DTASelect v2.0.28 /data/1/catclw/Projects/Jamie/Jamie_June2008/Alpha_09102008/splitted /scratch/yates/SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta SEQUEST 3.0 in SQT format. --trypstat --cmax 4 --fp 0.1 -p 1 true Use criteria 0.0 Minimum peptide confidence 0.1 Peptide false positive rate 0.0 Minimum protein confidence 1.0 Protein false positive rate 1 Minimum charge state 16 Maximum charge state 0.0 Minimum ion proportion 1000 Maximum Sp rank -1.0 Minimum Sp score Include Modified peptide inclusion Any Tryptic status requirement false Multiple, ambiguous IDs allowed Ignore Peptide validation handling XCorr Purge duplicate peptides by protein false Include only loci with unique peptide true Remove subset proteins Ignore Locus validation handling 0 Minimum modified peptides per locus 1000 Minimum redundancy for low coverage loci 1 Minimum peptides per locus Locus Sequence Count Spectrum Count Sequence Coverage Length MolWt pI Validation Status Descriptive Name Unique FileName XCorr DeltCN Conf% M+H+ CalcM+H+ TotalIntensity SpR ZScore IonProportion Redundancy Sequence YIL002W-A 9 11 56.5% 69 7729 4.7 U YIL002W-A SGDID:S000028835, Chr IX from 350298-350507, Uncharacterized ORF, "Identified by expression profiling and mass spectrometry" * Jamie_alpha_091208_11.04393.04393.2 3.5373 0.1756 100.0 2063.0205 2064.3125 8254.1 1 6.046 52.9 1 K.GGEEKISEVELKLDEMEK.K * Jamie_alpha_091208_11.04344.04344.3 4.5712 0.1636 100.0 2064.0286 2064.3125 7572.0 1 4.506 45.6 1 K.GGEEKISEVELKLDEMEK.K * Jamie_alpha_091208_10.03839.03839.3 3.419 0.155 98.0 2192.112 2192.4866 8596.0 4 3.976 34.7 1 K.GGEEKISEVELKLDEMEKK.M * Jamie_alpha_091208_10.03945.03945.2 3.1965 0.2461 100.0 2193.8386 2192.4866 6008.2 1 4.5 44.4 1 K.GGEEKISEVELKLDEMEKK.M * Jamie_alpha_091208_7.03021.03021.2 3.0476 0.2609 100.0 1691.8717 1691.9777 6286.0 3 4.943 53.8 1 K.ISEVELKLDEMEKK.M * Jamie_alpha_091208_15.05684.05684.3 4.4999 0.2941 100.0 2466.2615 2468.7876 6425.9 9 5.178 32.5 2 K.KMDSLLVQLEDLHRDNNDLAK.S * Jamie_alpha_091208_15.05717.05717.2 3.7645 0.2206 100.0 2468.2676 2468.7876 6819.3 1 4.723 47.5 1 K.KMDSLLVQLEDLHRDNNDLAK.S * Jamie_alpha_091208_16.06320.06320.2 3.4788 0.2216 100.0 2340.1748 2340.6135 7118.1 1 4.758 44.7 1 K.MDSLLVQLEDLHRDNNDLAK.S * Jamie_alpha_091208_17.06334.06334.3 4.0614 0.1248 97.9 2345.0764 2340.6135 5702.3 1 4.063 42.1 2 K.MDSLLVQLEDLHRDNNDLAK.S YIL149C 73 80 50.5% 1679 195140 6.0 U MLP2 SGDID:S000001411, Chr IX from 68067-63028, reverse complement, Verified ORF, "Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved in the Tel1p pathway that controls telomere length" * Jamie_alpha_091208_22.08335.08335.4 4.5788 0.1967 100.0 3374.765 3375.8035 7053.8 4 4.094 23.5 1 K.IAKFERSEEEVTKLNVLVDEIKSQYYSR.I * Jamie_alpha_091208_22.08227.08227.5 5.764 0.2433 100.0 3375.7698 3375.8035 8453.9 1 4.923 26.9 1 K.IAKFERSEEEVTKLNVLVDEIKSQYYSR.I * Jamie_alpha_091208_22.08252.08252.3 4.5563 0.1692 100.0 3377.7122 3375.8035 7822.9 1 4.683 31.5 1 K.IAKFERSEEEVTKLNVLVDEIKSQYYSR.I * Jamie_alpha_091208_6.02651.02651.2 2.6282 0.1438 99.0 1453.7236 1453.5944 5100.3 2 4.139 70.0 1 R.VKEEYDIWQSR.D * Jamie_alpha_091208_8.03077.03077.4 5.2653 0.2841 100.0 3249.2354 3254.4075 7361.1 1 5.129 26.3 1 R.VKEEYDIWQSRDQGNDSLNDDLNKENK.L * Jamie_alpha_091208_7.03020.03020.3 4.8736 0.2382 100.0 3253.5288 3254.4075 8300.5 3 4.565 26.9 1 R.VKEEYDIWQSRDQGNDSLNDDLNKENK.L * Jamie_alpha_091208_12.04530.04530.3 4.5164 0.1125 98.0 3635.7703 3636.9138 6234.1 1 4.569 25.0 1 R.VKEEYDIWQSRDQGNDSLNDDLNKENKLLR.R * Jamie_alpha_091208_11.04463.04463.4 3.8169 0.2373 100.0 3635.7864 3636.9138 6514.3 2 4.672 22.4 1 R.VKEEYDIWQSRDQGNDSLNDDLNKENKLLR.R * Jamie_alpha_091208_7.02950.02950.2 3.6055 0.247 100.0 2202.0723 2202.3428 5079.1 1 4.969 50.0 1 R.DQGNDSLNDDLNKENKLLR.R * Jamie_alpha_091208_6.02619.02619.2 3.6353 0.2836 100.0 1906.0322 1906.146 6706.1 1 5.924 56.7 1 K.FLLNQNKQLSQSVEEK.V * Jamie_alpha_091208_10.03941.03941.3 2.776 0.1844 98.1 1649.8676 1648.9122 8069.3 1 4.269 40.4 1 K.QLSQSVEEKVLEMK.N * Jamie_alpha_091208_8.03357.03357.4 3.8813 0.209 100.0 2398.2341 2398.734 7698.8 1 4.774 32.5 1 K.NLKDTASVEKAEFSKEMTLQK.N * Jamie_alpha_091208_6.02408.02408.2 3.6761 0.356 100.0 1539.7784 1537.6764 7280.3 1 5.826 70.8 1 R.SQLTSLEKDCSLR.A * Jamie_alpha_091208_15.05765.05765.4 5.0237 0.2604 100.0 3682.8174 3682.9897 8042.2 7 5.061 21.1 1 R.AIEKNDDNSCRNPEHTDVIDELIDTKLRLEK.S * Jamie_alpha_091208_36.13040.13040.4 4.0486 0.1506 100.0 3432.7957 3431.9133 8469.1 1 4.519 24.7 1 R.NQKFQLQNQLEDFILELEHKTPELISFK.E * Jamie_alpha_091208_35.12783.12783.3 5.1038 0.4441 100.0 3434.834 3431.9133 9271.7 95 6.313 21.3 1 R.NQKFQLQNQLEDFILELEHKTPELISFK.E * Jamie_alpha_091208_37.13310.13310.5 4.3428 0.167 100.0 3346.75 3346.8076 5527.9 1 4.086 30.8 1 K.FQLQNQLEDFILELEHKTPELISFKER.T * Jamie_alpha_091208_36.13012.13012.4 3.7633 0.1723 100.0 3350.7708 3346.8076 5984.3 1 3.512 27.6 2 K.FQLQNQLEDFILELEHKTPELISFKER.T * Jamie_alpha_091208_1.00073.00073.2 2.0892 0.0679 93.1 1240.7018 1241.4331 7053.6 71 2.696 55.6 2 R.TKSLEHELKR.S * Jamie_alpha_091208_22.08211.08211.3 4.54 0.3158 100.0 3209.8074 3210.7393 9145.1 8 6.22 21.4 1 R.QVKLLLLNTSAIQETASPLSQDELISLRK.I * Jamie_alpha_091208_23.08657.08657.2 4.051 0.3389 100.0 2854.3123 2855.3018 7301.9 1 5.626 36.0 2 K.LLLLNTSAIQETASPLSQDELISLRK.I * Jamie_alpha_091208_23.08640.08640.3 4.3298 0.3958 100.0 2855.6033 2855.3018 7330.9 35 5.563 24.0 1 K.LLLLNTSAIQETASPLSQDELISLRK.I * Jamie_alpha_091208_24.09087.09087.2 3.5685 0.2905 100.0 2935.5723 2935.3018 3274.1 1 4.869 36.0 1 K.LLLLNTSAIQETAS*PLSQDELISLRK.I * Jamie_alpha_091208_24.09057.09057.3 5.3341 0.3057 100.0 2937.564 2935.3018 7737.3 3 6.679 29.0 1 K.LLLLNTSAIQETAS*PLSQDELISLRK.I * Jamie_alpha_091208_8.03358.03358.3 4.8287 0.1798 100.0 2373.227 2374.6104 7420.2 1 5.552 41.2 1 R.KILESSNIVNENDSQAIITER.L * Jamie_alpha_091208_8.03221.03221.2 5.6676 0.4341 100.0 2374.2297 2374.6104 7786.7 1 8.497 60.0 1 R.KILESSNIVNENDSQAIITER.L * Jamie_alpha_091208_10.03957.03957.3 4.9102 0.3374 100.0 2246.136 2246.4363 8011.4 1 5.669 44.7 1 K.ILESSNIVNENDSQAIITER.L * Jamie_alpha_091208_10.04127.04127.2 4.4462 0.2674 100.0 2247.1292 2246.4363 8261.0 1 5.417 55.3 1 K.ILESSNIVNENDSQAIITER.L * Jamie_alpha_091208_9.03434.03434.2 3.5338 0.3016 100.0 1550.7986 1549.721 7768.6 1 6.89 79.2 1 R.LVEFSNVNELQEK.N * Jamie_alpha_091208_20.07492.07492.3 6.4788 0.3735 100.0 2661.3823 2661.9812 6518.4 1 6.577 42.9 1 R.LVEFSNVNELQEKNVELLNCIR.I * Jamie_alpha_091208_12.04628.04628.1 3.4985 0.1704 100.0 1343.7228 1343.5199 5835.1 4 5.42 63.6 1 K.DAIIELENINAK.M * Jamie_alpha_091208_14.05238.05238.4 5.0451 0.3263 100.0 2875.898 2871.3071 6887.8 1 5.718 31.2 1 K.KIRELEAELSSTKVENSAIIQNLRK.E * Jamie_alpha_091208_11.04347.04347.3 5.1693 0.1783 100.0 2476.357 2473.7861 7777.3 1 5.422 41.7 1 R.ELEAELSSTKVENSAIIQNLRK.E * Jamie_alpha_091208_9.03681.03681.3 3.8923 0.1835 100.0 1872.0172 1870.1558 6258.4 1 4.608 40.0 1 K.KKTTLEDFENFKGLAK.E * Jamie_alpha_091208_11.04195.04195.3 4.1582 0.2499 100.0 1741.9202 1741.9817 5541.9 4 5.55 39.3 1 K.KTTLEDFENFKGLAK.E * Jamie_alpha_091208_10.03810.03810.2 3.776 0.3401 100.0 2155.1045 2156.4001 6131.9 1 5.991 52.9 1 K.TTLEDFENFKGLAKEKER.M * Jamie_alpha_091208_10.03801.03801.3 3.5851 0.2995 100.0 2156.106 2156.4001 5151.1 1 5.792 41.2 1 K.TTLEDFENFKGLAKEKER.M * Jamie_alpha_091208_17.06534.06534.4 2.4229 0.1824 93.3 2025.068 2026.3557 4556.7 12 4.112 37.5 1 R.MLEEAIDHLKAELEKQK.S * Jamie_alpha_091208_20.07499.07499.3 3.9312 0.1451 98.0 2902.5842 2900.3423 7129.3 1 4.598 32.3 1 K.SLEYEISKLKKETASFIPTKESLTR.D * Jamie_alpha_091208_14.05415.05415.3 3.9252 0.25 100.0 1915.0614 1916.185 7493.3 5 5.008 36.7 1 R.LRSDLQSKIQEIESIR.S * Jamie_alpha_091208_11.04177.04177.2 2.7177 0.1512 98.1 1708.3922 1706.931 6196.3 3 3.648 50.0 1 K.SLLTELSNKETTIEK.L * Jamie_alpha_091208_32.11531.11531.4 6.6048 0.3727 100.0 3240.0613 3234.6257 9080.2 1 6.742 36.4 1 K.SLLTELSNKETTIEKLSSEIENLDKELR.K * Jamie_alpha_091208_11.04356.04356.3 5.1158 0.313 100.0 2507.0942 2502.7864 8108.7 1 5.85 43.8 1 K.FQYKFLDQNSDASTLEPTLRK.E * Jamie_alpha_091208_7.02971.02971.2 4.6432 0.366 100.0 1937.9834 1936.1289 8266.6 1 5.94 53.1 2 K.FLDQNSDASTLEPTLRK.E * Jamie_alpha_091208_39.14110.14110.3 5.0744 0.2264 100.0 4458.321 4458.964 10361.4 1 5.732 24.3 1 K.ELEQIQVQLKDANSQIQAYEEIISSNENALIELKNELAK.T * Jamie_alpha_091208_3.01426.01426.3 3.013 0.1242 91.8 1967.0505 1967.2267 7588.0 32 4.465 36.7 1 K.TKENYDAKIELEKKEK.W * Jamie_alpha_091208_7.02841.02841.3 2.5233 0.2547 100.0 1761.8778 1762.917 4229.1 1 5.249 55.4 1 K.SSLYSAQDLLDKHER.K * Jamie_alpha_091208_7.02752.02752.2 4.4752 0.3576 100.0 1762.8793 1762.917 7555.6 1 7.895 75.0 1 K.SSLYSAQDLLDKHER.K * Jamie_alpha_091208_20.07450.07450.2 2.444 0.1701 98.1 2167.0322 2167.3372 5245.1 236 3.81 35.3 1 K.ADYERELISNIEQTESLR.V * Jamie_alpha_091208_15.05890.05890.3 4.2739 0.2258 100.0 2643.4443 2643.9163 8488.3 5 5.947 27.2 1 K.VDDTAANNGDKDHLKLVSLFSNLR.H * Jamie_alpha_091208_15.05599.05599.2 1.9364 0.0781 91.8 1051.6322 1049.2584 4678.8 6 3.508 68.8 1 K.LVSLFSNLR.H * Jamie_alpha_091208_11.04432.04432.2 4.99 0.4456 100.0 2490.3386 2490.819 6640.8 1 7.469 50.0 1 R.ELAFVKQKNDSLEKTINDLQR.T * Jamie_alpha_091208_22.08189.08189.3 5.2859 0.3182 100.0 2850.4143 2848.1426 9361.0 21 5.255 31.5 1 R.TQTLSEKEYQCSAVIIDEFKDITK.E * Jamie_alpha_091208_9.03579.03579.3 3.1618 0.1249 93.2 1955.1017 1955.2621 5109.4 1 4.369 40.6 1 K.EVTQVNILKENNAILQK.S * Jamie_alpha_091208_8.03380.03380.5 3.4534 0.1765 100.0 3095.6365 3092.4392 7708.1 184 3.88 22.9 1 K.SLKNVTEKNREIYKQLNDRQEEISR.L * Jamie_alpha_091208_12.04762.04762.4 3.6551 0.1756 100.0 3389.686 3393.776 6455.9 19 4.212 21.4 1 R.YQDLSQQQKDAQKKDIEKLTNEISDLKGK.L * Jamie_alpha_091208_21.07898.07898.4 5.569 