Abstract
The Apn1 protein of the budding yeast Saccharomyces cerevisiae is a DNA repair enzyme that hydrolyzes apurinic/apyrimidinic (AP) sites and removes 3'-blocking groups present at single strand breaks of damaged DNA. Yeast cells lacking Apn1 are hypersensitive to DNA damaging agents that produce AP sites and DNA strand breaks with blocked 3'-termini. In this study, we showed that the fission yeast Schizosaccharomyces pombe bears a homologue, Spapn1, that is 45% identical to S. cerevisiae Apn1. However, the Spapn1 gene is apparently not expressed. Active expression of S. cerevisiae Apn1 in S. pombe conferred no additional resistance to DNA damaging agents. These data suggest that the pathway by which S. pombe repairs AP sites is independent of a functional Apn1-like AP endonuclease.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Caenorhabditis elegans / genetics
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Carbon-Oxygen Lyases / genetics*
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Cloning, Molecular
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DNA Repair Enzymes
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DNA Repair*
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DNA-(Apurinic or Apyrimidinic Site) Lyase
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Deoxyribonuclease IV (Phage T4-Induced)
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Endodeoxyribonucleases / genetics*
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Escherichia coli / genetics
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Escherichia coli Proteins*
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Molecular Sequence Data
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Multigene Family*
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Saccharomyces cerevisiae / enzymology
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae Proteins*
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Schizosaccharomyces / enzymology
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Schizosaccharomyces / genetics*
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Sequence Homology, Amino Acid*
Substances
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Escherichia coli Proteins
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Saccharomyces cerevisiae Proteins
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Endodeoxyribonucleases
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Deoxyribonuclease IV (Phage T4-Induced)
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endonuclease IV, E coli
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Apn1 protein, S cerevisiae
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Carbon-Oxygen Lyases
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DNA-(Apurinic or Apyrimidinic Site) Lyase
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DNA Repair Enzymes