Abstract
The SAGA histone acetyltransferase/transcriptional adaptor complex is composed of multiple transcriptional regulators including Ada, Spt, and TAFII proteins. Here we identify an additional novel subunit of the complex, Tra1, an ATM/PI-3-kinase-related homolog of the human TRRAP cofactor, which is essential for c-Myc and E2F-mediated oncogenic transformation. Mass spectrometry, immunoblotting, and immunoprecipitation experiments confirm the stable association of this protein within SAGA. In addition, the Tra1 protein is a component of at least two other histone acetyltransferase protein complexes. These results indicate a role for Tra1 in the regulation of transcriptional activation through the recruitment of HAT activity to an activator-bound promoter.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Acetyltransferases / metabolism*
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Amino Acid Sequence
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Ataxia Telangiectasia Mutated Proteins
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Cell Cycle Proteins
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DNA-Binding Proteins*
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Electrophoresis, Polyacrylamide Gel
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Fungal Proteins / physiology
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Histone Acetyltransferases
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Immunoblotting
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Molecular Sequence Data
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Multienzyme Complexes / chemistry
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Multienzyme Complexes / isolation & purification
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Multienzyme Complexes / metabolism
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Protein Kinases / physiology
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Protein Serine-Threonine Kinases*
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Proteins / analysis*
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Proteins / metabolism*
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Saccharomyces cerevisiae / chemistry
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Saccharomyces cerevisiae / enzymology
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Saccharomyces cerevisiae Proteins*
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Tumor Suppressor Proteins
Substances
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Cell Cycle Proteins
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DNA-Binding Proteins
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Fungal Proteins
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Multienzyme Complexes
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Proteins
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Saccharomyces cerevisiae Proteins
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TRA1 protein, S cerevisiae
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Tumor Suppressor Proteins
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Acetyltransferases
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Histone Acetyltransferases
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Protein Kinases
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ATM protein, human
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Ataxia Telangiectasia Mutated Proteins
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Protein Serine-Threonine Kinases