The Saccharomyces cerevisiae succinate-ubiquinone reductase contains a stoichiometric amount of cytochrome b562

FEBS Lett. 1999 Jan 15;442(2-3):203-7. doi: 10.1016/s0014-5793(98)01657-3.

Abstract

The Saccharomyces cerevisiae succinate-ubiquinone reductase or succinate dehydrogenase (SDH) is a tetramer of non-equivalent subunits encoded by the SDH1, SDH2, SDH3, and SDH4 genes. In most organisms, SDH contains one or two endogenous b-type hemes. However, it is widely believed that the yeast SDH does not contain heme. In this report, we demonstrate the presence of a stoichiometric amount of cytochrome b562 in the yeast SDH. The cytochrome is detected as a peak present in fumarate-oxidized, dithionite-reduced mitochondria. The peak is centered at 562 nm and is present at a heme:covalent FAD molar ratio of 0.92+/-0.11. The cytochrome is not detectable in mitochondria isolated from SDH3 and SDH4 deletion strains. These observations strongly support our conclusion that cytochrome b562 is a component of the yeast SDH.

MeSH terms

  • Animals
  • Cytochrome b Group / analysis*
  • Dithionite / metabolism
  • Electron Transport Complex II
  • Escherichia coli Proteins*
  • Flavin-Adenine Dinucleotide / metabolism
  • Fumarates / metabolism
  • Fungal Proteins / genetics
  • Gene Deletion
  • Intracellular Membranes / drug effects
  • Intracellular Membranes / enzymology
  • Intracellular Membranes / metabolism
  • Lactic Acid / metabolism
  • Malonates / pharmacology
  • Mice
  • Mitochondria / drug effects
  • Mitochondria / enzymology*
  • Mitochondria / genetics
  • Mitochondria / metabolism
  • Multienzyme Complexes / chemistry*
  • Oxidation-Reduction / drug effects
  • Oxidoreductases / chemistry*
  • Saccharomyces cerevisiae / drug effects
  • Saccharomyces cerevisiae / enzymology*
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism
  • Spectrum Analysis
  • Succinate Dehydrogenase / chemistry*

Substances

  • Cytochrome b Group
  • Escherichia coli Proteins
  • Fumarates
  • Fungal Proteins
  • Malonates
  • Multienzyme Complexes
  • Flavin-Adenine Dinucleotide
  • Dithionite
  • Lactic Acid
  • fumaric acid
  • cytochrome b562, E coli
  • malonic acid
  • Oxidoreductases
  • Electron Transport Complex II
  • Succinate Dehydrogenase