Disruption studies of a Candida albicans gene, ELF1: a member of the ATP-binding cassette family

Microbiology (Reading). 1998 Aug:144 ( Pt 8):2311-2321. doi: 10.1099/00221287-144-8-2311.

Abstract

A 3.6 kb gene (ELF1) with homology to the ATP-binding cassette (ABC) gene family has been isolated from genomic libraries of Candida albicans. Members of this gene family include both membrane transport proteins which confer a drug-resistance phenotype, and proteins whose functions are associated with protein translation. ELF1 (Elongation Like Factor) showed greatest homology with a Saccharomyces cerevisiae ORF (YPL226W), whose function is unknown, and lower homology with fungal elongation factor 3 (EF-3) genes. In comparison, homology with a gene conferring a drug-resistant phenotype (CDR1) was low. To understand the function of ELF1 in C. albicans, gene-knockout experiments were conducted using the hisG-URA3-hisG disruption cassette. Both single-copy (heterozygote) and double-disrupted strains in ELF1 were isolated. Phenotypically, the disrupted strains grew more slowly than wild-type and produced a mixture of large, irregular cells and apparently normal cells.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • ATP-Binding Cassette Transporters / genetics*
  • ATP-Binding Cassette Transporters / isolation & purification
  • Adenosine Triphosphate / metabolism*
  • Amino Acid Sequence
  • Candida albicans / genetics*
  • Fungal Proteins / genetics*
  • Fungal Proteins / isolation & purification
  • Genes, Fungal
  • Molecular Sequence Data
  • Mutagenesis, Site-Directed / genetics
  • Peptide Elongation Factors / genetics
  • Phenotype
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae Proteins
  • Schizosaccharomyces pombe Proteins*
  • Transformation, Genetic*

Substances

  • ATP-Binding Cassette Transporters
  • Elf1 protein, S pombe
  • Fungal Proteins
  • Peptide Elongation Factors
  • Saccharomyces cerevisiae Proteins
  • Schizosaccharomyces pombe Proteins
  • YEF3 protein, S cerevisiae
  • Adenosine Triphosphate

Associated data

  • GENBANK/AF038153