Abstract
Seven transmembrane domain receptors can be localized to different parts of the plasma membrane or to different intracellular compartments in a receptor-specific and cell type-specific fashion. We show here that the C. elegans genes odr-4 and odr-8 are required for localization of a subset of seven transmembrane domain odorant receptors to the cilia of olfactory neurons. Other cilia-signaling proteins, including ion channels, a G alpha protein, and even other receptor types, are localized via an odr-4/odr-8-independent pathway. odr-4 encodes a novel membrane protein that is expressed exclusively on intracellular membranes of chemosensory neurons, where it acts cell-autonomously to facilitate odorant receptor folding or localization.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, Non-P.H.S.
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Amino Acid Sequence
-
Animals
-
Caenorhabditis elegans / chemistry*
-
Caenorhabditis elegans / genetics
-
Caenorhabditis elegans Proteins*
-
Chemotaxis / genetics
-
Chromosome Mapping
-
Cilia / chemistry
-
Cloning, Molecular
-
Genes, Helminth / genetics
-
Membrane Proteins / analysis
-
Molecular Sequence Data
-
Mutation / genetics
-
Odorants
-
Olfactory Receptor Neurons / chemistry*
-
Promoter Regions, Genetic / genetics
-
Receptors, Odorant / analysis*
-
Receptors, Odorant / genetics
-
Receptors, Odorant / physiology*
-
Recombinant Fusion Proteins
Substances
-
Caenorhabditis elegans Proteins
-
Membrane Proteins
-
ODR-10 protein, C elegans
-
ODR-4 protein, C elegans
-
Receptors, Odorant
-
Recombinant Fusion Proteins