Abstract
The SGS1 gene of yeast encodes a DNA helicase with homology to the human WRN gene. Mutations in WRN result in Werner's syndrome, a disease with symptoms resembling premature aging. Mutation of SGS1 is shown to cause premature aging in yeast mother cells on the basis of a shortened life-span and the aging-induced phenotypes of sterility and redistribution of the Sir3 silencing protein from telomeres to the nucleolus. Further, in old sgs1 cells the nucleolus is enlarged and fragmented-changes that also occur in old wild-type cells. These findings suggest a conserved mechanism of cellular aging that may be related to nucleolar structure.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Cell Division
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Cell Nucleolus / chemistry
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Cell Nucleolus / metabolism
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Cell Nucleolus / ultrastructure*
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Cellular Senescence*
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DNA Helicases / genetics*
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DNA Helicases / physiology
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Exodeoxyribonucleases
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Fluorescent Antibody Technique, Indirect
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Fungal Proteins / analysis
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Genes, Fungal
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Humans
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Mutation
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Phenotype
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RecQ Helicases
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Saccharomyces cerevisiae / cytology*
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae / physiology
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Saccharomyces cerevisiae / ultrastructure
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Saccharomyces cerevisiae Proteins
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Silent Information Regulator Proteins, Saccharomyces cerevisiae*
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Trans-Activators / analysis
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Werner Syndrome / genetics
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Werner Syndrome Helicase
Substances
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Fungal Proteins
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SIR3 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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Silent Information Regulator Proteins, Saccharomyces cerevisiae
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Trans-Activators
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Exodeoxyribonucleases
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SGS1 protein, S cerevisiae
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DNA Helicases
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RecQ Helicases
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WRN protein, human
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Werner Syndrome Helicase