YNT20, a bypass suppressor of yme1 yme2, encodes a putative 3'-5' exonuclease localized in mitochondria of Saccharomyces cerevisiae

Curr Genet. 1999 Jan;34(6):438-48. doi: 10.1007/s002940050418.

Abstract

Mutation of YME genes in yeast results in a high rate of mitochondrial DNA escape to the nucleus. The synthetic respiratory growth defect of yme1 yme2 yeast strains is suppressed by recessive mutations in YNT20. Inactivation of YNT20 creates a cold-sensitive respiratory growth defect that is more pronounced in a yme1 background and which is suppressed by yme2. Inactivation of YNT20 causes a qualitative reduction in the rate of mitochondrial DNA escape in yme1, but not yme2, strains, suggesting that YNT20 plays a role in the yme1-mediated mitochondrial DNA escape pathway. YNT20p is a soluble mitochondrial protein that belongs to a subfamily of putative 3'-5' exonucleases. Furthermore, conserved sequence elements in Yme2p suggest that this protein may also function as an exonuclease.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • ATP-Dependent Proteases
  • Adenosine Triphosphatases / genetics
  • Amino Acid Sequence
  • Base Sequence
  • Chromosome Mapping
  • Cloning, Molecular
  • DNA Primers / genetics
  • DNA, Mitochondrial / genetics
  • DNA, Mitochondrial / metabolism
  • Exodeoxyribonuclease V
  • Exodeoxyribonucleases / genetics*
  • Exodeoxyribonucleases / metabolism
  • Genes, Fungal
  • Mitochondria / enzymology
  • Molecular Sequence Data
  • Mutation
  • Phenotype
  • Saccharomyces cerevisiae / enzymology*
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / metabolism
  • Saccharomyces cerevisiae Proteins*
  • Sequence Homology, Amino Acid
  • Suppression, Genetic

Substances

  • DNA Primers
  • DNA, Mitochondrial
  • Saccharomyces cerevisiae Proteins
  • Exodeoxyribonucleases
  • Exodeoxyribonuclease V
  • ATP-Dependent Proteases
  • YME1 protein, S cerevisiae
  • Adenosine Triphosphatases