0.2997 100.0 5268.6963 5268.7607 8939.7 8 5.198 15.2 1 R.YQDLSQQQKDAQKKDIEKLTNEISDLKGKLSSAENANADLENKFNR.L * Jamie_alpha_091208_3.00921.00921.2 2.2486 0.1931 99.0 1511.7922 1512.6627 8233.1 1 4.873 62.5 1 K.LKAHELQSEDVSR.D * Jamie_alpha_091208_20.07462.07462.6 3.7369 0.1562 100.0 4620.3564 4621.112 10165.0 1 3.716 20.8 1 K.KLQETLNKSTSSEAEYSKDIETLKKEWLKEYEDETLRR.I * Jamie_alpha_091208_16.06268.06268.3 3.3173 0.1626 98.1 2473.2559 2473.7393 8300.2 2 3.969 28.8 1 K.STSSEAEYSKDIETLKKEWLK.E * Jamie_alpha_091208_1.00050.00050.2 3.5055 0.116 100.0 1360.7883 1358.5809 8666.2 1 4.014 80.0 2 R.IKEAEENLKKR.I * Jamie_alpha_091208_16.06025.06025.3 3.8675 0.168 100.0 3346.5278 3347.487 5444.5 1 4.816 24.1 1 K.LKENAGSLTFLDNKGS*GEDAEEELWNSPSK.G * Jamie_alpha_091208_15.05919.05919.4 6.0339 0.3269 100.0 4924.404 4924.3057 8594.0 1 5.635 20.0 1 K.LKENAGSLTFLDNKGSGEDAEEELWNSPSKGNSERPSAVAGFINQK.N * Jamie_alpha_091208_16.06136.06136.4 3.7477 0.1735 100.0 5004.338 5004.3057 4327.7 224 3.49 14.4 1 K.LKENAGSLTFLDNKGSGEDAEEELWNS*PSKGNSERPSAVAGFINQK.N * Jamie_alpha_091208_8.03112.03112.2 3.721 0.2468 100.0 1734.7379 1735.7582 6962.5 1 5.176 70.0 1 K.GSGEDAEEELWNSPSK.G * Jamie_alpha_091208_8.03292.03292.2 4.1133 0.1756 100.0 1817.729 1815.7582 7546.1 12 5.022 53.3 2 K.GSGEDAEEELWNSPS*K.G * Jamie_alpha_091208_16.06234.06234.2 4.4342 0.4197 100.0 2838.379 2839.0508 8332.1 1 7.196 40.0 1 S.NTSSLQSFQNPFTASQSNINTNAPLR.T * Jamie_alpha_091208_13.05115.05115.2 3.5996 0.3347 100.0 2208.0767 2208.3953 8524.1 1 5.905 47.4 1 Q.SFQNPFTASQSNINTNAPLR.T * Jamie_alpha_091208_7.02913.02913.2 2.8547 0.1578 100.0 1211.699 1212.4319 4624.5 35 3.353 70.0 1 R.TLNIQPEVAVK.A * Jamie_alpha_091208_12.04643.04643.2 4.208 0.2059 100.0 2056.9722 2054.1777 8814.3 1 5.614 52.6 2 K.AAINFSNVTDLTNNSTDGAK.I * Jamie_alpha_091208_14.05397.05397.3 4.7442 0.3355 100.0 3999.9932 4001.264 7787.1 1 5.7 21.1 1 K.AAINFSNVTDLTNNSTDGAKITEIGSTSKRPIESGTSSD.P * Jamie_alpha_091208_12.04534.04534.2 5.4687 0.5245 100.0 1981.9436 1983.099 8466.0 1 8.912 66.7 1 A.AINFSNVTDLTNNSTDGAK.I * Jamie_alpha_091208_11.04190.04190.2 3.8092 0.4126 100.0 1911.903 1912.0203 6743.7 1 6.843 47.1 1 A.INFSNVTDLTNNSTDGAK.I YLR200W 1 1 49.1% 114 13284 4.7 U YKE2 SGDID:S000004190, Chr XII from 549014-549358, Verified ORF, "Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin" * Jamie_alpha_091208_64.22269.22269.4 3.1855 0.2146 100.0 6822.2163 6827.3887 9348.6 6 4.155 13.0 1 K.IVNEEFDQLEEDTPVY*KLT*GNVLLPVEQSEARTNVDKRLEFIETEITRCEKNIRDK.Q YOR382W 1 1 41.8% 153 15302 8.4 U FIT2 SGDID:S000005909, Chr XV from 1059527-1059988, Verified ORF, "Mannoprotein that is incorporated into the cell wall via a glycosylphosphatidylinositol (GPI) anchor, involved in the retention of siderophore-iron in the cell wall" * Jamie_alpha_091208_64.22380.22380.4 2.8544 0.4391 100.0 6240.124 6244.4116 7600.9 198 2.897 11.9 1 S.EKSTTKTLTLTNGSGSSTNLYT*KTVTQAVESSTSSSS*SSSSSSSSASSSGAAPAAFQGASVGAL.A YLR068W 1 1 26.5% 151 18190 10.3 U FYV7 SGDID:S000004058, Chr XII from 271009-271464, Verified ORF, "Protein of unknown function, required for survival upon exposure to K1 killer toxin; involved in processing the 35S rRNA primary transcript to generate the 20S and 27SA2 pre-rRNA transcripts" * Jamie_alpha_091208_47.16805.16805.4 4.1603 0.1303 100.0 4720.7017 4720.338 7463.4 288 3.459 16.7 1 R.KEYLKALKDEGY*AVPEKEPKTVAKES*VRKIKEARAIEGKK.K contaminant_KERATIN02 9 10 25.4% 622 61987 5.2 U no description * Jamie_alpha_091208_2.00799.00799.2 2.9116 0.3654 100.0 1232.5951 1233.2833 6949.0 2 7.077 53.3 1 T.SGGGGGGGLGSGGSIR.S * Jamie_alpha_091208_15.05723.05723.2 4.8639 0.5495 100.0 2705.1494 2706.7605 9105.6 1 10.614 35.5 1 R.GGGGSFGYSYGGGSGGGFSASSLGGGFGGGSR.G * Jamie_alpha_091208_25.09395.09395.4 4.6567 0.2125 100.0 3454.7314 3455.8027 8821.4 5 4.573 24.4 1 R.LASYLDKVQALEEANNDLENKIQDWYDKK.G * Jamie_alpha_091208_17.06516.06516.3 4.9011 0.317 100.0 3663.7458 3664.8784 7826.6 1 5.463 28.2 1 K.NRKDIENQYETQITQIEHEVSSSGQEVQSSAK.E * Jamie_alpha_091208_23.08518.08518.4 5.6589 0.2416 100.0 4392.168 4391.7095 8188.2 2 5.449 19.4 1 K.NRKDIENQYETQITQIEHEVSSSGQEVQSSAKEVTQLR.H * Jamie_alpha_091208_16.06171.06171.2 5.3856 0.3562 100.0 1837.9633 1839.0557 7113.1 1 8.856 76.7 1 R.HGVQELEIELQSQLSK.K * Jamie_alpha_091208_14.05313.05313.2 3.9762 0.391 100.0 1969.3121 1967.2297 9333.5 1 6.681 56.2 1 R.HGVQELEIELQSQLSKK.A * Jamie_alpha_091208_1.00139.00139.2 3.9485 0.2722 100.0 2094.9023 2093.05 6195.6 1 7.361 38.0 2 R.GSRGGSGGSYGGGGSGGGYGGGSGSR.G * Jamie_alpha_091208_2.00470.00470.2 5.1178 0.4158 100.0 1792.7305 1792.7324 8022.9 1 9.677 47.7 1 R.GGSGGSYGGGGSGGGYGGGSGSR.G YBL003C 1 1 22.0% 132 13989 10.7 U HTA2 SGDID:S000000099, Chr II from 235795-235397, reverse complement, Verified ORF, "One of two nearly identical (see also HTA1) histone H2A subtypes; core histone required for chromatin assembly and chromosome function; DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p " YDR225W 1 1 22.0% 132 13989 10.7 U HTA1 SGDID:S000002633, Chr IV from 915524-915922, Verified ORF, "One of two nearly identical (see also HTA2) histone H2A subtypes; core histone required for chromatin assembly and chromosome function; DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p " Jamie_alpha_091208_61.21476.21476.2 3.1053 0.3121 100.0 2947.4758 2947.4014 9677.4 1 5.774 33.9 1 R.IGSGAPVYLTAVLEYLAAEILELAGNAAR.D YPL037C 1 1 19.1% 157 17020 6.5 U EGD1 SGDID:S000005958, Chr XVI from 481898-481425, reverse complement, Verified ORF, "Subunit beta1 of the nascent polypeptide-associated complex (NAC) involved in protein targeting, associated with cytoplasmic ribosomes; enhances DNA binding of the Gal4p activator; homolog of human BTF3b" * Jamie_alpha_091208_58.20164.20164.2 2.7501 0.2186 100.0 3227.331 3225.7278 10643.7 3 5.145 27.6 1 K.NLQDLFPGIISQLGPEAIQALSQLAAQMEK.H contaminant_NRL_1IKFL 2 2 18.7% 214 23527 6.5 U owl|| Immunoglobulin igg1-kappa antibody fragment fab complexed With... contaminant_S68241 2 2 18.3% 218 23846 6.7 U owl|S68241| immunoglobulin light chain (Mab13-1) - mouse (fragment) Jamie_alpha_091208_32.11717.11717.3 3.9115 0.1508 97.9 4301.1846 4299.793 7613.3 1 4.244 18.6 1 L.RADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVK.W Jamie_alpha_091208_39.13842.13842.2 2.5871 0.1797 98.9 3573.7322 3573.947 10424.7 2 4.048 21.2 1 R.ADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPK.D Reverse_YJL122W 1 1 16.6% 175 19287 10.0 U YJL122W SGDID:S000003658, Chr X from 189636-190163, Uncharacterized ORF, "Hypothetical protein" * Jamie_alpha_091208_72.24914.24914.4 3.1952 0.1459 90.6 3182.2546 3183.2842 5433.9 180 3.506 22.6 1 K.S*LTRTTIS*APSSSANQLTVATSNQSENKK.N Reverse_YBR164C 1 1 16.4% 183 20434 4.9 U ARL1 SGDID:S000000368, Chr II from 568421-567870, reverse complement, Verified ORF, "Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor" * Jamie_alpha_091208_19.07142.07142.2 1.569 0.1863 94.8 3367.2922 3364.8591 7072.6 44 3.47 22.4 1 K.GAGDLGLILIRLEKNSGWLKDFMSS*FINGM.- YDL229W 6 6 16.0% 613 66602 5.4 U SSB1 SGDID:S000002388, Chr IV from 44066-45907, Verified ORF, "Cytoplasmic ATPase that is a ribosome-associated molecular chaperone; may be involved in the folding of newly-synthesized polypeptide chains; member of the heat shock protein 70 (HSP70) family; interacts with the phosphatase subunit Reg1p" Jamie_alpha_091208_12.04601.04601.2 3.0865 0.3335 100.0 1395.755 1395.6011 7870.8 12 5.717 63.6 1 R.ARFEDLNAALFK.S Jamie_alpha_091208_9.03487.03487.3 2.7301 0.2487 100.0 1788.9852 1789.0391 5876.4 1 4.99 39.1 1 K.ISKSQIDEVVLVGGSTR.I Jamie_alpha_091208_14.05342.05342.2 2.3441 0.1788 99.0 1542.0809 1540.7563 6821.5 7 3.93 54.2 1 K.LLSDFFDGKQLEK.S * Jamie_alpha_091208_12.04563.04563.2 4.7199 0.3261 100.0 2465.1306 2464.6167 8542.6 1 6.914 50.0 1 R.TFTTCADNQTTVQFPVYQGER.V Jamie_alpha_091208_13.05112.05112.2 3.6645 0.1526 100.0 1782.8943 1780.9818 7759.9 1 4.041 60.7 1 R.VNCKENTLLGEFDLK.N Jamie_alpha_091208_11.04470.04470.2 2.6148 0.2048 100.0 2133.5122 2135.336 5729.9 74 4.66 31.6 1 K.SSNITISNAVGRLSSEEIEK.M YNL209W 6 6 16.0% 613 66595 5.5 U SSB2 SGDID:S000005153, Chr XIV from 252060-253901, Verified ORF, "Cytoplasmic ATPase that is a ribosome-associated molecular chaperone; may be involved in the folding of newly-synthesized polypeptide chains; member of the heat shock protein 70 (HSP70) family; homolog of SSB1" Jamie_alpha_091208_12.04601.04601.2 3.0865 0.3335 100.0 1395.755 1395.6011 7870.8 12 5.717 63.6 1 R.ARFEDLNAALFK.S Jamie_alpha_091208_9.03487.03487.3 2.7301 0.2487 100.0 1788.9852 1789.0391 5876.4 1 4.99 39.1 1 K.ISKSQIDEVVLVGGSTR.I Jamie_alpha_091208_14.05342.05342.2 2.3441 0.1788 99.0 1542.0809 1540.7563 6821.5 7 3.93 54.2 1 K.LLSDFFDGKQLEK.S * Jamie_alpha_091208_13.04884.04884.2 4.3495 0.3381 100.0 2419.15 2419.6096 7800.2 1 6.479 42.5 1 R.TFTTVSDNQTTVQFPVYQGER.V Jamie_alpha_091208_13.05112.05112.2 3.6645 0.1526 100.0 1782.8943 1780.9818 7759.9 1 4.041 60.7 1 R.VNCKENTLLGEFDLK.N Jamie_alpha_091208_11.04470.04470.2 2.6148 0.2048 100.0 2133.5122 2135.336 5729.9 74 4.66 31.6 1 K.SSNITISNAVGRLSSEEIEK.M contaminant_INT-STD1 8 10 15.5% 607 69271 6.1 U BSA * Jamie_alpha_091208_1.00047.00047.2 1.9054 0.1686 96.6 1193.6044 1194.2925 6983.4 10 3.842 66.7 1 R.DTHKSEIAHR.F * Jamie_alpha_091208_9.03709.03709.3 4.092 0.3481 100.0 2867.434 2866.009 7802.9 1 6.115 29.2 1 K.TCVADESHAGCEKSLHTLFGDELCK.V * Jamie_alpha_091208_10.04111.04111.2 3.5607 0.3841 100.0 1422.7028 1420.5713 4939.6 1 6.55 77.3 1 K.SLHTLFGDELCK.V * Jamie_alpha_091208_22.08373.08373.2 3.9326 0.4022 100.0 1691.8646 1693.9781 8812.9 1 6.564 71.4 2 K.AEFVEVTKLVTDLTK.V * Jamie_alpha_091208_9.03753.03753.3 4.0125 0.0951 100.0 1439.8107 1440.6884 5041.4 1 4.92 70.5 2 R.RHPEYAVSVLLR.L * Jamie_alpha_091208_6.02627.02627.2 1.8142 0.1856 97.5 1305.0007 1306.5046 5453.4 4 5.06 65.0 1 K.HLVDEPQNLIK.Q * Jamie_alpha_091208_24.08950.08950.3 5.6254 0.4119 100.0 3818.9194 3818.2468 10140.0 1 6.766 26.6 1 K.HLVDEPQNLIKQNCDQFEKLGEYGFQNALIVR.Y * Jamie_alpha_091208_15.05615.05615.2 3.201 0.2167 100.0 1479.7987 1480.7068 10021.3 2 5.003 62.5 1 K.LGEYGFQNALIVR.Y YPL131W 2 2 15.2% 297 33715 6.8 U RPL5 SGDID:S000006052, Chr XVI from 303120-304013, Verified ORF, "Protein component of the large (60S) ribosomal subunit with similarity to E. coli L18 and rat L5 ribosomal proteins; binds 5S rRNA and is required for 60S subunit assembly" * Jamie_alpha_091208_18.07050.07050.2 3.9702 0.3166 100.0 2581.216 2580.7644 7166.8 1 6.019 38.6 1 K.GVEEVEGEYELTEAVEDGPRPFK.V * Jamie_alpha_091208_20.07608.07608.2 3.2126 0.2815 100.0 2592.9321 2591.7217 6358.1 6 4.721 33.3 1 R.SYIFGGHVSQYMEELADDDEER.F YDL130W 1 1 15.1% 106 10668 4.0 U RPP1B SGDID:S000002288, Chr IV from 229906-230019,230321-230527, Verified ORF, "Ribosomal protein P1 beta, component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; accumulation is regulated by phosphorylation and interaction with the P2 stalk component" * Jamie_alpha_091208_13.05011.05011.2 4.2554 0.4284 100.0 1664.8102 1664.815 5977.2 1 7.445 63.3 1 K.AAGANVDNVWADVYAK.A YAL038W 6 6 15.0% 500 54545 7.7 U CDC19 SGDID:S000000036, Chr I from 71787-73289, Verified ORF, "Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration" * Jamie_alpha_091208_11.04484.04484.2 3.2792 0.1609 100.0 2186.1777 2186.4705 7646.8 2 4.657 44.7 1 R.TANDVLTIREVLGEQGKDVK.I * Jamie_alpha_091208_15.05851.05851.2 2.7297 0.1216 98.2 1878.0922 1878.2194 6397.4 12 3.808 41.2 1 R.GDLGIEIPAPEVLAVQKK.L * Jamie_alpha_091208_25.09235.09235.3 4.2154 0.2795 100.0 2571.8044 2570.8174 7835.8 1 5.219 38.1 1 R.GVFPFVFEKEPVSDWTDDVEAR.I * Jamie_alpha_091208_25.09396.09396.2 4.9956 0.3773 100.0 2572.2214 2570.8174 6432.1 1 6.592 54.8 1 R.GVFPFVFEKEPVSDWTDDVEAR.I * Jamie_alpha_091208_8.03336.03336.2 2.4446 0.3528 100.0 1520.6727 1519.5646 7308.5 22 5.703 50.0 1 K.EPVSDWTDDVEAR.I * Jamie_alpha_091208_13.05145.05145.2 2.4461 0.1076 95.3 1709.2122 1708.0538 6359.3 1 3.291 57.1 1 R.INFGIEKAKEFGILK.K YGR282C 4 4 15.0% 313 34119 4.5 U BGL2 SGDID:S000003514, Chr VII from 1058730-1057789, reverse complement, Verified ORF, "Endo-beta-1,3-glucanase, major protein of the cell wall, involved in cell wall maintenance" * Jamie_alpha_091208_15.05667.05667.2 2.7825 0.2624 100.0 1162.9722 1161.3867 7003.1 1 5.017 75.0 1 A.IGELAFNLGVK.N * Jamie_alpha_091208_10.03915.03915.2 4.3971 0.5178 100.0 1951.977 1951.1528 7035.5 1 8.81 55.9 1 A.IGELAFNLGVKNNDGTCK.S * Jamie_alpha_091208_8.03330.03330.2 4.0265 0.3542 100.0 2275.0112 2275.354 5702.5 1 6.045 52.6 1 K.NNDGTCKSTSDYETELQALK.S * Jamie_alpha_091208_7.03009.03009.3 3.8491 0.33 100.0 1788.912 1789.9402 5477.7 6 5.735 35.0 1 R.NDLTASQLSDKINDVR.S contaminant_KERATIN13 6 6 14.5% 643 65494 6.6 U no description * Jamie_alpha_091208_15.05888.05888.4 3.9513 0.2895 100.0 2423.2874 2423.7341 7077.5 1 5.647 35.1 1 R.EREQIKSLNNQFASFIDKVR.F * Jamie_alpha_091208_16.06223.06223.3 2.1013 0.2019 92.3 1638.8582 1639.8516 3106.1 69 4.885 42.3 1 K.SLNNQFASFIDKVR.F * Jamie_alpha_091208_19.07294.07294.3 5.9232 0.4135 100.0 2935.5054 2934.2786 10345.9 1 7.393 34.8 1 R.FLEQQNQVLQTKWELLQQVDTSTR.T * Jamie_alpha_091208_25.09273.09273.2 3.4827 0.3061 100.0 1996.0149 1995.2017 5336.0 2 5.873 50.0 1 R.THNLEPYFESFINNLR.R * Jamie_alpha_091208_7.02854.02854.2 2.2484 0.1719 98.1 1267.6635 1266.3934 5783.0 11 3.939 65.0 1 R.TNAENEFVTIK.K * Jamie_alpha_091208_9.03499.03499.2 4.833 0.3341 100.0 2502.2546 2502.7405 9530.9 1 6.931 52.4 1 K.SKAEAESLYQSKYEELQITAGR.H YER011W 1 1 14.2% 254 24906 4.8 U TIR1 SGDID:S000000813, Chr V from 175247-176011, Verified ORF, "Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expression is downregulated at acidic pH and induced by cold shock and anaerobiosis; abundance is increased in cells cultured without shaking" * Jamie_alpha_091208_69.23891.23891.3 3.1912 0.1586 94.6 3450.718 3449.3586 6248.1 38 3.713 17.9 1 K.SSSAAPSSSEAKSSSAAPSS*SEAKS*SSAAPSSSEAK.S YDR432W 3 3 12.6% 414 45407 5.5 U NPL3 SGDID:S000002840, Chr IV from 1328773-1330017, Verified ORF, "RNA-binding protein that carries poly(A)+ mRNA from the nucleus into the cytoplasm; phosphorylation by Sky1p in the cytoplasm may promote release of mRNAs" * Jamie_alpha_091208_13.05109.05109.2 2.7074 0.0837 95.3 1901.915 1903.068 6224.3 11 4.349 46.7 1 K.NLPEGCSWQDLKDLAR.E * Jamie_alpha_091208_9.03454.03454.2 2.5265 0.1643 99.0 1585.7532 1585.6677 8284.9 8 4.584 53.8 1 R.ENSLETTFSSVNTR.D * Jamie_alpha_091208_37.13440.13440.2 2.9629 0.1414 98.9 2440.1777 2438.6501 9460.2 20 4.338 31.0 1 R.DFDGTGALEFPSEEILVEALER.L contaminant_KERATIN03 4 4 12.5% 593 59519 5.2 U no description * Jamie_alpha_091208_35.12786.12786.3 4.2964 0.1893 100.0 3053.6465 3054.4277 9682.6 137 4.822 23.1 1 K.TIDDLKNQILNLTTDNANILLQIDNAR.L * Jamie_alpha_091208_9.03630.03630.2 2.074 0.1243 96.7 1109.494 1110.1681 5536.1 16 4.66 62.5 1 K.DAEAWFNEK.S * Jamie_alpha_091208_18.07048.07048.4 4.8904 0.2563 100.0 3198.6401 3198.5115 6223.8 2 5.473 29.5 1 K.SKELTTEIDNNIEQISSYKSEITELRR.N * Jamie_alpha_091208_6.02575.02575.2 2.8384 0.1376 98.9 1434.7665 1435.623 7903.6 1 4.693 75.0 1 K.IRLENEIQTYR.S YOL127W 1 1 12.0% 142 15758 10.1 U RPL25 SGDID:S000005487, Chr XV from 80347-80359,80774-81189, Verified ORF, "Primary rRNA-binding ribosomal protein component of the large (60S) ribosomal subunit, has similarity to E. coli L23 and rat L23a ribosomal proteins; binds to 26S rRNA via a conserved C-terminal motif" * Jamie_alpha_091208_25.09307.09307.2 2.169 0.1223 95.5 1896.9253 1898.1228 7611.3 168 3.591 34.4 1 R.LTADYDALDIANRIGYI.- YOR369C 1 1 11.9% 143 15472 4.7 U RPS12 SGDID:S000005896, Chr XV from 1028621-1028190, reverse complement, Verified ORF, "Protein component of the small (40S) ribosomal subunit; has similarity to rat ribosomal protein S12" * Jamie_alpha_091208_9.03643.03643.2 3.7984 0.1839 100.0 1852.0322 1850.1655 7870.9 1 4.689 62.5 1 K.LVEGLANDPENKVPLIK.V YGR254W 3 3 10.8% 437 46816 6.6 U ENO1 SGDID:S000003486, Chr VII from 1000932-1002245, Verified ORF, "Enolase I, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression is repressed in response to glucose" YHR174W 3 3 10.8% 437 46914 6.0 U ENO2 SGDID:S000001217, Chr VIII from 451327-452640, Verified ORF, "Enolase II, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression is induced in response to glucose" Jamie_alpha_091208_11.04456.04456.3 3.9364 0.1164 98.2 2583.3237 2584.8455 8903.9 1 3.63 29.5 1 R.SVYDSRGNPTVEVELTTEKGVFR.S Jamie_alpha_091208_32.11754.11754.3 6.1635 0.438 100.0 2988.4177 2986.269 7398.4 1 7.483 37.0 1 K.RYPIVSIEDPFAEDDWEAWSHFFK.T Jamie_alpha_091208_37.13161.13161.3 5.3652 0.4558 100.0 2831.2986 2830.0815 6239.8 1 7.837 47.7 1 R.YPIVSIEDPFAEDDWEAWSHFFK.T Reverse_YDR494W 1 1 9.7% 361 41216 10.0 U RSM28 SGDID:S000002902, Chr IV from 1436920-1438005, Verified ORF, "Mitochondrial ribosomal protein of the small subunit; genetic interactions suggest a possible role in promoting translation initiation" * Jamie_alpha_091208_39.13993.13993.3 3.2827 0.1551 94.5 3938.399 3941.174 10850.5 57 4.243 19.1 1 K.DLKLANSSAKAT*AEQARALISNIFDETVSTSY*HAR.S YAL005C 3 4 9.3% 642 69768 5.1 U SSA1 SGDID:S000000004, Chr I from 141433-139505, reverse complement, Verified ORF, "ATPase involved in protein folding and nuclear localization signal (NLS)-directed nuclear transport; member of heat shock protein 70 (HSP70) family; forms a chaperone complex with Ydj1p; localized to the nucleus, cytoplasm, and cell wall" YLL024C 3 4 9.4% 639 69470 5.1 U SSA2 SGDID:S000003947, Chr XII from 97484-95565, reverse complement, Verified ORF, "ATP binding protein involved in protein folding and vacuolar import of proteins; member of heat shock protein 70 (HSP70) family; associated with the chaperonin-containing T-complex; present in the cytoplasm, vacuolar membrane and cell wall" Jamie_alpha_091208_12.04703.04703.2 3.9625 0.373 100.0 1897.9922 1896.0667 3951.1 1 6.181 53.1 1 K.VNDAVVTVPAYFNDSQR.Q Jamie_alpha_091208_15.05617.05617.2 3.3387 0.2805 100.0 1529.7126 1527.6862 7252.1 2 5.448 72.7 2 R.ARFEELCADLFR.S Jamie_alpha_091208_21.07921.07921.3 3.2983 0.1211 92.2 3373.0444 3369.8406 7283.8 21 3.641 21.7 1 R.STLDPVEKVLRDAKLDKSQVDEIVLVGGSTR.I Reverse_YHR089C 1 1 9.3% 205 21480 11.5 U GAR1 SGDID:S000001131, Chr VIII from 283300-282683, reverse complement, Verified ORF, "Protein component of the H/ACA snoRNP pseudouridylase complex, involved in the modification and cleavage of the 18S pre-rRNA" * Jamie_alpha_091208_58.20168.20168.2 2.0881 0.1678 96.6 1845.9305 1845.133 6241.2 116 4.258 33.3 1 R.GMSAGGRGGPAGSRKKKNK.P YOR257W 1 1 9.3% 161 18751 4.6 U CDC31 SGDID:S000005783, Chr XV from 811006-811491, Verified ORF, "Component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; interacts with Kar1p" * Jamie_alpha_091208_7.02986.02986.3 3.0839 0.3184 100.0 1702.92 1703.8871 5387.8 14 5.736 42.9 1 R.VAKELGETLTDEELR.A contaminant_gi|135017|sp|P07518|SUBT_BAC 3 4 9.1% 275 27656 6.8 U SUBTILISIN (ALKALINE MESENTERICOPEPTIDASE) * Jamie_alpha_091208_3.01385.01385.3 3.2578 0.2822 100.0 1529.7734 1530.6805 5662.1 2 6.201 44.6 2 K.APALHSQGYTGSNVK.V * Jamie_alpha_091208_3.01316.01316.2 3.2913 0.398 100.0 1532.7722 1530.6805 6807.8 1 6.622 60.7 1 K.APALHSQGYTGSNVK.V * Jamie_alpha_091208_8.03157.03157.2 2.1418 0.0492 90.2 984.5461 985.12823 5855.2 356 3.596 55.6 1 K.GLINVQAAAQ.