.6 8 K.NDTLNNYDTLEEETDDLK.N * Jamie_Mitco_SPB_22.07696.07696.2 4.578 0.424 100.0 2414.4722 2413.4705 8035.3 1 7.181 50.0 2 K.NDTLNNYDTLEEETDDLKNR.L * Jamie_Mitco_SPB_42.14799.14799.3 2.9641 0.098 92.9 3096.4443 3096.2886 9354.8 44 3.27 23.0 1 K.NDTLNNYDTLEEETDDLKNRLQALEK.E * Jamie_Mitco_SPB_50.17896.17896.4 3.5138 0.1346 93.2 3655.1765 3652.9048 6792.2 1 3.965 24.4 1 K.NDTLNNYDTLEEETDDLKNRLQALEKELDAK.N * Jamie_Mitco_SPB_14.05037.05037.3 2.6735 0.1845 99.5 1259.7244 1258.4575 4663.1 1 4.222 55.0 1 R.LQALEKELDAK.N * Jamie_Mitco_SPB_14.04708.04708.2 3.0238 0.1378 100.0 1501.7522 1500.7355 6498.9 2 4.249 66.7 1 R.LQALEKELDAKNK.I * Jamie_Mitco_SPB_16.05745.05745.4 3.4549 0.3637 100.0 2070.4165 2070.397 4144.0 1 5.907 37.3 1 R.LQALEKELDAKNKIVNSR.K * Jamie_Mitco_SPB_17.05884.05884.3 2.7013 0.2652 100.0 2073.1143 2070.397 6772.4 1 4.817 36.8 1 R.LQALEKELDAKNKIVNSR.K * Jamie_Mitco_SPB_1.00183.00183.2 3.423 0.4294 100.0 1444.9321 1445.495 5730.4 1 7.593 77.3 3 R.KVDDHSGCIEER.E * Jamie_Mitco_SPB_11.03747.03747.3 2.8507 0.1508 99.0 1445.1543 1445.495 6228.0 64 5.085 36.4 1 R.KVDDHSGCIEER.E * Jamie_Mitco_SPB_122.43805.43805.3 3.002 0.3323 100.0 2117.3342 2119.2366 7149.3 1 5.786 40.6 3 R.KVDDHSGCIEEREQMER.K * Jamie_Mitco_SPB_17.06054.06054.4 2.7738 0.1184 84.9 2123.1765 2119.2366 5733.2 2 3.311 37.5 1 R.KVDDHSGCIEEREQMER.K * Jamie_Mitco_SPB_11.03867.03867.2 2.5573 0.1329 100.0 859.6922 859.0134 3736.2 1 4.11 91.7 1 R.KLAELER.K * Jamie_Mitco_SPB_24.08620.08620.2 2.317 0.0674 97.5 2160.2722 2160.3862 9111.6 27 3.608 33.3 1 K.TVKDQVLELENNSDVQSLK.L * Jamie_Mitco_SPB_23.08025.08025.2 3.8946 0.3316 100.0 1831.3922 1831.9744 7250.6 1 6.537 60.0 2 K.DQVLELENNSDVQSLK.L * Jamie_Mitco_SPB_39.13816.13816.2 5.453 0.3636 100.0 2048.7722 2048.316 10469.5 1 6.991 68.8 2 R.SKEDELKNLMNELNELK.S * Jamie_Mitco_SPB_39.13895.13895.3 4.9679 0.2144 100.0 2050.8245 2048.316 6490.0 1 4.871 48.4 2 R.SKEDELKNLMNELNELK.S * Jamie_Mitco_SPB_42.15051.15051.4 5.9977 0.4088 100.0 3568.9365 3568.932 8802.1 1 6.768 25.9 2 R.SKEDELKNLMNELNELKSNAEEKDTQLEFK.K * Jamie_Mitco_SPB_43.15371.15371.3 3.0182 0.0405 88.1 3569.6343 3568.932 9906.4 3 3.433 23.3 1 R.SKEDELKNLMNELNELKSNAEEKDTQLEFK.K * Jamie_Mitco_SPB_39.13815.13815.3 4.5196 0.3474 100.0 4207.8843 4209.6724 7320.7 1 6.903 25.7 1 R.SKEDELKNLMNELNELKSNAEEKDTQLEFKKNELR.K * Jamie_Mitco_SPB_39.13896.13896.4 6.5927 0.47 100.0 4208.6963 4209.6724 8578.2 1 7.826 24.5 1 R.SKEDELKNLMNELNELKSNAEEKDTQLEFKKNELR.K * Jamie_Mitco_SPB_27.09621.09621.2 1.7319 0.0777 92.9 1218.1522 1218.4106 5308.2 16 3.722 61.1 1 K.NLMNELNELK.S * Jamie_Mitco_SPB_35.12358.12358.3 5.1818 0.3725 100.0 2739.1743 2739.0266 7070.9 1 6.812 39.8 1 K.NLMNELNELKSNAEEKDTQLEFK.K * Jamie_Mitco_SPB_13.04659.04659.2 3.0776 0.3037 100.0 1539.0721 1539.639 9980.2 1 5.97 70.8 1 K.SNAEEKDTQLEFK.K * Jamie_Mitco_SPB_14.04893.04893.2 2.1991 0.2088 100.0 882.09216 880.9732 4090.3 3 4.481 91.7 1 K.DTQLEFK.K * Jamie_Mitco_SPB_14.04842.04842.2 2.5493 0.1364 99.8 1520.8922 1521.7135 5281.7 17 4.392 54.5 1 K.DTQLEFKKNELR.K * Jamie_Mitco_SPB_25.08937.08937.3 4.5926 0.2965 100.0 2262.0244 2262.5806 8272.5 1 6.02 40.3 1 R.TNELNELKIKSDEMDLQLK.Q * Jamie_Mitco_SPB_16.05507.05507.1 1.9885 0.1008 100.0 1078.2 1079.2103 7391.2 5 4.864 62.5 1 K.SDEMDLQLK.Q * Jamie_Mitco_SPB_16.05482.05482.2 2.7236 0.2288 100.0 1080.4722 1079.2103 7668.8 3 5.47 87.5 1 K.SDEMDLQLK.Q * Jamie_Mitco_SPB_16.05763.05763.2 3.029 0.171 100.0 1489.8922 1488.6812 8338.1 1 4.11 77.3 1 K.RLKDELNELETK.F * Jamie_Mitco_SPB_16.05710.05710.3 3.5931 0.2141 100.0 1490.2444 1488.6812 6921.3 3 4.632 50.0 1 K.RLKDELNELETK.F * Jamie_Mitco_SPB_28.09931.09931.3 4.0564 0.3295 100.0 3224.7544 3225.4937 8833.6 30 5.503 22.2 1 K.RLKDELNELETKFSENGSQSSAKENELK.M * Jamie_Mitco_SPB_15.05129.05129.3 2.7043 0.1371 96.9 1330.9143 1332.4937 7672.9 2 4.001 57.5 1 R.LKDELNELETK.F * Jamie_Mitco_SPB_22.07717.07717.2 1.6377 0.0293 84.7 2455.152 2455.638 6090.9 98 3.276 28.6 1 R.LKDELNELETKFSENGSQSSAK.E * Jamie_Mitco_SPB_24.08289.08289.3 3.9689 0.1567 99.5 3069.4443 3069.3062 7828.3 1 4.577 32.7 1 R.LKDELNELETKFSENGSQSSAKENELK.M * Jamie_Mitco_SPB_23.08139.08139.4 4.5098 0.1764 100.0 3072.3765 3069.3062 3896.4 1 4.379 26.9 1 R.LKDELNELETKFSENGSQSSAKENELK.M * Jamie_Mitco_SPB_32.11268.11268.3 2.1388 0.0693 80.1 2370.0842 2374.3044 7473.5 104 3.111 28.9 1 K.DELNELET*KFSENGSQS*SAK.E * Jamie_Mitco_SPB_8.02836.02836.2 2.8362 0.2232 100.0 1755.6322 1755.8357 5437.2 1 5.629 53.3 2 K.FSENGSQSSAKENELK.M * Jamie_Mitco_SPB_22.07612.07612.1 2.5264 0.2891 100.0 1504.44 1504.6775 6000.0 1 5.934 50.0 1 K.NKIAELEEEISTK.N * Jamie_Mitco_SPB_21.07497.07497.2 3.627 0.2039 100.0 1505.2322 1504.6775 6251.2 1 6.226 83.3 3 K.NKIAELEEEISTK.N * Jamie_Mitco_SPB_27.09695.09695.2 2.2522 0.197 99.8 2257.892 2259.562 7846.5 7 4.246 31.6 1 K.NKIAELEEEISTKNSQLIAK.E * Jamie_Mitco_SPB_27.09509.09509.3 4.6678 0.3006 100.0 2262.1143 2259.562 4376.2 1 5.693 40.8 1 K.NKIAELEEEISTKNSQLIAK.E * Jamie_Mitco_SPB_27.09417.09417.4 4.2006 0.3282 100.0 2574.2566 2573.9036 7758.7 5 5.949 28.8 1 K.NKIAELEEEISTKNSQLIAKEGK.L * Jamie_Mitco_SPB_26.09359.09359.3 3.0354 0.2207 99.5 2575.3442 2573.9036 8624.3 94 3.711 22.7 1 K.NKIAELEEEISTKNSQLIAKEGK.L * Jamie_Mitco_SPB_16.05595.05595.2 4.2271 0.3725 100.0 1262.8922 1262.3997 8109.7 2 6.827 90.0 1 K.IAELEEEISTK.N * Jamie_Mitco_SPB_6.01974.01974.1 1.6876 0.02 95.4 773.23 773.9077 5601.6 5 2.797 66.7 1 K.NSQLIAK.E * Jamie_Mitco_SPB_5.01517.01517.2 2.6027 0.1991 100.0 1087.9722 1088.2493 4593.0 1 4.896 72.2 1 K.NSQLIAKEGK.L * Jamie_Mitco_SPB_5.01467.01467.1 1.8169 0.2198 100.0 1088.19 1088.2493 4158.6 1 5.093 66.7 1 K.NSQLIAKEGK.L * Jamie_Mitco_SPB_45.16147.16147.2 2.8382 0.2447 100.0 2360.2922 2359.7466 6752.2 1 4.481 37.5 1 K.EGKLASLMAQLTQLESKLNQR.D * Jamie_Mitco_SPB_36.12766.12766.2 3.8729 0.2885 100.0 1534.4122 1533.8236 9274.0 1 6.475 76.9 1 K.LASLMAQLTQLESK.L * Jamie_Mitco_SPB_43.15453.15453.2 4.0539 0.3066 100.0 2046.5721 2045.4052 9650.6 1 5.628 52.9 1 K.LASLMAQLTQLESKLNQR.D * Jamie_Mitco_SPB_13.04441.04441.1 2.4133 0.3002 100.0 1390.51 1391.4778 4049.4 4 6.189 54.5 1 R.DSQLGSREEELK.K * Jamie_Mitco_SPB_12.04215.04215.2 3.3634 0.1361 100.0 1520.8722 1519.6519 6486.7 2 4.022 70.8 1 R.DSQLGSREEELKK.T * Jamie_Mitco_SPB_21.07375.07375.3 2.6116 0.0502 84.7 2731.2244 2732.0232 6022.9 4 3.071 30.7 1 R.DSQLGSREEELKKTNDKLQKDIR.I * Jamie_Mitco_SPB_4.01283.01283.2 2.3239 0.0809 98.4 1603.7922 1603.8131 5463.1 5 3.525 58.3 1 R.EEELKKTNDKLQK.D * Jamie_Mitco_SPB_4.01422.01422.2 2.8566 0.1486 100.0 1231.9122 1231.3945 4944.3 3 3.884 77.8 1 K.TNDKLQKDIR.I * Jamie_Mitco_SPB_8.02751.02751.2 1.949 0.1433 98.7 1430.9922 1433.5614 4327.5 1 4.131 63.6 1 R.IAREETVSKDER.I * Jamie_Mitco_SPB_8.02782.02782.3 3.4948 0.1642 100.0 1433.3344 1433.5614 3684.9 1 5.913 50.0 1 R.IAREETVSKDER.I * Jamie_Mitco_SPB_21.07531.07531.2 3.4275 0.3041 100.0 1446.4922 1446.73 8981.9 1 6.757 77.3 1 K.VKQLENDLFVIK.K * Jamie_Mitco_SPB_12.04234.04234.2 3.8187 0.4344 100.0 1819.4521 1819.92 3748.3 1 8.498 60.0 1 K.THSESKTITDNELESK.D * Jamie_Mitco_SPB_15.05239.05239.2 1.8501 0.3083 100.0 2415.7122 2417.6758 7105.2 3 5.877 35.0 1 K.THSESKTITDNELESKDKLIK.I * Jamie_Mitco_SPB_15.05367.05367.2 4.0148 0.3745 100.0 1778.6721 1779.0435 4779.2 1 6.833 71.4 1 K.ILENDLKVAQEKYSK.M * Jamie_Mitco_SPB_19.06592.06592.2 2.7868 0.2928 100.0 2166.2122 2167.5256 6623.6 1 6.301 47.1 1 K.ILENDLKVAQEKYSKMEK.E * Jamie_Mitco_SPB_3.00953.00953.2 2.5108 0.1766 100.0 1064.3522 1063.2573 8019.6 3 4.388 78.6 2 K.MEKELKER.E * Jamie_Mitco_SPB_22.07893.07893.3 3.5849 0.1593 99.5 2547.7744 2546.7905 7995.3 1 3.946 37.5 1 R.EFNYKISESKLEDEKTTLNEK.I * Jamie_Mitco_SPB_26.09118.09118.4 4.758 0.3673 100.0 3134.4966 3134.465 7480.2 1 5.547 24.1 1 K.ISESKLEDEKTTLNEKISNLAAENSQLK.N * Jamie_Mitco_SPB_12.04066.04066.3 3.1663 0.2114 86.4 1450.7644 1448.6134 3326.7 27 4.475 43.2 1 S.KLEDEKTTLNEK.I * Jamie_Mitco_SPB_14.04827.04827.2 3.1789 0.2855 100.0 1288.2322 1288.4435 8095.7 1 5.16 81.8 1 K.ISNLAAENSQLK.N * Jamie_Mitco_SPB_17.05933.05933.3 2.8234 0.0416 85.1 3561.4143 3559.8896 8962.5 51 3.205 19.2 1 K.ISNLAAENSQLKNKIEDNSTATHHMKENYEK.Q * Jamie_Mitco_SPB_4.01443.01443.2 3.5737 0.3095 100.0 1626.2522 1626.7844 6375.1 1 6.408 69.2 2 K.NKIEDNSTATHHMK.E * Jamie_Mitco_SPB_13.04445.04445.2 3.6654 0.3919 100.0 2289.7122 2290.4692 7672.4 1 6.695 50.0 1 K.NKIEDNSTATHHMKENYEK.Q * Jamie_Mitco_SPB_36.12701.12701.3 3.0505 0.1598 96.8 3521.6943 3523.9194 6625.7 5 4.197 22.3 1 K.QLESLRKDIEEYKESAKDSEDKIEELKIR.I * Jamie_Mitco_SPB_36.12849.12849.4 4.3119 0.2732 100.0 3521.9766 3523.9194 7551.0 21 5.084 21.4 1 K.QLESLRKDIEEYKESAKDSEDKIEELKIR.I * Jamie_Mitco_SPB_10.03378.03378.1 1.7322 0.2073 100.0 1211.94 1212.2988 6417.9 32 4.964 50.0 1 K.DIEEYKESAK.D * Jamie_Mitco_SPB_10.03424.03424.2 2.1814 0.0090 94.1 1213.5721 1212.2988 5558.8 5 3.124 77.8 3 K.DIEEYKESAK.D * Jamie_Mitco_SPB_22.07714.07714.4 3.0641 0.1576 93.9 2665.2566 2668.9146 6955.7 16 4.71 26.2 2 K.DIEEYKESAKDSEDKIEELKIR.I * Jamie_Mitco_SPB_22.07763.07763.3 6.2231 0.4051 100.0 2669.5144 2668.9146 11165.9 1 7.853 38.1 1 K.DIEEYKESAKDSEDKIEELKIR.I * Jamie_Mitco_SPB_17.05817.05817.2 4.079 0.5297 100.0 2230.6921 2232.496 8999.4 1 8.858 63.9 1 R.SKDIKQKDEQISDLTQNLK.L * Jamie_Mitco_SPB_16.05763.05763.3 4.803 0.3503 100.0 2234.3342 2232.496 8338.1 1 6.343 44.4 1 R.SKDIKQKDEQISDLTQNLK.L * Jamie_Mitco_SPB_17.05765.05765.2 3.2928 0.2582 100.0 1661.6921 1660.8217 2638.5 1 5.248 73.1 1 K.QKDEQISDLTQNLK.L * Jamie_Mitco_SPB_21.07279.07279.1 2.109 0.1989 100.0 1404.82 1404.5168 5555.0 2 4.231 50.0 1 K.DEQISDLTQNLK.L * Jamie_Mitco_SPB_21.07252.07252.2 3.4936 0.2173 100.0 1404.9122 1404.5168 7792.4 1 5.372 81.8 1 K.DEQISDLTQNLK.L * Jamie_Mitco_SPB_37.13231.13231.2 2.2571 0.2002 100.0 2546.9321 2547.776 6354.4 1 4.467 38.1 1 K.DEQISDLTQNLKLQEDEISSLK.S * Jamie_Mitco_SPB_28.10023.10023.2 4.1035 0.3419 100.0 1747.2922 1746.9554 7754.5 1 7.425 75.0 1 K.LQEDEISSLKSIIDR.Y * Jamie_Mitco_SPB_24.08352.08352.2 2.5335 0.0678 98.7 1767.9722 1769.0537 5061.0 56 3.471 42.3 1 K.SIIDRYKKDFNQLK.S * Jamie_Mitco_SPB_50.17871.17871.4 5.7959 0.255 100.0 4102.2163 4101.609 9144.3 1 5.738 22.7 1 K.SIIDRYKKDFNQLKSEQSNIQHDLNLQILNLENK.L * Jamie_Mitco_SPB_38.13678.13678.4 4.1584 0.2632 100.0 3514.8564 3516.9358 9340.6 53 5.013 18.5 1 R.YKKDFNQLKSEQSNIQHDLNLQILNLENK.L * Jamie_Mitco_SPB_41.14553.14553.2 4.2555 0.3682 100.0 2498.872 2498.7551 8846.0 1 7.173 37.5 1 K.DFNQLKSEQSNIQHDLNLQIL.N * Jamie_Mitco_SPB_43.15363.15363.3 4.4508 0.3146 100.0 3098.9644 3097.4116 10633.4 1 5.506 29.0 1 K.DFNQLKSEQSNIQHDLNLQILNLENK.L * Jamie_Mitco_SPB_57.20356.20356.4 6.0764 0.4128 100.0 4153.6963 4154.577 9614.4 1 6.286 25.0 1 K.DFNQLKSEQSNIQHDLNLQILNLENKLIESEDELK.S * Jamie_Mitco_SPB_57.20460.20460.3 5.4175 0.3076 100.0 4155.2046 4154.577 10012.3 1 6.157 27.2 1 K.DFNQLKSEQSNIQHDLNLQILNLENKLIESEDELK.S * Jamie_Mitco_SPB_56.19896.19896.4 4.4483 0.1944 100.0 4968.177 4969.474 9751.5 1 3.843 17.5 1 K.DFNQLKSEQSNIQHDLNLQILNLENKLIESEDELKSLRDSQK.I * Jamie_Mitco_SPB_34.11938.11938.2 6.07 0.4571 100.0 2351.372 2351.5786 9925.0 1 7.578 57.9 1 K.SEQSNIQHDLNLQILNLENK.L * Jamie_Mitco_SPB_34.11991.11991.3 3.2522 0.1318 96.8 2351.6643 2351.5786 6806.9 2 4.101 34.2 1 K.SEQSNIQHDLNLQILNLENK.L * Jamie_Mitco_SPB_54.19146.19146.3 3.1024 0.1798 99.0 3409.1643 3408.7441 5600.6 27 3.334 24.1 1 K.SEQSNIQHDLNLQILNLENKLIESEDELK.S * Jamie_Mitco_SPB_57.20335.20335.4 4.6973 0.263 100.0 3766.2166 3765.1692 8779.9 25 4.758 19.9 1 K.SEQSNIQHDLNLQILNLENKLIESEDELKSLR.D * Jamie_Mitco_SPB_59.21130.21130.3 3.5038 0.3251 100.0 2260.0444 2257.5896 6527.8 1 5.691 36.1 1 Q.ILNLENKLIESEDELKSLR.D * Jamie_Mitco_SPB_35.12310.12310.2 3.2024 0.3457 100.0 1874.0721 1875.0831 6609.4 1 6.173 53.3 1 L.NLENKLIESEDELKSL.R * Jamie_Mitco_SPB_30.10452.10452.2 4.9119 0.394 100.0 2031.1322 2031.2706 7440.2 1 7.173 68.8 1 L.NLENKLIESEDELKSLR.D * Jamie_Mitco_SPB_29.10389.10389.3 5.3385 0.3514 100.0 2033.0343 2031.2706 8100.9 1 6.629 51.6 1 L.NLENKLIESEDELKSLR.D * Jamie_Mitco_SPB_37.13086.13086.4 6.2983 0.4116 100.0 3405.8965 3402.7832 9872.9 1 7.332 29.6 2 L.NLENKLIESEDELKSLRDSQKIEIENWK.R * Jamie_Mitco_SPB_16.05572.05572.2 3.5056 0.279 100.0 1433.6522 1432.614 7589.5 1 5.907 86.4 1 K.LIESEDELKSLR.D * Jamie_Mitco_SPB_15.05355.05355.3 2.8674 0.2358 100.0 1889.1244 1891.0856 3730.2 4 4.743 40.0 1 K.LIESEDELKSLRDSQK.I * Jamie_Mitco_SPB_27.09394.09394.3 5.53 0.315 100.0 2805.8943 2804.1265 8530.2 1 6.397 37.5 1 K.LIESEDELKSLRDSQKIEIENWK.R * Jamie_Mitco_SPB_16.05691.05691.3 2.6508 0.0368 90.0 1390.1344 1390.5358 5361.1 1 3.452 47.5 1 R.DSQKIEIENWK.R * Jamie_Mitco_SPB_17.05937.05937.2 2.5835 0.0547 98.9 1391.1122 1390.5358 5310.2 2 3.784 70.0 1 R.DSQKIEIENWK.R * Jamie_Mitco_SPB_17.05809.05809.3 3.4391 0.3018 100.0 2852.3643 2854.1033 8437.4 15 5.321 25.0 1 R.KYNNLSLENDRLLTEKESASDKER.E * Jamie_Mitco_SPB_25.08767.08767.4 3.7893 0.1959 98.8 3552.9365 3551.8926 6637.3 1 4.921 22.4 1 K.YNNLSLENDRLLTEKESASDKEREISILNR.K * Jamie_Mitco_SPB_16.05513.05513.2 3.4361 0.3938 100.0 2020.6522 2022.2806 5700.2 1 6.965 56.7 1 R.KLDEMDKEKWNLQESK.E * Jamie_Mitco_SPB_16.05409.05409.3 3.6471 0.2462 100.0 2024.7544 2022.2806 7257.3 1 4.787 40.0 1 R.KLDEMDKEKWNLQESK.E * Jamie_Mitco_SPB_16.05415.05415.3 4.5458 0.2675 100.0 2280.4443 2279.57 6629.2 1 6.059 48.5 2 R.KLDEMDKEKWNLQESKEK.Y * Jamie_Mitco_SPB_12.04261.04261.2 3.6683 0.2356 100.0 1747.3322 1745.8467 4826.7 1 4.593 76.9 1 R.REKEELNENSNNIR.I * Jamie_Mitco_SPB_11.03873.03873.2 3.597 0.4509 100.0 1588.2322 1589.6592 3782.7 1 7.156 83.3 3 R.EKEELNENSNNIR.I * Jamie_Mitco_SPB_12.04023.04023.1 1.617 0.1699 100.0 1589.48 1589.6592 2691.1 1 3.552 50.0 1 R.EKEELNENSNNIR.I * Jamie_Mitco_SPB_11.03771.03771.3 2.9548 0.0945 95.2 1592.1543 1589.6592 5211.3 5 3.7 43.8 2 R.EKEELNENSNNIR.I * Jamie_Mitco_SPB_9.03195.03195.1 1.4051 0.0543 95.7 1024.03 1024.2385 4107.7 160 2.762 50.0 1 R.IMEDKMTR.I * Jamie_Mitco_SPB_23.07980.07980.2 2.5832 0.1058 99.6 2065.3123 2066.3188 8054.0 1 3.827 46.9 1 R.IKKNYLSEITSLQEENR.R * Jamie_Mitco_SPB_28.09989.09989.3 4.6682 0.2875 100.0 2206.8542 2205.4473 8691.7 1 5.824 45.6 1 R.RKDNDSTMQLNDIISYYK.L * Jamie_Mitco_SPB_30.10709.10709.2 3.3462 0.0513 99.8 2050.392 2049.2598 7072.8 1 3.69 65.6 1 R.KDNDSTMQLNDIISYYK.L * Jamie_Mitco_SPB_38.13491.13491.2 3.588 0.491 100.0 1919.5922 1921.0858 8483.0 1 9.065 66.7 1 K.DNDSTMQLNDIISYYK.L * Jamie_Mitco_SPB_20.07125.07125.2 5.287 0.5181 100.0 2053.3323 2054.1777 8614.5 1 9.499 76.5 3 R.KGEHSLNISLPDDDELDR.D * Jamie_Mitco_SPB_21.07431.07431.3 2.6926 0.0634 90.1 2054.2144 2054.1777 5396.8 2 3.152 41.2 1 R.KGEHSLNISLPDDDELDR.D * Jamie_Mitco_SPB_27.09414.09414.3 4.4227 0.5085 100.0 3350.0044 3353.5425 11097.3 1 9.046 31.5 2 R.KGEHSLNISLPDDDELDRDYYNSHVYTR.Y * Jamie_Mitco_SPB_27.09489.09489.4 6.8306 0.35 100.0 3351.9365 3353.5425 4694.2 1 7.339 35.8 1 R.KGEHSLNISLPDDDELDRDYYNSHVYTR.Y * Jamie_Mitco_SPB_27.09461.09461.3 3.1526 0.2282 84.6 2273.9343 2274.3215 6951.9 2 4.81 38.2 1 L.PDDDELDRDYYNSHVYTR.Y * Jamie_Mitco_SPB_13.04655.04655.1 1.8731 0.3763 100.0 1317.28 1318.388 4277.7 1 6.231 61.1 1 R.DYYNSHVYTR.Y YBR109C 12 14 74.1% 147 16135 4.3 U CMD1 SGDID:S000000313, Chr II from 458356-457913, reverse complement, Verified ORF, "Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin" * Jamie_Mitco_SPB_52.18414.18414.2 2.6028 0.3664 100.0 3159.9922 3161.378 9037.4 1 6.7 25.0 1 R.SLGLSPSEAEVNDLMNEIDVDGNHQIEFS.E * Jamie_Mitco_SPB_65.23317.23317.3 4.4402 0.3902 100.0 4107.9546 4109.526 7741.1 1 7.196 25.0 1 R.SLGLSPSEAEVNDLMNEIDVDGNHQIEFSEFLALMSR.Q * Jamie_Mitco_SPB_24.08571.08571.2 2.8172 0.3135 100.0 1880.8121 1880.0618 5100.9 1 5.553 53.3 1 R.QLKSNDSEQELLEAFK.V * Jamie_Mitco_SPB_29.10086.10086.2 3.321 0.4207 100.0 1509.2322 1510.5975 7942.7 1 8.202 79.2 2 K.SNDSEQELLEAFK.V * Jamie_Mitco_SPB_28.09976.09976.1 2.48 0.1944 100.0 1509.37 1510.5975 6920.4 4 5.018 50.0 1 K.SNDSEQELLEAFK.V * Jamie_Mitco_SPB_49.17427.17427.3 4.5889 0.2757 100.0 3170.9343 3169.4692 8505.9 1 4.882 31.2 2 K.SNDSEQELLEAFKVFDKNGDGLISAAELK.H * Jamie_Mitco_SPB_20.06978.06978.2 2.654 0.2691 96.9 1578.8922 1578.7625 7789.2 1 5.178 60.7 1 V.FDKNGDGLISAAELK.H * Jamie_Mitco_SPB_33.11687.11687.3 3.5084 0.2748 100.0 2245.2844 2243.537 8053.4 1 5.482 34.2 1 K.HVLTSIGEKLTDAEVDDMLR.E * Jamie_Mitco_SPB_48.17157.17157.3 3.9315 0.2695 100.0 3471.8643 3472.803 7394.0 1 5.692 23.4 1 K.HVLTSIGEKLTDAEVDDMLREVSDGSGEINIQ.Q * Jamie_Mitco_SPB_61.21731.21731.3 2.6696 0.2017 98.1 4331.0044 4331.839 8500.8 42 3.629 17.3 1 K.HVLTSIGEKLTDAEVDDMLREVSDGSGEINIQQFAALLSK.- * Jamie_Mitco_SPB_25.08739.08739.2 4.4912 0.384 100.0 1794.3922 1792.999 8699.1 1 7.066 73.3 1 T.SIGEKLTDAEVDDMLR.E * Jamie_Mitco_SPB_51.18267.18267.3 4.5617 0.4607 100.0 3366.5645 3368.651 8671.6 1 6.97 30.0 1 T.SIGEKLTDAEVDDMLREVSDGSGEINIQQFA.A YAL047C 32 34 69.8% 622 72105 5.1 U SPC72 SGDID:S000000045, Chr I from 56858-54990, reverse complement, Verified ORF, "Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them to microtubules; has roles in astral microtubule formation and stabilization" * Jamie_Mitco_SPB_33.11681.11681.2 3.3522 0.5322 100.0 2934.872 2935.9753 7105.6 1 9.493 48.1 1 R.NNDNTGDDDDSEFTNSMDSGMSIPSLR.D * Jamie_Mitco_SPB_35.12384.12384.2 3.1872 0.331 100.0 3015.0723 3015.9753 6059.2 1 6.878 40.4 1 R.NNDNTGDDDDSEFTNS*MDSGMSIPSLR.D * Jamie_Mitco_SPB_32.11419.11419.3 3.3769 0.2138 100.0 3628.6443 3627.7324 8987.2 1 4.823 22.7 1 R.NNDNTGDDDDSEFTNSMDSGMSIPSLRDSMTTR.S * Jamie_Mitco_SPB_102.36557.36557.2 1.7497 0.0108 84.9 2166.9722 2168.2444 6210.6 76 3.155 33.3 1 R.RIPNKDGNAPS*LGNDTDFR.N * Jamie_Mitco_SPB_88.31609.31609.4 4.8549 0.3412 100.0 6086.9365 6088.5854 8233.1 1 5.267 15.7 1 K.EVEYQDTLQFVQENLENSESIVNTINHLLSFILTHFNEQDENAHLLDKEER.E * Jamie_Mitco_SPB_16.05634.05634.2 2.7107 0.419 100.0 2450.5322 2452.6426 7061.5 1 6.154 47.4 1 F.ILTHFNEQDENAHLLDKEER.E * Jamie_Mitco_SPB_52.18423.18423.3 3.4373 0.2691 100.0 3332.9343 3332.6553 10543.7 1 5.148 25.0 1 L.DKEERETLEETLELSSDYVLEKMDTLSK.F * Jamie_Mitco_SPB_43.15321.15321.2 3.9627 0.1618 100.0 2001.1122 1999.1761 9133.8 1 4.682 59.4 1 R.ETLEETLELSSDYVLEK.M * Jamie_Mitco_SPB_20.07083.07083.3 3.1164 0.1125 93.8 2903.7844 2904.0305 9359.4 128 3.128 21.0 1 R.AESKQDKEEFLSLAQSS*PAGSQLESR.D * Jamie_Mitco_SPB_29.10253.10253.2 3.7002 0.3078 100.0 2118.0522 2117.1907 7060.9 1 5.145 50.0 1 K.EEFLSLAQSS*PAGSQLESR.D * Jamie_Mitco_SPB_26.09281.09281.3 4.0369 0.3083 100.0 4405.344 4405.534 9932.6 119 6.427 15.4 1 K.EENTDGGYQNDEIHDSNNHIDTENVMANSTSLPISAVESR.F * Jamie_Mitco_SPB_21.07230.07230.2 2.9491 0.3271 100.0 1464.1721 1463.6738 5223.3 1 5.938 62.5 1 T.SLPISAVESRFEK.T * Jamie_Mitco_SPB_69.24603.24603.4 4.1914 0.2889 90.9 3753.6565 3753.1174 5688.5 28 4.971 20.2 1 S.RFEKT*LDT*QLEIVIEILHKEYDQFINSIR.L * Jamie_Mitco_SPB_49.17547.17547.3 3.6869 0.0634 95.2 2332.0444 2330.5542 4478.0 5 4.275 38.2 1 R.FEKT*LDT*QLEIVIEILHK.E * Jamie_Mitco_SPB_67.24019.24019.4 5.1519 0.1006 95.8 3440.4966 3436.93 7783.1 4 4.307 24.1 1 R.FEKTLDTQLEIVIEILHKEYDQFINSIR.L * Jamie_Mitco_SPB_76.27054.27054.3 3.6683 0.1563 99.5 3114.8342 3112.4636 7275.3 1 4.326 31.2 1 K.TLDT*QLEIVIEILHKEYDQFINSIR.L * Jamie_Mitco_SPB_30.10483.10483.2 4.9808 0.3898 100.0 2615.612 2614.823 8709.4 1 6.729 50.0 1 K.LNEQSHLLDSLELEENSSSVIEK.Q * Jamie_Mitco_SPB_35.12281.12281.4 4.5613 0.1842 100.0 3934.0566 3935.3342 8731.8 41 3.722 19.2 1 K.LNEQSHLLDSLELEENSSSVIEKQDHLISQLKEK.I * Jamie_Mitco_SPB_19.06839.06839.2 3.5241 0.4174 100.0 1486.5521 1487.6934 7554.1 1 7.615 83.3 1 K.IESQSVLINNLEK.L * Jamie_Mitco_SPB_19.06492.06492.2 3.4493 0.1806 100.0 1457.2122 1457.7104 5404.7 1 5.325 86.4 1 I.NNLEKLKEDIIK.M * Jamie_Mitco_SPB_28.09730.09730.2 5.0887 0.4689 100.0 1881.2322 1882.0367 9034.1 1 8.568 75.0 2 K.LKENNWDSYINDLEK.Q * Jamie_Mitco_SPB_17.05905.05905.1 2.079 0.1708 100.0 1586.79 1587.7294 6162.1 105 4.093 41.7 1 K.SEEFHVIQNQLDK.L * Jamie_Mitco_SPB_17.05859.05859.3 3.2688 0.1989 100.0 1587.0243 1587.7294 5733.5 97 4.272 43.8 1 K.SEEFHVIQNQLDK.L * Jamie_Mitco_SPB_37.13302.13302.2 3.5719 0.3465 100.0 2703.5122 2704.9963 9546.5 1 8.311 50.0 1 K.SEEFHVIQNQLDKLDLENYQLK.N * Jamie_Mitco_SPB_12.04099.04099.1 2.6424 0.1981 100.0 1187.92 1188.2828 3887.1 1 5.275 77.8 1 K.NQLNTLDNQK.L * Jamie_Mitco_SPB_79.28175.28175.3 3.0269 0.2279 99.5 3606.2644 3608.2183 7685.8 15 4.442 19.8 1 K.LILSQYESNFIKFNQNLLLHLDSIFNILQK.I * Jamie_Mitco_SPB_19.06663.06663.2 2.7246 0.347 100.0 1406.7722 1407.5663 6883.5 1 6.022 72.7 1 K.ILQESSIAQFDR.K * Jamie_Mitco_SPB_103.36771.36771.3 5.3993 0.4219 100.0 5042.034 5040.6045 7764.9 1 7.676 21.6 1 K.LESLYTFIETALESIINSYISSLISMETPEQPHQQGNELTATPNK.E * Jamie_Mitco_SPB_103.36757.36757.4 3.3008 0.1682 94.2 5735.0566 5733.3315 9214.5 1 3.556 15.0 1 K.LESLYTFIETALESIINSYISSLISMETPEQPHQQGNELTAT*PNKELTLR.I * Jamie_Mitco_SPB_13.04337.04337.1 2.5375 0.3056 100.0 1189.46 1189.2676 3839.1 1 5.578 72.2 1 K.ALEQENESLR.S * Jamie_Mitco_SPB_11.03801.03801.2 3.3015 0.2371 100.0 1405.1322 1404.5199 6879.3 2 4.937 72.7 2 K.ALEQENESLRSK.L * Jamie_Mitco_SPB_34.12075.12075.1 1.6157 0.1802 100.0 1326.43 1327.4368 3021.4 7 4.05 65.0 1 R.SKLFNLS*INNP.- YNL225C 61 75 68.0% 581 67400 6.0 U CNM67 SGDID:S000005169, Chr XIV from 224470-222725, reverse complement, Verified ORF, "Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration" * Jamie_Mitco_SPB_35.12363.12363.2 2.5872 0.3268 100.0 1668.6921 1669.8517 8195.7 1 6.091 62.5 1 M.TDFDLMNFPFHER.L * Jamie_Mitco_SPB_28.09783.09783.3 4.5124 0.2735 100.0 3297.5344 3297.4758 4624.1 205 5.494 24.1 1 R.LDSPVS*ENGEIKDGEPIPQNWLNENHVGK.S * Jamie_Mitco_SPB_31.10941.10941.3 4.9121 0.445 100.0 3377.3342 3377.4758 3914.7 1 7.173 31.2 1 R.LDS*PVS*ENGEIKDGEPIPQNWLNENHVGK.S * Jamie_Mitco_SPB_50.17793.17793.2 4.0972 0.3937 100.0 2420.9521 2420.6936 9688.4 1 7.087 47.5 2 K.SILPLFVNPEDVINCNFSNAR.D * Jamie_Mitco_SPB_55.19761.19761.2 4.7459 0.4511 100.0 2500.0122 2500.6936 6670.3 1 8.106 57.5 2 K.SILPLFVNPEDVINCNFS*NAR.D * Jamie_Mitco_SPB_30.10533.10533.2 3.6662 0.1438 94.9 1897.6721 1897.0203 8482.6 1 5.241 63.3 1 L.FVNPEDVINCNFSNAR.D * Jamie_Mitco_SPB_15.05121.05121.3 5.0904 0.4637 100.0 2164.7944 2166.2983 9782.5 1 7.802 44.1 1 R.DSYEENKSPSMDQMNYAR.N * Jamie_Mitco_SPB_15.05116.05116.2 3.5749 0.402 100.0 2165.2322 2166.2983 6455.3 1 6.863 55.9 1 R.DSYEENKSPSMDQMNYAR.N * Jamie_Mitco_SPB_15.05313.05313.2 2.3379 0.1453 99.8 1381.6122 1380.4468 6691.4 1 4.104 65.0 1 K.SPS*MDQMNYAR.N * Jamie_Mitco_SPB_13.04326.04326.2 3.2781 0.4484 100.0 1734.3722 1734.8632 6100.4 1 7.194 67.9 1 R.NTSYQESPGLQERPK.N * Jamie_Mitco_SPB_13.04509.04509.3 2.068 0.2075 95.5 1815.5944 1814.8632 5007.4 27 5.134 39.3 1 R.NTS*YQESPGLQERPK.N * Jamie_Mitco_SPB_8.02752.02752.2 1.4495 0.017 80.6 1569.5322 1568.7269 7521.5 253 3.26 38.5 1 K.NEKDKSPIGTDVHK.K * Jamie_Mitco_SPB_6.01833.01833.2 2.4897 0.1901 100.0 1695.9521 1696.901 6610.0 3 4.597 53.6 1 K.NEKDKSPIGTDVHKK.D * Jamie_Mitco_SPB_8.02697.02697.3 2.1036 0.1115 89.6 1776.5044 1776.901 4097.4 6 3.884 41.1 1 K.NEKDKS*PIGTDVHKK.D * Jamie_Mitco_SPB_7.02278.02278.2 2.7129 0.2878 100.0 1197.5322 1197.3335 5435.8 7 5.642 65.0 1 K.DKSPIGTDVHK.K * Jamie_Mitco_SPB_7.02227.02227.3 2.7785 0.1667 99.5 1199.1843 1197.3335 4696.4 12 3.678 50.0 1 K.DKSPIGTDVHK.K * Jamie_Mitco_SPB_1.00014.00014.2 2.833 0.2637 100.0 1276.9122 1277.3335 4097.5 1 5.317 70.0 2 K.DKS*PIGTDVHK.K * Jamie_Mitco_SPB_1.00008.00008.3 2.5977 0.1646 98.2 1405.6444 1405.5076 6789.0 1 4.48 47.7 1 K.DKS*PIGTDVHKK.D * Jamie_Mitco_SPB_16.05445.05445.1 2.2221 0.3047 100.0 1282.47 1283.4294 3856.1 3 6.073 60.0 1 K.DVPNFIHSTPR.E * Jamie_Mitco_SPB_17.05890.05890.1 1.4681 0.1639 100.0 1362.44 1363.4294 2641.5 88 4.27 45.0 1 K.DVPNFIHST*PR.E * Jamie_Mitco_SPB_14.04918.04918.3 3.6407 0.2327 100.0 2885.3643 2886.9226 7649.9 1 4.757 27.9 1 R.ANEQASAQPTDEHTSPDISIEDCNGAK.I * Jamie_Mitco_SPB_17.06016.06016.2 1.5836 0.0595 89.1 1051.4122 1050.2432 5276.4 187 2.962 62.5 1 K.IFLQNSLSK.E * Jamie_Mitco_SPB_21.07271.07271.2 1.7847 0.0429 89.4 1597.0521 1597.8113 7931.8 25 3.82 41.7 1 K.IFLQNSLSKEDFR.M * Jamie_Mitco_SPB_20.06951.06951.3 3.1553 0.3789 100.0 1599.2344 1597.8113 3317.8 1 7.845 45.8 2 K.IFLQNSLSKEDFR.M * Jamie_Mitco_SPB_14.04905.04905.2 2.5842 0.1911 100.0 1185.0122 1185.3689 6124.6 210 4.343 61.1 1 K.VIELGRDNLR.Q * Jamie_Mitco_SPB_17.05831.05831.2 3.2678 0.1565 94.6 1334.5721 1332.4967 4343.2 1 4.396 81.8 1 A.NSLQKELEAATK.S * Jamie_Mitco_SPB_25.08967.08967.3 5.5364 0.4489 100.0 2801.1843 2803.1826 7222.2 1 7.784 33.7 1 K.SNDKTLDNKKKIEEQTVLIENLTK.D * Jamie_Mitco_SPB_21.07455.07455.3 2.7423 0.2663 82.1 2487.4143 2486.912 8400.3 158 4.574 22.5 1 D.KTLDNKKKIEEQTVLIENLTK.D * Jamie_Mitco_SPB_21.07560.07560.2 4.7431 0.1921 100.0 1787.7722 1787.1069 9193.8 1 7.016 78.6 4 K.KKIEEQTVLIENLTK.D * Jamie_Mitco_SPB_40.14362.14362.4 3.6611 0.1562 96.0 3089.5764 3088.6277 8404.6 4 3.813 24.7 1 K.KKIEEQTVLIENLTKDLSLNKEMLEK.A * Jamie_Mitco_SPB_21.07455.07455.2 4.1728 0.2494 100.0 1658.6122 1658.9329 8400.3 1 6.611 80.8 1 K.KIEEQTVLIENLTK.D * Jamie_Mitco_SPB_40.14326.14326.4 5.0561 0.2676 100.0 2961.0166 2960.4536 11138.8 1 5.096 31.9 1 K.KIEEQTVLIENLTKDLSLNKEMLEK.A * Jamie_Mitco_SPB_26.09345.09345.2 4.3002 0.3129 100.0 1531.0922 1530.7588 7567.5 1 6.62 87.5 1 K.IEEQTVLIENLTK.D * Jamie_Mitco_SPB_45.16095.16095.4 3.5797 0.197 98.9 3702.9766 3704.225 6486.4 8 4.026 20.4 1 K.IEEQTVLIENLTKDLSLNKEMLEKANDTIQTK.H * Jamie_Mitco_SPB_17.06053.06053.2 2.4756 0.0824 99.4 1322.0521 1320.5438 5978.3 2 3.595 70.0 1 K.DLSLNKEMLEK.A * Jamie_Mitco_SPB_22.07845.07845.3 4.6588 0.2951 100.0 2191.2544 2192.4895 10370.7 1 6.429 37.5 2 K.DLSLNKEMLEKANDTIQTK.H * Jamie_Mitco_SPB_22.07786.07786.1 2.7529 0.4322 100.0 1326.55 1327.5236 4083.1 1 7.201 68.2 1 K.HTALLSLTDSLR.K * Jamie_Mitco_SPB_23.08062.08062.2 2.7179 0.5058 100.0 1326.7322 1327.5236 3299.4 1 8.636 77.3 2 K.HTALLSLTDSLR.K * Jamie_Mitco_SPB_66.23554.23554.2 4.3074 0.4726 100.0 2904.1921 2906.2585 9031.8 1 9.165 50.0 2 R.KAELFEIPIGILFFDLYDSEENSSK.L * Jamie_Mitco_SPB_67.23920.23920.3 5.7546 0.4865 100.0 2907.3542 2906.2585 10329.1 1 7.856 34.4 2 R.KAELFEIPIGILFFDLYDSEENSSK.L * Jamie_Mitco_SPB_64.22977.22977.4 4.8069 0.2059 100.0 3884.8564 3883.387 8948.4 1 5.246 24.5 2 R.KAELFEIPIGILFFDLYDSEENSSKLDHILQEK.Y * Jamie_Mitco_SPB_61.21732.21732.4 7.4616 0.5367 100.0 4498.177 4499.1167 9537.8 1 9.078 22.5 1 R.KAELFEIPIGILFFDLYDSEENSSKLDHILQEKYPNIK.G * Jamie_Mitco_SPB_74.26393.26393.2 3.4919 0.3714 100.0 2777.9722 2778.0845 12134.9 1 6.748 34.8 1 K.AELFEIPIGILFFDLYDSEENSSK.L * Jamie_Mitco_SPB_17.05826.05826.1 2.4408 0.3995 100.0 1524.49 1525.6245 3900.7 1 6.773 62.5 1 K.GFLCASQQEELSR.I * Jamie_Mitco_SPB_18.06361.06361.2 2.3274 0.0659 97.9 1527.0322 1525.6245 7227.7 3 3.284 62.5 1 K.GFLCASQQEELSR.I * Jamie_Mitco_SPB_22.07569.07569.4 3.0832 0.1625 94.4 2118.7366 2120.327 3930.9 1 4.048 40.2 1 R.FKNAKAEAEDLRNELENK.K * Jamie_Mitco_SPB_22.07744.07744.3 2.9989 0.211 99.4 2119.3743 2120.327 7342.1 1 4.231 39.7 1 R.FKNAKAEAEDLRNELENK.K * Jamie_Mitco_SPB_30.10750.10750.3 5.2365 0.3799 100.0 3118.5244 3120.551 5951.5 1 6.925 34.0 2 R.FKNAKAEAEDLRNELENKKIEIQTMR.E * Jamie_Mitco_SPB_15.05229.05229.2 3.7664 0.2703 100.0 1531.0122 1531.6195 6594.5 1 5.918 75.0 1 K.AEAEDLRNELENK.K * Jamie_Mitco_SPB_15.05157.05157.3 2.9848 0.2756 100.0 1532.8444 1531.6195 2756.6 1 4.774 50.0 1 K.AEAEDLRNELENK.K * Jamie_Mitco_SPB_23.07959.07959.4 3.6867 0.1459 96.0 2532.3364 2531.8438 3721.1 9 4.065 28.3 1 K.AEAEDLRNELENKKIEIQTMR.E * Jamie_Mitco_SPB_22.07669.07669.3 4.2517 0.1347 99.5 2535.0244 2531.8438 8298.7 1 4.0 33.8 2 K.AEAEDLRNELENKKIEIQTMR.E * Jamie_Mitco_SPB_8.02649.02649.2 2.3845 0.2402 100.0 1230.3322 1232.3794 3468.5 1 4.53 80.0 1 R.EKNNTLIGTNK.T * Jamie_Mitco_SPB_10.03405.03405.1 1.5022 0.0422 95.8 974.05 975.0898 4387.1 42 3.097 62.5 1 K.NNTLIGTNK.T * Jamie_Mitco_SPB_13.04475.04475.1 1.9154 0.0944 100.0 1038.99 1039.1829 4698.1 3 3.925 71.4 1 K.ILCDKFDK.L * Jamie_Mitco_SPB_11.03735.03735.2 2.2627 0.0392 96.2 1711.6522 1713.9255 6392.7 22 4.225 46.2 1 K.SKEKYETSLKDKEK.M * Jamie_Mitco_SPB_5.01474.01474.2 1.9881 0.0263 92.7 1241.9321 1241.3837 4300.9 7 3.716 72.2 1 K.YETSLKDKEK.M * Jamie_Mitco_SPB_14.04930.04930.2 2.4402 0.1105 99.8 1239.8322 1240.4167 3193.8 2 3.661 72.2 1 R.QVYSQEMNLK.K * Jamie_Mitco_SPB_25.08807.08807.2 3.4378 0.4087 100.0 1772.5922 1773.9575 7991.1 1 7.462 73.1 1 K.KFTDQAYYDFMSTR.R * Jamie_Mitco_SPB_13.04357.04357.1 1.4369 0.3392 100.0 1252.6 1253.3751 3848.4 3 5.732 50.0 1 R.MDSIDHHLER.C * Jamie_Mitco_SPB_20.07131.07131.2 2.9134 0.174 100.0 1555.9122 1556.7246 5240.1 1 4.333 86.4 1 R.CLDHLYDHILEK.M YPL124W 19 34 61.3% 253 29280 9.5 U SPC29 SGDID:S000006045, Chr XVI from 316754-317515, Verified ORF, "Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inner plaque component Spc110p; required for SPB duplication" * Jamie_Mitco_SPB_11.03939.03939.2 2.902 0.1387 100.0 1137.0721 1137.2377 5323.0 1 5.261 93.8 1 K.KFQDDTLNR.V * Jamie_Mitco_SPB_11.03903.03903.2 2.2549 0.0992 99.4 1217.7522 1217.2377 3666.0 1 3.186 87.5 1 K.KFQDDT*LNR.V * Jamie_Mitco_SPB_13.04587.04587.3 4.5115 0.2381 100.0 1954.9443 1954.1039 7145.4 1 4.823 50.0 1 K.LREENFSSNTSELGNKK.H * Jamie_Mitco_SPB_29.10108.10108.2 2.5427 0.3671 100.0 1839.9922 1839.0326 7979.8 1 5.575 50.0 1 R.LPPPPFS*SYGMPPTNR.S * Jamie_Mitco_SPB_24.08343.08343.2 3.4451 0.3985 100.0 1892.3121 1892.0758 9815.2 1 6.501 56.7 1 T.DKFASQNVIDDQRLEI.K * Jamie_Mitco_SPB_19.06581.06581.2 2.7598 0.0787 99.4 1778.4122 1776.9872 8696.8 6 3.628 46.4 1 K.FASQNVIDDQRLEIK.Y * Jamie_Mitco_SPB_24.08607.08607.2 2.9154 0.2847 100.0 1694.2922 1694.8821 9172.6 32 5.048 42.9 1 R.IVYDQGTVIDNLTSR.I * Jamie_Mitco_SPB_40.14199.14199.4 3.8239 0.1554 95.9 3106.6965 3107.4531 6128.0 1 3.624 25.0 1 R.ITRLESFILNSISDRGDKNFASLEHSR.S * Jamie_Mitco_SPB_17.06045.06045.1 1.27 0.0061 85.2 1064.11 1065.0844 2784.3 223 3.333 50.0 1 R.SFS*GFPTNK.T * Jamie_Mitco_SPB_31.10807.10807.2 3.1511 0.2559 100.0 2009.7122 2009.2588 8039.4 1 5.77 65.6 1 K.TYGLQMGGLYENDMPYR.R * Jamie_Mitco_SPB_25.08782.08782.2 3.0422 0.1533 100.0 2163.8523 2165.4463 6735.3 97 4.798 35.3 1 K.TYGLQMGGLYENDMPYRR.S * Jamie_Mitco_SPB_16.05704.05704.3 2.8014 0.0964 92.8 2323.2844 2324.377 7417.4 1 3.454 33.3 1 R.EDRSSQIHIENEST*EDILK.I * Jamie_Mitco_SPB_22.07749.07749.2 3.352 0.4643 100.0 1922.4521 1923.9855 5379.6 2 7.288 66.7 5 R.SSQIHIENES*TEDILK.I * Jamie_Mitco_SPB_18.06423.06423.3 4.055 0.0555 96.5 1924.2843 1923.9855 6958.4 1 3.894 48.3 2 R.SSQIHIENES*TEDILK.I * Jamie_Mitco_SPB_31.10872.10872.2 4.1396 0.3533 100.0 1924.7122 1923.9855 7243.5 1 6.098 56.7 11 R.SSQIHIENEST*EDILK.I * Jamie_Mitco_SPB_27.09550.09550.2 2.4369 0.2273 90.4 2035.5521 2037.1449 5457.4 1 5.255 56.2 1 R.SSQIHIENES*TEDILKI.L * Jamie_Mitco_SPB_38.13395.13395.2 3.2085 0.3482 100.0 2149.112 2150.3042 6478.8 1 6.252 58.8 1 R.SSQIHIENEST*EDILKIL.S * Jamie_Mitco_SPB_39.14022.14022.2 2.5078 0.2439 95.6 2323.3123 2324.4607 7216.9 2 5.239 39.5 1 R.SSQIHIENES*TEDILKILSS.S * Jamie_Mitco_SPB_42.14935.14935.2 2.1135 0.2151 99.8 2889.0723 2889.9604 5547.4 104 4.108 26.1 1 R.SSQIHIENES*TEDILKILSSS*FHN.- YLR212C 14 15 47.4% 473 52628 4.7 U TUB4 SGDID:S000004202, Chr XII from 566283-564862, reverse complement, Verified ORF, "Gamma-tubulin, involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body" * Jamie_Mitco_SPB_25.08949.08949.1 1.8483 0.1498 100.0 993.43 993.19366 10187.2 42 3.79 50.0 1 K.FLWSQLAK.E * Jamie_Mitco_SPB_23.07960.07960.3 2.7806 0.1415 95.1 2175.4143 2174.113 3189.5 212 3.631 25.0 1 K.EHAIGTDGLSQLPDS*S*TER.D * Jamie_Mitco_SPB_22.07731.07731.3 3.1683 0.155 97.3 3136.6743 3136.3152 6747.0 1 5.087 24.1 1 K.EHAIGTDGLSQLPDSSTERDDDTKPFFR.E * Jamie_Mitco_SPB_20.07120.07120.3 3.2897 0.3221 100.0 3621.8643 3622.8003 6963.8 105 5.4 20.2 1 K.EHAIGTDGLSQLPDSSTERDDDTKPFFRENSR.N * Jamie_Mitco_SPB_27.09408.09408.2 2.3996 0.272 100.0 2471.9321 2471.5615 8249.6 3 5.155 32.6 1 R.NTWVASDGASAGNSWANGYDIGTR.N * Jamie_Mitco_SPB_18.06385.06385.2 3.3766 0.4139 100.0 1899.6921 1899.928 6542.3 1 7.787 58.3 1 A.SDGASAGNSWANGYDIGTR.N * Jamie_Mitco_SPB_58.20790.20790.4 5.2481 0.222 100.0 5468.5366 5467.9326 11042.7 1 4.97 18.0 1 R.NQDDILNKIDKEIDSTDNFEGFQLLHSVAGGTGSGLGSNLLEALCDRYPK.K * Jamie_Mitco_SPB_41.14637.14637.2 4.5492 0.411 100.0 2323.1921 2322.5742 7773.4 1 6.265 50.0 1 R.LIEDSDATVVFDNASLLNISGK.V * Jamie_Mitco_SPB_69.24660.24660.3 3.5574 0.3149 100.0 4659.324 4661.1714 8340.8 1 5.467 18.3 1 K.GHSSYDVMLDLLDPSNSLVSTAMNNPTYFNVYNTIIGNVEPR.Q * Jamie_Mitco_SPB_73.26070.26070.3 5.3688 0.3936 100.0 4962.804 4960.224 7168.1 1 5.754 21.5 2 R.EVVQSLMEDYVAAEQDSYLDDVLVDDENMVGELEEDLDADGDHK.L * Jamie_Mitco_SPB_75.26751.26751.3 4.6218 0.4993 100.0 5171.0645 5172.516 7620.5 1 8.938 19.4 1 R.EVVQSLMEDYVAAEQDSYLDDVLVDDENMVGELEEDLDADGDHKLV.- * Jamie_Mitco_SPB_72.25594.25594.3 5.1501 0.5729 100.0 4715.7544 4717.005 6477.2 1 9.403 23.2 1 Q.SLMEDYVAAEQDSYLDDVLVDDENMVGELEEDLDADGDHKLV.- * Jamie_Mitco_SPB_70.24995.24995.3 6.4677 0.455 100.0 4630.1343 4629.927 8300.1 1 8.155 25.0 1 S.LMEDYVAAEQDSYLDDVLVDDENMVGELEEDLDADGDHKLV.- * Jamie_Mitco_SPB_68.24318.24318.3 5.8657 0.545 100.0 4515.9243 4516.767 8292.1 1 8.436 24.4 1 L.MEDYVAAEQDSYLDDVLVDDENMVGELEEDLDADGDHKLV.- contaminant_KERATIN02 22 30 45.2% 622 61987 5.2 U no description * Jamie_Mitco_SPB_11.03682.03682.2 3.793 0.3104 100.0 1234.3922 1233.2833 6092.5 1 6.929 66.7 1 T.SGGGGGGGLGSGGSIR.S * Jamie_Mitco_SPB_10.03298.03298.2 2.772 0.3198 100.0 1237.5721 1236.2401 7201.8 1 6.003 70.8 2 R.FSSSSGYGGGSSR.V * Jamie_Mitco_SPB_29.10336.10336.2 5.2197 0.5256 100.0 2706.2322 2706.7605 9672.9 1 11.258 40.3 1 R.GGGGSFGYSYGGGSGGGFSASSLGGGFGGGSR.G * Jamie_Mitco_SPB_11.03796.03796.1 1.5506 0.0331 95.9 1065.11 1066.1742 3417.2 186 3.46 50.0 1 K.STMQELNSR.L * Jamie_Mitco_SPB_12.04083.04083.2 2.3721 0.3027 100.0 1065.6322 1066.1742 4368.3 5 6.432 81.2 1 K.STMQELNSR.L * Jamie_Mitco_SPB_54.19265.19265.3 3.8619 0.1871 100.0 3949.7944 3946.3167 9025.4 5 5.204 19.7 1 K.NYSPYYNTIDDLKDQIVDLTVGNNKTLLDIDNTR.M * Jamie_Mitco_SPB_17.05898.05898.1 1.9814 0.1261 100.0 1060.42 1061.1802 5423.4 1 4.589 68.8 1 K.TLLDIDNTR.M * Jamie_Mitco_SPB_17.06054.06054.2 1.8074 0.1433 98.7 1062.0922 1061.1802 5733.2 14 3.682 68.8 1 K.TLLDIDNTR.M * Jamie_Mitco_SPB_19.06526.06526.2 2.1286 0.2711 100.0 898.5722 898.02155 4909.5 1 5.469 83.3 1 R.MTLDDFR.I * Jamie_Mitco_SPB_50.17689.17689.2 2.7378 0.1324 99.8 2172.632 2172.4702 7961.9 1 3.914 52.9 1 K.SDLEMQYETLQEELMALK.K * Jamie_Mitco_SPB_42.14784.14784.2 1.6579 0.018 83.4 2458.0723 2460.6443 8152.5 45 2.613 27.8 1 K.S*DLEMQYET*LQEELMALKK.N * Jamie_Mitco_SPB_29.10240.10240.2 2.1732 0.1454 99.0 1851.6522 1853.1003 8606.8 69 4.195 32.1 1 K.TLNDMRQEYEQLIAK.N * Jamie_Mitco_SPB_28.09822.09822.3 3.719 0.4058 100.0 1854.6543 1853.1003 2711.1 1 5.733 44.6 1 K.TLNDMRQEYEQLIAK.N * Jamie_Mitco_SPB_42.14807.14807.4 5.5966 0.1687 100.0 4395.2563 4391.7095 9973.0 2 4.526 19.4 2 K.NRKDIENQYETQITQIEHEVSSSGQEVQSSAKEVTQLR.H * Jamie_Mitco_SPB_41.14526.14526.2 4.6206 0.5334 100.0 3265.4521 3266.413 8817.6 1 9.683 37.5 1 K.DIENQYETQITQIEHEVSSSGQEVQSSAK.E * Jamie_Mitco_SPB_43.15251.15251.3 4.1279 0.2729 100.0 3266.8442 3266.413 6294.5 1 5.178 25.0 1 K.DIENQYETQITQIEHEVSSSGQEVQSSAK.E * Jamie_Mitco_SPB_31.11045.11045.2 4.0604 0.3436 100.0 1838.4722 1839.0557 9729.9 1 8.411 66.7 3 R.HGVQELEIELQSQLSK.K * Jamie_Mitco_SPB_26.09348.09348.2 2.6167 0.3611 100.0 1966.4122 1967.2297 8155.2 1 5.888 43.8 1 R.HGVQELEIELQSQLSKK.A * Jamie_Mitco_SPB_28.09852.09852.3 2.9086 0.1271 95.5 1967.0343 1967.2297 7004.9 70 4.187 35.9 1 R.HGVQELEIELQSQLSKK.A * Jamie_Mitco_SPB_7.02385.02385.4 3.1872 0.079 80.1 3583.5764 3585.4917 7536.1 41 2.678 16.2 1 K.IGLGGRGGS*GGSY*GRGSRGGSGGSYGGGGSGGGYGGGSGSR.G * Jamie_Mitco_SPB_7.02385.02385.2 5.3267 0.3773 100.0 1792.2922 1792.7324 7536.1 1 10.488 59.1 5 R.GGSGGSYGGGGSGGGYGGGSGSR.G * Jamie_Mitco_SPB_121.43494.43494.3 4.4658 0.2592 100.0 3227.4543 3225.1118 8794.3 1 5.713 22.4 1 R.GGSGGSHGGGSGFGGESGGSYGGGEEASGSGGGYGGGSGK.S YJR053W 23 24 44.9% 574 66087 5.9 U BFA1 SGDID:S000003814, Chr X from 533941-535665, Verified ORF, "Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pathways that control exit from mitosis" * Jamie_Mitco_SPB_41.14502.14502.2 3.0166 0.4165 100.0 3178.8323 3180.2068 8553.2 1 7.612 36.0 2 K.QADDDQDMEVDQDDEFLNDFQEFQNK.K * Jamie_Mitco_SPB_23.07989.07989.2 1.8035 0.0331 90.2 1255.5122 1256.3141 8110.6 67 2.997 61.1 1 K.NDIFAEEFDR.K * Jamie_Mitco_SPB_12.04155.04155.2 1.9718 0.0103 92.7 960.0122 958.1026 4387.7 10 2.805 78.6 1 K.LSLEDKPR.L * Jamie_Mitco_SPB_33.11883.11883.2 2.3625 0.2356 100.0 1477.5721 1476.691 3775.4 1 4.787 58.3 1 K.S*MPNLALVNPAIR.E * Jamie_Mitco_SPB_15.05071.05071.2 2.9124 0.2064 100.0 1428.8121 1427.465 7548.1 5 4.681 72.7 1 K.IDNDT*QDTILAK.F * Jamie_Mitco_SPB_52.18570.18570.4 4.8529 0.3946 100.0 4298.8965 4299.3423 9748.4 1 5.875 21.6 1 A.KFS*SDDEGDFLTGFEELEGEAIDETISSNDKESADHPR.F * Jamie_Mitco_SPB_57.20320.20320.3 3.3996 0.1868 99.5 4174.224 4171.1685 5844.1 1 4.189 20.8 1 K.FSS*DDEGDFLTGFEELEGEAIDETISSNDKESADHPR.F * Jamie_Mitco_SPB_57.20236.20236.4 4.4811 0.1491 96.6 4175.7363 4171.1685 6996.7 1 3.84 19.4 1 K.FSS*DDEGDFLTGFEELEGEAIDETISSNDKESADHPR.F * Jamie_Mitco_SPB_16.05703.05703.3 3.4852 0.2734 100.0 2672.4844 2672.7327 8924.7 103 5.354 22.8 1 F.EELEGEAIDETISSNDKESADHPR.F * Jamie_Mitco_SPB_14.05001.05001.3 2.2139 0.0798 89.6 1367.1543 1368.449 2153.2 12 4.381 40.0 1 K.HDELLT*PGLHR.R * Jamie_Mitco_SPB_25.08740.08740.1 2.7291 0.0928 100.0 1512.45 1511.5878 5654.6 1 3.814 68.2 1 R.DWNTQQELDSFK.E * Jamie_Mitco_SPB_21.07234.07234.2 2.4247 0.1652 99.8 1850.3722 1848.8773 2854.2 63 4.236 50.0 1 R.DWNTQQELDS*FKEK.R * Jamie_Mitco_SPB_10.03507.03507.2 4.0635 0.1275 100.0 1649.4521 1647.6677 5332.1 1 4.808 69.2 1 R.HCS*NQNVQLNGPAK.I * Jamie_Mitco_SPB_5.01524.01524.2 2.0681 0.1796 99.8 1153.0521 1153.237 6635.1 1 4.957 72.2 1 K.FSDVDTANRK.A * Jamie_Mitco_SPB_20.07169.07169.2 4.8991 0.41 100.0 1885.5322 1886.1766 8716.3 1 8.852 83.3 1 R.NQKVIGNMILDEQNLR.W * Jamie_Mitco_SPB_24.08313.08313.2 3.4414 0.2878 100.0 1516.0922 1515.768 7168.9 1 6.517 70.8 1 K.VIGNMILDEQNLR.W * Jamie_Mitco_SPB_54.19311.19311.2 2.065 0.0439 93.0 2761.372 2762.002 8457.4 1 4.159 31.2 1 R.WVSVS*EEEADPFAGIPEINLPPVGK.S * Jamie_Mitco_SPB_42.14943.14943.2 2.7346 0.2758 100.0 2397.2922 2396.6562 8641.7 1 5.07 36.4 1 V.SVSEEEADPFAGIPEINLPPVGK.S * Jamie_Mitco_SPB_22.07771.07771.2 2.2396 0.2904 100.0 2178.1921 2179.3481 9540.3 1 5.73 36.8 1 R.TLNGTTETPEISSTFHLSSR.A * Jamie_Mitco_SPB_34.11977.11977.2 1.3667 0.0406 80.8 1595.3121 1592.8932 6451.1 25 3.034 45.5 1 R.WCKKLASWFIPR.D * Jamie_Mitco_SPB_35.12274.12274.2 3.6033 0.4685 100.0 2355.9922 2356.5146 8126.5 1 7.414 40.0 1 R.DETIISVDEETIMDESTVNSK.R * Jamie_Mitco_SPB_11.03630.03630.1 1.5587 0.258 100.0 1021.18 1022.16144 3858.9 22 4.65 56.2 1 R.NMVINSTKD.- * Jamie_Mitco_SPB_11.03875.03875.2 1.8796 0.1576 99.4 1022.39215 1022.16144 5444.8 329 4.418 56.2 1 R.NMVINSTKD.- YDR382W 4 4 44.5% 110 11050 4.1 U RPP2B SGDID:S000002790, Chr IV from 1239482-1239814, Verified ORF, "Ribosomal protein P2 beta, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm" * Jamie_Mitco_SPB_35.12451.12451.3 2.4587 0.1839 95.2 4815.9546 4817.9277 5037.1 7 4.348 15.1 1 K.KFATVPTGGASSAAAGAAGAAAGGDAAEEEKEEEAKEES*DDDMGFGLFD.- * Jamie_Mitco_SPB_37.12979.12979.3 4.7039 0.2813 100.0 4610.574 4609.7534 8256.4 1 5.887 18.1 1 K.FATVPTGGASSAAAGAAGAAAGGDAAEEEKEEEAKEESDDDMGFGLFD.- * Jamie_Mitco_SPB_40.14153.14153.3 4.2052 0.3934 100.0 4687.8545 4689.7534 5961.1 1 6.819 19.7 1 K.FATVPTGGASSAAAGAAGAAAGGDAAEEEKEEEAKEES*DDDMGFGLFD.- * Jamie_Mitco_SPB_34.11925.11925.3 4.3814 0.2106 100.0 3642.6543 3642.621 8731.8 1 4.932 22.1 1 A.AAGAAGAAAGGDAAEEEKEEEAKEES*DDDMGFGLFD.