Abstract
Mutation of YME genes in yeast results in a high rate of mitochondrial DNA escape to the nucleus. The synthetic respiratory growth defect of yme1 yme2 yeast strains is suppressed by recessive mutations in YNT20. Inactivation of YNT20 creates a cold-sensitive respiratory growth defect that is more pronounced in a yme1 background and which is suppressed by yme2. Inactivation of YNT20 causes a qualitative reduction in the rate of mitochondrial DNA escape in yme1, but not yme2, strains, suggesting that YNT20 plays a role in the yme1-mediated mitochondrial DNA escape pathway. YNT20p is a soluble mitochondrial protein that belongs to a subfamily of putative 3'-5' exonucleases. Furthermore, conserved sequence elements in Yme2p suggest that this protein may also function as an exonuclease.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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ATP-Dependent Proteases
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Adenosine Triphosphatases / genetics
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Amino Acid Sequence
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Base Sequence
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Chromosome Mapping
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Cloning, Molecular
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DNA Primers / genetics
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DNA, Mitochondrial / genetics
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DNA, Mitochondrial / metabolism
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Exodeoxyribonuclease V
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Exodeoxyribonucleases / genetics*
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Exodeoxyribonucleases / metabolism
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Genes, Fungal
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Mitochondria / enzymology
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Molecular Sequence Data
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Mutation
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Phenotype
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Saccharomyces cerevisiae / enzymology*
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae / metabolism
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Saccharomyces cerevisiae Proteins*
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Sequence Homology, Amino Acid
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Suppression, Genetic
Substances
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DNA Primers
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DNA, Mitochondrial
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Saccharomyces cerevisiae Proteins
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Exodeoxyribonucleases
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Exodeoxyribonuclease V
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ATP-Dependent Proteases
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YME1 protein, S cerevisiae
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Adenosine Triphosphatases