DTASelect v2.0.16 /data/1/catclw/Projects/Jamie/IMAC_elute_b/combine_1_2 /garibaldi/people-b/applications/yates/dbase/SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta SEQUEST 3.0 in SQT format. -p 1 --fp 0.1 --modstat --sp --noxc --nodcn --extra -e con true Use criteria 0.0 Minimum peptide confidence 0.1 Peptide false positive rate 0.0 Minimum protein confidence 1.0 Protein false positive rate 1 Minimum charge state 16 Maximum charge state 0.0 Minimum ion proportion 1000 Maximum Sp rank -1.0 Minimum Sp score Include Modified peptide inclusion Any Tryptic status requirement false Multiple, ambiguous IDs allowed Ignore Peptide validation handling XCorr Purge duplicate peptides by protein false Include only loci with unique peptide true Remove subset proteins Ignore Locus validation handling con Exclude protein names matching 0 Minimum modified peptides per locus 1000 Minimum redundancy for low coverage loci 1 Minimum peptides per locus Locus Sequence Count Spectrum Count Sequence Coverage Length MolWt pI Validation Status Descriptive Name Unique FileName XCorr DeltCN Conf% M+H+ CalcM+H+ TotalIntensity SpR ZScore IonProportion Redundancy Sequence YBR109C 5 7 49.7% 147 16135 4.3 U CMD1 SGDID:S000000313, Chr II from 458356-457913, reverse complement, Verified ORF, "Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin" * Jamie_elute_b_02_itms_13.07445.07445.3 5.974 0.5341 100.0 4108.5244 4109.526 6002.6 1 9.146 29.2 3 R.SLGLSPSEAEVNDLMNEIDVDGNHQIEFSEFLALMSR.Q * Jamie_elute_b_01_itms_12.06904.06904.3 4.5739 0.1834 100.0 4115.4243 4113.3384 7737.7 1 5.222 20.7 1 R.SLGLSPSEAEVNDLMNEIDVDGNHQIEFS*EFLALMS*.R * Jamie_elute_b_01_itms_10.05564.05564.3 3.9082 0.1889 100.0 3368.3943 3368.651 8337.7 1 5.218 30.0 1 T.SIGEKLTDAEVDDMLREVSDGSGEINIQQFA.A * Jamie_elute_b_01_itms_12.06609.06609.3 4.7234 0.2637 100.0 3369.2944 3366.7217 9234.0 1 5.026 30.8 1 K.LTDAEVDDMLREVSDGSGEINIQQFAALLSK.- * Jamie_elute_b_02_itms_11.05891.05891.2 4.0796 0.3316 100.0 2106.5522 2107.325 8071.3 1 7.127 47.4 1 R.EVSDGSGEINIQQFAALLSK.- YOR257W 3 3 46.6% 161 18751 4.6 U CDC31 SGDID:S000005783, Chr XV from 811006-811491, Verified ORF, "Component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; interacts with Kar1p" * Jamie_elute_b_01_itms_10.05452.05452.3 3.9635 0.2396 100.0 2679.0842 2679.9866 9474.7 2 5.631 33.0 1 K.ALGFELPKREILDLIDEYDSEGR.H * Jamie_elute_b_01_itms_7.03720.03720.2 2.8326 0.2683 100.0 1382.1921 1380.5549 7698.3 1 5.7 75.0 1 K.YDDFYIVMGEK.I * Jamie_elute_b_02_itms_12.06805.06805.3 5.8388 0.502 100.0 4633.5244 4633.953 5290.9 1 8.87 25.0 1 R.VAKELGETLTDEELRAMIEEFDLDGDGEINENEFIAICTDS.- YIL002W-A 2 2 39.1% 69 7729 4.7 U YIL002W-A SGDID:S000028835, Chr IX from 350298-350507, Uncharacterized ORF, "Identified by expression profiling and mass spectrometry" * Jamie_elute_b_01_itms_12.06748.06748.2 3.649 0.364 100.0 2327.8123 2328.622 8391.6 1 6.514 40.5 1 R.DTPEDVSTAGAKDILDVLNLLK.G * Jamie_elute_b_01_itms_9.05019.05019.3 3.2609 0.2326 100.0 1912.3744 1913.2218 5282.2 59 5.539 35.3 1 A.GAKDILDVLNLLKGGEEK.I YKL042W 8 9 32.5% 363 42271 7.9 U SPC42 SGDID:S000001525, Chr XI from 358119-359210, Verified ORF, "Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane" * Jamie_elute_b_01_itms_7.03601.03601.2 