- YOR373W 52 57 43.6% 851 94104 7.0 U NUD1 SGDID:S000005900, Chr XV from 1036830-1039385, Verified ORF, "Component of the spindle pole body outer plaque, required for exit from mitosis" * Jamie_Mitco_SPB_28.09840.09840.4 3.1665 0.0899 82.1 4411.6167 4411.569 6326.2 263 2.862 16.2 1 R.SNAHSNSGKVFKEYESNHDFQDSNFT*SQVVEPAISDSVK.K * Jamie_Mitco_SPB_29.10284.10284.2 2.6205 0.3029 100.0 3074.132 3074.1975 7736.8 2 6.953 26.9 1 K.EYESNHDFQDSNFTSQVVEPAISDSVK.K * Jamie_Mitco_SPB_15.05145.05145.3 3.2037 0.1981 100.0 2038.6444 2039.2708 8551.3 6 4.611 37.5 1 K.KPPTMTVLNNYST*VHQK.V * Jamie_Mitco_SPB_13.04593.04593.2 2.4811 0.2926 96.8 1577.2722 1577.6476 4507.2 1 5.319 56.7 1 K.VPSGFSGTTATSHQEA.Q * Jamie_Mitco_SPB_15.05290.05290.3 3.5203 0.4163 100.0 2019.9243 2020.1655 4592.7 1 6.291 34.7 1 K.VPSGFSGTTATSHQEAQWK.Q * Jamie_Mitco_SPB_122.43590.43590.2 2.644 0.0674 98.7 2020.6921 2020.1655 7848.5 74 4.736 36.1 2 K.VPSGFSGTTATSHQEAQWK.Q * Jamie_Mitco_SPB_39.13973.13973.3 2.9804 0.2168 99.5 3557.1843 3553.9507 5982.1 1 4.35 21.4 1 K.QYFPGIGSGGGTNFGGAVGTANKVPESDLIVSDLVK.D * Jamie_Mitco_SPB_59.21028.21028.2 3.4465 0.4068 100.0 2621.372 2622.8865 3329.3 1 7.085 43.5 1 T.ANKVPESDLIVSDLVKDLSGVLET*.N * Jamie_Mitco_SPB_34.11967.11967.2 2.9822 0.0812 99.8 1405.8322 1404.4735 5541.0 1 4.409 81.8 2 K.DLS*GVLETNTFK.R * Jamie_Mitco_SPB_47.16691.16691.3 2.9551 0.0679 90.8 2945.1843 2942.8909 5204.1 97 3.146 22.1 1 K.VSSSFS*DDS*DSGPAAEAHDVFDGILQK.Q * Jamie_Mitco_SPB_25.08881.08881.2 2.453 0.2099 91.7 1668.8522 1668.8467 6868.1 33 3.948 40.0 1 S.GPAAEAHDVFDGILQK.Q * Jamie_Mitco_SPB_10.03247.03247.2 3.7281 0.1719 100.0 2173.3123 2172.152 5904.3 1 6.405 55.6 2 K.NNNNNNNNNNNNSININNK.D * Jamie_Mitco_SPB_12.04135.04135.2 3.9838 0.1367 100.0 2254.3123 2252.152 5544.3 1 5.085 52.8 1 K.NNNNNNNNNNNNS*ININNK.D * Jamie_Mitco_SPB_11.03827.03827.3 3.4924 0.0881 94.1 2707.3442 2708.6106 6013.4 68 3.698 28.4 1 K.NNNNNNNNNNNNS*ININNKDNAR.T * Jamie_Mitco_SPB_19.06567.06567.3 4.6199 0.4327 100.0 2377.4644 2378.6523 6287.6 1 7.206 41.7 1 K.KPPLNTISPGQLGYQFNHTHGA.W * Jamie_Mitco_SPB_10.03520.03520.2 1.9926 0.1897 99.8 870.1722 872.05255 5319.9 2 4.139 85.7 1 K.APSILDKK.A * Jamie_Mitco_SPB_16.05501.05501.2 1.8592 0.0075 86.8 1736.1721 1737.8204 6644.6 364 3.528 35.7 1 K.AY*ELTSTKPGDVGYR.Q * Jamie_Mitco_SPB_16.05536.05536.2 2.8656 0.3739 100.0 2336.892 2338.401 6692.9 2 6.199 36.8 1 K.KIQEEENLANSDDTPLDT*PK.F * Jamie_Mitco_SPB_16.05512.05512.2 3.0909 0.3192 100.0 2418.892 2418.401 7802.7 1 5.798 39.5 1 K.KIQEEENLANSDDT*PLDT*PK.F * Jamie_Mitco_SPB_29.10332.10332.3 4.402 0.3143 100.0 3125.0044 3124.385 8926.2 1 5.775 29.8 1 K.KIQEEENLANSDDTPLDTPKFNDLFTK.N * Jamie_Mitco_SPB_30.10801.10801.3 4.5483 0.271 100.0 3205.1943 3204.385 10611.4 1 4.745 30.8 1 K.KIQEEENLANSDDTPLDT*PKFNDLFTK.N * Jamie_Mitco_SPB_34.12013.12013.3 4.0271 0.1735 100.0 3285.9844 3284.385 4331.6 1 4.778 26.9 1 K.KIQEEENLANS*DDT*PLDTPKFNDLFTK.N * Jamie_Mitco_SPB_32.11257.11257.3 3.5875 0.1354 98.1 3286.0745 3284.385 5992.1 1 4.273 27.9 1 K.KIQEEENLANSDDT*PLDT*PKFNDLFTK.N * Jamie_Mitco_SPB_28.10042.10042.3 2.576 0.0717 86.5 3712.9443 3712.8333 5403.8 22 3.736 19.2 1 K.KIQEEENLANSDDT*PLDT*PKFNDLFTKNGTR.A * Jamie_Mitco_SPB_18.06333.06333.2 2.8527 0.1876 100.0 2209.872 2210.2268 8832.1 1 5.608 47.2 2 K.IQEEENLANSDDTPLDT*PK.F * Jamie_Mitco_SPB_18.06361.06361.3 3.5142 0.2498 100.0 2290.0444 2290.2268 7227.7 1 4.648 38.9 1 K.IQEEENLANS*DDTPLDT*PK.F * Jamie_Mitco_SPB_18.06363.06363.2 4.9124 0.3809 100.0 2292.0723 2290.2268 9303.0 1 6.456 58.3 2 K.IQEEENLANSDDT*PLDT*PK.F * Jamie_Mitco_SPB_35.12289.12289.2 2.7352 0.0512 98.7 3078.672 3076.211 7012.4 1 3.288 32.0 1 K.IQEEENLANSDDT*PLDTPKFNDLFTK.N * Jamie_Mitco_SPB_37.13237.13237.2 2.5208 0.2962 100.0 3154.112 3156.211 7646.1 1 5.909 28.0 1 K.IQEEENLANS*DDTPLDT*PKFNDLFTK.N * Jamie_Mitco_SPB_38.13329.13329.3 5.0641 0.4224 100.0 3154.9744 3156.211 10449.5 1 6.767 29.0 1 K.IQEEENLANSDDT*PLDT*PKFNDLFTK.N * Jamie_Mitco_SPB_30.10479.10479.4 4.2769 0.2764 100.0 3425.3364 3424.6592 7279.9 11 5.64 23.0 1 K.IQEEENLANSDDTPLDTPKFNDLFTKNGTR.A * Jamie_Mitco_SPB_13.04648.04648.2 2.5019 0.2477 100.0 1313.9122 1313.4532 5914.3 128 4.455 54.5 1 R.SISNSNLLEAHK.K * Jamie_Mitco_SPB_15.05273.05273.2 2.1739 0.0563 96.7 1394.1122 1393.4532 3298.5 3 3.132 63.6 1 R.SIS*NSNLLEAHK.K * Jamie_Mitco_SPB_13.04569.04569.3 1.9043 0.0806 80.0 1521.7144 1521.6273 3583.2 9 3.258 47.9 1 R.SIS*NSNLLEAHKK.L * Jamie_Mitco_SPB_30.10477.10477.3 3.3528 0.0459 91.7 3166.7043 3166.3794 7292.2 7 4.187 23.1 1 K.LKTFPAERVEDITS*ISEVNTSFNETEK.Q * Jamie_Mitco_SPB_52.18611.18611.4 5.0915 0.2695 100.0 4154.0566 4150.5835 6659.5 1 5.507 22.4 1 K.LKTFPAERVEDITSISEVNTS*FNETEKQLISILTSK.L * Jamie_Mitco_SPB_57.20201.20201.3 3.6501 0.1121 96.6 3829.5842 3829.25 8876.2 6 4.118 18.2 1 K.TFPAERVEDITSISEVNTSFNETEKQLISILTSK.L * Jamie_Mitco_SPB_60.21276.21276.3 3.729 0.1318 99.0 3987.6843 3989.25 4624.5 1 4.543 20.5 1 K.TFPAERVEDITSIS*EVNTS*FNETEKQLISILTSK.L * Jamie_Mitco_SPB_121.43372.43372.2 2.3755 0.1176 99.0 2225.4321 2223.2659 9382.5 6 3.978 36.1 1 R.VEDITSISEVNT*SFNETEK.Q * Jamie_Mitco_SPB_30.10773.10773.2 3.3687 0.3121 100.0 2301.912 2303.2659 7225.5 2 6.681 41.7 1 R.VEDITSIS*EVNTS*FNETEK.Q * Jamie_Mitco_SPB_21.07227.07227.1 1.876 0.0205 96.5 1001.92 1003.22723 2489.6 18 3.171 68.8 1 K.QLISILTSK.L * Jamie_Mitco_SPB_57.20418.20418.2 1.9637 0.0582 92.7 2537.4922 2535.724 9489.7 4 3.43 28.6 1 K.QLISILTSKLSGSPS*YDSDWEK.I * Jamie_Mitco_SPB_16.05412.05412.1 2.711 0.2539 100.0 1470.3 1471.52 5547.5 1 5.459 66.7 1 K.LSGSPSYDSDWEK.I * Jamie_Mitco_SPB_17.05983.05983.1 2.2776 0.3611 100.0 1550.83 1551.52 3400.7 2 5.536 54.2 1 K.LSGS*PSYDSDWEK.I * Jamie_Mitco_SPB_31.10977.10977.2 1.8686 0.1271 97.5 1904.0322 1906.013 5418.9 74 3.705 40.0 1 K.LSGS*PSYDSDWEKILK.V * Jamie_Mitco_SPB_30.10608.10608.2 2.5457 0.1599 99.8 2277.112 2276.5535 9822.8 4 3.759 38.2 1 K.LQNYKLDDQFTFPYQNLK.I * Jamie_Mitco_SPB_30.10684.10684.2 3.7134 0.3092 100.0 1630.5322 1629.8094 6716.0 1 6.005 70.8 1 K.LDDQFTFPYQNLK.I * Jamie_Mitco_SPB_50.17819.17819.2 2.1013 0.0151 90.5 2137.7522 2137.4868 10211.2 23 3.755 28.9 1 K.WPATLQILEINGGLASSLPR.F * Jamie_Mitco_SPB_22.07716.07716.1 2.0475 0.2113 100.0 1271.91 1272.4435 5514.0 3 5.175 55.0 1 R.DFTHLPVDLSK.E * Jamie_Mitco_SPB_22.07804.07804.2 2.6252 0.3023 100.0 1272.5521 1272.4435 6888.8 1 4.795 70.0 1 R.DFTHLPVDLSK.E * Jamie_Mitco_SPB_10.03276.03276.1 2.041 0.2846 100.0 912.34 913.02136 3758.7 3 5.402 64.3 1 R.NNPITTPR.H * Jamie_Mitco_SPB_10.03317.03317.2 1.5015 0.0819 89.9 912.47217 913.02136 4223.2 139 3.227 57.1 1 R.NNPITTPR.H YLR457C 14 14 40.8% 319 37354 10.2 U NBP1 SGDID:S000004449, Chr XII from 1056768-1055809, reverse complement, Verified ORF, "Component of the mitotic apparatus containing a coiled-coil domain, essential for the G2/M transition" * Jamie_Mitco_SPB_14.04947.04947.2 1.9839 0.1407 99.4 1070.1522 1068.1283 5336.1 5 4.501 62.5 1 R.EYGSLDEVR.K * Jamie_Mitco_SPB_26.09083.09083.3 3.1922 0.2169 100.0 2159.3342 2159.3784 7023.0 1 4.91 36.8 1 K.IFQTIRDVFSNDNENMSK.M * Jamie_Mitco_SPB_14.04798.04798.2 2.0856 0.1276 99.8 1011.09216 1009.1069 5111.0 152 4.204 64.3 1 R.NQSLYLDR.E * Jamie_Mitco_SPB_19.06729.06729.2 3.2346 0.233 100.0 2086.672 2085.3245 7432.6 2 5.328 46.9 1 S.KLQSLQEALNYSNEKYR.I * Jamie_Mitco_SPB_21.07378.07378.2 2.5697 0.0866 98.9 1956.3121 1957.1503 6170.5 322 4.08 33.3 1 K.LQSLQEALNYSNEKYR.I * Jamie_Mitco_SPB_29.10441.10441.2 2.7214 0.328 100.0 2025.0322 2026.1644 5330.5 1 5.16 62.5 1 R.ILEDLLDSSNIHPS*YTK.S * Jamie_Mitco_SPB_30.10780.10780.2 2.6823 0.0194 97.5 2028.0521 2026.1644 6313.3 28 3.232 40.6 1 R.ILEDLLDSS*NIHPSYTK.S * Jamie_Mitco_SPB_34.12208.12208.2 3.0349 0.3396 100.0 2106.7522 2106.1643 4772.4 3 5.914 46.9 1 R.ILEDLLDS*S*NIHPSYTK.S * Jamie_Mitco_SPB_36.12795.12795.2 2.2917 0.116 98.9 2106.8323 2106.1643 7468.5 13 5.049 37.5 1 R.ILEDLLDSS*NIHPSY*TK.S * Jamie_Mitco_SPB_6.02022.02022.1 1.6987 0.2128 100.0 792.69 792.92865 3493.1 9 4.205 66.7 1 R.TMSNLAR.E * Jamie_Mitco_SPB_22.07879.07879.3 3.1418 0.2303 100.0 2383.3145 2381.562 4269.1 3 4.409 33.3 1 R.ENDEIKPLKIDLS*PS*PIRR.T * Jamie_Mitco_SPB_20.06889.06889.2 2.0964 0.152 99.8 1294.2322 1293.3911 5703.1 11 4.108 65.0 1 R.TNS*LFTSSPMK.T * Jamie_Mitco_SPB_33.11719.11719.2 2.009 0.1639 98.8 2808.112 2805.999 6800.2 3 3.769 30.4 1 R.DGNIPEMQPLQENISPACPT*PPYR.S * Jamie_Mitco_SPB_37.13252.13252.2 1.9138 0.0808 94.1 2886.172 2885.999 6264.4 1 4.075 32.6 1 R.DGNIPEMQPLQENIS*PACPT*PPYR.S YPL255W 12 12 39.5% 385 45384 6.5 U BBP1 SGDID:S000006176, Chr XVI from 67725-68882, Verified ORF, "Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periphery; forms a complex with a nuclear envelope protein Mps2p and SPB components Spc29p and Kar1p; required for mitotic functions of Cdc5p" * Jamie_Mitco_SPB_24.08466.08466.2 1.6669 0.0207 84.4 1798.8522 1798.0637 7592.2 347 3.439 30.0 1 K.WTKDALFGTDISPSMK.Y * Jamie_Mitco_SPB_32.11502.11502.1 2.373 0.2667 100.0 1462.5 1462.5714 3030.9 9 4.9 54.2 1 K.DALFGTDIS*PSMK.Y * Jamie_Mitco_SPB_27.09670.09670.2 2.1044 0.0785 96.5 1754.4922 1753.9214 7038.0 10 3.302 46.4 1 K.DALFGTDISPS*MKYK.D * Jamie_Mitco_SPB_77.27400.27400.4 3.818 0.1757 96.7 4769.9766 4770.895 6812.5 78 3.542 16.2 1 R.SRYAQDDTNFS*MKFGNDSNRRSTNLSRS*NSWSGLDSTLHR.K * Jamie_Mitco_SPB_13.04583.04583.2 2.8856 0.3405 100.0 1291.2522 1291.4496 5865.0 1 5.731 80.0 1 K.LFNKDGVNEVR.R * Jamie_Mitco_SPB_16.05543.05543.2 2.4328 0.1406 99.8 1978.2122 1979.1112 4412.0 202 3.204 34.4 1 K.KVLDQTISHEAELATS*R.E * Jamie_Mitco_SPB_17.06063.06063.2 3.1215 0.3762 100.0 1850.0721 1850.9371 3252.6 1 5.827 66.7 1 K.VLDQTISHEAELATS*R.E * Jamie_Mitco_SPB_27.09642.09642.2 4.8343 0.4785 100.0 2011.0122 2012.2671 10395.6 1 8.27 66.7 1 R.LYQEEDLKNFEIQTLK.Q * Jamie_Mitco_SPB_25.08878.08878.3 3.999 0.3492 100.0 2338.5544 2338.6814 6004.4 1 5.454 36.1 1 R.LSDLELKYTNLQIEKDMQR.D * Jamie_Mitco_SPB_54.19161.19161.3 4.1677 0.1413 100.0 2058.6243 2059.2834 8245.5 25 4.592 40.6 1 R.DNYESEIHDLLLQLSLR.N * Jamie_Mitco_SPB_55.19709.19709.2 2.2444 0.1627 99.8 2059.4321 2059.2834 7777.2 4 4.236 40.6 1 R.DNYESEIHDLLLQLSLR.N * Jamie_Mitco_SPB_21.07284.07284.2 2.7148 0.2538 100.0 1574.0322 1573.4839 4906.9 1 5.174 65.4 1 R.KDTS*AGS*NIFSTGQ.- contaminant_KERATIN22 19 19 37.8% 645 65865 8.0 U no description * Jamie_Mitco_SPB_68.24265.24265.2 2.2591 0.2879 95.0 1312.5922 1313.375 3824.3 2 4.983 53.6 1 R.GRGGGGGGFRGFSSG.S * Jamie_Mitco_SPB_13.04488.04488.1 2.7101 0.3776 100.0 1255.47 1255.3298 2977.2 1 6.98 53.8 1 R.GFSSGSAVVSGGSR.R * Jamie_Mitco_SPB_13.04584.04584.1 2.896 0.4298 100.0 1320.09 1321.3542 4215.5 1 7.648 53.3 1 R.HGGGGGGFGGGGFGSR.S * Jamie_Mitco_SPB_13.04588.04588.2 4.8131 0.3416 100.0 1322.0122 1321.3542 4416.8 1 9.606 86.7 1 R.HGGGGGGFGGGGFGSR.S * Jamie_Mitco_SPB_44.15508.15508.4 4.4927 0.1939 100.0 5117.6167 5117.7324 10201.3 2 4.312 14.1 1 R.FGGFGGPGGVGGLGGPGGFGPGGYPGGIHEVSVNQSLLQPLNVKVDPEIQNVK.A Jamie_Mitco_SPB_15.05274.05274.1 2.4248 0.2961 100.0 1475.37 1476.6726 5059.3 3 5.303 54.5 1 R.FLEQQNQVLQTK.W Jamie_Mitco_SPB_16.05519.05519.2 3.8565 0.1292 100.0 1475.9722 1476.6726 6149.3 1 6.512 86.4 1 R.FLEQQNQVLQTK.W * Jamie_Mitco_SPB_24.08632.08632.3 3.9415 0.3797 100.0 2256.2944 2257.4338 6320.8 1 5.646 34.7 1 R.TSQNSELNNMQDLVEDYKK.K Jamie_Mitco_SPB_11.03904.03904.2 2.7247 0.1669 95.5 1195.0122 1195.3176 4778.7 1 5.748 93.8 1 K.KYEDEINKR.T Jamie_Mitco_SPB_9.03005.03005.2 2.13 0.144 99.8 1067.3722 1067.1436 6192.7 305 4.211 64.3 1 K.YEDEINKR.T * Jamie_Mitco_SPB_60.21303.21303.3 4.2409 0.2558 100.0 3628.9443 3629.9783 8311.9 1 5.397 19.2 1 T.NVILS*MDNSRNLDLDSIIAEVKAQYEEIAQR.S Jamie_Mitco_SPB_40.14340.14340.1 2.6538 0.1875 100.0 1330.02 1330.5211 5956.2 2 6.415 59.1 1 R.NLDLDSIIAEVK.A Jamie_Mitco_SPB_60.21303.21303.2 5.3215 0.5087 100.0 2419.632 2419.694 8311.9 1 8.061 60.0 1 R.NLDLDSIIAEVKAQYEEIAQR.S * Jamie_Mitco_SPB_12.04203.04203.2 2.2792 0.1789 99.8 1391.2322 1392.5083 3254.5 2 4.909 59.1 1 R.SKEEAEALYHSK.Y * Jamie_Mitco_SPB_8.02746.02746.2 2.202 0.1344 99.8 1027.3322 1027.1655 5671.4 2 3.789 75.0 1 R.HGDSLKEIK.I Jamie_Mitco_SPB_15.05271.05271.2 2.1131 0.0396 97.5 972.9322 974.102 5671.0 66 3.969 78.6 1 K.IEISELNR.V * Jamie_Mitco_SPB_16.05532.05532.2 2.8074 0.2347 100.0 1329.1122 1330.3971 5794.3 1 6.544 72.7 1 K.NVQDAIADAEQR.G * Jamie_Mitco_SPB_38.13505.13505.2 4.7206 0.4178 100.0 2198.132 2199.4258 7282.9 1 7.894 58.3 1 R.NKLNDLEEALQQAKEDLAR.L * Jamie_Mitco_SPB_9.03075.03075.2 2.2507 0.3675 100.0 1739.3722 1741.6818 5680.9 1 6.113 57.9 1 R.GSSSGGGYSSGSSSYGSGGR.Q YLR146W-A 4 4 35.5% 62 7078 4.8 U YLR146W-A SGDID:S000113566, Chr XII from 433871-434059, Uncharacterized ORF, "Putative protein of unknown function" * Jamie_Mitco_SPB_43.15424.15424.2 4.4966 0.3383 100.0 1887.9722 1889.2261 5588.3 1 7.097 64.3 1 A.RMLQNEIQQLFAQLR.D * Jamie_Mitco_SPB_50.17754.17754.2 2.1222 0.0464 94.2 1732.9122 1733.0386 8429.7 8 2.812 53.8 1 R.MLQNEIQQLFAQLR.D * Jamie_Mitco_SPB_13.04629.04629.3 3.5717 0.2189 100.0 1449.6843 1449.6096 4354.1 1 5.199 56.8 1 L.FAQLRDTNSQIR.C * Jamie_Mitco_SPB_3.00802.00802.2 2.6691 0.312 100.0 1102.3522 1103.2235 6721.0 1 5.638 81.2 1 Q.LRDTNSQIR.C YPL131W 8 8 35.0% 297 33715 6.8 U RPL5 SGDID:S000006052, Chr XVI from 303120-304013, Verified ORF, "Protein component of the large (60S) ribosomal subunit with similarity to E. coli L18 and rat L5 ribosomal proteins; binds 5S rRNA and is required for 60S subunit assembly" * Jamie_Mitco_SPB_44.15789.15789.4 4.8127 0.3929 100.0 3601.3364 3599.927 5886.9 1 6.616 26.3 1 K.LGLDETYKGVEEVEGEYELTEAVEDGPRPFKV.F * Jamie_Mitco_SPB_55.19704.19704.4 3.5495 0.1155 87.7 4543.2163 4543.0405 9129.8 30 3.116 16.7 1 K.LGLDETYKGVEEVEGEYELTEAVEDGPRPFKVFLDIGLQR.T * Jamie_Mitco_SPB_30.10612.10612.2 2.2194 0.0843 97.4 2209.0522 2208.297 6890.7 4 3.97 34.2 1 K.GVEEVEGEYELTEAVEDGPR.P * Jamie_Mitco_SPB_33.11873.11873.2 4.4106 0.2807 100.0 2582.3523 2580.7644 7045.3 1 6.061 54.5 1 K.GVEEVEGEYELTEAVEDGPRPFK.V * Jamie_Mitco_SPB_36.12881.12881.2 3.9587 0.2015 100.0 2593.7722 2591.7217 11047.9 1 5.734 52.4 1 R.SYIFGGHVSQYMEELADDDEER.F * Jamie_Mitco_SPB_55.19497.19497.3 4.9515 0.2405 100.0 3344.6042 3343.602 9811.6 1 5.197 26.9 1 R.SYIFGGHVSQYMEELADDDEERFSELFK.G * Jamie_Mitco_SPB_41.14715.14715.2 3.2627 0.4307 100.0 2653.412 2654.8035 7701.8 1 6.354 30.4 1 K.GYLADDIDADSLEDIYTSAHEAIR.A * Jamie_Mitco_SPB_11.03898.03898.2 2.3797 0.1524 99.8 1429.0322 1430.6006 6601.4 13 4.449 59.1 1 K.KFTKEQYAAESK.K YOL039W 3 4 34.9% 106 10746 4.0 U RPP2A SGDID:S000005399, Chr XV from 254295-254615, Verified ORF, "Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm" * Jamie_Mitco_SPB_29.10330.10330.2 2.7879 0.3367 100.0 1545.9321 1545.727 6008.0 1 5.821 69.2 2 K.AILESVGIEIEDEK.V * Jamie_Mitco_SPB_51.18142.18142.2 4.6631 0.4406 100.0 2615.912 2616.9658 10358.5 1 9.344 39.6 1 K.AILESVGIEIEDEKVSSVLSALEGK.S * Jamie_Mitco_SPB_15.05400.05400.1 2.4629 0.2902 100.0 1333.3 1334.4229 6132.4 1 6.236 54.5 1 K.SVDELITEGNEK.L contaminant_KERATIN03 17 21 34.2% 593 59519 5.2 U no description * Jamie_Mitco_SPB_23.07998.07998.2 2.5855 0.1203 99.8 1711.3722 1708.7844 6524.6 2 4.307 38.9 2 K.GSLGGGFSSGGFSGGSFSR.G * Jamie_Mitco_SPB_14.05020.05020.1 2.8907 0.2264 100.0 1383.08 1382.4668 6962.2 1 4.933 68.2 2 R.ALEESNYELEGK.I * Jamie_Mitco_SPB_14.04962.04962.2 1.6851 0.0019 82.6 2094.912 2096.2693 5551.3 62 2.713 37.5 1 K.IKEWYEKHGNSHQGEPR.D * Jamie_Mitco_SPB_57.20244.20244.2 3.7383 0.3024 100.0 3055.7122 3054.4277 8494.7 1 5.72 34.6 2 K.TIDDLKNQILNLTTDNANILLQIDNAR.L * Jamie_Mitco_SPB_43.15456.15456.2 3.5562 0.1551 100.0 2368.672 2368.6523 7205.9 2 5.916 40.0 1 K.NQILNLTTDNANILLQIDNAR.L * Jamie_Mitco_SPB_14.05009.05009.2 2.3779 0.2985 100.0 1235.2322 1235.4258 4403.1 2 5.533 77.8 1 R.LKYENEVALR.Q * Jamie_Mitco_SPB_15.05278.05278.3 1.9773 0.1621 92.8 1357.7344 1358.4972 3934.8 1 4.229 50.0 1 R.QSVEADINGLRR.V * Jamie_Mitco_SPB_49.17418.17418.2 5.0001 0.48 100.0 2873.892 2874.2134 5471.2 1 8.746 44.2 1 R.NVSTGDVNVEMNAAPGVDLTQLLNNMR.S * Jamie_Mitco_SPB_27.09389.09389.3 3.5236 0.2252 100.0 2587.1042 2585.749 7073.3 1 4.287 40.0 1 R.SQYEQLAEQNRKDAEAWFNEK.S * Jamie_Mitco_SPB_19.06831.06831.1 2.1131 0.23 100.0 1109.48 1110.1681 5387.2 1 5.928 68.8 1 K.DAEAWFNEK.S * Jamie_Mitco_SPB_29.10137.10137.3 5.3473 0.2838 100.0 2213.3643 2213.4033 6584.8 2 5.649 41.7 1 K.SKELTTEIDNNIEQISSYK.S * Jamie_Mitco_SPB_29.10110.10110.2 6.2202 0.4826 100.0 2213.652 2213.4033 7917.8 1 9.903 75.0 2 K.SKELTTEIDNNIEQISSYK.S * Jamie_Mitco_SPB_35.12497.12497.3 4.0581 0.2999 100.0 3197.6643 3198.5115 9576.7 1 6.19 26.0 1 K.SKELTTEIDNNIEQISSYKSEITELRR.N * Jamie_Mitco_SPB_34.12035.12035.3 3.017 0.018 84.6 3203.0044 3202.324 9530.2 258 2.596 21.0 1 K.SKELTTEIDNNIEQISS*Y*KSEITELR.R * Jamie_Mitco_SPB_34.12047.12047.2 3.2802 0.4098 100.0 1998.3922 1998.151 6466.3 1 6.629 56.2 1 K.ELTTEIDNNIEQISSYK.S * Jamie_Mitco_SPB_26.09126.09126.2 4.2023 0.4534 100.0 2083.112 2084.2104 9769.9 1 8.027 68.8 1 R.AETECQNTEYQQLLDIK.I * Jamie_Mitco_SPB_13.04443.04443.2 3.622 0.4606 100.0 1263.6322 1263.3066 7507.1 1 7.603 76.9 1 R.SLLEGEGSSGGGGR.G contaminant_KERATIN13 25 30 28.9% 643 65494 6.6 U no description * Jamie_Mitco_SPB_14.04799.04799.2 2.0013 0.2649 90.6 1001.1322 1000.0586 5150.9 11 4.532 55.0 1 G.SFGAGGGFGSR.S * Jamie_Mitco_SPB_26.09187.09187.1 2.7442 0.1414 100.0 1384.51 1384.5315 6003.3 230 4.518 45.5 1 K.SLNNQFASFIDK.V * Jamie_Mitco_SPB_31.10813.10813.2 3.4903 0.3939 100.0 1638.6921 1639.8516 8285.1 1 6.844 50.0 2 K.SLNNQFASFIDKVR.F Jamie_Mitco_SPB_15.05274.05274.1 2.4248 0.2961 100.0 1475.37 1476.6726 5059.3 3 5.303 54.5 1 R.FLEQQNQVLQTK.W Jamie_Mitco_SPB_16.05519.05519.2 3.8565 0.1292 100.0 1475.9722 1476.6726 6149.3 1 6.512 86.4 1 R.FLEQQNQVLQTK.W * Jamie_Mitco_SPB_35.12323.12323.3 2.7406 0.1035 93.1 2934.5344 2934.2786 9582.4 245 3.639 21.7 1 R.FLEQQNQVLQTKWELLQQVDTSTR.T * Jamie_Mitco_SPB_25.08997.08997.2 3.0191 0.3506 100.0 1475.0922 1476.6293 10193.1 1 6.849 77.3 1 K.WELLQQVDTSTR.T * Jamie_Mitco_SPB_25.08874.08874.1 1.3602 0.0054 86.3 1476.69 1476.6293 4249.1 24 2.747 40.9 1 K.WELLQQVDTSTR.T * Jamie_Mitco_SPB_43.15165.15165.2 2.0537 0.1944 99.8 1993.5721 1995.2017 7955.8 1 4.311 46.7 1 R.THNLEPYFESFINNLR.R * Jamie_Mitco_SPB_43.15213.15213.3 2.7596 0.0181 84.8 1995.8344 1995.2017 8312.8 102 3.514 33.3 1 R.THNLEPYFESFINNLR.R Jamie_Mitco_SPB_11.03904.03904.2 2.7247 0.1669 95.5 1195.0122 1195.3176 4778.7 1 5.748 93.8 1 N.KYEDEINKR.T Jamie_Mitco_SPB_9.03005.03005.2 2.13 0.144 99.8 1067.3722 1067.1436 6192.7 305 4.211 64.3 1 K.YEDEINKR.T * Jamie_Mitco_SPB_13.04662.04662.2 1.9844 0.0165 90.4 1394.4722 1394.5675 4879.3 216 3.323 45.5 1 R.TNAENEFVTIKK.D * Jamie_Mitco_SPB_26.09137.09137.2 2.5017 0.116 99.6 2376.612 2375.6558 7398.5 1 3.939 40.0 1 R.TNAENEFVTIKKDVDGAYMTK.V * Jamie_Mitco_SPB_84.30142.30142.4 6.2117 0.411 100.0 4532.2964 4530.035 10936.6 1 7.455 21.1 1 K.LDNLQQEIDFLTALYQAELSQMQTQISETNVILSMDNNR.Q * Jamie_Mitco_SPB_12.04131.04131.2 4.0043 0.4301 100.0 1341.0721 1341.4607 6180.8 1 7.437 86.4 1 K.SKAEAESLYQSK.Y * Jamie_Mitco_SPB_12.04151.04151.1 2.7592 0.1076 100.0 1342.2 1341.4607 5738.6 2 4.709 63.6 1 K.SKAEAESLYQSK.Y * Jamie_Mitco_SPB_20.07155.07155.3 6.2786 0.4406 100.0 2503.3442 2502.7405 6005.6 1 9.057 44.0 3 K.SKAEAESLYQSKYEELQITAGR.H * Jamie_Mitco_SPB_21.07398.07398.2 2.3667 0.0317 96.2 2504.3523 2502.7405 7826.4 3 3.528 40.5 2 K.SKAEAESLYQSKYEELQITAGR.H * Jamie_Mitco_SPB_21.07539.07539.2 3.1684 0.3649 100.0 2285.412 2287.4883 8058.3 1 6.939 42.1 2 K.AEAESLYQSKYEELQITAGR.H * Jamie_Mitco_SPB_16.05469.05469.2 3.7617 0.2505 100.0 1180.6921 1180.303 7887.6 1 7.769 94.4 1 K.YEELQITAGR.H Jamie_Mitco_SPB_15.05271.05271.2 2.1131 0.0396 97.5 972.9322 974.102 5671.0 66 3.969 78.6 1 K.IEISELNR.V * Jamie_Mitco_SPB_10.03579.03579.2 1.9605 0.0651 96.3 1201.6322 1202.3964 6722.7 8 3.352 66.7 1 R.LRSEIDNVKK.Q * Jamie_Mitco_SPB_19.06565.06565.2 2.0436 0.1311 98.7 1523.7922 1524.7754 6232.4 26 4.049 54.5 1 R.LLRDYQELMNTK.L * Jamie_Mitco_SPB_16.05529.05529.2 1.3955 0.0745 86.6 1140.8922 1142.2689 3935.4 413 3.121 62.5 1 R.DYQELMNTK.L YHL043W 2 2 25.9% 170 19812 8.7 U ECM34 SGDID:S000001035, Chr VIII from 14899-15411, Verified ORF, "Non-essential protein of unknown function" * Jamie_Mitco_SPB_6.01973.01973.1 1.5306 0.2177 83.3 850.01 849.96814 2759.7 28 4.33 57.1 1 V.FSGIGGRR.P * Jamie_Mitco_SPB_88.31398.31398.4 3.0627 0.1226 85.0 4504.8965 4507.1357 8757.6 20 3.164 17.6 1 K.HKRNPGFLRRHS*TIVILVISLAVSFSWEAFKMY*RER.T YLR150W 3 3 23.4% 273 29995 9.7 U STM1 SGDID:S000004140, Chr XII from 440468-441289, Verified ORF, "Protein that binds G4 quadruplex and purine motif triplex nucleic acid; acts with Cdc13p to maintain telomere structure; interacts with ribosomes and subtelomeric Y' DNA; multicopy suppressor of tom1 and pop2 mutations" * Jamie_Mitco_SPB_14.04864.04864.2 3.4954 0.336 100.0 1887.2922 1887.9952 5642.6 1 7.61 61.1 1 A.AEIAEDAAEAEDAGKPKTA.Q * Jamie_Mitco_SPB_29.10349.10349.3 4.4312 0.316 100.0 2865.1443 2864.1443 7410.4 1 5.638 35.4 1 K.TAQLSLQDYLNQQANNQFNKVPEAK.K * Jamie_Mitco_SPB_20.06970.06970.3 4.9137 0.3997 100.0 2492.5144 2491.801 10088.7 1 6.922 34.5 1 K.KVELDAERIETAEKEAYVPATK.V YGL075C 5 6 22.0% 387 44585 8.3 U MPS2 SGDID:S000003043, Chr VII from 368091-366928, reverse complement, Verified ORF, "Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required for insertion of the newly duplicated SPB into the nuclear envelope; potentially phosphorylated by Cdc28p" * Jamie_Mitco_SPB_66.23598.23598.2 3.4292 0.1937 100.0 3118.7722 3118.4395 6834.8 1 4.983 35.2 1 R.EILENAFIGVDSIPSDFIVSMNLNS*PSK.F * Jamie_Mitco_SPB_9.02997.02997.2 2.1436 0.2497 100.0 1424.2922 1425.4551 4972.2 5 4.488 62.5 1 K.NKNTEGAGIST*PR.K * Jamie_Mitco_SPB_12.04065.04065.2 1.9493 0.0211 89.6 1667.8322 1666.7904 5318.7 4 3.116 50.0 1 R.KYVYAHDTPSQNSR.L * Jamie_Mitco_SPB_39.13927.13927.2 2.0226 0.1518 98.9 1661.3322 1661.8981 7860.2 87 3.532 42.3 1 R.LQLSWWENSGILSK.I * Jamie_Mitco_SPB_77.27623.27623.2 2.2016 0.1909 99.8 2092.0122 2092.269 6840.3 6 4.206 40.0 2 K.IVWFFEDQTDLETEYR.S YLL045C 3 3 21.1% 256 28112 10.0 U RPL8B SGDID:S000003968, Chr XII from 48628-47858, reverse complement, Verified ORF, "Ribosomal protein L4 of the large (60S) ribosomal subunit, nearly identical to Rpl8Ap and has similarity to rat L7a ribosomal protein; mutation results in decreased amounts of free 60S subunits" * Jamie_Mitco_SPB_70.25153.25153.2 2.0706 0.1406 98.7 2196.0723 2196.5298 8365.2 28 3.398 30.0 1 -.MAPGKKVAPAPFGAKST*KSNK.A * Jamie_Mitco_SPB_24.08565.08565.2 1.8656 0.0061 87.9 1486.1522 1486.711 5917.0 77 3.402 45.8 1 K.YGLNHVVSLIENK.K * Jamie_Mitco_SPB_24.08328.08328.2 3.1719 0.2206 100.0 2016.2722 2017.2438 6727.5 2 5.205 44.7 1 K.TSAVAALTEVRAEDEAALAK.L YBR146W 2 2 20.9% 278 31925 10.3 U MRPS9 SGDID:S000000350, Chr II from 535254-536090, Verified ORF, "Mitochondrial ribosomal protein of the small subunit" * Jamie_Mitco_SPB_53.19023.19023.2 2.1795 0.2048 84.8 1914.6921 1914.3256 6184.6 9 4.507 37.5 1 F.SRLSLFRRAALAPAPMR.M * Jamie_Mitco_SPB_54.19117.19117.3 2.0378 0.2027 94.0 4943.844 4946.368 6984.5 159 4.324 12.5 1 K.LHNIDPQLT*NDEITSELSQYYKKSSMLS*NNIKIKTLDEFGR.S YNL188W 8 8 20.8% 433 50653 9.3 U KAR1 SGDID:S000005132, Chr XIV from 286309-287610, Verified ORF, "Essential protein involved in karyogamy during mating and in spindle pole body duplication during mitosis, localizes to the half-bridge of the spindle pole body, interacts with Spc72p during karyogamy, also interacts with Cdc31p" * Jamie_Mitco_SPB_31.10821.10821.2 3.9266 0.2053 100.0 1885.0122 1885.936 8902.6 1 5.614 64.3 1 K.IDEDNIENELQFT*PK.I * Jamie_Mitco_SPB_25.08928.08928.3 3.4816 0.2745 100.0 2844.1743 2846.17 6720.9 22 4.416 26.0 1 R.KQLGKPLPLPYLNS*PNSDSTPTLQR.K * Jamie_Mitco_SPB_36.12731.12731.2 3.0023 0.2809 100.0 2291.2122 2291.4795 6682.2 1 6.193 39.5 1 K.PLPLPYLNS*PNSDSTPTLQR.K * Jamie_Mitco_SPB_45.15886.15886.2 2.7003 0.2389 100.0 2372.8323 2371.4795 7970.1 1 4.126 39.5 1 K.PLPLPYLNS*PNS*DSTPTLQR.K * Jamie_Mitco_SPB_16.05457.05457.2 4.498 0.1699 100.0 1465.3722 1465.6433 7999.7 1 5.366 81.8 1 R.KEEVFTDEVLQK.K * Jamie_Mitco_SPB_37.13261.13261.2 1.9763 0.0462 91.8 2413.892 2413.754 7819.2 150 2.819 26.3 1 K.FQAQRDPLQILQSEIEMHTK.K * Jamie_Mitco_SPB_38.13365.13365.3 5.8015 0.306 100.0 2414.1243 2413.754 8933.1 1 5.762 42.1 1 K.FQAQRDPLQILQSEIEMHTK.K * Jamie_Mitco_SPB_29.10383.10383.3 3.7507 0.2755 100.0 2104.0745 2105.3904 6487.2 28 5.331 32.4 1 K.KLDTIIELLKDDTDSKEK.R YMR142C 3 3 20.1% 199 22525 11.1 U RPL13B SGDID:S000004750, Chr XIII from 551206-551203,550800-550205, reverse complement, Verified ORF, "Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl13Ap; not essential for viability; has similarity to rat L13 ribosomal protein" * Jamie_Mitco_SPB_15.05259.05259.2 3.0955 0.3107 100.0 1334.8121 1334.4313 6556.0 1 5.733 75.0 1 R.NQEIFDANVQR.L * Jamie_Mitco_SPB_37.13099.13099.2 3.2946 0.4363 100.0 2954.2322 2955.2512 9336.1 1 7.807 35.7 1 R.DGKAPEAEQVLSAAATFPIAQPATDVEAR.A * Jamie_Mitco_SPB_37.13036.13036.3 4.1837 0.1973 100.0 2957.3044 2955.2512 7966.3 1 3.918 27.7 1 R.DGKAPEAEQVLSAAATFPIAQPATDVEAR.A Reverse_YML027W 3 3 20.0% 385 42739 10.3 U YOX1 SGDID:S000004489, Chr XIII from 221406-222563, Verified ORF, "Homeodomain-containing transcriptional repressor, binds to Mcm1p and to early cell cycle boxes (ECBs) in the promoters of cell cycle-regulated genes expressed in M/G1 phase; expression is cell cycle-regulated; potential Cdc28p substrate" * Jamie_Mitco_SPB_83.29764.29764.2 1.7596 0.1144 95.3 1772.6522 1769.8722 5889.4 104 3.869 32.1 1 K.RKPS*GS*PNSVPRKAK.S * Jamie_Mitco_SPB_54.19239.19239.4 4.3987 0.2184 89.5 4536.6167 4536.1084 7871.0 338 4.095 15.8 1 N.ILKNVRSNPSTKVPTLTKKQPNLHFTLAQS*RKISPTSS*R.R * Jamie_Mitco_SPB_7.02497.02497.4 3.4837 0.2497 87.5 2400.8167 2396.7922 5618.6 16 3.955 28.0 1 K.VRSALPTAHVDLPPSPPSVTQII.T YLR075W 2 2 19.5% 221 25361 10.0 U RPL10 SGDID:S000004065, Chr XII from 282928-283593, Verified ORF, "Protein component of the large (60S) ribosomal subunit, responsible for joining the 40S and 60S subunits; regulates translation initiation; has similarity to rat L10 ribosomal protein and to members of the QM gene family" * Jamie_Mitco_SPB_57.20506.20506.3 4.4219 0.26 100.0 3359.9644 3357.7083 12833.6 1 5.267 25.9 1 K.KATVDEFPLCVHLVSNELEQLSSEALEAAR.I * Jamie_Mitco_SPB_26.09077.09077.2 3.2818 0.3699 100.0 1640.1322 1639.851 5664.4 1 6.829 62.5 1 K.WGFTNLDRPEYLK.K YLR044C 4 4 18.7% 563 61495 6.2 U PDC1 SGDID:S000004034, Chr XII from 234082-232391, reverse complement, Verified ORF, "Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde; subject to glucose-, ethanol-, and autoregulation; involved in amino acid catabolism" * Jamie_Mitco_SPB_56.19860.19860.3 4.001 0.2782 100.0 3511.3442 3511.0735 7661.3 1 4.921 27.5 1 R.LKQVNVNTVFGLPGDFNLSLLDKIYEVEGMR.W * Jamie_Mitco_SPB_59.21117.21117.3 4.3074 0.3063 100.0 3324.4443 3325.883 6402.3 6 5.409 25.9 1 K.LLQTPIDMSLKPNDAESEKEVIDTILALVK.D * Jamie_Mitco_SPB_16.05487.05487.2 2.8078 0.2621 100.0 1596.5521 1597.8516 4735.1 1 5.451 60.7 1 R.YGGVYVGTLSKPEVK.E * Jamie_Mitco_SPB_72.25872.25872.2 1.6085 0.0837 89.6 3086.0723 3085.391 10415.3 8 3.084 25.0 1 K.EAVESADLILSVGALLSDFNTGSFSYSYK.T YDR450W 3 4 18.5% 146 17038 10.3 U RPS18A SGDID:S000002858, Chr IV from 1359913-1359959,1360395-1360788, Verified ORF, "Protein component of the small (40S) ribosomal subunit; nearly identical to Rps18Bp and has similarity to E. coli S13 and rat S18 ribosomal proteins" YML026C 3 4 18.5% 146 17038 10.3 U RPS18B SGDID:S000004488, Chr XIII from 223828-223782,223380-222987, reverse complement, Verified ORF, "Protein component of the small (40S) ribosomal subunit; nearly identical to Rps18Ap and has similarity to E. coli S13 and rat S18 ribosomal proteins" Jamie_Mitco_SPB_15.05374.05374.3 4.0278 0.2722 100.0 2265.2644 2263.3826 4111.5 1 5.558 34.2 1 R.QNDITDGKDYHTLANNVESK.L Jamie_Mitco_SPB_14.04775.04775.2 2.7908 0.2662 100.0 1391.0922 1391.4802 5236.3 3 5.613 68.2 1 K.DYHTLANNVESK.L Jamie_Mitco_SPB_10.03300.03300.2 2.5365 0.1994 100.0 916.7522 917.00977 4831.2 3 4.574 91.7 2 K.LRDDLER.L YLR441C 3 3 18.0% 255 28743 10.0 U RPS1A SGDID:S000004433, Chr XII from 1018904-1018137, reverse complement, Verified ORF, "Ribosomal protein 10 (rp10) of the small (40S) subunit; nearly identical to Rps1Bp and has similarity to rat S3a ribosomal protein" Jamie_Mitco_SPB_19.06657.06657.2 2.3035 0.13 99.4 1460.3322 1459.7294 7184.4 11 4.215 53.8 1 R.NVGKTLVNKSTGLK.S Jamie_Mitco_SPB_11.03906.03906.2 2.2984 0.2645 100.0 1043.6322 1044.196 5553.7 11 4.92 68.8 1 K.LRVDEVQGK.N * Jamie_Mitco_SPB_57.20335.20335.3 3.5438 0.2069 82.8 2824.9143 2828.0713 8779.9 41 4.252 23.9 1 R.HSYAQSSHIRAIRKVIS*EILT*KE.V Reverse_YOR327C 1 1 17.4% 115 12957 8.8 U SNC2 SGDID:S000005854, Chr XV from 931076-930729, reverse complement, Verified ORF, "Vesicle membrane receptor protein (v-SNARE) involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins" * Jamie_Mitco_SPB_84.29937.29937.2 1.6997 0.114 93.4 2336.9722 2338.4502 8873.7 158 3.61 23.7 1 R.KFGQAS*IALNDAKDEIST*LR.E YKL099C 2 2 16.4% 250 29735 10.2 U UTP11 SGDID:S000001582, Chr XI from 256116-255364, reverse complement, Verified ORF, "Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA" * Jamie_Mitco_SPB_106.37816.37816.2 1.5161 0.0316 82.9 1424.6322 1421.7844 3363.9 415 3.293 45.5 1 K.KLEKGAKQLMFK.S * Jamie_Mitco_SPB_54.19246.19246.3 2.9536 0.0246 84.7 3635.3342 3637.2441 7545.2 12 2.631 22.3 1 K.FKQVKQHLQRETQLKQVQQRMDAQRELLK.K YGR254W 4 4 15.8% 437 46816 6.6 U ENO1 SGDID:S000003486, Chr VII from 1000932-1002245, Verified ORF, "Enolase I, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression is repressed in response to glucose" YHR174W 4 4 15.8% 437 46914 6.0 U ENO2 SGDID:S000001217, Chr VIII from 451327-452640, Verified ORF, "Enolase II, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression is induced in response to glucose" Jamie_Mitco_SPB_71.25339.25339.2 3.1046 0.4604 100.0 3258.9521 3259.551 5134.1 1 6.981 28.1 1 R.YGASAGNVGDEGGVAPNIQTAEEALDLIVDAIK.A Jamie_Mitco_SPB_26.09358.09358.2 2.1062 0.0599 96.6 1376.3121 1374.4993 7249.9 2 3.139 54.5 1 K.IGLDCASSEFFK.D Jamie_Mitco_SPB_53.19078.19078.3 3.5084 0.3148 100.0 2983.9744 2986.269 8142.7 176 5.898 22.8 1 K.RYPIVSIEDPFAEDDWEAWSHFFK.T Jamie_Mitco_SPB_53.18940.18940.3 3.165 0.0368 90.2 2991.1443 2990.0815 8137.9 3 2.947 27.3 1 R.Y*PIVS*IEDPFAEDDWEAWSHFFK.T YBR181C 2 2 15.7% 236 26996 10.4 U RPS6B SGDID:S000000385, Chr II from 592769-592764,592411-591707, reverse complement, Verified ORF, "Protein component of the small (40S) ribosomal subunit; identical to Rps6Ap and has similarity to rat S6 ribosomal protein" YPL090C 2 2 15.7% 236 26996 10.4 U RPS6A SGDID:S000006011, Chr XVI from 378392-378387,377992-377288, reverse complement, Verified ORF, "Protein component of the small (40S) ribosomal subunit; identical to Rps6Bp and has similarity to rat S6 ribosomal protein" Jamie_Mitco_SPB_28.10035.10035.3 2.9298 0.0847 93.0 2188.9143 2188.3984 7256.5 7 3.727 32.9 1 R.IGQEVDGEAVGDEFKGYVFK.I Jamie_Mitco_SPB_15.05227.05227.2 2.7504 0.3913 100.0 1900.2922 1902.1118 5633.4 1 6.027 43.8 1 K.KGEQELEGLTDTTVPKR.L contaminant_KERATIN21 6 6 15.4% 357 39219 5.2 U no description Jamie_Mitco_SPB_11.03904.03904.2 2.7247 0.1669 95.5 1195.0122 1195.3176 4778.7 1 5.748 93.8 1 N.KYEDEINKR.T Jamie_Mitco_SPB_9.03005.03005.2 2.13 0.144 99.8 1067.3722 1067.1436 6192.7 305 4.211 64.3 1 K.YEDEINKR.T Jamie_Mitco_SPB_40.14340.14340.1 2.6538 0.1875 100.0 1330.02 1330.5211 5956.2 2 6.415 59.1 1 R.NLDLDSIIAEVK.A Jamie_Mitco_SPB_60.21303.21303.2 5.3215 0.5087 100.0 2419.632 2419.694 8311.9 1 8.061 60.0 1 R.NLDLDSIIAEVKAQYEEIAQR.S Jamie_Mitco_SPB_18.06285.06285.2 3.0677 0.3224 100.0 1359.3722 1358.5345 5938.9 1 5.148 77.3 1 K.NKLEGLEDALQK.A Jamie_Mitco_SPB_30.10584.10584.1 1.4818 0.3292 100.0 1320.77 1320.396 4673.0 1 4.986 45.8 1 G.GSSTIKYTTTSSS.S YPR004C 1 1 14.8% 344 36803 8.5 U YPR004C SGDID:S000006208, Chr XVI from 565038-564004, reverse complement, Verified ORF, "Electron transfer flavoprotein complex subunit ETF-alpha; contains a FAD binding domain; interacts with YFH1, the yeast frataxin homolog" * Jamie_Mitco_SPB_46.16354.16354.4 2.8787 0.1181 84.9 5480.2563 5482.125 10767.5 294 3.678 12.7 1 R.SSLSLLAAAQKLSNPIT*AVITGSKAEKTAEALKSSYSCSNLEKLVIFEDSK.L YNL126W 6 7 14.4% 846 98227 7.5 U SPC98 SGDID:S000005070, Chr XIV from 387229-389769, Verified ORF, "Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque" * Jamie_Mitco_SPB_102.36545.36545.2 1.9849 0.2309 85.1 2395.5522 2395.7642 5689.6 3 3.865 32.5 1 A.PQLLSQSHLQTFVSDVVNLLR.S * Jamie_Mitco_SPB_26.09131.09131.2 2.045 0.0024 88.9 1857.0122 1857.1136 7639.7 2 3.644 37.5 1 R.SSTKSATQLGPLIDFYK.L * Jamie_Mitco_SPB_23.08157.08157.3 4.213 0.444 100.0 2218.6743 2219.4802 5747.7 2 6.861 40.8 1 K.SSGLNMENLANHEHLLSPVR.A * Jamie_Mitco_SPB_25.08877.08877.2 3.347 0.3865 100.0 1812.3522 1811.8744 4958.6 1 7.046 57.1 1 R.APSIYTEAS*FENMDR.F * Jamie_Mitco_SPB_51.18215.18215.3 4.135 0.2742 100.0 3885.6843 3885.1047 9978.6 1 6.365 25.8 2 R.ATYGEFFIAENTDTNGTDDDFIYHIPIEFNQER.V * Jamie_Mitco_SPB_25.08839.08839.2 3.186 0.2311 100.0 1808.5322 1807.9952 5326.7 1 5.187 56.7 1 R.ADLKNDGDEELFLLSK.S YAL005C 8 8 14.2% 642 69768 5.1 U SSA1 SGDID:S000000004, Chr I from 141433-139505, reverse complement, Verified ORF, "ATPase involved in protein folding and nuclear localization signal (NLS)-directed nuclear transport; member of heat shock protein 70 (HSP70) family; forms a chaperone complex with Ydj1p; localized to the nucleus, cytoplasm, and cell wall" Jamie_Mitco_SPB_14.04699.04699.2 4.1855 0.3804 100.0 1677.9122 1676.6964 7247.5 1 7.338 73.3 1 K.ATAGDTHLGGEDFDNR.L * Jamie_Mitco_SPB_21.07285.07285.2 2.223 0.1853 99.8 1817.5521 1817.0496 6833.1 1 4.044 46.9 1 K.LDKSQVDEIVLVGGSTR.I Jamie_Mitco_SPB_8.02583.02583.3 2.8517 0.2009 100.0 1348.7644 1347.5143 5419.4 1 4.718 50.0 1 K.SNKITITNDKGR.L Jamie_Mitco_SPB_8.02673.02673.2 1.9356 0.1341 98.9 1019.3722 1018.1582 3809.6 81 3.948 68.8 1 K.ITITNDKGR.L * Jamie_Mitco_SPB_9.03191.03191.2 2.2829 0.0466 98.7 962.03217 962.088 3670.4 159 3.546 78.6 1 R.LSKEDIEK.M * Jamie_Mitco_SPB_32.11415.11415.3 3.7222 0.2047 100.0 3128.4844 3127.4475 5160.6 1 4.799 25.0 1 R.LSKEDIEKMVAEAEKFKEEDEKESQR.I * Jamie_Mitco_SPB_32.11512.11512.4 3.5321 0.0739 83.1 3128.6565 3127.4475 8571.5 2 3.796 25.3 1 R.LSKEDIEKMVAEAEKFKEEDEKESQR.I * Jamie_Mitco_SPB_16.05693.05693.3 3.6953 0.292 100.0 2166.1743 2164.3313 4524.9 1 5.226 32.9 1 K.NTISEAGDKLEQADKDTVTK.K YGL008C 9 10 13.6% 918 99619 5.1 U PMA1 SGDID:S000002976, Chr VII from 482671-479915, reverse complement, Verified ORF, "Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plasma membrane potential; part of the P2 subgroup of cation-transporting ATPases" * Jamie_Mitco_SPB_51.18174.18174.2 4.4664 0.439 100.0 3262.7122 3261.6104 4313.0 1 7.925 35.0 1 R.DGQLVEIPANEVVPGDILQLEDGTVIPTDGR.I * Jamie_Mitco_SPB_13.04447.04447.2 2.9765 0.2274 100.0 1274.1322 1273.3855 6823.8 7 5.094 63.6 1 K.VTAVVESPEGER.I * Jamie_Mitco_SPB_34.12081.12081.4 5.2655 0.4198 100.0 3845.9365 3848.261 6807.9 1 6.745 22.2 1 K.GAPLFVLKTVEEDHPIPEDVHENYENKVAELASR.G * Jamie_Mitco_SPB_13.04653.04653.3 3.2543 0.1073 95.4 2296.2844 2295.3806 8090.4 1 4.056 34.7 1 K.TVEEDHPIPEDVHENYENK.V * Jamie_Mitco_SPB_21.07223.07223.2 1.8229 0.2608 85.7 2777.112 2778.9458 6914.2 108 6.002 26.1 1 K.TVEEDHPIPEDVHENYENKVAELA.S * Jamie_Mitco_SPB_20.07186.07186.3 5.8784 0.5725 100.0 3020.1843 3022.2114 6725.3 1 9.144 40.0 1 K.TVEEDHPIPEDVHENYENKVAELASR.G * Jamie_Mitco_SPB_21.07229.07229.4 4.33 0.4524 100.0 3020.2566 3022.2114 7383.2 1 6.765 28.0 2 K.TVEEDHPIPEDVHENYENKVAELASR.G * Jamie_Mitco_SPB_54.19427.19427.3 5.8783 0.4904 100.0 3262.6743 3263.5623 6526.1 1 8.419 31.5 1 R.LGLGGGGDMPGSELADFVENADGFAEVFPQHK.Y * Jamie_Mitco_SPB_13.04528.04528.2 2.2325 7.0E-4 90.8 1548.8522 1546.6775 6650.0 2 3.211 53.3 1 K.KADTGIAVEGATDAAR.S YDR418W 1 1 13.3% 165 17823 9.4 U RPL12B SGDID:S000002826, Chr IV from 1301606-1302103, Verified ORF, "Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl12Ap; rpl12a rpl12b double mutant exhibits slow growth and slow translation; has similarity to E. coli L11 and rat L12 ribosomal proteins" YEL054C 1 1 13.3% 165 17823 9.4 U RPL12A SGDID:S000000780, Chr V from 53218-52721, reverse complement, Verified ORF, "Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl12Bp; rpl12a rpl12b double mutant exhibits slow growth and slow translation; has similarity to E. coli L11 and rat L12 ribosomal proteins" Jamie_Mitco_SPB_37.13137.13137.2 2.242 0.2375 91.2 2464.0723 2465.6787 7966.5 1 4.667 38.1 1 V.DFKNPHDIIEGINAGEIEIPEN.- YBR085W 2 2 13.0% 307 33313 9.8 U AAC3 SGDID:S000000289, Chr II from 415977-416900, Verified ORF, "Mitochondrial inner membrane ADP/ATP translocator, exchanges cytosolic ADP for mitochondrially synthesized ATP; expressed under anaerobic conditions; similar to Pet9p and Aac1p; has roles in maintenance of viability and in respiration" * Jamie_Mitco_SPB_48.17155.17155.2 1.5235 0.067 86.5 2991.392 2992.3218 9058.7 53 3.21 21.2 1 R.TRLAADAKSS*KKGGARQFNGLTDVYKK.T * Jamie_Mitco_SPB_79.28379.28379.2 1.6518 0.0954 91.7 1496.1122 1495.5864 4102.5 74 3.053 37.5 1 K.KIVAS*EGVGS*LFK.G YGR097W 9 9 12.6% 1146 126864 9.3 U ASK10 SGDID:S000003329, Chr VII from 678699-682139, Verified ORF, "Component of the RNA polymerase II holoenzyme, phosphorylated in response to oxidative stress; has a role in destruction of Ssn8p, which relieves repression of stress-response genes" * Jamie_Mitco_SPB_21.07439.07439.2 4.0078 0.0805 100.0 2202.2922 2202.2097 8305.6 1 6.664 52.5 1 K.SSAYS*SSVSIADTYANANNAK.A * Jamie_Mitco_SPB_99.35302.35302.2 1.6288 0.0171 83.0 1978.3522 1975.975 5733.9 21 3.17 35.3 1 R.KSSSST*Y*LSGSSQASLPK.Y * Jamie_Mitco_SPB_34.12037.12037.3 3.1373 0.0485 90.3 3368.3044 3370.4448 7069.7 90 3.531 19.8 1 R.NSVNIGSHTPCLTDS*T*FTLQDGTTTSVNLK.G * Jamie_Mitco_SPB_27.09454.09454.3 2.8751 0.0944 93.1 2717.1543 2715.8552 4731.6 9 2.963 25.0 1 R.AEKPQYIHIQNNS*LADFDGNGFR.S * Jamie_Mitco_SPB_29.10138.10138.2 4.0697 0.4071 100.0 1890.7922 1891.088 8568.7 1 8.395 68.8 1 K.VNTPAIDDYGNLITVER.R * Jamie_Mitco_SPB_36.12671.12671.2 1.6842 0.0281 85.5 2128.0122 2127.2754 8043.1 184 2.887 26.5 1 K.VNTPAIDDYGNLIT*VERR.P * Jamie_Mitco_SPB_15.05055.05055.2 1.8923 0.0367 89.5 1615.1122 1614.796 5175.5 151 2.769 34.4 1 R.STSAGNSITANAPVVPK.V * Jamie_Mitco_SPB_17.05829.05829.2 2.6035 0.1752 100.0 1694.9922 1694.796 7004.5 129 3.725 43.8 1 R.STS*AGNSITANAPVVPK.V * Jamie_Mitco_SPB_12.04193.04193.2 3.7415 0.3879 100.0 1777.0922 1776.7281 4171.3 1 7.693 59.4 1 R.GNNS*AQNLTTSSSTASR.V YOR272W 2 2 12.6% 460 51359 7.0 U YTM1 SGDID:S000005798, Chr XV from 832810-834192, Verified ORF, "Constituent of 66S pre-ribosomal particles, required for maturation of the large ribosomal subunit" * Jamie_Mitco_SPB_14.04951.04951.2 3.6257 0.3323 100.0 2377.632 2378.379 6510.8 1 7.77 47.5 1 K.LTSQQQAQEDDDDEVNIEDGK.T * Jamie_Mitco_SPB_120.43066.43066.4 3.685 0.2553 94.4 4195.4966 4190.668 9202.2 36 4.471 17.1 1 R.ILSASYDNSIGFWSTIYKEMTVVDPLEDINNPNNKIS.T contaminant_KERATIN18 6 6 12.5% 562 59822 8.0 U no description * Jamie_Mitco_SPB_4.01389.01389.2 2.6253 0.2525 100.0 940.47217 939.01886 3542.3 2 5.407 75.0 1 R.RGFSASSAR.L Jamie_Mitco_SPB_20.07182.07182.2 2.7303 0.4955 100.0 1759.8922 1761.0282 7181.7 3 7.043 46.4 1 K.VLDTKWTLLQEQGTK.T Jamie_Mitco_SPB_40.14340.14340.1 2.6538 0.1875 100.0 1330.02 1330.5211 5956.2 2 6.415 59.1 1 R.NLDLDSIIAEVK.A Jamie_Mitco_SPB_60.21303.21303.2 5.3215 0.5087 100.0 2419.632 2419.694 8311.9 1 8.061 60.0 1 R.NLDLDSIIAEVKAQYEEIAQR.S Jamie_Mitco_SPB_18.06285.06285.2 3.0677 0.3224 100.0 1359.3722 1358.5345 5938.9 1 5.148 77.3 1 K.NKLEGLEDALQK.A Jamie_Mitco_SPB_30.10584.10584.1 1.4818 0.3292 100.0 1320.77 1320.396 4673.0 1 4.986 45.8 1 G.GSSTIKYTTTSSS.S YLR340W 2 3 12.5% 312 33717 4.8 U RPP0 SGDID:S000004332, Chr XII from 805887-806825, Verified ORF, "Conserved ribosomal protein P0 similar to rat P0, human P0, and E. coli L10e; shown to be phosphorylated on serine 302" * Jamie_Mitco_SPB_57.20423.20423.2 2.1678 0.0096 90.8 1967.3522 1966.3275 8798.3 60 2.879 34.4 1 R.GFLSDLPDFEKLLPFVK.G * Jamie_Mitco_SPB_55.19663.19663.2 5.4308 0.465 100.0 2474.9521 2474.773 9614.0 1 8.592 52.4 2 K.DLLAVAIAASYHYPEIEDLVDR.I Reverse_YOR204W 3 3 12.1% 604 65553 7.8 U DED1 SGDID:S000005730, Chr XV from 722911-724725, Verified ORF, "ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility" * Jamie_Mitco_SPB_71.25549.25549.4 3.424 0.1248 88.4 3414.3765 3417.4346 5338.5 16 3.155 20.6 1 R.S*SGWGGASAGGAKRYDRNNNRGFGGRRSNS*R.S * Jamie_Mitco_SPB_17.06040.06040.2 2.0981 0.0606 95.2 2082.0723 2082.3677 6638.8 72 3.77 30.0 1 R.AAVATAVLLT*AAGSRFAALAR.E * Jamie_Mitco_SPB_49.17521.17521.2 1.8054 0.0687 90.7 2152.872 2150.0183 7469.1 148 3.049 27.5 1 R.GNS*RS*GGNNGGFFGGNGYGGR.R YAL038W 4 4 12.0% 500 54545 7.7 U CDC19 SGDID:S000000036, Chr I from 71787-73289, Verified ORF, "Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration" * Jamie_Mitco_SPB_23.08206.08206.3 2.6815 0.1734 96.5 2186.5745 2186.4705 4409.8 22 3.78 31.6 1 R.TANDVLTIREVLGEQGKDVK.I * Jamie_Mitco_SPB_43.15252.15252.3 4.3759 0.4514 100.0 2571.5044 2570.8174 7879.6 1 7.257 35.7 1 R.GVFPFVFEKEPVSDWTDDVEAR.I * Jamie_Mitco_SPB_15.05401.05401.1 1.6211 0.0309 95.3 821.01 820.964 7094.4 7 4.073 75.0 1 R.INFGIEK.A * Jamie_Mitco_SPB_40.14151.14151.2 1.4813 0.052 85.1 1217.8322 1215.3483 5139.4 73 2.834 45.0 1 K.GDTYVSIQGFK.A YOR019W 3 3 11.6% 730 83366 9.7 U YOR019W SGDID:S000005545, Chr XV from 368126-370318, Uncharacterized ORF, "Hypothetical protein" * Jamie_Mitco_SPB_73.26118.26118.2 1.7682 0.0375 88.1 2524.152 2522.9194 4611.5 51 2.801 28.6 1 K.ILQQKVGYPNNPKTIVCHINGK.K * Jamie_Mitco_SPB_71.25371.25371.2 1.8217 0.0097 85.7 2468.0122 2468.79 10041.4 8 2.803 30.0 1 K.MISNRKKTAKDDTEWAPGYQK.E * Jamie_Mitco_SPB_59.21138.21138.4 3.7273 0.1327 94.7 4845.8965 4848.2515 6297.7 74 2.894 15.0 1 K.FRKSLIPYSS*SEEQNTTTTIKLS*SSPTSQIKFATSVKHKDGR.A YMR186W 4 4 11.5% 705 80900 4.8 U HSC82 SGDID:S000004798, Chr XIII from 632354-634471, Verified ORF, "Cytoplasmic chaperone of the Hsp90 family, redundant in function and nearly identical with Hsp82p, and together they are essential; expressed constitutively at 10-fold higher basal levels that HSP82 and induced 2-3 fold by heat shock" * Jamie_Mitco_SPB_34.11919.11919.2 3.9421 0.4417 100.0 1746.6721 1746.0593 6751.9 1 8.418 60.7 1 R.HSEFVAYPIQLLVTK.E * Jamie_Mitco_SPB_23.07937.07937.2 2.3927 0.1779 99.8 1820.4922 1819.922 8161.5 1 3.954 53.6 1 R.NPSDITQEEYNAFYK.S * Jamie_Mitco_SPB_84.30210.30210.3 3.4313 0.0542 93.1 3748.7644 3748.2214 9122.3 2 3.465 21.1 1 R.VFITDEAEDLIPEWLSFVKGVVDSEDLPLNLSR.E * Jamie_Mitco_SPB_34.12101.12101.2 3.0788 0.2865 100.0 2127.5322 2127.3123 7641.3 3 5.998 41.2 1 K.KLIEAFNEIAEDSEQFDK.F YHR203C 2 2 11.5% 261 29410 10.1 U RPS4B SGDID:S000001246, Chr VIII from 505530-505517,505247-504476, reverse complement, Verified ORF, "Protein component of the small (40S) ribosomal subunit; identical to Rps4Bp and has similarity to rat S4 ribosomal protein" YJR145C 2 2 11.5% 261 29410 10.1 U RPS4A SGDID:S000003906, Chr X from 702983-702970,702713-701942, reverse complement, Verified ORF, "Protein component of the small (40S) ribosomal subunit; mutation affects 20S pre-rRNA processing; identical to Rps4Bp and has similarity to rat S4 ribosomal protein" Jamie_Mitco_SPB_18.06133.06133.3 2.6579 0.1903 99.5 1236.8944 1238.3885 4939.8 15 4.377 45.0 1 R.HDGGFDLVHIK.D Jamie_Mitco_SPB_30.10660.10660.3 2.9523 0.1467 96.5 2119.4944 2117.496 6262.4 96 3.316 26.4 1 R.LNNVFVIGEQGKPYISLPK.G YDR212W 4 4 11.3% 559 60481 6.5 U TCP1 SGDID:S000002620, Chr IV from 887227-888906, Verified ORF, "Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in maintenance of actin cytoskeleton; homolog to Drosophila melanogaster and mouse tailless complex polypeptide" * Jamie_Mitco_SPB_23.07956.07956.2 1.9963 0.1348 98.4 1824.4122 1823.9977 6682.9 8 3.823 37.5 1 R.SDTLFLGGEKISGDDIR.N * Jamie_Mitco_SPB_52.18443.18443.2 1.9339 0.0333 89.9 2005.5922 2006.1284 7399.5 142 2.976 32.4 1 R.EIGDGTT*S*VVIIASELLK.R * Jamie_Mitco_SPB_16.05746.05746.2 2.3644 0.0999 99.0 1402.5521 1400.4596 7530.9 1 3.747 68.2 1 R.GANDYSLDEMER.S * Jamie_Mitco_SPB_15.05232.05232.2 2.7349 0.1789 100.0 1619.1322 1617.84 7687.7 2 4.996 50.0 1 K.TLAVNAAKDSSELVAK.L YBR048W 1 1 10.9% 156 17749 10.8 U RPS11B SGDID:S000000252, Chr II from 332829-332873,333385-333810, Verified ORF, "Protein component of the small (40S) ribosomal subunit; identical to Rps11Ap and has similarity to E. coli S17 and rat S11 ribosomal proteins" YDR025W 1 1 10.9% 156 17749 10.8 U RPS11A SGDID:S000002432, Chr IV from 491511-491555,491895-492320, Verified ORF, "Protein component of the small (40S) ribosomal subunit; identical to Rps11Bp and has similarity to E. coli S17 and rat S11 ribosomal proteins" Jamie_Mitco_SPB_17.06025.06025.2 2.8173 0.272 100.0 1857.6322 1857.1266 6546.3 77 4.847 37.5 1 R.VQVGDIVTVGQCRPISK.T YMR001C 5 5 10.8% 705 81031 9.0 U CDC5 SGDID:S000004603, Chr XIII from 271136-269019, reverse complement, Verified ORF, "Polo-like kinase with similarity to Xenopus Plx1 and S. pombe Plo1p; found at bud neck, nucleus and SPBs; has multiple functions in mitosis and cytokinesis through phosphorylation of substrates; may be a Cdc28p substrate" * Jamie_Mitco_SPB_14.04785.04785.2 2.7158 0.3155 100.0 1743.2722 1743.9578 5053.1 69 5.462 43.3 1 K.LVHTPIKGNT*ADLVGK.E * Jamie_Mitco_SPB_32.11367.11367.3 2.7238 0.076 91.4 2235.4443 2236.511 6045.2 140 3.623 27.8 1 R.KYT*ICGTPNYIAPEVLMGK.H * Jamie_Mitco_SPB_33.11589.11589.2 2.6465 0.0839 99.0 2237.412 2236.511 8315.5 26 3.689 36.1 1 R.KYT*ICGTPNYIAPEVLMGK.H * Jamie_Mitco_SPB_75.26953.26953.3 2.6988 0.1156 93.0 3306.2944 3305.903 4039.0 67 3.741 21.2 1 K.ILIRDILSLDPIERPSLTEIMDYVWFR.G * Jamie_Mitco_SPB_19.06595.06595.2 3.644 0.4469 100.0 1607.8121 1607.8627 8248.7 1 7.536 73.1 1 K.SLLLESMSSDKIQR.Q YDR172W 4 4 10.8% 685 76551 7.0 U SUP35 SGDID:S000002579, Chr IV from 808319-810376, Verified ORF, "Translation termination factor eRF3; altered protein conformation creates the [PSI(+)] prion, a dominant cytoplasmically inherited protein aggregate that alters translational fidelity and creates a nonsense suppressor phenotype" * Jamie_Mitco_SPB_31.10953.10953.2 4.5803 0.5315 100.0 2069.4521 2070.1458 9209.9 1 9.429 61.8 1 K.EQEEEVDDEVVNDMFGGK.D * Jamie_Mitco_SPB_24.08521.08521.3 2.5588 0.1324 93.9 1815.7743 1813.079 4725.6 7 3.728 39.1 1 K.STMGGNLLYLTGSVDKR.T * Jamie_Mitco_SPB_25.08728.08728.2 2.6584 0.2125 100.0 1423.9321 1423.7594 6997.9 2 5.96 62.5 1 R.HINAPFMLPIAAK.M * Jamie_Mitco_SPB_48.16999.16999.2 4.3409 0.4979 100.0 3017.0122 3017.2725 9603.0 1 8.579 38.0 1 K.TAVEIQNIYNETENEVDMAMCGEQVK.L YFR028C 3 3 10.5% 551 61907 8.0 U CDC14 SGDID:S000001924, Chr VI from 210056-208401, reverse complement, Verified ORF, "Protein phosphatase required for mitotic exit; located in the nucleolus until liberated by the FEAR and Mitotic Exit Network in anaphase, enabling it to act on key substrates to effect a decrease in CDK/B-cyclin activity and mitotic exit" * Jamie_Mitco_SPB_18.06193.06193.2 2.2445 0.1734 99.8 1806.7122 1805.917 6336.2 1 3.997 50.0 1 R.DLTMTPPS*NGHGALSAR.N * Jamie_Mitco_SPB_121.43511.43511.3 3.4563 0.1896 99.5 2991.2043 2991.04 6029.6 307 4.474 21.0 1 R.KVVIESNNSDDES*MQDTNGTSNHYPK.V * Jamie_Mitco_SPB_83.29770.29770.2 1.6526 0.0013 82.3 1780.1122 1782.7788 6396.9 177 3.103 35.7 1 K.VSRKKNDIS*SAS*SSR.M Reverse_YGR192C 2 2 10.5% 332 35747 7.0 U TDH3 SGDID:S000003424, Chr VII from 883815-882817, reverse complement, Verified ORF, "Glyceraldehyde-3-phosphate dehydrogenase, isozyme 3, involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell-wall " * Jamie_Mitco_SPB_19.06486.06486.3 2.8371 0.1951 99.0 2224.7944 2222.5872 6332.7 379 3.631 27.6 1 K.LKGEAAAKVVKKIEDYTTEK.N * Jamie_Mitco_SPB_52.18516.18516.2 1.7916 0.034 88.0 1564.5122 1563.5352 4417.5 120 2.947 39.3 1 K.AAGTSS*PIINGSAT*R.G YOR315W 2 2 10.4% 346 39021 8.1 U SFG1 SGDID:S000005842, Chr XV from 904755-905795, Verified ORF, "Nuclear protein, putative transcription factor required for growth of superficial pseudohyphae (which do not invade the agar substrate) but not for invasive pseudohyphal growth; may act together with Phd1p; potential Cdc28p substrate" * Jamie_Mitco_SPB_75.26879.26879.2 1.6612 0.0837 90.5 1530.8922 1528.8419 4651.0 34 3.453 38.5 1 K.IGLVIPSTPSKKCK.Y * Jamie_Mitco_SPB_60.21251.21251.3 3.0989 0.2274 100.0 2932.4343 2935.189 5128.9 2 4.584 26.1 1 K.CKYSSGFIAEDTTPSKRFRLY*QAK.F Reverse_YPR143W 2 2 10.4% 250 28212 5.7 U RRP15 SGDID:S000006347, Chr XVI from 818319-819071, Verified ORF, "Nucleolar protein, constituent of pre-60S ribosomal particles; required for processing of the 27S pre-rRNA at the A2 site to yield 5.8S and 25S rRNA" * Jamie_Mitco_SPB_24.08361.08361.2 1.5351 0.0121 80.8 2214.5923 2215.333 7597.4 103 3.501 32.4 1 K.EEKNKIESLNES*VEKETK.V * Jamie_Mitco_SPB_10.03531.03531.1 1.2895 0.1328 97.1 885.62 886.1691 4023.0 11 3.459 57.1 1 R.AKGLLKKK.E YLR303W 2 2 10.1% 444 48672 6.4 U MET17 SGDID:S000004294, Chr XII from 732544-733878, Verified ORF, "O-acetyl homoserine-O-acetyl serine sulfhydrylase, required for sulfur amino acid synthesis" * Jamie_Mitco_SPB_29.10101.10101.3 2.2726 0.1109 90.5 2087.3943 2087.2065 6227.9 22 3.661 34.4 1 R.FVEGDNPEEFEKVFDER.T * Jamie_Mitco_SPB_104.37212.37212.2 1.8478 0.1185 96.7 3104.132 3102.4675 9678.5 45 3.825 22.2 1 R.VSVGIEFIDDIIADFQQSFETVFAGQKP.