- YAL012W 2 2 8.9% 394 42542 6.5 U CYS3 SGDID:S000000010, Chr I from 130802-131986, Verified ORF, "Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine;" * Jamie_alpha_091208_11.04431.04431.2 2.0931 0.1996 99.0 1207.5953 1208.3135 5752.6 13 4.314 65.0 1 R.EASGVFDDLVR.I * Jamie_alpha_091208_37.13343.13343.3 3.7437 0.1949 100.0 2633.3823 2630.9092 7307.4 20 4.665 30.4 1 R.ISVGIEDTDDLLEDIKQALKQATN.- YKL152C 1 1 8.9% 247 27609 8.8 U GPM1 SGDID:S000001635, Chr XI from 164390-163647, reverse complement, Verified ORF, "Tetrameric phosphoglycerate mutase, mediates the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis and the reverse reaction during gluconeogenesis" * Jamie_alpha_091208_12.04852.04852.2 2.7469 0.155 99.0 2374.152 2374.5688 4512.9 3 5.045 38.1 1 R.SFDVPPPPIDASSPFSQKGDER.Y YGR192C 2 2 8.7% 332 35747 7.0 U TDH3 SGDID:S000003424, Chr VII from 883815-882817, reverse complement, Verified ORF, "Glyceraldehyde-3-phosphate dehydrogenase, isozyme 3, involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell-wall " YJR009C 2 2 8.7% 332 35847 7.0 U TDH2 SGDID:S000003769, Chr X from 454595-453597, reverse complement, Verified ORF, "Glyceraldehyde-3-phosphate dehydrogenase, isozyme 2, involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell-wall " YJL052W 2 2 8.7% 332 35750 8.3 U TDH1 SGDID:S000003588, Chr X from 338187-339185, Verified ORF, "Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1, involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell-wall " Jamie_alpha_091208_7.02692.02692.2 2.8638 0.1964 100.0 1822.9785 1821.0131 8061.4 1 4.366 59.4 1 K.IVSNASCTTNCLAPLAK.V Jamie_alpha_091208_6.02395.02395.2 2.2788 0.1131 96.0 1238.7446 1239.5009 7462.7 10 3.484 59.1 1 K.AVGKVLPELQGK.L YCL043C 2 2 7.9% 522 58227 4.5 U PDI1 SGDID:S000000548, Chr III from 50221-48653, reverse complement, Verified ORF, "Protein disulfide isomerase, multifunctional protein resident in the endoplasmic reticulum lumen, essential for the formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds" * Jamie_alpha_091208_21.08084.08084.3 3.7301 0.0848 91.7 2031.1567 2031.4001 10672.3 39 4.251 33.3 1 K.AIESLVKDFLKGDASPIVK.S * Jamie_alpha_091208_17.06690.06690.2 4.5994 0.394 100.0 2343.9722 2343.33 7381.7 1 7.103 57.1 1 K.AAEEADADAELADEEDAIHDEL.- YLR407W 1 1 7.9% 229 25687 8.6 U YLR407W SGDID:S000004399, Chr XII from 932965-933654, Uncharacterized ORF, "Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery" * Jamie_alpha_091208_69.23864.23864.2 2.2543 0.0699 91.8 2174.4756 2176.536 5258.6 168 3.742 32.4 1 K.LAQKSKAPFCNAEKIWKR.R YKL096W 1 1 7.9% 239 24268 4.7 U CWP1 SGDID:S000001579, Chr XI from 260776-261495, Verified ORF, "Cell wall mannoprotein, linked to a beta-1,3- and beta-1,6-glucan heteropolymer through a phosphodiester bond; involved in cell wall organization" * Jamie_alpha_091208_13.04955.04955.2 4.2326 0.4049 100.0 2033.9722 2032.214 7611.6 1 7.934 55.6 1 K.SSSGFYAIKDGSSYIFSSK.Q YLR044C 2 2 7.8% 563 61495 6.2 U PDC1 SGDID:S000004034, Chr XII from 234082-232391, reverse complement, Verified ORF, "Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde; subject to glucose-, ethanol-, and autoregulation; involved in amino acid catabolism" Jamie_alpha_091208_10.03949.03949.2 4.8712 0.4524 100.0 2000.9692 1999.1063 10527.7 1 8.992 52.8 1 R.WAGNANELNAAYAADGYAR.I * Jamie_alpha_091208_21.07909.07909.2 3.5628 0.403 100.0 2742.508 2744.2065 6810.1 1 7.18 39.6 1 R.TTYVTQRPVYLGLPANLVDLNVPAK.L YPR148C 1 1 7.8% 435 49279 4.8 U YPR148C SGDID:S000006352, Chr XVI from 828136-826829, reverse complement, Uncharacterized ORF, "Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern" * Jamie_alpha_091208_11.04248.04248.3 4.846 0.3032 100.0 3865.5842 3865.7427 7951.2 1 4.82 22.7 1 K.TAQTTEAQGADHNEEDEEDEEDEEDDEDLSNLIK.V YGR234W 1 1 7.8% 399 44646 6.3 U YHB1 SGDID:S000003466, Chr VII from 959908-961107, Verified ORF, "Nitric oxide oxidoreductase, flavohemoglobin involved in nitric oxide detoxification; plays a role in the oxidative and nitrosative stress responses" * Jamie_alpha_091208_59.20602.20602.2 4.3496 0.4561 100.0 3348.703 3349.762 7749.5 1 8.012 31.7 1 K.EVLGDAATPEIINAWGEAYQAIADIFITVEK.K YEL034W 1 1 7.6% 157 17114 5.0 U HYP2 SGDID:S000000760, Chr V from 85676-86149, Verified ORF, "Translation initiation factor eIF-5A, promotes formation of the first peptide bond; similar to and functionally redundant with Anb1p; undergoes an essential hypusination modification; expressed under aerobic conditions" * Jamie_alpha_091208_16.06224.06224.2 2.4199 0.1821 98.9 1312.7621 1313.5828 7137.3 14 4.698 54.5 1 K.VHLVAIDIFTGK.K YLR134W 2 2 7.5% 563 61912 6.4 U PDC5 SGDID:S000004124, Chr XII from 410724-412415, Verified ORF, "Minor isoform of pyruvate decarboxylase, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde, regulation is glucose- and ethanol-dependent, repressed by thiamine, involved in amino acid catabolism" Jamie_alpha_091208_10.03949.03949.2 4.8712 0.4524 100.0 2000.9692 1999.1063 10527.7 1 8.992 52.8 1 R.WAGNANELNAAYAADGYAR.I * Jamie_alpha_091208_39.13948.13948.2 2.6389 0.3461 100.0 2325.465 2322.6262 8359.9 1 5.847 34.1 1 A.GSYAEHVGVLHVVGVPSISSQAK.Q YHR203C 1 1 7.3% 261 29410 10.1 U RPS4B SGDID:S000001246, Chr VIII from 505530-505517,505247-504476, reverse complement, Verified ORF, "Protein component of the small (40S) ribosomal subunit; identical to Rps4Bp and has similarity to rat S4 ribosomal protein" YJR145C 1 1 7.3% 261 29410 10.1 U RPS4A SGDID:S000003906, Chr X from 702983-702970,702713-701942, reverse complement, Verified ORF, "Protein component of the small (40S) ribosomal subunit; mutation affects 20S pre-rRNA processing; identical to Rps4Bp and has similarity to rat S4 ribosomal protein" Jamie_alpha_091208_16.06325.06325.2 2.908 0.0064 90.0 2120.152 2117.496 9017.8 2 3.147 41.7 1 R.LNNVFVIGEQGKPYISLPK.G YER091C 2 2 7.2% 767 85860 6.5 U MET6 SGDID:S000000893, Chr V from 342163-339860, reverse complement, Verified ORF, "Cobalamin-independent methionine synthase, involved in amino acid biosynthesis; requires a minimum of two glutamates on the methyltetrahydrofolate substrate, similar to bacterial metE homologs" * Jamie_alpha_091208_52.18445.18445.2 3.1831 0.2738 100.0 3513.2764 3513.9258 12629.9 14 5.485 23.3 1 K.EAGVDIIPSNDFSFYDQVLDLSLLFNVIPDR.Y * Jamie_alpha_091208_59.20664.20664.2 2.2537 0.1132 95.5 2742.8657 2744.195 6745.5 84 3.435 26.1 1 K.DSLDLEPLSLLEQLLPLYTEILSK.L YBR127C 2 2 7.2% 517 57749 5.1 U VMA2 SGDID:S000000331, Chr II from 492816-491263, reverse complement, Verified ORF, "Subunit B of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; contains nucleotide binding sites; also detected in the cytoplasm" * Jamie_alpha_091208_18.06892.06892.2 2.5721 0.1622 98.1 1930.0686 1931.2395 7607.6 128 3.961 35.3 1 R.LNYNTVSGVNGPLVILEK.V * Jamie_alpha_091208_20.07616.07616.2 3.774 0.2831 100.0 2157.2212 2155.3716 8360.1 1 5.981 50.0 1 K.VFAEDYLDINGSPINPYAR.I YMR131C 1 1 7.2% 511 57261 4.6 U RRB1 SGDID:S000004738, Chr XIII from 534697-533162, reverse complement, Verified ORF, "Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with the ribosomal protein Rpl3p" * Jamie_alpha_091208_12.04666.04666.4 4.4676 0.2639 100.0 4287.9365 4289.3447 8083.4 1 4.507 20.8 1 K.TLLKDDNEGEDDEEDDEDDVDPVIENENIPLRDTTNR.L YLR196W 1 1 7.1% 576 63803 4.6 U PWP1 SGDID:S000004186, Chr XII from 543970-545700, Verified ORF, "Protein with WD-40 repeats involved in rRNA processing; associates with trans-acting ribosome biogenesis factors; similar to beta-transducin superfamily" * Jamie_alpha_091208_20.07571.07571.3 3.0675 0.2227 100.0 4754.1245 4756.004 5876.0 108 4.018 15.6 1 K.YWSAMAGEEIETVTFASENIILCGTDS*GNVYSFDIRNNENR.K YGL031C 1 1 7.1% 155 17614 11.3 U RPL24A SGDID:S000002999, Chr VII from 437939-437472, reverse complement, Verified ORF, "Ribosomal protein L30 of the large (60S) ribosomal subunit, nearly identical to Rpl24Bp and has similarity to rat L24 ribosomal protein; not essential for translation but may be required for normal translation rate" YGR148C 1 1 7.1% 155 17547 11.4 U RPL24B SGDID:S000003380, Chr VII from 787784-787317, reverse complement, Verified ORF, "Ribosomal protein L30 of the large (60S) ribosomal subunit, nearly identical to Rpl24Ap and has similarity to rat L24 ribosomal protein; not essential for translation but may be required for normal translation rate" Jamie_alpha_091208_2.00774.00774.2 2.1115 0.1216 95.3 1136.5739 1136.2963 4796.7 5 4.348 65.0 1 K.GAFQKVAATSR.- YDR385W 3 3 7.0% 842 93289 6.3 U EFT2 SGDID:S000002793, Chr IV from 1243220-1245748, Verified ORF, "Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin" YOR133W 3 3 7.0% 842 93289 6.3 U EFT1 SGDID:S000005659, Chr XV from 575098-577626, Verified ORF, "Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin" Jamie_alpha_091208_39.13870.13870.2 2.8399 0.231 100.0 2745.4373 2745.1543 9869.5 1 5.233 35.4 1 R.FVEPIDDCPAGNIIGLVGIDQFLLK.T Jamie_alpha_091208_7.03044.03044.2 2.7915 0.2505 100.0 1973.9521 1973.2365 7372.8 95 4.819 35.3 1 R.GQVVSEEQRPGTPLFTVK.A Jamie_alpha_091208_19.07181.07181.2 2.7214 0.29 100.0 1799.8866 1801.0087 7844.8 8 5.54 43.3 1 K.AYLPVNESFGFTGELR.Q YOL086C 1 1 6.9% 348 36849 6.7 U ADH1 SGDID:S000005446, Chr XV from 160593-159547, reverse complement, Verified ORF, "Alcohol dehydrogenase, fermentative isozyme active as homo- or heterotetramers; required for the reduction of acetaldehyde to ethanol, the last step in the glycolytic pathway" * Jamie_alpha_091208_11.04378.04378.2 2.8338 0.2793 100.0 2312.1826 2313.4863 6498.2 4 4.93 28.3 1 K.ATDGGAHGVINVSVSEAAIEASTR.Y YPL229W 1 1 6.8% 206 23260 6.3 U YPL229W SGDID:S000006150, Chr XVI from 117067-117687, Uncharacterized ORF, "Hypothetical protein" * Jamie_alpha_091208_7.02851.02851.1 1.3139 0.2507 100.0 1517.73 1516.6049 6725.8 2 4.472 38.5 1 Q.PISPPLFLVGAGT*S*.E Reverse_YNL053W 1 1 6.7% 489 54218 7.2 U MSG5 SGDID:S000004998, Chr XIV from 529943-531412, Verified ORF, "Dual-specificity protein phosphatase required for maintenance of a low level of signaling through the cell integrity pathway; regulates and is regulated by Slt2p; also required for adaptive response to pheromone" * Jamie_alpha_091208_37.13254.13254.3 3.1887 0.1507 93.1 3896.7244 3896.349 7834.8 199 3.976 18.0 1 R.MIYAVILSASRS*VGCQCHVLIKKGQS*HATHIIR.T YDR372C 1 1 6.7% 345 39287 5.1 U VPS74 SGDID:S000002780, Chr IV from 1222139-1221102, reverse complement, Verified ORF, "Non-essential protein of unknown function involved in vacuolar protein sorting; belongs to a family of cytosolic Golgi-associated proteins suggesting that it may play a role in secretion; also detected in the nucleus" * Jamie_alpha_091208_52.18420.18420.2 2.725 0.0703 95.6 2661.7122 2658.9678 9083.3 72 3.482 27.3 1 K.NDEPLSISNWIDLLSGETWNLLK.I YAL025C 1 1 6.5% 306 35694 5.3 U MAK16 SGDID:S000000023, Chr I from 101146-100226, reverse complement, Verified ORF, "Essential nuclear protein, constituent of 66S pre-ribosomal particles; required for normal concentration of free 60S ribosomal subunits; required for maintenance of M1 satellite double-stranded RNA of the L-A virus" * Jamie_alpha_091208_12.04735.04735.2 3.1056 0.1068 98.2 2403.0642 2403.4749 8362.5 35 3.395 34.2 1 K.VEIEYEEEHEVQNAEQEVAQ.