3.1937 0.3754 100.0 2527.172 2528.7546 4774.0 1 6.651 47.4 1 R.LYDDYYNIPYQYSNPTPMNR.D * Jamie_elute_b_01_itms_6.03403.03403.3 4.0381 0.429 100.0 3433.9744 3435.6619 3991.9 1 7.632 29.6 2 R.LYDDYYNIPYQYSNPTPMNRDYNDVGSR.I * Jamie_elute_b_01_itms_4.01874.01874.3 3.5114 0.3321 100.0 1577.0044 1575.8052 7619.1 1 5.287 47.9 1 R.INADKLVPEEYKR.N * Jamie_elute_b_02_itms_7.03820.03820.2 3.2744 0.3937 100.0 1297.2522 1297.5554 7710.2 1 6.802 80.0 1 R.TLSVLTNYVMR.S * Jamie_elute_b_01_itms_9.05032.05032.2 2.116 0.2956 100.0 1376.3121 1377.5554 7565.1 1 5.278 75.0 1 R.TLS*VLTNYVMR.S * Jamie_elute_b_01_itms_9.05108.05108.3 5.5854 0.3368 100.0 3274.7043 3272.5837 4051.7 1 6.166 32.4 1 R.KLS*LNNQLQELQSMMDGDDNIKLDNVSK.H * Jamie_elute_b_02_itms_6.02942.02942.2 2.7114 0.4695 100.0 2332.7522 2334.2546 7334.4 1 6.808 50.0 1 R.DYS*PSSDACLECSNDLYEK.N * Jamie_elute_b_01_itms_3.01688.01688.2 4.522 0.4578 100.0 2148.9521 2148.355 7984.7 1 8.529 63.9 1 R.VKPENNMSETFATPTPNNR.- YBR010W 1 1 23.5% 136 15356 11.4 U HHT1 SGDID:S000000214, Chr II from 256329-256739, Verified ORF, "One of two identical histone H3 proteins (see also HHT2); core histone required for chromatin assembly, involved in heterochromatin-mediated telomeric and HM silencing; regulated by acetylation, methylation, and mitotic phosphorylation" YNL031C 1 1 23.5% 136 15356 11.4 U HHT2 SGDID:S000004976, Chr XIV from 576051-575641, reverse complement, Verified ORF, "One of two identical histone H3 proteins (see also HHT1); core histone required for chromatin assembly, involved in heterochromatin-mediated telomeric and HM silencing; regulated by acetylation, methylation, and mitotic phosphorylation" Jamie_elute_b_01_itms_14.07968.07968.3 7.0474 0.4995 100.0 3424.9143 3424.832 4626.9 1 8.282 33.1 1 R.FQSSAIGALQESVEAYLVSLFEDTNLAAIHAK.R YBL003C 1 1 22.0% 132 13989 10.7 U HTA2 SGDID:S000000099, Chr II from 235795-235397, reverse complement, Verified ORF, "One of two nearly identical (see also HTA1) histone H2A subtypes; core histone required for chromatin assembly and chromosome function; DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p " YDR225W 1 1 22.0% 132 13989 10.7 U HTA1 SGDID:S000002633, Chr IV from 915524-915922, Verified ORF, "One of two nearly identical (see also HTA2) histone H2A subtypes; core histone required for chromatin assembly and chromosome function; DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p " Jamie_elute_b_01_itms_17.09594.09594.3 3.8379 0.3149 100.0 2946.2644 2947.4014 7066.2 1 5.145 28.6 1 R.IGSGAPVYLTAVLEYLAAEILELAGNAAR.D YOL161C 1 1 19.2% 120 12877 5.9 U YOL161C SGDID:S000005521, Chr XV from 11910-11548, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_elute_b_01_itms_12.06674.06674.2 2.3316 0.2288 95.7 2057.3323 2059.4111 7438.3 12 5.328 29.5 1 M.VKLTSIAAGVAAIAAGASATTTL.A YNL225C 10 14 17.4% 581 67400 6.0 U CNM67 SGDID:S000005169, Chr XIV from 224470-222725, reverse complement, Verified ORF, "Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration" * Jamie_elute_b_01_itms_10.05741.05741.2 3.4319 0.4434 100.0 2419.5923 2420.6936 7005.8 1 7.326 47.5 1 K.SILPLFVNPEDVINCNFSNAR.D * Jamie_elute_b_01_itms_10.05719.05719.3 4.301 0.3351 100.0 2420.8145 2420.6936 9142.3 1 6.268 40.0 1 K.SILPLFVNPEDVINCNFSNAR.D * Jamie_elute_b_01_itms_4.02057.02057.2 3.0117 0.3223 100.0 1331.6721 1332.4967 6178.8 14 6.143 68.2 1 A.NSLQKELEAATK.S * Jamie_elute_b_01_itms_13.07244.07244.3 6.2908 0.5321 100.0 2905.8542 2906.2585 11752.6 1 9.374 38.5 1 R.KAELFEIPIGILFFDLYDSEENSSK.L * Jamie_elute_b_01_itms_13.07200.07200.2 4.981 0.3546 100.0 2908.0522 2906.2585 8137.4 1 6.398 45.8 2 R.KAELFEIPIGILFFDLYDSEENSSK.L * Jamie_elute_b_01_itms_12.06951.06951.3 5.466 0.3065 100.0 3883.1042 3883.387 5289.3 5 6.39 23.4 3 R.KAELFEIPIGILFFDLYDSEENSSKLDHILQEK.Y * Jamie_elute_b_01_itms_14.08036.08036.3 3.5578 0.3787 100.0 2776.8843 2778.0845 8016.0 3 6.077 26.1 1 K.AELFEIPIGILFFDLYDSEENSSK.L * Jamie_elute_b_01_itms_14.08029.08029.2 3.111 0.3943 100.0 2777.152 2778.0845 8828.7 1 6.111 30.4 1 K.AELFEIPIGILFFDLYDSEENSSK.L * Jamie_elute_b_01_itms_13.07181.07181.2 3.5169 0.4492 100.0 2073.8523 2075.2793 8568.4 1 7.781 52.9 2 I.PIGILFFDLYDSEENSSK.L * Jamie_elute_b_01_itms_10.05382.05382.3 5.6957 0.3913 100.0 3993.5344 3994.4292 8403.3 1 8.127 25.0 1 K.NILQSRPDISAEECNFLMVEQIDSANLTTLQNTVK.E YPL124W 5 5 15.8% 253 29280 9.5 U SPC29 SGDID:S000006045, Chr XVI from 316754-317515, Verified ORF, "Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inner plaque component Spc110p; required for SPB duplication" * Jamie_elute_b_01_itms_1.00428.00428.2 2.8224 0.3087 100.0 1759.9922 1759.0326 3189.6 1 6.154 46.7 1 R.LPPPPFSSYGMPPTNR.S * Jamie_elute_b_02_itms_5.02435.02435.2 4.1093 0.3645 100.0 1923.0322 1923.9855 6196.0 1 6.66 63.3 1 R.SSQIHIENEST*EDILK.I * Jamie_elute_b_02_itms_7.03776.03776.2 3.5141 0.3634 100.0 2036.0721 2037.1449 6369.9 14 6.418 43.8 1 R.SSQIHIENEST*EDILKI.L * Jamie_elute_b_01_itms_9.04766.04766.2 3.3057 0.2925 100.0 2325.5923 2324.4607 7209.3 2 5.513 47.4 1 R.SSQIHIENES*TEDILKILSS.S * Jamie_elute_b_02_itms_10.05480.05480.3 6.1017 0.4001 100.0 2810.9343 2809.9604 6397.8 1 6.489 43.5 1 R.SSQIHIENES*TEDILKILSSSFHN.- YIL053W 1 1 10.3% 271 30439 6.6 U RHR2 SGDID:S000001315, Chr IX from 255050-255865, Verified ORF, "Constitutively expressed isoform of DL-glycerol-3-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and, along with the Hor2p/Gpp2p isoform, osmotic stress" * Jamie_elute_b_01_itms_12.06950.06950.3 5.005 0.3882 100.0 3369.1743 3368.6748 9343.1 1 6.132 26.9 1 R.VGEYNAETDEVELIFDDYLYAKDDLLKW.- YPL131W 1 1 9.4% 297 33715 6.8 U RPL5 SGDID:S000006052, Chr XVI from 303120-304013, Verified ORF, "Protein component of the large (60S) ribosomal subunit with similarity to E. coli L18 and rat L5 ribosomal proteins; binds 5S rRNA and is required for 60S subunit assembly" * Jamie_elute_b_01_itms_11.06026.06026.3 5.7242 0.4298 100.0 3345.1743 3343.602 7562.7 1 7.45 33.3 1 R.SYIFGGHVSQYMEELADDDEERFSELFK.G Reverse_YLR375W 1 1 9.3% 343 37718 9.3 U STP3 SGDID:S000004367, Chr XII from 871696-872727, Uncharacterized ORF, "Zinc-finger protein of unknown function, possibly involved in pre-tRNA splicing and in uptake of branched-chain amino acids" * Jamie_elute_b_01_itms_9.05059.05059.3 2.9559 0.2025 90.5 3270.0842 3271.6038 7158.6 199 4.552 20.2 1 D.NSHPLLQYSLMSSSAGASGSGNSINMNPLTPH.T YOR373W 5 6 8.7% 851 94104 7.0 U NUD1 SGDID:S000005900, Chr XV from 1036830-1039385, Verified ORF, "Component of the spindle pole body outer plaque, required