- YGR215W 1 1 10.0% 110 12393 10.2 U RSM27 SGDID:S000003447, Chr VII from 922180-922512, Verified ORF, "Mitochondrial ribosomal protein of the small subunit" * Jamie_Mitco_SPB_6.02119.02119.2 1.7595 0.0802 93.0 1018.77216 1018.1582 6585.7 5 4.527 70.0 1 K.DAAATAKGKGK.K YGR159C 3 3 9.9% 414 44535 4.9 U NSR1 SGDID:S000003391, Chr VII from 807661-806417, reverse complement, Verified ORF, "Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ribosome biogenesis" * Jamie_Mitco_SPB_16.05716.05716.3 2.9767 0.1042 94.1 1849.3744 1849.0073 6260.5 11 4.088 36.7 1 K.QKNEETEEPATIFVGR.L * Jamie_Mitco_SPB_5.01500.01500.1 1.6003 0.0238 95.8 903.96 905.05695 5399.7 252 2.909 57.1 1 K.AIQEMQGK.E * Jamie_Mitco_SPB_66.23487.23487.2 1.4723 0.0081 80.3 1684.7322 1682.75 5872.5 95 2.724 31.2 1 R.GGFRPSGS*GANTAPLGR.S YHR090C 1 1 9.9% 282 32086 8.0 U YNG2 SGDID:S000001132, Chr VIII from 284627-283779, reverse complement, Verified ORF, "Subunit of the NuA4 histone acetyltransferase complex that acetylates histone H4 and H2A; has similarity to the human tumor suppressor ING1" * Jamie_Mitco_SPB_37.13144.13144.2 2.5085 0.1002 99.0 2918.912 2918.1128 6610.0 2 3.45 27.8 1 K.NIALLEEDGVLAPVEEDGDMDSAAEASR.E YER115C 1 1 9.9% 191 21512 9.5 U SPR6 SGDID:S000000917, Chr V from 394863-394288, reverse complement, Verified ORF, "Protein of unknown function, expressed during sporulation; not required for sporulation, but gene exhibits genetic interactions with other genes required for sporulation" * Jamie_Mitco_SPB_79.28247.28247.2 2.2852 0.2829 95.1 2077.412 2080.191 5919.4 1 4.553 41.7 1 R.FSCDQSAVLGCAASSTRVY.D YLR167W 2 3 9.9% 152 17216 9.9 U RPS31 SGDID:S000004157, Chr XII from 498949-499407, Verified ORF, "Fusion protein that is cleaved to yield a ribosomal protein of the small (40S) subunit and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B" * Jamie_Mitco_SPB_12.04164.04164.2 3.0442 0.2933 100.0 1739.1122 1739.926 6525.7 1 6.067 57.1 1 K.SKIQDKEGIPPDQQR.L * Jamie_Mitco_SPB_10.03565.03565.2 2.7065 0.2262 100.0 1525.1522 1524.6738 5013.1 1 5.044 70.8 2 K.IQDKEGIPPDQQR.L contaminant_KERATIN17 6 6 9.8% 590 62461 8.1 U no description Jamie_Mitco_SPB_20.07182.07182.2 2.7303 0.4955 100.0 1759.8922 1761.0282 7181.7 3 7.043 46.4 1 K.VLDTKWTLLQEQGTK.T Jamie_Mitco_SPB_11.03904.03904.2 2.7247 0.1669 95.5 1195.0122 1195.3176 4778.7 1 5.748 93.8 1 N.KYEDEINKR.T Jamie_Mitco_SPB_9.03005.03005.2 2.13 0.144 99.8 1067.3722 1067.1436 6192.7 305 4.211 64.3 1 K.YEDEINKR.T Jamie_Mitco_SPB_40.14340.14340.1 2.6538 0.1875 100.0 1330.02 1330.5211 5956.2 2 6.415 59.1 1 R.NLDLDSIIAEVK.A * Jamie_Mitco_SPB_23.08203.08203.3 5.3013 0.4066 100.0 2664.9543 2662.832 9806.5 1 7.3 38.1 1 R.SRTEAESWYQTKYEELQQTAGR.H * Jamie_Mitco_SPB_14.04989.04989.2 2.0083 0.2562 100.0 1243.2722 1243.3153 6466.4 1 4.814 77.8 1 R.TEAESWYQTK.Y YBR118W 3 3 9.8% 458 50033 9.0 U TEF2 SGDID:S000000322, Chr II from 477665-479041, Verified ORF, "Translational elongation factor EF-1 alpha; also encoded by TEF1; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA) to ribosomes" YPR080W 3 3 9.8% 458 50033 9.0 U TEF1 SGDID:S000006284, Chr XVI from 700592-701968, Verified ORF, "Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA) to ribosomes" Jamie_Mitco_SPB_121.43335.43335.3 2.2605 0.1513 94.0 1560.6543 1561.7812 6224.9 5 4.171 33.9 1 K.SHINVVVIGHVDSGK.S Jamie_Mitco_SPB_9.03216.03216.1 1.2277 0.0781 95.7 895.01 895.0434 4873.4 2 3.428 66.7 1 R.TIEKFEK.E Jamie_Mitco_SPB_18.06210.06210.2 3.6864 0.4551 100.0 2550.412 2551.793 5661.5 1 7.356 43.2 1 K.SVEMHHEQLEQGVPGDNVGFNVK.N YMR055C 2 2 9.8% 306 35028 8.6 U BUB2 SGDID:S000004659, Chr XIII from 387020-386100, reverse complement, Verified ORF, "Mitotic exit network regulator, forms GTPase-activating Bfa1p-Bub2p complex that binds Tem1p and spindle pole bodies, blocks cell cycle progression before anaphase in response to spindle and kinetochore damage" * Jamie_Mitco_SPB_24.08475.08475.2 4.536 0.4148 100.0 2117.1921 2118.3948 4834.3 1 7.851 61.8 1 R.YLILSEGLPISEDKQQQR.T * Jamie_Mitco_SPB_33.11689.11689.2 2.0237 0.0506 94.2 1468.6921 1466.5902 4159.7 114 3.334 45.5 1 R.QFPDFDADEIIR.L YOR078W 1 1 9.8% 214 24385 6.6 U BUD21 SGDID:S000005604, Chr XV from 472726-473370, Verified ORF, "Component of small ribosomal subunit (SSU) processosome that contains U3 snoRNA; originally isolated as bud-site selection mutant that displays a random budding pattern" * Jamie_Mitco_SPB_52.18400.18400.2 2.8328 0.0874 99.8 2358.2122 2358.6018 9599.5 10 4.465 32.5 1 K.SVNETEVTDEVIAELPEELLK.N Reverse_YER087W 2 2 9.7% 576 65880 7.4 U YER087W SGDID:S000000889, Chr V from 330572-332302, Uncharacterized ORF, "Hypothetical protein" * Jamie_Mitco_SPB_17.06010.06010.2 2.0537 0.0573 93.5 1951.0922 1952.3752 4960.7 187 3.292 32.4 1 K.SGVIVCLPIGMAHSLKIR.A * Jamie_Mitco_SPB_18.06425.06425.4 3.0779 0.2429 98.8 4635.2163 4639.065 7061.9 325 5.318 15.8 1 R.RVFGVT*QLLEQTTESKLT*NNSLSKPHFIHCSRSLTRYK.L YIL150C 1 1 9.6% 571 65815 9.7 U MCM10 SGDID:S000001412, Chr IX from 62728-61013, reverse complement, Verified ORF, "Essential, chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins" * Jamie_Mitco_SPB_63.22522.22522.4 3.013 0.1143 84.6 6198.9365 6196.866 9153.1 19 3.262 13.0 1 R.GTS*AKRIELNGGY*ALGAPTKVDSQPSLYKAKGENGFNIIKGTRKRLSEEEERLKK.S YIL022W 1 1 9.5% 431 48854 9.4 U TIM44 SGDID:S000001284, Chr IX from 311163-312458, Verified ORF, "Peripheral mitochondrial membrane protein involved in mitochondrial protein import, tethers essential chaperone Ssc1p to the translocon channel at the matrix side of the inner membrane" * Jamie_Mitco_SPB_106.37830.37830.4 3.0176 0.1349 86.5 4939.2563 4944.3403 10763.5 28 3.584 16.2 1 K.KEWEKS*QELQENIKTLQDASGKLGES*EAYKKAREAYLKAQR.G YNR049C 1 1 9.5% 210 23351 10.0 U MSO1 SGDID:S000005332, Chr XIV from 713657-713025, reverse complement, Verified ORF, "Probable component of the secretory vesicle docking complex, acts at a late step in secretion; shows genetic and physical interactions with Sec1p and is enriched in microsomal membrane fractions; required for sporulation" * Jamie_Mitco_SPB_61.21676.21676.2 1.6128 0.0618 87.8 2487.0522 2488.7922 8291.0 41 3.195 26.3 1 K.HDMKKQNSRHFSPSFSNRRK.A YHR172W 6 7 9.4% 823 96825 6.4 U SPC97 SGDID:S000001215, Chr VIII from 448335-450806, Verified ORF, "Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque" * Jamie_Mitco_SPB_72.25745.25745.3 3.4109 0.3392 100.0 2713.0444 2713.2363 7878.1 1 5.5 30.4 1 R.RVQEALVVKDLLNVLIGLEGTYIR.Y * Jamie_Mitco_SPB_73.26220.26220.2 3.3197 0.4139 100.0 2554.9321 2557.0488 11354.1 1 6.796 43.2 1 R.VQEALVVKDLLNVLIGLEGTYIR.Y * Jamie_Mitco_SPB_29.10120.10120.2 3.8463 0.4171 100.0 2092.2722 2092.2227 6173.9 1 6.226 53.1 2 R.YFNDYEPSDPETPIEFK.I * Jamie_Mitco_SPB_22.07859.07859.3 3.5153 0.0382 93.1 2213.2144 2210.4119 8737.7 1 3.252 36.8 1 K.DVLLRDCDSEEDKNLLFK.M * Jamie_Mitco_SPB_16.05646.05646.2 3.7386 0.207 100.0 1615.1721 1613.6844 6256.6 1 5.423 75.0 1 R.DCDSEEDKNLLFK.M * Jamie_Mitco_SPB_32.11273.11273.2 3.2322 0.1547 100.0 2210.672 2211.3525 7740.1 1 5.036 47.1 1 R.NDNNANYDKLLQNFELER.Q Reverse_YDL119C 1 1 9.4% 307 34204 9.9 U YDL119C SGDID:S000002277, Chr IV from 247612-246689, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Mitco_SPB_91.32719.32719.3 2.6626 0.0957 91.1 3177.5344 3176.56 7712.1 376 2.934 18.8 1 R.PLSSSKNYVINSDNTLSPVANRRKALSSR.M Reverse_YBR146W 1 1 9.4% 278 31925 10.3 U MRPS9 SGDID:S000000350, Chr II from 535254-536090, Verified ORF, "Mitochondrial ribosomal protein of the small subunit" * Jamie_Mitco_SPB_115.41347.41347.2 1.3292 0.2775 98.7 2946.372 2946.3923 5048.9 249 4.354 16.0 1 R.KGVAISRGFEDLT*KIKINNSLMSSKK.Y contaminant_KERATIN05 4 4 9.3% 471 51531 5.2 U no description * Jamie_Mitco_SPB_33.11746.11746.2 2.7999 0.2243 100.0 2054.0923 2055.339 6190.4 1 4.965 47.2 1 K.ILTATVDNANVLLQIDNAR.L Jamie_Mitco_SPB_18.06147.06147.2 1.9837 0.1937 99.8 1187.2122 1186.397 5968.7 54 4.282 55.6 1 R.RVLDELTLAR.A Jamie_Mitco_SPB_19.06538.06538.2 3.7409 0.304 100.0 1031.9922 1030.2096 4116.5 1 6.114 93.8 1 R.VLDELTLAR.A * Jamie_Mitco_SPB_33.11686.11686.2 4.2793 0.4156 100.0 1916.2922 1916.0538 7982.0 1 7.376 64.3 1 K.DAEEWFFTKTEELNR.E Reverse_YOR062C 1 1 9.3% 268 31880 8.2 U YOR062C SGDID:S000005588, Chr XV from 443532-442726, reverse complement, Uncharacterized ORF, "Protein of unknown function; similar to YKR075Cp and Reg1p; expression regulated by glucose and Rgt1p" * Jamie_Mitco_SPB_91.32463.32463.2 1.4534 0.2155 97.4 2855.0923 2853.2078 6744.1 30 3.751 22.9 1 R.QS*LWKGKSYFVSGKKLLSSASGEKR.V Reverse_YCL016C 2 2 9.2% 380 44074 6.5 U DCC1 SGDID:S000000521, Chr III from 95762-94620, reverse complement, Verified ORF, "Subunit of a complex with Ctf8p and Ctf18p that shares some components with Replication Factor C, required for sister chromatid cohesion" * Jamie_Mitco_SPB_46.16482.16482.2 1.5296 0.0502 86.8 1169.8722 1169.3237 4174.9 5 3.19 61.1 1 R.S*TVVTKKGLR.K * Jamie_Mitco_SPB_17.05979.05979.3 2.8914 0.0851 92.3 2813.3643 2812.1584 7925.3 17 3.992 24.0 1 K.HWKSIGELASCPSNELLEELTGIVK.T YKL085W 1 1 9.0% 334 35650 8.5 U MDH1 SGDID:S000001568, Chr XI from 278767-279771, Verified ORF, "Mitochondrial malate dehydrogenase, catalyzes interconversion of malate and oxaloacetate; involved in the tricarboxylic acid (TCA) cycle" * Jamie_Mitco_SPB_74.26399.26399.3 2.657 0.0478 84.5 3039.5645 3040.4868 7540.9 388 2.738 19.0 1 R.AFSST*VANPYKVTVLGAGGGIGQPLSLLLK.L Reverse_YBR048W 1 1 9.0% 156 17749 10.8 U RPS11B SGDID:S000000252, Chr II from 332829-332873,333385-333810, Verified ORF, "Protein component of the small (40S) ribosomal subunit; identical to Rps11Ap and has similarity to E. coli S17 and rat S11 ribosomal proteins" Reverse_YDR025W 1 1 9.0% 156 17749 10.8 U RPS11A SGDID:S000002432, Chr IV from 491511-491555,491895-492320, Verified ORF, "Protein component of the small (40S) ribosomal subunit; identical to Rps11Bp and has similarity to E. coli S17 and rat S11 ribosomal proteins" Jamie_Mitco_SPB_84.30114.30114.2 1.5227 0.0448 84.2 1713.1322 1711.7802 5574.9 4 2.945 46.2 1 K.KDIYS*GEIATKPT*K.F YKL129C 5 5 8.9% 1271 142507 9.4 U MYO3 SGDID:S000001612, Chr XI from 200163-196348, reverse complement, Verified ORF, "One of two type I myosins; localizes to actin cortical patches; deletion of MYO3 has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization" * Jamie_Mitco_SPB_58.20554.20554.2 1.586 0.0201 82.7 2575.4722 2574.5542 7105.9 29 3.169 27.3 1 K.S*Y*NENQCVIISGESGAGKTEAAK.R * Jamie_Mitco_SPB_58.20631.20631.2 1.5675 0.0215 82.7 1873.8722 1871.1501 6250.7 12 2.916 35.7 1 R.T*IREKKGGNKYVKLR.D * Jamie_Mitco_SPB_29.10146.10146.4 3.5776 0.0937 85.1 4459.0166 4464.1406 9181.8 334 3.357 17.6 1 R.RQVGIKDKVVFSIKGECLHSKFGRS*AQRLKKVFILT*K.K * Jamie_Mitco_SPB_90.32087.32087.3 2.5754 0.0584 84.9 2683.5544 2683.9358 6239.7 180 3.475 24.0 1 K.T*VPIKSSAIPAAKVSS*KHSSKPSSK.E * Jamie_Mitco_SPB_101.36090.36090.2 1.5761 0.1478 95.3 2400.7322 2398.5522 6515.4 242 3.274 27.5 1 K.HSS*KPSS*KEKVAVKKASSSHK.S YOR310C 2 2 8.8% 511 56956 8.9 U NOP58 SGDID:S000005837, Chr XV from 898355-896820, reverse complement, Verified ORF, "Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA" * Jamie_Mitco_SPB_32.11218.11218.2 2.283 0.1573 99.8 1959.7322 1961.0869 7356.9 206 3.912 34.4 1 K.ASETDLSEILPEEIEER.V * Jamie_Mitco_SPB_5.01747.01747.4 2.7151 0.1259 84.4 3190.3765 3185.4836 8325.9 378 3.294 19.1 1 K.HDTPKY*GLLYHASLVGQAT*GKNKGKIAR.V YPL179W 2 2 8.7% 549 61421 9.3 U PPQ1 SGDID:S000006100, Chr XVI from 208156-209805, Verified ORF, "Putative protein serine/threonine phosphatase; null mutation enhances efficiency of translational suppressors" * Jamie_Mitco_SPB_67.23962.23962.2 2.4603 0.2689 87.5 2099.672 2101.405 5943.1 1 4.879 35.0 1 L.DIDVSVAKALSSSATAPKLIN.T * Jamie_Mitco_SPB_38.13429.13429.3 2.4677 0.0465 80.2 2977.5842 2977.2114 8861.2 43 3.591 22.1 1 R.YSHDTSSNEGLDIDVAIEKLLQVGESR.E YAR002W 2 2 8.7% 539 59039 9.3 U NUP60 SGDID:S000000063, Chr I from 152259-153878, Verified ORF, "Subunit of the nuclear pore complex (NPC), functions to anchor Nup2p to the NPC in a dynamic process that is controlled by the nucleoplasmic concentration of Gsp1p-GTP; potential Cdc28p substrate" * Jamie_Mitco_SPB_78.28025.28025.4 3.6294 0.2529 90.0 3069.1765 3074.2456 4692.7 378 4.29 20.8 1 D.TYTANTS*AQSIASAKSVRSGVSKSAPS*KK.I * Jamie_Mitco_SPB_95.33934.33934.3 2.5333 0.0446 80.4 3344.9944 3341.6572 10314.8 1 3.398 22.6 1 K.SVRSGVSKSAPSKKISNTAAALVSLLDENDS*K.K YGR192C 1 1 8.7% 332 35747 7.0 U TDH3 SGDID:S000003424, Chr VII from 883815-882817, reverse complement, Verified ORF, "Glyceraldehyde-3-phosphate dehydrogenase, isozyme 3, involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell-wall " * Jamie_Mitco_SPB_59.21111.21111.4 3.4152 0.2655 94.7 3320.4565 3317.7078 8206.0 353 4.211 20.2 1 K.KVVITAPS*STAPMFVMGVNEEKYT*SDLKI.V Reverse_YFR010W 2 2 8.6% 499 57111 7.2 U UBP6 SGDID:S000001906, Chr VI from 165060-166559, Verified ORF, "Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; deletion causes hypersensitivity to cycloheximide and other toxic compounds" * Jamie_Mitco_SPB_94.33723.33723.2 1.6891 0.0913 91.1 2374.2722 2372.52 6243.8 45 3.037 30.0 1 K.Y*MLILASDSEGGGALS*EIKEK.E * Jamie_Mitco_SPB_62.22167.22167.2 2.0759 0.0568 94.1 2510.6921 2508.6182 8856.5 64 4.323 23.8 1 K.SKLDAS*TADT*SLTIPYVKGSHR.I Reverse_YGL119W 1 1 8.6% 501 56742 9.3 U ABC1 SGDID:S000003087, Chr VII from 284448-285953, Verified ORF, "Protein required for ubiquinone (coenzyme Q) biosynthesis and for respiratory growth; exhibits genetic interaction with COQ9, suggesting a common function; similar to prokaryotic proteins involved in early steps of ubiquinone biosynthesis" * Jamie_Mitco_SPB_64.22917.22917.4 3.3618 0.1115 85.4 4997.2964 4998.5796 8272.3 380 3.302 14.7 1 K.EDQFSLLQGIKLAVGRMKSFKNTIRDINSDS*LILSKWT*PSNGK.A YLL003W 7 7 8.4% 946 112978 9.8 U SFI1 SGDID:S000003926, Chr XII from 143200-146040, Verified ORF, "Centrin (Cdc31p)-binding protein required for spindle pole body (SPB) duplication, localizes to the half-bridge of the SPB, required for progression through G(2)-M transition, has similarity to Xenopus laevis XCAP-C" * Jamie_Mitco_SPB_30.10686.10686.2 3.887 0.3672 100.0 2194.612 2195.4314 4904.3 1 6.555 58.3 1 R.DKFVPETSPTNIPT*DVLIK.Q * Jamie_Mitco_SPB_33.11574.11574.2 4.0275 0.3807 100.0 1952.2122 1952.1687 4440.7 1 6.85 59.4 1 K.FVPETSPTNIPT*DVLIK.Q * Jamie_Mitco_SPB_39.13689.13689.2 2.6114 0.2903 100.0 2030.5521 2032.1687 7630.8 7 4.789 40.6 1 K.FVPETSPT*NIPT*DVLIK.Q * Jamie_Mitco_SPB_51.18307.18307.2 2.7652 0.0518 98.7 2037.6322 2037.1443 7896.7 1 4.36 50.0 1 R.YDLNDIS*VEVIEDLYR.Q * Jamie_Mitco_SPB_76.27007.27007.2 1.4734 0.0186 80.7 2750.0322 2749.1492 8204.7 185 3.468 26.2 1 K.LEVKLKIFEQKCKKSIQAS*AYR.T * Jamie_Mitco_SPB_14.04726.04726.2 1.4084 0.0652 86.3 959.9322 959.04944 5957.5 8 3.376 78.6 1 K.VDHIDFGR.I * Jamie_Mitco_SPB_30.10495.10495.1 1.4113 0.1525 100.0 1507.61 1508.6714 4823.3 135 4.047 38.5 1 R.IPAVPFSLS*ANSPK.I YJL041W 3 3 8.3% 823 86516 6.3 U NSP1 SGDID:S000003577, Chr X from 365700-365700,365819-368289, Verified ORF, "Essential component of the nuclear pore complex, which mediates nuclear import and export" * Jamie_Mitco_SPB_14.04750.04750.4 4.3104 0.3775 100.0 2063.6165 2062.1975 7395.5 1 6.476 35.2 1 K.SDEKKDSDSSKPAFSFGTK.S * Jamie_Mitco_SPB_88.31483.31483.3 1.9207 0.1435 87.9 4225.014 4222.393 8960.4 321 3.392 13.8 1 K.KESDESKSAFS*FGS*KPTGKEEGDGAKAAISFGAKPEEQK.S * Jamie_Mitco_SPB_5.01563.01563.2 1.5592 0.0369 85.6 1079.5122 1079.1956 7629.4 317 3.661 50.0 1 K.SSDSLKLNSK.P YKR023W 2 2 8.3% 530 60799 6.9 U YKR023W SGDID:S000001731, Chr XI from 483062-484654, Uncharacterized ORF, "Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and mitochondrion" * Jamie_Mitco_SPB_17.06113.06113.2 1.7491 0.0524 89.8 1420.5521 1421.6371 6089.0 110 2.784 50.0 1 K.KLKDEKKSSTTR.P * Jamie_Mitco_SPB_110.39407.39407.3 2.9238 0.2649 85.2 3422.3342 3425.7373 5226.4 1 4.857 23.4 1 L.NQEKNELNGSSSSLSNASNGANVPKKKTKTYK.I contaminant_SPA2_STAAU 3 3 8.3% 508 55439 5.7 U owl|P38507| IMMUNOGLOBULIN G BINDING PROTEIN A PRECURSOR (PROTEIN A). - STAPHYLOCOCCUS... * Jamie_Mitco_SPB_17.06085.06085.2 2.5413 0.0532 98.2 1462.0521 1461.5254 6487.1 24 3.477 53.8 1 K.DDPSQSTNVLGEAK.K * Jamie_Mitco_SPB_17.05919.05919.2 2.6902 0.204 100.0 1458.3922 1459.5529 7807.0 50 4.331 50.0 1 K.DDPSQSANLLAEAK.K * Jamie_Mitco_SPB_40.14248.14248.2 3.7809 0.3969 100.0 1769.4722 1768.967 5369.0 1 7.234 69.2 1 F.YEILHLPNLNEEQR.N YEL015W 2 2 8.2% 551 61340 6.4 U EDC3 SGDID:S000000741, Chr V from 126629-128284, Verified ORF, "Non-essential conserved protein of unknown function, plays a role in mRNA decapping by specifically affecting the function of the decapping enzyme Dcp1p; localizes to cytoplasmic mRNA processing bodies" * Jamie_Mitco_SPB_1.00196.00196.4 2.1123 0.156 80.6 3382.4565 3382.7122 8120.2 358 3.623 16.1 1 K.LIQGKIAKAT*SKGLTLNDVQFGDGGKS*QAFK.V * Jamie_Mitco_SPB_123.44109.44109.3 1.7998 0.125 85.0 1498.8544 1502.7544 7571.1 194 3.758 32.7 1 K.DLKVLTVASQSGKR.K YDR145W 1 1 8.0% 539 61073 9.6 U TAF12 SGDID:S000002552, Chr IV from 746733-748352, Verified ORF, "Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A" * Jamie_Mitco_SPB_42.15057.15057.4 3.4489 0.0912 84.4 4871.6167 4868.113 7659.6 267 3.201 13.5 1 K.TVGIDEGDGET*VIDGDVEELLLDLADDFVTNVTAFS*CRLAKHR.K Reverse_YGR159C 2 2 8.0% 414 44535 4.9 U NSR1 SGDID:S000003391, Chr VII from 807661-806417, reverse complement, Verified ORF, "Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ribosome biogenesis" * Jamie_Mitco_SPB_18.06153.06153.2 1.7479 0.0723 90.6 1955.5322 1953.0414 5821.9 113 3.173 28.9 1 R.GLPAT*NAGSGSPRFGGRGGR.A * Jamie_Mitco_SPB_42.15029.15029.2 1.684 0.0074 83.5 1258.0922 1258.2557 4692.0 281 3.011 45.8 1 R.GGGRGGFGRS*GGR.G Reverse_YHR203C 1 1 8.0% 261 29410 10.1 U RPS4B SGDID:S000001246, Chr VIII from 505530-505517,505247-504476, reverse complement, Verified ORF, "Protein component of the small (40S) ribosomal subunit; identical to Rps4Bp and has similarity to rat S4 ribosomal protein" Reverse_YJR145C 1 1 8.0% 261 29410 10.1 U RPS4A SGDID:S000003906, Chr X from 702983-702970,702713-701942, reverse complement, Verified ORF, "Protein component of the small (40S) ribosomal subunit; mutation affects 20S pre-rRNA processing; identical to Rps4Bp and has similarity to rat S4 ribosomal protein" Jamie_Mitco_SPB_95.34147.34147.2 1.5946 0.0606 87.4 2469.612 2467.614 7495.4 19 3.368 27.5 1 R.TVFTNDLS*DKIHVLDFGGDHR.E Reverse_YDR268W 1 1 7.9% 379 43015 9.1 U MSW1 SGDID:S000002676, Chr IV from 1003998-1005137, Verified ORF, "Mitochondrial tryptophanyl-tRNA synthetase" * Jamie_Mitco_SPB_91.32688.32688.3 2.915 0.3026 94.4 3629.8145 3632.228 10733.4 169 4.774 19.8 1 K.SMKKEPTSLSLVKKT*QALMTVPKPFFNKKY*.M Reverse_YDR479C 2 2 7.8% 554 63543 6.2 U PEX29 SGDID:S000002887, Chr IV from 1416864-1415200, reverse complement, Verified ORF, "Peroxisomal integral membrane protein, involved in regulation of peroxisome size and number; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p" * Jamie_Mitco_SPB_53.19069.19069.2 1.7756 0.0181 86.1 2487.7122 2489.4631 8459.6 191 2.964 25.0 1 R.DDDSS*HTLGGSMS*GKTVGHVGQR.Y * Jamie_Mitco_SPB_71.25249.25249.2 1.7013 0.0030 82.7 2153.4922 2153.3562 6588.2 11 3.048 34.2 1 R.LPPGQAPTQNNDLYSTPNPK.H YDL119C 1 1 7.8% 307 34204 9.9 U YDL119C SGDID:S000002277, Chr IV from 247612-246689, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Mitco_SPB_84.30054.30054.2 1.6945 0.1908 98.6 2676.892 2676.0266 8856.2 73 4.27 23.9 1 R.GTLPSALRTS*IGSALYLSCLNLMR.S Reverse_YGL120C 2 2 7.7% 767 87562 6.4 U PRP43 SGDID:S000003088, Chr VII from 283943-281640, reverse complement, Verified ORF, "RNA helicase in the DEAH-box family, involved in release of the lariat-intron from the spliceosome" * Jamie_Mitco_SPB_61.21643.21643.2 1.2359 0.1031 83.0 3326.152 3327.523 10434.4 164 2.866 20.8 1 K.RINDFYKPS*EYDTTNLELNY*RNMLR.E * Jamie_Mitco_SPB_63.22429.22429.3 2.9854 0.2578 81.5 3905.7544 3906.279 10278.7 43 3.992 18.9 1 K.QFAEETYLRFCKGPRTRGARGARQQASAKS*IPSV.L YBR270C 1 1 7.7% 545 61302 8.8 U YBR270C SGDID:S000000474, Chr II from 744393-742756, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Mitco_SPB_96.34411.34411.4 3.2162 0.1636 94.6 4788.2163 4783.305 9286.3 21 3.5 16.3 1 K.LFHTKS*NMSVNSLQSSLSTGHSHSNKGS*NVFSKMAKKLLPYK.P Reverse_YLR058C 1 1 7.7% 469 52218 7.4 U SHM2 SGDID:S000004048, Chr XII from 259402-257993, reverse complement, Verified ORF, "Cytosolic serine hydroxymethyltransferase, involved in one-carbon metabolism" * Jamie_Mitco_SPB_107.38284.38284.3 2.814 0.0306 83.2 3978.8044 3979.173 10494.1 249 3.458 17.1 1 R.AGPYGESYKNSLPTGLADFVSTS*TFNES*AILDISHK.Q YMR129W 5 5 7.6% 1337 151652 6.7 U POM152 SGDID:S000004736, Chr XIII from 527803-531816, Verified ORF, "Nuclear pore membrane glycoprotein; may be involved in duplication of nuclear pores and nuclear pore complexes during S-phase;" * Jamie_Mitco_SPB_17.06028.06028.2 1.8399 0.1214 98.3 1207.1122 1206.2139 5561.5 7 3.933 68.8 1 R.YNVFNDT*PR.G * Jamie_Mitco_SPB_68.24405.24405.3 2.8665 0.161 96.2 3456.5044 3460.823 7662.3 118 3.844 19.4 1 R.DLYTNSVELRSLS*KKDFKIIDNPKS*FLK.K * Jamie_Mitco_SPB_94.33498.33498.2 1.6485 0.0894 90.5 3460.6921 3459.9177 7453.2 5 3.638 22.4 1 R.SIAIVFEEIKNWISDIPYVISLSYTDAQDK.S * Jamie_Mitco_SPB_39.13780.13780.2 2.1544 0.0424 93.5 1926.5122 1925.1913 7492.9 2 3.316 43.8 1 R.DIPSAAFNFFEPIKEAK.I * Jamie_Mitco_SPB_30.10714.10714.2 4.2312 0.4965 100.0 2166.4722 2167.247 7308.4 1 8.78 55.9 1 R.TDQYTIDNIDSENFSFEK.L YHR114W 2 2 7.6% 633 71171 6.0 U BZZ1 SGDID:S000001156, Chr VIII from 338086-339987, Verified ORF, "SH3 domain protein implicated in the regulation of actin polymerization, able to recruit actin polymerization machinery through its SH3 domains, colocalizes with cortical actin patches and Las17p, interacts with type I myosins" * Jamie_Mitco_SPB_19.06533.06533.3 2.8241 0.0376 86.7 2327.5444 2325.3303 7445.9 433 2.904 23.8 1 R.RT*RLGSAPNNAGEDSDNNSIR.T * Jamie_Mitco_SPB_44.15510.15510.2 1.4861 0.3373 87.1 2886.912 2884.0862 10423.3 166 5.295 19.2 1 K.ISLVARDT*GSGWTKINNDTTGETGLVP.T Reverse_YJR121W 1 1 7.6% 511 54794 5.7 U ATP2 SGDID:S000003882, Chr X from 647522-649057, Verified ORF, "Beta subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis" * Jamie_Mitco_SPB_70.25173.25173.3 2.0772 0.1242 86.7 4285.9146 4285.845 11101.3 57 3.678 15.8 1 R.LKS*KIPGREDIPEGIVNIIRGLT*ERGVPVSIPGGTDLVK.E Reverse_YIL150C 2 2 7.5% 571 65815 9.7 U MCM10 SGDID:S000001412, Chr IX from 62728-61013, reverse complement, Verified ORF, "Essential, chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins" * Jamie_Mitco_SPB_47.16835.16835.3 2.447 0.1096 91.4 2558.2444 2559.669 6510.6 181 3.086 27.4 1 K.GHTPDFGLRQILGTQLASET*T*R.K * Jamie_Mitco_SPB_48.17225.17225.2 1.3624 0.0576 81.9 2612.632 2612.7876 9696.3 194 2.953 25.0 1 K.EMFYT*TT*SAPPKPQQQLQSLR.V YDL042C 2 2 7.5% 562 63262 8.5 U SIR2 SGDID:S000002200, Chr IV from 378442-376754, reverse complement, Verified ORF, "Conserved NAD+ dependent histone deacetylase of the Sirtuin family involved in regulation of lifespan; plays roles in silencing at HML, HMR, telomeres, and the rDNA locus; negatively regulates initiation of DNA replication" * Jamie_Mitco_SPB_52.18553.18553.2 1.3673 0.047 80.9 3093.132 3091.3586 7289.3 113 3.127 18.5 1 R.KILVLT*GAGVSTS*LGIPDFRSSEGFYSK.I * Jamie_Mitco_SPB_96.34353.34353.2 1.9245 0.2439 88.4 1791.7522 1789.1176 5339.2 17 4.421 42.3 1 K.IYSPLHS*FIKMLQM.K YCL054W 3 3 7.4% 841 96485 8.0 U SPB1 SGDID:S000000559, Chr III from 31449-33974, Verified ORF, "AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the putative tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants" * Jamie_Mitco_SPB_40.14383.14383.2 2.3591 0.3257 100.0 2567.912 2567.651 10336.8 2 5.792 34.1 1 K.DKDSAADADDLESELNAMYSDYK.T * Jamie_Mitco_SPB_22.07752.07752.2 4.2832 0.4592 100.0 1998.7722 1999.0111 5998.8 1 8.069 58.8 1 R.GGDNEEEWTGFNEGSLEK.K * Jamie_Mitco_SPB_46.16539.16539.2 3.0884 0.3231 100.0 2241.7522 2239.592 10105.9 1 4.899 40.0 1 R.DIDIATVEAMTLAHQLALGQK.N YCR094W 1 1 7.4% 391 44982 7.7 U CDC50 SGDID:S000000690, Chr III from 286758-287933, Verified ORF, "Endosomal protein that regulates cell polarity; similar to Ynr048wp and Lem3p" * Jamie_Mitco_SPB_16.05405.05405.4 4.3262 0.2625 92.3 3349.1365 3354.7964 6484.4 135 4.547 19.6 1 K.APPLT*KEGPTSKKPPNTAFRQQRLKAWQP.I YGR267C 1 1 7.4% 243 27769 7.2 U FOL2 SGDID:S000003499, Chr VII from 1025741-1025010, reverse complement, Verified ORF, "GTP-cyclohydrolase I, catalyzes the first step in the folic acid biosynthetic pathway" * Jamie_Mitco_SPB_70.24876.24876.2 2.2725 0.2253 91.9 2148.392 2146.3623 6451.3 56 4.443 32.4 1 E.ETEEEEKERIQRISGAIK.T YKL113C 2 2 7.3% 382 43279 8.9 U RAD27 SGDID:S000001596, Chr XI from 225519-224371, reverse complement, Verified ORF, "5' to 3' exonuclease, 5' flap endonuclease, required for Okazaki fragment processing and maturation as well as for long-patch base-excision repair; member of the S. pombe RAD2/FEN1 family" * Jamie_Mitco_SPB_27.09571.09571.2 1.938 0.0811 96.3 1476.2122 1475.5583 5754.2 6 3.67 54.5 1 K.FS*EERVKSGISR.L * Jamie_Mitco_SPB_28.09816.09816.2 2.178 0.2035 84.9 1783.5721 1785.0566 7088.2 9 4.054 36.7 1 T.KEQLAAAAKRAQENKK.L YDL229W 3 3 7.2% 613 66602 5.4 U SSB1 SGDID:S000002388, Chr IV from 44066-45907, Verified ORF, "Cytoplasmic ATPase that is a ribosome-associated molecular chaperone; may be involved in the folding of newly-synthesized polypeptide chains; member of the heat shock protein 70 (HSP70) family; interacts with the phosphatase subunit Reg1p" YNL209W 3 3 7.2% 613 66595 5.5 U SSB2 SGDID:S000005153, Chr XIV from 252060-253901, Verified ORF, "Cytoplasmic ATPase that is a ribosome-associated molecular chaperone; may be involved in the folding of newly-synthesized polypeptide chains; member of the heat shock protein 70 (HSP70) family; homolog of SSB1" Jamie_Mitco_SPB_37.13061.13061.2 4.0896 0.4889 100.0 2161.0522 2161.4138 8259.2 1 7.99 41.7 1 K.VIDVDGNPVIEVQYLEETK.T Jamie_Mitco_SPB_51.18205.18205.2 2.7246 0.083 99.4 2628.4321 2627.9084 9789.5 1 4.276 35.4 1 K.SKIEAALSDALAALQIEDPSADELR.K Jamie_Mitco_SPB_49.17431.17431.2 3.3197 0.4372 100.0 2412.5522 2412.6562 8978.2 1 8.972 43.2 1 K.IEAALSDALAALQIEDPSADELR.K contaminant_KERATIN15 6 6 7.2% 629 64511 6.5 U no description Jamie_Mitco_SPB_15.05283.05283.2 4.0462 0.0951 100.0 1479.3522 1477.7007 11408.2 1 3.823 90.9 1 R.FLEQQNKVLETK.W Jamie_Mitco_SPB_11.03904.03904.2 2.7247 0.1669 95.5 1195.0122 1195.3176 4778.7 1 5.748 93.8 1 K.KYEDEINKR.Y Jamie_Mitco_SPB_9.03005.03005.2 2.13 0.144 99.8 1067.3722 1067.1436 6192.7 305 4.211 64.3 1 K.YEDEINKR.Y * Jamie_Mitco_SPB_58.20604.20604.2 1.4636 0.0221 81.0 1443.6721 1441.6268 5064.2 43 3.209 45.5 1 K.RYAAENEFVTLK.K * Jamie_Mitco_SPB_40.14361.14361.1 2.5182 0.3214 100.0 1302.46 1303.4521 12614.8 12 6.486 54.2 1 R.SLDLDSIIAEVGA.Q * Jamie_Mitco_SPB_41.14481.14481.2 4.0943 0.2936 100.0 1304.3322 1303.4521 8058.4 1 6.478 75.0 1 R.SLDLDSIIAEVGA.Q Reverse_YGL075C 2 2 7.2% 387 44586 8.3 U MPS2 SGDID:S000003043, Chr VII from 368091-366928, reverse complement, Verified ORF, "Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required for insertion of the newly duplicated SPB into the nuclear envelope; potentially phosphorylated by Cdc28p" * Jamie_Mitco_SPB_56.20154.20154.2 1.5666 0.0501 86.0 1726.0922 1725.7666 9589.9 2 3.396 45.8 1 R.IQDKLFSIT*DT*QR.Q * Jamie_Mitco_SPB_39.13909.13909.2 1.7765 0.0824 91.9 1974.6322 1973.1503 9647.7 8 2.935 35.7 1 K.EQYTELRIKLES*VQR.E YGL078C 1 1 7.1% 523 58827 9.1 U DBP3 SGDID:S000003046, Chr VII from 361862-360291, reverse complement, Verified ORF, "Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis" * Jamie_Mitco_SPB_80.28540.28540.4 3.3924 0.1616 94.3 4162.177 4160.7173 6412.6 238 3.813 16.7 1 K.SNLLLATDVAARGLDIPNVKT*VINLTFPLTVEDYVHR.I Reverse_YHR114W 2 2 7.0% 633 71171 6.0 U BZZ1 SGDID:S000001156, Chr VIII from 338086-339987, Verified ORF, "SH3 domain protein implicated in the regulation of actin polymerization, able to recruit actin polymerization machinery through its SH3 domains, colocalizes with cortical actin patches and Las17p, interacts with type I myosins" * Jamie_Mitco_SPB_68.24144.24144.2 2.2851 0.2632 93.5 2044.6721 2044.1077 6325.0 4 4.777 36.1 1 F.LGKLGDCEGYTWGSGDDGR.I * Jamie_Mitco_SPB_53.19027.19027.2 1.9281 0.1154 97.2 2549.4722 2548.556 9045.8 7 3.248 22.9 1 R.STNFLST*IHLPGGNGNGTSGGS*SPK.S Reverse_YMR005W 1 1 7.0% 388 42336 9.7 U TAF4 SGDID:S000004607, Chr XIII from 276045-277211, Verified ORF, "TFIID subunit (48 kDa), involved in RNA polymerase II transcription initiation; potential Cdc28p substrate" * Jamie_Mitco_SPB_22.07756.07756.3 2.5668 0.0513 82.7 3020.1543 3018.2793 6794.8 217 2.955 19.2 1 K.S*ANISSNLLAEEERVDIGASFLVDQMK.N YML064C 1 1 6.9% 245 27296 9.4 U TEM1 SGDID:S000004529, Chr XIII from 145876-145139, reverse complement, Verified ORF, "GTP-binding protein of the ras superfamily involved in termination of M-phase; controls actomyosin and septin dynamics during cytokinesis" * Jamie_Mitco_SPB_42.15076.15076.2 2.9365 0.2511 100.0 2111.4321 2111.313 7879.2 8 4.899 43.8 1 K.YDLLIDLDPEYQEQISR.T Reverse_YER176W 3 3 6.8% 1121 126970 9.4 U ECM32 SGDID:S000000978, Chr V from 541685-545050, Verified ORF, "DNA dependent ATPase/DNA helicase belonging to the Dna2p- and Nam7p-like family of helicases that is involved in modulating translation termination; interacts with the translation termination factors, localized to polysomes" * Jamie_Mitco_SPB_17.05992.05992.2 2.1774 0.117 98.6 2470.372 2471.9578 8439.7 2 3.601 40.5 1 K.WLPDGKRLVNKNGVVILGHKAR.T * Jamie_Mitco_SPB_15.05134.05134.2 2.9626 0.198 94.4 1548.6921 1547.8815 6956.7 174 4.088 41.7 1 V.LLVYKGRQISLEK.I * Jamie_Mitco_SPB_48.17129.17129.3 2.3922 0.0819 85.3 4666.6143 4668.7764 6029.8 1 3.879 19.4 1 R.NRSKT*ISTTNENPSISPERSSETDS*GITWRSTGKNPQNNSK.K YCL016C 1 1 6.8% 380 44074 6.5 U DCC1 SGDID:S000000521, Chr III from 95762-94620, reverse complement, Verified ORF, "Subunit of a complex with Ctf8p and Ctf18p that shares some components with Replication Factor C, required for sister chromatid cohesion" * Jamie_Mitco_SPB_59.21131.21131.3 3.018 0.0545 90.1 3212.3342 3212.518 8139.1 164 3.27 21.0 1 R.GY*HFKPT*DKTVQYIAKSTLPMDPKER.F YDR259C 2 2 6.8% 383 43597 8.7 U YAP6 SGDID:S000002667, Chr IV from 975776-974625, reverse complement, Verified ORF, "Putative basic leucine zipper (bZIP) transcription factor; overexpression increases sodium and lithium tolerance" * Jamie_Mitco_SPB_105.37543.37543.2 1.6834 0.0428 87.8 1565.9321 1566.5791 3307.3 45 2.802 45.8 1 K.DT*QLISSSGKT*LR.N * Jamie_Mitco_SPB_12.04222.04222.2 1.7841 0.0151 87.1 1553.3121 1553.7118 5929.4 74 3.689 45.8 1 K.IFDDLLAENNNFK.S YDR155C 1 1 6.8% 162 17391 7.5 U CPR1 SGDID:S000002562, Chr IV from 768995-768507, reverse complement, Verified ORF, "Cytoplasmic peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds the drug cyclosporin A" * Jamie_Mitco_SPB_29.10433.10433.2 1.8962 0.2847 90.2 1290.3722 1290.4618 8180.6 18 4.881 50.0 1 N.DIVPKTAENFR.A Reverse_YNL265C 1 1 6.7% 298 34496 6.0 U IST1 SGDID:S000005209, Chr XIV from 145282-145256,145150-144281, reverse complement, Verified ORF, "Putative translation initiation factor, as suggested by computational analysis of large-scale protein-protein interaction data" * Jamie_Mitco_SPB_20.07111.07111.3 2.4053 0.0978 90.0 2455.7644 2455.6938 6255.5 11 2.936 28.9 1 K.NT*LLLQAVQRRS*QKAIAQQK.E YDL014W 1 1 6.7% 327 34465 10.2 U NOP1 SGDID:S000002172, Chr IV from 427361-428344, Verified ORF, "Nucleolar protein, component of the small subunit processome complex, which is required for processing of pre-18S rRNA; has similarity to mammalian fibrillarin" * Jamie_Mitco_SPB_20.07188.07188.4 2.9792 0.1463 89.3 2261.1365 2264.403 4176.9 79 3.484 29.4 1 -.MSFRPGS*RGGSRGGSRGGFGGR.G YDR003W 1 2 6.7% 210 24211 5.3 U RCR2 SGDID:S000002410, Chr IV from 454119-454751, Verified ORF, "Probable integral membrane protein with similarity to Rcr1p; C-terminal region can functionally replace the corresponding region of Rcr1p in conferring Congo Red resistance when overexpressed; localizes to vacuole in a large-scale study" * Jamie_Mitco_SPB_49.17385.17385.1 1.8961 0.2062 100.0 1463.96 1464.5779 3956.5 1 4.37 42.3 2 E.SSSFDVNNAPARAK.V Reverse_YJL051W 2 2 6.6% 822 91256 6.7 U YJL051W SGDID:S000003587, Chr X from 339703-342171, Uncharacterized ORF, "Protein of unknown function, localized to the bud tip; mRNA is targeted to the bud via the mRNA transport system involving She2p" * Jamie_Mitco_SPB_54.19163.19163.2 1.8423 0.0073 86.3 1937.9122 1935.8729 6829.8 31 2.658 34.4 1 R.SNGS*TSKTPSHSSQLT*R.V * Jamie_Mitco_SPB_87.31059.31059.4 3.3645 0.0962 84.3 4223.4165 4221.5967 7491.4 200 3.286 17.1 1 K.DTVRLCAPLLASNIRGIAERELELNPSDITHS*DHPTK.Y YBR158W 1 1 6.6% 549 62686 9.1 U AMN1 SGDID:S000000362, Chr II from 556543-558192, Verified ORF, "Protein required for daughter cell separation, multiple mitotic checkpoints, and chromosome stability; contains 12 degenerate leucine-rich repeat motifs; expression is induced by the Mitotic Exit Network (MEN)" * Jamie_Mitco_SPB_86.30747.30747.4 3.733 0.227 82.1 4042.3364 4044.3403 4547.2 91 3.796 19.0 1 K.YGGHLHQSSYNPGSSKVELVRPDLSLKT*DQSFLQSS.V Reverse_YCR091W 2 2 6.5% 720 81494 8.5 U KIN82 SGDID:S000000687, Chr III from 274400-276562, Verified ORF, "Putative serine/threonine protein kinase, most similar to cyclic nucleotide-dependent protein kinase subfamily and the protein kinase C subfamily" * Jamie_Mitco_SPB_112.40255.40255.2 2.1837 0.2242 88.2 2091.0923 2092.3135 6339.8 7 4.685 38.2 1 R.RPEVIDGLSPPQKLAIY*D.E * Jamie_Mitco_SPB_33.11853.11853.3 2.881 0.0548 88.5 3574.6443 3571.9683 7688.4 209 2.739 17.9 1 R.LNRAFIKPLSMSRGKS*LRQSPSRYEQQT*M.