- YPL124W 2 3 6.3% 253 29280 9.5 U SPC29 SGDID:S000006045, Chr XVI from 316754-317515, Verified ORF, "Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inner plaque component Spc110p; required for SPB duplication" * Jamie_alpha_091208_7.02960.02960.2 4.4747 0.3115 100.0 1925.8889 1923.9855 5575.3 1 6.154 66.7 2 R.SSQIHIENEST*EDILK.I * Jamie_alpha_091208_7.03051.03051.3 3.6636 0.1399 97.8 1926.6112 1923.9855 7305.8 2 4.597 43.3 1 R.SSQIHIENES*TEDILK.I Reverse_YNL263C 1 1 6.1% 314 35498 8.9 U YIF1 SGDID:S000005207, Chr XIV from 147841-146897, reverse complement, Verified ORF, "Integral membrane protein required for the fusion of ER-derived COPII transport vesicles with the Golgi; interacts with Yip1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles" * Jamie_alpha_091208_8.03404.03404.2 2.1147 0.1408 95.2 2205.4722 2205.2139 8245.3 38 3.821 30.6 1 K.KVTS*KSIS*VHIDDDEAGSR.S YNL225C 2 3 6.0% 581 67400 6.0 U CNM67 SGDID:S000005169, Chr XIV from 224470-222725, reverse complement, Verified ORF, "Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration" * Jamie_alpha_091208_1.00133.00133.2 2.3475 0.0463 93.7 1279.6218 1277.3335 5864.7 88 3.723 60.0 2 K.DKS*PIGTDVHK.K * Jamie_alpha_091208_49.17301.17301.2 3.0736 0.263 100.0 2776.2798 2778.0845 10242.4 8 4.821 32.6 1 K.AELFEIPIGILFFDLYDSEENSSK.L YGL253W 1 1 6.0% 486 53943 5.3 U HXK2 SGDID:S000003222, Chr VII from 23935-25395, Verified ORF, "Hexokinase isoenzyme 2 that catalyzes phosphorylation of glucose in the cytosol; predominant hexokinase during growth on glucose; functions in the nucleus to repress expression of HXK1 and GLK1 and to induce expression of its own gene" * Jamie_alpha_091208_32.11775.11775.2 2.6048 0.1514 98.2 3459.0122 3459.6147 9371.6 1 5.158 25.0 1 R.IEEDPFENLEDTDDLFQNEFGINTTVQER.K YOR221C 1 1 5.8% 360 40707 6.9 U MCT1 SGDID:S000005747, Chr XV from 757558-756476, reverse complement, Verified ORF, "Predicted malonyl-CoA:ACP transferase, putative component of a type-II mitochondrial fatty acid synthase that produces intermediates for phospholipid remodeling" * Jamie_alpha_091208_10.04009.04009.2 2.2785 0.2597 91.7 2449.1802 2447.8115 6864.4 81 4.381 27.5 1 K.FEMWALSSPRATDLPQEVQKL.L YJR123W 1 1 5.8% 225 25039 8.6 U RPS5 SGDID:S000003884, Chr X from 651816-652493, Verified ORF, "Protein component of the small (40S) ribosomal subunit, the least basic of the non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; has similarity to E. coli S7 and rat S5 ribosomal proteins" * Jamie_alpha_091208_15.05912.05912.2 2.2832 0.1305 96.7 1340.8119 1340.609 6881.2 1 4.147 62.5 1 R.VNQAIALLTIGAR.E Reverse_YOR334W 1 1 5.7% 470 54203 9.4 U MRS2 SGDID:S000005861, Chr XV from 944591-946003, Verified ORF, "Mitochondrial inner membrane Mg(2+) channel, required for maintenance of intramitochondrial Mg(2+) concentrations at the correct level to support splicing of group II introns" * Jamie_alpha_091208_75.26135.26135.3 2.8499 0.1484 90.8 3192.1743 3195.439 6625.0 84 3.57 23.1 1 K.S*ELDY*MLVSLKAAASPNTTDFVYVKDR.E YCL028W 1 1 5.7% 405 42580 6.6 U RNQ1 SGDID:S000000533, Chr III from 70150-71367, Verified ORF, "[PIN(+)] prion, an infectious protein conformation that is generally an ordered protein aggregate" * Jamie_alpha_091208_17.06363.06363.2 3.6771 0.409 100.0 2506.2122 2506.7441 4478.2 1 5.567 36.4 1 K.LTSAAQSNPNDEQMSTIESLIQK.I YDR074W 1 1 5.6% 896 102976 8.1 U TPS2 SGDID:S000002481, Chr IV from 593889-596579, Verified ORF, "Phosphatase subunit of the trehalose-6-phosphate synthase/phosphatase complex, which synthesizes the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway" * Jamie_alpha_091208_54.18990.18990.4 2.9192 0.2087 100.0 5374.9717 5371.9683 8065.5 32 4.129 14.3 1 K.KTVAKAHLTDPQQVLETLGLLVGDVSLFQSAGTVDLDSRGHVKNS*ESSLK.S YKL054C 1 1 5.6% 738 83973 5.0 U DEF1 SGDID:S000001537, Chr XI from 338397-336181, reverse complement, Verified ORF, "RNAPII degradation factor, forms a complex with Rad26p in chromatin, enables ubiquitination and proteolysis of RNAPII" * Jamie_alpha_091208_7.02750.02750.4 5.2871 0.204 100.0 4705.0273 4705.733 8898.0 284 3.557 15.8 1 K.VNEQETSAQEQEEETAEPSEENEDRVPEVDGEEVQEEAEKK.E YHR019C 1 1 5.6% 554 62207 5.8 U DED81 SGDID:S000001061, Chr VIII from 143551-141887, reverse complement, Verified ORF, "Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA" * Jamie_alpha_091208_21.08016.08016.4 3.5616 0.1385 92.9 3579.644 3579.7717 9043.8 45 3.772 19.4 1 K.DAITWLNEHDIKNEEGEDFKFGDDIAEAAER.K YBL057C 1 1 5.6% 214 23128 5.4 U PTH2 SGDID:S000000153, Chr II from 113447-112803, reverse complement, Verified ORF, "One of two (see also PTH1) mitochondrially-localized peptidyl-tRNA hydrolases; dispensable for cell growth" * Jamie_alpha_091208_9.03659.03659.2 2.6364 0.088 96.7 1432.7855 1431.6042 8091.2 2 4.021 59.1 1 K.SSAT*LLRSKEMK.E YOL092W 1 1 5.5% 308 34873 4.9 U YOL092W SGDID:S000005452, Chr XV from 144203-145129, Uncharacterized ORF, "Hypothetical protein" * Jamie_alpha_091208_7.02993.02993.2 3.0437 0.2187 100.0 1722.8322 1720.765 7355.3 1 4.573 43.8 1 E.EMAAPSSDGNAGDDNLR.E Reverse_YLR167W 1 1 5.3% 152 17216 9.9 U RPS31 SGDID:S000004157, Chr XII from 498949-499407, Verified ORF, "Fusion protein that is cleaved to yield a ribosomal protein of the small (40S) subunit and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B" * Jamie_alpha_091208_2.00435.00435.2 2.1174 0.0485 91.7 871.5125 872.0615 4414.9 80 3.103 71.4 1 R.KKGGGRLR.L YKL042W 2 2 5.2% 363 42271 7.9 U SPC42 SGDID:S000001525, Chr XI from 358119-359210, Verified ORF, "Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane" * Jamie_alpha_091208_9.03726.03726.2 4.3355 0.3879 100.0 2333.8613 2334.2546 8847.7 1 5.959 52.8 1 R.DY*SPSSDACLECSNDLYEK.N * Jamie_alpha_091208_7.02856.02856.2 3.7032 0.3997 100.0 2334.8723 2334.2546 6721.0 1 6.384 41.7 1 R.DYS*PSSDACLECSNDLYEK.N YLR153C 2 2 5.1% 683 75492 6.7 U ACS2 SGDID:S000004143, Chr XII from 447576-445525, reverse complement, Verified ORF, "Acetyl-coA synthetase isoform, required for growth on glucose; expressed under anaerobic conditions" * Jamie_alpha_091208_6.02670.02670.2 2.5108 0.2202 100.0 1639.8522 1639.8027 6209.1 2 4.472 50.0 1 R.VGEAEIAKYDTSSLR.V * Jamie_alpha_091208_21.07836.07836.2 2.4139 0.1622 98.2 2342.141 2342.6128 7793.6 21 3.998 31.6 1 R.VLGSVGEPISPDLWEWYHEK.V Reverse_YOR324C 1 1 5.0% 602 67293 9.4 U FRT1 SGDID:S000005851, Chr XV from 925035-923227, reverse complement, Verified ORF, "Tail-anchored endoplasmic reticulum membrane protein that is a substrate of the phosphatase calcineurin, interacts with homolog Frt2p, promotes cell growth in conditions of high Na+, alkaline pH, and cell wall stress" * Jamie_alpha_091208_62.21513.21513.2 1.7213 0.1326 93.0 3499.5923 3501.584 7635.5 7 4.537 20.7 1 R.NSNFDT*KPS*VRFPDDLSSAGSYSRSHRGVR.N YBR118W 1 1 5.0% 458 50033 9.0 U TEF2 SGDID:S000000322, Chr II from 477665-479041, Verified ORF, "Translational elongation factor EF-1 alpha; also encoded by TEF1; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA) to ribosomes" YPR080W 1 1 5.0% 458 50033 9.0 U TEF1 SGDID:S000006284, Chr XVI from 700592-701968, Verified ORF, "Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA) to ribosomes" Jamie_alpha_091208_7.02922.02922.2 2.3804 0.065 93.7 2551.3123 2551.793 6076.8 7 3.8 31.8 1 K.SVEMHHEQLEQGVPGDNVGFNVK.N YML105C 1 1 4.8% 273 31170 9.1 U SEC65 SGDID:S000004573, Chr XIII from 58687-57866, reverse complement, Verified ORF, "Subunit of the signal recognition particle (SRP), involved in protein targeting to the ER; interacts with Srp54p; homolog of mammalian SRP19" * Jamie_alpha_091208_6.02602.02602.2 2.8154 0.1403 99.0 1456.7517 1457.5834 6613.5 160 4.207 54.2 1 K.ENGQLIGAAT*KFK.G YBR031W 1 1 4.7% 362 39092 10.6 U RPL4A SGDID:S000000235, Chr II from 300166-301254, Verified ORF, "N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to Rpl4Bp and has similarity to E. coli L4 and rat L4 ribosomal proteins" YDR012W 1 1 4.7% 362 39062 10.6 U RPL4B SGDID:S000002419, Chr IV from 471850-472938, Verified ORF, "Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl4Ap and has similarity to E. coli L4 and rat L4 ribosomal proteins" Jamie_alpha_091208_10.03886.03886.3 3.1934 0.3587 100.0 1846.9777 1847.0782 6041.2 1 5.378 39.1 1 K.VGYTLPSHIISTSDVTR.I YBR079C 1 1 4.6% 964 110344 6.3 U RPG1 SGDID:S000000283, Chr II from 398271-395377, reverse complement, Verified ORF, "Subunit of the core complex of translation