for exit from mitosis" * Jamie_elute_b_01_itms_3.01694.01694.3 4.0395 0.432 100.0 2020.1943 2020.1655 5797.0 1 7.912 43.1 1 K.VPSGFSGTTATSHQEAQWK.Q * Jamie_elute_b_01_itms_8.04591.04591.3 3.4791 0.245 100.0 3552.8643 3553.9507 6703.5 13 5.805 20.0 1 K.QYFPGIGSGGGTNFGGAVGTANKVPESDLIVSDLVK.D * Jamie_elute_b_01_itms_6.03058.03058.2 3.5246 0.3677 100.0 2141.9321 2143.2659 6931.7 1 6.653 50.0 1 R.VEDITSISEVNTSFNETEK.Q * Jamie_elute_b_02_itms_6.03470.03470.3 3.9188 0.3752 100.0 2222.0044 2223.2659 7168.8 1 5.881 40.3 1 R.VEDITSISEVNTS*FNETEK.Q * Jamie_elute_b_02_itms_6.03448.03448.2 3.9627 0.5149 100.0 2222.2722 2223.2659 8003.7 1 7.976 55.6 2 R.VEDITSISEVNTS*FNETEK.Q YNL064C 1 1 8.1% 409 44671 6.3 U YDJ1 SGDID:S000005008, Chr XIV from 507098-505869, reverse complement, Verified ORF, "Protein chaperone involved in regulation of the HSP90 and HSP70 functions; involved in protein translocation across membranes; member of the DnaJ family" * Jamie_elute_b_01_itms_17.09503.09503.3 5.3033 0.3626 100.0 3562.7644 3563.8965 4549.9 1 7.281 25.8 1 R.DGDDLVYEAEIDLLTAIAGGEFALEHVSGDWLK.V YIL149C 7 8 7.9% 1679 195140 6.0 U MLP2 SGDID:S000001411, Chr IX from 68067-63028, reverse complement, Verified ORF, "Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved in the Tel1p pathway that controls telomere length" * Jamie_elute_b_01_itms_4.02160.02160.2 2.2593 0.2164 92.6 1453.9321 1453.5944 4162.2 41 4.922 60.0 1 R.VKEEYDIWQSR.D * Jamie_elute_b_01_itms_12.06740.06740.3 3.4746 0.1616 92.9 3061.6743 3061.5046 7343.1 28 4.6 29.2 1 K.FQLQNQLEDFILELEHKTPELISFK.E * Jamie_elute_b_01_itms_11.06058.06058.2 4.849 0.4748 100.0 2693.652 2693.9243 6218.9 1 9.08 50.0 1 K.DANSQIQAYEEIISSNENALIELK.N * Jamie_elute_b_02_itms_8.04187.04187.3 3.4553 0.352 100.0 2544.7444 2544.862 7050.5 2 6.112 35.7 1 R.ELISNIEQTESLRVENSVLIEK.V * Jamie_elute_b_01_itms_6.03336.03336.3 4.224 0.3058 100.0 3471.2344 3472.5767 3246.8 114 5.452 25.0 2 K.GSGEDAEEELWNSPS*KGNSERPSAVAGFINQK.N * Jamie_elute_b_01_itms_4.01824.01824.3 3.7773 0.2347 100.0 1676.3944 1675.8418 6872.3 1 5.381 46.7 1 K.GNSERPSAVAGFINQK.N * Jamie_elute_b_01_itms_6.03122.03122.2 4.9792 0.5199 100.0 1983.4321 1983.099 8099.9 1 9.01 61.1 1 A.AINFSNVTDLTNNSTDGAK.I YGR159C 1 1 7.7% 414 44535 4.9 U NSR1 SGDID:S000003391, Chr VII from 807661-806417, reverse complement, Verified ORF, "Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ribosome biogenesis" * Jamie_elute_b_01_itms_12.06635.06635.3 3.5369 0.3433 100.0 3535.2844 3536.8762 5132.2 1 5.544 28.2 1 K.FGDTPSEPSDTLFLGNLSFNADRDAIFELFAK.H YPL255W 2 2 7.3% 385 45384 6.5 U BBP1 SGDID:S000006176, Chr XVI from 67725-68882, Verified ORF, "Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periphery; forms a complex with a nuclear envelope protein Mps2p and SPB components Spc29p and Kar1p; required for mitotic functions of Cdc5p" * Jamie_elute_b_01_itms_4.01827.01827.2 1.9918 0.2129 100.0 1273.2322 1273.4348 5074.7 76 5.049 60.0 1 R.SLRS*PAPIVPR.E * Jamie_elute_b_01_itms_11.06082.06082.3 4.666 0.1498 100.0 2059.2544 2059.2834 9772.1 2 4.966 53.1 1 R.DNYESEIHDLLLQLSLR.N YFR028C 2 2 6.9% 551 61907 8.0 U CDC14 SGDID:S000001924, Chr VI from 210056-208401, reverse complement, Verified ORF, "Protein phosphatase required for mitotic