- YOL041C 1 1 6.5% 459 51942 9.4 U NOP12 SGDID:S000005401, Chr XV from 252644-251265, reverse complement, Verified ORF, "Nucleolar protein, required for pre-25S rRNA processing; contains an RNA recognition motif (RRM) and has similarity to Nop13p, Nsr1p, and putative orthologs in Drosophila and S. pombe" * Jamie_Mitco_SPB_16.05581.05581.3 3.32 0.2087 99.4 3202.6443 3204.4663 7461.6 1 4.992 23.3 1 K.LLNEEAEAEDDKPTVTKTDETSVPLTSAAK.K YPR108W 1 1 6.5% 429 48959 5.2 U RPN7 SGDID:S000006312, Chr XVI from 742452-743741, Verified ORF, "Essential, non-ATPase regulatory subunit of the 26S proteasome, similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits" * Jamie_Mitco_SPB_60.21397.21397.2 3.2431 0.4454 100.0 3227.0522 3228.4065 7475.3 1 7.469 35.2 1 K.LEEDDEGELEQAQAWINLGEYYAQIGDK.D Reverse_YPL167C 4 4 6.4% 1504 172956 8.6 U REV3 SGDID:S000006088, Chr XVI from 237106-232592, reverse complement, Verified ORF, "Subunit of DNA polymerase zeta, which is involved in DNA repair; required for mutagenesis induced by DNA damage" * Jamie_Mitco_SPB_20.07127.07127.2 2.2499 0.0838 97.6 1895.3722 1895.83 6579.6 198 3.368 33.3 1 K.TLEEGCNCCT*ASTGVR.T * Jamie_Mitco_SPB_47.16633.16633.4 3.6459 0.0542 80.0 4319.2563 4315.8213 8441.7 305 2.897 16.2 1 K.KNGMSPVKNYWKQVADYT*PPKLAY*KWSSFMDFTPK.N * Jamie_Mitco_SPB_40.14347.14347.3 2.7477 0.0595 86.4 3450.2043 3450.8535 8215.5 175 2.867 18.8 1 K.NLIDQY*GFPPEGY*VFARKGYRLGAAMVKK.R * Jamie_Mitco_SPB_10.03366.03366.3 2.5414 0.0634 89.0 1825.7043 1828.0359 5425.8 167 2.896 35.0 1 R.SLLSTKNASVSS*RLKR.K YOR198C 2 2 6.4% 470 54639 9.2 U BFR1 SGDID:S000005724, Chr XV from 720065-718653, reverse complement, Verified ORF, "Component of mRNP complexes associated with polyribosomes; implicated in secretion and nuclear segregation; multicopy suppressor of BFA (Brefeldin A) sensitivity" * Jamie_Mitco_SPB_49.17297.17297.2 1.7668 0.0233 86.8 2049.372 2048.2607 9295.5 292 3.278 32.4 1 K.NILPDLSSNALET*KPARK.V * Jamie_Mitco_SPB_18.06360.06360.2 2.8043 0.2408 100.0 1398.3722 1397.6982 6214.5 6 5.075 54.2 1 R.KVVADDLVLVTPK.K Reverse_YPR004C 1 1 6.4% 344 36803 8.5 U YPR004C SGDID:S000006208, Chr XVI from 565038-564004, reverse complement, Verified ORF, "Electron transfer flavoprotein complex subunit ETF-alpha; contains a FAD binding domain; interacts with YFH1, the yeast frataxin homolog" * Jamie_Mitco_SPB_119.42777.42777.2 1.2981 0.1558 89.9 2410.4922 2410.6438 9573.5 76 3.447 26.2 1 R.GGTVVNQASTLEPRES*KTLITK.V Reverse_YLR029C 1 1 6.4% 204 24422 11.4 U RPL15A SGDID:S000004019, Chr XII from 202591-201977, reverse complement, Verified ORF, "Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl15Bp and has similarity to rat L15 ribosomal protein; binds to 5.8 S rRNA" * Jamie_Mitco_SPB_15.05346.05346.2 1.851 0.0151 87.9 1799.7322 1798.0145 5425.6 141 2.951 41.7 1 R.WLS*LTNQRKWTKR.R YJL129C 3 3 6.3% 1235 141073 8.5 U TRK1 SGDID:S000003665, Chr X from 177224-173517, reverse complement, Verified ORF, "Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter" * Jamie_Mitco_SPB_81.28891.28891.4 3.7934 0.1761 96.7 3984.0166 3985.2666 6700.9 147 3.904 19.3 1 K.TKSFDDNRSRAVPMERS*RT*IDMAEAKDLNELAR.T * Jamie_Mitco_SPB_30.10765.10765.2 1.704 0.0034 83.0 2348.7922 2351.5815 6896.0 269 3.299 26.3 1 K.KTENPNEIST*KSFIGAHLRK.Q * Jamie_Mitco_SPB_40.14161.14161.3 2.5913 0.0505 83.8 3085.3442 3089.4185 8771.4 128 2.875 20.8 1 K.RQARTNTEDPMTEHFKRSFTDVKHR.W contaminant_GR78_MOUSE 2 2 6.3% 655 72421 5.2 U owl|P20029| 78 KD GLUCOSE REGULATED PROTEIN PRECURSOR (GRP 78) (IMMUNOGLOBULIN... * Jamie_Mitco_SPB_63.22420.22420.2 1.9388 0.1616 98.7 1517.8922 1514.8949 3766.9 3 4.123 42.9 1 K.FTVVAAALLLLGAVR.A * Jamie_Mitco_SPB_33.11857.11857.4 3.4727 0.1073 86.8 3260.2566 3257.5383 8329.2 310 4.253 23.3 1 K.FAEEDKKLKERIDTRNELES*YAYSLK.N YDR479C 1 1 6.3% 554 63543 6.2 U PEX29 SGDID:S000002887, Chr IV from 1416864-1415200, reverse complement, Verified ORF, "Peroxisomal integral membrane protein, involved in regulation of peroxisome size and number; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p" * Jamie_Mitco_SPB_98.35221.35221.4 3.4309 0.104 85.5 4315.4966 4315.598 6777.1 49 3.335 18.6 1 R.RMWTNSCVRKKLDS*GISS*NLGEEEVVNPLREETYR.Q Reverse_YMR217W 1 1 6.3% 525 58482 6.5 U GUA1 SGDID:S000004830, Chr XIII from 701789-703366, Verified ORF, "GMP synthase, an enzyme that catalyzes the second step in the biosynthesis of GMP from inosine 5'-phosphate (IMP); transcription is not subject to regulation by guanine but is negatively regulated by nutrient starvation" * Jamie_Mitco_SPB_57.20431.20431.3 2.4562 0.1196 91.7 3674.5144 3670.2104 6415.5 16 3.36 19.5 1 K.KEPDT*VGKLKSLFEESADVVMLNIGLGEVLTKK.V YER052C 1 1 6.3% 527 58110 6.7 U HOM3 SGDID:S000000854, Chr V from 257957-256374, reverse complement, Verified ORF, "Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis" * Jamie_Mitco_SPB_83.29587.29587.3 3.6326 0.2255 87.5 3692.0942 3693.2446 11219.6 1 4.246 21.1 1 F.TILDKYKLVVDLISTSEVHVS*MALPIPDADSLK.S Reverse_contaminant_SPA2_STAAU 2 2 6.3% 508 55439 5.7 U owl|P38507| IMMUNOGLOBULIN G BINDING PROTEIN A PRECURSOR (PROTEIN A). - STAPHYLOCOCCUS... * Jamie_Mitco_SPB_70.25091.25091.2 1.9864 0.0484 92.4 1543.7522 1541.5254 4303.8 178 3.474 46.2 1 K.AEGLVNTSQS*PDDK.L * Jamie_Mitco_SPB_77.27697.27697.2 1.4329 0.0401 81.2 2097.2722 2098.1936 6895.0 11 3.295 35.3 1 K.NFKNQQADAKPAQS*DNLK.Q YKL114C 2 2 6.3% 367 41439 8.2 U APN1 SGDID:S000001597, Chr XI from 224099-222996, reverse complement, Verified ORF, "Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; controls spontaneous mutations" * Jamie_Mitco_SPB_91.32537.32537.2 1.6444 0.0303 86.8 1477.2522 1476.54 4007.1 53 3.031 50.0 1 K.EYKEKNDT*LQK.L * Jamie_Mitco_SPB_11.03631.03631.2 2.4504 0.0539 98.4 1521.0721 1520.769 4200.0 6 3.038 50.0 1 R.KEQLDKFEVKQK.K Reverse_YKL148C 1 1 6.2% 640 70229 7.5 U SDH1 SGDID:S000001631, Chr XI from 171134-169212, reverse complement, Verified ORF, "Flavoprotein subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone" * Jamie_Mitco_SPB_42.14904.14904.4 3.2267 0.1528 93.0 4505.3364 4510.9062 5034.1 255 3.471 15.4 1 R.AHAGRSEKRNAASVATQSACTLLNQLELTEVLDSNWIMS*R.D YJR002W 2 2 6.2% 593 66953 4.7 U MPP10 SGDID:S000003762, Chr X from 438779-440560, Verified ORF, "Component of the SSU processome, which is required for pre-18S rRNA processing, interacts with and controls the stability of Imp3p and Imp4p, essential for viability; similar to human Mpp10p" * Jamie_Mitco_SPB_31.10954.10954.2 3.4532 0.4407 100.0 1949.1721 1949.0813 5208.9 1 7.099 70.0 1 K.DRPEDALLTEELEFDR.T * Jamie_Mitco_SPB_57.20373.20373.4 4.5367 0.1197 95.9 4248.6567 4246.7305 8819.6 2 4.188 17.6 1 K.DRPEDALLTEELEFDRTAKPVPVITSEVTESLEDMIR.R YJL165C 2 2 6.1% 855 95455 8.4 U HAL5 SGDID:S000003701, Chr X from 109454-106887, reverse complement, Verified ORF, "Putative protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disruption increases cation and low pH sensitivity and impairs potassium uptake, suggesting a role in regulation of Trk1p and/or Trk2p transporters" * Jamie_Mitco_SPB_21.07329.07329.2 1.5853 0.0388 85.1 1570.6721 1571.7311 7768.0 228 2.812 41.7 1 K.S*QTLKKSRTSNLK.N * Jamie_Mitco_SPB_25.08919.08919.3 2.5273 0.1458 93.7 4209.4146 4205.73 4710.0 1 3.429 19.7 1 K.NGNNDELMKNDGKNIPQIVNPNAAVGVEELKLINALSEK.I contaminant_KERATIN07 3 3 6.1% 473 50915 5.5 U no description * Jamie_Mitco_SPB_30.10504.10504.3 3.8978 0.2541 100.0 2066.0344 2065.3774 8004.0 1 5.016 44.4 1 K.IIAATIENAQPILQIDNAR.L Jamie_Mitco_SPB_18.06147.06147.2 1.9837 0.1937 99.8 1187.2122 1186.397 5968.7 54 4.282 55.6 1 C.RVLDELTLAR.T Jamie_Mitco_SPB_19.06538.06538.2 3.7409 0.304 100.0 1031.9922 1030.2096 4116.5 1 6.114 93.8 1 R.VLDELTLAR.T YKR098C 1 1 6.0% 717 82703 8.4 U UBP11 SGDID:S000001806, Chr XI from 634817-632664, reverse complement, Verified ORF, "Ubiquitin-specific protease that cleaves ubiquitin from ubiquitinated proteins" * Jamie_Mitco_SPB_94.33778.33778.4 3.5305 0.098 84.8 4613.9766 4608.8853 6627.6 392 3.246 16.7 1 K.LSTGKDALFKT*LSS*PATAPPVHSLEVSSQIRDSSQDSSSSLSK.V contaminant_KERATIN08 3 3 6.0% 469 50499 5.0 U no description Jamie_Mitco_SPB_18.06147.06147.2 1.9837 0.1937 99.8 1187.2122 1186.397 5968.7 54 4.282 55.6 1 R.RVLDELTLAR.T Jamie_Mitco_SPB_19.06538.06538.2 3.7409 0.304 100.0 1031.9922 1030.2096 4116.5 1 6.114 93.8 1 R.VLDELTLAR.T * Jamie_Mitco_SPB_48.16955.16955.2 2.6958 0.1978 100.0 2152.1921 2152.4675 11290.0 6 5.242 32.4 1 R.TDLEMQIEGLKEELAYLR.K Reverse_YOR038C 2 2 5.9% 875 98445 8.3 U HIR2 SGDID:S000005564, Chr XV from 405387-402760, reverse complement, Verified ORF, "Non-essential transcriptional corepressor involved in the cell cycle-regulated transcription of histone H2A, H2B, H3, and H4 genes; recruits Swi-Snf complexes to histone gene promoters; promotes heterochromatic gene silencing with Asf1p" * Jamie_Mitco_SPB_36.12615.12615.2 1.6248 0.0115 83.7 1363.1122 1360.4702 4615.7 88 3.07 45.0 1 K.KKT*PKKGVAS*R.G * Jamie_Mitco_SPB_81.29139.29139.4 3.8661 0.3127 90.0 4931.4966 4926.5786 8456.6 7 4.609 16.7 1 K.S*AKDITIESLKDFT*KHRFLLIKNENSLLVFLIGLNIDYKVD.T YDL246C 1 1 5.9% 357 38097 6.7 U SOR2 SGDID:S000002405, Chr IV from 9756-8683, reverse complement, Uncharacterized ORF, "Protein of unknown function, computational analysis of large-scale protein-protein interaction data suggests a possible role in fructose or mannose metabolism" YJR159W 1 1 5.9% 357 38166 6.9 U SOR1 SGDID:S000003920, Chr X from 735959-737032, Verified ORF, "Sorbitol dehydrogenase; expression is induced in the presence of sorbitol" Jamie_Mitco_SPB_40.14284.14284.2 1.6649 0.0221 84.3 2320.0122 2317.6067 5931.9 22 2.974 27.5 1 R.YSFGDYRDAVNLVATGKVNVK.P YKR095W 8 10 5.8% 1875 218454 5.2 U MLP1 SGDID:S000001803, Chr XI from 619447-625074, Verified ORF, "Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved with Tel1p in telomere length control; involved with Pml1p and Pml39p in nuclear retention of unspliced mRNAs" * Jamie_Mitco_SPB_12.04230.04230.2 1.8694 0.2597 100.0 2189.7722 2191.3599 4448.2 83 5.18 38.2 1 R.KLDDLTEEKKETQSNQQR.T * Jamie_Mitco_SPB_15.05242.05242.2 3.3011 0.3554 100.0 1952.7122 1951.9928 5400.8 1 5.96 52.9 1 R.LSSITDEADEDNENLSAK.S * Jamie_Mitco_SPB_34.11961.11961.3 4.4211 0.3363 100.0 3208.8245 3208.544 6380.1 1 5.386 29.6 1 R.IEELQKELEELKTSVPNEDASYSNVTIK.Q * Jamie_Mitco_SPB_9.02928.02928.1 1.5658 0.0712 97.4 1033.31 1034.1112 5117.4 1 3.627 62.5 1 R.IEDLSSQNK.L * Jamie_Mitco_SPB_18.06243.06243.2 1.8435 0.1794 99.8 983.6122 984.1869 2556.4 55 4.213 78.6 1 K.LNQLNLLR.E * Jamie_Mitco_SPB_19.06490.06490.2 2.2402 0.2038 100.0 2078.8523 2080.2993 6615.4 32 4.272 40.0 2 R.KKEELEKEFEEKVEER.I * Jamie_Mitco_SPB_22.07831.07831.3 3.1766 0.0637 93.7 2081.8743 2080.2993 8273.8 4 4.145 40.0 2 R.KKEELEKEFEEKVEER.I * Jamie_Mitco_SPB_8.02863.02863.3 2.2077 0.0845 89.3 1485.1743 1486.7146 6792.6 24 3.325 38.6 1 R.DKLRAEIESRLR.E Reverse_YGL026C 1 1 5.8% 707 76626 6.5 U TRP5 SGDID:S000002994, Chr VII from 448540-446417, reverse complement, Verified ORF, "Tryptophan synthase involved in tryptophan biosynthesis, regulated by the general control system of amino acid biosynthesis" * Jamie_Mitco_SPB_22.07920.07920.4 3.0558 0.1985 95.7 4450.4565 4455.836 9453.0 2 3.693 17.5 1 R.RVDEAGMFVTCT*LGFKACATATAVGHQGAGT*EAIVNKKGLR.K YOL115W 2 2 5.8% 584 66031 5.5 U PAP2 SGDID:S000005475, Chr XV from 101474-103228, Verified ORF, "Catalytic subunit of TRAMP (Trf4/Pap2p-Mtr4p-Air1p/2p), a nuclear poly (A) polymerase complex involved in RNA quality control; catalyzes polyadenylation of unmodified tRNAs, and snoRNA and rRNA precursors; disputed role as a DNA polymerase" * Jamie_Mitco_SPB_14.04869.04869.1 1.2116 0.0896 95.6 1282.05 1282.3091 5583.2 115 3.619 38.9 1 R.EWLDDT*PGLR.E * Jamie_Mitco_SPB_44.15673.15673.2 1.7333 0.1287 96.3 2930.0322 2931.3242 8930.7 20 4.041 23.9 1 R.DFKDERGLVLNKAIIENENYHKKR.S Reverse_YNR035C 1 1 5.8% 342 39566 6.8 U ARC35 SGDID:S000005318, Chr XIV from 694049-693021, reverse complement, Verified ORF, "Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin" * Jamie_Mitco_SPB_114.40734.40734.2 1.4108 0.0263 80.5 2396.8523 2396.5366 5995.9 20 3.348 28.9 1 K.LLT*LSGDLQCESVSVWAKT*K.V Reverse_YFR034C 1 1 5.8% 312 34089 7.8 U PHO4 SGDID:S000001930, Chr VI from 225946-225008, reverse complement, Verified ORF, "Basic helix-loop-helix (bHLH) transcription factor; binds cooperatively with Pho2p to the PHO5 promoter; function is regulated by phosphorylation at multiple sites and by phosphate availability" * Jamie_Mitco_SPB_82.29283.29283.2 1.8874 0.0064 87.1 2121.0522 2122.1414 5056.1 19 3.026 35.3 1 K.PELDDVFGHIGEST*T*RGM.- Reverse_YER164W 3 3 5.7% 1468 168241 6.5 U CHD1 SGDID:S000000966, Chr V from 505387-509793, Verified ORF, "Nucleosome remodeling factor that functions in regulation of transcription elongation; contains a chromo domain, a helicase domain and a DNA-binding domain; component of both the SAGA and SILK complexes" * Jamie_Mitco_SPB_81.28925.28925.4 3.8946 0.0762 84.3 4432.8164 4436.0312 6323.3 48 3.448 15.9 1 K.SGIDASPSKTNLGGRLFSLLYDVRRGLHIAGPVKKSS*GTIGK.G * Jamie_Mitco_SPB_22.07583.07583.2 1.4528 0.0292 80.9 2499.612 2498.5774 6535.1 7 2.63 28.3 1 K.KS*S*GTIGKGKKSPTPTTDSSSASK.K * Jamie_Mitco_SPB_119.42796.42796.3 2.7329 0.1706 95.7 2858.3342 2860.6658 7219.7 227 3.743 23.0 1 R.RRSSRSTSDDDS*DSNAATGDGNVS*NK.I YJL034W 2 2 5.7% 682 74468 4.9 U KAR2 SGDID:S000003571, Chr X from 381243-383291, Verified ORF, "ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p" contaminant_GR78_YEAST 2 2 5.7% 682 74468 4.9 U owl|P16474| 78 KD GLUCOSE REGULATED PROTEIN HOMOLOG PRECURSOR (GRP 78) (IMMUNOGLOBULIN... Jamie_Mitco_SPB_8.02673.02673.2 1.9356 0.1341 98.9 1019.3722 1018.1582 3809.6 81 3.948 68.8 1 S.ITITNDKGR.L Jamie_Mitco_SPB_32.11187.11187.2 3.5332 0.4278 100.0 3273.372 3272.1096 8337.2 1 7.429 34.5 1 K.LYGGADGSGAADYDDEDEDDDGDYFEHDEL.- Reverse_YPL269W 1 1 5.7% 644 74245 8.6 U KAR9 SGDID:S000006190, Chr XVI from 33013-34947, Verified ORF, "Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase" * Jamie_Mitco_SPB_68.24156.24156.3 2.1103 0.262 98.6 4144.824 4144.606 8270.1 158 4.138 16.0 1 K.NPS*EPDFFPNIANSSNPST*APKINMKQLLAKGLKKGK.L Reverse_YPR163C 1 1 5.7% 436 48522 5.3 U TIF3 SGDID:S000006367, Chr XVI from 869951-868641, reverse complement, Verified ORF, "Translation initiation factor eIF-4B, has RNA annealing activity; contains an RNA recognition motif and binds to single-stranded RNA" * Jamie_Mitco_SPB_55.19481.19481.3 2.6615 0.1584 94.1 2516.3943 2515.4885 5639.9 54 3.411 22.9 1 R.DS*S*GGGLAPDLRSRGGFSGGFGGSR.T YHR211W 2 2 5.6% 1075 111981 4.3 U FLO5 SGDID:S000001254, Chr VIII from 525392-528619, Verified ORF, "Lectin-like protein involved in flocculation, cell wall protein that binds to mannose chains on the surface of other cells, confers floc-forming ability that is chymotrypsin resistant but heat labile; similar to Flo1p" * Jamie_Mitco_SPB_80.28487.28487.4 3.2273 0.3058 93.3 4495.9766 4500.4277 8249.9 30 2.168 15.9 1 S.T*SIFSESSTSSVIPTS*SSTSGSSESKTSSASSSSSSSSISSESPK.S Jamie_Mitco_SPB_98.35076.35076.2 1.4424 0.0365 81.0 1738.9321 1740.7533 5747.8 76 2.625 39.3 1 M.SLTSS*GLSTMS*QQPR.S Reverse_YER028C 1 1 5.6% 394 43119 9.9 U MIG3 SGDID:S000000830, Chr V from 211875-210691, reverse complement, Verified ORF, "Probable transcriptional repressor involved in response to toxic agents such as hydroxyurea that inhibit ribonucleotide reductase; phosphorylation by Snf1p or the Mec1p pathway inactivates Mig3p, allowing induction of damage response genes" * Jamie_Mitco_SPB_53.18887.18887.2 1.5988 0.0497 85.9 2553.2322 2555.7214 6772.7 13 3.012 28.6 1 R.LISKIPPLSVNPS*TNSARS*EQR.H YLR450W 3 3 5.5% 1045 115692 7.6 U HMG2 SGDID:S000004442, Chr XII from 1032623-1035760, Verified ORF, "One of two isozymes of HMG-CoA reductase that convert HMG-CoA to mevalonate, a rate-limiting step in sterol biosynthesis; overproduction induces assembly of peripheral ER membrane arrays and short nuclear-associated membrane stacks" * Jamie_Mitco_SPB_7.02358.02358.1 1.0765 0.2988 100.0 1287.48 1288.4406 3865.8 288 4.588 31.8 1 K.IPTELVSENGTK.W * Jamie_Mitco_SPB_43.15335.15335.3 2.3198 0.0621 80.5 3769.9143 3769.1687 8368.9 190 2.508 16.9 1 K.IGSKFWLSFSALSNS*ACALY*LSLYTTHSLLKK.P * Jamie_Mitco_SPB_62.22199.22199.2 1.882 0.2323 82.3 1440.2922 1442.7411 4700.6 16 4.503 45.8 1 K.LLFFAFAVSISIN.V YOR204W 2 2 5.5% 604 65553 7.8 U DED1 SGDID:S000005730, Chr XV from 722911-724725, Verified ORF, "ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility" * Jamie_Mitco_SPB_14.04811.04811.2 2.7819 0.2988 100.0 1658.5122 1658.7896 7013.0 1 7.061 61.5 1 R.HIVEDCDMTPVGER.Q * Jamie_Mitco_SPB_121.43421.43421.2 1.5099 0.0289 82.5 2009.8722 2007.0463 7074.0 98 2.888 30.6 1 R.DY*RKAGGASAGGWGSSRSR.D Reverse_YBR270C 2 2 5.5% 545 61302 8.8 U YBR270C SGDID:S000000474, Chr II from 744393-742756, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Mitco_SPB_122.43897.43897.2 1.3898 0.1701 95.6 1032.0521 1033.1753 7503.3 95 4.123 50.0 1 K.THFLKSGSR.S * Jamie_Mitco_SPB_65.23254.23254.2 1.7652 0.0494 89.0 2384.0122 2383.4253 8862.3 5 3.234 30.0 1 R.KNGRSNSSFLS*DNSTTNGEMR.N Reverse_YPL118W 1 1 5.5% 344 39445 10.0 U MRP51 SGDID:S000006039, Chr XVI from 326627-327661, Verified ORF, "Mitochondrial ribosomal protein of the large subunit; MRP51 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences" * Jamie_Mitco_SPB_87.31320.31320.2 2.3145 0.1923 99.8 2097.612 2095.4277 6137.5 1 4.732 44.4 1 K.HNNAVQAIRSRGLLEALTM.- Reverse_YHR082C 2 2 5.4% 1029 117082 6.6 U KSP1 SGDID:S000001124, Chr VIII from 271550-268461, reverse complement, Verified ORF, "Nonessential putative serine/threonine protein kinase of unknown cellular role; overproduction causes allele-specific suppression of the prp20-10 mutation" * Jamie_Mitco_SPB_85.30295.30295.2 1.9159 0.1071 96.5 2355.0522 2355.2942 6670.5 54 3.409 27.5 1 R.EDPS*TS*SSENDGGEMKGHAMK.D * Jamie_Mitco_SPB_39.13841.13841.4 3.5859 0.1158 87.6 4234.2163 4229.6333 6770.7 4 3.533 18.6 1 K.DS*QNAIS*FPNKHFVINLFVIGMAWLDVKACDYPEK.R YPR029C 2 2 5.4% 832 93624 5.5 U APL4 SGDID:S000006233, Chr XVI from 626964-624466, reverse complement, Verified ORF, "Gamma-adaptin, large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in vesicle mediated transport" * Jamie_Mitco_SPB_71.25561.25561.3 2.2188 0.088 84.7 2640.1443 2643.6514 6826.5 418 3.435 23.9 1 K.EES*INHS*SAVEANSGVTLPLDANK.I * Jamie_Mitco_SPB_37.13140.13140.2 1.5743 0.0314 83.3 2420.0122 2417.6401 8293.3 128 2.815 22.5 1 R.VKLDFHLNGSSSITNEQFDHK.F contaminant_KERATIN16 2 2 5.4% 534 57265 6.6 U no description Jamie_Mitco_SPB_15.05283.05283.2 4.0462 0.0951 100.0 1479.3522 1477.7007 11408.2 1 3.823 90.9 1 Q.FLEQQNKVLETK.W * Jamie_Mitco_SPB_71.25218.25218.1 1.6239 0.2252 100.0 1685.11 1684.625 4577.9 120 4.444 31.2 1 C.QSAVS*IS*VVSGSTSTGG.I Reverse_YHR168W 1 1 5.4% 499 55536 9.1 U MTG2 SGDID:S000001211, Chr VIII from 440379-441878, Verified ORF, "Putative GTPase, member of the Obg family; peripheral protein of the mitochondrial inner membrane that associates with the large ribosomal subunit; required for mitochondrial translation, possibly via a role in ribosome assembly" * Jamie_Mitco_SPB_40.14209.14209.2 2.2542 0.0306 93.9 2893.9722 2894.131 5708.6 7 3.058 25.0 1 K.KRHSGSSVS*LNVATSGLISRLTANNDK.R YPL264C 1 1 5.4% 353 39703 7.8 U YPL264C SGDID:S000006185, Chr XVI from 44344-43283, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Mitco_SPB_75.26699.26699.2 2.326 0.2375 90.7 2134.912 2134.4094 7057.5 211 4.328 27.8 1 S.MVVSTKVLENDPLETSQSR.I YIL044C 1 1 5.4% 298 32638 9.4 U AGE2 SGDID:S000001306, Chr IX from 273845-272949, reverse complement, Verified ORF, "ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector, involved in Trans-Golgi-Network (TGN) transport; contains C2C2H2 cysteine/histidine motif" * Jamie_Mitco_SPB_94.33647.33647.2 2.2081 0.292 81.8 1550.8522 1548.6963 4924.5 84 4.997 40.0 1 S.SATSIGAANTKTGNRV.G YLR029C 2 2 5.4% 204 24422 11.4 U RPL15A SGDID:S000004019, Chr XII from 202591-201977, reverse complement, Verified ORF, "Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl15Bp and has similarity to rat L15 ribosomal protein; binds to 5.8 S rRNA" * Jamie_Mitco_SPB_20.06862.06862.2 3.1617 0.3546 100.0 1370.1522 1370.548 5559.8 1 6.796 80.0 1 M.GAYKYLEELQR.K * Jamie_Mitco_SPB_14.04984.04984.2 1.739 0.13 98.4 952.15216 951.0672 4453.4 31 4.426 66.7 1 K.YLEELQR.K YIL125W 2 2 5.3% 1014 114416 7.2 U KGD1 SGDID:S000001387, Chr IX from 122689-125733, Verified ORF, "Component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes a key step in the tricarboxylic acid (TCA) cycle, the oxidative decarboxylation of alpha-ketoglutarate to form succinyl-CoA" * Jamie_Mitco_SPB_66.23699.23699.2 1.7981 0.1081 95.5 3100.2922 3101.3103 7286.8 14 3.434 23.2 1 K.SIET*GEGIDWATGEALAFGTLVLDGQNVR.V * Jamie_Mitco_SPB_38.13372.13372.3 2.5409 0.0633 84.8 2913.2344 2909.3157 7097.6 114 2.783 21.9 1 K.SIGTKEETKRLVLLSGQVY*TALHKR.R YBR142W 2 2 5.3% 773 87048 8.4 U MAK5 SGDID:S000000346, Chr II from 528311-530632, Verified ORF, "Essential nucleolar protein, putative DEAD-box RNA helicase required for maintenance of M1 dsRNA virus; involved in biogenesis of large (60S) ribosomal subunits" * Jamie_Mitco_SPB_24.08401.08401.2 3.1757 0.2575 100.0 2034.2122 2034.0919 9473.9 3 4.86 47.1 1 K.LTDPSEDVDEDVDEDVLK.E * Jamie_Mitco_SPB_121.43492.43492.3 2.3917 0.0621 82.3 2722.4644 2718.98 8329.4 52 3.037 25.0 1 K.VNTLILDEADRLLQDGHFDEFEK.I YDR432W 1 1 5.3% 414 45407 5.5 U NPL3 SGDID:S000002840, Chr IV from 1328773-1330017, Verified ORF, "RNA-binding protein that carries poly(A)+ mRNA from the nucleus into the cytoplasm; phosphorylation by Sky1p in the cytoplasm may promote release of mRNAs" * Jamie_Mitco_SPB_60.21282.21282.2 3.0857 0.3997 100.0 2437.0923 2438.6501 8572.6 1 7.067 40.5 1 R.DFDGTGALEFPSEEILVEALER.L YER176W 2 2 5.2% 1121 126970 9.4 U ECM32 SGDID:S000000978, Chr V from 541685-545050, Verified ORF, "DNA dependent ATPase/DNA helicase belonging to the Dna2p- and Nam7p-like family of helicases that is involved in modulating translation termination; interacts with the translation termination factors, localized to polysomes" * Jamie_Mitco_SPB_80.28655.28655.3 2.1981 0.1176 88.9 4609.074 4606.017 8239.7 61 3.387 16.2 1 R.IAYEVKKENSTIQSS*SSSNLRNFKGFKAVES*DPVVAAKVSK.S * Jamie_Mitco_SPB_16.05703.05703.2 1.4556 0.0525 82.9 1781.9922 1780.7599 8924.7 284 2.45 34.4 1 K.ALNGNGSGST*NT*TGLKK.G YOR363C 2 2 5.2% 996 114710 7.6 U PIP2 SGDID:S000005890, Chr XV from 1023208-1020218, reverse complement, Verified ORF, "Autoregulatory oleate-specific transcriptional activator of peroxisome proliferation, contains Zn(2)-Cys(6) cluster domain, forms heterodimer with Oaf1p, binds oleate response elements (OREs), activates beta-oxidation genes" * Jamie_Mitco_SPB_94.33528.33528.4 2.7068 0.1403 84.8 5089.3765 5090.693 8518.4 185 3.402 15.1 1 K.YLIFQDTFLKTLIASVLLSCSRNSMIPALNADIS*RS*RTQPCVK.N * Jamie_Mitco_SPB_19.06647.06647.2 1.4764 0.0040 81.0 1109.1721 1110.212 4932.0 111 3.167 56.2 1 R.KSINEFTDR.L YGL068W 1 1 5.2% 194 20650 9.2 U MNP1 SGDID:S000003036, Chr VII from 375090-375674, Verified ORF, "Protein associated with the mitochondrial nucleoid; putative mitochondrial ribosomal protein with similarity to E. coli L7/L12 ribosomal protein; required for normal respiratory growth" * Jamie_Mitco_SPB_22.07839.07839.2 1.6192 0.068 89.8 1119.5721 1119.1736 5497.2 90 3.384 50.0 1 K.ENVAKDDAEK.I YAR019C 2 2 5.1% 974 110285 8.0 U CDC15 SGDID:S000000072, Chr I from 175133-172209, reverse complement, Verified ORF, "Protein kinase of the Mitotic Exit Network that is localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p" * Jamie_Mitco_SPB_57.20482.20482.2 5.0064 0.4266 100.0 2468.3123 2467.7473 10597.8 1 8.632 57.5 1 K.EVVYENDEELNDIMAEISLLK.N * Jamie_Mitco_SPB_18.06391.06391.3 2.5799 0.1403 93.7 3008.2744 3008.1858 4578.4 2 3.796 25.0 1 K.SAIPSSSSLPLSSSPTRNS*PVNSVQSPSR.S Reverse_YOL006C 2 2 5.1% 769 89995 8.7 U TOP1 SGDID:S000005366, Chr XV from 315387-313078, reverse complement, Verified ORF, "Topoisomerase I, nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination" * Jamie_Mitco_SPB_46.16531.16531.2 1.5819 0.0123 82.0 2108.3123 2109.3618 5046.1 101 2.892 32.4 1 K.NPILDLQDQMTKSANYTR.F * Jamie_Mitco_SPB_78.28066.28066.2 1.7394 0.0146 85.0 2331.4521 2332.6597 7959.3 19 2.747 27.5 1 K.APPSPLSSSQVPETKIKKT*KK.K Reverse_YJR002W 2 2 5.1% 593 66953 4.7 U MPP10 SGDID:S000003762, Chr X from 438779-440560, Verified ORF, "Component of the SSU processome, which is required for pre-18S rRNA processing, interacts with and controls the stability of Imp3p and Imp4p, essential for viability; similar to human Mpp10p" * Jamie_Mitco_SPB_83.29775.29775.2 1.4997 0.0723 86.5 2536.4922 2535.7312 8570.9 62 3.065 23.8 1 -.LKINTS*DPGSRSKKT*KGSVDKK.E * Jamie_Mitco_SPB_12.03987.03987.2 1.8744 0.033 92.9 928.2522 930.0922 4441.4 1 3.302 78.6 1 K.QNIVTINK.A Reverse_YJL101C 1 1 5.0% 678 78254 6.3 U GSH1 SGDID:S000003637, Chr X from 236273-234237, reverse complement, Verified ORF, "Gamma glutamylcysteine synthetase, catalyzes the first step in the gamma-glutamyl cycle for glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury" * Jamie_Mitco_SPB_52.18682.18682.4 3.6502 0.0718 84.2 4281.4966 4284.7344 7392.1 166 3.229 19.2 1 K.YDPHQLVFNRFFKATTPLEGS*ARDS*ILKLYYYLR.E YFR048W 2 2 5.0% 662 75914 7.3 U RMD8 SGDID:S000001944, Chr VI from 246133-248121, Verified ORF, "Cytosolic protein required for sporulation" * Jamie_Mitco_SPB_36.12648.12648.2 1.4617 0.0384 83.0 1296.4922 1295.3951 5308.1 312 3.116 45.0 1 K.RSPS*ILVTDAR.T * Jamie_Mitco_SPB_33.11548.11548.4 3.5328 0.3112 91.7 2392.0964 2393.5725 5067.4 6 4.749 30.2 1 S.GRFSDISIDNILSDNSDIPSAR.R YGR031W 1 1 5.0% 342 38511 8.8 U YGR031W SGDID:S000003263, Chr VII from 546445-547473, Uncharacterized ORF, "Hypothetical protein" * Jamie_Mitco_SPB_120.42982.42982.2 1.6939 0.1255 95.0 1824.8322 1823.102 10729.2 40 3.937 31.2 1 K.DAIVKGEIAAWPLDPAR.E YAL019W 2 2 4.9% 1131 128507 5.4 U FUN30 SGDID:S000000017, Chr I from 114920-118315, Verified ORF, "Protein whose overexpression affects chromosome stability, potential Cdc28p substrate; homolog of Snf2p" * Jamie_Mitco_SPB_23.08050.08050.2 1.7865 0.2781 88.3 2159.372 2159.2256 6597.0 23 4.092 30.0 1 K.VAS*SSPLKPTSPT*VPDASVAS.L * Jamie_Mitco_SPB_72.25673.25673.4 3.5851 0.201 98.8 3998.8965 4001.735 6759.0 170 3.606 18.7 1 R.ANFRLLLTGTPLQNNLKELMSLLEFIMPNLFIS*K.K YGR162W 2 2 4.9% 952 107101 6.0 U TIF4631 SGDID:S000003394, Chr VII from 824064-826922, Verified ORF, "Translation initiation factor eIF4G, subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); associates with the poly(A)-binding protein Pab1p, also interacts with eIF4A (Tif1p); homologous to Tif4632p" * Jamie_Mitco_SPB_81.28833.28833.3 2.4566 0.0477 80.1 3584.1543 3584.608 6208.1 2 2.932 20.3 1 R.STVSPQPESKLKETSDSTS*TSTPTPTPST*NDSK.A * Jamie_Mitco_SPB_98.35266.35266.2 1.6179 0.0079 82.6 1708.9722 1710.8038 5886.4 1 2.956 42.3 1 R.TY*SQRNSQRAPPPK.E YER167W 2 2 4.9% 851 93738 7.9 U BCK2 SGDID:S000000969, Chr V from 518211-520766, Verified ORF, "Protein rich in serine and threonine residues involved in protein kinase C signaling pathway, which controls cell integrity; overproduction suppresses pkc1 mutations" * Jamie_Mitco_SPB_31.10858.10858.2 2.2616 0.3057 83.3 2180.892 2182.4412 6391.0 1 4.892 34.2 1 V.VPKSTHDPSLAKPPSRFTES.E * Jamie_Mitco_SPB_94.33784.33784.2 2.0791 0.2969 95.7 2437.652 2435.699 7308.9 5 4.72 26.2 1 K.YTLVNTGLGLSDANLDHFIRSQ.W YNL182C 1 1 4.9% 555 61773 5.7 U IPI3 SGDID:S000005126, Chr XIV from 297629-295962, reverse complement, Uncharacterized ORF, "Protein required for cell viability; computational analysis of large-scale protein-protein interaction data suggests a possible role in assembly of the ribosomal large subunit" * Jamie_Mitco_SPB_79.28102.28102.3 2.8792 0.1892 98.6 3015.8044 3017.363 6023.3 160 4.175 20.2 1 K.QKANENDVSIGKTPVLLATFTT*PYSIK.S YJR107W 1 1 4.9% 328 37261 6.1 U YJR107W SGDID:S000003868, Chr X from 627254-628240, Uncharacterized ORF, "Putative protein of unknown function; has sequence or structural similarity to lipases" * Jamie_Mitco_SPB_47.16708.16708.2 1.7841 0.0594 90.1 2027.8322 2030.3341 9537.8 229 3.35 30.0 1 K.ESILKDEIQFRKGYFR.V YIL156W 3 3 4.8% 1071 123134 7.9 U UBP7 SGDID:S000001418, Chr IX from 48091-51306, Verified ORF, "Ubiquitin-specific protease that cleaves ubiquitin-protein fusions" * Jamie_Mitco_SPB_12.04071.04071.2 1.7043 0.0060 84.7 1314.9321 1316.5376 6032.1 41 2.695 54.5 1 K.KEIGVVLDESVK.H * Jamie_Mitco_SPB_42.15011.15011.2 1.6079 0.0133 82.7 2657.612 2657.858 8720.5 92 2.881 22.9 1 R.CGPTASVSTSVS*ALENEPSIVKSKK.K * Jamie_Mitco_SPB_75.26704.26704.3 2.4981 0.1716 95.7 2208.3542 2211.4458 3680.6 198 3.796 34.4 1 K.S*KKKKS*RFFTLHTGTKR.R Reverse_contaminant_KERATIN13 1 1 4.8% 643 65494 6.6 U no description * Jamie_Mitco_SPB_46.16455.16455.2 1.5589 0.1021 90.2 2693.8123 2691.529 6574.6 33 3.244 21.7 1 K.VSSSGGS*S*KVGGSSSGRGGSSGGSSGGGSGR.G YGR285C 1 1 4.8% 433 49020 8.3 U ZUO1 SGDID:S000003517, Chr VII from 1063159-1061858, reverse complement, Verified ORF, "Cytosolic ribosome-associated chaperone, contains a DnaJ domain; together with Ssz1p, acts as a chaperone for nascent polypeptide chains" * Jamie_Mitco_SPB_75.26712.26712.3 2.0976 0.1493 92.0 2404.7644 2402.5464 5877.6 128 3.548 23.8 1 -.MFS*LPTLT*SDITVEVNSSATK.T YIL160C 1 1 4.8% 417 44730 7.6 U POT1 SGDID:S000001422, Chr IX from 41444-40191, reverse complement, Verified ORF, "3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids" * Jamie_Mitco_SPB_33.11643.11643.2 2.0987 0.2318 87.5 1941.8922 1943.9359 8096.9 50 4.16 28.9 1 K.DRGT*TTAGNASQVSDGVAGV.L YIL110W 1 1 4.8% 377 42515 4.7 U YIL110W SGDID:S000001372, Chr IX from 156042-157175, Uncharacterized ORF, "Putative S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family" * Jamie_Mitco_SPB_47.16633.16633.2 2.1722 0.2141 87.7 2160.132 2157.165 8441.7 153 3.879 29.4 1 K.DIYFGVGGS*ITEFEAY*LD.D YFR025C 1 1 4.8% 335 38582 5.9 U HIS2 SGDID:S000001921, Chr VI from 204738-203731, reverse complement, Verified ORF, "Histidinolphosphatase, catalyzes the eighth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control" * Jamie_Mitco_SPB_65.23392.23392.2 1.9867 0.084 96.2 1923.0922 1920.2225 6306.4 183 3.293 30.0 1 R.LEEPYPSKTLCNLVKK.H Reverse_YDL031W 3 3 4.7% 995 113158 9.3 U DBP10 SGDID:S000002189, Chr IV from 394214-397201, Verified ORF, "Putative ATP-dependent RNA helicase of the DEAD-box protein family, constituent of 66S pre-ribosomal particles; essential protein involved in ribosome biogenesis" * Jamie_Mitco_SPB_92.32776.32776.4 2.811 0.1174 83.0 3249.5764 3254.584 4760.2 106 3.908 22.0 1 R.GS*RFSAAIKQGSEGIIYKKNDIGQTNVYK.K * Jamie_Mitco_SPB_11.03784.03784.2 1.7327 0.0198 89.8 979.7322 978.1422 5043.8 56 3.095 75.0 1 K.EKKFNRR.K * Jamie_Mitco_SPB_22.07816.07816.3 2.2141 0.1388 93.6 1353.4443 1356.5823 7090.1 15 3.542 40.0 1 K.FVNFTQMALER.S YFR029W 2 2 4.7% 678 76286 7.5 U PTR3 SGDID:S000001925, Chr VI from 210925-212961, Verified ORF, "Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes" * Jamie_Mitco_SPB_85.30435.30435.2 1.4487 0.081 86.6 3134.372 3132.192 6805.4 91 3.628 19.6 1 K.SSGEYGPNFNQLTEQSSSSLST*TSQASKK.K * Jamie_Mitco_SPB_57.20175.20175.3 2.4907 0.0561 82.4 3599.4844 3596.714 10503.2 121 2.995 17.7 1 K.S*SGEYGPNFNQLT*EQSSSSLSTTSQASKKKKK.N YPL242C 3 3 4.6% 1495 172830 8.9 U IQG1 SGDID:S000006163, Chr XVI from 95109-90622, reverse complement, Verified ORF, "Essential protein required for determination of budding pattern, promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family" * Jamie_Mitco_SPB_95.33983.33983.2 1.4482 0.0111 80.3 1556.4321 1555.5529 4831.3 209 2.596 34.6 1 K.TS*AS*NSSATILSKK.V * Jamie_Mitco_SPB_78.28012.28012.3 2.7859 0.0401 84.9 3064.3442 3060.2795 6601.7 34 2.831 26.1 1 K.Y*DKLKFTLSRFSCTVELLQAY*CR.S * Jamie_Mitco_SPB_94.33541.33541.4 3.6622 0.2581 94.1 3951.8564 3948.4954 5508.3 151 4.233 18.8 1 Q.GRERS*ELQGMKFKWNTKQLYERGVLKTIRGEK.L YOR304W 2 2 4.6% 1120 130327 6.6 U ISW2 SGDID:S000005831, Chr XV from 884510-887872, Verified ORF, "Member of the imitation-switch (ISWI) class of ATP-dependent chromatin remodeling complexes; ATPase component that, with Itc1p, forms a complex required for repression of a-specific genes, INO1, and early meiotic genes during mitotic growth" * Jamie_Mitco_SPB_110.39592.39592.2 1.5817 0.0229 82.6 2164.7722 2162.027 4689.9 4 2.923 36.7 1 R.DFEY*CRIDGST*SHEER.I * Jamie_Mitco_SPB_39.13791.13791.4 4.2918 0.215 88.0 3992.2966 3993.5652 6869.5 69 4.085 17.1 1 F.GAKNMFEKKASKVTVDADIDDILKKGEQKTQELNAK.Y Reverse_YOR005C 1 1 4.6% 944 108515 8.4 U DNL4 SGDID:S000005531, Chr XV from 337343-334509, reverse complement, Verified ORF, "DNA ligase required for nonhomologous end-joining (NHEJ), forms stable heterodimer with required cofactor Lif1p, catalyzes DNA ligation as part of a complex with Lif1p and Nej1p; involved in meiosis, not essential for vegetative growth" * Jamie_Mitco_SPB_61.21640.21640.4 4.0248 0.1356 95.1 5505.4966 5499.9463 9276.4 283 3.75 14.7 1 K.RKKRILGLQSREAQYSPNSKVTRSEY*LDNLTY*CDTWEKDYRIR.K YDL122W 1 1 4.6% 809 92769 4.9 U UBP1 SGDID:S000002280, Chr IV from 242552-244981, Verified ORF, "Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains" * Jamie_Mitco_SPB_89.32004.32004.4 3.6348 0.1204 89.9 4289.2163 4292.305 7137.2 92 3.446 18.1 1 K.DIS*NYDPLNGEVDGVTSDDEDEY*IEETDALGNTIKKR.I YPL207W 2 2 4.6% 810 89805 8.2 U YPL207W SGDID:S000006128, Chr XVI from 159908-162340, Uncharacterized ORF, "Hypothetical protein" * Jamie_Mitco_SPB_18.06293.06293.2 1.5952 0.1452 96.4 1293.9321 1293.2926 2825.5 3 3.125 60.0 1 K.VFSKNSS*SNSR.V * Jamie_Mitco_SPB_68.24478.24478.3 2.9084 0.1165 94.0 2972.5444 2974.2969 7680.2 110 3.441 23.0 1 R.VDSFPLRKLVGYTVLGLGDS*ESWPEK.F Reverse_YJL128C 1 1 4.6% 668 72720 9.0 U PBS2 SGDID:S000003664, Chr X from 180021-178015, reverse complement, Verified ORF, "MAP kinase kinase that plays a pivotal role in the osmosensing signal-transduction pathway, activated under severe osmotic stress" * Jamie_Mitco_SPB_76.27010.27010.3 2.7074 0.0722 89.3 3356.2144 3355.5535 8121.7 291 3.157 18.3 1 R.SS*SGNSFDIGKSSLSLKGAFNLSGSKIDVY*K.S YLR314C 1 1 4.6% 520 60039 5.5 U CDC3 SGDID:S000004306, Chr XII from 764137-762575, reverse complement, Verified ORF, "Component of the septin ring of the mother-bud neck that is required for cytokinesis; septins recruit proteins to the neck and can act as a barrier to diffusion at the membrane, and they comprise the 10nm filaments seen with EM" * Jamie_Mitco_SPB_16.05496.05496.3 2.9155 0.1197 93.9 2724.7144 2726.6135 9541.2 427 3.623 21.7 1 K.QESQDHDGVDSQYTNGTQNDDSER.F YER014W 1 1 4.6% 539 59703 9.3 U HEM14 SGDID:S000000816, Chr V from 182599-184218, Verified ORF, "Protoporphyrinogen oxidase, a mitochondrial enzyme that catalyzes the seventh step in the heme biosynthetic pathway, converting protoporphyrinogen IX to protoporphyrin IX" * Jamie_Mitco_SPB_55.19657.19657.2 1.3129 0.069 82.1 3007.8323 3009.1326 8398.3 44 3.129 22.9 1 K.RPANETTIGLKAKS*GDQY*ETFDHLR.L YDR516C 1 1 4.6% 500 55921 6.3 U EMI2 SGDID:S000002924, Chr IV from 1476466-1474964, reverse complement, Verified ORF, "Non-essential protein of unknown function required for transcriptional induction of the early meiotic-specific transcription factor IME1, also required for sporulation" * Jamie_Mitco_SPB_34.12160.12160.3 2.5658 0.0416 81.2 2745.7144 2747.0618 8678.8 259 2.962 22.7 1 R.S*MLRHALALSPIGT*EGERKIHLR.L Reverse_YOR272W 1 1 4.6% 460 51359 7.0 U YTM1 SGDID:S000005798, Chr XV from 832810-834192, Verified ORF, "Constituent of 66S pre-ribosomal particles, required for maturation of the large ribosomal subunit" * Jamie_Mitco_SPB_46.16528.16528.3 2.8244 0.0483 88.3 2646.4143 2648.0193 8920.5 294 3.099 25.0 1 R.RRIT*GDKMTLKRRKRAATS*IK.N Reverse_YIL022W 1 1 4.6% 431 48854 9.4 U TIM44 SGDID:S000001284, Chr IX from 311163-312458, Verified ORF, "Peripheral mitochondrial membrane protein involved in mitochondrial protein import, tethers essential chaperone Ssc1p to the translocon channel at the matrix side of the inner membrane" * Jamie_Mitco_SPB_31.11135.11135.2 1.4132 0.0188 80.1 2405.0923 2407.528 8283.8 20 2.794 26.3 1 K.GLESEWAKTAIHEMT*EGT*KK.L Reverse_YBL076C 1 1 4.5% 1072 122983 6.1 U ILS1 SGDID:S000000172, Chr II from 84259-81041, reverse complement, Verified ORF, "Cytoplasmic isoleucine-tRNA synthetase, target of the G1-specific inhibitor reveromycin A" * Jamie_Mitco_SPB_81.28996.28996.4 3.0453 0.1843 94.2 5529.4165 5524.397 9016.2 86 3.828 13.8 1 R.GDKGYKALVAEPIYEKLRLY*ISESLFPTFPAMARVFTFLADFLSNLAK.L YER114C 2 2 4.5% 1040 115688 8.8 U BOI2 SGDID:S000000916, Chr V from 393708-390586, reverse complement, Verified ORF, "Protein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain" * Jamie_Mitco_SPB_107.38515.38515.4 3.4851 0.2586 90.9 3449.2166 3452.7666 4906.4 155 4.762 19.9 1 H.SSSLSSKSKSDSRRNSSLKRSSSASRTSSFKK.S * Jamie_Mitco_SPB_21.07468.07468.4 2.9066 0.2663 91.7 1980.3365 1980.9567 3557.0 309 4.467 29.8 1 G.T*RLSYFSSTTDT*RER.G YBR133C 2 4 4.5% 827 95153 6.4 U HSL7 SGDID:S000000337, Chr II from 504281-501798, reverse complement, Verified ORF, "Protein arginine N-methyltransferase that exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p" * Jamie_Mitco_SPB_58.20592.20592.2 3.2583 0.1851 100.0 2654.5923 2652.9177 8411.7 1 3.577 38.6 2 K.KLDNDDTPSYIGLLSSWLELESR.D * Jamie_Mitco_SPB_17.05844.05844.2 2.9298 0.2629 100.0 1705.7522 1703.8058 8955.4 1 5.147 61.5 2 R.HEDLEEDVPEVHVR.V YPL119C 1 1 4.5% 617 67917 8.9 U DBP1 SGDID:S000006040, Chr XVI from 326263-324410, reverse complement, Verified ORF, "Putative ATP-dependent RNA helicase of the DEAD-box protein family; mutants show reduced stability of the 40S ribosomal subunit scanning through 5' untranslated regions of mRNAs" * Jamie_Mitco_SPB_72.25654.25654.2 1.7763 0.2733 88.1 3050.412 3051.9956 9426.9 27 4.315 24.1 1 R.GGGGFFNSRNNGS*RDY*RKHGGNGSFGST.R Reverse_YOL115W 1 1 4.5% 584 66031 5.5 U PAP2 SGDID:S000005475, Chr XV from 101474-103228, Verified ORF, "Catalytic subunit of TRAMP (Trf4/Pap2p-Mtr4p-Air1p/2p), a nuclear poly (A) polymerase complex involved in RNA quality control; catalyzes polyadenylation of unmodified tRNAs, and snoRNA and rRNA precursors; disputed role as a DNA polymerase" * Jamie_Mitco_SPB_46.16516.16516.3 2.8122 0.0229 80.7 3182.3943 3182.5588 7176.3 21 3.225 22.0 1 R.S*PLIEVENEDNLS*ISKQPKRVKKIKK.S YKL060C 1 1 4.5% 359 39621 5.8 U FBA1 SGDID:S000001543, Chr XI from 327131-326052, reverse complement, Verified ORF, "Fructose 1,6-bisphosphate aldolase, a cytosolic enzyme required for glycolysis and gluconeogenesis; catalyzes the conversion of fructose 1,6 bisphosphate into two 3-carbon products: glyceraldehyde-3-phosphate and dihydroxyacetone phosphate" * Jamie_Mitco_SPB_28.09945.09945.2 3.3381 0.3928 100.0 1902.6721 1903.065 8676.6 1 6.815 50.0 1 K.VNLDTDCQYAYLTGIR.D Reverse_YOR206W 2 2 4.4% 710 81601 8.5 U NOC2 SGDID:S000005732, Chr XV from 727512-729644, Verified ORF, "Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors" * Jamie_Mitco_SPB_121.43339.43339.2 1.442 0.0448 84.3 1106.7522 1105.1497 7778.8 56 2.736 56.2 1 K.LSPS*DHLQK.K * Jamie_Mitco_SPB_37.13127.13127.2 1.5794 0.0633 87.3 2430.612 2432.653 6695.6 4 3.049 31.0 1 K.ASKKLQQQERTGAADAKEQDTK.N YDR194C 2 2 4.4% 664 76269 9.0 U MSS116 SGDID:S000002602, Chr IV from 847941-845947, reverse complement, Verified ORF, "DEAD-box protein required for efficient splicing of mitochondrial Group I and II introns; presumed RNA helicase due to DEAD-box motif" * Jamie_Mitco_SPB_37.13037.13037.2 3.0179 0.1701 91.8 1895.4122 1894.912 9218.9 1 4.025 50.0 1 D.DSEIS*FRGNKNYNNR.S * Jamie_Mitco_SPB_10.03424.03424.3 2.5839 0.0778 92.0 1819.8544 1822.716 5558.8 442 2.807 32.7 1 R.S*FSRS*NDKNNYSSR.N Reverse_YOR386W 1 1 4.4% 565 66274 9.1 U PHR1 SGDID:S000005913, Chr XV from 1066835-1068532, Verified ORF, "DNA photolyase involved in photoreactivation, repairs pyrimidine dimers in the presence of visible light; induced by DNA damage; regulated by transcriptional repressor Rph1p" * Jamie_Mitco_SPB_95.34199.34199.2 1.6106 0.0053 81.3 2923.9722 2925.4067 8616.5 3 3.665 22.9 1 K.AKANKQRLQQFLAVSKYLGVNDYLR.L Reverse_YDR507C 2 2 4.3% 1142 129858 9.2 U GIN4 SGDID:S000002915, Chr IV from 1465776-1462348, reverse complement, Verified ORF, "Protein kinase involved in bud growth and assembly of the septin ring, proposed to have kinase-dependent and kinase-independent activities; undergoes autophosphorylation; similar to Kcc4p and Hsl1p" * Jamie_Mitco_SPB_70.24955.24955.3 2.7495 0.0425 84.6 3131.5144 3131.5625 5408.1 11 2.896 24.1 1 K.RTFQPVRIESIKRLRGDSVPATTATSDK.L * Jamie_Mitco_SPB_83.29689.29689.2 1.5997 0.1124 91.7 2556.112 2556.8447 9663.6 9 3.395 30.0 1 K.NFS*IPKKRSSTVSILSERNRR.R Reverse_YLR035C 2 2 4.2% 695 78248 8.1 U MLH2 SGDID:S000004025, Chr XII from 214457-212370, reverse complement, Verified ORF, "Protein required for DNA mismatch repair in mitosis and meiosis; involved in the repair of mutational intermediates; forms a complex with Pms1p and Msh1p to repair mismatched DNA" * Jamie_Mitco_SPB_30.10590.10590.2 1.6738 0.0115 83.9 1680.1122 1678.6879 6971.7 269 2.965 37.5 1 K.S*NRPIT*MDVTNNR.K * Jamie_Mitco_SPB_18.06419.06419.2 2.9415 0.0535 99.0 1904.1122 1903.105 7146.0 1 2.978 46.7 1 K.YRKGNT*IGGKSDVLWK.E YJL128C 1 1 4.2% 668 72720 9.0 U PBS2 SGDID:S000003664, Chr X from 180021-178015, reverse complement, Verified ORF, "MAP kinase kinase that plays a pivotal role in the osmosensing signal-transduction pathway, activated under severe osmotic stress" * Jamie_Mitco_SPB_59.21175.21175.2 1.9275 0.2476 88.2 3096.2922 3098.2832 9806.2 9 4.344 22.2 1 T.NILCS*ANQGTVKLCDFGVSGNLVAS*LAK.T YDR120C 1 1 4.2% 570 64052 9.1 U TRM1 SGDID:S000002527, Chr IV from 693256-691544, reverse complement, Verified ORF, "tRNA methyltransferase, localizes to both the nucleus and mitochondrion to produce the modified base N2,N2-dimethylguanosine in tRNAs in both compartments" * Jamie_Mitco_SPB_64.22793.22793.2 1.4038 0.0552 82.7 2808.112 2807.0154 7636.4 84 3.489 23.9 1 R.EGRNNKT*FTKYSVAQGPPVDTKCK.F Reverse_YFR019W 4 4 4.1% 2278 257448 8.3 U FAB1 SGDID:S000001915, Chr VI from 184490-191326, Verified ORF, "1-phosphatidylinositol-3-phosphate 5-kinase; vacuolar membrane kinase that generates phosphatidylinositol (3,5)P2, which is involved in vacuolar sorting and homeostasis" * Jamie_Mitco_SPB_16.05545.05545.3 2.1395 0.0713 84.3 1668.8644 1667.872 5223.6 28 2.799 38.5 1 K.MLLSKEPQPTS*EVK.N * Jamie_Mitco_SPB_34.12217.12217.2 1.8265 0.0085 85.5 2181.0122 2181.1697 7733.2 122 2.624 30.6 1 K.SYENALSALGCLSS*DSS*MK.R * Jamie_Mitco_SPB_73.26165.26165.2 1.6304 0.0355 85.1 2717.5322 2716.7917 7969.8 5 3.124 30.0 1 K.EDHLS*KKDES*DKRSSDYFLKK.L * Jamie_Mitco_SPB_85.30349.30349.3 2.1425 0.0921 82.5 4586.4243 4582.087 12571.0 104 2.966 14.1 1 K.SPLAKSFVVGDIYESNQPS*GGSIRKIKVYTQRFDLTNLDR.A Reverse_YIL126W 2 2 4.1% 1359 156742 6.7 U STH1 SGDID:S000001388, Chr IX from 117992-122071, Verified ORF, "ATPase component of the ATP-dependent RSC chromatin remodeling complex required for kinetochore function in chromosome segregation; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p" * Jamie_Mitco_SPB_104.37228.37228.3 1.8714 0.1349 84.7 4771.6445 4769.3833 11864.2 101 3.372 15.8 1 K.EIVKFYDPYDRKSPLKEFISTRPHSDVEDLQERMEEVLK.E * Jamie_Mitco_SPB_10.03413.03413.3 2.2386 0.0902 87.0 2162.3044 2164.3003 6271.0 85 3.223 31.2 1 K.DEAT*VISITRKKRSS*RR.K Reverse_YBL105C 2 2 4.1% 1151 131519 7.1 U PKC1 SGDID:S000000201, Chr II from 17696-14241, reverse complement, Verified ORF, "Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)" * Jamie_Mitco_SPB_12.03981.03981.4 3.1063 0.0933 81.5 3125.2966 3127.1284 5309.9 37 3.273 22.7 1 K.EPSSFEAT*QQAGETFNLY*AENEDIFK.N * Jamie_Mitco_SPB_63.22463.22463.2 1.9243 0.2341 82.1 2672.392 2674.7515 7707.0 12 4.542 27.5 1 R.QDGDIQYLKT*LKT*NAEQYQKE.V YOR219C 2 2 4.1% 931 107200 6.0 U STE13 SGDID:S000005745, Chr XV from 755009-752214, reverse complement, Verified ORF, "Dipeptidyl aminopeptidase, Golgi integral membrane protein that cleaves on the carboxyl side of repeating -X-Ala- sequences, required for maturation of alpha factor, transcription is induced by a-factor" * Jamie_Mitco_SPB_75.26826.26826.2 1.8731 0.076 92.9 2296.9321 2298.4248 9738.5 28 3.038 29.4 1 R.FLYEGVEFTVS*TVQINY*K.L * Jamie_Mitco_SPB_35.12381.12381.3 2.5701 0.0513 84.9 2261.1843 2265.5278 6010.0 78 3.06 30.3 1 K.KY*PILVNIYGGPGSQTFTTK.S YBR203W 2 2 4.1% 924 104505 9.6 U COS111 SGDID:S000000407, Chr II from 629163-631937, Verified ORF, "Protein required for wild-type resistance to the antifungal drug ciclopirox olamine; not related to the COS family of subtelomerically-encoded proteins" * Jamie_Mitco_SPB_43.15385.15385.2 2.118 0.2343 89.6 2337.9922 2337.5088 7074.0 7 4.528 28.3 1 S.NGSGSNNAGKNSTTVGKLSSISQK.S * Jamie_Mitco_SPB_55.19467.19467.2 2.0216 0.0116 89.6 1723.5122 1724.744 8291.6 21 3.336 42.3 1 K.TS*HSGKHENSRFEK.L Reverse_YAR035W 2 2 4.1% 687 77766 6.9 U YAT1 SGDID:S000000080, Chr I from 190187-192250, Verified ORF, "Outer mitochondrial carnitine acetyltransferase, minor ethanol-inducible enzyme involved in transport of activated acyl groups from the cytoplasm into the mitochondrial matrix" * Jamie_Mitco_SPB_68.24172.24172.2 2.2649 0.2159 89.2 2155.872 2154.3093 6318.6 3 4.458 40.6 1 S.VDFYDYGSLLYKMDES*K.T * Jamie_Mitco_SPB_19.06563.06563.1 1.1075 0.0281 84.6 1253.15 1252.2834 4638.9 43 3.111 45.0 1 R.SSVVVSVS*HDR.I YMR283C 1 1 4.1% 513 58882 7.7 U RIT1 SGDID:S000004896, Chr XIII from 836866-835325, reverse complement, Verified ORF, "2'-O-ribosyl phosphate transferase, modifies the initiator methionine tRNA at position 64 to distinguish it from elongator methionine tRNA" * Jamie_Mitco_SPB_59.20921.20921.3 2.3259 0.0568 80.3 2233.3743 2230.6104 4279.8 2 2.924 31.2 1 K.ITNEISLGKVSSGLTINKNLK.Q Reverse_YDR376W 1 1 4.1% 493 56237 8.9 U ARH1 SGDID:S000002784, Chr IV from 1226812-1228293, Verified ORF, "Oxidoreductase of the mitochondrial inner membrane, involved in cytoplasmic and mitochondrial iron homeostasis and required for activity of Fe-S cluster-containing enzymes; one of the few mitochondrial proteins essential for viability" * Jamie_Mitco_SPB_86.30869.30869.2 1.7503 0.0222 85.7 2220.5122 2218.5269 8359.8 3 3.205 36.8 1 K.S*FEPMPVGAYGLSTILLDVK.Y Reverse_YOR313C 1 1 4.1% 338 38591 9.6 U SPS4 SGDID:S000005840, Chr XV from 902869-901853, reverse complement, Verified ORF, "Protein whose expression is induced during sporulation; not required for sporulation; heterologous expression in E. coli induces the SOS response that senses DNA damage" * Jamie_Mitco_SPB_10.03389.03389.2 1.6217 0.017 82.9 1645.8522 1646.9712 7422.5 3 2.733 50.0 1 R.PKT*QALIIRTLAIK.K YMR167W 2 2 4.0% 769 87062 7.0 U MLH1 SGDID:S000004777, Chr XIII from 594885-597194, Verified ORF, "Protein required for mismatch repair in mitosis and meiosis, postmeiotic segregation, and spore viability; forms a complex with Pms1p and Msh2p to repair mismatched DNA; human homolog is associated with hereditary non-polyposis colon cancer" * Jamie_Mitco_SPB_92.32901.32901.2 1.8138 0.0236 88.0 2423.872 2426.4758 6712.6 85 3.126 31.6 1 K.SLRQAQVVENS*YTT*ANSQLR.K * Jamie_Mitco_SPB_63.22417.22417.2 1.6097 0.0317 85.5 1450.1522 1448.5356 6613.2 11 3.144 50.0 1 K.LREKVDDS*IHR.E Reverse_YPL270W 2 2 4.0% 773 85137 9.1 U MDL2 SGDID:S000006191, Chr XVI from 30482-32803, Verified ORF, "Half-type ATP-binding cassette (ABC) transporter of the inner mitochondrial membrane" * Jamie_Mitco_SPB_7.02241.02241.2 1.4923 0.2053 98.2 1022.65216 1023.1771 3460.6 394 3.699 50.0 1 K.HGVTPSISPK.R * Jamie_Mitco_SPB_59.20904.20904.2 1.4952 0.1267 90.9 2511.4922 2511.8503 6179.4 14 3.585 27.5 1 R.LCLRSLPILQSSSPTVLS*RFR.I Reverse_contaminant_KERATIN02 1 1 4.0% 622 61987 5.2 U no description * Jamie_Mitco_SPB_21.07377.07377.4 3.178 0.2689 84.0 2422.3765 2419.4001 3980.3 243 4.436 23.6 1 R.SGRGY*SGGS*GGRGGLGIKGAGSSEF.D YJR049C 1 1 4.0% 530 59469 7.2 U UTR1 SGDID:S000003810, Chr X from 528390-526798, reverse complement, Verified ORF, "NAD kinase, active as a hexamer; enhances the activity of ferric reductase (Fre1p)" * Jamie_Mitco_SPB_60.21495.21495.2 1.882 0.1048 96.3 1979.9521 1979.0275 6626.2 20 3.788 30.0 1 K.DSSLVNGNANSGGGTSINGTR.G YFL022C 1 1 4.0% 503 57511 5.8 U FRS2 SGDID:S000001872, Chr VI from 95008-93497, reverse complement, Verified ORF, "Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar" * Jamie_Mitco_SPB_38.13463.13463.2 2.0799 0.0943 97.5 2325.4722 2324.5464 7712.8 16 3.406 34.2 1 R.DLQDTFYIKDPLTADLPDDK.T Reverse_YOR198C 1 1 4.0% 470 54639 9.2 U BFR1 SGDID:S000005724, Chr XV from 720065-718653, reverse complement, Verified ORF, "Component of mRNP complexes