initiation factor 3(eIF3), essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes" * Jamie_alpha_091208_10.03976.03976.4 4.4553 0.1072 96.3 5061.217 5062.149 6478.6 2 4.34 17.1 1 K.EVSEEENTEPEVQEEKEETDEALGPQETEDGEEKEEESDPVIIR.N YGR124W 1 1 4.5% 572 64593 5.9 U ASN2 SGDID:S000003356, Chr VII from 739949-741667, Verified ORF, "Asparagine synthetase, isozyme of Asn1p; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway" * Jamie_alpha_091208_53.18591.18591.2 2.122 0.1285 95.4 3271.6743 3271.6165 10419.6 49 3.388 24.0 1 K.ITRYFTPDWLDEKRIPST*PVDYHAIR.H YLR019W 1 1 4.5% 397 44772 4.8 U PSR2 SGDID:S000004009, Chr XII from 180287-181480, Verified ORF, "Functionally redundant Psr1p homolog, a plasma membrane phosphatase involved in the general stress response; required with Psr1p and Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p" * Jamie_alpha_091208_53.18568.18568.2 2.0492 0.2237 98.9 2036.9829 2035.0667 7898.2 206 4.067 29.4 1 K.T*MQENGNSGNGKLAPLS*R.D YOL038W 1 1 4.3% 254 28439 7.4 U PRE6 SGDID:S000005398, Chr XV from 255335-256099, Verified ORF, "20S proteasome alpha-type subunit" * Jamie_alpha_091208_9.03577.03577.2 2.2471 0.2495 100.0 1144.0013 1145.3416 4548.1 90 4.722 65.0 1 R.SLLEVVQTGAK.N YJL138C 1 1 4.1% 395 44697 5.1 U TIF2 SGDID:S000003674, Chr X from 154609-153422, reverse complement, Verified ORF, "Translation initiation factor eIF4A, identical to Tif1p; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G" YKR059W 1 1 4.1% 395 44697 5.1 U TIF1 SGDID:S000001767, Chr XI from 554629-555816, Verified ORF, "Translation initiation factor eIF4A, identical to Tif2p; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G" Jamie_alpha_091208_14.05410.05410.2 4.1034 0.3235 100.0 1786.6522 1785.9536 7427.8 1 7.074 56.7 1 R.GVFGYGFEEPSAIQQR.A YGR279C 1 1 4.1% 386 40173 4.8 U SCW4 SGDID:S000003511, Chr VII from 1049964-1048804, reverse complement, Verified ORF, "Cell wall protein with similarity to glucanases; scw4 scw10 double mutants exhibit defects in mating" * Jamie_alpha_091208_8.03412.03412.2 2.3115 0.1506 98.3 1885.899 1887.0656 5080.5 37 4.37 40.0 1 R.LYGTDCNQVENVFKAK.A Reverse_YIL077C 1 1 4.1% 320 36946 8.9 U YIL077C SGDID:S000001339, Chr IX from 215950-214988, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_alpha_091208_23.08457.08457.2 1.9637 0.1178 94.2 1466.7781 1464.6272 7295.2 11 3.296 54.2 1 -.SRNGGYRGSQLLR.Q YJL053W 1 1 4.0% 379 42547 8.3 U PEP8 SGDID:S000003589, Chr X from 335814-336953, Verified ORF, "Vacuolar protein sorting protein that forms part of the multimeric membrane-associated retromer complex along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport" * Jamie_alpha_091208_22.08434.08434.4 2.807 0.1504 93.5 1777.4565 1773.857 3315.6 13 3.822 34.5 1 R.KHVDIATRSS*NSSYK.S YBR193C 1 1 4.0% 223 25268 5.5 U MED8 SGDID:S000000397, Chr II from 609748-609077, reverse complement, Verified ORF, "Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation" * Jamie_alpha_091208_14.05399.05399.2 1.9369 0.0998 94.2 1041.5122 1039.2229 5847.6 3 3.568 68.8 1 R.LAQLTHSLR.R YKL012W 1 1 3.9% 583 69065 8.9 U PRP40 SGDID:S000001495, Chr XI from 417953-419704, Verified ORF, "U1 snRNP protein involved in splicing, interacts with the branchpoint-binding protein during the formation of the second commitment complex" * Jamie_alpha_091208_62.21777.21777.3 3.216 0.2077 100.0 2917.75 2914.0715 8446.9 76 4.018 27.3 1 K.EAKDASGRIY*YYNTLTKKST*WEK.P Reverse_YPR055W 1 1 3.8% 1065 122224 6.8 U SEC8 SGDID:S000006259, Chr XVI from 667673-670870, Verified ORF, "Essential 121kDa subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, and Exo84p), which has the essential function of mediating polarized targeting of secretory vesicles to active sites of exocytosis" * Jamie_alpha_091208_17.06558.06558.3 3.2305 0.2084 97.8 4624.995 4622.1294 9152.1 297 3.632 15.6 1 K.NINNITKLLFGIRNFGENEQNRSPALPAQT*AANEQLMINGK.N YLR303W 1 1 3.8% 444 48672 6.4 U MET17 SGDID:S000004294, Chr XII from 732544-733878, Verified ORF, "O-acetyl homoserine-O-acetyl serine sulfhydrylase, required for sulfur amino acid synthesis" * Jamie_alpha_091208_15.05725.05725.2 2.5699 0.0942 95.4 2087.2476 2087.2065 8712.6 26 3.397 37.5 1 R.FVEGDNPEEFEKVFDER.T Reverse_YHR097C 1 1 3.8% 366 40663 9.9 U YHR097C SGDID:S000001139, Chr VIII from 298612-298488,298363-297388, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_alpha_091208_45.16035.16035.2 1.9489 0.0973 91.2 1609.017 1611.8821 7845.2 11 3.446 46.2 1 K.DITDVNKPPKVKTR.S YOL094C 1 1 3.7% 323 36149 9.1 U RFC4 SGDID:S000005454, Chr XV from 142554-141583, reverse complement, Verified ORF, "Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon" * Jamie_alpha_091208_9.03729.03729.2 2.2298 0.0501 90.3 1345.6997 1346.656 5908.5 4 3.065 63.6 1 K.IVDSPHPLIVKK.M contaminant_KERATIN22 1 1 3.6% 645 65865 8.0 U no description * Jamie_alpha_091208_2.00740.00740.2 2.3097 0.2436 100.0 1741.7722 1742.716 7025.4 1 4.869 38.6 1 R.GGSGGGGSISGGGYGSGGGSGGR.Y YMR273C 1 1 3.5% 915 103358 6.4 U ZDS1 SGDID:S000004886, Chr XIII from 813979-811232, reverse complement, Verified ORF, "Protein that interacts with silencing proteins at the telomere, involved in transcriptional silencing; has a role in localization of Bcy1p, a regulatory subunit of protein kinase A; implicated in mRNA nuclear export" * Jamie_alpha_091208_53.18616.18616.3 3.4632 0.2917 100.0 3458.0044 3455.6016 11808.0 127 4.532 20.2 1 K.PHHKHDASSSPSSSPS*SSPSIPNNDAVHVRVR.K YJL158C 1 1 3.5% 227 23242 4.7 U CIS3 SGDID:S000003694, Chr X from 122865-122182, reverse complement, Verified ORF, "Mannose-containing glycoprotein constituent of the cell wall; member of the PIR (proteins with internal repeats) family" YKL164C 1 1 2.3% 341 34622 6.5 U PIR1 SGDID:S000001647, Chr XI from 142824-141799, reverse complement, Verified ORF, "O-glycosylated protein required for cell wall stability; attached to the cell wall via beta-1,3-glucan; mediates mitochondrial translocation of Apn1p; expression regulated by the cell integrity pathway and by Swi5p during the cell cycle" YKL163W 1 1 2.5% 325 33004 5.7 U PIR3 SGDID:S000001646, Chr XI from 144406-145383, Verified ORF, "O-glycosylated covalently-bound cell wall protein required for cell wall stability; expression is cell cycle regulated, peaking in M/G1 and also subject to regulation by the cell integrity pathway" YJL160C 1 1 2.8% 287 30215 9.0 U YJL160C SGDID:S000003696, Chr X from 118820-117957, reverse complement, Uncharacterized ORF, "Hypothetical protein" YJL159W 1 1 2.1% 387 38619 5.4 U HSP150 SGDID:S000003695, Chr X from 120444-121607, Verified ORF, "O-mannosylated heat shock protein that is secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation" Jamie_alpha_091208_3.01110.01110.2 2.1514 0.1942 98.9 829.49133 829.9749 3629.1 1 4.384 85.7 1 R.IGSIVANR.Q Reverse_YOR322C 1 1 3.4% 818 89826 8.6 U LDB19 SGDID:S000005849, Chr XV from 921057-918601, reverse complement, Verified ORF, "Protein of unknown function; null mutant shows a reduced affinity for the alcian blue dye suggesting a decreased net negative charge of the cell surface" * Jamie_alpha_091208_15.05923.05923.4 3.4875 0.1496 96.2 2936.5923 2931.961 7519.6 183 3.656 22.8 1 R.AAGTPVSVINS*NSAGNSAKEKNT*SHNSK.N YCR053W 1 1 3.3% 514 57474 5.6 U THR4 SGDID:S000000649, Chr III from 216692-218236, Verified ORF, "Threonine synthase, conserved protein that catalyzes formation of threonine from 0-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway" * Jamie_alpha_091208_52.18363.18363.2 2.6158 0.0951 96.0 2059.4417 2060.3599 8562.4 304 3.898 34.4 1 K.DVALQFVGNLFEYFLQR.T YFL037W 1 1 3.3% 457 50923 4.7 U TUB2 SGDID:S000001857, Chr VI from 56335-57708, Verified ORF, "Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules" * Jamie_alpha_091208_12.04576.04576.2 2.6893 0.1525 98.1 1599.847 1599.7814 4400.4 3 3.495 60.7 1 R.SINVDLEPGTIDAVR.N YML076C 1 1 3.2% 944 107560 7.6 U WAR1 SGDID:S000004541, Chr XIII from 115347-112513, reverse complement, Verified ORF, "Homodimeric Zn2Cys6 zinc finger transcription factor; binds to a weak acid response element to induce transcription of PDR12 and FUN34, encoding an acid transporter and a putative ammonia transporter, respectively" * Jamie_alpha_091208_47.16776.16776.4 4.1631 0.2467 100.0 3332.7366 3334.3582 7102.6 45 4.282 20.7 1 K.GPT*DSEESSLKDGTSYLAS*FPSDPNAKQFP.N Reverse_YOR076C 1 1 3.2% 747 84779 7.8 U SKI7 SGDID:S000005602, Chr XV from 471621-469378, reverse complement, Verified ORF, "Antiviral adaptor protein that mediates interactions via its N-terminus between the exosome and Ski complex (Ski2p, Ski3p, Ski8p) which operate in the 3'-to-5' mRNA-decay pathway; cytoplasmic protein required for degrading nonstop mRNAs" * Jamie_alpha_091208_62.21680.21680.3 3.1471 0.1646 96.4 2765.6042 2765.1423 5710.5 131 3.959 26.1 1 K.KLASLKLSLSVSHQNPTFDS*RKVK.D Reverse_YNL293W 1 1 3.2% 633 72999 7.6 U MSB3 SGDID:S000005237, Chr XIV from 80640-82541, Verified ORF, "GTPase-activating protein for Sec4p and several other Rab GTPases, regulates exocytosis via its action on Sec4p, also required for proper actin organization; similar to Msb4p; both Msb3p and Msb4p localize to sites of polarized growth" * Jamie_alpha_091208_54.19052.19052.2 2.418 0.2094 98.9 2404.0024 2406.7654 7860.7 20 4.332 31.6 1 R.MQKVKEKISNNWHVGALS*KK.F YJR059W 1 1 3.1% 818 91400 8.9 U PTK2 SGDID:S000003820, Chr X from 545701-548157, Verified ORF, "Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake" * Jamie_alpha_091208_52.18176.18176.2 2.7177 0.3127 100.0 2679.2996 2677.8906 10387.5 6 5.343 31.2 1 N.NPYLNSPSDILGTGTGIASTRDRDR.A Reverse_YLR023C 1 1 3.1% 543 62571 9.2 U IZH3 SGDID:S000004013, Chr XII from 187128-185497, reverse