exit; located in the nucleolus until liberated by the FEAR and Mitotic Exit Network in anaphase, enabling it to act on key substrates to effect a decrease in CDK/B-cyclin activity and mitotic exit" * Jamie_elute_b_01_itms_13.07042.07042.2 3.6371 0.4069 100.0 2518.2122 2519.7295 9022.3 1 7.895 52.4 1 R.DAGYSNADFEITIQDVVYGVWR.A * Jamie_elute_b_02_itms_10.05277.05277.2 3.2968 0.1722 95.2 1834.0322 1835.116 8822.1 2 5.444 50.0 1 K.SVLNFFANNNVQLVVR.L YHR134W 1 1 6.7% 269 30627 8.7 U WSS1 SGDID:S000001176, Chr VIII from 371752-372561, Verified ORF, "Sumoylated protein of unknown function, identified based on genetic interactions with SMT3; UV-sensitive mutant phenotype and genetic interactions suggest a role in the DNA damage response, processing stalled or collapsed replication forks" * Jamie_elute_b_01_itms_6.03315.03315.2 2.2176 0.2789 91.7 2087.3123 2087.1619 9334.4 78 5.006 35.3 1 E.T*KNNKDQIISDNISSSLE.V YJR053W 1 1 6.4% 574 66087 5.9 U BFA1 SGDID:S000003814, Chr X from 533941-535665, Verified ORF, "Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pathways that control exit from mitosis" * Jamie_elute_b_02_itms_12.06726.06726.3 5.2277 0.4823 100.0 4170.324 4171.1685 5330.7 1 7.748 25.0 1 K.FSS*DDEGDFLTGFEELEGEAIDETISSNDKESADHPR.F YCR012W 1 1 5.5% 416 44738 7.6 U PGK1 SGDID:S000000605, Chr III from 137743-138993, Verified ORF, "3-phosphoglycerate kinase, catalyzes transfer of high-energy phosphoryl groups from the acyl phosphate of 1,3-bisphosphoglycerate to ADP to produce ATP; key enzyme in glycolysis and gluconeogenesis" * Jamie_elute_b_01_itms_12.06980.06980.2 4.3778 0.4273 100.0 2392.2522 2392.7112 6286.7 1 7.467 45.5 1 K.GVEVVLPVDFIIADAFSADANTK.T YLR044C 1 1 5.3% 563 61495 6.2 U PDC1 SGDID:S000004034, Chr XII from 234082-232391, reverse complement, Verified ORF, "Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde; subject to glucose-, ethanol-, and autoregulation; involved in amino acid catabolism" * Jamie_elute_b_01_itms_12.06527.06527.3 4.5802 0.34 100.0 3324.4744 3325.883 8911.4 1 6.229 25.0 1 K.LLQTPIDMSLKPNDAESEKEVIDTILALVK.D YJR073C 1 1 5.3% 206 23150 9.0 U OPI3 SGDID:S000003834, Chr X from 572849-572229, reverse complement, Verified ORF, "Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis" * Jamie_elute_b_02_itms_3.01523.01523.3 1.8752 0.1057 95.0 1074.2344 1074.3085 2598.5 168 4.684 42.5 1 L.GVALFGLGQVL.V YGR087C 1 2 5.2% 563 61581 6.2 U PDC6 SGDID:S000003319, Chr VII from 652985-651294, reverse complement, Verified ORF, "Minor isoform of pyruvate decarboxylase, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde, regulation is glucose- and ethanol-dependent, involved in amino acid catabolism" * Jamie_elute_b_02_itms_15.08400.08400.3 4.8012 0.0611 100.0 3084.0842 3084.4065 9856.4 3 6.728 28.6 2 K.QAVESADLILSVGALLSDFNTGSFSYSYK.T YHR170W 1 1 5.2% 518 59095 5.5 U NMD3 SGDID:S000001213, Chr VIII from 443829-445385, Verified ORF, "Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex" * Jamie_elute_b_01_itms_17.09527.09527.3 3.0166 0.2659 100.0 2890.8843 2892.2786 6632.3 7 5.436 24.0 1 R.APFNALADVTQLVEFIVLDVDSTGISR.G YDR356W 2 2 4.2% 944 111782 7.1 U SPC110 SGDID:S000002764, Chr IV from 1186098-1188932, Verified