associated with polyribosomes; implicated in secretion and nuclear segregation; multicopy suppressor of BFA (Brefeldin A) sensitivity" * Jamie_Mitco_SPB_99.35519.35519.2 1.5922 0.0617 87.7 2334.7322 2335.7068 6818.7 32 3.02 27.8 1 R.T*KLDAQIKIIEKNKDQLKK.R YDR125C 1 1 4.0% 453 53185 9.5 U ECM18 SGDID:S000002532, Chr IV from 702753-701392, reverse complement, Verified ORF, "Protein of unknown function, similar to Rlp24p" * Jamie_Mitco_SPB_95.33929.33929.2 1.8163 0.0097 85.5 1943.9722 1942.286 5393.6 66 3.034 32.4 1 K.LGKMNVVGHSFGGYLSFK.Y YHR076W 1 1 4.0% 374 41191 6.4 U PTC7 SGDID:S000001118, Chr VIII from 251103-252227, Verified ORF, "Mitochondrially localized type 2C protein phosphatase; expression induced by growth on ethanol and by sustained osmotic stress; possible role in carbon source utilization in low oxygen environments" * Jamie_Mitco_SPB_31.11016.11016.2 2.3898 0.192 87.7 1907.8922 1908.2076 3137.0 4 4.131 46.4 1 A.FQPKDRDDLIYQKLK.D YER166W 3 3 3.9% 1571 177797 6.0 U DNF1 SGDID:S000000968, Chr V from 512739-517454, Verified ORF, "Non-essential P-type ATPase that is a potential aminophospholipid translocase, localizes to the plasma membrane and late exocytic or early endocytic membranes, likely involved in protein transport" * Jamie_Mitco_SPB_46.16281.16281.4 2.7451 0.1394 84.6 4027.5764 4030.4443 7830.7 278 3.39 16.2 1 K.GVKVGDIVRIHNNDEIPADIILLSTSDTDGACYVETK.N * Jamie_Mitco_SPB_48.17193.17193.2 1.2877 0.083 82.6 3056.412 3054.3228 7598.4 90 3.175 21.7 1 R.TKFWIESEGPHS*NLYTYQGNMKWR.N * Jamie_Mitco_SPB_53.18762.18762.2 1.6335 0.1245 93.4 2826.7122 2825.0435 7853.0 15 3.807 26.2 1 K.FWIES*EGPHSNLYTYQGNMKWR.N Reverse_YLL021W 2 2 3.9% 1466 163142 5.2 U SPA2 SGDID:S000003944, Chr XII from 100946-105346, Verified ORF, "Component of the polarisome, which functions in actin cytoskeletal organization during polarized growth; acts as a scaffold for Mkk1p and Mpk1p cell wall integrity signaling components; potential Cdc28p substrate" * Jamie_Mitco_SPB_29.10150.10150.3 2.3323 0.0631 83.7 2195.4543 2195.4749 6239.3 190 2.659 29.2 1 K.LKSNLYNIEDKLSAEEVTK.V * Jamie_Mitco_SPB_88.31462.31462.4 2.9563 0.0976 80.3 4019.9365 4025.5461 7720.8 255 2.911 17.1 1 K.IPALPSNKALEPSVIQSNPEPLGSKKLTAS*SPTKEVSK.P Reverse_YBL007C 3 3 3.9% 1244 135848 5.9 U SLA1 SGDID:S000000103, Chr II from 216369-212635, reverse complement, Verified ORF, "Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; contains 3 SH3 domains; interacts with proteins regulating actin dynamics and with proteins required for endocytosis" * Jamie_Mitco_SPB_34.12093.12093.2 1.462 0.0134 80.7 1359.4321 1359.6757 5646.6 6 2.847 55.0 1 R.GFKKDLHKMVR.V * Jamie_Mitco_SPB_74.26566.26566.2 1.6418 0.0032 82.2 1928.9521 1929.7361 5517.6 27 3.155 31.2 1 R.ES*DRSDT*GRSSSGDNAK.F * Jamie_Mitco_SPB_59.21137.21137.3 2.8547 0.0857 92.8 2610.5942 2607.7996 5555.8 41 3.54 27.5 1 R.DVWLRS*KKPNPFDKTAS*SNKK.H contaminant_UBIQUITIN01 2 2 3.9% 1089 121884 7.5 U no description * Jamie_Mitco_SPB_93.33197.33197.2 1.4439 0.0569 83.3 1610.6721 1611.8008 5671.1 55 3.315 34.6 1 R.QHTPLPLGSADYRR.V * Jamie_Mitco_SPB_74.26602.26602.4 3.3326 0.1026 84.7 3489.5764 3490.842 5566.9 172 3.453 21.4 1 K.GHMAKCY*GDLVQELWSGTQKS*VAPLKLRR.T YDR385W 2 2 3.9% 842 93289 6.3 U EFT2 SGDID:S000002793, Chr IV from 1243220-1245748, Verified ORF, "Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin" YOR133W 2 2 3.9% 842 93289 6.3 U EFT1 SGDID:S000005659, Chr XV from 575098-577626, Verified ORF, "Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin" Jamie_Mitco_SPB_25.08650.08650.2 3.3962 0.2095 100.0 2147.652 2146.371 9264.8 1 4.659 52.8 1 K.STAISLYSEMSDEDVKEIK.Q Jamie_Mitco_SPB_10.03508.03508.2 3.3295 0.1828 100.0 1493.3121 1492.644 4099.6 26 5.326 46.2 1 K.TGTLTTSETAHNMK.V YKR099W 2 2 3.9% 811 89604 8.2 U BAS1 SGDID:S000001807, Chr XI from 635489-637924, Verified ORF, "Myb-related transcription factor involved in regulating basal and induced expression of genes of the purine and histidine biosynthesis pathways" * Jamie_Mitco_SPB_50.17717.17717.2 1.4336 0.0781 85.5 1710.5721 1711.9281 5460.2 56 3.668 39.3 1 R.S*KVSLPIEGLSQMNK.Q * Jamie_Mitco_SPB_55.19537.19537.2 1.8489 0.0739 92.3 1994.0322 1991.8937 6108.3 327 3.078 25.0 1 R.KSESSQHHSSS*SVT*TNK.F Reverse_YNR015W 1 1 3.9% 384 42816 8.8 U SMM1 SGDID:S000005298, Chr XIV from 653389-654543, Verified ORF, "Dihydrouridine synthase, member of a family of dihydrouridine synthases including Dus1p, Smm1p, Dus3p, and Dus4p; modifies uridine residues at position 20 of cytoplasmic tRNAs" * Jamie_Mitco_SPB_65.23093.23093.2 1.7224 0.0754 90.4 1850.5721 1852.9775 9127.3 9 3.3 42.9 1 K.RCHVTLNTIGT*ACLR.K Reverse_YNL003C 1 1 3.9% 284 31027 9.6 U PET8 SGDID:S000004948, Chr XIV from 625831-624977, reverse complement, Verified ORF, "S-adenosylmethionine transporter of the mitochondrial inner membrane, member of the mitochondrial carrier family; required for biotin biosynthesis and respiratory growth" * Jamie_Mitco_SPB_40.14375.14375.2 1.4073 0.0188 80.5 1243.1721 1244.3494 5501.6 25 2.777 50.0 1 K.WPEVQSQGNAK.A YOR330C 1 1 3.8% 1254 143502 8.8 U MIP1 SGDID:S000005857, Chr XV from 943380-939616, reverse complement, Verified ORF, "Catalytic subunit of the mitochondrial DNA polymerase" * Jamie_Mitco_SPB_81.29136.29136.4 2.819 0.1685 90.7 5799.4165 5805.375 8219.1 73 3.988 12.4 1 K.TLIT*DPISFPLPPLQGRS*LDEHFQKIGRFNSEPYKSFCEDKFTEMVAR.P YDR040C 2 2 3.8% 1091 120357 5.6 U ENA1 SGDID:S000002447, Chr IV from 538463-535188, reverse complement, Verified ORF, "P-type ATPase sodium pump, involved in Na+ and Li+ efflux to allow salt tolerance" * Jamie_Mitco_SPB_54.19440.19440.2 2.0285 0.0502 92.9 2814.3323 2816.015 7703.6 124 2.885 22.0 1 R.KLDSLEALGAVNDICSDKTGTLT*QGK.M * Jamie_Mitco_SPB_18.06475.06475.2 2.1615 0.1979 81.6 1681.9521 1681.7972 6779.6 14 3.61 46.4 1 Y.STSTT*IHTEVNIGIK.Q Reverse_YNL212W 1 1 3.8% 782 88846 5.1 U VID27 SGDID:S000005156, Chr XIV from 247462-249810, Verified ORF, "Cytoplasmic protein of unknown function; possibly involved in vacuolar protein degradation; not essential for proteasome-dependent degradation of fructose-1,6-bisphosphatase (FBPase); null mutants exhibit normal growth" * Jamie_Mitco_SPB_66.23735.23735.4 3.5239 0.2441 80.0 3589.8164 3593.0312 5465.2 115 4.063 21.3 1 R.IPKKTYT*LISASHEPRIKLVYTKVNES*APF.S YML031W 1 1 3.8% 655 74134 8.3 U NDC1 SGDID:S000004493, Chr XIII from 214189-216156, Verified ORF, "Nuclear envelope protein with multiple putative transmembrane domains, required for nuclear pore complex assembly and spindle pole body duplication; required for meiosis II" * Jamie_Mitco_SPB_53.18778.18778.2 4.8611 0.494 100.0 2896.7722 2896.1233 6653.4 1 8.666 45.8 1 R.SICALGEFADWDPESMAYTAFQTQR.T YGR278W 2 2 3.8% 577 67294 6.9 U CWC22 SGDID:S000003510, Chr VII from 1046737-1048470, Verified ORF, "Essential protein, component of a complex containing Cef1p, has similarity to S. pombe Cwf22p; putative spliceosomal component, based on computational analysis of large-scale protein-protein interaction data" * Jamie_Mitco_SPB_17.05809.05809.2 1.9505 0.0055 87.8 1901.9122 1902.1149 8437.4 254 2.881 42.9 1 K.LRYILQTQELSST*LR.E * Jamie_Mitco_SPB_2.00639.00639.1 1.1481 0.1243 97.4 876.51 877.1582 4104.5 5 3.849 66.7 1 K.KIYLVLK.S Reverse_YPL112C 1 1 3.8% 394 44911 9.0 U PEX25 SGDID:S000006033, Chr XVI from 338619-337435, reverse complement, Verified ORF, "Peripheral peroxisomal membrane peroxin required for the regulation of peroxisome size and maintenance, recruits GTPase Rho1p to peroxisomes, induced by oleate, interacts with homologous protein Pex27p" * Jamie_Mitco_SPB_92.32823.32823.2 1.6512 0.0432 86.6 1577.3922 1578.5028 6291.0 71 3.058 39.3 1 R.SSETHSESGSGAS*FK.E Reverse_YKL114C 1 1 3.8% 367 41439 8.2 U APN1 SGDID:S000001597, Chr XI from 224099-222996, reverse complement, Verified ORF, "Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; controls spontaneous mutations" * Jamie_Mitco_SPB_60.21324.21324.2 1.7282 0.1191 95.9 1938.2522 1939.9469 7591.5 12 3.502 42.3 1 K.DIEEQT*YQPS*VWKR.P Reverse_YGR098C 2 2 3.7% 1630 187446 8.2 U ESP1 SGDID:S000003330, Chr VII from 687462-682570, reverse complement, Verified ORF, "Separase with cysteine protease activity (related to caspases) that promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibited by Pds1p" * Jamie_Mitco_SPB_51.18021.18021.3 2.6159 0.0592 84.8 3912.7744 3911.6296 7744.4 1 3.307 20.5 1 R.ASGIRIHIKILQFFSFSLSKLLALRS*GQSLKNKK.K * Jamie_Mitco_SPB_109.39157.39157.3 2.5122 0.0494 81.0 3216.6543 3220.4778 4410.7 19 2.891 24.0 1 K.EFKLITRHSTKSDNSILVNS*IELNAT*K.L YGL131C 3 3 3.7% 1403 163203 8.6 U SNT2 SGDID:S000003099, Chr VII from 265864-261653, reverse complement, Verified ORF, "DNA binding protein with similarity to the S. pombe Snt2 protein" * Jamie_Mitco_SPB_98.35215.35215.3 2.477 0.0367 80.3 2615.3943 2618.909 5415.5 110 2.97 29.2 1 R.RFRY*IYT*EPKYPLENVLKK.Y * Jamie_Mitco_SPB_19.06659.06659.2 2.1115 0.0386 95.9 1229.8121 1229.2432 6415.5 65 3.252 60.0 1 R.VTGGS*DDEKIK.I * Jamie_Mitco_SPB_34.12024.12024.2 1.4931 0.0803 87.3 2549.0923 2547.719 7533.5 21 2.903 28.6 1 K.Y*GNGQSMQPALNTTAVLIKHS*R.F YGR094W 2 2 3.7% 1104 125770 7.0 U VAS1 SGDID:S000003326, Chr VII from 672190-675504, Verified ORF, "Mitochondrial and cytoplasmic valyl-tRNA synthetase" * Jamie_Mitco_SPB_48.17235.17235.2 1.3615 0.0698 83.3 1148.1122 1148.2615 6217.0 11 2.976 50.0 1 K.KNAAATTGASQK.K * Jamie_Mitco_SPB_102.36573.36573.3 2.6242 0.0878 90.0 3398.2444 3394.631 5428.0 68 2.85 21.4 1 K.LNT*AISNLEVENKDVKSRTLLSVPGY*DEK.V Reverse_YIL156W 2 2 3.7% 1071 123134 7.9 U UBP7 SGDID:S000001418, Chr IX from 48091-51306, Verified ORF, "Ubiquitin-specific protease that cleaves ubiquitin-protein fusions" * Jamie_Mitco_SPB_28.09799.09799.2 2.3461 0.1883 87.0 1505.3922 1505.7117 6103.5 9 3.737 54.2 1 D.ELLVS*PRVPVSTK.R * Jamie_Mitco_SPB_64.22799.22799.3 2.6001 0.1358 93.0 3209.8442 3210.517 6187.6 35 3.528 21.2 1 R.RLLENSYEPTFPT*IHPHMATGKDDDLM.- Q0050 2 2 3.7% 834 96079 9.9 U AI1 SGDID:S000007261, Chr Mito from 13818-16322, Verified ORF, "Reverse transcriptase required for splicing of the COX1 pre-mRNA, encoded by a mobile group II intron within the mitochondrial COX1 gene" * Jamie_Mitco_SPB_26.09226.09226.2 1.5069 0.119 92.0 1359.5322 1361.7498 5832.1 34 2.802 50.0 1 K.FKPMRMVNIPK.P * Jamie_Mitco_SPB_96.34339.34339.2 1.6177 0.022 83.1 2639.8323 2641.9495 8084.0 195 2.794 23.7 1 R.ERIYYVLYYSCVLTLAS*KYR.L YOR361C 1 1 3.7% 763 88130 6.0 U PRT1 SGDID:S000005888, Chr XV from 1017648-1015357, reverse complement, Verified ORF, "Subunit of the core complex of translation initiation factor 3(eIF3), essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes" * Jamie_Mitco_SPB_83.29718.29718.2 3.7899 0.5256 100.0 3241.7122 3242.4683 9771.8 1 10.14 35.2 1 K.IFDVQPEDASDDFTTIEEIVEEVLEETK.E Reverse_contaminant_PIGR_HUMAN 1 1 3.7% 764 83314 5.7 U owl|P01833| POLYMERIC-IMMUNOGLOBULIN RECEPTOR PRECURSOR (PLGR) (CONTAINS:... * Jamie_Mitco_SPB_76.27262.27262.4 3.1012 0.1652 93.8 3222.9766 3221.443 6637.7 332 3.585 22.8 1 R.GEFAPARKGLT*NVVVDCNEGSSQRCLFK.A Reverse_contaminant_GR78_MOUSE 1 1 3.7% 655 72421 5.2 U owl|P20029| 78 KD GLUCOSE REGULATED PROTEIN PRECURSOR (GRP 78) (IMMUNOGLOBULIN... * Jamie_Mitco_SPB_55.19564.19564.3 2.9085 0.1574 96.1 2832.5344 2831.964 6418.2 90 3.491 25.0 1 R.EGEY*VKITVTPQNDSATSFIQS*KK.T YDL025C 1 1 3.7% 620 69615 8.3 U YDL025C SGDID:S000002183, Chr IV from 407203-405341, reverse complement, Uncharacterized ORF, "Protein of unknown function, potentially phosphorylated by Cdc28p" * Jamie_Mitco_SPB_117.42031.42031.2 1.2731 0.0741 81.0 2448.5122 2448.6152 7367.5 232 3.597 25.0 1 K.LGEGASGSVS*VVERTDGKLFACK.M YPR156C 1 1 3.7% 622 68066 7.1 U TPO3 SGDID:S000006360, Chr XVI from 839773-837905, reverse complement, Verified ORF, "Polyamine transport protein specific for spermine; localizes to the plasma membrane; member of the major facilitator superfamily" * Jamie_Mitco_SPB_47.16872.16872.2 1.8749 0.1339 97.4 2342.132 2343.614 8416.8 251 3.383 22.7 1 K.SLQDMGVSSKAPVPDVNAPQSSK.N Reverse_YER014W 1 1 3.7% 539 59703 9.3 U HEM14 SGDID:S000000816, Chr V from 182599-184218, Verified ORF, "Protoporphyrinogen oxidase, a mitochondrial enzyme that catalyzes the seventh step in the heme biosynthetic pathway, converting protoporphyrinogen IX to protoporphyrin IX" * Jamie_Mitco_SPB_21.07275.07275.3 2.8874 0.0271 85.9 2191.2544 2189.4802 6576.7 1 3.337 38.2 1 K.KDAICGKDISQIVAEKGLDK.L YFL021W 2 2 3.7% 510 56328 8.6 U GAT1 SGDID:S000001873, Chr VI from 95964-97496, Verified ORF, "Transcriptional activator of genes involved in nitrogen catabolite repression, member of the GATA family of DNA binding proteins; activity and localization regulated by nitrogen limitation and Ure2p" Jamie_Mitco_SPB_1.00349.00349.1 1.7944 0.0449 97.3 812.56 813.07446 4462.5 1 3.336 75.0 1 K.KKPALKK.I * Jamie_Mitco_SPB_53.18850.18850.2 1.5999 0.0743 89.4 1297.3322 1296.4691 4255.8 75 4.07 45.5 1 K.KNYTASVAASKR.K YPL101W 1 1 3.7% 456 51156 9.5 U ELP4 SGDID:S000006022, Chr XVI from 360206-361576, Verified ORF, "Elongator protein, part of the HAP subcomplex of Elongator, which is a six-subunit component of the RNA polymerase II holoenzyme; required for Elongator structural integrity and histone acetyltransferase activity" * Jamie_Mitco_SPB_98.35016.35016.2 1.7211 0.0301 86.5 1986.7122 1987.3988 6064.6 115 3.23 28.1 1 K.IQHGLVHILKLPVFTDR.G YBR236C 1 1 3.7% 436 50341 8.0 U ABD1 SGDID:S000000440, Chr II from 691688-690378, reverse complement, Verified ORF, "Methyltransferase, catalyzes the transfer of a methyl group from S-adenosylmethionine to the GpppN terminus of capped mRNA" * Jamie_Mitco_SPB_18.06241.06241.2 1.6159 0.0249 83.4 1846.7122 1847.8009 7401.7 105 2.877 30.0 1 R.QYGS*ITGDESST*VSKK.D YHR118C 1 1 3.7% 435 50296 7.9 U ORC6 SGDID:S000001160, Chr VIII from 345631-344324, reverse complement, Verified ORF, "Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; may be phosphorylated by Cdc28p" * Jamie_Mitco_SPB_87.31295.31295.2 1.813 0.1412 97.5 1734.6322 1733.9248 6549.7 53 3.729 40.0 1 K.RSPVKNGGRFTSSDPK.E Reverse_YHR080C 2 2 3.6% 1345 149679 8.7 U YHR080C SGDID:S000001122, Chr VIII from 266840-262803, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Mitco_SPB_23.08044.08044.4 3.0639 0.0994 82.4 4278.5366 4283.555 5889.6 72 3.797 17.6 1 K.HEDDNRLMARGRPSIASGRNS*IWDWIDSERGVIPS*K.D * Jamie_Mitco_SPB_44.15640.15640.2 1.5548 0.0624 87.5 1413.3522 1413.6526 4840.3 18 3.403 45.8 1 K.MGMSISGPSFSRR.N YBR202W 2 2 3.6% 845 94875 5.3 U CDC47 SGDID:S000000406, Chr II from 625767-628304, Verified ORF, "Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase" * Jamie_Mitco_SPB_16.05562.05562.2 1.5444 0.0943 89.6 1340.0122 1338.3893 5894.7 13 3.047 50.0 1 K.GS*S*GVGLTAAVMK.D * Jamie_Mitco_SPB_105.37476.37476.2 2.0946 0.2078 81.4 1879.5521 1880.3713 5358.8 266 4.078 34.4 1 R.TLLGIIRLSQALAKLRL.A YPL075W 2 2 3.6% 785 87729 8.3 U GCR1 SGDID:S000005996, Chr XVI from 412251-412258,413010-415359, Verified ORF, "Transcriptional activator of genes involved in glycolysis; DNA-binding protein that interacts and functions with the transcriptional activator Gcr2p" * Jamie_Mitco_SPB_77.27631.27631.2 1.9362 0.0076 87.8 2337.7922 2335.505 8427.6 138 3.282 26.5 1 K.VDSYFMELS*KKLTT*ENIR.L * Jamie_Mitco_SPB_51.18195.18195.2 1.3933 0.1096 89.1 1193.3322 1190.4282 4330.8 152 3.591 44.4 1 K.ILVGDLEKFR.I YBR156C 1 1 3.6% 698 79186 9.9 U SLI15 SGDID:S000000360, Chr II from 553194-551098, reverse complement, Verified ORF, "Subunit of the Ipl1p-Sli15p-Bir1p complex that regulates kinetochore-microtubule attachments, activation of the spindle tension checkpoint, and mitotic spindle disassembly; regulates the activity and localization of the Ipl1p aurora kinase" * Jamie_Mitco_SPB_65.23207.23207.3 3.5799 0.1184 96.5 3019.9744 3021.1838 5177.7 319 3.979 20.8 1 K.ISVDSSSPSKEVKNY*Y*QSPVRGYLR.P YDR320C 1 1 3.6% 668 75020 5.3 U SWA2 SGDID:S000002728, Chr IV from 1108096-1106090, reverse complement, Verified ORF, "Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles" * Jamie_Mitco_SPB_63.22365.22365.3 2.6196 0.0622 86.8 2658.7744 2658.8394 6207.7 206 3.214 23.9 1 K.KQNLTASSSNSPISVDSTS*EIKKR.E Reverse_YER025W 1 1 3.6% 527 57866 6.8 U GCD11 SGDID:S000000827, Chr V from 205250-206833, Verified ORF, "Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met" * Jamie_Mitco_SPB_20.06958.06958.3 2.8096 0.0064 80.4 2309.6343 2309.3901 7483.3 166 2.649 30.6 1 K.ENIETCAPS*T*LQLRAMDAK.V YLR423C 1 1 3.6% 417 48656 5.1 U ATG17 SGDID:S000004415, Chr XII from 973166-971913, reverse complement, Verified ORF, "Protein that interacts with and is required for activation of Apg1p protein kinase; involved in autophagy but not in the Cvt (cytoplasm to vacuole targeting) pathway" * Jamie_Mitco_SPB_106.37999.37999.2 1.7963 0.0986 93.8 1952.5122 1952.983 5988.0 4 3.774 42.9 1 K.IDDFSSLYT*LNY*NVK.N YBL034C 2 2 3.5% 1513 174176 6.3 U STU1 SGDID:S000000130, Chr II from 158392-153851, reverse complement, Verified ORF, "Component of the mitotic spindle that binds to interpolar microtubules via its association with beta-tubulin (Tub2p); required for interpolar microtubules to provide an outward force on the spindle poles" * Jamie_Mitco_SPB_21.07479.07479.4 3.5937 0.1484 94.4 3811.2566 3807.017 6558.0 28 4.093 19.1 1 R.HHDSMNSVS*NSNTKDNNNVTKRKVSAPPSSTAATK.V * Jamie_Mitco_SPB_63.22441.22441.3 2.7537 0.2628 84.0 2130.9543 2131.4326 3976.4 35 4.492 32.4 1 F.NHMSKAINRIKSGSFT*MK.H YGR238C 1 1 3.5% 882 99975 8.1 U KEL2 SGDID:S000003470, Chr VII from 968692-966044, reverse complement, Verified ORF, "Protein that functions in a complex with Kel1p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate" * Jamie_Mitco_SPB_60.21469.21469.3 2.612 0.2056 98.1 3468.4744 3464.7766 6065.5 41 4.048 20.8 1 K.NFPVLRGLTVDSEEYGSSSYKDTSCQKGIPK.N YOR112W 1 1 3.5% 761 84769 5.4 U YOR112W SGDID:S000005638, Chr XV from 531508-533793, Uncharacterized ORF, "Hypothetical protein" * Jamie_Mitco_SPB_86.30651.30651.3 2.6566 0.04 82.1 2992.8542 2994.2026 5256.0 364 2.863 22.1 1 K.TTILAPRNIASNSTVT*TKSSLSNKT*AR.S YKL054C 2 2 3.5% 738 83973 5.0 U DEF1 SGDID:S000001537, Chr XI from 338397-336181, reverse complement, Verified ORF, "RNAPII degradation factor, forms a complex with Rad26p in chromatin, enables ubiquitination and proteolysis of RNAPII" * Jamie_Mitco_SPB_30.10513.10513.3 3.7641 0.3392 100.0 3093.8643 3093.427 7786.0 1 5.389 28.0 1 A.HFYQQSFPYGQPQYGVAGQYPYQLPK.N * Jamie_Mitco_SPB_29.10397.10397.2 2.1595 0.2455 91.8 2389.5522 2389.672 7210.1 2 4.646 37.5 1 Q.SFPYGQPQYGVAGQYPYQLPK.N YFR051C 1 1 3.5% 546 60628 5.2 U RET2 SGDID:S000001947, Chr VI from 251790-250150, reverse complement, Verified ORF, "Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER" * Jamie_Mitco_SPB_41.14608.14608.2 1.4935 0.0409 82.6 2215.4922 2213.3225 7914.9 92 2.91 30.6 1 K.EVINAEFSRDGT*IHSSELK.G Reverse_YMR190C 2 2 3.4% 1447 163837 6.3 U SGS1 SGDID:S000004802, Chr XIII from 645257-640914, reverse complement, Verified ORF, "Nucleolar DNA helicase of the RecQ family involved in maintenance of genome integrity, regulates chromosome synapsis and meiotic crossing over; has similarity to human BLM and WRN helicases implicated in Bloom and Werner syndromes" * Jamie_Mitco_SPB_5.01726.01726.2 1.6444 0.0596 88.4 1795.3322 1797.0238 5054.6 160 2.934 30.8 1 K.SLDAIT*AKFYKFRR.R * Jamie_Mitco_SPB_98.35129.35129.4 2.8919 0.1352 84.7 4057.7766 4060.2422 5143.5 302 3.258 17.6 1 K.SS*LNSKSVIDSLSFDDDFDDEEDKQSVSSKERKIK.P YKR001C 1 1 3.4% 704 78736 7.9 U VPS1 SGDID:S000001709, Chr XI from 442365-440251, reverse complement, Verified ORF, "GTPase required for vacuolar protein sorting, functions in actin cytoskeleton organization via its interaction with Sla1p; required for late Golgi-retention of some proteins including Kex2p; involved in regulating peroxisome biogenesis" * Jamie_Mitco_SPB_53.18749.18749.2 1.6848 0.0967 91.8 2760.5122 2762.3647 7562.8 276 3.161 21.7 1 R.T*IADIIPKALMLKLIVKSKTDIQK.V YLL043W 1 1 3.4% 669 73877 6.9 U FPS1 SGDID:S000003966, Chr XII from 49937-51946, Verified ORF, "Plasma membrane glycerol channel, member of the major intrinsic protein (MIP) family of channel proteins; involved in efflux of glycerol and in uptake of the trivalent metalloids arsenite and antimonite" * Jamie_Mitco_SPB_76.27030.27030.2 1.5612 0.0937 89.6 2701.2722 2698.8328 7433.8 86 3.342 22.7 1 R.MALQKT*KTKS*SISDNENEAGEKK.V YNL039W 1 1 3.4% 594 67688 6.9 U BDP1 SGDID:S000004984, Chr XIV from 555049-556833, Verified ORF, "Essential subunit of RNA polymerase III transcription factor (TFIIIB), which is involved in transcription of genes encoding tRNAs, 5S rRNA, U6 snRNA, and other small RNAs" * Jamie_Mitco_SPB_73.26050.26050.2 2.158 0.2781 80.0 2045.5721 2045.436 5752.4 95 4.74 26.3 1 S.SAKKPAGAHRISIVSKISPP.T YGL202W 1 1 3.4% 500 56178 6.0 U ARO8 SGDID:S000003170, Chr VII from 116063-117565, Verified ORF, "Aromatic aminotransferase, expression is regulated by general control of amino acid biosynthesis" * Jamie_Mitco_SPB_71.25390.25390.2 2.0919 0.1249 98.4 1893.0922 1893.1503 6309.8 294 4.312 37.5 1 K.SLANTFLSLDTEGRVIR.M YBR143C 1 1 3.4% 437 49006 5.0 U SUP45 SGDID:S000000347, Chr II from 532176-530863, reverse complement, Verified ORF, "Polypeptide release factor involved in translation termination; mutant form acts as a recessive omnipotent suppressor" * Jamie_Mitco_SPB_98.35225.35225.2 1.4606 0.1415 91.9 1699.1322 1697.9268 5927.7 2 3.063 39.3 1 R.LS*VLSAITSTQQKLK.L YPR163C 1 1 3.4% 436 48522 5.3 U TIF3 SGDID:S000006367, Chr XVI from 869951-868641, reverse complement, Verified ORF, "Translation initiation factor eIF-4B, has RNA annealing activity; contains an RNA recognition motif and binds to single-stranded RNA" * Jamie_Mitco_SPB_21.07386.07386.2 2.203 0.0816 97.5 1842.1921 1841.8978 6623.9 42 3.838 42.9 1 R.GS*NFQSSSRPPRRER.E Reverse_YPL084W 2 2 3.3% 844 97276 5.4 U BRO1 SGDID:S000006005, Chr XVI from 394035-396569, Verified ORF, "Cytoplasmic class E vacuolar protein sorting (VPS) factor that coordinates deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes" * Jamie_Mitco_SPB_70.25071.25071.2 1.7198 0.0071 83.4 2209.392 2212.3093 7918.9 22 2.696 33.3 1 R.MLQGQTDTNSFINGDS*FQK.E * Jamie_Mitco_SPB_13.04333.04333.1 2.0149 0.0054 95.3 1162.84 1163.3159 5085.8 6 3.19 62.5 1 K.KWDLKETDK.L YLL008W 2 2 3.3% 752 84843 5.7 U DRS1 SGDID:S000003931, Chr XII from 131728-133986, Verified ORF, "Nucleolar DEAD-box protein required for ribosome assembly and function, including synthesis of 60S ribosomal subunits; constituent of 66S pre-ribosomal particles" * Jamie_Mitco_SPB_33.11754.11754.2 2.4101 0.195 89.7 1956.3722 1955.2223 6396.0 23 3.395 40.0 1 D.DEKVEAKKT*TKKRKGK.N * Jamie_Mitco_SPB_35.12402.12402.2 1.3784 0.0259 80.8 1157.4521 1155.2993 3871.7 1 2.984 62.5 1 R.SYRKTKTDR.I Reverse_YOR354C 1 1 3.3% 692 79947 8.1 U MSC6 SGDID:S000005881, Chr XV from 1003220-1001142, reverse complement, Verified ORF, "Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to mitochondria; msc6 mutants are defective in directing meiotic recombination events to homologous chromatids" * Jamie_Mitco_SPB_62.22036.22036.3 2.6565 0.0406 83.5 2924.9343 2924.107 6210.3 160 3.138 23.9 1 R.ISSDKALNY*YKEIYPVYES*HKSK.Y Reverse_YDR320C 1 1 3.3% 668 75020 5.3 U SWA2 SGDID:S000002728, Chr IV from 1108096-1106090, reverse complement, Verified ORF, "Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles" * Jamie_Mitco_SPB_80.28660.28660.3 2.2128 0.0675 80.3 2451.1743 2454.4778 6312.5 154 2.894 25.0 1 K.KIESTSDVSIPS*NSSS*ATLNQK.K YHR091C 1 1 3.3% 643 73694 9.4 U MSR1 SGDID:S000001133, Chr VIII from 286772-284841, reverse complement, Verified ORF, "Mitochondrial arginyl-tRNA synthetase" * Jamie_Mitco_SPB_55.19607.19607.2 1.9826 0.0674 93.5 2594.612 2594.7515 9528.7 32 3.115 27.5 1 K.TLNTKY*TDQPEGPIY*PLDVLR.L YKL148C 1 1 3.3% 640 70229 7.5 U SDH1 SGDID:S000001631, Chr XI from 171134-169212, reverse complement, Verified ORF, "Flavoprotein subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone" * Jamie_Mitco_SPB_31.11137.11137.2 1.3526 0.0834 84.2 2587.652 2587.6826 8587.4 198 3.269 22.5 1 R.VIDHT*LDEKECPSVPPT*VRAY.- Reverse_YKL012W 1 1 3.3% 583 69065 8.9 U PRP40 SGDID:S000001495, Chr XI from 417953-419704, Verified ORF, "U1 snRNP protein involved in splicing, interacts with the branchpoint-binding protein during the formation of the second commitment complex" * Jamie_Mitco_SPB_54.19158.19158.3 2.396 0.1431 93.4 2447.1843 2451.5806 7378.6 10 3.397 29.2 1 R.S*FSWT*SDVQNEKLMTIFEK.E YDR402C 1 1 3.3% 489 56071 8.7 U DIT2 SGDID:S000002810, Chr IV from 1273693-1272224, reverse complement, Verified ORF, "N-formyltyrosine oxidase, sporulation-specific microsomal enzyme required for spore wall maturation, involved in the production of a soluble LL-dityrosine-containing precursor of the spore wall, homologous to cytochrome P-450s" * Jamie_Mitco_SPB_35.12478.12478.2 1.7406 0.0571 89.4 1806.7722 1808.0166 7166.6 77 3.185 33.3 1 R.LLEGQTS*IPMGPLSQR.M YOR216C 1 1 3.3% 484 56069 4.9 U RUD3 SGDID:S000005742, Chr XV from 748979-747525, reverse complement, Verified ORF, "Golgi matrix protein involved in the structural organization of the cis-Golgi; interacts genetically with COG3 and USO1" * Jamie_Mitco_SPB_33.11879.11879.3 2.2486 0.0762 85.6 2088.1743 2092.2761 5268.5 10 3.286 36.7 1 K.HDIIEKHS*KELNTYRK.E Reverse_YIL096C 1 1 3.3% 336 39402 9.7 U YIL096C SGDID:S000001358, Chr IX from 183124-182114, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Mitco_SPB_121.43397.43397.3 2.2043 0.0958 90.5 1458.1144 1462.5625 6140.5 17 3.668 42.5 1 R.LEHKQKS*EINR.V Reverse_YPL217C 1 1 3.2% 1183 135571 6.8 U BMS1 SGDID:S000006138, Chr XVI from 143170-139619, reverse complement, Verified ORF, "Essential conserved nucleolar GTP-binding protein required for synthesis of 40S ribosomal subunits and for processing of the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, has similarity to Tsr1p" * Jamie_Mitco_SPB_105.37799.37799.3 2.0687 0.097 81.9 4231.3145 4235.7676 10767.7 14 3.961 18.2 1 R.EQGPVFS*PEENKKGIDIYVADKDMLVGGVDSMPAYILK.D Reverse_YCL061C 1 1 3.2% 1096 124326 5.3 U MRC1 SGDID:S000000566, Chr III from 22106-18816, reverse complement, Verified ORF, "S-phase checkpoint protein found at replication forks, required for DNA replication; also required for Rad53p activation during DNA replication stress, where it forms a replication-pausing complex with Tof1p and is phosphorylated by Mec1p; protein involved in replication checkpoint" * Jamie_Mitco_SPB_51.18298.18298.4 3.4553 0.128 89.1 4089.3364 4087.19 9579.2 16 3.341 18.6 1 K.ELEEGGDYLNS*ILQTPLS*NESRDKGNNHNQEPAKK.G Reverse_YKR086W 2 2 3.2% 1071 121652 8.2 U PRP16 SGDID:S000001794, Chr XI from 599499-602714, Verified ORF, "RNA helicase in the DEAH-box family involved in the second catalytic step of splicing, exhibits ATP-dependent RNA unwinding activity" * Jamie_Mitco_SPB_50.17751.17751.2 1.7934 0.0474 89.2 2202.652 2205.3765 7338.6 2 3.315 35.3 1 K.NSNDTMKLEQIVS*DRMDK.C * Jamie_Mitco_SPB_103.36953.36953.2 1.4481 0.0107 80.0 2057.2922 2056.2488 5458.5 144 2.913 30.0 1 K.PNYVKLKS*YGCDIVYR.I YDR074W 2 2 3.2% 896 102976 8.1 U TPS2 SGDID:S000002481, Chr IV from 593889-596579, Verified ORF, "Phosphatase subunit of the trehalose-6-phosphate synthase/phosphatase complex, which synthesizes the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway" * Jamie_Mitco_SPB_52.18478.18478.2 1.9857 0.1452 98.9 1571.9321 1571.6842 6727.9 24 3.604 45.5 1 K.EEKSNLES*KLWK.E * Jamie_Mitco_SPB_120.43199.43199.2 1.6527 0.086 90.4 2120.7522 2118.1072 9510.1 71 3.715 34.4 1 K.FLSSLKEQAS*S*NDDMER.K YML029W 1 1 3.2% 838 96653 5.1 U USA1 SGDID:S000004491, Chr XIII from 217362-219878, Verified ORF, "Protein that interacts in the two-hybrid system with the U1 snRNP-specific protein, Snp1p; may have a role in pre-mRNA splicing" * Jamie_Mitco_SPB_31.11088.11088.3 2.7754 0.1489 94.9 3088.1943 3087.0764 7206.5 105 3.412 21.2 1 R.AADNTSSANDNNTVENDES*AWNRRVVR.P Reverse_YBR202W 1 1 3.2% 845 94875 5.3 U CDC47 SGDID:S000000406, Chr II from 625767-628304, Verified ORF, "Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase" * Jamie_Mitco_SPB_69.24669.24669.3 2.7223 0.0541 86.5 3030.5645 3028.4734 7493.4 313 2.757 22.1 1 R.KDVGGVLLLLLAKKVDLNGY*IEPAIS*K.A Reverse_YPL105C 1 1 3.2% 849 94357 7.8 U YPL105C SGDID:S000006026, Chr XVI from 355409-352860, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Mitco_SPB_85.30347.30347.3 3.2294 0.0792 92.8 3102.1443 3102.3826 8075.4 300 4.027 21.2 1 K.EKKSSNEKKLDELASSIQNY*VAGEPKK.N Reverse_YBL008W 1 1 3.2% 840 93890 7.2 U HIR1 SGDID:S000000104, Chr II from 209656-212178, Verified ORF, "Non-essential transcriptional corepressor involved in the cell cycle-regulated transcription of histone H2A, H2B, H3 and H4 genes; contributes to nucleosome formation, heterochromatic gene silencing, and formation of functional kinetochores" * Jamie_Mitco_SPB_43.15207.15207.3 2.7085 0.1024 92.6 2977.8843 2980.2156 5385.2 8 3.534 24.0 1 K.SDLTPKKIGNSFPS*SSSTSILT*PAVRK.K YOR354C 2 2 3.2% 692 79947 8.1 U MSC6 SGDID:S000005881, Chr XV from 1003220-1001142, reverse complement, Verified ORF, "Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to mitochondria; msc6 mutants are defective in directing meiotic recombination events to homologous chromatids" * Jamie_Mitco_SPB_24.08355.08355.2 1.6062 0.076 90.8 1131.5721 1129.2609 5646.1 5 3.72 62.5 1 R.EKHVSPFER.V * Jamie_Mitco_SPB_37.12965.12965.2 1.8339 0.0294 88.9 1689.9722 1692.8444 6663.0 22 3.021 45.8 1 R.RMLSTEFS*SLERR.D YLR035C 2 2 3.2% 695 78248 8.1 U MLH2 SGDID:S000004025, Chr XII from 214457-212370, reverse complement, Verified ORF, "Protein required for DNA mismatch repair in mitosis and meiosis; involved in the repair of mutational intermediates; forms a complex with Pms1p and Msh1p to repair mismatched DNA" * Jamie_Mitco_SPB_92.32903.32903.2 1.7231 0.0079 84.9 1513.3722 1510.6971 4318.0 117 3.081 41.7 1 K.VSCPVGT*TVILRK.L * Jamie_Mitco_SPB_14.04981.04981.2 1.5365 0.0111 82.9 1119.0721 1119.2933 6641.9 410 2.737 62.5 1 K.KASCMHKTR.P YIL014W 1 1 3.2% 630 72410 5.6 U MNT3 SGDID:S000001276, Chr IX from 326101-327993, Verified ORF, "Alpha-1,3-mannosyltransferase, adds the fourth and fifth alpha-1,3-linked mannose residues to O-linked glycans during protein O-glycosylation" * Jamie_Mitco_SPB_42.15082.15082.2 1.5929 0.0152 82.7 2472.4321 2474.5225 7135.9 12 2.51 26.3 1 K.TCKISNSAEDDFGREPEY*FK.S YIL036W 1 1 3.2% 587 65264 8.0 U CST6 SGDID:S000001298, Chr IX from 285665-287428, Verified ORF, "Basic leucine zipper (bZIP) transcription factor of the ATF/CREB family, activates transcription of genes involved in utilization of non-optimal carbon sources" * Jamie_Mitco_SPB_88.31653.31653.3 2.7503 0.0846 92.9 2363.6042 2362.4324 6766.5 5 3.354 33.3 1 K.QKDNNKRGIDDT*S*KILNNK.I Reverse_YGL064C 1 1 3.2% 561 63058 9.9 U MRH4 SGDID:S000003032, Chr VII from 383211-381526, reverse complement, Verified ORF, "Mitochondrial RNA helicase, plays an essential role in mitochondrial function" * Jamie_Mitco_SPB_123.43948.43948.2 1.2995 0.1079 85.4 2041.7122 2039.4258 7201.3 283 4.172 26.5 1 K.VADRVPPLILLQDFNSIK.Q Reverse_YJL008C 1 1 3.2% 568 61662 5.7 U CCT8 SGDID:S000003545, Chr X from 421578-419872, reverse complement, Verified ORF, "Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo" * Jamie_Mitco_SPB_59.20922.20922.2 1.7899 0.0188 86.7 1886.1921 1883.2389 7344.4 160 3.021 29.4 1 K.TIRSILEIETAGAGPLLK.G YDR195W 1 1 3.2% 533 59819 9.6 U REF2 SGDID:S000002603, Chr IV from 848594-850195, Verified ORF, "RNA-binding protein involved in the cleavage step of mRNA 3'-end formation prior to polyadenylation, and in snoRNA maturation; part of holo-CPF subcomplex APT, which associates with 3'-ends of snoRNA- and mRNA-encoding genes" * Jamie_Mitco_SPB_105.37785.37785.2 1.8377 0.2856 90.6 2050.2922 2053.1965 6235.3 97 4.019 34.4 1 Q.LVNIS*HALQAST*IQQIR.L YFR010W 1 1 3.2% 499 57111 7.2 U UBP6 SGDID:S000001906, Chr VI from 165060-166559, Verified ORF, "Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; deletion causes hypersensitivity to cycloheximide and other toxic compounds" * Jamie_Mitco_SPB_99.35619.35619.2 1.5493 0.0378 83.4 1757.1921 1755.0393 5151.5 3 3.833 46.7 1 R.QKYMVKGGLSGEESIK.I Reverse_contaminant_KERATIN12 1 1 3.2% 431 47974 5.0 U no description * Jamie_Mitco_SPB_79.28432.28432.2 1.5956 0.0485 86.4 1833.0322 1833.8662 6709.7 12 3.058 38.5 1 K.KY*QT*LHADEGELLR.R YPR116W 1 1 3.2% 277 31133 9.2 U YPR116W SGDID:S000006320, Chr XVI from 758646-759479, Uncharacterized ORF, "Hypothetical protein" * Jamie_Mitco_SPB_15.05082.05082.2 1.5288 0.0351 85.5 1195.9922 1196.3123 4413.8 5 3.192 68.8 1 K.KLLIDCPT*R.V YPL058C 3 3 3.1% 1511 171064 6.8 U PDR12 SGDID:S000005979, Chr XVI from 450374-445839, reverse complement, Verified ORF, "Plasma membrane weak-acid-inducible ATP-binding cassette (ABC) transporter, required for weak organic acid resistance, strongly induced by sorbate and benzoate, regulated by War1p, mutants exhibit sorbate hypersensitivity" Jamie_Mitco_SPB_8.02541.02541.1 1.0758 0.1083 95.5 870.52 869.86676 2868.8 26 3.407 57.1 1 R.GVS*GGERK.R * Jamie_Mitco_SPB_47.16601.16601.2 2.5621 0.299 100.0 2525.3123 2525.644 8595.0 213 5.428 26.3 1 K.SSSEFEEYWLNSEDYQELLR.T * Jamie_Mitco_SPB_62.22313.22313.2 1.8773 0.0496 90.5 2340.9722 2338.4092 11406.9 221 3.201 25.0 1 K.ERHEKDTNIFQT*VPGDENK.I YMR205C 1 1 3.1% 959 104618 6.7 U PFK2 SGDID:S000004818, Chr XIII from 674765-671886, reverse complement, Verified ORF, "Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes" * Jamie_Mitco_SPB_67.24063.24063.3 2.7299 0.1829 96.9 3351.3245 3349.5671 7645.1 20 4.137 21.6 1 K.PTPPPAPEASAESGLSS*KVHS*YTDLAYRMK.T YJL141C 1 1 3.1% 807 91245 8.4 U YAK1 SGDID:S000003677, Chr X from 150308-147885, reverse complement, Verified ORF, "Serine-threonine protein kinase that is part of a glucose-sensing system involved in growth control in response to glucose availability; translocates from the cytoplasm to the nucleus and phosphorylates Pop2p in response to a glucose signal" * Jamie_Mitco_SPB_96.34371.34371.2 1.521 0.1742 96.4 2986.0322 2986.3928 9178.1 50 4.5 22.9 1 K.VVKSRTEYLTQS*ITEAKILELLNQK.I Reverse_YPL075W 1 1 3.1% 785 87729 8.3 U GCR1 SGDID:S000005996, Chr XVI from 412251-412258,413010-415359, Verified ORF, "Transcriptional activator of genes involved in glycolysis; DNA-binding protein that interacts and functions with the transcriptional activator Gcr2p" * Jamie_Mitco_SPB_86.30677.30677.2 1.3645 0.1314 89.3 3063.5923 3061.362 10348.4 130 3.431 21.7 1 K.EEETMSRVNWNKFNEFICIEKNNK.T Reverse_YDR221W 1 1 3.1% 702 79908 4.9 U YDR221W SGDID:S000002629, Chr IV from 907324-909432, Uncharacterized ORF, "Hypothetical protein" * Jamie_Mitco_SPB_81.28975.28975.2 1.5828 0.0315 83.3 2564.2522 2562.0684 6991.4 10 3.417 26.2 1 K.ELSVLDKRSSFINLKSLIEKLK.N YLR153C 2 2 3.1% 683 75492 6.7 U ACS2 SGDID:S000004143, Chr XII from 447576-445525, reverse complement, Verified ORF, "Acetyl-coA synthetase isoform, required for growth on glucose; expressed under anaerobic conditions" * Jamie_Mitco_SPB_54.19281.19281.2 1.9258 0.1941 99.8 1345.5922 1347.5596 3969.4 83 4.137 54.5 1 K.ATHFYVAPTALR.L * Jamie_Mitco_SPB_19.06496.06496.2 1.7968 0.2056 99.8 1226.4321 1225.3054 4350.6 1 4.823 81.2 1 R.DHDGYYWIR.G Reverse_YKL198C 1 1 3.1% 649 72062 8.9 U PTK1 SGDID:S000001681, Chr XI from 70223-68274, reverse complement, Verified ORF, "Putative serine/threonine protein kinase that regulates spermine uptake; involved in polyamine transport; possible mitochondrial protein" * Jamie_Mitco_SPB_84.30121.30121.2 1.7575 0.0074 84.6 2204.112 2204.2778 7935.0 76 2.7 28.9 1 K.TSASSLSRKSNTTHAFS*NAR.R contaminant_INT-STD1 3 3 3.1% 607 69271 6.1 U BSA * Jamie_Mitco_SPB_21.07295.07295.2 2.8832 0.3664 100.0 1421.5322 1420.5713 5247.6 1 6.096 72.7 1 K.SLHTLFGDELCK.V * Jamie_Mitco_SPB_21.07369.07369.3 3.7812 0.3108 100.0 1422.7144 1420.5713 4444.9 1 6.095 47.7 1 K.SLHTLFGDELCK.V * Jamie_Mitco_SPB_13.04521.04521.1 2.085 0.0114 97.3 759.18 758.9024 3959.8 57 2.606 66.7 1 K.GACLLPK.I Reverse_contaminant_INT-STD1 1 1 3.1% 607 69270 6.1 U BSA * Jamie_Mitco_SPB_87.31324.31324.2 1.5285 0.0359 82.9 2238.8523 2237.4907 6926.2 50 3.403 30.6 1 R.RNVLSETCCKTVKESVPTK.E YDR515W 2 2 3.1% 447 50943 9.3 U SLF1 SGDID:S000002923, Chr IV from 1473419-1474762, Verified ORF, "RNA binding protein that associates with polysomes; proposed to be involved in regulating mRNA translation; involved in the copper-dependent mineralization of copper sulfide complexes on cell surface in cells cultured in copper salts" * Jamie_Mitco_SPB_25.08929.08929.3 2.2703 0.043 80.2 1768.5844 1773.0208 5165.7 198 2.826 38.5 1 K.NSKNS*KTNKKMKKR.G * Jamie_Mitco_SPB_15.05337.05337.2 1.5029 0.0049 82.7 1032.1522 1034.3085 6141.8 296 3.046 64.3 1 K.TNKKMKKR.G Reverse_YLL045C 1 1 3.1% 256 28112 10.0 U RPL8B SGDID:S000003968, Chr XII from 48628-47858, reverse complement, Verified ORF, "Ribosomal protein L4 of the large (60S) ribosomal subunit, nearly identical to Rpl8Ap and has similarity to rat L7a ribosomal protein; mutation results in decreased amounts of free 60S subunits" * Jamie_Mitco_SPB_5.01723.01723.1 1.2295 0.0398 91.5 865.34 863.98956 4697.1 3 3.11 64.3 1 K.AKNSKTSK.A YFL036W 2 2 3.0% 1351 153047 8.9 U RPO41 SGDID:S000001858, Chr VI from 58781-62836, Verified ORF, "Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition" * Jamie_Mitco_SPB_93.33437.33437.2 1.6853 0.0307 85.6 1661.2522 1663.0312 4971.1 11 3.331 39.3 1 K.PIISAYLKMSVNGIR.E * Jamie_Mitco_SPB_15.05173.05173.3 2.7334 0.1894 97.3 2895.8643 2896.0134 5683.3 80 4.033 21.9 1 K.DRVAFTESHLQDIKDSAENPLT*GDR.W Reverse_YDL019C 2 2 3.0% 1283 145795 6.8 U OSH2 SGDID:S000002177, Chr IV from 421511-417660, reverse complement, Verified ORF, "Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability" * Jamie_Mitco_SPB_13.04564.04564.3 2.6684 0.0425 83.0 2804.5745 2805.028 5301.5 345 3.028 19.4 1 K.TGDDTPGNGASAATRTKELSLDGDKVVK.K * Jamie_Mitco_SPB_17.05886.05886.2 1.7265 0.0309 88.3 1343.3722 1343.3904 6184.8 27 2.76 50.0 1 K.QST*KEVNSEIK.S Reverse_YKR090W 1 1 3.0% 706 79448 5.2 U PXL1 SGDID:S000001798, Chr XI from 608588-610708, Verified ORF, "LIM domain-containing protein that localizes to sites of polarized growth, required for selection and/or maintenance of polarized growth sites, may modulate signaling by the GTPases Cdc42p and Rho1p; has similarity to metazoan paxillin" * Jamie_Mitco_SPB_59.21138.21138.2 1.9188 0.0529 91.3 2423.4521 2425.5884 6297.7 62 3.002 25.0 1 R.FTEQSGMGENNSYEVNSQKFK.Y YCR048W 1 1 3.0% 610 71613 8.3 U ARE1 SGDID:S000000644, Chr III from 211924-213756, Verified ORF, "Acyl-CoA:sterol acyltransferase, isozyme of Are2p; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the absence of oxygen" * Jamie_Mitco_SPB_29.10277.10277.2 2.1323 0.2134 85.6 1997.4521 1998.9171 6132.6 167 4.773 29.4 1 R.SSTTS*LVEPVESTEGVES*.T YOR370C 1 1 3.0% 603 67374 4.7 U MRS6 SGDID:S000005897, Chr XV from 1030990-1029179, reverse complement, Verified ORF, "Rab escort protein, forms a complex with the Ras-like small GTPase Ypt1p that is required for the prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p)" * Jamie_Mitco_SPB_110.39247.39247.2 1.6412 0.0721 89.1 1940.5322 1941.1913 4331.5 105 3.058 29.4 1 K.LVSFNPTTKVATFQDGSK.V YDR117C 1 1 3.0% 565 63992 8.9 U YDR117C SGDID:S000002524, Chr IV from 685638-683941, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Mitco_SPB_98.35021.35021.2 1.7922 0.0405 88.6 1861.0521 1862.0441 5999.8 36 2.872 34.4 1 K.GDDLTIVGKNTDKDELK.N YBR057C 1 1 3.0% 366 41433 5.9 U MUM2 SGDID:S000000261, Chr II from 353291-352191, reverse complement, Verified ORF, "Cytoplasmic protein essential for meiotic DNA replication and sporulation; interacts with Orc2p, which is a component of the origin recognition complex" * Jamie_Mitco_SPB_4.01308.01308.2 3.2099 0.0659 88.0 1359.9922 1359.4789 8207.9 2 4.124 80.0 1 S.QIESLENEIQR.L Reverse_YHR023W 2 2 2.9% 1928 223634 6.4 U MYO1 SGDID:S000001065, Chr VIII from 151659-157445, Verified ORF, "Type II myosin heavy chain, required for wild-type cytokinesis and cell separation; localizes to the actomyosin ring; binds to myosin light chains Mlc1p and Mlc2p through its IQ1 and IQ2 motifs respectively" * Jamie_Mitco_SPB_99.35316.35316.3 1.9644 0.2294 94.8 4774.824 4779.2266 8576.6 228 3.754 14.7 1 R.DLQES*LLAEKSKSQRLDLT*LQSLKGNSENLIEQLKYNELK.Y * Jamie_Mitco_SPB_40.14356.14356.2 1.927 0.0101 87.8 1926.4122 1926.1826 8631.0 243 2.937 33.3 1 R.SRKFKSSNQDWTSILK.S Reverse_YPL226W 2 2 2.9% 1196 134330 5.9 U NEW1 SGDID:S000006147, Chr XVI from 121767-125357, Verified ORF, "ATP binding cassette family member; Asn/Gln-rich rich region supports [NU+] prion formation, susceptibility to [PSI+] prion induction and aggregation of a fragment of the human Machado-Joseph Disease protein" * Jamie_Mitco_SPB_28.09773.09773.2 1.7315 0.0753 90.7 2462.632 2463.648 5837.6 47 3.604 26.3 1 R.GDDIDIEKTMMEKEDES*IKR.S * Jamie_Mitco_SPB_31.11105.11105.2 1.8975 0.0027 87.0 1718.4321 1715.9896 9576.2 13 2.773 42.9 1 K.QEVFAALNGKYYALK.K Reverse_YGL083W 2 2 2.9% 804 91001 8.1 U SCY1 SGDID:S000003051, Chr VII from 353061-355475, Verified ORF, "Putative kinase, suppressor of GTPase mutant, similar to bovine rhodopsin kinase" * Jamie_Mitco_SPB_21.07341.07341.2 1.4876 0.0276 81.7 1543.9722 1543.5164 4194.0 13 3.18 38.5 1 K.TSS*ADNSGGKT*MVK.S * Jamie_Mitco_SPB_3.01000.01000.1 1.109 0.0894 94.8 934.02 933.8614 4531.0 92 3.242 37.5 1 K.SPSSSSS*SK.P Reverse_YCL030C 1 1 2.9% 799 87721 5.3 U HIS4 SGDID:S000000535, Chr III from 68333-65934, reverse complement, Verified ORF, "Multifunctional enzyme containing phosphoribosyl-ATP pyrophosphatase, phosphoribosyl-AMP cyclohydrolase, and histidinol dehydrogenase activities; catalyzes the second, third, ninth and tenth steps in histidine biosynthesis" * Jamie_Mitco_SPB_95.34078.34078.2 1.4603 0.0155 80.4 2738.5522 2738.893 8947.1 6 2.92 27.3 1 K.QGNGS*TEGKIWIENRSRSYYVGR.G Reverse_YLR399C 1 1 2.9% 686 76978 6.0 U BDF1 SGDID:S000004391, Chr XII from 921596-919536, reverse complement, Verified ORF, "Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p" * Jamie_Mitco_SPB_46.16505.16505.3 2.1676 0.1063 87.6 2275.4944 2271.4614 5112.3 32 3.05 28.9 1 K.KDRRGKSSANKS*GSRGKSRK.G YCR088W 1 1 2.9% 592 65576 4.7 U ABP1 SGDID:S000000684, Chr III from 265064-266842, Verified ORF, "Actin-binding protein of the cortical actin cytoskeleton, important for activation of the Arp2/3 complex that plays a key role actin in cytoskeleton organization" * Jamie_Mitco_SPB_59.20920.20920.2 1.5363 0.0794 87.9 1791.0322 1788.0598 7948.9 236 3.54 34.4 1 R.SSAAPPPPPRRATPEKK.P Reverse_YPL228W 1 1 2.9% 549 61850 5.6 U CET1 SGDID:S000006149, Chr XVI from 118382-120031, Verified ORF, "Beta (RNA 5'-triphosphatase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CEG1, a