Abstract
Deletion of the telomerase catalytic subunit gene trt1+ in Schizosaccharomyces pombe results in death for the majority of cells, but a subpopulation survives. Here it is shown that most survivors have circularized all of their chromosomes, whereas a smaller number maintain their telomeres presumably through recombination. When the telomeric DNA-binding gene taz1+ is also deleted, trt1- taz1- survivors use the recombinational mode more frequently. Moreover, the massive elongation of telomeres in taz1- cells is absent in the double mutant. Thus, Taz1p appears to regulate telomeric recombination as well as telomerase activity in fission yeast.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Chromosomes, Fungal / genetics
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Chromosomes, Fungal / metabolism*
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DNA Probes
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DNA, Fungal / metabolism
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism
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Gene Deletion
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Genes, Fungal
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Proteins / genetics
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Proteins / metabolism*
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RNA*
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Recombination, Genetic*
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Schizosaccharomyces / enzymology
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Schizosaccharomyces / genetics
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Schizosaccharomyces / physiology*
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Schizosaccharomyces pombe Proteins*
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Telomerase / genetics
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Telomerase / metabolism
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Telomere / genetics*
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Telomere / metabolism*
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Telomere-Binding Proteins*
Substances
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DNA Probes
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DNA, Fungal
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DNA-Binding Proteins
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Proteins
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Schizosaccharomyces pombe Proteins
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Telomere-Binding Proteins
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taz1 protein, S pombe
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telomerase RNA
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trt1 protein, S pombe
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RNA
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Telomerase