ORF, "Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner" * Jamie_elute_b_01_itms_8.04274.04274.2 2.6795 0.2776 100.0 1363.0122 1363.528 7764.1 12 5.591 65.0 1 K.NMEFT*PVGFIK.S * Jamie_elute_b_01_itms_11.05982.05982.3 5.9564 0.2668 100.0 3410.1843 3408.7441 9728.6 1 6.016 30.4 1 K.SEQSNIQHDLNLQILNLENKLIESEDELK.S YLR390W-A 1 3 4.2% 238 23268 5.9 U CCW14 SGDID:S000006429, Chr XII from 903724-904440, Verified ORF, "Covalently linked cell wall glycoprotein, present in the inner layer of the cell wall" Jamie_elute_b_02_itms_12.06834.06834.2 2.0759 0.2216 90.9 993.77216 992.9292 5525.7 112 5.028 55.6 3 K.AS*SSTKASSS.S YAL047C 2 2 4.0% 622 72105 5.1 U SPC72 SGDID:S000000045, Chr I from 56858-54990, reverse complement, Verified ORF, "Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them to microtubules; has roles in astral microtubule formation and stabilization" * Jamie_elute_b_01_itms_13.07392.07392.3 4.3755 0.2821 100.0 3034.5544 3032.4636 9373.5 1 6.199 28.1 1 K.TLDTQLEIVIEILHKEYDQFINSIR.L * Jamie_elute_b_01_itms_14.08111.08111.3 4.257 0.2785 100.0 3112.1643 3112.4636 7238.9 1 5.297 33.3 1 K.T*LDTQLEIVIEILHKEYDQFINSIR.L YAL038W 1 1 4.0% 500 54545 7.7 U CDC19 SGDID:S000000036, Chr I from 71787-73289, Verified ORF, "Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration" * Jamie_elute_b_02_itms_11.06259.06259.2 4.1928 0.4819 100.0 2266.3123 2267.581 8483.9 1 7.784 60.5 1 R.IIYVDDGVLSFQVLEVVDDK.T YGR254W 1 1 3.9% 437 46816 6.6 U ENO1 SGDID:S000003486, Chr VII from 1000932-1002245, Verified ORF, "Enolase I, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression is repressed in response to glucose" YHR174W 1 1 3.9% 437 46914 6.0 U ENO2 SGDID:S000001217, Chr VIII from 451327-452640, Verified ORF, "Enolase II, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression is induced in response to glucose" Jamie_elute_b_02_itms_11.06302.06302.2 4.0889 0.4107 100.0 1822.0322 1823.0099 9492.4 1 8.196 62.5 1 R.SGETEDTFIADLVVGLR.T YML085C 1 1 3.8% 447 49800 5.1 U TUB1 SGDID:S000004550, Chr XIII from 99400-99376,99259-97941, reverse complement, Verified ORF, "Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules" YML124C 1 1 3.8% 445 49694 5.2 U TUB3 SGDID:S000004593, Chr XIII from 23684-23660,23361-22049, reverse complement, Verified ORF, "Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p" Jamie_elute_b_01_itms_9.04860.04860.2 2.3313 0.2448 100.0 1604.7322 1607.7178 6947.0 1 5.318 46.9 1 F.T*HSLGGGTGSGLGSLLL.E Reverse_YNL164C 1 1 3.4% 351 40016 5.0 U IBD2 SGDID:S000005108, Chr XIV from 326322-325267, reverse complement, Verified ORF, "Component of the BUB2-dependent spindle checkpoint pathway, interacts with Bfa1p and functions upstream of Bub2p and Bfa1p" * Jamie_elute_b_02_itms_5.02636.02636.3 2.2904 0.2468 94.9 1441.7943 1442.5463 3890.3 37 4.705 38.6 1 R.SDQEDRPPNPMR.N YBR118W 1 1 3.3% 458 50033 9.0 U TEF2 SGDID:S000000322, Chr II from 477665-479041, Verified ORF, "Translational elongation factor EF-1 alpha; also encoded by TEF1; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA) to ribosomes" YPR080W 1 1 3.3% 458 50033 9.0 U TEF1 SGDID:S000006284, Chr XVI from 700592-701968, Verified ORF, "Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA) to ribosomes" Jamie_elute_b_02_itms_4.01766.01766.3 2.8851 