guanylyltransferase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide" * Jamie_Mitco_SPB_44.15659.15659.2 1.6864 0.0299 85.5 1788.2922 1789.8973 7126.0 6 3.165 40.0 1 K.VVS*GVANGAIKKET*QK.Q YHR017W 1 1 2.9% 385 44235 8.9 U YSC83 SGDID:S000001059, Chr VIII from 138687-139844, Verified ORF, "Non-essential mitochondrial protein of unknown function; mRNA induced during meiosis, peaking between mid to late prophase of meiosis I; similar to S. douglasii YSD83" * Jamie_Mitco_SPB_38.13481.13481.1 1.4155 0.2196 85.7 1431.39 1432.5796 5194.6 49 4.318 35.0 1 D.S*NRSKVYKTLR.I YPL004C 1 1 2.9% 341 38071 4.7 U LSP1 SGDID:S000005925, Chr XVI from 551654-550629, reverse complement, Verified ORF, "Long chain base-responsive inhibitor of protein kinases Pkh1p and Pkh2p, acts along with Pil1p to down-regulate heat stress resistance via regulation of the Pkc1p and Ypk1p pathways; phosphorylated by Phk1p and Phk2p" * Jamie_Mitco_SPB_11.03857.03857.2 1.6884 0.099 94.2 1100.7522 1101.2046 6612.3 16 3.983 61.1 1 R.NIEASVQPSR.D YHR106W 1 1 2.9% 342 37087 6.9 U TRR2 SGDID:S000001148, Chr VIII from 325601-326629, Verified ORF, "Mitochondrial thioredoxin reductase involved in protection against oxidative stress, required with Glr1p to maintain the redox state of Trx3p, contains active-site motif (CAVC) present in prokaryotic thioredoxin reductases, binds NADPH and FAD" * Jamie_Mitco_SPB_39.13894.13894.2 1.7049 0.0249 87.7 1293.4922 1292.4777 5319.7 62 3.009 50.0 1 K.Y*ASKVYILVR.K YDR141C 2 2 2.8% 1698 194686 5.7 U DOP1 SGDID:S000002548, Chr IV from 739992-734896, reverse complement, Verified ORF, "Protein of unknown function, essential for viability, involved in establishing cellular polarity and morphogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern" * Jamie_Mitco_SPB_82.29299.29299.3 2.4191 0.171 93.8 4446.3843 4444.041 10536.6 274 4.256 15.5 1 R.LFLLEKREGGY*YQPIALLLGGLEELLEISHGYLVTEER.E * Jamie_Mitco_SPB_14.04731.04731.2 1.6325 0.092 93.0 1007.97217 1010.138 7076.5 5 3.652 68.8 1 R.TASVKFSNR.D YFL008W 3 3 2.8% 1225 141280 6.5 U SMC1 SGDID:S000001886, Chr VI from 119424-123101, Verified ORF, "Subunit of the multiprotein cohesin complex, essential protein involved in chromosome segregation and in double-strand DNA break repair; SMC chromosomal ATPase family member, binds DNA with a preference for DNA with secondary structure" * Jamie_Mitco_SPB_12.04162.04162.1 1.4552 0.0312 94.0 1310.01 1310.4069 3725.9 5 3.072 50.0 1 R.IES*LQKDLQR.Q * Jamie_Mitco_SPB_65.23104.23104.2 1.4284 0.0236 81.0 1200.5922 1202.3501 3130.8 41 2.555 50.0 1 K.Y*GLAVSTILGK.N * Jamie_Mitco_SPB_9.03241.03241.2 3.2518 0.1708 97.2 1474.0322 1476.546 4637.2 2 5.285 70.8 1 I.DELSNGQRSNSIR.A YDR122W 2 2 2.8% 1064 120071 8.7 U KIN1 SGDID:S000002529, Chr IV from 694695-697889, Verified ORF, "Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin2p" * Jamie_Mitco_SPB_12.04095.04095.2 1.5337 0.0517 85.4 1425.1721 1426.5438 3185.8 57 3.499 45.8 1 R.APAGS*MPSIDFPR.S Jamie_Mitco_SPB_24.08389.08389.2 1.753 0.0342 87.9 1866.6921 1865.1826 5266.2 51 2.889 37.5 1 K.GFFSVQTTSSKPLPIVR.Y Reverse_YMR089C 2 2 2.8% 825 93276 7.6 U YTA12 SGDID:S000004695, Chr XIII from 448085-445608, reverse complement, Verified ORF, "Component, with Afg3p, of the mitochondrial inner membrane m-AAA protease that mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes" * Jamie_Mitco_SPB_24.08587.08587.2 1.8421 0.0945 94.2 1817.5922 1817.1132 5897.2 173 3.513 32.1 1 R.EPFPRKGLLDIMDER.T * Jamie_Mitco_SPB_8.02878.02878.1 1.059 0.0259 82.5 891.55 891.0301 3752.8 1 2.722 64.3 1 K.GNDNLMVK.V YDL239C 1 1 2.8% 790 91740 8.6 U ADY3 SGDID:S000002398, Chr IV from 28775-26403, reverse complement, Verified ORF, "Protein required for spore wall formation, thought to mediate assembly of a Don1p-containing structure at the leading edge of the prospore membrane via interaction with spindle pole body components; potentially phosphorylated by Cdc28p" * Jamie_Mitco_SPB_83.29787.29787.3 2.5778 0.0676 86.4 2744.8145 2744.1348 5784.2 430 3.086 23.8 1 K.EFHNERKAASKRLNIVQDRFRK.E Reverse_YFL009W 1 1 2.8% 779 86090 7.1 U CDC4 SGDID:S000001885, Chr VI from 116139-118478, Verified ORF, "F-box protein required for G1/S and G2/M transition, associates with Skp1p and Cdc53p to form a complex, SCFCdc4, which acts as ubiquitin-protein ligase directing ubiquitination of the phosphorylated CDK inhibitor Sic1p" * Jamie_Mitco_SPB_37.13162.13162.2 1.9988 0.0489 92.2 2078.7322 2080.2192 7707.9 364 3.243 28.6 1 R.HTGAATVLATVS*GSSAIGGSVR.Y YPL270W 1 1 2.8% 773 85137 9.1 U MDL2 SGDID:S000006191, Chr XVI from 30482-32803, Verified ORF, "Half-type ATP-binding cassette (ABC) transporter of the inner mitochondrial membrane" * Jamie_Mitco_SPB_44.15541.15541.2 1.5953 0.0446 85.5 2634.9321 2637.6963 8375.2 18 2.944 26.2 1 K.GS*ARS*AHAKEQSKTDDYKDIIR.L Reverse_YMR179W 1 1 2.8% 758 84697 6.0 U SPT21 SGDID:S000004791, Chr XIII from 619857-622133, Verified ORF, "Protein required for normal transcription at several loci including HTA2-HTB2 and HHF2-HHT2, but not required at the other histone loci; functionally related to Spt10p" * Jamie_Mitco_SPB_29.10175.10175.3 2.5487 0.082 89.5 2381.9343 2380.4072 7758.1 216 3.458 25.0 1 R.GVTGS*NT*NNITITTRANRSNK.I Reverse_YPR083W 1 1 2.8% 579 64959 5.5 U MDM36 SGDID:S000006287, Chr XVI from 704852-706591, Verified ORF, "Protein required for normal mitochondrial morphology and inheritance" * Jamie_Mitco_SPB_37.13294.13294.2 1.7401 0.0068 84.1 1857.1322 1855.997 6330.5 6 2.794 40.0 1 R.IQDIDRSLSELALSS*K.E Reverse_YMR170C 1 1 2.8% 506 55188 5.6 U ALD2 SGDID:S000004780, Chr XIII from 603081-601561, reverse complement, Verified ORF, "Cytoplasmic aldehyde dehydrogenase, involved in ethanol oxidation and beta-alanine biosynthesis; uses NAD+ as the preferred coenzyme; expression is stress induced and glucose repressed; very similar to Ald3p" * Jamie_Mitco_SPB_65.23401.23401.2 1.5411 0.0453 84.7 1372.5721 1369.6475 3835.3 294 2.834 42.3 1 K.IIVTNGAALAGQLK.W YBR275C 3 3 2.7% 1916 217959 6.6 U RIF1 SGDID:S000000479, Chr II from 757101-751351, reverse complement, Verified ORF, "Protein that binds to the Rap1p C-terminus and acts synergistically with Rif2p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation" * Jamie_Mitco_SPB_29.10357.10357.2 2.1064 0.0274 91.7 2375.152 2375.5112 5655.9 5 3.561 30.0 1 R.IESSPIY*RIPGSSPKPS*PSSK.P * Jamie_Mitco_SPB_44.15580.15580.2 1.8931 0.0337 89.7 1925.4722 1926.1766 7050.1 24 3.013 34.4 1 K.FLSKLPLNSYDSANLDK.V * Jamie_Mitco_SPB_13.04455.04455.3 1.9343 0.1187 88.0 1552.5844 1552.6847 5902.1 154 3.238 37.5 1 K.NQEGNKS*LLSLLR.T YGL173C 2 2 2.7% 1528 175459 7.5 U KEM1 SGDID:S000003141, Chr VII from 180119-175533, reverse complement, Verified ORF, "5'-3' exonuclease involved in mRNA decay, evolutionarily conserved component of cytoplasmic processing (P) bodies, plays a role in microtubule-mediated processes, filamentous growth, and ribosomal RNA maturation" * Jamie_Mitco_SPB_27.09683.09683.2 2.4811 0.1913 100.0 2183.392 2183.265 7083.3 1 4.275 41.2 1 K.MDLDSINPDETEEEFQNR.V * Jamie_Mitco_SPB_99.35301.35301.2 1.4664 0.1379 91.7 2738.872 2739.0671 8307.8 131 3.613 21.7 1 K.ALEKKKQSNNRNNNTKTAHKTPSK.Q YLR189C 2 2 2.7% 1198 136054 7.5 U ATG26 SGDID:S000004179, Chr XII from 534395-530799, reverse complement, Verified ORF, "UDP-glucose:sterol glucosyltransferase, conserved enzyme involved in synthesis of sterol glucoside membrane lipids, involved in autophagy" * Jamie_Mitco_SPB_111.39637.39637.1 1.0581 0.121 96.4 1675.58 1676.8455 8179.0 78 3.224 33.3 1 K.MS*GNLNIRT*KLIR.S * Jamie_Mitco_SPB_40.14297.14297.2 1.9022 0.0109 87.8 2166.9922 2166.4387 6959.5 7 2.82 33.3 1 K.STFKPPAELQEFISEARSK.G YLR249W 2 2 2.7% 1044 115945 6.0 U YEF3 SGDID:S000004239, Chr XII from 636782-639916, Verified ORF, "Translational elongation factor, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing EF-1 alpha from the ribosomal complex; contains two ABC cassettes; binds and hydrolyses ATP" Jamie_Mitco_SPB_11.03666.03666.2 2.1704 0.141 99.4 1391.4722 1391.4778 3716.8 1 4.729 59.1 1 K.ATETVDNKDIER.F * Jamie_Mitco_SPB_16.05566.05566.2 2.4633 0.1758 87.8 1823.2322 1821.9073 5934.2 4 4.115 43.3 1 A.IANGQVDGFPTQEECR.T YNL014W 2 2 2.7% 1044 115869 6.2 U HEF3 SGDID:S000004959, Chr XIV from 606320-609454, Verified ORF, "Translational elongation factor EF-3; paralog of YEF3 and member of the ABC superfamily; stimulates EF-1 alpha-dependent binding of aminoacyl-tRNA by the ribosome; normally expressed in zinc deficient cells" Jamie_Mitco_SPB_11.03666.03666.2 2.1704 0.141 99.4 1391.4722 1391.4778 3716.8 1 4.729 59.1 1 K.ATETVDNKDIER.F * Jamie_Mitco_SPB_16.05418.05418.2 2.1674 0.1219 98.7 1747.7122 1747.0482 4752.8 318 3.778 33.3 1 K.INSGKKKSKLSSAELR.K Reverse_YDR028C 1 1 2.7% 1014 112616 5.1 U REG1 SGDID:S000002435, Chr IV from 500875-497831, reverse complement, Verified ORF, "Regulatory subunit of type 1 protein phosphatase Glc7p, involved in negative regulation of glucose-repressible genes" * Jamie_Mitco_SPB_44.15539.15539.3 2.5695 0.0377 80.1 3448.6143 3446.8352 6864.7 3 3.09 23.1 1 K.METMHKRAENLKWLANDEIIET*VT*RKK.L Reverse_YKL176C 1 1 2.7% 828 93359 8.7 U LST4 SGDID:S000001659, Chr XI from 117991-115505, reverse complement, Verified ORF, "Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface" * Jamie_Mitco_SPB_97.34865.34865.2 2.2144 0.1901 80.6 2297.2122 2297.3538 6808.6 124 4.363 26.2 1 S.GYS*GPQSSISSSLYHLSSSGSK.F YDR258C 1 1 2.7% 811 91336 8.0 U HSP78 SGDID:S000002666, Chr IV from 974237-971802, reverse complement, Verified ORF, "Oligomeric mitochondrial matrix chaperone that cooperates with Ssc1p in mitochondrial thermotolerance after heat shock; prevents the aggregation of misfolded matrix proteins; component of the mitochondrial proteolysis system" * Jamie_Mitco_SPB_55.19614.19614.2 2.623 0.2197 93.6 2396.8123 2395.5889 7518.1 28 4.244 26.2 1 Q.DEAIAAISDAVRLQRAGLT*SEK.R Reverse_YLL008W 1 1 2.7% 752 84843 5.7 U DRS1 SGDID:S000003931, Chr XII from 131728-133986, Verified ORF, "Nucleolar DEAD-box protein required for ribosome assembly and function, including synthesis of 60S ribosomal subunits; constituent of 66S pre-ribosomal particles" * Jamie_Mitco_SPB_27.09708.09708.2 1.4776 0.0676 85.4 2285.112 2286.5652 6866.7 7 3.251 34.2 1 K.ETEEEQKSDIHAMKVLGGKR.R Reverse_YOR019W 1 1 2.7% 730 83366 9.7 U YOR019W SGDID:S000005545, Chr XV from 368126-370318, Uncharacterized ORF, "Hypothetical protein" * Jamie_Mitco_SPB_85.30589.30589.2 1.7723 0.0479 88.9 2395.672 2395.6714 9284.3 303 2.678 26.3 1 K.SPVVFTPIPY*SVPFVDT*LKK.S Reverse_YOR239W 1 1 2.7% 628 71486 4.7 U ABP140 SGDID:S000005765, Chr XV from 784857-785687,785689-786744, Verified ORF, "Nonessential protein that binds actin filaments and localizes to actin patches and cables, has similarity to S-adenosylmethionine (AdoMet)-dependent methyltransferases" * Jamie_Mitco_SPB_39.13841.13841.2 1.8379 0.0564 90.4 2117.612 2114.9246 6770.7 18 2.968 31.2 1 K.NESMS*GEEEDEENT*EPK.P Reverse_YDR117C 1 1 2.7% 565 63992 8.9 U YDR117C SGDID:S000002524, Chr IV from 685638-683941, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Mitco_SPB_36.12949.12949.3 2.2256 0.0741 85.1 1726.3744 1726.7577 3981.4 269 3.318 37.5 1 K.FTQSESITTSGS*CIK.R YPL086C 1 1 2.7% 557 63657 9.0 U ELP3 SGDID:S000006007, Chr XVI from 386443-384770, reverse complement, Verified ORF, "Histone acetyltransferase subunit of the Elongator complex, which is a component of the RNA polymerase II holoenzyme; activity is directed specifically towards histones H3 and H4; disruption confers resistance to K. lactis zymotoxin" * Jamie_Mitco_SPB_93.33324.33324.2 2.0301 0.0361 91.4 2058.7522 2058.1248 7740.0 264 3.783 35.7 1 R.DIEQFKEY*FENPDFR.T YNL175C 1 1 2.7% 403 45667 9.2 U NOP13 SGDID:S000005119, Chr XIV from 308614-307403, reverse complement, Verified ORF, "Protein of unknown function, localizes to the nucleolus and nucleoplasm; contains an RNA recognition motif (RRM) and has similarity to Nop12p, which is required for processing of pre-18S rRNA" * Jamie_Mitco_SPB_12.04063.04063.1 1.1826 0.0629 94.1 1202.71 1202.3934 4786.4 410 3.26 30.0 1 R.GKVTLEELNAK.Y Reverse_YKL010C 1 1 2.6% 1483 167842 4.9 U UFD4 SGDID:S000001493, Chr XI from 425518-421067, reverse complement, Verified ORF, "Ubiquitin-protein ligase (E3) that interacts with Rpt4p and Rpt6p, two subunits of the 19S particle of the 26S proteasome; cytoplasmic E3 involved in the degradation of ubiquitin fusion proteins" * Jamie_Mitco_SPB_108.38836.38836.3 2.2346 0.2286 96.5 4509.8345 4511.045 7941.1 10 4.278 17.6 1 K.SYHQFSRTAMDVIDTKERLERS*LVDSSMALVTLIDLCK.L Reverse_YML081W 2 2 2.6% 1251 141465 7.8 U YML081W SGDID:S000004546, Chr XIII from 104777-108532, Uncharacterized ORF, "Hypothetical protein" * Jamie_Mitco_SPB_57.20344.20344.3 2.4178 0.0753 88.7 2039.9644 2041.1682 4902.1 25 3.256 35.0 1 K.ELSSNMSNWDQNWICR.L * Jamie_Mitco_SPB_19.06564.06564.2 1.9555 0.2471 87.5 1911.7922 1913.7765 6302.3 20 4.187 31.2 1 K.QSSDT*WGPGSQTTAT*QT.T Reverse_YIL147C 1 2 2.6% 1220 134435 7.5 U SLN1 SGDID:S000001409, Chr IX from 73453-69791, reverse complement, Verified ORF, "Histidine kinase osmosensor that regulates a MAP kinase cascade; transmembrane protein with an intracellular kinase domain that signals to Ypd1p and Ssk1p, thereby forming a phosphorelay system similar to bacterial two-component regulators" * Jamie_Mitco_SPB_60.21411.21411.3 3.022 0.1764 98.1 3497.6643 3498.789 9045.0 36 3.417 18.5 2 K.SRVEPLVDNTLSSRNTAPTAVSST*SQRSKISK.A YOR109W 2 2 2.6% 1107 124577 7.2 U INP53 SGDID:S000005635, Chr XV from 525278-528601, Verified ORF, "Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in a TGN (trans Golgi network)-to-early endosome pathway; hyperosmotic stress causes translocation to actin patches" * Jamie_Mitco_SPB_90.32395.32395.2 1.713 0.0463 88.0 2043.6322 2043.3458 7169.0 53 2.867 28.9 1 K.SSFSRKGKMSLAGALSDATK.S * Jamie_Mitco_SPB_10.03267.03267.2 1.6212 0.0034 84.4 1070.5322 1070.1906 4772.1 122 2.699 56.2 1 R.INLPNEDVR.R YJR091C 2 2 2.6% 1091 120374 7.0 U JSN1 SGDID:S000003851, Chr X from 598250-594975, reverse complement, Verified ORF, "Member of the Puf family of RNA-binding proteins, interacts with mRNAs encoding membrane-associated proteins; overexpression suppresses a tub2-150 mutation and causes increased sensitivity to benomyl in wild-type cells" * Jamie_Mitco_SPB_25.08694.08694.3 2.415 0.0425 81.6 1936.5543 1940.0032 5227.1 434 3.033 31.2 1 R.NNTMSSQIT*S*ISSIAPK.P * Jamie_Mitco_SPB_51.18041.18041.2 1.5409 0.0076 82.1 1265.9122 1265.4551 3635.6 146 3.501 45.0 1 K.YGEVISARTLR.N Reverse_YIL031W 2 2 2.6% 1034 116882 6.8 U ULP2 SGDID:S000001293, Chr IX from 292632-295736, Verified ORF, "Peptidase that deconjugates Smt3/SUMO-1 peptides from proteins, plays a role in chromosome cohesion at centromeric regions and recovery from checkpoint arrest induced by DNA damage or DNA replication defects; potential Cdc28p substrate" * Jamie_Mitco_SPB_117.41968.41968.1 1.1165 0.0649 92.2 745.42 744.9126 2815.1 63 2.925 58.3 1 K.KGLSAIR.T * Jamie_Mitco_SPB_122.43588.43588.3 2.364 0.0576 82.1 2377.1943 2374.6775 8864.4 2 3.075 31.6 1 R.SSNLT*DLPKLSNFKRKRASM.- YLR187W 2 2 2.6% 1026 114111 8.3 U SKG3 SGDID:S000004177, Chr XII from 524867-527947, Uncharacterized ORF, "Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; potential Cdc28p substrate; similar to Caf120p and Skg4p" * Jamie_Mitco_SPB_18.06133.06133.2 1.2869 0.06 82.7 824.9322 824.011 4939.8 13 2.761 75.0 1 K.KLSKGYK.G * Jamie_Mitco_SPB_37.13093.13093.2 1.986 0.107 97.3 2283.0122 2283.4136 9231.8 205 3.847 28.9 1 R.Y*VGTSAESKTFEIGNVRESK.S Reverse_YHR073W 2 2 2.6% 996 113760 8.6 U OSH3 SGDID:S000001115, Chr VIII from 242584-245574, Verified ORF, "Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability" * Jamie_Mitco_SPB_113.40548.40548.1 1.3552 0.0165 89.1 1052.18 1053.0734 4798.9 7 3.001 62.5 1 K.DVNGEEYAR.I * Jamie_Mitco_SPB_101.36094.36094.2 1.668 0.0968 91.5 2041.4922 2040.1599 5800.7 8 3.299 31.2 1 R.RHHEEINSSS*GKHLLSK.T YAL035W 2 2 2.6% 1002 112268 5.8 U FUN12 SGDID:S000000033, Chr I from 76428-79436, Verified ORF, "GTPase, required for general translation initiation by promoting Met-tRNAiMet binding to ribosomes and ribosomal subunit joining; homolog of bacterial IF2" * Jamie_Mitco_SPB_89.32017.32017.3 2.398 0.0754 84.9 2817.1443 2819.0151 5963.9 176 2.983 23.0 1 K.AAAEKSQKSKGES*DKPS*ASAKKPAKK.V * Jamie_Mitco_SPB_63.22569.22569.2 1.651 0.0201 83.5 1797.3322 1796.9321 5640.3 32 3.088 34.4 1 K.SKGESDKPSAS*AKKPAK.K Reverse_YCL054W 1 1 2.6% 841 96485 8.0 U SPB1 SGDID:S000000559, Chr III from 31449-33974, Verified ORF, "AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the putative tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants" * Jamie_Mitco_SPB_91.32635.32635.2 1.7716 0.11 94.7 2401.4922 2403.7961 7527.4 127 3.119 23.8 1 K.PAKFGKCVVFIEASVNRSAPPK.T YDR485C 1 1 2.6% 795 90580 5.2 U VPS72 SGDID:S000002893, Chr IV from 1427197-1424810, reverse complement, Verified ORF, "Protein of unknown function, component of the Swr1p complex that incorporates Htz1p into chromatin; required for vacuolar protein sorting" * Jamie_Mitco_SPB_122.43660.43660.3 2.0472 0.1177 86.6 2506.5244 2503.938 9379.6 415 3.772 26.2 1 R.RTSKRSSVMENTMKVYEKLSK.A YGR013W 1 1 2.6% 620 71383 5.0 U SNU71 SGDID:S000003245, Chr VII from 514558-516420, Verified ORF, "Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart" * Jamie_Mitco_SPB_41.14621.14621.2 1.5416 0.0038 80.8 1985.7722 1988.2047 8765.2 4 2.971 33.3 1 K.IELHLDLNTKEYVEDR.T Reverse_YJR045C 1 1 2.6% 654 70628 5.6 U SSC1 SGDID:S000003806, Chr X from 521516-519552, reverse complement, Verified ORF, "Mitochondrial matrix ATPase that is a subunit of the presequence translocase-associated protein import motor (PAM); involved in protein translocation into the matrix and protein folding; member of the heat shock protein 70 (HSP70) family" * Jamie_Mitco_SPB_103.37045.37045.2 1.5818 0.0109 81.4 1818.8722 1818.9353 5794.0 178 3.564 31.2 1 R.IEVST*QGAAATSFIQSK.K YDL203C 1 1 2.6% 623 69371 8.4 U YDL203C SGDID:S000002362, Chr IV from 97954-96083, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Mitco_SPB_17.06004.06004.2 2.0994 0.1489 98.9 1747.8322 1748.8939 7349.3 31 3.634 43.3 1 R.GTSSLDPSPTKEQCIK.K Reverse_YKL203C 3 3 2.5% 2474 281566 7.5 U TOR2 SGDID:S000001686, Chr XI from 63360-55936, reverse complement, Verified ORF, "PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis" * Jamie_Mitco_SPB_109.39149.39149.2 1.6046 0.17 97.0 2437.8523 2437.5977 6298.0 7 3.903 30.6 1 K.LINQNVPLKT*VQMEDEY*NK.R * Jamie_Mitco_SPB_22.07811.07811.2 1.4093 0.1341 90.3 2607.2522 2608.7424 9665.2 20 3.554 27.1 1 K.GGVVSLEGLVKLAT*S*AVASSADQCK.P * Jamie_Mitco_SPB_39.13737.13737.2 2.1405 0.213 86.4 1947.5521 1949.0813 5606.9 193 4.188 31.2 1 V.EFEVFDSTLT*GGPVTLR.G YPL082C 2 2 2.5% 1867 209975 6.6 U MOT1 SGDID:S000006003, Chr XVI from 404080-398477, reverse complement, Verified ORF, "Essential abundant protein involved in regulation of transcription, removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity" * Jamie_Mitco_SPB_69.24750.24750.3 2.7028 0.1042 92.9 3532.1943 3532.0461 6998.8 57 2.699 19.8 1 R.MLAMARRKKKMS*AKNTPKHPVDITES*SVSK.T * Jamie_Mitco_SPB_62.22306.22306.2 2.1023 0.2373 89.4 1983.4922 1981.126 6801.6 12 4.257 37.5 1 K.HPEGEKLPSFVS*EIFSP.V Reverse_YBL005W-B 2 2 2.5% 1755 198968 8.2 U YBL005W-B SGDID:S000002147, Chr II from 221333-222637,222639-226601, transposable_element_gene, "TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition" Reverse_YMR045C 2 2 2.5% 1755 198898 8.3 U YMR045C SGDID:S000004648, Chr XIII from 362626-361322,361320-357358, reverse complement, transposable_element_gene, "TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition" Jamie_Mitco_SPB_36.12777.12777.2 2.1025 0.0647 96.2 1539.0322 1539.7716 5702.6 1 2.815 58.3 1 R.FKYGVYS*ALGILK.Q Jamie_Mitco_SPB_104.37175.37175.3 2.1711 0.1282 89.8 3887.9343 3885.2654 9779.8 142 3.6 16.7 1 K.VWPPLFQS*PAFLQFTHCLFTLEDDT*MQRVAK.R YGR109W-B 1 1 2.5% 1547 178419 8.8 U YGR109W-B SGDID:S000007347, Chr VII from 707614-708463,708465-712258, transposable_element_gene, "TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition" YIL082W-A 1 1 2.5% 1498 172454 9.2 U YIL082W-A SGDID:S000003537, Chr IX from 205632-206482,206484-210129, transposable_element_gene, "Hypothetical protein" Jamie_Mitco_SPB_39.13972.13972.4 3.5278 0.1655 96.0 4472.0166 4475.0093 8069.1 340 4.534 15.3 1 R.DIVELLKY*EIYETPPLRFRGFVATKSAVTSEAVT*IDLK.I Reverse_YFL004W 1 1 2.5% 828 95441 6.8 U VTC2 SGDID:S000001890, Chr VI from 131805-134291, Verified ORF, "Vacuolar membrane protein involved in vacuolar polyphosphate accumulation; functions as a regulator of vacuolar H+-ATPase activity and vacuolar transporter chaperones; involved in protein localization and non-autophagic vacuolar fusion" * Jamie_Mitco_SPB_91.32550.32550.2 1.7047 0.1105 93.1 2458.652 2460.6978 6696.3 2 3.458 32.5 1 K.LQLKTLDFESKGTAEDEILT*K.K YOR119C 1 1 2.5% 484 56122 6.6 U RIO1 SGDID:S000005645, Chr XV from 550246-548792, reverse complement, Verified ORF, "Essential serine kinase involved in cell cycle progression and processing of the 20S pre-rRNA into mature 18S rRNA" * Jamie_Mitco_SPB_121.43463.43463.3 2.2034 0.1369 93.0 1326.9543 1329.5852 8347.0 83 3.609 43.2 1 K.RIYQSGVIPAPK.P YPR041W 1 1 2.5% 405 45261 5.1 U TIF5 SGDID:S000006245, Chr XVI from 648701-649918, Verified ORF, "Translation initiation factor eIF-5; N-terminal domain functions as a GTPase-activating protein to mediate hydrolysis of ribosome-bound GTP; C-terminal domain is the core of ribosomal preinitiation complex formation" * Jamie_Mitco_SPB_28.09801.09801.2 1.3103 0.0693 82.6 990.39215 988.0422 3755.7 60 3.067 55.6 1 K.NPPDSVSGSK.K YBR136W 3 3 2.4% 2368 273340 8.3 U MEC1 SGDID:S000000340, Chr II from 505662-512768, Verified ORF, "Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; monitors and participates in meiotic recombination" * Jamie_Mitco_SPB_58.20561.20561.2 2.1577 0.0804 97.2 2215.0122 2216.641 6827.8 4 3.18 36.1 1 K.T*VSALALLLEYNPFLLVMK.D * Jamie_Mitco_SPB_17.06101.06101.2 1.2908 0.1003 84.4 2304.132 2305.4656 6829.0 267 3.418 26.5 1 R.LIDDFLNISVLNYDNY*HR.I * Jamie_Mitco_SPB_104.37114.37114.2 1.6288 0.0396 85.5 2329.5723 2330.6277 4560.5 54 3.467 26.3 1 K.PKQLNIIGS*DGNIYGIMCKK.E YMR207C 2 2 2.4% 2123 241784 8.0 U HFA1 SGDID:S000004820, Chr XIII from 683563-677192, reverse complement, Verified ORF, "Mitochondrial acetyl-coenzyme A carboxylase, catalyzes the production of malonyl-CoA in mitochondrial fatty acid biosynthesis" * Jamie_Mitco_SPB_64.22743.22743.3 2.4767 0.1223 92.6 3829.3442 3825.245 7291.6 118 3.557 17.5 1 K.MSNYKIRSLPTYDS*SIRIFEGISKFT*PLDKR.F * Jamie_Mitco_SPB_20.07179.07179.4 2.0919 0.1541 80.9 2357.9766 2363.6829 5248.6 438 3.928 26.9 1 R.RGNIVLEELT*RLVDSKRKR.- Reverse_YBR275C 2 2 2.4% 1916 217959 6.6 U RIF1 SGDID:S000000479, Chr II from 757101-751351, reverse complement, Verified ORF, "Protein that binds to the Rap1p C-terminus and acts synergistically with Rif2p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation" * Jamie_Mitco_SPB_8.02820.02820.2 1.9416 0.0269 92.8 1035.6122 1034.1112 3958.8 33 2.84 62.5 1 R.KNESTPTEK.S * Jamie_Mitco_SPB_75.26771.26771.4 3.5352 0.1984 97.7 4168.6567 4171.54 6222.3 1 3.799 19.4 1 K.ASIQSSIQISSPPEKMGQT*APVQIDQS*DVKRQLNSLK.N Reverse_YPL082C 1 1 2.4% 1867 209975 6.6 U MOT1 SGDID:S000006003, Chr XVI from 404080-398477, reverse complement, Verified ORF, "Essential abundant protein involved in regulation of transcription, removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity" * Jamie_Mitco_SPB_48.16989.16989.4 3.4672 0.1342 90.6 4866.0166 4863.1416 5475.4 327 3.365 13.6 1 K.VQANDLPS*GENTGGVLDSENPDWSPAHAVIGGVARAAT*VRTEWKK.V YLR096W 2 2 2.4% 1147 128338 9.4 U KIN2 SGDID:S000004086, Chr XII from 332591-336034, Verified ORF, "Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin1p" * Jamie_Mitco_SPB_15.05327.05327.2 1.7812 0.033 89.6 1123.3322 1123.2511 6407.5 43 2.943 55.6 1 R.TISDYIPSAR.R Jamie_Mitco_SPB_24.08389.08389.2 1.753 0.0342 87.9 1866.6921 1865.1826 5266.2 51 2.889 37.5 1 K.GFFSVQTTSSKPLPIVR.H Reverse_YBR033W 2 2 2.4% 919 103397 8.6 U YBR033W SGDID:S000000237, Chr II from 301944-304703, Uncharacterized ORF, "Non-essential protein of unknown function" * Jamie_Mitco_SPB_104.37150.37150.2 1.7642 0.0366 87.7 1844.0521 1841.8181 8880.4 37 3.495 30.0 1 R.SSVSQNVEGMS*AS*NRK.R * Jamie_Mitco_SPB_14.04840.04840.2 1.562 0.1649 98.4 808.33215 809.86005 3387.5 2 3.452 80.0 1 K.REHPDR.I YBR237W 1 1 2.4% 849 96359 8.1 U PRP5 SGDID:S000000441, Chr II from 691964-694513, Verified ORF, "RNA helicase in the DEAD-box family, necessary for prespliceosome formation, bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA" * Jamie_Mitco_SPB_39.13919.13919.2 1.4025 0.073 84.4 2547.912 2548.7756 8974.0 3 3.129 31.6 1 K.RKNEFRKSDEPVS*VKPS*KKK.S Reverse_YER136W 1 1 2.4% 451 51206 6.0 U GDI1 SGDID:S000000938, Chr V from 439612-440967, Verified ORF, "GDP dissociation inhibitor, regulates vesicle traffic in secretory pathways by regulating the dissociation of GDP from the Sec4/Ypt/rab family of GTP binding proteins" * Jamie_Mitco_SPB_16.05752.05752.2 1.6112 0.0037 82.5 1290.8322 1290.3727 7009.2 253 2.999 50.0 1 K.TKVGEFKGT*DK.K YDR229W 1 1 2.4% 453 49976 5.0 U IVY1 SGDID:S000002637, Chr IV from 924779-926140, Verified ORF, "Phospholipid-binding protein that interacts with both Ypt7p and Vps33p, may partially counteract the action of Vps33p and vice versa, localizes to the rim of the vacuole as cells approach stationary phase" * Jamie_Mitco_SPB_11.03964.03964.2 2.6239 0.2476 100.0 1256.0721 1256.3116 5325.1 3 5.073 65.0 1 R.NSAEETTQTFK.Q Reverse_YDR127W 2 2 2.3% 1588 174754 6.3 U ARO1 SGDID:S000002534, Chr IV from 704479-709245, Verified ORF, "Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids" * Jamie_Mitco_SPB_74.26590.26590.2 1.81 0.1077 95.7 1920.9722 1918.2058 5954.8 236 3.837 31.2 1 K.HPLGLIEYGTNHLIPSR.S * Jamie_Mitco_SPB_19.06827.06827.3 2.834 0.1318 94.5 2143.0745 2143.501 6389.9 245 3.398 27.6 1 K.IKCQGEGLAALILARNSISK.S Reverse_YOR116C 1 1 2.3% 1460 162301 8.2 U RPO31 SGDID:S000005642, Chr XV from 544145-539763, reverse complement, Verified ORF, "RNA polymerase III subunit C160, part of core enzyme; similar to bacterial beta-prime subunit" * Jamie_Mitco_SPB_83.29884.29884.4 3.2185 0.0892 81.9 3794.8965 3796.1484 6552.5 422 2.688 18.2 1 R.DPVAVEDISLNPDPSIVTRGSFDVRKGS*LNGRFR.G Reverse_YLR086W 2 2 2.3% 1418 162188 6.2 U SMC4 SGDID:S000004076, Chr XII from 302244-306500, Verified ORF, "Subunit of the condensin complex, which reorganizes chromosomes during cell division, forms a stable complex with Smc2p that has ATP-hydrolyzing and DNA-binding activity and promotes knotting of circular DNA; potential Cdc28p substrate" * Jamie_Mitco_SPB_58.20628.20628.2 1.5753 0.0359 83.8 2446.9521 2449.6062 9286.8 1 3.153 28.9 1 R.LEELMNEAESISHKT*S*KLTK.E * Jamie_Mitco_SPB_99.35471.35471.2 1.9 0.2325 83.0 1645.6322 1643.6581 5452.9 6 4.129 45.8 1 K.TVETYS*SEKENIY.Y YDL145C 1 1 2.3% 1201 135607 6.0 U COP1 SGDID:S000002304, Chr IV from 198177-194572, reverse complement, Verified ORF, "Alpha subunit of COPI vesicle coatomer complex, which surrounds transport vesicles in the early secretory pathway" * Jamie_Mitco_SPB_42.15123.15123.3 2.7788 0.0323 82.7 3273.2644 3276.736 7596.8 196 3.423 22.2 1 K.Y*VITEKDKIDRIAMISKIGAPAS*GLRIR.V YPL226W 2 2 2.3% 1196 134331 5.9 U NEW1 SGDID:S000006147, Chr XVI from 121767-125357, Verified ORF, "ATP binding cassette family member; Asn/Gln-rich rich region supports [NU+] prion formation, susceptibility to [PSI+] prion induction and aggregation of a fragment of the human Machado-Joseph Disease protein" * Jamie_Mitco_SPB_120.43003.43003.2 1.4978 0.0598 85.5 1578.5922 1575.6262 7972.1 108 2.985 41.7 1 R.EDSANDLLELT*FK.D * Jamie_Mitco_SPB_10.03579.03579.3 2.4687 0.0647 89.6 1801.9443 1799.0896 6722.7 161 3.188 30.8 1 R.LTRNEKKLQAERRR.L Reverse_YPR181C 1 1 2.3% 768 85385 5.7 U SEC23 SGDID:S000006385, Chr XVI from 899663-897357, reverse complement, Verified ORF, "GTPase-activating protein; component of the Sec23p-Sec24p heterodimeric complex of the COPII vesicle coat, involved in ER to Golgi transport and autophagy; stimulates the GDP-bound form of Sar1p" * Jamie_Mitco_SPB_96.34264.34264.2 1.8268 0.0349 88.6 1935.7322 1933.1722 5396.1 174 3.351 32.4 1 K.VASAHGILGQVKLDKST*K.V YMR224C 1 1 2.3% 692 77650 5.9 U MRE11 SGDID:S000004837, Chr XIII from 720652-718574, reverse complement, Verified ORF, "Subunit of a complex with Rad50p and Xrs2p (RMX complex) that functions in repair of DNA double-strand breaks and in telomere stability, exhibits nuclease activity that appears to be required for RMX function; widely conserved" * Jamie_Mitco_SPB_13.04381.04381.2 1.8563 0.1799 98.9 1823.7122 1823.7844 5204.5 2 4.004 46.7 1 R.T*GSPDITQSHVDNESR.I Reverse_YDR194C 1 1 2.3% 664 76269 9.0 U MSS116 SGDID:S000002602, Chr IV from 847941-845947, reverse complement, Verified ORF, "DEAD-box protein required for efficient splicing of mitochondrial Group I and II introns; presumed RNA helicase due to DEAD-box motif" * Jamie_Mitco_SPB_98.35158.35158.2 1.6473 0.0010 82.1 1943.8722 1943.1731 5251.1 194 2.994 35.7 1 K.KFENKLISCLFST*FK.V Reverse_YPR171W 1 1 2.3% 576 64666 9.9 U BSP1 SGDID:S000006375, Chr XVI from 883824-885554, Verified ORF, "Adapter that links synaptojanins Inp2p and Inp53p to the cortical actin cytoskeleton" * Jamie_Mitco_SPB_68.24293.24293.2 1.8633 0.0348 89.6 1641.4722 1638.6854 4753.0 34 3.511 41.7 1 K.TEKVTEDRSTTY*K.D YMR024W 1 1 2.3% 390 44000 9.4 U MRPL3 SGDID:S000004626, Chr XIII from 321874-323046, Verified ORF, "Mitochondrial ribosomal protein of the large subunit" * Jamie_Mitco_SPB_108.38673.38673.1 1.5148 0.0465 95.7 945.4 946.0886 3921.2 120 3.095 56.2 1 K.DGIELITGK.N YFR019W 3 3 2.2% 2278 257447 8.3 U FAB1 SGDID:S000001915, Chr VI from 184490-191326, Verified ORF, "1-phosphatidylinositol-3-phosphate 5-kinase; vacuolar membrane kinase that generates phosphatidylinositol (3,5)P2, which is involved in vacuolar sorting and homeostasis" * Jamie_Mitco_SPB_92.33022.33022.2 2.0968 0.2395 90.8 2035.8522 2035.1266 6379.5 25 4.152 31.6 1 V.GDEVSSIKS*ASSSLTASLSK.S * Jamie_Mitco_SPB_10.03516.03516.1 1.3606 0.0422 94.9 929.47 929.9163 4817.2 16 2.976 66.7 1 R.KDS*EDKK.S * Jamie_Mitco_SPB_89.31979.31979.2 1.7348 0.1316 96.3 2668.872 2669.8218 9678.7 127 3.935 22.7 1 R.DRTPQESQTHSQANLDTLQELEK.I Reverse_YNL271C 2 2 2.2% 1953 219701 6.5 U BNI1 SGDID:S000005215, Chr XIV from 135384-129523, reverse complement, Verified ORF, "Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1" * Jamie_Mitco_SPB_86.30713.30713.2 2.2181 0.2305 90.3 2304.0122 2306.4949 6044.4 81 4.511 27.5 1 K.RAS*SPDSKAPGANKLQELLKD.M * Jamie_Mitco_SPB_27.09397.09397.2 1.3243 0.1119 86.9 2410.2922 2409.3555 9900.9 26 3.572 28.6 1 K.QSLADLFSGPGS*GSSLESHES*R.L YLR256W 2 2 2.2% 1502 166107 7.4 U HAP1 SGDID:S000004246, Chr XII from 646417-650925, Verified ORF, "Zinc finger transcription factor involved in the complex regulation of gene expression in response to levels of heme and oxygen; the S288C sequence differs from other strain backgrounds due to a Ty1 insertion in the carboxy terminus" * Jamie_Mitco_SPB_101.36345.36345.2 2.3087 0.282 88.9 2242.392 2245.293 7367.5 14 4.775 32.4 1 F.Y*NFY*MKSASNDLTTLTNT.N * Jamie_Mitco_SPB_96.34465.34465.2 1.7418 0.1233 96.2 1670.8922 1668.8218 5087.0 148 3.447 39.3 1 K.HPKVS*NMSGFLAGDK.D Reverse_YHR158C 1 1 2.2% 1164 131094 5.4 U KEL1 SGDID:S000001201, Chr VIII from 417182-413688, reverse complement, Verified ORF, "Protein required for proper cell fusion and cell morphology; functions in a complex with Kel2p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate" * Jamie_Mitco_SPB_104.37293.37293.4 2.9152 0.1463 86.3 3239.4565 3242.3955 4549.1 137 3.627 22.7 1 R.RFS*S*LDYVALDNFYTDDFQGGFVYLK.T YMR196W 1 1 2.2% 1088 126624 5.4 U YMR196W SGDID:S000004809, Chr XIII from 655075-658341, Uncharacterized ORF, "Hypothetical protein" * Jamie_Mitco_SPB_62.22175.22175.2 1.6984 0.0434 88.0 2744.8123 2746.833 8012.7 3 3.081 30.4 1 K.RHHRIGGTHS*GLTPQSS*ISSDKAR.H YER111C 1 1 2.2% 1093 123806 8.8 U SWI4 SGDID:S000000913, Chr V from 385872-382591, reverse complement, Verified ORF, "DNA binding component of the SBF complex (Swi4p-Swi6p), a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair" * Jamie_Mitco_SPB_49.17579.17579.2 1.3554 0.0401 80.6 2823.2722 2820.8694 7079.8 57 3.085 21.7 1 K.LAT*IVQDEESKVDMNT*NSSSHPEK.Q Reverse_YJL050W 1 1 2.2% 1073 122055 6.3 U MTR4 SGDID:S000003586, Chr X from 342438-345659, Verified ORF, "Dead-box family ATP dependent helicase required for mRNA export from the nucleus; co-factor of the exosome complex, required for 3' end formation of 5.8S rRNA" * Jamie_Mitco_SPB_95.33858.33858.4 2.9461 0.1645 92.3 2994.6565 2991.0771 5195.2 30 3.521 26.8 1 R.DICS*IAT*DQFPDLTFPYTRAENVR.K YCR092C 1 1 2.2% 1047 119753 7.4 U MSH3 SGDID:S000000688, Chr III from 279903-276760, reverse complement, Verified ORF, "Mismatch repair protein, forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability" * Jamie_Mitco_SPB_50.17904.17904.2 1.6339 0.0344 85.1 2776.632 2777.1003 8751.6 7 3.037 27.3 1 R.DEVDYLIEVKNSQIKDLPDDWIK.V YHR073W 2 2 2.2% 996 113760 8.6 U OSH3 SGDID:S000001115, Chr VIII from 242584-245574, Verified ORF, "Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability" * Jamie_Mitco_SPB_24.08327.08327.2 1.4461 0.0368 82.7 1179.2722 1179.405 6745.4 175 3.188 55.0 1 K.LSASGFTLVKR.V * Jamie_Mitco_SPB_19.06711.06711.2 1.9357 0.1475 98.9 1474.7922 1473.814 5066.5 35 3.965 50.0 1 K.RRKRLQGFKKR.F Reverse_YBR203W 2 2 2.2% 924 104504 9.6 U COS111 SGDID:S000000407, Chr II from 629163-631937, Verified ORF, "Protein required for wild-type resistance to the antifungal drug ciclopirox olamine; not related to the COS family of subtelomerically-encoded proteins" * Jamie_Mitco_SPB_38.13654.13654.2 1.6962 0.0010 83.2 1558.1322 1557.7042 7892.2 7 2.543 45.8 1 R.QKS*RSKQSISSLK.G * Jamie_Mitco_SPB_121.43523.43523.2 1.6496 0.1332 94.9 1989.5322 1989.1503 7402.6 64 3.18 35.3 1 K.SRSKQSISSLKGVTT*SNK.G YPL008W 1 1 2.2% 861 98806 7.9 U CHL1 SGDID:S000005929, Chr XVI from 539382-541967, Verified ORF, "Conserved nuclear protein required to establish sister-chromatid pairing during S-phase, probable DNA helicase with similarity to human BACH1, which associates with tumor suppressor BRCA1; associates with acetyltransferase Ctf7p" * Jamie_Mitco_SPB_61.21618.21618.2 1.6058 0.0582 87.3 2507.8323 2505.5676 5977.3 182 3.116 25.0 1 K.ENLQT*Y*ITNQPELEFTFEK.R Reverse_YFR013W 1 1 2.2% 787 90897 7.8 U IOC3 SGDID:S000001909, Chr VI from 169914-172277, Verified ORF, "Member of a complex (Isw1a) with Isw1p that has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; has homology to Esc8p, which is involved in silencing" * Jamie_Mitco_SPB_54.19368.19368.3 2.5877 0.1579 95.4 2069.8743 2071.2202 4425.4 1 3.834 37.5 1 K.IAMFDPY*LLLST*DEPGK.V YDL060W 1 1 2.2% 788 90748 5.7 U TSR1 SGDID:S000002218, Chr IV from 341619-343985, Verified ORF, "Protein required for processing of 20S pre-rRNA in the cytoplasm, associates with pre-40S ribosomal particles" * Jamie_Mitco_SPB_28.10027.10027.2 2.5334 0.2398 100.0 1990.7322 1991.1156 8031.0 54 4.665 34.4 1 R.EFPDEIELEPSESAIER.L YGL234W 1 1 2.2% 802 86068 5.3 U ADE5,7 SGDID:S000003203, Chr VII from 56482-58890, Verified ORF, "Bifunctional enzyme of the 'de novo' purine nucleotide biosynthetic pathway, contains aminoimidazole ribotide synthetase and glycinamide ribotide synthetase activities" * Jamie_Mitco_SPB_42.14812.14812.2 1.4861 0.0541 84.0 1768.8922 1769.9922 6463.4 195 3.056 26.5 1 R.SAVTVVVAAGGYPESYAK.G YKL198C 1 1 2.2% 649 72062 8.9 U PTK1 SGDID:S000001681, Chr XI from 70223-68274, reverse complement, Verified ORF, "Putative serine/threonine protein kinase that regulates spermine uptake; involved in polyamine transport; possible mitochondrial protein" * Jamie_Mitco_SPB_15.05255.05255.2 1.7129 0.0111 84.4 1648.7722 1648.9033 5753.8 24 2.745 42.3 1 K.KLLSSS*HGNKLKKK.A Reverse_YDL224C 1 1 2.2% 649 70690 7.0 U WHI4 SGDID:S000002383, Chr IV from 56347-54398, reverse complement, Verified ORF, "Putative RNA binding protein and partially redundant Whi3p homolog that regulates the cell size requirement for passage through Start and commitment to cell division" * Jamie_Mitco_SPB_15.05057.05057.2 2.6121 0.2789 96.7 1832.5122 1831.9425 6747.7 6 4.572 46.2 1 R.RFGQQNS*FLQRLEQ.E YPL228W 1 1 2.2% 549 61850 5.6 U CET1 SGDID:S000006149, Chr XVI from 118382-120031, Verified ORF, "Beta (RNA 5'-triphosphatase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CEG1, a guanylyltransferase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide" * Jamie_Mitco_SPB_39.13897.13897.2 2.2367 0.2421 92.0 1414.4122 1416.4875 7409.8 1 4.249 63.6 1 K.SRQSETTHEVEL.E YDR182W 1 1 2.2% 491 57147 9.4 U CDC1 SGDID:S000002590, Chr IV from 827577-829052, Verified ORF, "Putative membrane protein of unknown function involved in Mn2+ homeostasis; mutants display actin and general growth defects, heterogeneous cell cycle arrests, and pleiotropic defects in cell cycle progression and organelle distribution" * Jamie_Mitco_SPB_29.10149.10149.2 2.7949 0.1031 88.5 1233.1721 1232.5089 6084.9 4 3.538 70.0 1 R.SIKQFLVNAIV.L YGL119W 1 1 2.2% 501 56742 9.3 U ABC1 SGDID:S000003087, Chr VII from 284448-285953, Verified ORF, "Protein required for ubiquinone (coenzyme Q) biosynthesis and for respiratory growth; exhibits genetic interaction with COQ9, suggesting a common function; similar to prokaryotic proteins involved in early steps of ubiquinone biosynthesis" * Jamie_Mitco_SPB_15.05365.05365.1 1.2617 0.0228 88.7 1430.2 1430.6041 4842.4 79 2.981 40.0 1 K.LLTYAT*LRDRK.G Reverse_YDR229W 1 1 2.2% 453 49976 5.0 U IVY1 SGDID:S000002637, Chr IV from 924779-926140, Verified ORF, "Phospholipid-binding protein that interacts with both Ypt7p and Vps33p, may partially counteract the action of Vps33p and vice versa, localizes to the rim of the vacuole as cells approach stationary phase" * Jamie_Mitco_SPB_47.16858.16858.2 1.5681 0.0011 82.6 1340.6921 1342.3683 5561.2 83 2.684 50.0 1 R.RAKS*YVKSS*R.L YDR150W 3 3 2.1% 2748 313032 5.4 U NUM1 SGDID:S000002557, Chr IV from 755623-763869, Verified ORF, "Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex" * Jamie_Mitco_SPB_81.28984.28984.2 1.7708 0.1373 96.9 2478.3123 2476.7466 9969.7 18 4.047 28.6 1 R.LLISVSDLTSEINSLQSNRTER.I * Jamie_Mitco_SPB_97.34747.34747.3 2.2381 0.0677 81.1 2343.2344 2343.5684 4277.6 337 2.945 26.3 1 K.NLLQKSESASKPKDDELMT*K.E * Jamie_Mitco_SPB_66.23509.23509.2 2.4715 0.2077 91.5 1993.2322 1996.0574 6441.1 1 4.493 43.8 1 K.EDKKGQATASKHEY*VSH.K YLR342W 2 2 2.1% 1876 214849 7.2 U FKS1 SGDID:S000004334, Chr XII from 809997-815627, Verified ORF, "Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic subunit Gsc2p; binds to regulatory subunit Rho1p; involved in cell wall synthesis and maintenance; localizes to sites of cell wall remodeling" * Jamie_Mitco_SPB_14.04987.04987.2 3.7216 0.3074 100.0 1823.6122 1821.9075 6641.7 1 5.204 60.0 1 K.AMEEANPEDTEETLNK.I * Jamie_Mitco_SPB_16.05733.05733.2 2.7401 0.145 100.0 2538.9722 2538.6814 9586.4 1 5.551 36.4 1 K.ILAEETAAYEGNENEAEKEDALK.S Reverse_YHR164C 2 2 2.1% 1522 171694 6.5 U DNA2 SGDID:S000001207, Chr VIII from 429180-424612, reverse complement, Verified ORF, "Essential tripartite DNA replication factor with single-stranded DNA-dependent ATPase, ATP-dependent nuclease, and helicase activities; required for Okazaki fragment processing; involved in DNA repair pathways; potential Cdc28p substrate" * Jamie_Mitco_SPB_120.43149.43149.2 1.5721 0.0111 81.0 1880.8121 1882.9823 8276.4 2 3.332 36.7 1 K.DSVFKS*KST*IPKAGTR.K * Jamie_Mitco_SPB_40.14268.14268.2 1.9955 0.0511 92.3 2205.372 2203.4587 11035.8 13 3.372 36.7 1 K.DRTFY*LLFFEIPIEYR.D Reverse_YDR159W 2 2 2.1% 1301 149568 8.9 U SAC3 SGDID:S000002566, Chr IV from 771872-775777, Verified ORF, "Nuclear pore-associated protein, forms a complex with Thp1p that is involved in transcription and in mRNA export from the nucleus" * Jamie_Mitco_SPB_120.43117.43117.2 1.602 0.0359 84.9 1632.6721 1633.5977 10408.6 177 3.224 38.5 1 K.DSSS*LMFS*SNVNGK.Y * Jamie_Mitco_SPB_72.25680.25680.2 1.7044 0.0463 88.5 1409.9722 1412.4216 4419.1 11 3.156 45.8 1 R.FEAENPCTGGSSR.V YJL074C 1 1 2.1% 1230 141336 5.8 U SMC3 SGDID:S000003610, Chr X from 302765-299073, reverse complement, Verified ORF, "Subunit of the multiprotein cohesin complex required for sister chromatid cohesion in mitotic cells; also required, with Rec8p, for cohesion and recombination during meiosis; phylogenetically conserved SMC chromosomal ATPase family member" * Jamie_Mitco_SPB_26.09059.09059.3 3.75 0.2326 95.2 2962.1943 2962.0732 7480.3 167 4.241 22.0 1 T.GVSISVS*FNS*KQNEQLHVEQLSGGQK.T YBR023C 1 1 2.1% 1165 131601 6.7 U CHS3 SGDID:S000000227, Chr II from 287925-284428, reverse complement, Verified ORF, "Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan" * Jamie_Mitco_SPB_33.11662.11662.2 1.508 0.0558 84.9 3092.3523 3094.327 7091.5 13 2.72 27.1 1 K.IANKAQSWVTAIQVFEY*Y*ISHHQAK.A YPL195W 1 1 2.1% 932 106924 5.1 U APL5 SGDID:S000006116, Chr XVI from 176222-179020, Verified ORF, "Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function" * Jamie_Mitco_SPB_103.36873.36873.2 1.7714 0.0263 87.2 2449.9722 2452.8652 9431.3 135 2.855 26.3 1 R.YISCILFYKIGKINTDFISR.F YOL006C 1 1 2.1% 769 89995 8.7 U TOP1 SGDID:S000005366, Chr XV from 315387-313078, reverse complement, Verified ORF, "Topoisomerase I, nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination" * Jamie_Mitco_SPB_22.07582.07582.2 2.5502 0.0396 97.4 1967.1122 1967.3219 7122.2 1 4.228 43.3 1 K.KFEKARQLKSYIDAIR.R YKL104C 1 1 2.1% 717 80047 6.4 U GFA1 SGDID:S000001587, Chr XI from 245017-242864, reverse complement, Verified ORF, "Glutamine-fructose-6-phosphate amidotransferase, catalyzes the formation of glucosamine-6-P and glutamate from fructose-6-P and glutamine in the first step of chitin biosynthesis" * Jamie_Mitco_SPB_30.10594.10594.2 2.5462 0.1953 100.0 1672.9521 1672.879 7572.3 4 4.524 53.6 1 R.SRGEIIDTLVDGLQR.L YLR002C 1 1 2.1% 663 75582 5.9 U NOC3 SGDID:S000003992, Chr XII from 156333-154342, reverse complement, Verified ORF, "Protein that forms a nuclear complex with Noc2p that binds to 66S ribosomal precursors to mediate their intranuclear transport; also binds to chromatin to promote the association of DNA replication factors and replication initiation" * Jamie_Mitco_SPB_98.35040.35040.2 1.718 0.0652 89.9 1671.3322 1673.011 5234.1 1 4.094 53.8 1 K.AELLLKALDHVFFR.S Reverse_YIR003W 1 1 2.1% 679 74763 5.0 U YIR003W SGDID:S000001442, Chr IX from 360882-362921, Uncharacterized ORF, "Hypothetical protein" * Jamie_Mitco_SPB_53.18779.18779.2 1.6419 0.0062 82.6 1555.3522 1554.8016 5392.8 17 2.516 42.3 1 R.SPREPVKPTVESPM.