Pex14p is a member of the protein linkage map of Pex5p

C Brocard; G Lametschwandtner; R Koudelka; A Hartig

doi:10.1093/emboj/16.18.5491

Pex14p is a member of the protein linkage map of Pex5p

EMBO J. 1997 Sep 15;16(18):5491-500. doi: 10.1093/emboj/16.18.5491.

Authors

C Brocard¹, G Lametschwandtner, R Koudelka, A Hartig

Affiliation

¹ Institut für Biochemie und Molekulare Zellbiologie der Universität Wien and Ludwig Boltzmann-Forschungsstelle für Biochemie, Vienna Biocenter, Dr. Bohrgasse 9, A-1030 Wien, Austria.

Abstract

To identify members of the translocation machinery for peroxisomal proteins, we made use of the two-hybrid system to establish a protein linkage map centered around Pex5p from Saccharomyces cerevisiae, the receptor for the C-terminal peroxisomal targeting signal (PTS1). Among the five interaction partners identified, Pex14p was found to be induced under conditions allowing peroxisome proliferation. Deletion of the corresponding gene resulted in the inability of yeast cells to grow on oleate as well as the absence of peroxisomal structures. The PEX14 gene product of approximately 38 kDa was biochemically and ultrastructurally demonstrated to be a peroxisomal membrane protein, despite the lack of a membrane-spanning domain. This protein was shown to interact with itself, with Pex13p and with both PTS receptors, Pex5p and Pex7p, indicating a central function for the import of peroxisomal matrix proteins, either as a docking protein or as a releasing factor at the organellar membrane.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Base Sequence
Carrier Proteins*
Cloning, Molecular
Escherichia coli
Fungal Proteins / chemistry
Fungal Proteins / metabolism*
Fungal Proteins / ultrastructure
Gene Deletion
Membrane Proteins / chemistry
Membrane Proteins / metabolism*
Membrane Proteins / ultrastructure
Membrane Transport Proteins
Microbodies / metabolism*
Microbodies / ultrastructure
Molecular Sequence Data
Peroxins
Peroxisome-Targeting Signal 1 Receptor
Receptors, Cytoplasmic and Nuclear / metabolism*
Recombinant Proteins / chemistry
Recombinant Proteins / metabolism
Recombinant Proteins / ultrastructure
Repressor Proteins*
Saccharomyces cerevisiae / genetics
Saccharomyces cerevisiae / metabolism*
Saccharomyces cerevisiae / ultrastructure
Saccharomyces cerevisiae Proteins

Substances

Carrier Proteins
Fungal Proteins
Membrane Proteins
Membrane Transport Proteins
PEX14 protein, S cerevisiae
Peroxins
Peroxisome-Targeting Signal 1 Receptor
Receptors, Cytoplasmic and Nuclear
Recombinant Proteins
Repressor Proteins
Saccharomyces cerevisiae Proteins