0.2676 100.0 1560.3544 1561.7812 5520.9 1 4.837 44.6 1 K.SHINVVVIGHVDSGK.S Reverse_YGL133W 1 1 3.2% 1264 145642 5.7 U ITC1 SGDID:S000003101, Chr VII from 257712-261506, Verified ORF, "Component of the ATP-dependent Isw2p-Itc1p chromatin remodeling complex, required for repression of a-specific genes, repression of early meiotic genes during mitotic growth, and repression of INO1" * Jamie_elute_b_02_itms_11.06253.06253.3 1.816 0.1789 97.4 4453.2544 4456.865 7460.6 38 4.806 13.8 1 D.DKKDAGDASPTNEDTKNLKEQIAQLDGQLNSLVAAETKLER.A YKL032C 1 1 2.8% 597 67856 8.1 U IXR1 SGDID:S000001515, Chr XI from 381501-379708, reverse complement, Verified ORF, "Protein that binds DNA containing intrastrand cross-links formed by cisplatin, contains two HMG (high mobility group box) domains, which confer the ability to bend cisplatin-modified DNA; mediates aerobic transcriptional repression of COX5b" * Jamie_elute_b_01_itms_14.07736.07736.3 2.4966 0.2782 100.0 1552.8243 1548.6525 3646.5 116 4.915 31.2 1 A.AAAAANNAAGPASYLSQ.L YNR067C 1 1 2.5% 1117 121064 4.5 U DSE4 SGDID:S000005350, Chr XIV from 759099-755746, reverse complement, Verified ORF, "Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother" * Jamie_elute_b_01_itms_9.05042.05042.3 3.005 0.189 90.0 2984.2144 2982.0706 8224.1 173 4.833 22.2 1 L.DTIYVSSSMQATISSSSS*SRQTKTSSSS.L YGL008C 1 1 2.2% 918 99619 5.1 U PMA1 SGDID:S000002976, Chr VII from 482671-479915, reverse complement, Verified ORF, "Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plasma membrane potential; part of the P2 subgroup of cation-transporting ATPases" YPL036W 1 1 2.1% 947 102172 5.0 U PMA2 SGDID:S000005957, Chr XVI from 482841-485684, Verified ORF, "Plasma membrane H+-ATPase, isoform of Pma1p, involved in pumping protons out of the cell; regulator of cytoplasmic pH and plasma membrane potential" Jamie_elute_b_01_itms_13.07494.07494.2 3.3564 0.282 96.0 1987.9122 1986.3593 5574.9 1 5.087 44.7 1 R.SAADIVFLAPGLSAIIDALK.T YJR106W 1 1 2.2% 725 80657 6.8 U ECM27 SGDID:S000003867, Chr X from 624751-626928, Verified ORF, "Non-essential protein of unknown function" * Jamie_elute_b_02_itms_12.06630.06630.3 2.5764 0.2013 97.4 1494.0243 1497.7676 6427.2 137 4.765 33.3 1 C.LGGVMLNSMGGIGFSG.L YGL252C 1 1 2.2% 588 65572 8.0 U RTG2 SGDID:S000003221, Chr VII from 27484-25718, reverse complement, Verified ORF, "Sensor of mitochondrial dysfunction; regulates the subcellular location of Rtg1p and Rtg3p, transcriptional activators of the retrograde (RTG) and TOR pathways; Rtg2p is inhibited by the phosphorylated form of Mks1p" * Jamie_elute_b_02_itms_9.04820.04820.3 2.62 0.1954 95.1 1424.2144 1428.634 2833.6 11 4.649 47.9 1 P.QIKAVHFSEGGVR.E Reverse_YGL066W 1 1 2.1% 657 72878 8.9 U SGF73 SGDID:S000003034, Chr VII from 377612-379585, Verified ORF, "Probable 73 kDa subunit of SAGA histone acetyltransferase complex" * Jamie_elute_b_01_itms_11.05979.05979.2 2.1099 0.2281 92.3 1594.7122 1595.8019 5480.9 9 4.991 53.8 1 A.RKEAAAGIKTQHER.H YDR165W 1 1 1.8% 444 50488 6.7 U TRM82 SGDID:S000002572, Chr IV from 784866-786200, Verified ORF, "Subunit of a tRNA methyltransferase complex composed of Trm8p and Trm82p that catalyzes 7-methylguanosine modification of tRNA" * Jamie_elute_b_01_itms_4.01962.01962.3 