- YMR064W 1 1 2.1% 518 59748 9.3 U AEP1 SGDID:S000004668, Chr XIII from 397902-399458, Verified ORF, "Protein required for expression of the mitochondrial OLI1 gene encoding subunit 9 of F1-F0 ATP synthase" * Jamie_Mitco_SPB_121.43269.43269.2 1.4952 0.1138 90.9 1198.0122 1197.4203 6680.8 16 3.497 50.0 1 K.GILPEATIINR.Y Reverse_YML059C 2 2 2.0% 1679 187132 8.1 U NTE1 SGDID:S000004524, Chr XIII from 158258-153219, reverse complement, Verified ORF, "Serine esterase that deacylates exogenous lysophospholipids, homolog of human neuropathy target esterase (NTE); mammalian NTE1 deacylates phosphatidylcholine to glycerophosphocholine" * Jamie_Mitco_SPB_115.41045.41045.2 1.5599 0.0352 83.3 1904.0322 1902.0287 3437.1 99 2.977 32.1 1 K.ISDPLLKS*INQQT*KR.S * Jamie_Mitco_SPB_121.43362.43362.2 1.9873 0.1886 99.6 2189.7122 2191.4614 7255.7 159 4.485 27.8 1 K.MAFAEVPLGST*IPLIT*ITR.F Reverse_YBR059C 1 1 2.0% 1108 123989 7.7 U AKL1 SGDID:S000000263, Chr II from 360185-356859, reverse complement, Verified ORF, "Ser-Thr protein kinase, member (with Ark1p and Prk1p) of the Ark kinase family; involved in endocytosis and actin cytoskeleton organization" * Jamie_Mitco_SPB_16.05431.05431.3 2.9324 0.2315 99.4 2340.7144 2340.4375 5441.7 27 4.076 27.4 1 K.ENALAEDSGITAAGY*STSKMSR.G Reverse_YLL003W 1 1 2.0% 946 112978 9.8 U SFI1 SGDID:S000003926, Chr XII from 143200-146040, Verified ORF, "Centrin (Cdc31p)-binding protein required for spindle pole body (SPB) duplication, localizes to the half-bridge of the SPB, required for progression through G(2)-M transition, has similarity to Xenopus laevis XCAP-C" * Jamie_Mitco_SPB_38.13501.13501.4 2.7226 0.139 86.2 2403.0166 2404.7288 4559.6 182 3.177 30.6 1 R.VKEAIS*KNYEYNIVKQKIR.E Reverse_YDR027C 1 1 2.0% 889 101520 6.3 U VPS54 SGDID:S000002434, Chr IV from 497314-494645, reverse complement, Verified ORF, "Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; potentially phosphorylated by Cdc28p" * Jamie_Mitco_SPB_112.40158.40158.2 1.5271 0.0641 86.2 2213.7122 2213.3262 5535.6 248 3.263 23.5 1 K.DNS*GTQIRHLTEKPKIS*K.C YMR053C 1 1 2.0% 850 97828 6.7 U STB2 SGDID:S000004657, Chr XIII from 382897-380345, reverse complement, Verified ORF, "Protein that interacts with Sin3p in a two-hybrid assay and is part of a large protein complex with Sin3p and Stb1p" * Jamie_Mitco_SPB_39.13761.13761.2 1.6622 0.0069 82.6 2302.652 2305.3516 10824.5 190 2.72 28.1 1 K.CCTDISNEEFLKFNY*QR.G Reverse_YPL158C 1 1 2.0% 758 84846 6.7 U YPL158C SGDID:S000006079, Chr XVI from 254309-252033, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Mitco_SPB_41.14655.14655.2 2.1913 0.204 84.6 1798.1721 1798.6909 11611.7 23 4.989 39.3 1 K.SSVSSSYRSNSFRS*S*.E YKR067W 1 1 2.0% 743 83645 9.5 U GPT2 SGDID:S000001775, Chr XI from 567560-569791, Verified ORF, "Glycerol-3-phosphate acyltransferase located in both lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone, which are intermediate steps in lipid biosynthesis" * Jamie_Mitco_SPB_95.34182.34182.2 1.8398 0.0751 92.3 1745.9122 1748.5817 5566.6 1 2.833 42.9 1 R.DGY*DVSSDAESSIS*R.M YKL079W 1 1 2.0% 656 73799 6.9 U SMY1 SGDID:S000001562, Chr XI from 286247-288217, Verified ORF, "Protein that interacts with Myo2p, proposed to be involved in exocytosis; N-terminal domain is related to the motor domain of kinesins" * Jamie_Mitco_SPB_55.19762.19762.2 1.7703 0.0357 88.6 1486.5721 1486.749 5821.9 46 3.019 54.2 1 R.EIQELLTTNAILK.G Reverse_YGR087C 1 1 2.0% 563 61580 6.2 U PDC6 SGDID:S000003319, Chr VII from 652985-651294, reverse complement, Verified ORF, "Minor isoform of pyruvate decarboxylase, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde, regulation is glucose- and ethanol-dependent, involved in amino acid catabolism" Reverse_YLR044C 1 1 2.0% 563 61495 6.2 U PDC1 SGDID:S000004034, Chr XII from 234082-232391, reverse complement, Verified ORF, "Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde; subject to glucose-, ethanol-, and autoregulation; involved in amino acid catabolism" Jamie_Mitco_SPB_21.07366.07366.2 1.5098 0.0162 82.3 1171.6522 1172.3262 6055.0 290 2.831 50.0 1 K.SLTGVYVGGYR.P YAL012W 1 1 2.0% 394 42542 6.5 U CYS3 SGDID:S000000010, Chr I from 130802-131986, Verified ORF, "Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine;" * Jamie_Mitco_SPB_7.02431.02431.2 1.8343 0.0276 91.8 803.27216 802.9059 4854.1 59 3.586 71.4 1 R.QAALSANK.I Reverse_YOL081W 2 2 1.9% 3079 351669 7.1 U IRA2 SGDID:S000005441, Chr XV from 171069-180308, Verified ORF, "GTPase-activating protein that negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, has similarity to Ira1p and human neurofibromin" * Jamie_Mitco_SPB_77.27493.27493.3 2.2769 0.0738 81.1 3840.7444 3841.3987 9689.2 187 2.897 16.1 1 K.VGLNVES*HQLASFMKTMQIIAKLYKPCY*APIK.K * Jamie_Mitco_SPB_70.25177.25177.2 1.5661 0.0105 80.9 3462.672 3464.6519 7488.5 84 2.47 16.7 1 K.SQHFFHKNGNS*HT*SSYDPLISNLKPRYK.Q YDR093W 2 2 1.9% 1612 182617 6.2 U DNF2 SGDID:S000002500, Chr IV from 631277-636115, Verified ORF, "Non-essential P-type ATPase that is a potential aminophospholipid translocase, localizes to the plasma membrane and late exocytic or early endocytic membranes, likely involved in protein transport; potential Cdc28p substrate" * Jamie_Mitco_SPB_85.30459.30459.2 1.7255 0.0806 92.1 1461.4722 1460.7698 4025.4 34 3.238 40.9 1 K.VRQSLKCSKIIK.S * Jamie_Mitco_SPB_74.26433.26433.2 2.0633 0.1641 99.4 1901.1322 1902.1118 5887.5 227 3.891 29.4 1 K.LDIKAQSPDESALVSTAR.Q YHR186C 2 2 1.9% 1557 177608 7.8 U KOG1 SGDID:S000001229, Chr VIII from 480672-475999, reverse complement, Verified ORF, "Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors" * Jamie_Mitco_SPB_34.12108.12108.2 1.7165 0.0061 83.3 1912.4521 1913.912 9639.0 318 2.533 32.1 1 R.S*FS*PNERVDNNAFKK.E * Jamie_Mitco_SPB_13.04523.04523.2 2.202 0.1239 98.9 1661.6322 1660.7812 5386.0 5 3.395 50.0 1 R.NRNETIIQETQGEK.K YKL101W 2 2 1.9% 1518 169592 9.3 U HSL1 SGDID:S000001584, Chr XI from 248564-253120, Verified ORF, "Nim1p-related protein kinase that regulates the morphogenesis and septin checkpoints; associates with the assembled septin filament; required along with Hsl7p for bud neck recruitment, phosphorylation, and degradation of Swe1p" * Jamie_Mitco_SPB_15.05215.05215.2 1.4833 0.0472 83.0 2065.0122 2062.274 5693.0 201 3.505 31.2 1 K.PMLSLPSSFLNT*S*MTFK.N * Jamie_Mitco_SPB_13.04546.04546.2 1.741 0.0256 87.9 1444.1721 1441.4514 6368.9 35 3.499 40.9 1 K.INT*S*PKASSFTK.E YDR011W 2 2 1.9% 1501 168766 7.6 U SNQ2 SGDID:S000002418, Chr IV from 465916-470421, Verified ORF, "ABC transporter protein involved in multidrug resistance and resistance to singlet oxygen species" Jamie_Mitco_SPB_8.02541.02541.1 1.0758 0.1083 95.5 870.52 869.86676 2868.8 26 3.407 57.1 1 R.GVS*GGERK.R * Jamie_Mitco_SPB_70.25079.25079.2 2.4152 0.2716 94.7 2276.4922 2274.6335 6888.0 16 4.371 27.5 1 R.MLLDNVSGYCIPGTMTALMGE.S Reverse_YBL085W 1 1 1.9% 980 109295 9.1 U BOI1 SGDID:S000000181, Chr II from 63873-66815, Verified ORF, "Protein implicated in polar growth, functionally redundant with Boi2p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain" * Jamie_Mitco_SPB_54.19128.19128.2 1.758 0.0413 88.1 2149.2322 2150.2183 6144.4 130 3.546 27.8 1 R.QLETTVSSS*KRDSSESSLK.N Reverse_YDR096W 1 1 1.9% 894 99481 7.5 U GIS1 SGDID:S000002503, Chr IV from 637134-639818, Verified ORF, "Transcriptional factor, involved in the expression of genes during nutrient limitation; also involved in the negative regulation of DPP1 and PHR1" * Jamie_Mitco_SPB_83.29832.29832.2 1.879 2.0E-4 85.6 2109.9321 2112.1125 6801.7 24 2.999 34.4 1 R.KGCRPCSHPKEGS*HVS*K.K Reverse_YOR018W 1 1 1.9% 837 92349 8.8 U ROD1 SGDID:S000005544, Chr XV from 364368-366881, Verified ORF, "Membrane protein; overexpression confers resistance to the GST substrate o-dinitrobenzene as well as to zinc and calcium; contains 2 PY motifs, which are required for Rod1p interaction with Rsp5p, a hect-type ubiquitin ligase" * Jamie_Mitco_SPB_122.43729.43729.2 1.5633 0.0915 89.4 1971.8522 1971.1404 9107.4 19 3.531 36.7 1 R.KHPGHVT*QLVT*PINLR.L Reverse_YFL023W 1 1 1.9% 796 91422 4.7 U BUD27 SGDID:S000001871, Chr VI from 90984-93374, Verified ORF, "Protein involved in bud-site selection, nutrient signaling, and gene expression controlled by the TOR kinase; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern" * Jamie_Mitco_SPB_104.37363.37363.2 1.6008 0.1154 92.1 1725.4321 1727.014 5055.9 113 3.62 39.3 1 K.KLIPKLSKNVDGEER.E YBR121C 1 1 1.9% 667 75411 6.0 U GRS1 SGDID:S000000325, Chr II from 483361-481358, reverse complement, Verified ORF, "Cytoplasmic and mitochondrial glycyl-tRNA synthase that ligates glycine to the cognate anticodon bearing tRNA; transcription termination factor that may interact with the 3'-end of pre-mRNA to promote 3'-end formation" * Jamie_Mitco_SPB_15.05362.05362.2 2.523 0.1733 100.0 1390.9321 1390.4436 6643.6 15 4.775 58.3 1 K.VDGVDGEVELDDK.L YHR124W 1 1 1.9% 627 71480 6.6 U NDT80 SGDID:S000001166, Chr VIII from 356564-358447, Verified ORF, "Meiosis-specific transcription factor required for exit from pachytene and for full meiotic recombination; activates middle sporulation genes; competes with Sum1p for binding to promoters containing middle sporulation elements (MSE)" * Jamie_Mitco_SPB_81.29032.29032.2 1.6568 0.1225 95.6 1590.5922 1589.8523 5581.7 343 3.637 40.9 1 K.MKKYDSTFYLHR.D YMR217W 1 1 1.9% 525 58482 6.5 U GUA1 SGDID:S000004830, Chr XIII from 701789-703366, Verified ORF, "GMP synthase, an enzyme that catalyzes the second step in the biosynthesis of GMP from inosine 5'-phosphate (IMP); transcription is not subject to regulation by guanine but is negatively regulated by nutrient starvation" * Jamie_Mitco_SPB_16.05523.05523.2 1.4019 0.0952 88.2 1069.7122 1072.2064 5387.1 46 3.591 55.6 1 R.IVNEVDGVAR.V YDL001W 1 1 1.9% 430 49932 5.7 U RMD1 SGDID:S000002159, Chr IV from 447981-449273, Verified ORF, "Cytoplasmic protein required for sporulation" * Jamie_Mitco_SPB_10.03551.03551.2 1.8065 0.0659 95.5 998.0122 998.17584 3590.6 7 3.058 71.4 1 R.KLHHTHPK.L Reverse_YNL262W 2 2 1.8% 2222 255669 7.0 U POL2 SGDID:S000005206, Chr XIV from 148213-154881, Verified ORF, "Catalytic subunit of DNA polymerase epsilon, one of the major chromosomal DNA replication polymerases characterized by processivity and proofreading exonuclease activity; also involved in DNA synthesis during DNA repair" * Jamie_Mitco_SPB_32.11476.11476.3 2.07 0.0725 81.6 1601.2444 1604.7783 3343.7 125 3.297 38.5 1 K.SRFT*VSAGLDMIAR.Q * Jamie_Mitco_SPB_98.35034.35034.3 2.6786 0.0369 81.4 3212.6943 3214.2446 5312.6 47 2.872 21.0 1 R.ACTKGPRNFDCS*ACDREAKIS*DPQLR.N Reverse_YLR024C 2 2 1.8% 1872 216777 6.3 U UBR2 SGDID:S000004014, Chr XII from 193282-187664, reverse complement, Verified ORF, "Cytoplasmic ubiquitin-protein ligase (E3)" * Jamie_Mitco_SPB_36.12849.12849.3 3.1879 0.2515 85.7 2641.7344 2643.867 7551.0 101 4.31 25.0 1 R.S*IES*SFTKSNVFKKYIFSKLE.C * Jamie_Mitco_SPB_34.12167.12167.2 1.9341 0.0791 96.2 1503.9321 1505.6677 6261.7 5 3.134 54.5 1 K.VKKAPVFT*DEYK.M YLR305C 1 1 1.8% 1900 214605 7.5 U STT4 SGDID:S000004296, Chr XII from 743865-738163, reverse complement, Verified ORF, "Phosphatidylinositol-4-kinase that functions in the Pkc1p protein kinase pathway; required for normal vacuole morphology, cell wall integrity, and actin cytoskeleton organization" * Jamie_Mitco_SPB_37.13302.13302.3 2.939 0.1796 98.1 4054.7644 4057.3767 9546.5 190 4.196 16.7 1 R.AHYLQTLCLIPFIEDTESELFESFTNVVS*ESIDK.F YJL113W 1 1 1.8% 1803 207708 8.0 U YJL113W SGDID:S000003649, Chr X from 197834-198919,198921-203246, transposable_element_gene, "TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition" * Jamie_Mitco_SPB_74.26493.26493.3 2.0109 0.1064 82.5 4067.6042 4065.8655 8134.2 157 3.067 16.4 1 K.SHQFS*SDNDDEEDQIETVT*NLCEALENYEDDNK.P YPL231W 1 1 1.8% 1887 206945 5.4 U FAS2 SGDID:S000006152, Chr XVI from 108652-114315, Verified ORF, "Alpha subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains beta-ketoacyl reductase and beta-ketoacyl synthase activities" * Jamie_Mitco_SPB_60.21546.21546.3 4.2117 0.2574 100.0 4058.6643 4059.4297 6366.1 8 5.282 19.7 1 R.EAQIKDWVENELEALKLEAEEIPSEDQNEFLLER.T YNL139C 2 2 1.8% 1597 183930 7.7 U RLR1 SGDID:S000005083, Chr XIV from 365719-360926, reverse complement, Verified ORF, "Subunit of the THO complex, which is required for efficient transcription elongation and involved in transcriptional elongation-associated recombination; required for LacZ RNA expression from certain plasmids" * Jamie_Mitco_SPB_77.27387.27387.2 1.9 0.0082 87.8 2035.3922 2033.3762 5280.8 48 2.733 29.4 1 R.IPT*LLSKISRIGVADIQK.N * Jamie_Mitco_SPB_30.10578.10578.2 1.8603 0.0468 92.3 1286.3522 1284.3315 6226.3 68 3.46 55.6 1 K.KNSS*QRSQLR.F YEL022W 2 2 1.8% 1459 165667 5.1 U GEA2 SGDID:S000000748, Chr V from 111421-115800, Verified ORF, "Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs), involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; similar to but not functionally redundant with Gea1p" * Jamie_Mitco_SPB_10.03310.03310.2 1.8988 0.032 94.7 894.9922 893.9749 4731.4 104 2.937 66.7 1 R.EEFGRQK.N * Jamie_Mitco_SPB_23.08067.08067.3 2.474 0.0572 84.7 2258.3342 2254.5027 7880.9 6 2.93 30.6 1 R.LKLSNLPKPSPEIS*INKS*K.E Reverse_YER008C 2 2 1.8% 1336 154694 5.4 U SEC3 SGDID:S000000810, Chr V from 171817-167807, reverse complement, Verified ORF, "Non-essential subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, Exo84p) which mediates targeting of post-Golgi vesicles to sites of active exocytosis; Sec3p specifically is a spatial landmark for secretion" * Jamie_Mitco_SPB_47.16857.16857.2 1.388 0.0173 80.2 1377.4521 1378.5283 4686.8 319 2.905 50.0 1 K.HTIS*RTAAISLK.R * Jamie_Mitco_SPB_13.04618.04618.2 2.2197 0.1227 99.2 1567.3722 1566.7948 6827.5 10 3.366 54.5 1 K.LLEDLT*NWLLKK.N Reverse_YOR227W 1 1 1.8% 1246 139457 8.9 U YOR227W SGDID:S000005753, Chr XV from 762825-766565, Uncharacterized ORF, "Hypothetical protein" * Jamie_Mitco_SPB_102.36603.36603.2 1.8495 0.0772 92.7 2568.4722 2570.6528 10314.6 75 3.45 23.8 1 K.KVSTTT*AESTRTSAASKT*RNRR.K YOR227W 1 1 1.8% 1246 139457 8.9 U YOR227W SGDID:S000005753, Chr XV from 762825-766565, Uncharacterized ORF, "Hypothetical protein" * Jamie_Mitco_SPB_112.40197.40197.2 1.03 0.1289 80.7 2799.172 2797.014 4902.5 196 3.739 15.9 1 R.VT*IAVQEFNSDPPQQLPS*RKPKR.G YKR096W 1 1 1.8% 1195 137491 5.4 U YKR096W SGDID:S000001804, Chr XI from 626435-630022, Uncharacterized ORF, "Hypothetical protein" * Jamie_Mitco_SPB_55.19456.19456.2 1.8831 0.1027 96.3 2429.3123 2431.3196 7116.0 28 3.658 30.0 1 K.ERKKSSNNDSSVTESST*GNS*R.N Reverse_YFR030W 1 1 1.8% 1035 114829 5.3 U MET10 SGDID:S000001926, Chr VI from 213300-216407, Verified ORF, "Subunit alpha of assimilatory sulfite reductase, which is responsible for the conversion of sulfite into sulfide" * Jamie_Mitco_SPB_25.08970.08970.2 1.621 0.0079 82.5 2333.8123 2333.5657 11254.0 176 2.725 30.6 1 R.KHRSGLYLFVEGIEY*GQQK.Q YOR005C 1 1 1.8% 944 108515 8.4 U DNL4 SGDID:S000005531, Chr XV from 337343-334509, reverse complement, Verified ORF, "DNA ligase required for nonhomologous end-joining (NHEJ), forms stable heterodimer with required cofactor Lif1p, catalyzes DNA ligation as part of a complex with Lif1p and Nej1p; involved in meiosis, not essential for vegetative growth" * Jamie_Mitco_SPB_40.14339.14339.2 2.0463 0.1728 99.4 2030.8922 2028.1893 7179.1 12 3.673 40.6 1 K.S*NPSYQAERSQLGLIRK.K YML065W 1 1 1.8% 914 104400 5.7 U ORC1 SGDID:S000004530, Chr XIII from 142210-144954, Verified ORF, "Largest subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; may be phosphorylated by Cdc28p" * Jamie_Mitco_SPB_59.21126.21126.2 1.7265 0.0548 88.9 1717.7322 1717.9756 5638.0 2 3.108 36.7 1 K.VASVSGDARRALKVCK.R Reverse_YER047C 1 1 1.8% 897 100332 9.0 U SAP1 SGDID:S000000849, Chr V from 246502-243809, reverse complement, Verified ORF, "Putative ATPase of the AAA family, interacts with the Sin1p transcriptional repressor in the two-hybrid system" * Jamie_Mitco_SPB_12.04183.04183.3 2.427 0.0409 82.3 1808.0944 1809.8496 5203.2 25 3.058 35.0 1 K.NQSAGHSYPKSNS*VPR.S Reverse_YER017C 2 2 1.8% 761 84544 9.2 U AFG3 SGDID:S000000819, Chr V from 191787-189502, reverse complement, Verified ORF, "Component, with Yta12p, of the mitochondrial inner membrane m-AAA protease that mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes" * Jamie_Mitco_SPB_68.24239.24239.2 1.6079 0.0454 87.9 1339.4922 1336.5344 4050.9 12 2.911 50.0 1 R.SRLVPKVT*SIR.S * Jamie_Mitco_SPB_35.12388.12388.2 1.4754 0.0925 88.8 1300.4521 1299.5999 5484.2 442 2.971 40.9 1 R.LVPKVTSIRSAK.G YKL182W 2 2 1.7% 2051 228689 5.9 U FAS1 SGDID:S000001665, Chr XI from 100676-106831, Verified ORF, "Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities" * Jamie_Mitco_SPB_29.10391.10391.2 2.2552 0.1083 98.7 2256.0723 2255.4058 8494.4 1 3.897 44.4 1 K.DSLWQSEHLEAVVDQDVQR.T * Jamie_Mitco_SPB_97.34615.34615.2 1.6218 0.0939 90.5 1907.5721 1908.9329 4679.5 263 3.17 35.7 1 K.EINEHST*SYTFRSEK.G Reverse_YER132C 2 2 1.7% 1753 195382 7.8 U PMD1 SGDID:S000000934, Chr V from 430445-425184, reverse complement, Verified ORF, "Protein with an N-terminal kelch-like domain, putative negative regulator of early meiotic gene expression; required, with Mds3p, for growth under alkaline conditions" * Jamie_Mitco_SPB_79.28235.28235.2 1.582 0.025 82.6 1952.8522 1951.0225 6068.2 162 2.865 35.7 1 R.QS*NPRIYKSFS*GHLR.S * Jamie_Mitco_SPB_89.31746.31746.2 1.9159 0.0345 90.0 1537.2322 1536.5065 4050.7 294 3.022 34.6 1 K.EAPSAKS*ISSPSS*K.A Reverse_YDR283C 1 1 1.7% 1659 190192 6.4 U GCN2 SGDID:S000002691, Chr IV from 1030043-1025064, reverse complement, Verified ORF, "Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex" * Jamie_Mitco_SPB_60.21551.21551.3 2.7375 0.0613 87.3 3244.2544 3244.3782 7960.3 241 2.94 19.4 1 K.HAEKSLNNYISTAFS*RPTSNNGS*GFVKR.L Reverse_YNL139C 1 1 1.7% 1597 183930 7.7 U RLR1 SGDID:S000005083, Chr XIV from 365719-360926, reverse complement, Verified ORF, "Subunit of the THO complex, which is required for efficient transcription elongation and involved in transcriptional elongation-associated recombination; required for LacZ RNA expression from certain plasmids" * Jamie_Mitco_SPB_47.16647.16647.3 3.3497 0.1154 95.1 3466.9143 3467.8513 4243.3 12 3.695 24.0 1 K.GKLCHKILEIFSWLLT*NMEDCRTS*LIK.Y YLR310C 1 1 1.7% 1589 179091 7.1 U CDC25 SGDID:S000004301, Chr XII from 756995-752226, reverse complement, Verified ORF, "Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1" * Jamie_Mitco_SPB_25.08650.08650.3 3.2684 0.0159 86.6 3220.9744 3218.6426 9264.8 103 2.737 20.2 1 K.KIFKGLISSLSRISINSHLY*FDSAFHR.K Reverse_YLL015W 1 1 1.7% 1559 176873 7.7 U BPT1 SGDID:S000003938, Chr XII from 116431-121110, Verified ORF, "ABC type transmembrane transporter of MRP/CFTR family, found in vacuolar membrane, involved in the transport of unconjugated bilirubin and in heavy metal detoxification via glutathione conjugates, along with Ycf1p" * Jamie_Mitco_SPB_63.22348.22348.3 3.0162 0.0165 84.0 3181.4644 3177.5115 9017.5 351 2.977 20.2 1 R.LVDVSMLNLIDGT*S*KENREALTLRLSK.Q YPL167C 2 2 1.7% 1504 172956 8.6 U REV3 SGDID:S000006088, Chr XVI from 237106-232592, reverse complement, Verified ORF, "Subunit of DNA polymerase zeta, which is involved in DNA repair; required for mutagenesis induced by DNA damage" * Jamie_Mitco_SPB_20.07015.07015.2 2.6008 0.1583 88.2 1706.8322 1706.6832 9379.7 4 4.298 50.0 1 E.Y*EICGVDNENEGVK.G * Jamie_Mitco_SPB_18.06222.06222.1 1.3921 0.0179 91.7 1564.36 1563.8839 3245.4 19 2.916 45.5 1 K.KLKRQKEYQTLK.T YJL095W 1 1 1.7% 1478 164195 9.5 U BCK1 SGDID:S000003631, Chr X from 247171-251607, Verified ORF, "Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p" * Jamie_Mitco_SPB_61.21949.21949.3 2.6153 0.0649 86.7 2741.7844 2741.71 8557.2 132 2.733 22.9 1 K.S*QEDISNSRSTSESALSPT*KSGPSK.T YDR464W 1 1 1.7% 1435 161596 8.9 U SPP41 SGDID:S000002872, Chr IV from 1388862-1393169, Verified ORF, "Protein involved in negative regulation of expression of spliceosome components PRP4 and PRP3" * Jamie_Mitco_SPB_75.26692.26692.2 1.828 0.0116 86.5 2585.8123 2586.6865 12480.0 104 2.885 23.9 1 K.TKSKTSKAASTANKSPASEST*S*KK.K Reverse_YBR081C 1 1 1.7% 1332 152617 4.9 U SPT7 SGDID:S000000285, Chr II from 405245-401247, reverse complement, Verified ORF, "Subunit of the SAGA transcriptional regulatory complex, involved in proper assembly of the complex; also present as a C-terminally truncated form in the SLIK/SALSA transcriptional regulatory complex" * Jamie_Mitco_SPB_22.07743.07743.4 2.8078 0.1055 80.8 2559.1365 2560.608 7542.6 26 3.027 28.6 1 K.GNEKIENKNVNEHVT*VTS*SATK.D Reverse_YDR097C 1 1 1.7% 1242 140080 5.8 U MSH6 SGDID:S000002504, Chr IV from 643832-640104, reverse complement, Verified ORF, "Protein required for mismatch repair in mitosis and meiosis, forms a complex with Msh2p to repair both single-base & insertion-deletion mispairs; potentially phosphorylated by Cdc28p" * Jamie_Mitco_SPB_50.17830.17830.4 3.0727 0.0937 83.7 2569.4966 2573.738 4116.0 54 3.133 27.5 1 K.NWAS*SPIYLTRPDYEPDSKPR.R YHR077C 1 1 1.7% 1089 126747 5.1 U NMD2 SGDID:S000001119, Chr VIII from 255758-255753,255639-252376, reverse complement, Verified ORF, "Protein involved in the nonsense-mediated mRNA decay (NMD) pathway; interacts with Nam7p and Upf3p" * Jamie_Mitco_SPB_117.41838.41838.2 1.977 0.2449 88.6 2233.2922 2230.486 5559.4 3 4.501 35.3 1 P.KKIAFT*FLTKSGKKTQS*R.I YBL085W 2 2 1.7% 980 109295 9.1 U BOI1 SGDID:S000000181, Chr II from 63873-66815, Verified ORF, "Protein implicated in polar growth, functionally redundant with Boi2p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain" * Jamie_Mitco_SPB_111.39756.39756.1 1.2627 0.03 91.7 961.14 960.887 3534.4 9 2.812 64.3 1 K.LS*SESSDR.K * Jamie_Mitco_SPB_21.07335.07335.3 1.9986 0.053 80.5 1233.8344 1238.1758 3431.7 232 3.102 40.6 1 R.RKS*SYGHS*R.D Reverse_YGR100W 1 1 1.7% 950 109259 5.4 U MDR1 SGDID:S000003332, Chr VII from 690249-693101, Verified ORF, "Cytoplasmic GTPase-activating protein for Ypt/Rab transport GTPases Ypt6p, Ypt31p and Sec4p; involved in recycling of internalized proteins and regulation of Golgi secretory function" * Jamie_Mitco_SPB_120.42907.42907.3 2.278 0.0494 80.2 2070.4443 2066.3647 6099.4 10 3.003 33.3 1 K.FAT*VLLEQFKTIQRYK.I Reverse_YLR409C 1 1 1.7% 939 104791 7.8 U UTP21 SGDID:S000004401, Chr XII from 937230-934411, reverse complement, Uncharacterized ORF, "Possible U3 snoRNP protein involved in maturation of pre-18S rRNA, based on computational analysis of large-scale protein-protein interaction data" * Jamie_Mitco_SPB_12.04097.04097.3 2.5821 0.2931 90.5 1728.7743 1728.8589 6420.5 53 5.122 31.7 1 P.QGNLFTAQTVGGYSER.H YKR021W 1 1 1.7% 915 102540 6.0 U YKR021W SGDID:S000001729, Chr XI from 478877-481624, Uncharacterized ORF, "Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm" * Jamie_Mitco_SPB_73.26230.26230.2 1.5005 0.0163 80.7 1726.7922 1723.973 7075.0 55 2.719 33.3 1 K.NRASLHATSVLPSTIR.Q Reverse_YDR520C 1 1 1.7% 772 88648 8.0 U YDR520C SGDID:S000002928, Chr IV from 1483393-1481075, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Mitco_SPB_25.08818.08818.2 1.5836 0.0847 89.5 1527.5721 1526.5198 6805.7 52 2.788 50.0 1 K.KNGNNKPSNT*ESR.E YOL036W 1 1 1.7% 761 84352 9.2 U YOL036W SGDID:S000005396, Chr XV from 256743-259028, Uncharacterized ORF, "Protein of unknown function; potential Cdc28p substrate" * Jamie_Mitco_SPB_45.15989.15989.2 1.566 0.0765 88.8 1513.4321 1510.4746 4970.9 166 2.862 41.7 1 R.SRSNS*SALAST*LR.D YAR035W 1 1 1.7% 687 77766 6.9 U YAT1 SGDID:S000000080, Chr I from 190187-192250, Verified ORF, "Outer mitochondrial carnitine acetyltransferase, minor ethanol-inducible enzyme involved in transport of activated acyl groups from the cytoplasm into the mitochondrial matrix" * Jamie_Mitco_SPB_17.05819.05819.2 1.8402 0.0843 94.8 1425.5721 1424.3318 3915.6 43 3.157 45.5 1 K.SLLDQDAS*DAT*K.I YPL269W 1 1 1.7% 644 74245 8.6 U KAR9 SGDID:S000006190, Chr XVI from 33013-34947, Verified ORF, "Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase" * Jamie_Mitco_SPB_68.24349.24349.2 1.8404 0.128 97.9 1490.6322 1488.6407 4144.8 33 3.728 55.0 1 K.Y*TKDITIRLER.Q Reverse_YDR266C 1 1 1.7% 639 72756 9.0 U YDR266C SGDID:S000002674, Chr IV from 1002017-1000098, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Mitco_SPB_10.03304.03304.2 2.0381 0.0381 93.7 1387.8121 1389.5052 2418.6 77 3.774 50.0 1 K.Y*SELLKEASLR.G Reverse_contaminant_KERATIN16 1 1 1.7% 534 57265 6.6 U no description * Jamie_Mitco_SPB_7.02277.02277.1 1.0542 0.0517 87.8 916.31 914.91644 3338.6 224 2.678 43.8 1 R.SGFGGSSCR.G Reverse_YDL171C 2 2 1.6% 2145 238100 6.6 U GLT1 SGDID:S000002330, Chr IV from 155641-149204, reverse complement, Verified ORF, "NAD(+)-dependent glutamate synthase (GOGAT), synthesizes glutamate from glutamine and alpha-ketoglutarate; with Gln1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source" * Jamie_Mitco_SPB_114.41039.41039.1 1.1763 0.0569 93.2 1096.27 1097.0961 7636.5 251 3.436 42.9 1 K.LEDMS*IT*K.G * Jamie_Mitco_SPB_46.16335.16335.3 2.28 0.0942 86.2 2764.9443 2761.1611 8199.0 34 2.996 23.0 1 R.NMAIALTSHAEMSISGYSMAGSAFRR.A Reverse_YDR141C 1 1 1.6% 1698 194686 5.7 U DOP1 SGDID:S000002548, Chr IV from 739992-734896, reverse complement, Verified ORF, "Protein of unknown function, essential for viability, involved in establishing cellular polarity and morphogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern" * Jamie_Mitco_SPB_56.20097.20097.4 3.065 0.1372 87.1 3187.1765 3189.682 6098.1 38 3.335 24.4 1 K.KDSWSIHSRGKLEESEPIKEFIAIFLK.S YOR290C 1 1 1.6% 1703 194050 7.0 U SNF2 SGDID:S000005816, Chr XV from 860255-855144, reverse complement, Verified ORF, "Catalytic subunit of the 11-subunit SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p" * Jamie_Mitco_SPB_90.32052.32052.3 2.3892 0.0964 88.6 3375.6243 3371.3357 8579.8 115 2.887 21.2 1 K.DEEYDDDDDNSNVDYY*NVAHRIKEDIK.K Reverse_YGL173C 1 1 1.6% 1528 175459 7.5 U KEM1 SGDID:S000003141, Chr VII from 180119-175533, reverse complement, Verified ORF, "5'-3' exonuclease involved in mRNA decay, evolutionarily conserved component of cytoplasmic processing (P) bodies, plays a role in microtubule-mediated processes, filamentous growth, and ribosomal RNA maturation" * Jamie_Mitco_SPB_95.33863.33863.4 3.4053 0.2036 96.6 2955.6165 2958.0813 4749.6 411 4.468 25.0 1 K.LRRDS*SY*GDITTEGGYAYDGLFIVK.S YOR151C 1 1 1.6% 1224 138751 6.5 U RPB2 SGDID:S000005677, Chr XV from 616671-612997, reverse complement, Verified ORF, "RNA polymerase II second largest subunit B150, part of central core; similar to bacterial beta subunit" * Jamie_Mitco_SPB_42.15021.15021.4 2.6368 0.1262 84.7 2222.5366 2224.4844 5353.5 88 3.227 31.5 1 R.YTYSSTLSHLRRTNTPIGR.D YFR016C 1 1 1.6% 1233 137698 4.5 U YFR016C SGDID:S000001912, Chr VI from 180735-177034, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Mitco_SPB_31.10947.10947.2 3.1721 0.1321 91.4 2286.4722 2287.4424 6540.4 1 3.922 42.1 1 S.YSTLATDLPLPS*TNDIIESR.D Reverse_YBR023C 1 1 1.6% 1165 131601 6.7 U CHS3 SGDID:S000000227, Chr II from 287925-284428, reverse complement, Verified ORF, "Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan" * Jamie_Mitco_SPB_34.11982.11982.3 2.4329 0.0659 85.6 2235.5044 2236.2708 7679.5 112 2.768 27.8 1 R.EHGNSLVLSLDY*GQLNS*TK.L YCL061C 1 1 1.6% 1096 124326 5.3 U MRC1 SGDID:S000000566, Chr III from 22106-18816, reverse complement, Verified ORF, "S-phase checkpoint protein found at replication forks, required for DNA replication; also required for Rad53p activation during DNA replication stress, where it forms a replication-pausing complex with Tof1p and is phosphorylated by Mec1p; protein involved in replication checkpoint" * Jamie_Mitco_SPB_70.24893.24893.2 1.4753 0.0041 80.0 1932.4122 1930.171 7556.4 310 2.998 31.2 1 K.HPSIIKSFASRTDINDK.F YBR079C 1 1 1.6% 964 110344 6.3 U RPG1 SGDID:S000000283, Chr II from 398271-395377, reverse complement, Verified ORF, "Subunit of the core complex of translation initiation factor 3(eIF3), essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes" * Jamie_Mitco_SPB_98.35247.35247.2 2.0852 0.234 87.3 1713.2922 1713.7985 4780.0 17 5.275 46.4 1 K.FKEHVSGTKES*ELAA.I YDR270W 1 1 1.6% 1004 109829 5.2 U CCC2 SGDID:S000002678, Chr IV from 1005669-1008683, Verified ORF, "Cu(+2)-transporting P-type ATPase, required for export of copper from the cytosol into an extracytosolic compartment; has similarity to human proteins involved in Menkes and Wilsons diseases" * Jamie_Mitco_SPB_5.01462.01462.3 2.7943 0.0943 93.4 1927.9143 1932.1993 7257.3 125 3.97 30.0 1 K.T*GTLTT*GFMVVKKFLK.D YHL007C 1 1 1.6% 939 102362 7.3 U STE20 SGDID:S000000999, Chr VIII from 97933-95114, reverse complement, Verified ORF, "Signal transducing kinase of the PAK (p21-activated kinase) family, involved in pheromone response and pseudohyphal/invasive growth pathways, activated by Cdc42p; binds Ste4p at a GBB motif present in noncatalytic domains of PAK kinases" * Jamie_Mitco_SPB_48.17161.17161.2 1.7755 0.1206 96.3 1710.7722 1708.8687 6804.7 251 3.375 35.7 1 K.SFPS*KNPLKNSSPPK.K Reverse_YOR211C 1 1 1.6% 902 101523 8.0 U MGM1 SGDID:S000005737, Chr XV from 741632-738924, reverse complement, Verified ORF, "Mitochondrial GTPase related to dynamin, present in a complex containing Ugo1p and Fzo1p; required for normal morphology of cristae and for stability of Tim11p; homolog of human OPA1 involved in autosomal dominant optic atrophy" * Jamie_Mitco_SPB_29.10326.10326.2 2.0687 0.0366 92.6 1695.1721 1695.9745 7070.8 151 3.336 38.5 1 K.NFKEKMSEGLDKIR.D YER162C 1 1 1.6% 754 87204 7.8 U RAD4 SGDID:S000000964, Chr V from 502889-500625, reverse complement, Verified ORF, "Protein that recognizes and binds damaged DNA (with Rad23p) during nucleotide excision repair; subunit of Nuclear Excision Repair Factor 2 (NEF2); homolog of human XPC protein" * Jamie_Mitco_SPB_36.12717.12717.2 1.7247 0.0851 92.7 1311.8522 1310.4062 4362.4 239 3.106 45.5 1 K.IDTSLNGNNAYK.D Reverse_YOR034C 1 1 1.6% 749 85236 6.7 U AKR2 SGDID:S000005560, Chr XV from 397085-394836, reverse complement, Verified ORF, "Ankyrin repeat-containing protein similar to Akr1p; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; possibly involved in constitutive endocytosis of Ste3p" * Jamie_Mitco_SPB_65.23079.23079.2 1.4135 0.0646 84.3 1424.0721 1425.6653 6951.9 28 3.219 50.0 1 K.ILIFLEPT*TIGK.L YPL158C 1 1 1.6% 758 84846 6.7 U YPL158C SGDID:S000006079, Chr XVI from 254309-252033, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Mitco_SPB_44.15538.15538.2 1.584 0.1028 91.7 1230.3322 1229.4221 3865.7 403 3.127 54.5 1 R.SKPSTALNAIAR.Q Reverse_YLR289W 1 1 1.6% 645 73189 9.2 U GUF1 SGDID:S000004280, Chr XII from 715091-717028, Verified ORF, "Mitochondrial GTPase of unknown function, similar to E. coli elongation factor-type GTP-binding protein LepA and to LK1236.1 from Caenorhabditis elegans" * Jamie_Mitco_SPB_10.03513.03513.3 3.103 0.0502 93.7 1204.1044 1201.4056 4593.2 119 3.291 52.8 1 R.EVELKDLVQK.N Reverse_YKL204W 1 1 1.6% 632 69762 9.5 U EAP1 SGDID:S000001687, Chr XI from 53705-55603, Verified ORF, "eIF4E-associated protein, binds eIF4E and inhibits cap-dependent translation, also functions independently of eIF4E to maintain genetic stability; plays a role in cell growth, implicated in the TOR signaling cascade" * Jamie_Mitco_SPB_26.09262.09262.2 1.7515 0.0569 91.0 1314.4922 1315.4751 5717.2 15 2.992 50.0 1 K.NQRLQRDEKK.N YFL055W 1 2 1.6% 558 61052 7.5 U AGP3 SGDID:S000001839, Chr VI from 17004-18680, Uncharacterized ORF, "Low-affinity amino acid permease, may act to supply the cell with amino acids as nitrogen source in nitrogen-poor conditions; transcription is induced under conditions of sulfur limitation" * Jamie_Mitco_SPB_18.06429.06429.2 1.8848 0.0259 91.6 1058.5721 1060.1552 5690.2 117 3.167 62.5 2 R.SINLDEGRR.K YOR326W 1 1 1.5% 1574 180679 6.3 U MYO2 SGDID:S000005853, Chr XV from 925716-930440, Verified ORF, "One of two type V myosins, involved in polarized distribution of mitochondria; required for mitochondrion and vacuole inheritance and nuclear spindle orientation; moves multiple cargo" * Jamie_Mitco_SPB_39.13787.13787.4 3.0195 0.09 80.4 2726.1365 2725.9263 7660.2 396 3.147 24.6 1 K.LGILSLLDEESRLPAGSDESWT*QK.L Reverse_YGL131C 1 1 1.5% 1403 163203 8.6 U SNT2 SGDID:S000003099, Chr VII from 265864-261653, reverse complement, Verified ORF, "DNA binding protein with similarity to the S. pombe Snt2 protein" * Jamie_Mitco_SPB_28.09738.09738.2 1.3024 0.0771 82.6 2413.5723 2414.6096 7528.0 251 2.836 27.5 1 K.ENVGTQMRIKIKEDDSGGT*VR.Y YDR293C 1 1 1.5% 1250 139954 7.6 U SSD1 SGDID:S000002701, Chr IV from 1049386-1045634, reverse complement, Verified ORF, "Protein with a role in maintenance of cellular integrity, interacts with components of the TOR pathway; ssd1 mutant of a clinical S. cerevisiae strain displays elevated virulence" * Jamie_Mitco_SPB_20.07107.07107.3 3.5321 0.0344 93.0 2327.5745 2325.4966 9124.3 180 3.206 30.6 1 R.DNNFLSNEYLDQKNPQKEK.P Reverse_YFR016C 1 1 1.5% 1233 137698 4.5 U YFR016C SGDID:S000001912, Chr VI from 180735-177034, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Mitco_SPB_121.43283.43283.4 2.6603 0.167 89.8 2043.8566 2043.2847 6150.3 197 3.786 27.8 1 K.GIETEKQNKALVSPSIGDR.D YKR086W 1 1 1.5% 1071 121652 8.2 U PRP16 SGDID:S000001794, Chr XI from 599499-602714, Verified ORF, "RNA helicase in the DEAH-box family involved in the second catalytic step of splicing, exhibits ATP-dependent RNA unwinding activity" * Jamie_Mitco_SPB_65.23273.23273.2 1.7237 0.0839 91.0 1809.8121 1810.0984 5928.9 59 3.738 40.0 1 R.KIIIAT*NIAETSLTIK.G YHR027C 1 1 1.5% 993 109492 4.6 U RPN1 SGDID:S000001069, Chr VIII from 164704-161723, reverse complement, Verified ORF, "Non-ATPase base subunit of the 19S regulatory particle of the 26S proteasome; may participate in the recognition of several ligands of the proteasome; contains a leucine-rich repeat (LRR) domain, a site for protein?protein interactions" * Jamie_Mitco_SPB_12.04144.04144.2 2.5367 0.0682 98.6 1497.6921 1497.6945 5072.2 83 3.556 46.4 1 R.VGQAVETVGQAGRPK.K YFR013W 1 1 1.5% 787 90897 7.8 U IOC3 SGDID:S000001909, Chr VI from 169914-172277, Verified ORF, "Member of a complex (Isw1a) with Isw1p that has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; has homology to Esc8p, which is involved in silencing" * Jamie_Mitco_SPB_76.27359.27359.2 1.6246 0.1168 93.4 1402.3121 1399.6334 4023.2 9 3.402 45.5 1 K.QLKEGKKPDLSR.K Reverse_YNL186W 1 1 1.5% 792 88517 5.5 U UBP10 SGDID:S000005130, Chr XIV from 289500-291878, Verified ORF, "Ubiquitin-specific protease that deubiquitinates ubiquitin-protein moieties; may regulate silencing by acting on Sir4p; involved in posttranscriptionally regulating Gap1p and possibly other transporters; primarily located in the nucleus" * Jamie_Mitco_SPB_22.07765.07765.1 1.0456 0.0877 93.9 1176.49 1177.2133 5256.0 96 2.964 40.9 1 K.AGTADAADKDNK.S YDR211W 1 1 1.5% 712 81161 4.8 U GCD6 SGDID:S000002619, Chr IV from 884722-886860, Verified ORF, "Catalytic epsilon subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression" * Jamie_Mitco_SPB_22.07693.07693.2 1.546 0.0664 88.2 1203.2122 1204.4087 5738.7 117 3.05 50.0 1 R.TIEPAAFVLDK.S YML007W 1 1 1.5% 650 72533 5.0 U YAP1 SGDID:S000004466, Chr XIII from 253848-255800, Verified ORF, "Basic leucine zipper (bZIP) transcription factor required for oxidative stress tolerance; mediates pleiotropic drug and metal resistance; localized to the nucleus in response to the presence of oxidants" * Jamie_Mitco_SPB_9.02891.02891.2 1.3639 0.0526 82.6 1164.3322 1164.2194 5830.0 249 3.225 50.0 1 K.YENSFSGFGR.L YDL171C 2 2 1.4% 2145 238100 6.6 U GLT1 SGDID:S000002330, Chr IV from 155641-149204, reverse complement, Verified ORF, "NAD(+)-dependent glutamate synthase (GOGAT), synthesizes glutamate from glutamine and alpha-ketoglutarate; with Gln1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source" * Jamie_Mitco_SPB_8.02871.02871.1 1.031 0.0311 83.3 759.75 760.909 4245.9 20 2.814 58.3 1 K.VSKDIAK.T * Jamie_Mitco_SPB_47.16869.16869.2 1.6898 5.0E-4 82.6 2424.912 2425.5554 8613.6 4 2.592 25.0 1 R.T*GFT*VGVIGSGPAGLACADMLNR.A Reverse_YJR033C 1 1 1.4% 1357 154932 8.2 U RAV1 SGDID:S000003794, Chr X from 496365-492292, reverse complement, Verified ORF, "Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate" * Jamie_Mitco_SPB_80.28546.28546.2 1.7766 0.0292 87.8 2144.5723 2144.296 8200.3 187 3.079 27.8 1 R.MRATSIPES*AMAGIASY*AR.D YCR073C 1 1 1.4% 1331 152717 7.7 U SSK22 SGDID:S000000669, Chr III from 246579-242584, reverse complement, Verified ORF, "MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; functionally redundant with, and homologous to, Ssk2p; interacts with and is activated by Ssk1p; phosphorylates Pbs2p" * Jamie_Mitco_SPB_63.22637.22637.2 2.2192 0.2173 88.4 1950.1921 1952.2197 6511.3 159 4.46 32.4 1 M.MGT*PMYMAPETISGSAVK.G YNL250W 2 2 1.4% 1312 152568 6.1 U RAD50 SGDID:S000005194, Chr XIV from 175411-179349, Verified ORF, "Subunit of MRX complex, with Mre11p and Xrs2p, involved in processing double-strand DNA breaks in vegetative cells, initiation of meiotic DSBs, telomere maintenance, and nonhomologous end joining" * Jamie_Mitco_SPB_12.04127.04127.2 1.9266 0.0316 93.7 948.3122 947.033 5188.0 61 2.479 64.3 1 K.ELAESEIR.P * Jamie_Mitco_SPB_15.05151.05151.2 3.1376 0.0509 99.8 1163.8121 1163.27 3325.0 64 3.846 66.7 1 K.ELKDLENSSK.T Reverse_YNL091W 2 5 1.4% 1240 141514 5.4 U NST1 SGDID:S000005035, Chr XIV from 452410-456132, Verified ORF, "Protein of unknown function, mediates sensitivity to salt stress; interacts physically with the splicing factor Msl1p and also displays genetic interaction with MSL1" * Jamie_Mitco_SPB_21.07348.07348.2 1.4365 0.1209 91.8 934.03217 933.9132 4191.1 53 3.736 64.3 1 K.SHGAPT*QR.A * Jamie_Mitco_SPB_14.04965.04965.3 3.0705 0.0895 97.7 1216.7943 1217.3677 6872.0 43 3.369 50.0 4 K.KLREKEEER.K YBL007C 1 1 1.4% 1244 135848 5.9 U SLA1 SGDID:S000000103, Chr II from 216369-212635, reverse complement, Verified ORF, "Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; contains 3 SH3 domains; interacts with proteins regulating actin dynamics and with proteins required for endocytosis" * Jamie_Mitco_SPB_34.11939.11939.2 2.1504 0.0143 90.8 2078.412 2078.0305 5195.9 52 2.802 35.3 1 K.DDELQNDVVGS*AAGKRS*R.K YIL031W 1 1 1.4% 1034 116882 6.8 U ULP2 SGDID:S000001293, Chr IX from 292632-295736, Verified ORF, "Peptidase that deconjugates Smt3/SUMO-1 peptides from proteins, plays a role in chromosome cohesion at centromeric regions and recovery from checkpoint arrest induced by DNA damage or DNA replication defects; potential Cdc28p substrate" * Jamie_Mitco_SPB_10.03463.03463.3 2.3764 0.0303 80.4 1673.2743 1672.7924 5908.1 101 3.0 38.5 1 K.FNSLKPLDTLNSS*R.A YIL137C 1 1 1.4% 946 107723 6.5 U YIL137C SGDID:S000001399, Chr IX from 92788-89948, reverse complement, Uncharacterized ORF, "Putative metalloprotease" * Jamie_Mitco_SPB_101.36328.36328.1 1.089 0.0633 91.6 1567.3 1567.5723 5984.2 212 3.297 29.2 1 K.GTT*T*GDHLHKALR.S YOR211C 1 1 1.4% 902 101523 8.0 U MGM1 SGDID:S000005737, Chr XV from 741632-738924, reverse complement, Verified ORF, "Mitochondrial GTPase related to dynamin, present in a complex containing Ugo1p and Fzo1p; required for normal morphology of cristae and for stability of Tim11p; homolog of human OPA1 involved in autosomal dominant optic atrophy" * Jamie_Mitco_SPB_47.16605.16605.2 1.5921 0.0503 87.1 1628.8121 1625.8674 7102.1 8 2.564 50.0 1 K.QIVSHQFEKAYFK.E Reverse_YBL023C 1 1 1.4% 868 98780 5.1 U MCM2 SGDID:S000000119, Chr II from 177529-174923, reverse complement, Verified ORF, "Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex" * Jamie_Mitco_SPB_22.07831.07831.2 1.6023 0.025 84.4 1388.2522 1390.5333 8273.8 399 2.831 40.9 1 K.VNSLSELTLEER.L YML061C 1 1 1.4% 859 97682 9.0 U PIF1 SGDID:S000004526, Chr XIII from 151532-148953, reverse complement, Verified ORF, "DNA helicase involved in telomere formation and elongation; acts as a catalytic inhibitor of telomerase; also plays a role in repair and recombination of mitochondrial DNA" * Jamie_Mitco_SPB_20.07097.07097.2 2.0399 0.0971 97.4 1470.9922 1472.6847 6593.2 27 3.491 50.0 1 K.QLEADEQVKKRK.L YPL084W 1 1 1.4% 844 97276 5.4 U BRO1 SGDID:S000006005, Chr XVI from 394035-396569, Verified ORF, "Cytoplasmic class E vacuolar protein sorting (VPS) factor that coordinates deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes" * Jamie_Mitco_SPB_44.15695.15695.2 1.6791 0.0907 92.2 1286.0922 1288.2676 4744.2 55 3.279 50.0 1 K.SSLVAASNS*DEK.L YER069W 1 1 1.4% 863 94869 8.3 U ARG5,6 SGDID:S000000871, Chr V from 295408-297999, Verified ORF, "Protein that is processed in the mitochondrion to yield acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase, which catalyze the 2nd and 3rd steps in arginine biosynthesis; enzymes form a complex with Arg2p" * Jamie_Mitco_SPB_39.13847.13847.2 1.7923 0.0544 90.8 1352.9122 1350.6444 5552.2 17 3.267 50.0 1 K.YKLVGNIKSVTK.E YKL103C 1 1 1.4% 514 57093 5.8 U LAP4 SGDID:S000001586, Chr XI from 247326-245782, reverse complement, Verified ORF, "Vacuolar aminopeptidase, often used as a marker protein in studies of autophagy and cytosol to vacuole targeting (CVT) pathway" * Jamie_Mitco_SPB_117.41920.41920.1 1.1348 0.0214 85.4 855.98 856.95416 4554.0 11 2.981 66.7 1 K.KS*PLFGK.H YNL271C 2 2 1.3% 1953 219701 6.5 U BNI1 SGDID:S000005215, Chr XIV from 135384-129523, reverse complement, Verified ORF, "Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1" * Jamie_Mitco_SPB_96.34263.34263.2 1.8244 0.2611 86.5 1607.8121 1609.474 4195.9 4 4.41 39.3 1 R.LANSNATNSNTGS*PT*.Y * Jamie_Mitco_SPB_121.43353.43353.2 1.9179 0.0339 91.5 1205.1522 1206.3011 7637.7 88 3.635 55.0 1 R.NSSQASNKLTR.Q YOR341W 1 1 1.3% 1664 186431 7.1 U RPA190 SGDID:S000005868, Chr XV from 960982-965976, Verified ORF, "RNA polymerase I subunit; largest subunit of RNA polymerase I" * Jamie_Mitco_SPB_51.18093.18093.2 1.6132 0.0389 85.2 2449.2922 2450.6428 7101.1 30 3.028 30.0 1 K.RSEYVDMAIAKALSDGRTT*ER.G YLL015W 1 1 1.3% 1559 176873 7.7 U BPT1 SGDID:S000003938, Chr XII from 116431-121110, Verified ORF, "ABC type transmembrane transporter of MRP/CFTR family, found in vacuolar membrane, involved in the transport of unconjugated bilirubin and in heavy metal detoxification via glutathione conjugates, along with Ycf1p" * Jamie_Mitco_SPB_90.32141.32141.2 1.9885 0.2375 87.5 2173.652 2176.2122 5891.0 131 4.135 28.9 1 R.GDDSNEEDGNVNDILDVKIN.E YER093C 1 1 1.3% 1430 164368 8.7 U TSC11 SGDID:S000000895, Chr V from 347608-343316, reverse complement, Verified ORF, "Subunit of TORC2 (Tor2p-Lst8p-Avo1-Avo2-Tsc11p-Bit61p), a