complement, Verified ORF, "Membrane protein involved in zinc metabolism, member of the four-protein IZH family, expression induced by zinc deficiency; deletion reduces sensitivity to elevated zinc and shortens lag phase, overexpression reduces Zap1p activity" * Jamie_alpha_091208_56.19668.19668.3 2.8757 0.1452 91.5 2389.1274 2387.4495 8500.3 421 3.697 32.8 1 K.SHT*NYFRY*GHIIYRNER.W Reverse_YDR306C 1 1 3.1% 478 54437 8.6 U YDR306C SGDID:S000002714, Chr IV from 1075165-1073729, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_alpha_091208_72.24826.24826.2 2.6712 0.0965 95.9 1762.1548 1759.7876 4810.7 2 3.317 50.0 1 K.SALGLSGS*CELYLT*K.L YPL217C 1 1 3.0% 1183 135571 6.8 U BMS1 SGDID:S000006138, Chr XVI from 143170-139619, reverse complement, Verified ORF, "Essential conserved nucleolar GTP-binding protein required for synthesis of 40S ribosomal subunits and for processing of the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, has similarity to Tsr1p" * Jamie_alpha_091208_10.04074.04074.3 3.635 0.152 98.1 4092.8594 4090.0945 6770.5 51 3.613 19.1 1 R.IYGKPVQEEDADIDNLPSDEEPY*TNDDDVQDSEPR.M YDR395W 1 1 3.0% 944 108405 4.7 U SXM1 SGDID:S000002803, Chr IV from 1263314-1266148, Verified ORF, "Nuclear transport factor (karyopherin) involved in protein transport between the cytoplasm and nucleoplasm; similar to Nmd5p, Cse1p, Lph2p, and the human cellular apoptosis susceptibility protein, CAS1" * Jamie_alpha_091208_60.21141.21141.2 2.7707 0.2486 100.0 3148.0693 3145.4468 7134.1 1 4.611 29.6 1 R.DYIDSVIEELFPIVEGIASNIGSQTDYR.S YKR010C 1 1 2.9% 771 86366 8.0 U TOF2 SGDID:S000001718, Chr XI from 460882-458567, reverse complement, Verified ORF, "Nonessential mitochondrial protein of unknown function with sequence similarity to Net1p; identified as a topoisomerase I (Top1p) binding protein; displays synthetic genetic interactions with TOP1 and HPR1" * Jamie_alpha_091208_13.05093.05093.4 3.167 0.1439 93.8 2737.2874 2732.9236 6935.5 28 3.423 27.8 1 R.FKPT*GETKVQKRNSITEPY*YGK.F YBL099W 1 1 2.9% 545 58618 9.0 U ATP1 SGDID:S000000195, Chr II from 37050-38687, Verified ORF, "Alpha subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis" * Jamie_alpha_091208_15.05953.05953.2 2.792 0.1651 98.9 1566.7723 1564.8253 7553.9 12 3.918 43.3 1 R.TGNIVDVPVGPGLLGR.V YGR162W 1 1 2.8% 952 107101 6.0 U TIF4631 SGDID:S000003394, Chr VII from 824064-826922, Verified ORF, "Translation initiation factor eIF4G, subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); associates with the poly(A)-binding protein Pab1p, also interacts with eIF4A (Tif1p); homologous to Tif4632p" * Jamie_alpha_091208_58.20332.20332.3 3.8607 0.2099 100.0 2847.5225 2848.2231 9437.7 1 4.768 26.9 1 R.TGQATLEGSQLLDSLFGILDNIIQTAK.I YGL008C 1 1 2.8% 918 99619 5.1 U PMA1 SGDID:S000002976, Chr VII from 482671-479915, reverse complement, Verified ORF, "Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plasma membrane potential; part of the P2 subgroup of cation-transporting ATPases" YPL036W 1 1 2.7% 947 102172 5.0 U PMA2 SGDID:S000005957, Chr XVI from 482841-485684, Verified ORF, "Plasma membrane H+-ATPase, isoform of Pma1p, involved in pumping protons out of the cell; regulator of cytoplasmic pH and plasma membrane potential" Jamie_alpha_091208_9.03442.03442.3 4.147 0.2744 100.0 3022.4307 3022.2114 8160.6 1 4.863 28.0 1 K.TVEEDHPIPEDVHENYENKVAELASR.G YDL206W 1 1 2.8% 762 85983 6.2 U YDL206W SGDID:S000002365, Chr IV from 90177-92465, Uncharacterized ORF, "Hypothetical protein" * Jamie_alpha_091208_25.09472.09472.2 2.7102 0.3327 100.0 2405.112 2406.5254 6057.3 66 5.089 27.5 1 Y.VRPLGDTQIDEDNAISLDPT*R.L Reverse_YDR365C 1 1 2.7% 628 72410 5.3 U ESF1 SGDID:S000002773, Chr IV from 1206373-1204487, reverse complement, Verified ORF, "Nucleolar protein involved in pre-rRNA processing; depletion causes severely decreased 18S rRNA levels" * Jamie_alpha_091208_14.05363.05363.2 1.9083 0.1223 93.6 2006.0322 2004.1328 7574.5 41 3.266 37.5 1 K.GGKPVFS*SFTIMLDES*K.V YBL054W 1 1 2.7% 525 59226 9.3 U YBL054W SGDID:S000000150, Chr II from 117592-119169, Uncharacterized ORF, "Hypothetical protein" * Jamie_alpha_091208_23.08553.08553.2 2.2104 0.1152 95.5 1781.195 1779.9255 8988.5 191 4.062 42.3 1 R.RAS*LVVS*PYMSPRR.L contaminant_SPA1_STAAU 1 1 2.7% 524 57320 5.7 U owl|P02976| IMMUNOGLOBULIN G BINDING PROTEIN A PRECURSOR (PROTEIN A). - STAPHYLOCOCCUS... contaminant_SPA2_STAAU 1 1 2.8% 508 55439 5.7 U owl|P38507| IMMUNOGLOBULIN G BINDING PROTEIN A PRECURSOR (PROTEIN A). - STAPHYLOCOCCUS... Jamie_alpha_091208_6.02648.02648.2 3.6525 0.3244 100.0 1460.7109 1459.5529 5443.9 1 6.323 80.8 1 K.DDPSQSANLLAEAK.K YMR205C 1 1 2.6% 959 104618 6.7 U PFK2 SGDID:S000004818, Chr XIII from 674765-671886, reverse complement, Verified ORF, "Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes" * Jamie_alpha_091208_16.05967.05967.2 1.7208 0.1268 91.2 2966.912 2967.1104 8586.0 15 3.635 27.1 1 -.MT*VT*TPFVNGTSYCTVTAYSVQSYK.A YCR084C 1 1 2.5% 713 78308 5.7 U TUP1 SGDID:S000000680, Chr III from 262448-260307, reverse complement, Verified ORF, "General repressor of transcription, forms complex with Cyc8p, involved in the establishment of repressive chromatin structure through interactions with histones H3 and H4, appears to enhance expression of some genes" * Jamie_alpha_091208_10.03851.03851.2 3.1524 0.2731 100.0 1997.9321 1998.1107 5566.9 1 5.782 44.1 1 K.DVENLNTSSSPSSDLYIR.S Reverse_YLR072W 2 2 2.5% 693 78212 6.2 U YLR072W SGDID:S000004062, Chr XII from 278863-280944, Uncharacterized ORF, "Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern" * Jamie_alpha_091208_11.04349.04349.2 2.7698 0.1484 98.1 1824.9885 1826.0135 3583.0 154 3.779 40.6 1 R.PSGFS*TVSPLPKLTDAK.E * Jamie_alpha_091208_11.04407.04407.2 3.0421 0.0894 97.4 1827.9934 1826.0135 3663.2 17 3.445 50.0 1 R.PSGFST*VSPLPKLTDAK.E Reverse_YGL003C 1 1 2.5% 566 62822 8.6 U CDH1 SGDID:S000002971, Chr VII from 494178-492478, reverse complement, Verified ORF, "Cell-cycle regulated activator of the anaphase-promoting complex/cyclosome (APC/C), which directs ubiquitination of mitotic cyclins resulting in exit from mitosis; targets the APC/C to specific substrates including CDC20, ASE1 and CIN8" * Jamie_alpha_091208_3.01047.01047.4 3.2718 0.1273 92.6 1794.9043 1794.882 6733.7 164 3.752 34.6 1 R.RGKRQQYT*LLS*AGR.V YMR307W 1 1 2.5% 559 59582 4.7 U GAS1 SGDID:S000004924, Chr XIII from 887002-888681, Verified ORF, "Beta-1.3-glucanosyltransferase, required for cell wall assembly; localizes to the cell surface via a glycosylphosphatidylinositol (GPI) anchor" * Jamie_alpha_091208_23.08753.08753.2 2.1364 0.1655 98.3 1703.5294 1701.8297 8495.9 251 4.144 42.3 1 R.DDPTWTVDLFNSYK.T YOR086C 1 1 2.4% 1186 133576 7.2 U TCB1 SGDID:S000005612, Chr XV from 486780-483220, reverse complement, Verified ORF, "Contains three calcium and lipid binding domains; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; C-terminal portion of Tcb1p, Tcb2p and Tcb3p interact" * Jamie_alpha_091208_58.20295.20295.2 2.5619 0.2132 100.0 3374.791 3373.781 11016.0 1 5.592 28.6 1 R.DTLNPVWDETLYVLLNSFTDPLTISVYDK.R YIL166C 1 1 2.4% 542 61898 8.9 U YIL166C SGDID:S000001428, Chr IX from 32566-30938, reverse complement, Uncharacterized ORF, "Hypothetical protein, member of the Dal5p subfamily of the major facilitator family" * Jamie_alpha_091208_15.05909.05909.1 1.2155 0.2726 100.0 1721.58 1721.8241 5923.8 6 4.042 33.3 1 M.AVATKFYY*IS*RNK.Y YBR148W 1 1 2.3% 609 70175 7.1 U YSW1 SGDID:S000000352, Chr II from 537870-539699, Verified ORF, "Protein expressed specifically in spores" * Jamie_alpha_091208_51.17876.17876.2 2.0952 0.074 90.1 1736.9521 1735.851 8135.7 269 3.466 38.5 1 R.NSIFKS*ANDVKEFR.N YHR102W 1 1 2.2% 1080 117061 8.0 U KIC1 SGDID:S000001144, Chr VIII from 316575-319817, Verified ORF, "Protein kinase of the PAK/Ste20 kinase family, required for cell integrity possibly through regulating 1,6-beta-glucan levels in the wall; physically interacts with Cdc31p (centrin), which is a component of the spindle pole body" * Jamie_alpha_091208_58.20435.20435.3 3.1371 0.1352 91.9 2905.3765 2902.1738 7929.3 68 3.471 23.9 1 R.YYGSYLKDTSLWIIMEHCAGGS*LR.S YDR356W 2 2 2.2% 944 111782 7.1 U SPC110 SGDID:S000002764, Chr IV from 1186098-1188932, Verified ORF, "Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner" * Jamie_alpha_091208_13.05185.05185.3 5.1941 0.4327 100.0 2431.3076 2429.7332 6756.9 4 7.696 37.5 1 K.TVKDQVLELENNSDVQSLKLR.S * Jamie_alpha_091208_10.03880.03880.2 4.1032 0.3129 100.0 1833.8922 1831.9744 7484.5 1 5.95 66.7 1 K.DQVLELENNSDVQSLK.L YOR373W 1 1 2.2% 851 94104 7.0 U NUD1 SGDID:S000005900, Chr XV from 1036830-1039385, Verified ORF, "Component of the spindle pole body outer plaque, required for exit from mitosis" * Jamie_alpha_091208_6.02640.02640.2 3.8206 0.2871 100.0 2289.9172 2290.2268 5359.0 1 6.046 47.2 1 K.IQEEENLANSDDT*PLDT*PK.F Reverse_YGR266W 1 1 2.1% 701 81191 6.9 U YGR266W SGDID:S000003498, Chr VII from 1022662-1024767, Verified ORF, "Protein of unknown function;predicted to contain a single transmembrane domain; localized to both the mitochondrion and the plasma membrane" * Jamie_alpha_091208_22.08222.08222.2 2.4508 0.26 90.9 1765.8121 1766.963 6402.4 73 4.403 39.3 1 R.GVTLFNETKMFSDNY.S YMR186W 1 1 2.0% 705 80900 4.8 U HSC82 SGDID:S000004798, Chr XIII from 632354-634471, Verified ORF, "Cytoplasmic chaperone of the Hsp90 family, redundant in function and nearly identical with Hsp82p, and together they are essential; expressed constitutively at 10-fold higher basal levels that HSP82 and induced 2-3 fold by heat shock" YPL240C 1 1 2.0% 709 81406 4.9 U HSP82 SGDID:S000006161, Chr XVI from 98625-96496, reverse complement, Verified ORF, "Cytoplasmic chaperone (Hsp90 