1.0946 0.2834 100.0 930.1144 931.0361 1103.7 334 4.995 28.6 1 S.DGHQFIIT.S YNL271C 1 1 1.5% 1953 219701 6.5 U BNI1 SGDID:S000005215, Chr XIV from 135384-129523, reverse complement, Verified ORF, "Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1" * Jamie_elute_b_01_itms_9.04928.04928.3 2.8614 0.3521 100.0 3053.3342 3056.5166 7109.8 158 5.984 22.4 1 P.PLPSVLSSSTDGVIPPAPPMMPASQIKS*AV.T YJL020C 1 1 1.5% 1157 128296 5.3 U BBC1 SGDID:S000003557, Chr X from 402326-398853, reverse complement, Verified ORF, "Protein possibly involved in assembly of actin patches; interacts with an actin assembly factor Las17p and with the SH3 domains of Type I myosins Myo3p and Myo5p; localized predominantly to cortical actin patches" * Jamie_elute_b_01_itms_10.05274.05274.2 2.7732 0.2071 96.2 1710.9722 1711.9788 7720.9 32 5.009 40.6 1 S.RFGAPVGFNPFGMASGV.G Reverse_YKR064W 1 1 1.5% 863 101825 6.9 U YKR064W SGDID:S000001772, Chr XI from 562189-564780, Uncharacterized ORF, "Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; detectable in highly purified mitochondria" * Jamie_elute_b_02_itms_9.04676.04676.3 2.3773 0.1809 92.7 1633.4043 1631.8379 5768.1 112 4.635 41.7 1 I.RHRKKTRVQSDYG.M Reverse_YJR092W 1 1 1.3% 1448 165023 5.0 U BUD4 SGDID:S000003852, Chr X from 598649-602995, Verified ORF, "Protein involved in bud-site selection and required for axial budding pattern; localizes with septins to bud neck in mitosis and may constitute an axial landmark for next round of budding; potential Cdc28p substrate" * Jamie_elute_b_01_itms_9.05096.05096.2 1.9404 0.2421 90.0 2015.8522 2014.2087 6517.5 153 5.045 27.8 1 L.VKPSNAHGLAGNAVS*NKIR.E YGR134W 1 1 1.2% 1122 128780 5.1 U CAF130 SGDID:S000003366, Chr VII from 757775-761143, Verified ORF, "Part of the evolutionarily-conserved CCR4-NOT transcriptional regulatory complex involved in controlling mRNA initiation, elongation, and degradation" * Jamie_elute_b_01_itms_6.03168.03168.2 1.7675 0.2664 92.9 1343.5322 1342.5875 5578.8 391 4.86 41.7 1 M.VRATIRGAAALRS.V Reverse_YML065W 1 1 0.8% 914 104400 5.7 U ORC1 SGDID:S000004530, Chr XIII from 142210-144954, Verified ORF, "Largest subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; may be phosphorylated by Cdc28p" Reverse_YPR159W 1 1 1.0% 720 80123 4.7 U KRE6 SGDID:S000006363, Chr XVI from 857579-859741, Verified ORF, "Protein required for beta-1,6 glucan biosynthesis; putative beta-glucan synthase; appears functionally redundant with Skn1p" Jamie_elute_b_02_itms_7.04014.04014.2 2.0516 0.2057 95.0 855.27216 852.93726 2454.2 101 5.566 75.0 1 V.EWKSGDM.V YER172C 1 1 0.5% 2163 246183 5.5 U BRR2 SGDID:S000000974, Chr V from 536016-529525, reverse complement, Verified ORF, "RNA-dependent ATPase RNA helicase involved in the facilitation and disruption of snRNA interactions, required for disruption of U4/U6 base-pairing in native snRNPs to activate the spliceosome for catalysis" * Jamie_elute_b_02_itms_15.08521.08521.2 1.7544 0.1751 90.0 1049.5721 1049.212 4665.3 104 4.704 55.6 1 I.IGLSATLPNY.E Proteins Peptide IDs Spectra Unfiltered 7904 19923 21621 Filtered 48 89 101 Forward matches 41 82 94 Decoy matches 7 7 7 Forward FP rate 17.07 8.54 7.45 Classification Nonredundant Proteins Redundant Proteins Unclassified 0 0