membrane-associated complex that regulates actin cytoskeletal dynamics during polarized growth and cell wall integrity; involved in sphingolipid metabolism; contains a RasGEFN domain" * Jamie_Mitco_SPB_78.28078.28078.2 1.6619 0.044 87.1 2277.8123 2277.2334 6620.8 171 3.056 27.8 1 R.DST*ISDIYSGSYST*NHLQK.H Reverse_YAL017W 1 1 1.3% 1356 152330 5.7 U PSK1 SGDID:S000000015, Chr I from 120226-124296, Verified ORF, "One of two (see also PSK2) PAS domain containing S/T protein kinases; coordinately regulates protein synthesis and carbohydrate metabolism and storage in response to a unknown metabolite that reflects nutritional status" * Jamie_Mitco_SPB_70.24993.24993.2 1.8802 0.1851 98.8 1874.0322 1874.9536 6678.8 7 3.707 32.4 1 K.GSSKSDALSETSPSLS*IK.S YAL017W 1 1 1.3% 1356 152330 5.7 U PSK1 SGDID:S000000015, Chr I from 120226-124296, Verified ORF, "One of two (see also PSK2) PAS domain containing S/T protein kinases; coordinately regulates protein synthesis and carbohydrate metabolism and storage in response to a unknown metabolite that reflects nutritional status" * Jamie_Mitco_SPB_88.31633.31633.2 1.717 0.033 86.9 1888.2922 1886.17 5411.5 8 2.977 37.5 1 R.NGVVFDPDDDLLPMPIK.V YPL137C 1 1 1.3% 1276 140891 7.4 U YPL137C SGDID:S000006058, Chr XVI from 296646-292816, reverse complement, Uncharacterized ORF, "Glc7-interacting protein whose overexpression relocalizes Glc7p from the nucleus" * Jamie_Mitco_SPB_38.13439.13439.2 2.2237 0.2101 88.5 2008.6721 2009.2296 7278.3 1 4.643 37.5 1 T.SGEKTKRS*IFGSLFSKR.S YDR097C 1 1 1.3% 1242 140080 5.8 U MSH6 SGDID:S000002504, Chr IV from 643832-640104, reverse complement, Verified ORF, "Protein required for mismatch repair in mitosis and meiosis, forms a complex with Msh2p to repair both single-base & insertion-deletion mispairs; potentially phosphorylated by Cdc28p" * Jamie_Mitco_SPB_50.17908.17908.2 1.8077 0.0045 85.2 1952.4521 1955.1962 7895.9 379 2.991 30.0 1 K.GT*LFKLFNRAIT*PMGK.R Reverse_YNL087W 1 1 1.3% 1178 132509 8.5 U TCB2 SGDID:S000005031, Chr XIV from 462413-465949, Verified ORF, "Bud-specific protein with a potential role in membrane trafficking; GFP-fusion protein migrates from the cell surface to intracellular vesicles near vacuole; contains 3 calcium and lipid binding domains; mRNA is targeted to the bud via the mRNA transport system involving She2p" * Jamie_Mitco_SPB_10.03439.03439.3 2.0743 0.1391 91.9 1878.6543 1882.9823 5219.9 114 3.291 32.1 1 K.EVT*QEKQALDRLSER.Y YFL034W 1 1 1.3% 1073 119496 5.2 U YFL034W SGDID:S000001860, Chr VI from 65475-68696, Uncharacterized ORF, "Possible integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk" * Jamie_Mitco_SPB_15.05292.05292.2 2.5016 0.0696 98.7 1497.9122 1497.7812 5956.6 50 2.806 50.0 1 K.ILADTLIARNLGAR.P Reverse_YBL104C 1 1 1.3% 1038 117636 6.1 U YBL104C SGDID:S000000200, Chr II from 21293-18177, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Mitco_SPB_80.28673.28673.2 1.7526 0.103 94.5 1473.8722 1476.6726 4841.5 191 3.505 37.5 1 K.Y*ALYGAIATAILR.L Reverse_YER114C 1 1 1.3% 1040 115688 8.8 U BOI2 SGDID:S000000916, Chr V from 393708-390586, reverse complement, Verified ORF, "Protein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain" * Jamie_Mitco_SPB_55.19545.19545.2 1.7454 0.0601 89.7 1609.5922 1610.7244 5843.2 307 2.954 38.5 1 K.SKSSLSSSHRT*PKK.K YLR309C 1 1 1.3% 911 105225 5.6 U IMH1 SGDID:S000004300, Chr XII from 751771-749036, reverse complement, Verified ORF, "Protein involved in vesicular transport, mediates transport between an endosomal compartment and the Golgi, contains a Golgi-localization (GRIP) domain that interacts with activated Arl1p-GTP to localize Imh1p to the Golgi" * Jamie_Mitco_SPB_19.06639.06639.2 2.0568 0.0092 90.9 1319.9122 1318.4668 7113.5 1 2.796 63.6 1 R.EEGLKSEVDALK.H YPL105C 1 1 1.3% 849 94356 7.8 U YPL105C SGDID:S000006026, Chr XVI from 355409-352860, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Mitco_SPB_20.07020.07020.2 1.9352 0.0486 93.2 1497.4722 1496.7068 5715.1 37 3.198 55.0 1 K.LWEQVQKKT*KK.F YKL204W 1 1 1.3% 632 69762 9.5 U EAP1 SGDID:S000001687, Chr XI from 53705-55603, Verified ORF, "eIF4E-associated protein, binds eIF4E and inhibits cap-dependent translation, also functions independently of eIF4E to maintain genetic stability; plays a role in cell growth, implicated in the TOR signaling cascade" * Jamie_Mitco_SPB_114.40745.40745.1 1.4751 0.0334 94.9 988.36 989.03595 2919.6 13 3.382 57.1 1 K.NGKNDERR.M Reverse_YHL027W 1 1 1.3% 625 68232 7.2 U RIM101 SGDID:S000001019, Chr VIII from 51109-52986, Verified ORF, "Transcriptional repressor involved in the response to pH; required for alkaline pH-stimulated differentiation pathways such as haploid invasive growth and sporulation; activated by proteolytic processing; has similarity to the A. nidulans transcription factor PacC" * Jamie_Mitco_SPB_9.03171.03171.2 1.9019 0.037 93.8 942.89215 942.20776 3847.0 174 3.057 64.3 1 R.RSPLMPIK.S YOR132W 1 1 1.3% 551 63204 6.9 U VPS17 SGDID:S000005658, Chr XV from 573175-574830, Verified ORF, "Subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde protein transport; peripheral membrane protein that assembles onto the membrane with Vps5p to promote vesicle formation" * Jamie_Mitco_SPB_5.01737.01737.1 1.2053 0.1084 96.2 916.73 917.0501 2115.5 2 4.227 66.7 1 K.KYT*LLAK.V YBR140C 1 1 1.2% 3092 350980 6.6 U IRA1 SGDID:S000000344, Chr II from 526622-517344, reverse complement, Verified ORF, "GTPase-activating protein that negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase" * Jamie_Mitco_SPB_37.13307.13307.3 2.0767 0.2054 93.8 4371.0244 4368.7593 5871.8 92 3.504 17.1 1 R.NDNY*KPGFSSHRPNFHTSRPKHTRINPALAT*MLLCK.I Reverse_YDR150W 2 2 1.2% 2748 313032 5.4 U NUM1 SGDID:S000002557, Chr IV from 755623-763869, Verified ORF, "Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex" * Jamie_Mitco_SPB_48.17148.17148.2 1.6171 0.0496 87.2 1492.9722 1495.7155 5322.7 165 2.775 45.8 1 K.INEYAPIFITADK.V * Jamie_Mitco_SPB_19.06789.06789.3 2.4893 0.0968 90.8 2174.3342 2173.298 6635.1 167 3.475 29.2 1 R.QLESYEESS*VVTHGLKASK.E Reverse_YDL058W 1 1 1.2% 1790 206451 4.9 U USO1 SGDID:S000002216, Chr IV from 345665-351037, Verified ORF, "Essential protein involved in intracellular protein transport, coiled-coil protein necessary for transport from ER to Golgi; required for assembly of the ER-to-Golgi SNARE complex" * Jamie_Mitco_SPB_86.30927.30927.2 1.813 0.3217 90.0 2669.4321 2666.8645 5643.1 112 4.652 22.5 1 K.LKET*LKAVLNDHSQFRSKY*EV.L Reverse_YNL250W 1 1 1.2% 1312 152568 6.1 U RAD50 SGDID:S000005194, Chr XIV from 175411-179349, Verified ORF, "Subunit of MRX complex, with Mre11p and Xrs2p, involved in processing double-strand DNA breaks in vegetative cells, initiation of meiotic DSBs, telomere maintenance, and nonhomologous end joining" * Jamie_Mitco_SPB_16.05590.05590.2 2.0769 0.0604 94.6 1829.2122 1828.9738 6080.6 21 2.756 40.0 1 K.LNELEHNLSDQTSLSK.F YDL019C 1 1 1.2% 1283 145796 6.8 U OSH2 SGDID:S000002177, Chr IV from 421511-417660, reverse complement, Verified ORF, "Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability" * Jamie_Mitco_SPB_32.11322.11322.2 2.2965 0.2189 90.5 1790.1322 1789.8505 9940.9 55 5.035 35.7 1 S.ELLDQAAT*FEDSTLR.T Reverse_YLR272C 1 1 1.2% 1176 132967 5.6 U YCS4 SGDID:S000004262, Chr XII from 687204-683674, reverse complement, Verified ORF, "Non-SMC subunit of the condensin complex (Smc2p-Smc4p-Ycs4p-Brn1p-Ycg1p); required for establishment and maintenance of chromosome condensation, chromosome segregation, chromatin binding of condensin and silencing at the mating type locus" * Jamie_Mitco_SPB_11.03708.03708.2 2.5746 0.297 80.0 1699.2922 1698.8247 4508.6 1 5.513 57.7 1 E.ITDNLT*EQSEVKKL.T Reverse_YER111C 1 1 1.2% 1093 123806 8.8 U SWI4 SGDID:S000000913, Chr V from 385872-382591, reverse complement, Verified ORF, "DNA binding component of the SBF complex (Swi4p-Swi6p), a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair" * Jamie_Mitco_SPB_36.12819.12819.3 1.9917 0.088 84.5 1638.5643 1636.8479 3441.5 142 3.074 37.5 1 K.FVQTINIWDDKTR.R YHR082C 1 1 1.2% 1029 117082 6.6 U KSP1 SGDID:S000001124, Chr VIII from 271550-268461, reverse complement, Verified ORF, "Nonessential putative serine/threonine protein kinase of unknown cellular role; overproduction causes allele-specific suppression of the prp20-10 mutation" * Jamie_Mitco_SPB_14.05044.05044.2 2.3018 0.2851 100.0 1451.6122 1450.5997 3998.4 1 5.183 63.6 1 R.NHHVSPTNQFLR.V Reverse_YNL014W 1 1 1.2% 1044 115868 6.2 U HEF3 SGDID:S000004959, Chr XIV from 606320-609454, Verified ORF, "Translational elongation factor EF-3; paralog of YEF3 and member of the ABC superfamily; stimulates EF-1 alpha-dependent binding of aminoacyl-tRNA by the ribosome; normally expressed in zinc deficient cells" * Jamie_Mitco_SPB_25.08947.08947.1 1.2521 0.1435 98.6 1473.58 1474.6715 3658.8 8 3.709 37.5 1 K.TTGPYQFTMNSVK.V YGL241W 1 1 1.2% 1004 113921 4.7 U KAP114 SGDID:S000003210, Chr VII from 45445-48459, Verified ORF, "Karyopherin, responsible for nuclear import of Spt15p, histones H2A and H2B, and Nap1p; amino terminus shows similarity to those of other importins, particularly Cse1p; localization is primarily nuclear" * Jamie_Mitco_SPB_122.43707.43707.2 1.4361 0.0694 85.4 1289.2322 1290.4998 8956.1 442 3.335 45.5 1 K.VLNTETSTLIAK.I Reverse_YDL223C 1 1 1.2% 1046 113616 6.4 U HBT1 SGDID:S000002382, Chr IV from 60406-57266, reverse complement, Verified ORF, "Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis" * Jamie_Mitco_SPB_21.07407.07407.2 2.1758 0.1917 84.0 1487.3322 1487.5658 6027.5 112 3.962 50.0 1 T.NKSDDFPNSSFTK.S Reverse_YGR162W 1 1 1.2% 952 107101 6.0 U TIF4631 SGDID:S000003394, Chr VII from 824064-826922, Verified ORF, "Translation initiation factor eIF4G, subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); associates with the poly(A)-binding protein Pab1p, also interacts with eIF4A (Tif1p); homologous to Tif4632p" * Jamie_Mitco_SPB_33.11711.11711.2 1.8676 0.0642 93.3 1398.7722 1399.4515 4290.8 128 2.915 45.0 1 R.EVEDKDLLET*K.G Reverse_YCR057C 1 1 1.2% 923 103983 5.1 U PWP2 SGDID:S000000653, Chr III from 223223-220452, reverse complement, Verified ORF, "Conserved 90S pre-ribosomal component essential for proper endonucleolytic cleavage of the 35 S rRNA precursor at A0, A1, and A2 sites; contains eight WD-repeats; PWP2 deletion leads to defects in cell cycle and bud morphogenesis" * Jamie_Mitco_SPB_51.18035.18035.2 1.5352 0.025 83.1 1176.2122 1175.3274 4490.3 9 2.908 65.0 1 K.IKGDESATVVR.S YLR357W 1 1 1.2% 889 102300 8.9 U RSC2 SGDID:S000004349, Chr XII from 841330-843999, Verified ORF, "One of 15 subunits of the 'Remodel the Structure of Chromatin' (RSC) complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook" * Jamie_Mitco_SPB_24.08577.08577.2 2.0207 0.0476 94.8 1368.1322 1367.5536 6607.3 411 3.096 50.0 1 K.LEGPLFVCEFR.Y YNL233W 1 1 1.2% 892 100590 5.1 U BNI4 SGDID:S000005177, Chr XIV from 211923-214601, Verified ORF, "Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p" * Jamie_Mitco_SPB_1.00026.00026.3 1.9527 0.1557 93.0 1328.0643 1326.4954 4807.8 27 3.767 42.5 1 K.TEVERDVPKPR.E Reverse_YGL234W 1 1 1.2% 802 86068 5.3 U ADE5,7 SGDID:S000003203, Chr VII from 56482-58890, Verified ORF, "Bifunctional enzyme of the 'de novo' purine nucleotide biosynthetic pathway, contains aminoimidazole ribotide synthetase and glycinamide ribotide synthetase activities" * Jamie_Mitco_SPB_9.03237.03237.2 1.4934 0.0468 85.1 1184.8121 1186.2181 4475.8 4 3.007 66.7 1 K.DTDLT*IKDGK.A YCL051W 1 1 1.2% 583 64792 8.1 U LRE1 SGDID:S000000556, Chr III from 35865-37616, Verified ORF, "Protein involved in control of cell wall structure and stress response; inhibits Cbk1p protein kinase activity; overproduction confers resistance to cell-wall degrading enzymes" * Jamie_Mitco_SPB_7.02314.02314.1 1.1138 0.1366 97.3 891.6 892.04877 4910.4 14 3.604 50.0 1 K.TRPRSFK.S YLR454W 1 1 1.1% 2628 303478 8.0 U YLR454W SGDID:S000004446, Chr XII from 1043995-1051881, Uncharacterized ORF, "The authentic, non-tagged protein was localized to the mitochondria" * Jamie_Mitco_SPB_55.19653.19653.3 3.2648 0.0161 86.6 3392.2744 3393.7349 7585.8 161 2.484 19.4 1 K.WGQLKELLEINFS*S*EYISQIIALPNGLR.T Reverse_YJL005W 1 1 1.1% 2026 227832 7.3 U CYR1 SGDID:S000003542, Chr X from 425072-431152, Verified ORF, "Adenylate cyclase, required for cAMP production and cAMP-dependent protein kinase signaling; involved in cell cycle control and glucose and nitrogen repression of sporulation" * Jamie_Mitco_SPB_30.10803.10803.2 1.96 0.0761 94.3 2460.6921 2459.5627 7348.1 123 3.137 26.2 1 K.NFESYFDSS*MAIQGGDAVGQVR.A YFL033C 1 1 1.1% 1770 196530 6.5 U RIM15 SGDID:S000001861, Chr VI from 74425-69113, reverse complement, Verified ORF, "Glucose-repressible protein kinase involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; originally identified as a regulator of IME2" * Jamie_Mitco_SPB_76.27022.27022.2 1.565 0.0099 81.0 2162.7122 2160.1729 7460.2 136 2.581 27.8 1 R.T*NSSDLSAEESDRLQAISR.V Reverse_YNL172W 1 1 1.1% 1748 196143 6.0 U APC1 SGDID:S000005116, Chr XIV from 310638-315884, Verified ORF, "Largest subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition" * Jamie_Mitco_SPB_23.07933.07933.2 2.1269 0.2802 91.7 2087.872 2088.3494 8157.6 19 4.982 33.3 1 A.RNTPAINNGMRDLTASLSR.R YNR070W 2 2 1.1% 1333 149750 8.0 U YNR070W SGDID:S000005353, Chr XIV from 765375-769376, Uncharacterized ORF, "Putative transporter of the ATP-binding cassette (ABC) family, implicated in pleiotropic drug resistance" * Jamie_Mitco_SPB_9.03234.03234.1 1.1506 0.0215 85.1 967.9 967.1456 3070.1 12 3.146 50.0 1 K.EMMQHYK.P Jamie_Mitco_SPB_8.02541.02541.1 1.0758 0.1083 95.5 870.52 869.86676 2868.8 26 3.407 57.1 1 S.GVS*GGERK.R Reverse_YAL001C 1 1 1.1% 1160 132108 9.4 U TFC3 SGDID:S000000001, Chr I from 151168-151099,151008-147596, reverse complement, Verified ORF, "Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding" * Jamie_Mitco_SPB_57.20181.20181.2 1.9631 0.1223 98.1 1573.6522 1575.0087 5167.1 215 3.335 41.7 1 K.ESKIALVLMKKWK.D YJL050W 1 1 1.1% 1073 122055 6.3 U MTR4 SGDID:S000003586, Chr X from 342438-345659, Verified ORF, "Dead-box family ATP dependent helicase required for mRNA export from the nucleus; co-factor of the exosome complex, required for 3' end formation of 5.8S rRNA" * Jamie_Mitco_SPB_12.04201.04201.1 1.0125 0.0509 86.4 1388.33 1387.5327 6307.5 36 3.43 27.3 1 K.GDAKGDIY*KIVK.M YJL084C 1 1 1.1% 1046 117216 6.9 U YJL084C SGDID:S000003620, Chr X from 277918-274778, reverse complement, Verified ORF, "Cytoplasmic protein of unknown function that interacts with Pcl7p, phosphorylated in vitro; potential Cdc28p substrate" * Jamie_Mitco_SPB_30.10486.10486.2 1.7831 0.0564 90.8 1591.5922 1591.8259 6112.6 236 3.567 36.4 1 R.EKKVHRMEPT*KK.F Reverse_YBR114W 1 1 1.1% 790 91430 8.3 U RAD16 SGDID:S000000318, Chr II from 467242-469614, Verified ORF, "Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad7p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4); member of the SWI/SNF family" * Jamie_Mitco_SPB_6.01962.01962.2 1.7765 0.0966 96.4 1048.4521 1049.212 5655.7 24 3.44 68.8 1 R.EYPTVKPSK.K Reverse_YPL207W 1 1 1.1% 810 89805 8.2 U YPL207W SGDID:S000006128, Chr XVI from 159908-162340, Uncharacterized ORF, "Hypothetical protein" * Jamie_Mitco_SPB_7.02232.02232.2 1.4366 0.0502 84.8 896.47217 898.05005 4606.6 45 3.497 56.2 1 R.EAIVGPVGR.M YIL092W 1 1 1.1% 633 71006 9.0 U YIL092W SGDID:S000001354, Chr IX from 189063-190964, Uncharacterized ORF, "Hypothetical protein" * Jamie_Mitco_SPB_11.03892.03892.2 1.854 0.0722 97.2 840.4322 840.96075 3406.8 11 3.241 75.0 1 K.RASNHKK.Y YBL005W-B 1 1 1.0% 1755 198968 8.2 U YBL005W-B SGDID:S000002147, Chr II from 221333-222637,222639-226601, transposable_element_gene, "TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition" YNL284C-B 1 1 1.0% 1755 198997 8.5 U YNL284C-B SGDID:S000007387, Chr XIV from 102232-100928,100926-96964, reverse complement, transposable_element_gene, "TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition" YMR045C 1 1 1.0% 1755 198898 8.3 U YMR045C SGDID:S000004648, Chr XIII from 362626-361322,361320-357358, reverse complement, transposable_element_gene, "TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition" YJR029W 1 1 1.0% 1755 198616 8.3 U YJR029W SGDID:S000003790, Chr X from 478258-479558,479560-483526, transposable_element_gene, "TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition" YGR027W-B 1 1 1.0% 1755 198656 8.2 U YGR027W-B SGDID:S000007406, Chr VII from 536061-537365,537367-541329, transposable_element_gene, "TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition" Jamie_Mitco_SPB_19.06557.06557.2 2.2473 0.2098 87.8 1996.8121 1998.2556 4876.9 251 4.394 34.4 1 K.LKMQYDTKIINLGESDE.E Reverse_YGL206C 1 1 1.0% 1653 187233 5.2 U CHC1 SGDID:S000003174, Chr VII from 107508-102547, reverse complement, Verified ORF, "Clathrin heavy chain, subunit of the major coat protein involved in intracellular protein transport and endocytosis; two heavy chains form the clathrin triskelion structural component; the light chain (CLC1) is thought to regulate function" * Jamie_Mitco_SPB_12.04273.04273.2 1.4341 0.203 96.8 2126.152 2125.2993 5303.4 299 4.521 30.0 1 K.VY*FNIAKYYIELNS*VK.V YLL021W 1 1 1.0% 1466 163142 5.2 U SPA2 SGDID:S000003944, Chr XII from 100946-105346, Verified ORF, "Component of the polarisome, which functions in actin cytoskeletal organization during polarized growth; acts as a scaffold for Mkk1p and Mpk1p cell wall integrity signaling components; potential Cdc28p substrate" * Jamie_Mitco_SPB_31.10827.10827.2 2.1813 0.2092 88.8 1567.9722 1565.5046 8049.8 25 4.206 46.2 1 S.ASNSASS*NIS*EIPK.L YIL126W 1 1 1.0% 1359 156742 6.7 U STH1 SGDID:S000001388, Chr IX from 117992-122071, Verified ORF, "ATPase component of the ATP-dependent RSC chromatin remodeling complex required for kinetochore function in chromosome segregation; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p" * Jamie_Mitco_SPB_56.19914.19914.2 1.7258 0.0755 90.8 1623.9922 1625.6006 6385.6 3 3.336 50.0 1 K.SS*SVEIINGSES*KK.K Reverse_YOR296W 1 1 1.0% 1289 147426 8.6 U YOR296W SGDID:S000005822, Chr XV from 870199-874068, Uncharacterized ORF, "Hypothetical protein" * Jamie_Mitco_SPB_29.10261.10261.2 1.4898 0.1899 97.3 1568.1921 1567.8264 4259.6 108 3.895 37.5 1 K.KKVTT*EGKKLNIK.Y YKL209C 1 1 1.0% 1290 144765 8.0 U STE6 SGDID:S000001692, Chr XI from 46296-42424, reverse complement, Verified ORF, "ATP-binding cassette (ABC) transporter required for the export of a-factor, catalyzes ATP hydrolysis coupled to a-factor transport, contains 12 transmembrane domains and two ATP binding domains, expressed only in MATa cells" * Jamie_Mitco_SPB_120.43174.43174.2 1.8537 0.2529 88.0 1342.1122 1341.4178 8792.0 377 3.888 45.8 1 G.QTLGIIGESGT*GK.S YPL217C 1 1 1.0% 1183 135571 6.8 U BMS1 SGDID:S000006138, Chr XVI from 143170-139619, reverse complement, Verified ORF, "Essential conserved nucleolar GTP-binding protein required for synthesis of 40S ribosomal subunits and for processing of the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, has similarity to Tsr1p" * Jamie_Mitco_SPB_21.07331.07331.2 2.2159 0.2824 100.0 1219.8922 1219.383 2579.8 1 4.499 68.2 1 R.FLGAGILGNDNK.T YPL019C 1 1 1.0% 835 96553 7.4 U VTC3 SGDID:S000005940, Chr XVI from 517016-514509, reverse complement, Verified ORF, "Vacuolar membrane protein involved in vacuolar polyphosphate accumulation; functions as a regulator of vacuolar H+-ATPase activity and vacuolar transporter chaperones; involved in non-autophagic vacuolar fusion" * Jamie_Mitco_SPB_8.02806.02806.2 1.7418 0.0581 93.0 1043.5122 1043.2535 5123.6 54 3.23 71.4 1 K.FINNFIFK.N Reverse_YNL225C 1 1 1.0% 581 67400 6.0 U CNM67 SGDID:S000005169, Chr XIV from 224470-222725, reverse complement, Verified ORF, "Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration" YJL105W 1 1 1.1% 560 63856 8.7 U SET4 SGDID:S000003641, Chr X from 224972-226654, Verified ORF, "Protein of unknown function, contains a SET domain" Jamie_Mitco_SPB_21.07218.07218.2 1.9392 0.0555 97.9 801.8122 800.7605 2505.0 6 3.344 90.0 1 H.KSS*NER.P YCR093W 1 1 0.9% 2108 240277 6.4 U CDC39 SGDID:S000000689, Chr III from 280113-286439, Verified ORF, "Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor" * Jamie_Mitco_SPB_63.22469.22469.2 1.8561 0.1031 96.2 2416.2922 2415.6343 7049.2 98 3.507 26.3 1 R.MDFIKTLEHLASS*EDENVKK.F Reverse_YLR422W 1 1 0.9% 1932 221561 7.8 U YLR422W SGDID:S000004414, Chr XII from 965893-971691, Uncharacterized ORF, "Hypothetical protein" * Jamie_Mitco_SPB_44.15576.15576.2 1.6099 0.0284 83.6 2118.632 2117.2402 7872.5 32 2.822 29.4 1 R.S*ECEKSILPKFDEASPAK.F YNL172W 1 1 0.9% 1748 196143 6.0 U APC1 SGDID:S000005116, Chr XIV from 310638-315884, Verified ORF, "Largest subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition" * Jamie_Mitco_SPB_39.14004.14004.2 1.6663 0.0491 88.0 1841.8322 1842.1924 7250.0 45 3.263 35.7 1 R.RLSSILSKLKFLS*LR.F Reverse_YDR093W 1 1 0.9% 1612 182618 6.2 U DNF2 SGDID:S000002500, Chr IV from 631277-636115, Verified ORF, "Non-essential P-type ATPase that is a potential aminophospholipid translocase, localizes to the plasma membrane and late exocytic or early endocytic membranes, likely involved in protein transport; potential Cdc28p substrate" * Jamie_Mitco_SPB_31.10866.10866.2 2.0711 0.2254 87.4 1627.4722 1626.8566 7209.6 12 4.688 39.3 1 K.CILLAKPEDKPTSGP.I Reverse_YHL035C 1 1 0.9% 1592 180925 8.5 U YHL035C SGDID:S000001027, Chr VIII from 32754-27976, reverse complement, Uncharacterized ORF, "Protein of unknown function, member of the ATP-binding cassette (ABC) family, potential Cdc28p substrate" * Jamie_Mitco_SPB_81.29065.29065.2 1.8733 0.242 81.2 1811.0922 1808.92 5701.9 4 4.352 42.9 1 Y.YFANHKNKSS*DTMGK.L YPR184W 1 1 0.9% 1536 174971 5.8 U GDB1 SGDID:S000006388, Chr XVI from 902040-906650, Verified ORF, "Glycogen debranching enzyme containing glucanotranferase and alpha-1,6-amyloglucosidase activities, required for glycogen degradation" * Jamie_Mitco_SPB_60.21441.21441.2 1.8142 0.0515 90.2 1724.0922 1726.9725 6610.9 84 3.267 38.5 1 K.LWEYYAVNVQTALR.D Reverse_YAL029C 1 1 0.9% 1471 169343 7.6 U MYO4 SGDID:S000000027, Chr I from 92271-87856, reverse complement, Verified ORF, "One of two type V myosins; required for mother-specific HO expression, for the bud tip localization of ASH1 and IST2 mRNA; facilitates growth and orientation of ER tubules along with She3p" * Jamie_Mitco_SPB_20.07143.07143.2 2.8703 0.0594 99.4 1560.0922 1560.8772 6518.7 3 3.107 70.8 1 K.FENLNSIKLQLIK.V Reverse_YOR330C 1 1 0.9% 1254 143502 8.8 U MIP1 SGDID:S000005857, Chr XV from 943380-939616, reverse complement, Verified ORF, "Catalytic subunit of the mitochondrial DNA polymerase" * Jamie_Mitco_SPB_44.15826.15826.2 1.6007 0.1786 97.9 1302.7722 1303.3708 4832.3 7 3.595 55.0 1 K.DTSAFFDRDTK.D Reverse_YKL129C 1 1 0.9% 1271 142507 9.4 U MYO3 SGDID:S000001612, Chr XI from 200163-196348, reverse complement, Verified ORF, "One of two type I myosins; localizes to actin cortical patches; deletion of MYO3 has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization" * Jamie_Mitco_SPB_20.06991.06991.2 1.4789 0.1259 91.6 1394.4122 1392.6847 4774.2 387 3.432 36.4 1 K.KKNADFTAKKIK.A YIL147C 1 1 0.9% 1220 134435 7.5 U SLN1 SGDID:S000001409, Chr IX from 73453-69791, reverse complement, Verified ORF, "Histidine kinase osmosensor that regulates a MAP kinase cascade; transmembrane protein with an intracellular kinase domain that signals to Ypd1p and Ssk1p, thereby forming a phosphorelay system similar to bacterial two-component regulators" * Jamie_Mitco_SPB_45.16092.16092.2 1.5824 0.0094 82.5 1341.1522 1342.5459 3333.9 123 2.976 45.0 1 R.QLANMMHGT*MK.L Reverse_YBL101C 1 1 0.9% 1117 123609 6.9 U ECM21 SGDID:S000000197, Chr II from 28299-24946, reverse complement, Verified ORF, "Non-essential protein of unknown function; promoter contains several Gcn4p binding elements" * Jamie_Mitco_SPB_7.02501.02501.2 2.1219 0.0269 95.5 1103.4521 1103.1771 5896.7 3 3.029 66.7 1 K.SNETTANPIR.V Reverse_YDR122W 1 1 0.9% 1064 120071 8.7 U KIN1 SGDID:S000002529, Chr IV from 694695-697889, Verified ORF, "Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin2p" * Jamie_Mitco_SPB_18.06226.06226.2 1.7854 0.089 96.0 1247.5922 1246.4526 6069.2 89 3.287 55.6 1 R.ITRKDRSIEK.E YDR028C 1 1 0.9% 1014 112616 5.1 U REG1 SGDID:S000002435, Chr IV from 500875-497831, reverse complement, Verified ORF, "Regulatory subunit of type 1 protein phosphatase Glc7p, involved in negative regulation of glucose-repressible genes" * Jamie_Mitco_SPB_13.04409.04409.2 1.4374 0.0484 84.4 951.8122 951.0214 4984.5 72 3.352 62.5 1 K.ESNSSVSLK.S YNL123W 1 1 0.9% 997 110881 6.0 U YNL123W SGDID:S000005067, Chr XIV from 394687-397680, Uncharacterized ORF, "shows protein sequence similarity to the mammalian Omi/HtrA2 family of serine proteases" * Jamie_Mitco_SPB_9.03168.03168.2 1.7473 0.0067 87.8 1087.9521 1087.1313 5765.1 181 2.706 68.8 1 R.EGPGY*DKIK.E Reverse_YOR219C 1 1 0.9% 931 107200 6.0 U STE13 SGDID:S000005745, Chr XV from 755009-752214, reverse complement, Verified ORF, "Dipeptidyl aminopeptidase, Golgi integral membrane protein that cleaves on the carboxyl side of repeating -X-Ala- sequences, required for maturation of alpha factor, transcription is induced by a-factor" * Jamie_Mitco_SPB_12.04318.04318.2 1.3831 0.0577 84.5 881.5122 882.05066 5533.9 331 3.563 64.3 1 K.KPNLNAPK.I YJR126C 1 1 0.9% 811 92018 7.2 U VPS70 SGDID:S000003887, Chr X from 658604-656169, reverse complement, Verified ORF, "Protein of unknown function involved in vacuolar protein sorting" * Jamie_Mitco_SPB_11.03951.03951.1 1.0065 0.0631 88.6 1034.69 1034.9255 2552.3 12 2.693 41.7 1 K.FT*NS*DYK.L YNL186W 1 2 0.9% 792 88517 5.5 U UBP10 SGDID:S000005130, Chr XIV from 289500-291878, Verified ORF, "Ubiquitin-specific protease that deubiquitinates ubiquitin-protein moieties; may regulate silencing by acting on Sir4p; involved in posttranscriptionally regulating Gap1p and possibly other transporters; primarily located in the nucleus" * Jamie_Mitco_SPB_18.06136.06136.2 1.6947 0.0672 93.4 833.1922 831.9908 3552.9 253 3.451 66.7 2 R.KASLKER.D YNL161W 1 1 0.9% 756 86946 7.9 U CBK1 SGDID:S000005105, Chr XIV from 332599-334869, Verified ORF, "Serine/threonine protein kinase that regulates cell morphogenesis pathways; involved in cell wall biosynthesis, apical growth, proper mating projection morphology, bipolar bud site selection in diploid cells, and cell separation" * Jamie_Mitco_SPB_14.04978.04978.2 1.372 0.0285 82.1 844.0122 843.01404 3470.7 43 2.898 66.7 1 R.RPDLLTK.G YLL040C 1 1 0.8% 3144 357850 5.6 U VPS13 SGDID:S000003963, Chr XII from 63644-54210, reverse complement, Verified ORF, "Protein of unknown function; heterooligomeric or homooligomeric complex; peripherally associated with membranes; homologous to human COH1; involved in sporulation, vacuolar protein sorting and protein-Golgi retention" * Jamie_Mitco_SPB_50.17940.17940.2 1.4339 0.0557 83.0 2947.0322 2946.2537 7095.1 211 2.923 20.8 1 R.S*IREKLDIDKSKNILGVCVSGQNYK.T YNL262W 2 2 0.8% 2222 255669 7.0 U POL2 SGDID:S000005206, Chr XIV from 148213-154881, Verified ORF, "Catalytic subunit of DNA polymerase epsilon, one of the major chromosomal DNA replication polymerases characterized by processivity and proofreading exonuclease activity; also involved in DNA synthesis during DNA repair" * Jamie_Mitco_SPB_75.26929.26929.2 1.6951 0.0444 87.9 1860.0322 1857.3401 7869.3 137 3.156 30.0 1 K.IKIARTTKKKAVSKRK.R * Jamie_Mitco_SPB_70.24928.24928.2 1.4761 0.0053 80.6 1510.6721 1511.7683 4334.2 148 2.443 40.9 1 R.T*TKKKAVSKRKR.N Reverse_YMR207C 1 1 0.8% 2123 241784 8.0 U HFA1 SGDID:S000004820, Chr XIII from 683563-677192, reverse complement, Verified ORF, "Mitochondrial acetyl-coenzyme A carboxylase, catalyzes the production of malonyl-CoA in mitochondrial fatty acid biosynthesis" * Jamie_Mitco_SPB_20.07060.07060.2 1.6924 0.0588 88.7 1714.1522 1713.8424 6500.9 117 3.132 36.7 1 K.ASQAVITSSIKDGLS*R.M YDL058W 1 1 0.8% 1790 206450 4.9 U USO1 SGDID:S000002216, Chr IV from 345665-351037, Verified ORF, "Essential protein involved in intracellular protein transport, coiled-coil protein necessary for transport from ER to Golgi; required for assembly of the ER-to-Golgi SNARE complex" * Jamie_Mitco_SPB_28.09750.09750.2 2.1272 0.0205 91.7 1615.4922 1614.8798 12002.8 78 2.738 50.0 1 K.SEIDALNLQIKELK.K YDR283C 1 1 0.8% 1659 190193 6.4 U GCN2 SGDID:S000002691, Chr IV from 1030043-1025064, reverse complement, Verified ORF, "Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex" * Jamie_Mitco_SPB_85.30528.30528.2 1.8112 0.1381 97.4 1540.7522 1541.7501 5079.0 95 3.384 45.8 1 K.ARNALDSRYYAIK.K YGR098C 1 1 0.8% 1630 187445 8.2 U ESP1 SGDID:S000003330, Chr VII from 687462-682570, reverse complement, Verified ORF, "Separase with cysteine protease activity (related to caspases) that promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibited by Pds1p" * Jamie_Mitco_SPB_112.40213.40213.1 1.2256 0.2683 80.0 1587.68 1587.6409 6355.1 3 4.248 33.3 1 L.TLTILSNIT*S*IHN.A Reverse_YNL088W 1 1 0.8% 1428 164214 7.0 U TOP2 SGDID:S000005032, Chr XIV from 457706-461992, Verified ORF, "Essential type II topoisomerase, relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis" * Jamie_Mitco_SPB_10.03425.03425.2 1.7672 0.0953 95.0 1256.5922 1256.4875 5430.3 122 3.016 55.0 1 K.FTVKTYSPGKK.Y Reverse_YDR464W 1 1 0.8% 1435 161596 8.9 U SPP41 SGDID:S000002872, Chr IV from 1388862-1393169, Verified ORF, "Protein involved in negative regulation of expression of spliceosome components PRP4 and PRP3" * Jamie_Mitco_SPB_16.05502.05502.2 2.1553 0.0125 93.0 1405.8922 1406.4465 5432.1 3 3.128 60.0 1 K.KELSVET*KS*PK.K Reverse_YCR073C 1 1 0.8% 1331 152717 7.7 U SSK22 SGDID:S000000669, Chr III from 246579-242584, reverse complement, Verified ORF, "MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; functionally redundant with, and homologous to, Ssk2p; interacts with and is activated by Ssk1p; phosphorylates Pbs2p" * Jamie_Mitco_SPB_18.06131.06131.2 2.022 0.0662 96.7 1339.5521 1340.3464 5748.4 1 2.897 72.2 1 K.FNQKS*S*DILK.L Reverse_YLR425W 1 1 0.8% 1307 149112 6.9 U TUS1 SGDID:S000004417, Chr XII from 982890-986813, Verified ORF, "Guanine nucleotide exchange factor (GEF) that functions to modulate Rhop1 activity as part of the cell integrity signaling pathway; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate" * Jamie_Mitco_SPB_8.02710.02710.2 1.3785 0.024 80.7 1188.9321 1188.3666 5734.1 257 3.087 55.6 1 K.KYVT*GSLVLK.R YDL031W 1 1 0.8% 995 113158 9.3 U DBP10 SGDID:S000002189, Chr IV from 394214-397201, Verified ORF, "Putative ATP-dependent RNA helicase of the DEAD-box protein family, constituent of 66S pre-ribosomal particles; essential protein involved in ribosome biogenesis" * Jamie_Mitco_SPB_12.04053.04053.2 2.4286 0.1017 99.8 987.4522 987.11615 4131.6 16 3.336 71.4 1 K.KT*AMKAEK.L YBR038W 1 1 0.8% 963 109882 8.6 U CHS2 SGDID:S000000242, Chr II from 311897-314788, Verified ORF, "Chitin synthase II, requires activation from zymogenic form in order to catalyze the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for the synthesis of chitin in the primary septum during cytokinesis" * Jamie_Mitco_SPB_113.40356.40356.1 1.6272 0.2212 100.0 861.38 862.83136 3131.2 3 4.17 57.1 1 D.SAHNS*PVA.P Reverse_YKR054C 3 3 0.7% 4092 471351 6.3 U DYN1 SGDID:S000001762, Chr XI from 547567-535289, reverse complement, Verified ORF, "Cytoplasmic heavy chain dynein, microtubule motor protein, required for anaphase spindle elongation; involved in spindle assembly, chromosome movement, and spindle orientation during cell division, targeted to microtubule tips by Pac1p" * Jamie_Mitco_SPB_4.01171.01171.2 1.6024 0.0828 91.4 971.9322 971.23145 6965.6 3 3.139 68.8 1 K.ATVLKVLAR.L * Jamie_Mitco_SPB_7.02226.02226.2 1.4674 0.0097 80.7 1124.8522 1125.2694 5953.9 186 2.833 55.6 1 R.LGDIFYGPSR.P * Jamie_Mitco_SPB_30.10749.10749.2 1.3772 0.0348 82.4 893.0522 890.0708 2687.2 305 3.052 50.0 1 R.TLITKGTR.S YKR054C 2 2 0.7% 4092 471351 6.3 U DYN1 SGDID:S000001762, Chr XI from 547567-535289, reverse complement, Verified ORF, "Cytoplasmic heavy chain dynein, microtubule motor protein, required for anaphase spindle elongation; involved in spindle assembly, chromosome movement, and spindle orientation during cell division, targeted to microtubule tips by Pac1p" * Jamie_Mitco_SPB_32.11194.11194.2 1.5778 0.0327 83.4 1687.6522 1686.1558 5844.9 246 2.847 39.3 1 K.LLGSVMRALTKLKVR.F * Jamie_Mitco_SPB_15.05304.05304.2 1.8361 0.0174 87.9 1637.7122 1637.917 5953.5 121 3.253 38.5 1 R.SISLVKSLTFEKER.W YJR066W 1 1 0.7% 2470 281138 7.2 U TOR1 SGDID:S000003827, Chr X from 559330-566742, Verified ORF, "PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis" * Jamie_Mitco_SPB_38.13383.13383.3 2.8662 0.0512 90.6 1926.0543 1923.3464 6893.0 65 3.8 34.4 1 K.DVAKPYIEPLLNVLLPK.F Reverse_YJL130C 1 1 0.7% 2214 245124 5.9 U URA2 SGDID:S000003666, Chr X from 172285-165641, reverse complement, Verified ORF, "Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes the first two enzymatic steps in the de novo biosynthesis of pyrimidines; both activities are subject to feedback inhibition by UTP" * Jamie_Mitco_SPB_93.33127.33127.2 1.9044 0.248 85.4 1523.1921 1521.54 4150.9 10 4.71 42.9 1 K.ENNAFGSGLGGLAY*A.A Reverse_YPL231W 1 1 0.7% 1887 206945 5.4 U FAS2 SGDID:S000006152, Chr XVI from 108652-114315, Verified ORF, "Alpha subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains beta-ketoacyl reductase and beta-ketoacyl synthase activities" * Jamie_Mitco_SPB_32.11351.11351.2 1.6013 0.0048 81.8 1598.5122 1597.7618 9285.1 349 3.05 34.6 1 K.AALESPDPTYYIEK.A Reverse_YJL129C 1 1 0.7% 1235 141073 8.5 U TRK1 SGDID:S000003665, Chr X from 177224-173517, reverse complement, Verified ORF, "Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter" * Jamie_Mitco_SPB_115.41268.41268.1 1.3466 0.0947 96.1 902.02 903.02625 3209.7 6 3.444 56.2 1 K.NGNIGTAKK.L YPL110C 1 1 0.7% 1223 138014 6.8 U GDE1 SGDID:S000006031, Chr XVI from 344738-341067, reverse complement, Verified ORF, "Glycerophosphocholine (GroPCho) phosphodiesterase; hydrolyzes GroPCho to choline and glycerolphosphate after its uptake by Git1p permease, for use as a phosphate source and as a precursor for phosphocholine synthesis" * Jamie_Mitco_SPB_8.02626.02626.2 1.9879 0.0239 93.1 944.1322 946.0513 3730.6 148 3.241 62.5 1 R.ASSLQNGIR.F YDR096W 1 1 0.7% 894 99481 7.5 U GIS1 SGDID:S000002503, Chr IV from 637134-639818, Verified ORF, "Transcriptional factor, involved in the expression of genes during nutrient limitation; also involved in the negative regulation of DPP1 and PHR1" * Jamie_Mitco_SPB_14.05015.05015.2 1.4378 0.019 83.3 821.33215 821.6897 4020.8 198 2.945 70.0 1 K.EPS*S*NK.E Reverse_YPL008W 1 1 0.7% 861 98806 7.9 U CHL1 SGDID:S000005929, Chr XVI from 539382-541967, Verified ORF, "Conserved nuclear protein required to establish sister-chromatid pairing during S-phase, probable DNA helicase with similarity to human BACH1, which associates with tumor suppressor BRCA1; associates with acetyltransferase Ctf7p" * Jamie_Mitco_SPB_77.27376.27376.1 1.1048 0.0232 83.9 732.25 732.8179 2783.3 88 2.523 60.0 1 -.RSNLSR.M YOR322C 1 1 0.7% 818 89826 8.6 U LDB19 SGDID:S000005849, Chr XV from 921057-918601, reverse complement, Verified ORF, "Protein of unknown function; null mutant shows a reduced affinity for the alcian blue dye suggesting a decreased net negative charge of the cell surface" * Jamie_Mitco_SPB_75.26980.26980.1 1.2871 0.0275 92.5 717.47 718.82837 3246.2 15 3.381 70.0 1 R.LTVTER.S Reverse_YGL195W 1 1 0.6% 2672 296696 5.3 U GCN1 SGDID:S000003163, Chr VII from 131531-139549, Verified ORF, "Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn20p; proposed to stimulate Gcn2p activation by an uncharged tRNA" * Jamie_Mitco_SPB_7.02278.02278.3 2.4791 0.2895 83.3 1795.7943 1798.8987 5435.8 8 5.176 35.9 1 R.NEIS*LETSLAATLIADG.I Reverse_YLR305C 1 1 0.6% 1900 214606 7.5 U STT4 SGDID:S000004296, Chr XII from 743865-738163, reverse complement, Verified ORF, "Phosphatidylinositol-4-kinase that functions in the Pkc1p protein kinase pathway; required for normal vacuole morphology, cell wall integrity, and actin cytoskeleton organization" * Jamie_Mitco_SPB_35.12471.12471.3 2.1384 0.0862 87.8 1480.1943 1483.5381 2519.1 302 3.306 40.9 1 R.LSS*S*AKLGRTFR.M Reverse_YFL033C 1 1 0.6% 1770 196530 6.5 U RIM15 SGDID:S000001861, Chr VI from 74425-69113, reverse complement, Verified ORF, "Glucose-repressible protein kinase involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; originally identified as a regulator of IME2" * Jamie_Mitco_SPB_21.07309.07309.2 1.7924 0.1351 97.8 1242.4521 1240.1608 5355.7 47 3.578 50.0 1 K.MAQSGGAT*NS*R.N YDR507C 1 1 0.6% 1142 129858 9.2 U GIN4 SGDID:S000002915, Chr IV from 1465776-1462348, reverse complement, Verified ORF, "Protein kinase involved in bud growth and assembly of the septin ring, proposed to have kinase-dependent and kinase-independent activities; undergoes autophosphorylation; similar to Kcc4p and Hsl1p" * Jamie_Mitco_SPB_11.03905.03905.2 2.0394 0.0027 94.6 1036.2922 1036.0066 3721.7 64 2.975 75.0 1 K.KNKRS*S*R.S Reverse_YHR165C 1 1 0.5% 2413 279502 7.3 U PRP8 SGDID:S000001208, Chr VIII from 436950-429709, reverse complement, Verified ORF, "Component of the U4/U6-U5 snRNP complex, involved in the second catalytic step of splicing" * Jamie_Mitco_SPB_28.09766.09766.4 2.6057 0.1592 91.5 1722.6166 1721.9761 2965.7 463 3.309 33.3 1 K.AKKT*MKKARKRS*K.R YPL085W 1 1 0.5% 2195 241694 5.4 U SEC16 SGDID:S000006006, Chr XVI from 387064-393651, Verified ORF, "COPII vesicle coat protein required for ER transport vesicle budding and autophagosome formation; Sec16p is bound to the periphery of ER membranes and may act to stabilize initial COPII complexes; interacts with Sec23p, Sec24p and Sec31p" * Jamie_Mitco_SPB_45.15940.15940.2 1.7991 0.0817 94.1 1337.6122 1335.5461 4343.9 67 3.254 55.0 1 K.PVEEKKASRYK.P YGR032W 1 1 0.5% 1895 216988 7.0 U GSC2 SGDID:S000003264, Chr VII from 548268-553955, Verified ORF, "Catalytic subunit of 1,3-beta-glucan synthase, has similarity to an alternate catalytic subunit, Fks1p (Gsc1p); Rho1p encodes the regulatory subunit; involved in cell wall synthesis and maintenance" * Jamie_Mitco_SPB_114.41009.41009.1 1.9664 0.2025 100.0 994.14 995.12335 4217.6 145 5.114 50.0 1 V.ASAHVPKDLG.S Reverse_YIR006C 1 1 0.5% 1480 160266 5.3 U PAN1 SGDID:S000001445, Chr IX from 369905-365463, reverse complement, Verified ORF, "Part of actin cytoskeleton-regulatory complex Pan1p-Sla1p-End3p, associates with actin patches on the cell cortex; promotes protein-protein interactions essential for endocytosis; previously thought to be a subunit of poly(A) ribonuclease" * Jamie_Mitco_SPB_6.01869.01869.1 1.35 6.0E-4 86.1 846.38 847.00244 4970.9 63 3.16 66.7 1 R.KEKDAKK.K YLR106C 1 1 0.4% 4910 559316 5.0 U MDN1 SGDID:S000004096, Chr XII from 363739-349007, reverse complement, Verified ORF, "Huge dynein-related AAA-type ATPase (midasin), forms extended pre-60S particle with the Rix1 complex (Rix1p-Ipi1p-Ipi3p), may mediate ATP-dependent remodeling of 60S subunits and subsequent export from nucleoplasm to cytoplasm" * Jamie_Mitco_SPB_105.37522.37522.3 2.4823 0.1247 92.9 2389.4343 2386.6387 3862.6 427 3.295 27.8 1 R.LLQFSESYNS*IKDLMDVFR.L YOL081W 1 1 0.4% 3079 351669 7.1 U IRA2 SGDID:S000005441, Chr XV from 171069-180308, Verified ORF, "GTPase-activating protein that negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, has similarity to Ira1p and human neurofibromin" * Jamie_Mitco_SPB_15.05344.05344.2 1.8059 0.0176 87.9 1514.0922 1512.4858 6733.9 1 2.699 58.3 1 K.HDDGDIDGDDFYK.T Reverse_YJR066W 1 1 0.4% 2470 281138 7.2 U TOR1 SGDID:S000003827, Chr X from 559330-566742, Verified ORF, "PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis" * Jamie_Mitco_SPB_41.14407.14407.2 1.503 0.024 82.8 1352.4321 1352.4435 6463.6 87 3.102 50.0 1 K.Y*DSTLERFIK.E YHR023W 1 1 0.4% 1928 223634 6.4 U MYO1 SGDID:S000001065, Chr VIII from 151659-157445, Verified ORF, "Type II myosin heavy chain, required for wild-type cytokinesis and cell separation; localizes to the actomyosin ring; binds to myosin light chains Mlc1p and Mlc2p through its IQ1 and IQ2 motifs respectively" * Jamie_Mitco_SPB_4.01411.01411.2 1.6374 0.0817 92.9 1004.53217 1004.08496 6535.0 27 3.189 71.4 1 K.KELAT*FSK.Q Reverse_YJR138W 1 1 0.4% 1584 181948 8.8 U IML1 SGDID:S000003899, Chr X from 684482-689236, Verified ORF, "Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the vacuolar membrane" * Jamie_Mitco_SPB_10.03289.03289.1 1.0897 0.0103 80.2 903.0 904.1026 2411.4 241 2.463 41.7 1 K.PGRWIFK.T Reverse_YLL040C 1 1 0.3% 3144 357850 5.6 U VPS13 SGDID:S000003963, Chr XII from 63644-54210, reverse complement, Verified ORF, "Protein of unknown function; heterooligomeric or homooligomeric complex; peripherally associated with membranes; homologous to human COH1; involved in sporulation, vacuolar protein sorting and protein-Golgi retention" * Jamie_Mitco_SPB_7.02254.02254.1 1.0334 0.0542 87.0 914.62 914.1356 4976.2 27 3.224 50.0 1 K.PFQIIPAK.I Reverse_YBR140C 1 1 0.3% 3092 350980 6.6 U IRA1 SGDID:S000000344, Chr II from 526622-517344, reverse complement, Verified ORF, "GTPase-activating protein that negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase" * Jamie_Mitco_SPB_64.22720.22720.2 1.599 0.0622 89.1 1361.7922 1361.5864 4537.7 118 3.352 50.0 1 R.FEKRTLTYFR.F Reverse_YLR087C 1 1 0.3% 2958 338258 5.9 U CSF1 SGDID:S000004077, Chr XII from 315732-306856, reverse complement, Verified ORF, "Protein required for fermentation at low temperature" * Jamie_Mitco_SPB_38.13434.13434.2 1.5564 0.033 85.3 1217.7922 1215.2627 3268.3 46 3.361 50.0 1 K.SILET*KSNSR.T Reverse_YER155C 1 1 0.3% 2167 245429 8.4 U BEM2 SGDID:S000000957, Chr V from 482843-476340, reverse complement, Verified ORF, "Rho GTPase activating protein (RhoGAP) involved in the control of cytoskeleton organization and cellular morphogenesis; required for bud emergence" * Jamie_Mitco_SPB_9.03203.03203.1 1.0846 0.0135 81.0 853.89 853.9939 3573.0 225 2.85 50.0 1 K.IHIDDIK.E YLR087C 1 1 0.2% 2958 338258 5.9 U CSF1 SGDID:S000004077, Chr XII from 315732-306856, reverse complement, Verified ORF, "Protein required for fermentation at low temperature" * Jamie_Mitco_SPB_22.07869.07869.2 1.3814 0.0512 84.4 832.7322 834.9077 3379.6 178 3.006 58.3 1 K.KLPPGS*R.R Proteins Peptide IDs Spectra Unfiltered 1702 86256 90927 Filtered 656 1848 2072 Forward matches 407 1517 1737 Decoy matches 249 331 335 Forward FP rate 61.18 21.82 19.29 Classification Nonredundant Proteins Redundant Proteins Unclassified 0 0