family) required for pheromone signaling and negative regulation of Hsf1p; docks with the mitochondrial import receptor Tom70p for preprotein delivery; interacts with co-chaperones Cns1p, Cpr6p, Cpr7p, and Sti1p" Jamie_alpha_091208_6.02467.02467.2 3.2806 0.1893 100.0 1739.7612 1740.7748 5759.7 1 4.761 69.2 1 K.DFELEETDEEKAER.E YNL287W 1 1 1.9% 935 104831 5.1 U SEC21 SGDID:S000005231, Chr XIV from 91994-94801, Verified ORF, "Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo" * Jamie_alpha_091208_20.07656.07656.2 2.1598 0.089 93.6 2199.6653 2202.3396 7779.0 256 3.476 32.4 1 K.FESVQLETAKLIT*SFAT*R.N YDL126C 1 1 1.9% 835 91996 4.9 U CDC48 SGDID:S000002284, Chr IV from 238664-236157, reverse complement, Verified ORF, "ATPase in ER, nuclear membrane and cytosol with homology to mammalian p97; in a complex with Npl4p and Ufd1p participates in retrotranslocation of ubiquitinated proteins from the ER into the cytosol for degradation by the proteasome" * Jamie_alpha_091208_21.07929.07929.2 2.3321 0.19 99.0 1741.8823 1739.9689 6321.1 132 3.945 40.0 1 K.ATQGFSGADLLYIVQR.A YDR443C 1 1 1.7% 1420 160000 5.8 U SSN2 SGDID:S000002851, Chr IV from 1349928-1345666, reverse complement, Verified ORF, "Protein required for stable association of Srb10p-Srb11p kinase with RNA polymerase holoenzyme; subunit of the RNA polymerase II mediator complex; essential for transcriptional regulation" * Jamie_alpha_091208_52.18434.18434.2 1.8792 0.1126 92.4 2773.4207 2774.832 10352.2 33 3.027 26.1 1 K.VNNSVS*KTGSVDTLHNKEGT*LEQR.E YLR067C 1 1 1.7% 965 112646 9.3 U PET309 SGDID:S000004057, Chr XII from 270711-267814, reverse complement, Verified ORF, "Specific translational activator for the COX1 mRNA, also influences stability of intron-containing COX1 primary transcripts; located in the mitochondrial inner membrane" * Jamie_alpha_091208_12.04571.04571.2 3.3578 0.0598 96.7 1771.853 1770.9774 10416.8 132 3.172 46.7 1 R.IENIENLPSSTTPNIK.L YDR176W 1 1 1.7% 702 79282 5.1 U NGG1 SGDID:S000002583, Chr IV from 814447-816555, Verified ORF, "Transcriptional regulator involved in glucose repression of Gal4p-regulated genes; component of transcriptional adaptor and histone acetyltransferase complexes, the ADA complex, the SAGA complex, and the SLIK complex" * Jamie_alpha_091208_47.16772.16772.2 2.0436 0.0982 94.3 1546.1129 1545.5162 7725.9 51 3.244 50.0 1 K.Y*NVAS*YPTNDLK.D Reverse_YPL137C 1 1 1.6% 1276 140891 7.4 U YPL137C SGDID:S000006058, Chr XVI from 296646-292816, reverse complement, Uncharacterized ORF, "Glc7-interacting protein whose overexpression relocalizes Glc7p from the nucleus" * Jamie_alpha_091208_44.15804.15804.2 2.2045 0.0779 92.4 2064.652 2063.146 6414.2 99 3.866 34.2 1 K.GKGVS*SS*ATANIASSALRPK.S YGL086W 1 1 1.6% 749 87651 5.4 U MAD1 SGDID:S000003054, Chr VII from 347122-349371, Verified ORF, "Coiled-coil protein involved in the spindle-assembly checkpoint; phosphorylated by Mps1p upon checkpoint activation which leads to inhibition of the activity of the anaphase promoting complex; forms a complex with Mad2p" * Jamie_alpha_091208_10.03847.03847.2 2.1122 0.0982 94.9 1484.7128 1487.6067 7880.4 146 3.331 50.0 1 K.YLIADLNENT*LK.S YAR009C 1 1 1.4% 1196 136732 8.6 U YAR009C SGDID:S000000067, Chr I from 164188-160598, reverse complement, transposable_element_gene, "TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition" YML045W 1 1 1.0% 1755 198524 8.2 U YML045W SGDID:S000004508, Chr XIII from 184461-185765,185767-189729, transposable_element_gene, "TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition" YML039W 1 1 1.0% 1755 198571 8.1 U YML039W SGDID:S000004503, Chr XIII from 196628-197932,197934-201896, transposable_element_gene, "TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition" YLR227W-B 1 1 1.0% 1755 198404 7.9 U YLR227W-B SGDID:S000007376, Chr XII from 593440-594744,594746-598708, transposable_element_gene, "TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition" YLR157C-B 1 1 1.0% 1755 198544 8.1 U YLR157C-B SGDID:S000007374, Chr XII from 481602-480298,480296-476334, reverse complement, transposable_element_gene, "TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition" YLR035C-A 1 1 1.5% 1155 131617 8.7 U YLR035C-A SGDID:S000007225, Chr XII from 218908-215441, reverse complement, transposable_element_gene, "Ty ORF" YJR029W 1 1 1.0% 1755 198616 8.3 U YJR029W SGDID:S000003790, Chr X from 478258-479558,479560-483526, transposable_element_gene, "TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition" YJR027W 1 1 1.0% 1755 198651 8.0 U YJR027W SGDID:S000003788, Chr X from 472674-473975,473977-477942, transposable_element_gene, "TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition" YHR214C-B 1 1 0.9% 1793 202816 8.3 U YHR214C-B SGDID:S000003534, Chr VIII from 549346-547931,547929-543964, reverse complement, transposable_element_gene, "TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition" YGR161C-D 1 1 1.0% 1755 198558 8.5 U YGR161C-D SGDID:S000007368, Chr VII from 823020-821716,821714-817752, reverse complement, transposable_element_gene, "TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition" YGR038C-B 1 1 1.0% 1755 198520 8.2 U YGR038C-B SGDID:S000007408, Chr VII from 567471-566167,566165-562203, reverse complement, transposable_element_gene, "TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition" YGR027W-B 1 1 1.0% 1755 198656 8.2 U YGR027W-B SGDID:S000007406, Chr VII from 536061-537365,537367-541329, transposable_element_gene, "TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition" YER160C 1 1 1.0% 1755 198568 8.5 U YER160C SGDID:S000000962, Chr V from 498119-496818,496816-492851, reverse complement, transposable_element_gene, "TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition" YER138C 1 1 1.0% 1755 198561 8.1 U YER138C SGDID:S000000940, Chr V from 449020-447719,447717-443752, reverse complement, transposable_element_gene, "TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition" YDR365W-B 1 1 1.0% 1755 198658 8.0 U YDR365W-B SGDID:S000007401, Chr IV from 1206987-1208291,1208293-1212255, transposable_element_gene, "TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition" YDR316W-B 1 1 1.0% 1755 198526 8.2 U YDR316W-B SGDID:S000007399, Chr IV from 1096059-1097363,1097365-1101327, transposable_element_gene, "TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition" YDR261C-D 1 1 1.1% 1604 181660 7.5 U YDR261C-D SGDID:S000007395, Chr IV from 992343-991039,991037-987528, reverse complement, transposable_element_gene, "TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition" YDR210C-D 1 1 1.0% 1755 198838 8.4 U YDR210C-D SGDID:S000007410, Chr IV from 883922-882618,882616-878654, reverse complement, transposable_element_gene, "TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition" YDR098C-B 1 1 1.0% 1755 198594 8.4 U YDR098C-B SGDID:S000007391, Chr IV from 651121-649817,649815-645853, reverse complement, transposable_element_gene, "TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition" YBR012W-B 1 1 1.0% 1756 198868 8.5 U YBR012W-B SGDID:S000002155, Chr II from 259867-261171,261173-265138, transposable_element_gene, "TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition" YBL005W-B 1 1 1.0% 1755 198968 8.2 U YBL005W-B SGDID:S000002147, Chr II from 221333-222637,222639-226601, transposable_element_gene, "TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition" Jamie_alpha_091208_53.18606.18606.2 3.8069 0.3577 100.0 1981.3674 1982.3275 8908.1 2 6.451 46.9 1 R.REDSILDVFTTILAFIK.N YGL207W 1 1 1.4% 1035 118630 5.1 U SPT16 SGDID:S000003175, Chr VII from 98973-102080, Verified ORF, "Subunit of the heterodimeric FACT complex (Spt16p-Pob3p), facilitates RNA Polymerase II transcription elongation through nucleosomes by destabilizing and then reassembling nucleosome structure" * Jamie_alpha_091208_17.06352.06352.2 2.5287 0.2278 100.0 1686.6322 1684.9304 5404.1 45 4.576 46.4 1 K.ALADVVQQDKLIENK.T YGL120C 1 1 1.3% 767 87562 6.4 U PRP43 SGDID:S000003088, Chr VII from 283943-281640, reverse complement, Verified ORF, "RNA helicase in the DEAH-box family, involved in release of the lariat-intron from the spliceosome" * Jamie_alpha_091208_13.04952.04952.2 1.957 0.1168 94.9 1205.6322 1203.2511 5666.9 63 3.547 61.1 1 K.LGEEVGYS*IR.F Reverse_YPR018W 1 1 1.3% 606 70210 6.7 U RLF2 SGDID:S000006222, Chr XVI from 594473-596293, Verified ORF, "Largest subunit (p90) of the Chromatin Assembly Complex (CAF-I) with Cac2p and Msi1p that assembles newly synthesized histones onto recently replicated DNA; involved in the maintenance of transcriptionally silent chromatin" * Jamie_alpha_091208_1.00019.00019.2 2.0494 0.0528 90.5 1029.6504 1029.2308 5299.1 17 3.425 71.4 1 K.QKKLEARR.Q YDR150W 1 1 0.4% 2748 313032 5.4 U NUM1 SGDID:S000002557, Chr IV from 755623-763869, Verified ORF, "Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex" * Jamie_alpha_091208_60.21102.21102.2 1.8524 0.0945 90.1 1537.738 1536.5618 6251.1 2 3.023 54.5 1 K.RLSAS*RRSVST*R.S YKR054C 1 1 0.2% 4092 471351 6.3 U DYN1 SGDID:S000001762, Chr XI from 547567-535289, reverse complement, Verified ORF, "Cytoplasmic heavy chain dynein, microtubule motor protein, required for anaphase spindle elongation; involved in spindle assembly, chromosome movement, and spindle orientation during cell division, targeted to microtubule tips by Pac1p" * Jamie_alpha_091208_13.05070.05070.2 1.9446 0.1682 98.2 1109.5339 1108.2085 3660.2 3 4.102 75.0 1 K.ESLY*GSMLK.A Proteins Peptide IDs Spectra Unfiltered 10063 46767 51529 Filtered 135 275 291 Forward matches 112 251 267 Decoy matches 23 24 24 Forward FP rate 20.54 9.56 8.99 Classification Nonredundant Proteins Redundant Proteins Unclassified 0 0